CN107155448A - A kind of germination accelerating method of rose seed - Google Patents
A kind of germination accelerating method of rose seed Download PDFInfo
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- CN107155448A CN107155448A CN201710360033.0A CN201710360033A CN107155448A CN 107155448 A CN107155448 A CN 107155448A CN 201710360033 A CN201710360033 A CN 201710360033A CN 107155448 A CN107155448 A CN 107155448A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/36—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids
- A01N37/38—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids having at least one oxygen or sulfur atom attached to an aromatic ring system
- A01N37/40—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids having at least one oxygen or sulfur atom attached to an aromatic ring system having at least one carboxylic group or a thio analogue, or a derivative thereof, and one oxygen or sulfur atom attached to the same aromatic ring system
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
- A01N37/46—N-acyl derivatives
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/12—Asteraceae or Compositae [Aster or Sunflower family], e.g. daisy, pyrethrum, artichoke, lettuce, sunflower, wormwood or tarragon
Abstract
The invention mainly relates to planting technology field, a kind of germination accelerating method of rose seed is disclosed, including：Seed immersion, seed enzymolysis, seed drying, presprouting of seeds；Method is simple, is easy to operation, the germination time of seed is shorten to 5 ~ 6 days, germinates within after planting 8 ~ 10 days, germination percentage improves 19.4% compared with comparative example, hence it is evident that improve plantation efficiency, planting cost is saved 12.7%；Rose seed is placed in cryopreservation in refrigerator, the germinating capacity of seed is preserved, is soaked when seed is placed in into long in urea liquid, also contain salt compounded of iodine and sodium selenite in urea liquid, using the teaching of the invention it is possible to provide nutritional ingredient, accelerate kind of soft and soggyization, promote nutritional ingredient to enter seed, improve the germinating capacity of seed；Soak addition complex enzyme in backward seed to be digested, destroy seed coat structure, accelerate nutritional ingredient and moisture enters Interior Seed, promote germination, shorten germinating time, raising rose percentage of seedgermination.
Description
Technical field
The invention mainly relates to planting technology field, more particularly to a kind of germination accelerating method of rose seed.
Background technology
Rose, is one of ten big famous flowers of Chinese tradition, is also one of big cut-flower in the world four, have the U.S. of " spending middle queen "
Claim, florescence rose can secrete phytocide, kill pathogen in air, beneficial to healthy, the rose flower just opened and
Root can be used as medicine, and have qi-regulating, promoting blood circulation, Thyme Extract, cure mainly irregular menstruation, traumatic injury, irritability stomachache, newborn too fat to move pain is alleviated
Fatigue, beauty and skin care, anti-aging, fructus rosae can be made into jam, with flavour, and fruit contains abundant vitamin, can
Prevent acute and chronic infectious disease, coronary heart disease, hepatopathy, anticancer suppression cancer.
The propagation method of rose mainly has offshoot, grafting, cuttage and seminal propagation method, and seminal propagation can be cultivated
New varieties, and method is simple, and during seed maturity, harvesting in time is sowed or by seed sand storage to Second Year spring sowing kind, but is planted
Son after planting needs could germinate for 4 ~ 5 months, or after vernalization 3 ~ 4 months, could germinate within after planting 20 ~ 30 days, and germinates
Rate can only achieve 60% or so, and the breeding cycle is longer, hence it is evident that improve planting cost, accordingly, it would be desirable to which a kind of accelerate rose seed hair
The germination accelerating method of bud.
The content of the invention
In order to make up the defect of prior art, it is an object of the invention to provide a kind of germination accelerating method of rose seed.
