EP1589802A1 - Method for treating seeds, and seeds obtainable with the method - Google Patents

Method for treating seeds, and seeds obtainable with the method

Info

Publication number
EP1589802A1
EP1589802A1 EP04706390A EP04706390A EP1589802A1 EP 1589802 A1 EP1589802 A1 EP 1589802A1 EP 04706390 A EP04706390 A EP 04706390A EP 04706390 A EP04706390 A EP 04706390A EP 1589802 A1 EP1589802 A1 EP 1589802A1
Authority
EP
European Patent Office
Prior art keywords
seeds
temperature
seconds
carried out
medium
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP04706390A
Other languages
German (de)
French (fr)
Other versions
EP1589802B1 (en
Inventor
Hendricus Cornelis Van Deventer
Albert Van Duijn
Rudy Mathias Henricus Heijmans
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nederlandse Organisatie voor Toegepast Natuurwetenschappelijk Onderzoek TNO
Original Assignee
Nederlandse Organisatie voor Toegepast Natuurwetenschappelijk Onderzoek TNO
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nederlandse Organisatie voor Toegepast Natuurwetenschappelijk Onderzoek TNO filed Critical Nederlandse Organisatie voor Toegepast Natuurwetenschappelijk Onderzoek TNO
Publication of EP1589802A1 publication Critical patent/EP1589802A1/en
Application granted granted Critical
Publication of EP1589802B1 publication Critical patent/EP1589802B1/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23B—PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
    • A23B9/00—Preservation of edible seeds, e.g. cereals
    • A23B9/005—Processes or apparatus using pressure variation or mechanical force, e.g. shock, acceleration, shear stress, contortion
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01C—PLANTING; SOWING; FERTILISING
    • A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
    • A01C1/08—Immunising seed
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23B—PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
    • A23B9/00—Preservation of edible seeds, e.g. cereals
    • A23B9/02—Preserving by heating
    • A23B9/025—Preserving by heating with use of gases

