CN110501514A - Automatic tester and automatic checkout system - Google Patents
Automatic tester and automatic checkout system Download PDFInfo
- Publication number
- CN110501514A CN110501514A CN201910892663.1A CN201910892663A CN110501514A CN 110501514 A CN110501514 A CN 110501514A CN 201910892663 A CN201910892663 A CN 201910892663A CN 110501514 A CN110501514 A CN 110501514A
- Authority
- CN
- China
- Prior art keywords
- platform
- micro
- driving
- fluidic chip
- incubated
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000001514 detection method Methods 0.000 claims abstract description 151
- 238000011534 incubation Methods 0.000 claims abstract description 83
- 238000004140 cleaning Methods 0.000 claims abstract description 61
- 210000003934 vacuole Anatomy 0.000 claims abstract description 33
- 238000006073 displacement reaction Methods 0.000 claims abstract description 21
- 239000000243 solution Substances 0.000 claims description 33
- 239000012530 fluid Substances 0.000 claims description 29
- 238000002347 injection Methods 0.000 claims description 13
- 239000007924 injection Substances 0.000 claims description 13
- 239000002699 waste material Substances 0.000 claims description 12
- 239000003153 chemical reaction reagent Substances 0.000 claims description 11
- 239000007788 liquid Substances 0.000 claims description 11
- 238000001179 sorption measurement Methods 0.000 claims description 9
- 239000011324 bead Substances 0.000 claims description 8
- 238000002156 mixing Methods 0.000 claims description 8
- 230000033001 locomotion Effects 0.000 claims description 6
- 239000000427 antigen Substances 0.000 claims description 4
- 102000036639 antigens Human genes 0.000 claims description 4
- 108091007433 antigens Proteins 0.000 claims description 4
- 238000010276 construction Methods 0.000 claims description 4
- 238000010438 heat treatment Methods 0.000 claims description 4
- 238000007689 inspection Methods 0.000 claims description 4
- 239000003550 marker Substances 0.000 claims description 4
- 238000004891 communication Methods 0.000 claims description 2
- 239000006210 lotion Substances 0.000 claims description 2
- 239000007787 solid Substances 0.000 claims description 2
- 238000005406 washing Methods 0.000 claims description 2
- 238000009826 distribution Methods 0.000 abstract description 3
- 238000006243 chemical reaction Methods 0.000 description 11
- 238000000034 method Methods 0.000 description 9
- 230000008569 process Effects 0.000 description 8
- 238000010586 diagram Methods 0.000 description 7
- 239000000758 substrate Substances 0.000 description 5
- 238000005485 electric heating Methods 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 230000000694 effects Effects 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 230000009471 action Effects 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 230000005540 biological transmission Effects 0.000 description 2
- 238000009795 derivation Methods 0.000 description 2
- 230000005611 electricity Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000006870 function Effects 0.000 description 2
- 238000003018 immunoassay Methods 0.000 description 2
- NJPPVKZQTLUDBO-UHFFFAOYSA-N novaluron Chemical compound C1=C(Cl)C(OC(F)(F)C(OC(F)(F)F)F)=CC=C1NC(=O)NC(=O)C1=C(F)C=CC=C1F NJPPVKZQTLUDBO-UHFFFAOYSA-N 0.000 description 2
- 238000011112 process operation Methods 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000002156 adsorbate Substances 0.000 description 1
- 239000005030 aluminium foil Substances 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000000428 dust Substances 0.000 description 1
- 238000001125 extrusion Methods 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 230000028993 immune response Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 238000011065 in-situ storage Methods 0.000 description 1
- 238000001746 injection moulding Methods 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000013011 mating Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000002985 plastic film Substances 0.000 description 1
- 229920006255 plastic film Polymers 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000004801 process automation Methods 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 238000004904 shortening Methods 0.000 description 1
- 230000001360 synchronised effect Effects 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
- G01N33/532—Production of labelled immunochemicals
- G01N33/533—Production of labelled immunochemicals with fluorescent label
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
- G01N33/54326—Magnetic particles
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/00584—Control arrangements for automatic analysers
Abstract
The invention discloses a kind of automatic testers, include bracket, are incubated for component and unloading detection components；Branch is provided with moving member and drives the first driving of moving member displacement；It is incubated for component and is equipped with extending direction distribution multiple, along moving member, it include that mounting base, the incubation platform for placing micro-fluidic chip and pressurizing unit, driving are incubated for platform and the second driving of pressurizing unit relative displacement, electric heater unit, when the second cleaning vacuole that driving drive is incubated for platform and pressurizing unit is close, pressurizing unit can rack micro-fluidic chip；Unloading detection components are located at the side of moving member, include and is incubated for detection platform, the clamping device, third driving and detection device for driving clamping device along the direction displacement close to or far from incubation platform that platform is in same level, when being incubated for platform close to detection platform, gripping apparatus grips micro-fluidic chip is displaced and unloads along the extending direction of detection platform.
Description
Technical field
The present invention relates to technical field of biological, automatically more specifically to a kind of automatic tester and including this
The automatic checkout system of detector.
Background technique
Micro-fluidic chip refers to and micro-channel structure and other function element is arranged on chip (as being wrapped in cleaning solution
Cleaning vacuole), so that sample such as is loaded in fluid channel, is diluted, being mixed, being reacted, being separated at the preparation manipulations.Currently, miniflow
Control chip has been applied to immune detection analysis field, is immunoassay device with its mating application.Operator adds sample
Enter in the fluid channel of micro-fluidic chip, sample, which flows in fluid channel and immune response occurs in flow process, generates combination
Object, conjugate can be attracted to some region (adsorption zone) in fluid channel, and then operator will be located at fluid channel and originate
The cleaning vacuole at end, which punctures, to be made in cleaning solution injection fluid channel, and cleaning solution is by the remaining sample wash except conjugate to adsorption zone
Outside makes a remaining conjugate in adsorption zone, and then operator can use combination of the immunoassay device to adsorption zone
Object carries out the parameters that immune detection obtains test sample.
In recent years, in order to reduce manual operation, produce full-automatic micro-fluidic detection instrument, but it is in the prior art these from
Dynamicization instrument, sample of adding an examination of automatically, centrifugal treating sample, injection cleaning solution, scrap concrete, wash the operating process such as needle at reagent adding
It is cumbersome, process is tediously long, and can not achieve the progress to multiple samples (i.e. micro-fluidic chip) while operating, can not realize quickly
Detection.
Therefore, develop a kind of operation simplify, can for much samples and meanwhile operate and can be realized quickly detect it is automatic
Detector becomes the problem of current urgent need to resolve.
Summary of the invention
The object of the present invention is to provide a kind of operations to simplify, can operate and can be realized for multiple micro-fluidic chips simultaneously
The automatic tester quickly detected.The object of the invention is also to provide a kind of automatic detection systems including above-mentioned automatic tester
System.
To achieve the above object, a kind of automatic tester provided by the invention includes bracket, for carrying micro-fluidic core
The incubation component of piece, for making the micro-fluidic chip be detached from the incubation component and being detected to the micro-fluidic chip
Unload detection components and control device；Moving member is provided on the bracket and for driving the moving member along straight line path or
First driving of closed circle path shift reciprocately；Extending direction multiple and along the moving member is arranged in the incubation component
Distribution, the incubation component include the mounting base being fixedly connected with the moving member, are connected in the mounting base for carrying
The incubation platform of the micro-fluidic chip, the pressurizing unit being connect with the mounting base, for driving the incubation platform and institute
The second driving, the electric heating on the incubation platform for being heated to the incubation platform for stating pressurizing unit relative displacement
Device, when second driving drives the incubation platform and the pressurizing unit is close, the pressurizing unit can rack institute
State the cleaning vacuole of micro-fluidic chip；The unloading detection components are located at the side of the moving member and are in the moving member
In moving range, the unloading detection components include the detection platform being in same level with the incubation platform, connect
Connect the clamping device in the detection platform, for driving the clamping device along close to or far from the side for being incubated for platform
It drives and is connected to above the detection platform to the third of displacement for detecting the detection dress of sample in the micro-fluidic chip
It sets, when the incubation platform is close to the detection platform, micro-fluidic chip described in the gripping apparatus grips is along the detection
The extending direction of platform is displaced and the micro-fluidic chip can be discharged in the detection platform；The control device and institute
It states the first driving, second driving, third driving electrical connection and is communicated to connect with the detection device.