A kind of germination accelerating method of rose seed, comprises the following steps：
（1）Seed soaks：In the refrigerator that 1 ~ 4 DEG C is placed in after rose seed collection, the germinating capacity of seed is preserved, will before sowing
Rose seed is placed in the urea liquid that mass concentration is 1.2 ~ 1.4%, is standing and soak in 24 ~ 26 DEG C 10 ~ 12 hours, urea is molten
Also contain salt compounded of iodine and sodium selenite in liquid, using the teaching of the invention it is possible to provide nutritional ingredient, accelerate kind of soft and soggyization, promote nutritional ingredient to enter seed,
The germinating capacity of seed is improved, filtering, clear water is rinsed, and obtains immersion seed；
（2）Seed is digested：Seed will be soaked to be placed in the water of 40 ~ 50 times of amounts of seed weight, with 0.3 ~ 0.4 DEG C/min of speed
Be gradually heated to 40 ~ 42 DEG C, add complex enzyme, insulated and stirred is digested 160 ~ 180 minutes, destroys seed coat structure, accelerate nutrition into
Divide and moisture enters Interior Seed, promote germination, shorten germinating time, improve rose percentage of seedgermination, obtain enzymolysis seed；
（3）Seed drying：Enzymolysis seed is placed in freeze drier, it is 17 ~ 19% to be freeze-dried in -42 ~ -40 DEG C to water content,
Intraseminal moisture, enhancing seed resistance and adaptive capacity to environment are reduced, increase kind skin porous accelerates seed pair during vernalization
Nutrition and the absorption of moisture, shorten germination time, obtain dry seed；
（4）Presprouting of seeds：Dry seed is placed in germinating box, fountain solution is sprayed, normal temperature infiltrates 6 ~ 8 hours, raw material in fountain solution
It is abundant, while seed moisture content is kept, using the teaching of the invention it is possible to provide abundant nutrition and antimicrobial component, increase plumule vigor, prevent and treat seed
Go rotten, improve percentage of seedgermination, adjust vernalization condition, 1 ~ 3 day, temperature be 21 ~ 22 DEG C, ambient humidity is 74 ~ 76%, dark, 4
It and after, temperature is 23 ~ 24 DEG C, ambient humidity is that 71 ~ 73%, illumination is 80 ~ 100lx, is showed money or valuables one carries unintentionally to seed, first low temperature and high relative humidity,
Keep dark, nutrition and the accumulation of moisture in increase seed strengthen seed resistance, then temperature is improved, and increase illumination, stimulate
Plumule grows, and accelerates germination, shortens the germinating time of seed, obtains vernalization seed.
The step（1）Rose seed, for the seed in after harvesting 3 years.
The step（1）Urea liquid, also containing the salt compounded of iodine and mass concentration that mass concentration is 1.1 ~ 1.3% be 0.2 ~
0.4% sodium selenite.
The step（2）Complex enzyme, be made up of the raw material of following parts by weight：Cellulase 15 ~ 17, lignoenzyme 12 ~
14th, lipase 6 ~ 8, pectase 3 ~ 5.
The step（4）Fountain solution, be made up of the raw material of following parts by weight：It is glycerine 82 ~ 84, methyl hydroxybenzoate 11 ~ 13, sweet
Oxalic acid dipotassium 14 ~ 16, gamma-polyglutamic acid 7 ~ 9, Beggarticks extract 2.1 ~ 2.3, Herba Agerati Conyzoidis extract 1.5 ~ 1.7, by oxybenzene first
Ester is added in glycerine, and stirring obtains glycerite, dipotassium glycyrrhizinate and gamma-polyglutamic acid are added to the water to well mixed, is mixed
Uniformly, the aqueous solution is obtained, glycerite and the aqueous solution are well mixed, remaining raw material is added, is well mixed, is added water, make glycerine
Final volume fraction be 1.7 ~ 1.9%, obtain fountain solution.
Described Beggarticks extract, is to crush beggar-ticks, and the mass concentration for adding 30 ~ 40 times of amounts of beggar-ticks weight is
1.2 ~ 1.4% edible alkali solution, is soaked 2 ~ 3 hours in 40 ~ 50 DEG C, filtering, abandons filtrate, and filter residue is cleaned with clear water, adds filter residue
The alcoholic solution that the volume fraction of 80 ~ 90 times of amounts of weight is 67 ~ 69%, backflow extraction, it is 7 ~ 9% that filtrate, which is dried to water content,
Obtain Beggarticks extract.