Definitions

  • the invention relates to a method for treating seeds, and seeds obtainable with the method.
  • seeds are subjected to a sterilization treatment in order to kill the pathogens present on the seeds so that the transmission of pathogens to the crop to grow from the seeds is avoided and harm to the seeds themselves is prevented.
  • a sterilization treatment also increases the storage life of seeds. This applies to both seeds for sowing and seeds which are, possibly after germination, suitable for consumption or industrial processing. For seeds intended for reproduction or for consumption or industrial processing after the start of the germination process, it is logically of vital importance that the germination capacity, or the enzyme activity, is preserved.
  • Seeds suitable for direct consumption are usually treated under such conditions that all germination capacity, if present at all, is completely lost.
  • Seeds whose germination capacity needs to be preserved are nowadays treated with chemicals by, means of which the number of pathogens on the surface of the seeds is reduced, while the germination capacity can indeed be preserved.
  • chemicals particularly for biological cultivation, and for seeds which are consumed or processed when germinated, the use of such chemicals is highly undesired, while the norms regarding chemical additions for traditional seed production are becoming increasingly stringent.
  • the object of the present invention is therefore to provide a method for treating seeds, in which the seeds are sterilized without chemicals needing to be used, and in which at least a considerable extent of the germination capacity of the seeds is preserved or even increases.
  • the invention thus relates to a method for treating seeds, comprising the following steps:
  • step (b) a cooling step in which the sterilized seeds obtained in step (a) are cooled, wherein the temperature of the surface of the seeds is lowered, within 30 seconds at most, to 50°C at most, and the conditions of steps (a) and (b) are set such that the germination capacity of the seeds is relatively preserved or increases.
  • the seeds obtained with the method according to the invention exhibit a long storage life, while the germination capacity is at least to a considerable extent preserved or even increases. Very surprisingly, it has been observed that the seeds treated with the method according to the invention often have a better germination capacity than untreated seeds.
  • a "relative preservation of germination capacity” is understood to mean that the germination capacity as such is usually preserved, but that, in certain cases, the germination capacity can decrease by 25% at most, that is, a minimum preservation of 75% of the germination capacity such as it is present before the treatment.
  • the condensing medium condenses on the surface of cold seed in step (a), the surface being very rapidly and uniformly heated, after which, before the heat can penetrate the seed, in step (b), the formed condensate rapidly evaporates so that the surface of the seed is cooled.
  • the condensing medium may, for instance, consist of steam, but other media having a condensation temperature of 80-200°C are also suitable.
  • step (a) the parameters to be selected for step (a), such as time, pressure and temperature, depend on the size of the seeds, the type of seed and the properties of the condensing medium. For instance, a certain type of seed having larger dimensions may usually be exposed to a steam treatment longer, while preserving germination capacity, than a type of seed having smaller dimensions.
  • Step (a) is carried out for 0.1-60 seconds, preferably for 0.1-5 seconds, and still more preferably for 0.5-1.5 seconds.
  • the surface of the seeds will reach a temperature of 200°C at most, before the seed parts are subjected to step (b).
  • the surface of the seeds reaches a temperature of 80-150°C.
  • Step (a) is preferably carried out at a pressure of 0.5-20 bar absolute, still more preferably a pressure of 1-5 bar absolute.
  • the seeds are cooled by means of a vacuum treatment or another manner in which rapid evaporation occurs.
  • Step (b) is carried out such that, within 30 seconds, the temperature of the surface of the seeds is lowered to 50°C at most.
  • step (b) is carried out such that, within 10 seconds, the temperature of the surface of the seeds is lowered to 30°C at most.
  • step (b) is carried out for 0.5-30 seconds, more preferably for 0.5-5 seconds, and still more preferably for 0.5-1 seconds.
  • step (a) preferably, steam is used as the condensing medium.
  • the condensing medium may optionally be superheated.
  • the initial temperature of the seeds is preferably -30-30°C, more preferably 0-30°C and still more preferably 10-25°C.
  • the initial temperature of the seeds is understood to mean the temperature of the seeds just before or at the moment when they are subjected to the sterilization step.
  • the method according to the invention is carried out such that the temperature of the germinating part of the seeds does not greatly exceed the initial temperature of the seeds.
  • the initial temperature of the germinating part of the seeds is essentially preserved.
  • a small increase of the initial temperature will not directly have a great adverse influence on the germination capacity of the seeds, and sometimes even a positive influence, it will be clear that the germination capacity of the seeds will increasingly be harmed according as the temperature of the germinating part of the seeds more greatly exceeds the initial temperature during the treatment.
  • the harm to the germination capacity depends on the maximum temperatures reached by the germinating parts of the seeds and the time during which these temperatures occur. This will differ per type of seed.
  • seeds can be treated which are suitable for consumption or industrial processing.
  • the seeds to be treated with the method according to the invention are those plant parts which can be used for reproduction and can be chosen from the group of seed kernels, bulbs, tubers, cuttings and rhizomes, but are not limited to this group.
  • the invention further relates to seeds which can be obtained by means of the method according to the invention.
  • Figs. 1 and 2 show the results of the number of colonies that could be counted on the Petri dishes and the maximum number of seeds that germinated after each treatment. Fig.
  • Fig. 1 clearly shows that the number of microorganisms on the seeds decreased when the seeds were treated with the method according to the invention
  • Fig. 2 clearly shows that the treatment according to the invention had a positive influence on the germination capacity of the seeds when the treatment took place for a relatively short time.

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Chemical & Material Sciences (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Mechanical Engineering (AREA)
  • Soil Sciences (AREA)
  • Environmental Sciences (AREA)
  • Pretreatment Of Seeds And Plants (AREA)

Abstract

The invention relates to a method for treating seeds, comprising the following steps: (a) a sterilization step wherein, for 0.1 to 60 seconds and at a minimum pressure of 0.5 bar absolute, the seeds are contacted with a condensing medium having a condensation temperature of 80-200°C, wherein the medium condenses on the seeds and the temperature of the surface of the seeds is raised to 200°C at most; and (b) a cooling step wherein the sterilized seeds obtained in step (a) are cooled, wherein the temperature of the surface of the seeds is lowered, within 30 seconds at most, to 50°C at most, and wherein the conditions of steps (a) and (b) are set such that the germination capacity of the seeds is relatively preserved or increases. The invention further relates to seeds obtainable with the method according to the invention.