Preferably, the incubation platform is rotatably connected in the mounting base by shaft, and the axis of the shaft
To with it is described incubation platform where plane parallel, with it is described incubation platform first end to second end direction it is perpendicular,
The shaft is connected to the position far from the first end for being incubated for platform；Second driving connects with the incubation platform
It connects and rotates the incubation platform around the shaft；The pressurizing unit is located on the rotation path for being incubated for platform,
When the incubation platform turns to first position, the first end for being incubated for platform is higher than the second end and the extruding
Device and the cleaning vacuole offset and can rack the cleaning vacuole.
It preferably, further include having described in catch bar for the micro-fluidic chip to be released to the detection platform, drive
The 4th driving and discard bin for holding the micro-fluidic chip that catch bar is moved along its axial reciprocating, the 4th driving
And the discard bin connects on the bracket and the discard bin is located on the pushing direction of the catch bar.
Preferably, the moving member is set as connecting turntable on the bracket, and first driving includes driving
The turntable surrounds the motor of center axis thereof rotation, the power output axis connection of the turntable and the motor, the unloading
Detection components are located on the rotation path for being incubated for component.
Preferably, it includes the motor being fixedly connected with the bracket that the third, which drives, defeated with the power of the motor
The lead screw of axis connection out, the clamping device are connected through a screw thread structure and are set on the lead screw, the axial direction of the lead screw with
The extending direction of the detection platform is consistent.
Preferably, the clamping device is provided with sliding shoe and is connected to the intermediate plate of the sliding shoe, on the sliding shoe
It is provided with the threaded hole protruded into for the lead screw, the intermediate plate is provided at least two, at least sets in opposite directions there are two the intermediate plate
Set and keep spacing, to form the grasping part being caught in for the micro-fluidic chip, two intermediate plates are for being stuck in institute
It states in the opposite two side walls of micro-fluidic chip；The first end of two intermediate plates is along the axially projecting in institute of the lead screw
It states sliding block, form the step structure protruded into for the end for being incubated for platform between the first end and the sliding shoe.
Preferably, the third driving is located at the lower section of the detection platform, is provided on the sliding shoe for the inspection
The through slot that platform protrudes into is surveyed, and the through slot is connected with the grasping part and is located at the lower section of the grasping part；It is described
The interception part for stopping the micro-fluidic chip, the shifting for intercepting part and being located at the grasping part are provided in detection platform
On dynamic path, and on perpendicular to the lead screw axially direction between described less than two intermediate plates of size for intercepting part
Distance.
Preferably, further include having the cabinet for covering the bracket, be provided on the cabinet for the micro-fluidic chip into
The inlet entered, the inlet are arranged on the rotation path for being incubated for component.
The present invention also provides a set of immune detection systems, include micro-fluidic chip and automatic tester, and feature exists
In the automatic tester is set as described in any item automatic testers as above.
Preferably, the micro-fluidic chip is provided with the fluid channel in enclosed construction for sample flowing, the miniflow
Road includes the sample-adding storehouse being sequentially communicated, mixing bunker, delay path, adsorption zone and waste liquid storehouse, and the sample-adding storehouse is provided with for institute
The adding mouth and the sample-adding storehouse for stating sample addition are provided with the solid state reagents ball being immunoreacted with the sample, the examination
It include the antibody/antigen for being coated with fluorescent marker and magnetic bead being immunoreacted with the sample, the suction in agent ball
The magnet for adsorbing the magnetic bead is equipped on the bottom wall in attached area；The delay path in it is multiple be arranged side by side and successively head and the tail
The capillary channel structure of connection, the waste liquid storehouse are provided with the venthole communicated with ambient atmosphere；It further include having cleaning vacuole, using
In the injection channel in the cleaning solution storehouse and the connection cleaning solution storehouse and the sample-adding storehouse that accommodate cleaning vacuole.
In technical solution provided by the invention, automatic tester includes bracket, the moving member being fixed on bracket, connection
Multiple incubation components on moving member, the unloading detection components being connected on bracket, it is micro- for carrying and being incubated for be incubated for component
Fluidic chip, unloading detection components are used to remove micro-fluidic chip and are incubated for platform and detect to it.It is also set on bracket
It is equipped with the first driving, moving member can be driven to move back and forth along straight line path or closed circle path, unloading detection components are located at
Moving member side and in moving member moving range in.When each incubation component is subjected to displacement with moving member, it is close one by one or
When contact unloading detection components, unloading detection components can detect each incubation component respectively.So set, automatic inspection
Surveying instrument can be incubated for simultaneously for multiple micro-fluidic chips, also may be implemented to multiple automatic detections for being incubated for component.
Wherein, it is incubated for the incubation platform that component is provided with the mounting base being fixedly connected with moving member, is connected in mounting base
It is used for pressurizing unit, for driving the second driving for being incubated for platform and pressurizing unit relative displacement and positioned at being incubated on platform
To the electric heater unit for being incubated for platform heating.Micro-fluidic chip is placed or is fixed on and is incubated on platform, can make to be located at miniflow
Position of the cleaning vacuole in easily close pressurizing unit on chip is controlled, when the second driving drives incubation platform and pressurizing unit to lean on
Closely, pressurizing unit can rack cleaning vacuole, be automatically injected cleaning solution in micro-fluidic chip, realize automation；And it multiple incubates
It educates component and can move at the same time, to multiple micro-fluidic chips while injecting cleaning solution to keep the consistency of test.
Unloading detection components include to be located at the detection platform of same level, for clamping micro-fluidic core with incubation platform
The clamping device of piece, the third for driving clamping device edge to be displaced close to or far from the direction for being incubated for platform drive and for examining
The detection device of sample in micrometer fluidic chip, when being incubated for platform close to detection platform, gripping apparatus grips micro-fluidic chip
It is displaced along the extending direction of detection platform and micro-fluidic chip is discharged in detection platform.At this point, being located above detection platform
Detection device can to micro-fluidic chip carry out sample detection.
And automatic tester provided by the invention further includes having control device, it can be under the automatic control of control device
Realize the automatic operating of the first driving, the second driving, third driving and detection device.So set, the realization detected automatically,
It only needs the multiple micro-fluidic chips for having been injected into sample being put into each incubation component, under the drive of the second driving, realize certainly
Dynamic injection cleaning solution makes to unload detection components one by one close to each incubation component, then exists then under the drive of the first driving
Third driving driving under, clamping device make micro-fluidic chip be detached from be incubated for component and be located at detection platform on, then it is micro-fluidic
Chip is detected by detection device.Compared with prior art, whole process operation simplifies, detects quickly.
Detailed description of the invention
In order to more clearly explain the embodiment of the invention or the technical proposal in the existing technology, to embodiment or will show below
There is attached drawing needed in technical description to be briefly described, it should be apparent that, the accompanying drawings in the following description is only this
Some embodiments of invention for those of ordinary skill in the art without creative efforts, can be with
It obtains other drawings based on these drawings.
Fig. 1 is the structural schematic diagram of automatic tester in the embodiment of the present invention；
Fig. 2 is to be incubated for component in the embodiment of the present invention and unload the unloaded state schematic diagram of detection components；
Fig. 3 is the structural schematic diagram that component is incubated in the embodiment of the present invention；
Fig. 4 is the schematic diagram that the heeling condition of platform is incubated in the embodiment of the present invention；
Fig. 5 is the connection schematic diagram of clamping device and detection platform in the embodiment of the present invention；
Fig. 6 is the structural schematic diagram of clamping device in the embodiment of the present invention；
Fig. 7 is the structural schematic diagram of micro-fluidic chip in the embodiment of the present invention；
Fig. 8 is the overall schematic of micro-fluidic chip in the embodiment of the present invention.