Described Herba Agerati Conyzoidis extract, is to crush Ageratum conyzoides, adds the water of 90 ~ 100 times of amounts of Ageratum conyzoides weight, in 46 ~
48 DEG C, 27 ~ 29kHz ultrasounds 25 ~ 30 minutes, filtering, abandon filter residue, and absolute alcohol is slowly added into filtrate, and normal temperature stands 12 ~ 14
Hour, filtering, except precipitation, it is 8 ~ 10% that filtrate, which is dried to water content, obtains Herba Agerati Conyzoidis extract.
The step（4）Ambient humidity, with sprinkling fountain solution keep.
It is an advantage of the invention that：A kind of germination accelerating method for rose seed that the present invention is provided, method is simple, is easy to operation,
The germination time of seed is shorten to 5 ~ 6 days, germinate within after planting 8 ~ 10 days, germination percentage improves 19.4% compared with comparative example, hence it is evident that improve
Plantation efficiency, makes planting cost save 12.7%；Rose seed is placed in cryopreservation in refrigerator, the germinating capacity of seed is preserved,
Soaked when seed is placed in into long in urea liquid, salt compounded of iodine and sodium selenite also contained in urea liquid, using the teaching of the invention it is possible to provide nutritional ingredient,
Accelerate kind of soft and soggyization, promote nutritional ingredient to enter seed, improve the germinating capacity of seed；Soak in backward seed and add complex enzyme
Digested, destroy seed coat structure, accelerated nutritional ingredient and moisture enters Interior Seed, promote germination, when shortening germination
Between, improve rose percentage of seedgermination；Seed after enzymolysis is freeze-dried, intraseminal moisture is reduced, enhancing seed resists
Inverse property and adaptive capacity to environment, increase kind skin porous, seed is to nutrition and the absorption of moisture during quickening vernalization, when shortening vernalization
Between；Abundant raw material in seed, fountain solution first is infiltrated with fountain solution before vernalization, while seed moisture content is kept, using the teaching of the invention it is possible to provide rich
Rich nutrition and antimicrobial component, increase plumule vigor, and preventing and treating seed goes rotten, and improves percentage of seedgermination, gradually changes after infiltration and urges
Budling part, first low temperature and high relative humidity keeps dark, nutrition and the accumulation of moisture in increase seed, strengthens seed resistance, then by temperature
Improve, increase illumination, stimulate plumule to grow, accelerate germination, shorten the germinating time of seed.
Embodiment
Illustrate the present invention with specific embodiment below.
Embodiment 1
A kind of germination accelerating method of rose seed, comprises the following steps：
（1）Seed soaks：In the refrigerator that 1 DEG C is placed in after rose seed collection, the germinating capacity of seed is preserved, before sowing, by rose
Rare seed is placed in the urea liquid that mass concentration is 1.2%, is standing and soak for 10 hours, is also contained in urea liquid in 24 ~ 26 DEG C
Salt compounded of iodine and sodium selenite, using the teaching of the invention it is possible to provide nutritional ingredient, accelerate kind of soft and soggyization, promote nutritional ingredient to enter seed, improve seed
Germinating capacity, filtering, clear water is rinsed, and obtains immersion seed；
（2）Seed is digested：Seed will be soaked to be placed in the water of 40 times of amounts of seed weight, be gradually heated up with 0.3 DEG C/min of speed
To 40 ~ 42 DEG C, complex enzyme is added, insulated and stirred is digested 160 minutes, destroys seed coat structure, accelerate nutritional ingredient and moisture enters
Interior Seed, promotes germination, shortens germinating time, improves rose percentage of seedgermination, obtains enzymolysis seed；
（3）Seed drying：Enzymolysis seed is placed in freeze drier, is 17 ~ 19% in -42 DEG C of freeze-dryings to water content, reduces
Intraseminal moisture, enhancing seed resistance and adaptive capacity to environment, increase kind skin porous, seed is to nutrition during quickening vernalization
With the absorption of moisture, shorten germination time, obtain dry seed；
（4）Presprouting of seeds：Dry seed is placed in germinating box, fountain solution is sprayed, normal temperature infiltrates 6 hours, and raw material is rich in fountain solution
Richness, while seed moisture content is kept, using the teaching of the invention it is possible to provide abundant nutrition and antimicrobial component, increases plumule vigor, preventing and treating seed is mould
It is rotten, improve percentage of seedgermination, adjust vernalization condition, 1 ~ 3 day, temperature was 21 DEG C, ambient humidity is 74%, dark, 4 days and after,
Temperature is 23 DEG C, ambient humidity is that 71%, illumination is 80lx, is showed money or valuables one carries unintentionally to seed, first low temperature and high relative humidity, keeps dark, in increase seed
Nutrition and the accumulation of moisture, strengthen seed resistance, then temperature is improved, and increase illumination, stimulate plumule to grow, and accelerate seed hair
Bud, shortens the germinating time of seed, obtains vernalization seed.