Description

Title: Method for treating seeds, and seeds obtainable with the method
The invention relates to a method for treating seeds, and seeds obtainable with the method.
In general, seeds are subjected to a sterilization treatment in order to kill the pathogens present on the seeds so that the transmission of pathogens to the crop to grow from the seeds is avoided and harm to the seeds themselves is prevented. Such a treatment also increases the storage life of seeds. This applies to both seeds for sowing and seeds which are, possibly after germination, suitable for consumption or industrial processing. For seeds intended for reproduction or for consumption or industrial processing after the start of the germination process, it is logically of vital importance that the germination capacity, or the enzyme activity, is preserved.
Seeds suitable for direct consumption are usually treated under such conditions that all germination capacity, if present at all, is completely lost. In this context, reference can be made to, for instance, international patent application WO 96/20606, in which seeds, herbs and spices are sterilized by means of a heat treatment which may, for instance, be carried out with steam. While the taste and smell of the seeds which are subjected to such a treatment are well preserved, the germination capacity of seeds which are subjected to such a treatment is completely lost.
Seeds whose germination capacity needs to be preserved, by contrast, are nowadays treated with chemicals by, means of which the number of pathogens on the surface of the seeds is reduced, while the germination capacity can indeed be preserved. However, particularly for biological cultivation, and for seeds which are consumed or processed when germinated, the use of such chemicals is highly undesired, while the norms regarding chemical additions for traditional seed production are becoming increasingly stringent.
The object of the present invention is therefore to provide a method for treating seeds, in which the seeds are sterilized without chemicals needing to be used, and in which at least a considerable extent of the germination capacity of the seeds is preserved or even increases.
It has now surprisingly been found that this can be achieved by a combination of a specific sterilization step and a specific cooling step.
The invention thus relates to a method for treating seeds, comprising the following steps:
(a) a sterilization step in which, for 0.1 to 60 seconds and at a minimum pressure of 0.5 bar absolute, the seeds are contacted with a medium having a condensation temperature of 80-200°C, wherein the medium condenses on the seeds and the temperature of the surface of the seeds is raised to 200°C at most; and
(b) a cooling step in which the sterilized seeds obtained in step (a) are cooled, wherein the temperature of the surface of the seeds is lowered, within 30 seconds at most, to 50°C at most, and the conditions of steps (a) and (b) are set such that the germination capacity of the seeds is relatively preserved or increases.
The seeds obtained with the method according to the invention exhibit a long storage life, while the germination capacity is at least to a considerable extent preserved or even increases. Very surprisingly, it has been observed that the seeds treated with the method according to the invention often have a better germination capacity than untreated seeds.
In the context of the present invention a "relative preservation of germination capacity" is understood to mean that the germination capacity as such is usually preserved, but that, in certain cases, the germination capacity can decrease by 25% at most, that is, a minimum preservation of 75% of the germination capacity such as it is present before the treatment. In the use of the present invention, the condensing medium condenses on the surface of cold seed in step (a), the surface being very rapidly and uniformly heated, after which, before the heat can penetrate the seed, in step (b), the formed condensate rapidly evaporates so that the surface of the seed is cooled.
The condensing medium may, for instance, consist of steam, but other media having a condensation temperature of 80-200°C are also suitable.
A skilled person will understand that the parameters to be selected for step (a), such as time, pressure and temperature, depend on the size of the seeds, the type of seed and the properties of the condensing medium. For instance, a certain type of seed having larger dimensions may usually be exposed to a steam treatment longer, while preserving germination capacity, than a type of seed having smaller dimensions.
Step (a) is carried out for 0.1-60 seconds, preferably for 0.1-5 seconds, and still more preferably for 0.5-1.5 seconds. In step (a), the surface of the seeds will reach a temperature of 200°C at most, before the seed parts are subjected to step (b). Preferably, in step (a), the surface of the seeds reaches a temperature of 80-150°C.
Step (a) is preferably carried out at a pressure of 0.5-20 bar absolute, still more preferably a pressure of 1-5 bar absolute.
Preferably, in step (b), the seeds are cooled by means of a vacuum treatment or another manner in which rapid evaporation occurs.
Step (b) is carried out such that, within 30 seconds, the temperature of the surface of the seeds is lowered to 50°C at most. Preferably, step (b) is carried out such that, within 10 seconds, the temperature of the surface of the seeds is lowered to 30°C at most.
Preferably, step (b) is carried out for 0.5-30 seconds, more preferably for 0.5-5 seconds, and still more preferably for 0.5-1 seconds. In step (a), preferably, steam is used as the condensing medium. The condensing medium may optionally be superheated. The initial temperature of the seeds is preferably -30-30°C, more preferably 0-30°C and still more preferably 10-25°C.
For the sake of completeness, it is noted that all above-mentioned limit ranges comprise both the limit values mentioned above and below. It will be clear to a skilled person that the initial temperature of the seeds is understood to mean the temperature of the seeds just before or at the moment when they are subjected to the sterilization step. Preferably, the method according to the invention is carried out such that the temperature of the germinating part of the seeds does not greatly exceed the initial temperature of the seeds.
Preferably, the initial temperature of the germinating part of the seeds is essentially preserved. Although a small increase of the initial temperature will not directly have a great adverse influence on the germination capacity of the seeds, and sometimes even a positive influence, it will be clear that the germination capacity of the seeds will increasingly be harmed according as the temperature of the germinating part of the seeds more greatly exceeds the initial temperature during the treatment. The harm to the germination capacity depends on the maximum temperatures reached by the germinating parts of the seeds and the time during which these temperatures occur. This will differ per type of seed. By means of the method according to the present invention, also, seeds can be treated which are suitable for consumption or industrial processing.
The seeds to be treated with the method according to the invention are those plant parts which can be used for reproduction and can be chosen from the group of seed kernels, bulbs, tubers, cuttings and rhizomes, but are not limited to this group. The invention further relates to seeds which can be obtained by means of the method according to the invention. Example 1
In experiments 1-10, barley seeds were treated with the method according to the invention. The duration and pressure in steps (a) and (b) were varied, and these are shown in Table 1. In experiments 1-5, the initial temperature of the cores of the seeds was 20°C, while in experiments 6-10 the initial temperature of the cores of the seeds was -20°C. In all experiments, the minimum supply temperature of the steam was 120°C, and from experiment 5, the steam was superheated to 160°C. It was then determined to what extent the treatment according to the invention had influenced the number of microorganisms on the seed, and what the effect on the germination of the seed was. As a control, also, the contents of microorganisms and the germination were determined of barley seeds which were not subjected to the treatment according to the invention, and whose initial temperature was 20°C and -20°C, respectively.
Table 1
After each treatment, the seeds (3g, approximately 60 seeds) were shaken for 3 hours with 10 ml of sterile demineralized water. Subsequently, 100 μl of a 1:1000 dilution of the water was spread on an LB plate and, for two days, incubation of the LB plates took place in an incubator at a temperature of 37°C. After this, the seeds obtained with the treatments were placed to germinate in Petri dishes between 2x2 layers of filter paper (with 3 and 2.5 ml of water, respectively). Incubation in the dark took place at 20°C for one week. Figs. 1 and 2 show the results of the number of colonies that could be counted on the Petri dishes and the maximum number of seeds that germinated after each treatment. Fig. 1 clearly shows that the number of microorganisms on the seeds decreased when the seeds were treated with the method according to the invention, while Fig. 2 clearly shows that the treatment according to the invention had a positive influence on the germination capacity of the seeds when the treatment took place for a relatively short time.