In Fig. 1-Fig. 8:
Micro-fluidic chip -1, bracket -2 are incubated for component -3, mounting base -301, are incubated for platform -302, extrusion -303, turn
Axis -304, the second driving -305, unloading detection components -4, detection platform -401, clamping device -402, intermediate plate -421, sliding block -
422, grasping part -423, through slot -424, step structure -425, third driving -403, lead screw -431, interception part -404, detection
Device -405, catch bar -5, turntable -6, discard bin -7, upper cover -11, substrate -12, cleaning vacuole -13, sample-adding storehouse -101, mixing
Storehouse -102, delay path -103, waste liquid storehouse -104, cleaning solution storehouse -105, injection channel -106, adding mouth -107, venthole -
108。
Specific embodiment
To make the object, technical solutions and advantages of the present invention clearer, technical solution of the present invention will be carried out below
Detailed description.Obviously, described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.Base
Embodiment in the present invention, those of ordinary skill in the art are obtained all without making creative work
Other embodiment belongs to the range that the present invention is protected.
A kind of operation of being designed to provide of present embodiment simplifies, can operate simultaneously for multiple micro-fluidic chips
And it can be realized the automatic tester quickly detected.It includes above-mentioned automatic that the purpose of present embodiment, which is also resided in and provided a kind of,
The automatic checkout system of detector.
Hereinafter, elaborating in conjunction with attached drawing to embodiment.In addition, embodiments illustrated below is not remembered claim
The content of the invention of load plays any restriction effect.In addition, the full content of composition represented by following example is not limited to conduct
Necessary to the solution of the invention described in the claims.
Please refer to attached drawing 1-8, a kind of automatic tester provided in this embodiment comprising have bracket 2, be fixed on bracket 2
On moving member, the multiple incubation components 3 being connected on moving member, the unloading detection components 4 that are connected on bracket 2, incubation group
For part 3 for carrying and being incubated for micro-fluidic chip 1, unloading detection components 4, which are used to remove micro-fluidic chip 1, is incubated for platform 302
And it is detected.It is additionally provided with the first driving on bracket 2, moving member can be driven along straight line path or closed circle path
It moves back and forth, multiple incubation components 3 connect with moving member and along the distribution of the extending direction of moving member, unloading detection components 4
On in side in moving member and the moving range in moving member.First driving is subjected to displacement incubation component 3 with moving member,
Each incubation component 3 is set to come close to or in contact with unloading detection components 4 one by one, at this point, unloading detection components 4 can be incubated to each
Component 3 is educated to be detected respectively.So set, automatic tester can be incubated for or real simultaneously for multiple micro-fluidic chips 1
Now to multiple automatic detections for being incubated for component 3；And it is incubated for component 3 and unloading detection components 4 can be fast with relative displacement, detection
Fast ground carries out one by one, and detection operation is convenient, process is smooth, may be implemented quickly to detect.
Wherein, each component 3 that is incubated for includes mounting base 301 for being fixedly connected with moving member, is connected to installation
Incubation platform 302 and pressurizing unit 303 on seat 301, second for driving incubation platform and 303 relative displacement of pressurizing unit
Driving 305 and the electric heater unit on incubation platform 302 for being heated to incubation platform 302.Sample will be had been injected into
Micro-fluidic chip 1, which is placed or is fixed on, to be incubated on platform 302, the cleaning vacuole 13 being located on micro-fluidic chip 1 can be made to be in
Easily close to the position of pressurizing unit 303, electric heater unit can provide temperature for micro-fluidic chip 1 and be incubated for, make micro-fluidic chip 1
Interior sample can be chemically reacted sufficiently.When reaction is completed, the second driving 305 drives and is incubated for platform and pressurizing unit 303
Relative displacement keeps the two close, and pressurizing unit 303 can rack cleaning vacuole 13, cleaning solution is made to be automatically injected micro-fluidic chip 1
It is interior, realize automatic operation；And multiple incubation components 3 can move at the same time, to multiple micro-fluidic chips 1 while inject cleaning solution
To keep the consistency of test.Second driving 305, which can be, is incubated for 302 direction of platform with the transmission connection of incubation platform 302, driving
Pressurizing unit 303 is displaced, and is also possible to be sequentially connected with pressurizing unit 303, is driven pressurizing unit 303 to incubation 302, platform
It moves.
Unloading detection components 4 are located in the side of moving member and the moving range in moving member in order to which the first driving makes
Each incubation component 3 is close one by one with it, and unloading detection components includes to be located at the detection of same level with incubation platform 302
Platform 401, the clamping device 402 for clamping micro-fluidic chip 1, for driving clamping device 402 along close to or far from incubation
The third driving 403 of the direction displacement of platform 302 and the detection device 405 for detecting sample in micro-fluidic chip 1.
Under the action of the first driving, when incubation platform 302 and detection platform 401 come close to or in contact with, clamping device 402
Can be displaced under the action of third drives 403: micro-fluidic chip 1 can be clamped close to the position for being incubated for platform 302 by being moved to,
It is then displaced along the extending direction of detection platform 401 and micro-fluidic chip 1 is discharged in detection platform 401.At this point, being located at inspection
The detection device 405 for surveying 401 top of platform can carry out sample detection to micro-fluidic chip 1.In the preferred embodiment of the present embodiment,
Being incubated for platform 302 can match with the position of detection platform 401, when be incubated for platform 302 and 401 phase of detection platform close to when,
The placement direction on incubation platform 302 of micro-fluidic chip 1 is consistent with the extending direction of detection platform 401, fills clamping
Set 402 in one direction shift reciprocately can, be also convenient for clamping device 402 clamp micro-fluidic chip 1；And detection device 405
Position can be matched with the unloading position of micro-fluidic chip 1, clamped when clamping device 402 unclamps, parked micro-fluidic chip 1
When in detection platform 401, detection device 405 is placed exactly in above the detection zone of micro-fluidic chip 1.
And automatic tester provided in this embodiment further includes having control device, it can under the automatic control of control device
To realize the automatic operating of the first driving, the second driving 305, third driving 403 and detection device 405.So set, automatic
The realization of detection, it is only necessary to which the multiple micro-fluidic chips 1 for having been injected into sample are put into each incubation component 3 one by one；Band sample is complete
At chemical reaction, under the drive of the second driving 305, pressurizing unit 303 rack cleaning vacuole 13 realize to micro-fluidic chip 1 from
Dynamic injection cleaning solution；Then under the drive of the first driving, make to unload detection components 4 one by one close to each incubation component 3；Then
Third driving 403 driving under, clamping device 402 make micro-fluidic chip 1 be detached from be incubated for component 3 and be located at detection platform 401
On；Subsequent micro-fluidic chip 1 is detected by the detection device 405 for being located at 401 top of detection platform.Compared with prior art,
Automatic tester whole process operation provided in this embodiment simplifies, detection is quick, can be to a batch of multiple micro-fluidic
The otherness that chip 1 carries out one by one quickly detection, reduces test, it is ensured that the accuracy of detection.
Specifically, each moving member for being incubated for component 3 of connection can be it is linear, moved back and forth along straight line path,
It can be annular shape, circumferentially rotated along itself.If linearly, moving member can be the conveyer belt covered on two rotating wheels,
First driving is two rotating wheels and the motor for driving rotating wheel.Platform 302 is incubated for install on the conveyor belt by mounting base 301
And the first end for being incubated for platform 302 is perpendicular to the direction of second end and the extending direction of conveyer belt, drive of the conveyer belt in motor
It is moved back and forth under dynamic along the length direction of itself, and unloads detection components 4 and conveyer belt side, clamping dress are located at by bracket 2
Set 402 and detection platform 401 can be incubated for platform 302 it is parallel.When incubation platform 302 is close to detection platform 401 and the two
First end with respect to timing, clamping device 402 can clamp micro-fluidic chip 1 by its along perpendicular to moving member direction haul out,
It is placed in detection platform 401.The reciprocating movement of conveyer belt can be realized by controlling the power output shaft positive and negative rotation of motor.