The step（1）Rose seed, for the seed in after harvesting 3 years.
The step（1）Urea liquid, the Asia that the salt compounded of iodine and mass concentration for being 1.1% also containing mass concentration are 0.2%
Sodium selenate.
The step（2）Complex enzyme, be made up of the raw material of following parts by weight：Cellulase 15, lignoenzyme 12, fat
Enzyme 6, pectase 3.
The step（4）Fountain solution, be made up of the raw material of following parts by weight：Glycerine 82, methyl hydroxybenzoate 11, glycyrrhizic acid two
Potassium 14, gamma-polyglutamic acid 7, Beggarticks extract 2.1, Herba Agerati Conyzoidis extract 1.5, methyl hydroxybenzoate is added in glycerine, and stirring is extremely
It is well mixed, glycerite is obtained, dipotassium glycyrrhizinate and gamma-polyglutamic acid are added to the water, is well mixed, obtains the aqueous solution, will be sweet
Oil solution and the aqueous solution are well mixed, add remaining raw material, are well mixed, add water, the final volume fraction for making glycerine is
1.7%, obtain fountain solution.
Described Beggarticks extract, is to crush beggar-ticks, and the mass concentration for adding 30 times of amounts of beggar-ticks weight is
1.2% edible alkali solution, is soaked 2 hours in 40 DEG C, filtering, abandons filtrate, and filter residue is cleaned with clear water, adds 80 times of filter residue weight
The volume fraction of amount is 67% alcoholic solution, and backflow is extracted, and it is 7 ~ 9% that filtrate, which is dried to water content, obtains Beggarticks extract.
Described Herba Agerati Conyzoidis extract, is to crush Ageratum conyzoides, adds the water of 90 times of amounts of Ageratum conyzoides weight, in 46 DEG C,
Filter residue is abandoned in 27kHz ultrasounds 25 minutes, filtering, and absolute alcohol is slowly added into filtrate, and normal temperature stands 12 hours, filtering, except heavy
Form sediment, it is 8 ~ 10% that filtrate, which is dried to water content, obtains Herba Agerati Conyzoidis extract.
The step（4）Ambient humidity, with sprinkling fountain solution keep.