Claims

1. A method for treating seeds, comprising the following steps:
(a) a sterilization step wherein, for 0.1 to 60 seconds and at a minimum pressure of 0.5 bar absolute, the seeds are contacted with a medium having a condensation temperature of 80-200°C, wherein the medium condenses on the seeds and the temperature of the surface of the seeds is raised to 200°C at most; and
(b) a cooling step wherein the sterilized seeds obtained in step (a) are cooled, wherein the temperature of the surface is lowered, within 30 seconds at most, to 50°C at most, and wherein the conditions of steps (a) and (b) are set such that the germination capacity of the seeds is relatively preserved or increases.
2. A method according to claim 1, wherein step (a) is carried out for 0.1-5 seconds.
3. A method according to claim 2, wherein step (a) is carried out for 0.5-1.5 seconds.
4. A method according to any one of claims 1-3, wherein, in step (a), the surface of the seeds reaches a temperature of 80-150°C, before the seeds are subjected to step (b).
5. A method according to any one of claims 1-4, wherein step (a) is carried out at a pressure of 0.5-20 bar absolute.
6. A method according to any one of claims 1-5, wherein, in step (b), the seeds are cooled by means of a vacuum treatment or another manner in which rapid evaporation occurs.
7. A method according to any one of claims 1-6, wherein step (b) is carried out such that, within 10 seconds, the temperature of the surface of the seeds is lowered to 30°C at most.
8. A method according to claim 7, wherein step (b) is carried out for 0.5-5 seconds.
9. A method according to any one of claims 1-8, wherein, in step (a), steam is used as the condensing medium.
10. A method according to any one of claims 1-9, wherein the seeds have an initial temperature of 0-30°C.
11. A method according to any one of claims 1-10, wherein the seeds have been chosen from the group of seed kernels, bulbs, tubers, cuttings, and rhizomes.
12. A method according to any one of claims 1-11, wherein the seeds involved are seeds suitable for consumption or industrial processing.
13. Seeds obtainable with the method according to any one of claims 1-12.
EP04706390.4A 2003-01-31 2004-01-29 Method for treating seeds, and seeds obtainable with the method Expired - Lifetime EP1589802B1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
NL1022549A NL1022549C2 (en) 2003-01-31 2003-01-31 Method for treating seeds, and seeds that can be obtained with the method.
NL1022549 2003-01-31
PCT/NL2004/000066 WO2004066710A1 (en) 2003-01-31 2004-01-29 Method for treating seeds, and seeds obtainable with the method