Certainly, moving member and the structure of the first driving are not limited to that moving member can be with annular in shape.Specifically,
In this embodiment, as shown in Figure 1, moving member is set as the turntable 6 being connected on bracket 2, extending direction, that is, turntable 6 of moving member
Circumferential direction, turntable 6 circumferentially rotate i.e. around center axis rotate.First driving includes the electricity for driving turntable 6 to rotate
The rotation axis of the power output shaft and turntable 6 of machine and connection motor, the first end of rotation axis are fixedly connected on the center of circle of turntable 6
Locate, the power output axis connection of second end and motor.Multiple incubation components 3 are fixedly connected on turntable 6 by mounting base 301,
Along the circumferentially distributed of turntable 6, multiple central axis for being incubated for component 3 with turntable 6 around turntable 6 are rotated, and unload detection group
Part 4 is located on the rotation path of turntable 6.As shown in Figure 1 or 2, when incubation component 3 is turned to turntable 6 close to unloading detection
The position of component 4, the first end of the first end and detection platform 401 that are incubated for platform 302, which comes close to or in contact with, to offset, and turntable 6 stops
Rotation, clamping device 402 are operated under the driving of third driving 403.
The upper surface of turntable 6 can be located at, be also possible to be connected to the lower end surface of turntable 6 by being incubated for component 3.
It is incubated for platform 302 to be connected in mounting base 301, in the preferred embodiment of the present embodiment, as shown in Figure 1, being incubated for flat
Platform 302 can be also consistent with the radial direction of turntable 6 along the radially extending of turntable 6, the extending direction of detection platform 401, is being incubated for
The first end of platform 302 and the first end of detection platform 401 are close or when offseting, and clamping device 402 is along the radial reciprocal of turntable 6
Displacement.In this way, can simplify the structure of third driving 403, micro-fluidic chip 1 is placed by the personnel in operation that are also convenient for is incubated
It educates on platform 302.Certainly, it is incubated for platform 302 and detection platform 401 is also possible to be arranged along perpendicular to the radial direction of turntable 6,
Details are not described herein again.
As shown in Figure 2 and Figure 5, the detection platform 401 for unloading detection components 4 is fixed on bracket 2,403 packet of third driving
Include motor, the lead screw with the power output axis connection of motor, the first end of the axial direction of lead screw and detection platform 401 to second end
Direction it is consistent, it is preferable that it is radially consistent with turntable 6.Clamping device 402 be connected through a screw thread structure be set on lead screw,
The displacement along lead screw axial direction is generated with the rotation of lead screw.The shift reciprocately of clamping device 402 can be by adjusting the positive and negative of motor
Transfer realization.
As shown in Figure 5 and Figure 6, clamping device 402 includes sliding shoe 422 and the intermediate plate that is connected on sliding shoe 422
421, the threaded hole penetrated for lead screw is provided on sliding shoe 422, and intermediate plate 421 is at least provided with two, in the present embodiment, with two
For a intermediate plate 421, two intermediate plates 421 are oppositely arranged and keep spacing and formed between two intermediate plates 421 to supply micro-fluidic core
The grasping part 423 that piece 1 protrudes into.When the radial displacement under drive of the clamping device 402 in third driving 403 along turntable 6 is to leaning on
When the nearly first end for being incubated for platform 302, two intermediate plates 421 correspond respectively to the opposite two side walls of micro-fluidic chip 1, work as folder
It holds device 402 to continue towards close to the direction displacement for being incubated for platform 302, the first end of micro-fluidic chip 1 protrudes into grasping part
423, two intermediate plates 421 are stuck on above-mentioned two opposite side wall.
When clamping device 402 close to be incubated for platform 302 when, clamping device 402 can be located at be incubated for platform 302 top,
Two intermediate plates 421 be located at the lower section of sliding shoe 422 so that be incubated for platform 302 on micro-fluidic chip 1 enter grasping part 423,
Clamping device 402 is also possible to be located at the lower section for being incubated for platform 302.
As shown in Figure 2, Figure 5 and Figure 6, in the present embodiment, it is located at same water due to being incubated for platform 302 and detection platform 401
In plane, clamping device 402 and third driving 403 can be mounted on to the lower section of detection platform 401.The electricity of third driving 403
Machine is fixedly connected on the lower end surface of detection platform 401.When clamping device 402 is close to incubation platform 302, due to two intermediate plates
421 will extend to be incubated for above platform 302 and clamp micro-fluidic chip 1.Then sliding shoe 422 for close to being incubated for platform 302
One end is provided with the groove protruded into for the end for being incubated for platform, and groove is located at the underface of intermediate plate 421, so that two intermediate plates 421
First end along lead screw it is axially projecting in sliding shoe 422, form step structure 425 between first end and sliding shoe.Two
The first end of intermediate plate 421 is referred to close to the one end for being incubated for platform 302.Be additionally provided on sliding shoe 422 for detection platform 401 and
The through slot 424 that micro-fluidic chip 1 protrudes into, and through slot 424 is connected with grasping part 423 and is located under grasping part 423
Side.So set, the first end for being incubated for platform 302 can protrude into step when clamping device 402 is moved to close to platform 302 is incubated for
In structure 425, the first end of micro-fluidic chip 1 enters grasping part 423 simultaneously.
The distance between the distance between two intermediate plates 421 two side walls match, and can be the size interference of the two
Fillet is set with the edge merged in the first end of two intermediate plates 421, is also possible to the former and is slightly less than the latter and is set on side wall
It is equipped with the sliding slot entered for intermediate plate 421.When making the first end of micro-fluidic chip 1 enter grasping part 423, two intermediate plates 421 are pressed from both sides
Tightly in the two side walls of first end.Then, motor rotate backward, with clamping device 402 far from being incubated for platform 302 towards close
The direction of detection platform 401 is mobile, and detection platform 401 enters in through slot 424, micro-fluidic chip 1 is located in detection platform 401
Side.When be moved to position that micro-fluidic chip 1 can be placed in detection platform 401 or be moved to detection device 405 just under
Side is moved to formulation position, and clamping device 402 unloads micro-fluidic chip 1 in this position.
The unloading operation of clamping device 402 can intercept part 404 by being arranged in movement routine, intercept part 404 and be located at
In detection platform 401 and in detection platform 401 first end and second end between, intercept part 404 perpendicular to lead screw
Size in axial direction should be less than the distance between two intermediate plates 421.When the first end and interception 404 phase of part of micro-fluidic chip 1
It supports, motor drives clamping device 402 to continue the second end movement to detection platform 401, then micro-fluidic chip 1 is intercepted part 404
It intercepts and continuously moves to completely disengage micro-fluidic chip 1 in situ, two intermediate plates 421, so that micro-fluidic chip 1 is discharged in
In detection platform 401.
Intercepting part 404 can be a block for intercepting block, being raised in detection platform 401, be also possible to stop micro-
Fluidic chip 1 continues to be displaced and can enter grasping part 423, so that two intermediate plates 421 can completely disengage micro-fluidic chip 1
Any structure of side wall.Certainly, the realization of unloading operation is not limited to the setting in movement routine and intercepts the such knot of part 404
Structure can also be that setting can make intermediate plate 421 along the axial direction position perpendicular to lead screw between intermediate plate 421 and sliding shoe 422
The driving structure of shifting, no longer repeats one by one herein.
When micro-fluidic chip 1 is in detection platform 401, detection device 405 can be detected micro-fluidic chip 1.