Embodiment 2
A kind of germination accelerating method of rose seed, comprises the following steps：
（1）Seed soaks：In the refrigerator that 2 DEG C are placed in after rose seed collection, the germinating capacity of seed is preserved, before sowing, by rose
Rare seed is placed in the urea liquid that mass concentration is 1.3%, is standing and soak for 11 hours, is also contained in urea liquid in 24 ~ 26 DEG C
Salt compounded of iodine and sodium selenite, using the teaching of the invention it is possible to provide nutritional ingredient, accelerate kind of soft and soggyization, promote nutritional ingredient to enter seed, improve seed
Germinating capacity, filtering, clear water is rinsed, and obtains immersion seed；
（2）Seed is digested：Seed will be soaked to be placed in the water of 45 times of amounts of seed weight, be gradually heated up with 0.3 DEG C/min of speed
To 40 ~ 42 DEG C, complex enzyme is added, insulated and stirred is digested 170 minutes, destroys seed coat structure, accelerate nutritional ingredient and moisture enters
Interior Seed, promotes germination, shortens germinating time, improves rose percentage of seedgermination, obtains enzymolysis seed；
（3）Seed drying：Enzymolysis seed is placed in freeze drier, is 17 ~ 19% in -41 DEG C of freeze-dryings to water content, reduces
Intraseminal moisture, enhancing seed resistance and adaptive capacity to environment, increase kind skin porous, seed is to nutrition during quickening vernalization
With the absorption of moisture, shorten germination time, obtain dry seed；
（4）Presprouting of seeds：Dry seed is placed in germinating box, fountain solution is sprayed, normal temperature infiltrates 7 hours, and raw material is rich in fountain solution
Richness, while seed moisture content is kept, using the teaching of the invention it is possible to provide abundant nutrition and antimicrobial component, increases plumule vigor, preventing and treating seed is mould
It is rotten, improve percentage of seedgermination, adjust vernalization condition, 1 ~ 3 day, temperature was 21.5 DEG C, ambient humidity is 75%, dark, 4 days and with
Afterwards, temperature be 23.5 DEG C, ambient humidity be that 72%, illumination is 90lx, showed money or valuables one carries unintentionally to seed, first low temperature and high relative humidity keeps dark, increase
Nutrition and the accumulation of moisture in seed, strengthen seed resistance, then temperature is improved, and increase illumination, stimulate plumule to grow, and accelerate
Germination, shortens the germinating time of seed, obtains vernalization seed.
The step（1）Rose seed, for the seed in after harvesting 3 years.
The step（1）Urea liquid, the Asia that the salt compounded of iodine and mass concentration for being 1.2% also containing mass concentration are 0.3%
Sodium selenate.
The step（2）Complex enzyme, be made up of the raw material of following parts by weight：Cellulase 16, lignoenzyme 13, fat
Enzyme 7, pectase 4.
The step（4）Fountain solution, be made up of the raw material of following parts by weight：Glycerine 83, methyl hydroxybenzoate 12, glycyrrhizic acid two
Potassium 15, gamma-polyglutamic acid 8, Beggarticks extract 2./2, Herba Agerati Conyzoidis extract 1.6, methyl hydroxybenzoate is added in glycerine, stirring
To well mixed, glycerite is obtained, dipotassium glycyrrhizinate and gamma-polyglutamic acid are added to the water, be well mixed, obtain the aqueous solution, will
Glycerite and the aqueous solution are well mixed, add remaining raw material, are well mixed, add water, the final volume fraction for making glycerine is
1.7 ~ 1.9%, obtain fountain solution.
Described Beggarticks extract, is to crush beggar-ticks, and the mass concentration for adding 35 times of amounts of beggar-ticks weight is
1.3% edible alkali solution, is soaked 2 hours in 45 DEG C, filtering, abandons filtrate, and filter residue is cleaned with clear water, adds 85 times of filter residue weight
The volume fraction of amount is 68% alcoholic solution, and backflow is extracted, and it is 7 ~ 9% that filtrate, which is dried to water content, obtains Beggarticks extract.
Described Herba Agerati Conyzoidis extract, is to crush Ageratum conyzoides, adds the water of 95 times of amounts of Ageratum conyzoides weight, in 47 DEG C,
Filter residue is abandoned in 28kHz ultrasounds 28 minutes, filtering, and absolute alcohol is slowly added into filtrate, and normal temperature stands 13 hours, filtering, except heavy
Form sediment, it is 8 ~ 10% that filtrate, which is dried to water content, obtains Herba Agerati Conyzoidis extract.
The step（4）Ambient humidity, with sprinkling fountain solution keep.