Publications (2)

Publication Number Publication Date
EP1589802A1 true EP1589802A1 (en) 2005-11-02
EP1589802B1 EP1589802B1 (en) 2014-07-16

Family

ID=32822936

Family Applications (1)

Application Number Title Priority Date Filing Date
EP04706390.4A Expired - Lifetime EP1589802B1 (en) 2003-01-31 2004-01-29 Method for treating seeds, and seeds obtainable with the method

Country Status (5)

Country Link
US (1) US7748163B2 (en)
EP (1) EP1589802B1 (en)
CN (1) CN100376130C (en)
NL (1) NL1022549C2 (en)
WO (1) WO2004066710A1 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110547191A (en) * 2019-08-28 2019-12-10 铜仁万山九丰现代农业科技有限公司 Seed cultivation method for improving salt tolerance of common head cabbage

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101808540B (en) * 2007-07-02 2013-04-10 布勒巴特公司 Process for surface pasteurization and sterilization of food pieces
DK3107366T3 (en) * 2014-02-21 2020-09-21 Thermoseed Global Ab PRIMING SEEDS
JP6782882B2 (en) * 2014-11-13 2020-11-11 国立研究開発法人農業・食品産業技術総合研究機構 Seed disinfectant
WO2016198644A1 (en) * 2015-06-12 2016-12-15 Incotec Holding B.V. Seed disinfection method
FR3053566B1 (en) 2016-07-07 2019-07-12 In-Between Sprl PROCESS FOR HARVESTING AND TREATING WATER JACINTHS
CN107155448A (en) * 2017-05-20 2017-09-15 蚌埠市金牛湾农业科技发展有限公司 A kind of germination accelerating method of rose seed
CN109122257A (en) * 2018-08-10 2019-01-04 安徽省三分地农业发展有限公司 A method of promoting the plantation of clover shoot vegetable nutrient content
US11746238B1 (en) 2022-03-22 2023-09-05 In-Between International S. A. Water hyacinth fiber-based materials

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4911889A (en) * 1987-09-16 1990-03-27 The Procter & Gamble Company Methods for sanitizing materials utilizing superheated steam
GB2221830B (en) * 1988-07-28 1992-08-26 Daniel Henry Dudek Means and method for sterilizing spices and like substances
AU3188693A (en) * 1992-02-07 1993-08-12 Mccormick & Company, Inc. Method and apparatus for continuous sterilization and drying of spices and leafy herbs
JP3225474B2 (en) * 1993-03-13 2001-11-05 株式会社大生機械 Sterilization method of vegetable seeds of sprouts and cultivation method of vegetable sprouts
EP0755190A1 (en) 1994-12-29 1997-01-29 Burns Philp Food Inc. Method and apparatus for sterilization of seeds, herbs and spices
DE19605650A1 (en) * 1995-12-19 1997-06-26 Wolfgang Prof Dr Luecke Treatment or stressing of biological substances with microwaves in presence of water vapour
US6066351A (en) * 1998-03-09 2000-05-23 Tabata, Inc. Method for treating raw foods
JP4426663B2 (en) * 1999-01-26 2010-03-03 株式会社大生機械 Sterilization of alfalfa seed
DE10203190A1 (en) * 2002-01-25 2003-08-07 Buehler Ag Process to sterilize natural products such as seeds, dried fruit, root crops and cocoa beans in steam reactor

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See references of WO2004066710A1 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110547191A (en) * 2019-08-28 2019-12-10 铜仁万山九丰现代农业科技有限公司 Seed cultivation method for improving salt tolerance of common head cabbage
CN110547191B (en) * 2019-08-28 2021-03-09 铜仁万山九丰现代农业科技有限公司 Seed cultivation method for improving salt tolerance of common head cabbage

Also Published As

Publication number Publication date
CN100376130C (en) 2008-03-26
US7748163B2 (en) 2010-07-06
CN1744812A (en) 2006-03-08
NL1022549C2 (en) 2004-08-03
WO2004066710A1 (en) 2004-08-12
US20060150493A1 (en) 2006-07-13
EP1589802B1 (en) 2014-07-16

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