Detection device 405 is set as the detection device 405 detected in the prior art to the sample in micro-fluidic chip 1.The detection
Device 405 already belongs to the general technology in the field, specific structure and principle, is not in the improvement and protection of this patent
Hold, details are not described herein again.Detection device 405 in the prior art need to be only mounted on to 401 top of detection platform and put down with detection
Platform 401 keeps the spacing for being sufficient to accommodate micro-fluidic chip 1.
As depicted in figs. 1 and 2, in the present embodiment, automatic tester further includes having to detect for releasing micro-fluidic chip 1
The catch bar 5 of platform 401, the 4th driving for driving catch bar 5 and the discard bin 7 for accepting the micro-fluidic chip 1 fallen.It pushes away
The axial direction of lever 5 and the axial direction of lead screw are perpendicular and be in same level height with micro-fluidic chip 1.Catch bar 5 and 4 wheel driven
It is dynamic to be connect with bracket 2, positioned at the side of detection platform 401, and discard bin 7 is located at the other side of detection platform 401 and positioned at pushing away
On dynamic path, so that micro-fluidic chip 1 can directly be fallen in discard bin 7.4th driving can be set to cylinder, catch bar
5 rod pieces that can be set to the piston rod of cylinder or connect with piston rod.When micro-fluidic chip 1 is placed in detection platform 401,
It can be the edge that position, the edge in the edge close to detection platform 401 are the close discard bins 7 of detection platform 401.
Or the promotion displacement of catch bar 5 is greater than the distance at the edge of the close discard bin 7 of micro-fluidic chip 1 to detection platform 401.
Specifically, in each incubation component 3, the second driving 305 can be connected with pressurizing unit 303, extruding is driven to fill
It sets 303 displacements to come close to platform 302 is incubated for, e.g., cleaning vacuole 13 is located at the first end of micro-fluidic chip 1 namely rising for fluid channel
Beginning, the first end of micro-fluidic chip 1 are placed on the first end for being incubated for platform 302, and pressurizing unit 303, which is located at, is incubated for platform 302
First end top, with cleaning vacuole 13 relatively just.Pressurizing unit 303 can be pricker or be crushing block, the second driving
305 be the telescopic rod of end connection pressurizing unit 303, when telescopic rod stretches out, pushes pressurizing unit 303 and is moved to and cleans downwards
Vacuole 13, which offsets, can puncture cleaning vacuole 13, automatically flow into cleaning solution in fluid channel.Telescopic rod can be the piston of cylinder
The piston rod of bar or hydraulic cylinder.Alternatively, the second driving 305 can be connected with platform 302 is incubated for, drive incubation 302, platform
It moves close to pressurizing unit 303.
It is also possible that as shown in Figure 3 and Figure 4, in the present embodiment, the incubation platform 302 for being incubated for component 3 passes through shaft
304 are rotatably connected in mounting base 301, and the second driving 305 connect with incubation platform 302, incubation platform 302 is driven to surround
Shaft 304 rotates, and pressurizing unit 303 is located on the rotation path for being incubated for platform 302.Specifically, the axial direction and incubation of shaft 304
It is plane parallel where platform 302, perpendicular with the direction of first end to the second end of incubation platform 302.Shaft 304 connects
In the position for the separate first end for being incubated for platform 302, so that the first end for being incubated for platform 302 is incubated for flat around the rotation of shaft 304
Platform 302 may be at the heeling condition that first end is higher than second end.Second driving 305 includes motor, defeated with the power of motor
Out the driving gear of axis connection and be incubated for the driven tooth plate that connect of platform 302, driven tooth plate is meshed transmission with driving gear,
Driven tooth plate is connected on the side wall for being incubated for platform 302 and the axis of the center of rotation and shaft 304 of the gear teeth coincides.In this way,
When motor rotates, by gear drive, being incubated for platform 302 can be rotated around shaft 304.It is incubated for the rotation of platform 302
Angular range can be realized by adjusting the total angular range of the gear teeth on driven tooth plate.
Pressurizing unit 303, which can be set to crushing block, be fixedly connected in mounting base 301 and is located at, is incubated for platform 302
On the rotation path of first end.It is micro-fluidic when micro-fluidic chip 1 by horizontal position turns to first position with platform 302 is incubated for
Chip 1 and incubation platform 302 are in heeling condition of the first end higher than second end and cleaning vacuole 13 and crushing block offset simultaneously
Briquetting is squeezed to rack.So set, cleaning vacuole 13 can be racked automatically when micro-fluidic chip 1 is in heeling condition, clearly
Washing lotion is injected in inclined fluid channel, can accelerate flowing velocity of the cleaning solution in fluid channel, and cleaning process is accelerated, and saves whole
The body time；And first position is adjustable, it is only necessary to adjust pressurizing unit 303 position or crushing block with cleaning 13 phase of vacuole
The tilt angle for the end face supported can change pressurizing unit 303 and clean the inclination angle for being incubated for platform 302 when vacuole 13 offsets
Degree, allows micro-fluidic chip 1 to be in different heeling conditions；And micro-fluidic chip 1 is in different heeling conditions, on the one hand
Flowing velocity of the adjustable cleaning solution in fluid channel, on the other hand, since in the prior art, sample flows in fluid channel
Occur to chemically react in the process and needs just to flow to fluid channel from the interlude position of fluid channel after the completion of chemical reaction
Terminal position, then micro-fluidic chip 1 be in heeling condition, can also the chemical reaction according to needed for different samples duration not
With and the specific flowing velocity for adjusting sample in fluid channel, shortening sample is flow to from fluid channel starting point to varying degrees
The time of fluid channel end.
Micro-fluidic chip 1 can be fixed on by rope or rubber band or magnetic suck structure to be incubated on platform 302, or
It is incubated for the second end setting block of platform 302, micro-fluidic chip 1 is stopped to slide when being in heeling condition.For for micro-fluidic core
The electric heater unit that piece 1 heats can be the lower section for being laid on and being incubated for platform 302, and electric heater unit can be electric heating wire can also
To be electric heating film.Each electric heater unit for being incubated for component 3 can be electrically connected with temperature sensor and temperature control equipment,
Such as temperature controller or temperature relay, in order to carry out heated at constant temperature to micro-fluidic chip 1, and heating temperature can be carried out specific
Setting.
In the preferred embodiment of the present embodiment, it is arranged on a rotating block as shown in figure 3, being incubated for platform 302 and can be, turns
Cavity open at one end is provided on motion block, the bottom wall of cavity forms and is incubated for platform 302, the open end of cavity is to be incubated for platform
302 first end, and be incubated for platform 302 first end extend for cavity opening, so that micro-fluidic chip 1 cleaning vacuole
13 are located at except cavity, and micro-fluidic chip 1, which can be inserted in cavity, to be fixed.Meanwhile so set, the inner wall of cavity can be with
It is equipped with electric heating film, carry out comprehensive to micro-fluidic chip 1 while being heated.And cavity can be through injection molding and rotating block
It is integrally formed, is also possible to rotating block and is formed by the cover plate combination for having reeded pedestal and being connected with pedestal, the length of groove
Open at one end, the cover board in degree direction cover in most groove and form above-mentioned cavity.
In order to realize it is each be incubated for component 3 incubation platform 302 synchronous rotation, it is each second driving 305 motor with
The global switch of the motor for all second drivings 305 that are same as being turned on or off is electrically connected between power supply, and it is each in order to realize
It is incubated for the independent rotation of platform 302, is electrically connected with independent switch between the motor and global switch of each second driving 305.