Embodiment 3
A kind of germination accelerating method of rose seed, comprises the following steps：
（1）Seed soaks：In the refrigerator that 4 DEG C are placed in after rose seed collection, the germinating capacity of seed is preserved, before sowing, by rose
Rare seed is placed in the urea liquid that mass concentration is 1.4%, is standing and soak for 12 hours, is also contained in urea liquid in 24 ~ 26 DEG C
Salt compounded of iodine and sodium selenite, using the teaching of the invention it is possible to provide nutritional ingredient, accelerate kind of soft and soggyization, promote nutritional ingredient to enter seed, improve seed
Germinating capacity, filtering, clear water is rinsed, and obtains immersion seed；
（2）Seed is digested：Seed will be soaked to be placed in the water of 50 times of amounts of seed weight, be gradually heated up with 0.4 DEG C/min of speed
To 40 ~ 42 DEG C, complex enzyme is added, insulated and stirred is digested 180 minutes, destroys seed coat structure, accelerate nutritional ingredient and moisture enters
Interior Seed, promotes germination, shortens germinating time, improves rose percentage of seedgermination, obtains enzymolysis seed；
（3）Seed drying：Enzymolysis seed is placed in freeze drier, is 19% in -40 DEG C of freeze-dryings to water content, reduces and plant
Moisture in son, enhancing seed resistance and adaptive capacity to environment, an increase kind skin porous, accelerate during vernalization seed to nutrition and
The absorption of moisture, shortens germination time, obtains dry seed；
（4）Presprouting of seeds：Dry seed is placed in germinating box, fountain solution is sprayed, normal temperature infiltrates 8 hours, and raw material is rich in fountain solution
Richness, while seed moisture content is kept, using the teaching of the invention it is possible to provide abundant nutrition and antimicrobial component, increases plumule vigor, preventing and treating seed is mould
It is rotten, improve percentage of seedgermination, adjust vernalization condition, 1 ~ 3 day, temperature was 22 DEG C, ambient humidity is 76%, dark, 4 days and after,
Temperature is 24 DEG C, ambient humidity is that 73%, illumination is 100lx, is showed money or valuables one carries unintentionally to seed, first low temperature and high relative humidity, keeps dark, increases seed
Interior nutrition and the accumulation of moisture, strengthen seed resistance, then temperature is improved, and increase illumination, stimulate plumule to grow, and accelerate seed
Germination, shortens the germinating time of seed, obtains vernalization seed.
The step（1）Rose seed, for the seed in after harvesting 3 years.
The step（1）Urea liquid, the Asia that the salt compounded of iodine and mass concentration for being 1.3% also containing mass concentration are 0.4%
Sodium selenate.
The step（2）Complex enzyme, be made up of the raw material of following parts by weight：Cellulase 17, lignoenzyme 14, fat
Enzyme 8, pectase 5.
The step（4）Fountain solution, be made up of the raw material of following parts by weight：Glycerine 84, methyl hydroxybenzoate 13, glycyrrhizic acid two
Potassium 16, gamma-polyglutamic acid 9, Beggarticks extract 2.3, Herba Agerati Conyzoidis extract 1.7, methyl hydroxybenzoate is added in glycerine, and stirring is extremely
It is well mixed, glycerite is obtained, dipotassium glycyrrhizinate and gamma-polyglutamic acid are added to the water, is well mixed, obtains the aqueous solution, will be sweet
Oil solution and the aqueous solution are well mixed, add remaining raw material, are well mixed, add water, and the final volume fraction for making glycerine is 1.7
~ 1.9%, obtain fountain solution.
Described Beggarticks extract, is to crush beggar-ticks, and the mass concentration for adding 40 times of amounts of beggar-ticks weight is
1.4% edible alkali solution, is soaked 3 hours in 50 DEG C, filtering, abandons filtrate, and filter residue is cleaned with clear water, adds 90 times of filter residue weight
The volume fraction of amount is 69% alcoholic solution, and backflow is extracted, and it is 7 ~ 9% that filtrate, which is dried to water content, obtains Beggarticks extract.
Described Herba Agerati Conyzoidis extract, is to crush Ageratum conyzoides, adds the water of 100 times of amounts of Ageratum conyzoides weight, in 48 DEG C,
Filter residue is abandoned in 29kHz ultrasounds 30 minutes, filtering, and absolute alcohol is slowly added into filtrate, and normal temperature stands 14 hours, filtering, except heavy
Form sediment, it is 8 ~ 10% that filtrate, which is dried to water content, obtains Herba Agerati Conyzoidis extract.
The step（4）Ambient humidity, with sprinkling fountain solution keep.
Comparative example 1
Removal step（1）In harvesting after be placed in 4 DEG C of refrigerator, remaining method, be the same as Example 1.
Comparative example 2
Step（1）In urea liquid be changed to water, remaining method, be the same as Example 1.
Comparative example 3
Removal step（2）, remaining method, be the same as Example 1.
Comparative example 4
Removal step（3）, remaining method, be the same as Example 1.
Comparative example 5
Removal step（4）In fountain solution, remaining method, be the same as Example 1.
Comparative example 6
Step（4）In vernalization condition keeping temperature be 21 DEG C, ambient humidity be 74%, dark, remaining method, be the same as Example 1.
Comparative example 7
The buried germination accelerating method of existing traditional rose seed.
The Germination effect of embodiment and comparative example rose seed accelerating germination method：
In Anhui Province Bengbu Region, 10000, red rose seed that is ripe, full, displaying 3 years is selected, 10 groups are randomly divided into, often
Group 1000, respectively embodiment group and comparative example group, each group carry out vernalization processing, vernalization according to the germination accelerating method of the group respectively
The seed number that shows money or valuables one carries unintentionally is observed after 6 days, vernalization observation germination after drilling is carried out in the soil put in order, sowing 10 days, which is unearthed, to be counted,
The Germination effect of other management method all sames, embodiment and comparative example rose seed accelerating germination method is shown in Table 1.
Table 1：The Germination effect of embodiment and comparative example rose seed accelerating germination method
| Project | Seed number（Grain） | Show money or valuables one carries unintentionally number（Grain） | Germinative number（Strain） | Germination percentage（%） |
| Embodiment 1 | 1000 | 426 | 572 | 57.2 |
| Embodiment 2 | 1000 | 453 | 595 | 59.5 |
| Embodiment 3 | 1000 | 437 | 584 | 58.4 |
| Comparative example 1 | 1000 | 216 | 417 | 41.7 |
| Comparative example 2 | 1000 | 385 | 484 | 48.4 |
| Comparative example 3 | 1000 | 353 | 478 | 47.8 |
| Comparative example 4 | 1000 | 311 | 464 | 46.4 |
| Comparative example 5 | 1000 | 352 | 503 | 50.3 |
| Comparative example 6 | 1000 | 407 | 495 | 49.5 |
| Comparative example 7 | 1000 | 13 | 401 | 40.1 |
Show from the result of table 1, the germination accelerating method of the rose seed of embodiment, the rose seed of 3 years is displayed in sowing, save plantation
Resource, vernalization and germination unearthed time are short, and germination percentage is substantially high compared with comparative example, illustrate urging for the rose seed that the present invention is provided
Bud method has good Germination effect.
Claims (8)
1. a kind of germination accelerating method of rose seed, it is characterised in that comprise the following steps：
（1）Seed soaks：In the refrigerator that 1 ~ 4 DEG C is placed in after rose seed collection, before sowing, rose seed is placed in mass concentration
In 1.2 ~ 1.4% urea liquid, to be standing and soak in 24 ~ 26 DEG C 10 ~ 12 hours, filtering, clear water is rinsed, and obtains immersion seed；
（2）Seed is digested：Seed will be soaked to be placed in the water of 40 ~ 50 times of amounts of seed weight, with 0.3 ~ 0.4 DEG C/min of speed
40 ~ 42 DEG C are gradually heated to, complex enzyme is added, insulated and stirred is digested 160 ~ 180 minutes, obtains enzymolysis seed；
（3）Seed drying：Enzymolysis seed is placed in freeze drier, it is 17 ~ 19% to be freeze-dried in -42 ~ -40 DEG C to water content,
Obtain dry seed；
（4）Presprouting of seeds：Dry seed is placed in germinating box, fountain solution is sprayed, normal temperature infiltrates 6 ~ 8 hours, adjusts vernalization condition,
1 ~ 3 day, temperature was 21 ~ 22 DEG C, ambient humidity is 74 ~ 76%, dark, 4 days and after, temperature is 23 ~ 24 DEG C, ambient humidity is
71 ~ 73%, illumination is 80 ~ 100lx, is showed money or valuables one carries unintentionally to seed, obtains vernalization seed.