Alternatively, in the present embodiment, automatic tester further includes having for controlling the whole conducting of each second driving 305 or solely
The motor of the control device of vertical conducting, control device and the first driving, the motor of each second driving 305, third drive 403
Cylinder equal power device is electrically connected, and the connection that can be communicated with detection device 405.So it may be implemented to entire detection process
Automation control.Detection device 405 can be set to the controller for having communication connection function in the prior art, such as single-chip microcontroller
Or PLC, it is preferable that PLC can be used, so can directly be bought on the market.It is Siemens s7- that PLC, which can select model,
The PLC of 200smart.On this basis, each electric heater unit for being incubated for component 3 and temperature sensor can also be with single-chip microcontrollers
Or PLC is electrically connected, and is realized by circuit control that single-chip microcontroller or PLC are turned on or off to each electric heater unit
To the automatic computer heating control of micro-fluidic chip 1.
In the present embodiment, to prevent external dust or impurity into the sample that reaction takes place, bracket 2 is externally provided with one
The cabinet of bracket 2 can be covered, the inlet being connected to in case is provided on the side wall of cabinet, inlet, which is located at, is incubated for component 3
Rotation path on.Be incubated for being arranged radially along turntable 6 of platform 302, for micro-fluidic chip 1 be inserted into first end relative to incubation
The second end of platform 302, the position in separate 6 center of circle of turntable.When each incubation component 3 successively turns to the position of inlet
It sets, inlet and is incubated for the first end of platform 302 relatively just, operator or other automatic equipments will have been injected into the miniflow of sample
Control chip 1 protrudes into inlet, puts on the incubation platform 302 of incubation component 3.Then the incubation component 3 leaves this position, is turning
Sample chemically reacts during dynamic, band after the reaction was completed, the incubation component 3 turn to unload the positions of detection components 4 into
The unloading and detection of row micro-fluidic chip 1.Preferably, inlet can be adjacent with its positioned at the side for unloading detection components 4.
The present embodiment additionally provides a kind of automatic checkout system, includes micro-fluidic chip 1 and for detecting micro-fluidic core
The automatic tester of sample in piece 1, the automatic tester are automatic tester as described in the examples as above, the then automatic detection
System, which has that multiple micro-fluidic chips 1 operate simultaneously, multisample is tested and detected simultaneously and can be realized, quickly to be detected
Advantage.The derivation process of the beneficial effect and the derivation process of beneficial effect brought by above-mentioned automatic tester are substantially similar,
Repeats no more herein.
As shown in Figure 7 and Figure 8, in the preferred embodiment of this implementation, micro-fluidic chip 1 in automatic checkout system, miniflow
Road is in enclosed construction and includes the sample-adding storehouse 101 being sequentially communicated, mixing bunker 102, delay path 103 and waste liquid storehouse 104.It is micro-
Fluidic chip 1 further include the cleaning vacuole 13 for having built-in cleaning solution, for accommodate cleaning vacuole 13 cleaning solution storehouse 105 be connected to
The injection channel 106 in cleaning solution storehouse 105 and sample-adding storehouse 101.Cleaning vacuole 13 can be bag film and be wrapped in cleaning solution and be formed,
Bag film, built-in cleaning solution can such as be made of plastic film and aluminium foil.It is incubated on platform 302 when micro-fluidic chip 1 is placed on
And rotate with it to pressurizing unit 303 and be in contact, pressurizing unit 303 can rack cleaning vacuole 13, make to clean vacuole 13
In cleaning solution flow into the sample-adding storehouse 101 for injecting fluid channel in cleaning solution storehouse 105 and by injection channel 106, then from adding
The operation that sample storehouse 101 flows to waste liquid storehouse 104, completes cleaning fluid channel.
The sample-adding storehouse 101 of fluid channel is located at the starting point of fluid channel, is provided in adding mouth 107 and sample-adding storehouse 101 and sets
It is equipped in the solid-state like reagent ball for being chemically reacted with sample.So set, reagent ball sealing is stored in fluid channel, when
When being tested, operator only needs to inject sample into sample-adding storehouse 101 by adding mouth 107, and sample is adding
Reagent ball is dissolved in sample storehouse 101 and is thoroughly mixed with the reagent in reagent ball, is also conducive to mixed adequacy promotion chemical reaction
It is comprehensive.
And mixing bunker 102 be connected to sample-adding storehouse 101, for make sample in flowing and be dissolved in its reagent carry out it is abundant,
Uniformly mixing.Be provided with delay path 103 between mixing bunker 102 and waste liquid storehouse 104, delay path 103 be set as it is multiple side by side
Setting and the sequentially connected capillary channel structure of head and the tail, to be in curved S-shaped channel.The stream that sample will be slow from mixing bunker 102
Enter delay path 103 and in channel Tortuous flow, need to spend longer time come by delay path 103, ensure that
Time needed for sample sufficiently completes chemical reaction, waste liquid storehouse 104 can be just flowed into after so that sample is completed chemical reaction.So set
It sets, micro-fluidic chip 1 and the automatic tester of the structure are used cooperatively, and can preferably play the incubation platform for being incubated for component 3
302 rotating effect, can the preferably flowing velocity of Control Assay or cleaning solution in fluid channel.
As shown in fig. 7, micro-fluidic chip 1 can be the upper cover 11 by having reeded substrate 12 and being connected with substrate 12
Composition, upper cover 11 cover the fluid channel that groove forms enclosed construction.Herein it should be noted that the label in Fig. 7 identified
It is the position of each component.
Adding mouth 107 is respectively positioned in upper cover 11 with venthole 108 and is connected with fluid channel.Cleaning solution storehouse 105 is located at upper
On lid 11, and cleaning solution storehouse 105 is provided with opening, and the starting point for the groove for forming fluid channel on substrate 12 is provided with injection channel
106, injection channel 106 is connected with sample-adding storehouse 101.After substrate 12 is connect with upper cover 11, opening is opposite with injection channel 106
It just and is connected to, after cleaning vacuole 13 is squeezed and broken, cleaning solution flows into injection channel 106 from opening, subsequently into sample-adding storehouse
101, finally flow into waste liquid storehouse 104.
And it is can wrap in order to realize the absorption of the conjugate to chemical reaction production, in reagent ball containing exempting from sample
The antibody/antigen for being coated with fluorescent marker and magnetic bead of epidemic disease reaction, so that containing fluorescent marker and magnetic bead in conjugate.In
It is provided with adsorption zone between waste liquid storehouse 104 and delay path 103, is equipped on the bottom wall of adsorption zone and generates suction-operated with magnetic bead
Magnet, when sample flows through adsorbate, conjugate is attracted to Di Bishang, remaining sample flows into waste.It has been coated with fluorescence mark
The antibody/antigen of note object and magnetic bead belongs to existing product in the prior art.
It should be noted that top, lower section, upper surface and lower end surface etc. are related to the word in orientation in above-mentioned each embodiment
In language, the upper and lower upper and lower orientation referred in attached drawing.
It is understood that same or similar part can mutually refer in the various embodiments described above, in some embodiments
Unspecified content may refer to the same or similar content in other embodiments.Multiple schemes provided by the invention include
The basic scheme of itself does not condition each other independently of each other, but it can also be combined with each other in the absence of conflict, reach
It is realized jointly to multiple effects.
The foregoing description of the disclosed embodiments enables those skilled in the art to implement or use the present invention.
Various modifications to these embodiments will be readily apparent to those skilled in the art, as defined herein
General Principle can be realized in other embodiments without departing from the spirit or scope of the present invention.Therefore, of the invention
It is not intended to be limited to the embodiments shown herein, and is to fit to and the principles and novel features disclosed herein phase one
The widest scope of cause.
Claims (10)
1. a kind of automatic tester, which is characterized in that include bracket (2), the incubation component for carrying micro-fluidic chip (1)
(3), for being detached from the micro-fluidic chip (1) described component (3) be incubated for and the micro-fluidic chip (1) is detected
Unload detection components (4) and control device；
Moving member is provided on the bracket (2) and for driving the moving member reciprocal along straight line path or closed circle path
First driving of displacement；
The incubation component be arranged it is multiple and along the extending direction of the moving member be distributed, the incubation component include with it is described
Mounting base (301) that moving member is fixedly connected is connected on the mounting base (301) for carrying the micro-fluidic chip (1)
Incubation platform (302), connect with the mounting base (301) pressurizing unit (303), for driving the incubation platform and institute
It states second driving (305) of pressurizing unit (303) relative displacement, be located on the incubation platform (302) for the incubation
The electric heater unit of platform (302) heating, when second driving (305) drives the incubation platform and the pressurizing unit
(303) close, the described pressurizing unit (303) can rack the cleaning vacuole (13) of the micro-fluidic chip (1)；
Unloading detection components (4) is located in the side of the moving member and the moving range in the moving member, described
Unloading detection components (4) includes the detection platform (401) being in same level with incubation platform (302), connect
Clamping device (402) on the detection platform (401), for driving the clamping device (402) along close to or far from institute
It states the third driving (403) for the direction displacement for being incubated for platform (302) and is connected on the detection platform (401) and is used to detect
The detection device (405) of the interior sample of the micro-fluidic chip (1), when the incubation platform is close to the detection platform (401),
Clamping device (402) clamping micro-fluidic chip (1) is displaced along the extending direction of the detection platform (401) and will
Enough micro-fluidic chips (1) are discharged on the detection platform (401)；
The control device with it is described first driving, it is described second driving (305), the third driving (403) be electrically connected and with
Detection device (405) communication connection.
2. automatic tester as described in claim 1, which is characterized in that the incubation platform (302) can by shaft (304)
It is rotationally connected on the mounting base (301), and where the axial direction of the shaft (304) and the incubation platform (302)
Plane parallel, shaft (304) connection perpendicular with the direction of first end to the second end for being incubated for platform (302)
In the position far from the first end for being incubated for platform (302)；Second driving (305) and the incubation platform
(302) it connects and rotates the incubation platform (302) around the shaft (304)；The pressurizing unit (303) is located at described
It is incubated on the rotation path of platform (302), when the incubation platform (302) turns to first position, the incubation platform
(302) first end is higher than the second end and the pressurizing unit (303) offsets and can squeeze with the cleaning vacuole (13)
Break the cleaning vacuole (13).
3. automatic tester as described in claim 1, which is characterized in that further include having for by the micro-fluidic chip (1)
The 4th driving released the catch bar (5) of the detection platform (401), the catch bar (5) is driven to move along its axial reciprocating
And the discard bin (7) for holding the micro-fluidic chip (1), the 4th driving and the discard bin (7) are connected to described
On bracket (2) and the discard bin (7) is located on the pushing direction of the catch bar (5).
4. automatic tester as described in claim 1, which is characterized in that the moving member is set as being connected to the bracket
(2) turntable (6) on, first driving includes the motor that the driving turntable (6) surrounds center axis thereof rotation, described
The power output axis connection of turntable (6) and the motor, unloading detection components (4) are located at turn for being incubated for component (3)
On dynamic path.
5. automatic tester as described in claim 1, which is characterized in that third driving (403) includes and the branch
The lead screw of the power output axis connection of motor and the motor that frame (2) is fixedly connected, the clamping device (402) pass through spiral shell
Line connection structure is set on the lead screw, and the axial direction of the lead screw is consistent with the extending direction of the detection platform (401).
6. automatic tester as claimed in claim 5, which is characterized in that the clamping device (402) is provided with sliding shoe
(422) and it is connected to the intermediate plates (421) of the sliding shoe (422), is provided on the sliding shoe (422) and is protruded into for the lead screw
Threaded hole, the intermediate plate (421) is provided at least two, between being at least oppositely arranged and keep there are two the intermediate plate (421)
Away from, to form the grasping part (423) being caught in for the micro-fluidic chip (1), two intermediate plates (421) are for being stuck in
In the opposite two side walls of the micro-fluidic chip (1)；It is provided on the detection platform (401) for stopping the miniflow
The interception part (404) of chip (1) is controlled, interception part (404) is located in the movement routine of the grasping part (423), and
Perpendicular in the lead screw axially direction it is described intercept part (404) less than two intermediate plates (421) of size between away from
From.
7. automatic tester as claimed in claim 6, which is characterized in that it is flat that the third driving (403) is located at the detection
The lower section of platform (401) is provided with the through slot (424) protruded into for the detection platform (401), and institute on the sliding shoe (422)
State the lower section that through slot (424) is connected and is located at the grasping part (423) with the grasping part (423)；Two folders
The first end of piece (421) along the lead screw it is axially projecting in the sliding shoe (422), in the first end and the sliding
The step structure (425) protruded into for the end for being incubated for platform (302) is formed between block (422).
8. automatic tester as described in claim 1, which is characterized in that it further include having the cabinet for covering the bracket (2), institute
The inlet for being provided on cabinet and entering for the micro-fluidic chip (1) is stated, the inlet is arranged in the incubation component (3)
Rotation path on.
9. a kind of automatic checkout system includes micro-fluidic chip (1) and automatic tester, which is characterized in that the automatic inspection
Instrument is surveyed to be set as such as the described in any item automatic testers of claim 1-8.
10. automatic checkout system as claimed in claim 9, which is characterized in that the micro-fluidic chip (1) is provided with for sample
Flowing the fluid channel in enclosed construction, the fluid channel include the sample-adding storehouse (101) being sequentially communicated, mixing bunker (102),
Delay path (103), adsorption zone and waste liquid storehouse (104), sample-adding storehouse (101) are provided with the adding mouth being added for the sample
(107) and sample-adding storehouse (101) is provided with the solid state reagents ball being immunoreacted with the sample, wraps in the reagent ball
Contain the antibody/antigen for being coated with fluorescent marker and magnetic bead being immunoreacted with the sample, the bottom of the adsorption zone
The magnet for adsorbing the magnetic bead is equipped on wall；The delay path (103) is in multiple arranged side by side and successively connects from beginning to end
Logical capillary channel structure, the waste liquid storehouse (104) are provided with the venthole (108) communicated with ambient atmosphere；It further include having clearly
Washing lotion is steeped (13), the cleaning solution storehouse (105) for accommodating cleaning vacuole (13) and the connection cleaning solution storehouse (105) and is added with described