2. the germination accelerating method of rose seed according to claim 1, it is characterised in that the step（1）Rose seed, be
Seed after harvesting in 3 years.
3. the germination accelerating method of rose seed according to claim 1, it is characterised in that the step（1）Urea liquid, also
Contain the salt compounded of iodine that mass concentration is 1.1 ~ 1.3% and sodium selenite that mass concentration is 0.2 ~ 0.4%.
4. the germination accelerating method of rose seed according to claim 1, it is characterised in that the step（2）Complex enzyme, by with
The raw material composition of lower parts by weight：Cellulase 15 ~ 17, lignoenzyme 12 ~ 14, lipase 6 ~ 8, pectase 3 ~ 5.
5. the germination accelerating method of rose seed according to claim 1, it is characterised in that the step（4）Fountain solution, by with
The raw material composition of lower parts by weight：Glycerine 82 ~ 84, methyl hydroxybenzoate 11 ~ 13, dipotassium glycyrrhizinate 14 ~ 16, gamma-polyglutamic acid 7 ~ 9, ghost
Needle grass extract 2.1 ~ 2.3, Herba Agerati Conyzoidis extract 1.5 ~ 1.7, methyl hydroxybenzoate is added in glycerine, and stirring is obtained to well mixed
Glycerite, dipotassium glycyrrhizinate and gamma-polyglutamic acid are added to the water, and are well mixed, are obtained the aqueous solution, by glycerite and water-soluble
Liquid is well mixed, and adds remaining raw material, is well mixed, is added water, and the final volume fraction for making glycerine is 1.7 ~ 1.9%, must be soaked
Liquid.
6. the germination accelerating method of rose seed according to claim 5, it is characterised in that described Beggarticks extract, be by
Beggar-ticks is crushed, and the mass concentration for adding 30 ~ 40 times of amounts of beggar-ticks weight is 1.2 ~ 1.4% edible alkali solution, in 40 ~ 50 DEG C
Immersion 2 ~ 3 hours, filtering, abandons filtrate, and filter residue is cleaned with clear water, add the volume fraction of 80 ~ 90 times of amounts of filter residue weight for 67 ~
69% alcoholic solution, backflow extraction, it is 7 ~ 9% that filtrate, which is dried to water content, obtains Beggarticks extract.
7. the germination accelerating method of rose seed according to claim 5, it is characterised in that described Herba Agerati Conyzoidis extract, be by
Ageratum conyzoides is crushed, and adds the water of 90 ~ 100 times of amounts of Ageratum conyzoides weight, in 46 ~ 48 DEG C, 27 ~ 29kHz ultrasounds 25 ~ 30 minutes, filtering,
Filter residue is abandoned, absolute alcohol is slowly added into filtrate, normal temperature stands 12 ~ 14 hours, except precipitation, filtrate is dried to containing for filtering
Water is 8 ~ 10%, obtains Herba Agerati Conyzoidis extract.
8. the germination accelerating method of rose seed according to claim 1, it is characterised in that the step（4）Ambient humidity, use
Fountain solution is sprayed to keep.
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| CN108966727A (en) * | 2018-06-27 | 2018-12-11 | 安徽省万果园农业科技发展有限公司 | A kind of method for treating seeds improving rose quality |
| CN108966736A (en) * | 2018-07-20 | 2018-12-11 | 山东颐科环境科技有限公司 | A method of releasing the suspend mode when administering salt-soda soil of wild rose seed |
| CN109362270A (en) * | 2018-08-31 | 2019-02-22 | 句容市丁庄葡萄研究所 | A method of improving grape seed germination rate |
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