The injection channel (106) in sample storehouse (101).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910892663.1A CN110501514B (en) | 2019-09-20 | 2019-09-20 | Automatic detector and automatic detection system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910892663.1A CN110501514B (en) | 2019-09-20 | 2019-09-20 | Automatic detector and automatic detection system |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN110501514A true CN110501514A (en) | 2019-11-26 |
| CN110501514B CN110501514B (en) | 2023-12-22 |
Family
ID=68592231
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910892663.1A Active CN110501514B (en) | 2019-09-20 | 2019-09-20 | Automatic detector and automatic detection system |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110501514B (en) |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111039127A (en) * | 2019-12-24 | 2020-04-21 | 曾明霞 | Positioning and mounting equipment for guide rail inside elevator shaft |
| CN112973814A (en) * | 2021-03-03 | 2021-06-18 | 北京理工大学 | Interlayer automatic alignment bonding device and method for multilayer microfluidic chip |
| CN113552365A (en) * | 2021-06-16 | 2021-10-26 | 苏州捷美电子有限公司 | Western Blot full-automatic workstation |
| CN114509575A (en) * | 2022-04-19 | 2022-05-17 | 天津德祥生物技术有限公司 | Microfluidic detection device |
| CN114917972A (en) * | 2022-05-27 | 2022-08-19 | 圣湘生物科技股份有限公司 | Molecule detection device, molecule processing method, and detection method |
| CN116165094A (en) * | 2023-04-25 | 2023-05-26 | 江苏维科特仪器仪表有限公司 | Moisture detector with self-cleaning structure |
| CN116559389A (en) * | 2023-07-06 | 2023-08-08 | 北京中医药大学 | Device and method for detecting Chinese medicine property and taste |
| CN117505098A (en) * | 2023-12-29 | 2024-02-06 | 天津芥子生物技术有限公司 | Control method and system for multidimensional track position adjustment and track system |
| CN117505098B (en) * | 2023-12-29 | 2024-04-30 | 天津芥子生物技术有限公司 | Control method and system for multidimensional track position adjustment and track system |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1523354A (en) * | 2003-02-17 | 2004-08-25 | 中国科学院力学研究所 | Method and apparatus for bio-molecular chip minute quantity sample application and reaction |
| CN102046806A (en) * | 2008-05-09 | 2011-05-04 | 阿科尼生物系统公司 | Microarray system |
| CN106434282A (en) * | 2016-10-27 | 2017-02-22 | 杭州安杰思生物科技有限公司 | Micro-fluidic vacuole extrusion mechanism |
| CN107219360A (en) * | 2017-07-03 | 2017-09-29 | 苏州岚轩生物科技有限公司 | Single channel chemiluminescence micro-fluidic chip and its detection method |
| CN107384776A (en) * | 2017-08-04 | 2017-11-24 | 深圳市合川医疗科技有限公司 | Micro-fluidic chip |
| CN107843583A (en) * | 2017-10-16 | 2018-03-27 | 南京岚煜生物科技有限公司 | A kind of micro-fluidic chemiluminescent analyzer and application method |
| CN108535239A (en) * | 2018-03-28 | 2018-09-14 | 上海艾瑞德生物科技有限公司 | Micro-fluidic chip based on microlayer model and detecting system |
| CN109999931A (en) * | 2019-04-18 | 2019-07-12 | 天津诺迈科技有限公司 | Chemiluminescence detection micro-fluidic chip and application method, reagent cleaning method |
| CN211043416U (en) * | 2019-09-20 | 2020-07-17 | 四川微康朴澜医疗科技有限责任公司 | Automatic detector and automatic detection system |
-
2019
- 2019-09-20 CN CN201910892663.1A patent/CN110501514B/en active Active
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1523354A (en) * | 2003-02-17 | 2004-08-25 | 中国科学院力学研究所 | Method and apparatus for bio-molecular chip minute quantity sample application and reaction |
| CN102046806A (en) * | 2008-05-09 | 2011-05-04 | 阿科尼生物系统公司 | Microarray system |
| CN106434282A (en) * | 2016-10-27 | 2017-02-22 | 杭州安杰思生物科技有限公司 | Micro-fluidic vacuole extrusion mechanism |
| CN107219360A (en) * | 2017-07-03 | 2017-09-29 | 苏州岚轩生物科技有限公司 | Single channel chemiluminescence micro-fluidic chip and its detection method |
| CN107384776A (en) * | 2017-08-04 | 2017-11-24 | 深圳市合川医疗科技有限公司 | Micro-fluidic chip |
| CN107843583A (en) * | 2017-10-16 | 2018-03-27 | 南京岚煜生物科技有限公司 | A kind of micro-fluidic chemiluminescent analyzer and application method |
| CN108535239A (en) * | 2018-03-28 | 2018-09-14 | 上海艾瑞德生物科技有限公司 | Micro-fluidic chip based on microlayer model and detecting system |
| CN109999931A (en) * | 2019-04-18 | 2019-07-12 | 天津诺迈科技有限公司 | Chemiluminescence detection micro-fluidic chip and application method, reagent cleaning method |
| CN211043416U (en) * | 2019-09-20 | 2020-07-17 | 四川微康朴澜医疗科技有限责任公司 | Automatic detector and automatic detection system |
Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111039127A (en) * | 2019-12-24 | 2020-04-21 | 曾明霞 | Positioning and mounting equipment for guide rail inside elevator shaft |
| CN111039127B (en) * | 2019-12-24 | 2021-03-05 | 西威电梯江苏有限公司 | Positioning and mounting equipment for guide rail inside elevator shaft |
| CN112973814A (en) * | 2021-03-03 | 2021-06-18 | 北京理工大学 | Interlayer automatic alignment bonding device and method for multilayer microfluidic chip |
| CN112973814B (en) * | 2021-03-03 | 2022-03-18 | 北京理工大学 | Interlayer automatic alignment bonding device and method for multilayer microfluidic chip |
| CN113552365A (en) * | 2021-06-16 | 2021-10-26 | 苏州捷美电子有限公司 | Western Blot full-automatic workstation |
| CN114509575B (en) * | 2022-04-19 | 2022-06-14 | 天津德祥生物技术有限公司 | Microfluidic detection device |
| CN114509575A (en) * | 2022-04-19 | 2022-05-17 | 天津德祥生物技术有限公司 | Microfluidic detection device |
| CN114917972A (en) * | 2022-05-27 | 2022-08-19 | 圣湘生物科技股份有限公司 | Molecule detection device, molecule processing method, and detection method |
| CN114917972B (en) * | 2022-05-27 | 2024-04-09 | 圣湘生物科技股份有限公司 | Molecular detection device, molecular processing and detection method |
| CN116165094A (en) * | 2023-04-25 | 2023-05-26 | 江苏维科特仪器仪表有限公司 | Moisture detector with self-cleaning structure |
| CN116559389A (en) * | 2023-07-06 | 2023-08-08 | 北京中医药大学 | Device and method for detecting Chinese medicine property and taste |
| CN116559389B (en) * | 2023-07-06 | 2023-10-20 | 北京中医药大学 | Device and method for detecting Chinese medicine property and taste |
| CN117505098A (en) * | 2023-12-29 | 2024-02-06 | 天津芥子生物技术有限公司 | Control method and system for multidimensional track position adjustment and track system |
| CN117505098B (en) * | 2023-12-29 | 2024-04-30 | 天津芥子生物技术有限公司 | Control method and system for multidimensional track position adjustment and track system |
Also Published As
| Publication number | Publication date |
|---|---|
| CN110501514B (en) | 2023-12-22 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN110501514A (en) | Automatic tester and automatic checkout system | |
| CN105572407B (en) | Full-automatic fluorescent quantitation immunity analysis instrument and detection method | |
| US4837159A (en) | Method and apparatus for effecting immunological analysis | |
| CN207181272U (en) | Magnetic bead cleans separator and immunity analysis instrument automatically | |
| EP3671218A1 (en) | Blood analyzer and control method therefor | |
| CN107045069A (en) | A kind of specific protein analyzer reaction cup handling system and its application method | |
| CN109406799B (en) | Sample analysis apparatus, reagent adding device, and control method thereof | |
| CN207866716U (en) | Chemiluminescence detector | |
| CN105388314A (en) | Fully automated sample adding instrument | |
| CN107942085A (en) | Automatic analysing apparatus and its method of sample analysis | |
| CN104722526B (en) | A kind of magnetic separating device | |
| CN205844341U (en) | A kind of full-automatic illumination immunity analysis instrument | |
| JPH08211068A (en) | Method for operating analysis unit used for different immunological test | |
| CN109975562A (en) | Chemiluminescence detector and its detection method | |
| CN211043416U (en) | Automatic detector and automatic detection system | |
| CN105416628A (en) | Blood type detection card filling machine | |
| CN108732163A (en) | Full-automatic chemiluminescence immunoassay analysis meter | |
| CN111562400A (en) | Cleaning device and chemiluminescence immunoassay appearance | |
| CN114019178A (en) | Full-automatic immune biochemical integrated analyzer and use method thereof | |
| CN206892198U (en) | A kind of full encapsulation condition bottom sheet shape capacitance testing device | |
| CN212646715U (en) | Full-automatic analyzer | |
| CN110501491B (en) | Multi-channel incubation device capable of supporting chip inclination and sample preparation equipment | |
| CN205193087U (en) | Automatic spotting instrument | |
| CN206684176U (en) | A kind of specific protein analyzer reaction cup handling system | |
| CN206740787U (en) | Multichannel magnetic microparticle chemiluminescence immune assay instrument test solution suction means |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |