CN113552365A - Western Blot full-automatic workstation - Google Patents
Western Blot full-automatic workstation Download PDFInfo
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- CN113552365A CN113552365A CN202110668442.3A CN202110668442A CN113552365A CN 113552365 A CN113552365 A CN 113552365A CN 202110668442 A CN202110668442 A CN 202110668442A CN 113552365 A CN113552365 A CN 113552365A
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- 238000001262 western blot Methods 0.000 title claims abstract description 22
- 238000006243 chemical reaction Methods 0.000 claims abstract description 42
- 239000007788 liquid Substances 0.000 claims abstract description 26
- 238000011534 incubation Methods 0.000 claims abstract description 16
- 239000002184 metal Substances 0.000 claims abstract description 7
- 229910052751 metal Inorganic materials 0.000 claims abstract description 7
- 238000005406 washing Methods 0.000 claims abstract description 7
- 238000005057 refrigeration Methods 0.000 claims abstract description 5
- 238000005070 sampling Methods 0.000 claims description 26
- 238000000034 method Methods 0.000 claims description 14
- 230000007246 mechanism Effects 0.000 claims description 11
- 230000005540 biological transmission Effects 0.000 claims description 5
- 230000006835 compression Effects 0.000 claims description 3
- 238000007906 compression Methods 0.000 claims description 3
- 238000009434 installation Methods 0.000 claims 2
- 238000001746 injection moulding Methods 0.000 claims 1
- 238000005213 imbibition Methods 0.000 abstract description 4
- 238000012546 transfer Methods 0.000 abstract description 2
- 230000008569 process Effects 0.000 description 9
- 238000010586 diagram Methods 0.000 description 8
- 108090000623 proteins and genes Proteins 0.000 description 7
- 239000012528 membrane Substances 0.000 description 6
- 235000018102 proteins Nutrition 0.000 description 6
- 102000004169 proteins and genes Human genes 0.000 description 6
- 238000004140 cleaning Methods 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 238000001962 electrophoresis Methods 0.000 description 3
- 238000002347 injection Methods 0.000 description 3
- 239000007924 injection Substances 0.000 description 3
- 238000011084 recovery Methods 0.000 description 3
- 239000007790 solid phase Substances 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000005611 electricity Effects 0.000 description 2
- 238000000465 moulding Methods 0.000 description 2
- 229920003023 plastic Polymers 0.000 description 2
- 239000004033 plastic Substances 0.000 description 2
- 238000002264 polyacrylamide gel electrophoresis Methods 0.000 description 2
- 229920001184 polypeptide Polymers 0.000 description 2
- 102000004196 processed proteins & peptides Human genes 0.000 description 2
- 108090000765 processed proteins & peptides Proteins 0.000 description 2
- 238000007789 sealing Methods 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 238000001179 sorption measurement Methods 0.000 description 2
- 238000010186 staining Methods 0.000 description 2
- 230000003068 static effect Effects 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 238000000376 autoradiography Methods 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000012864 cross contamination Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 238000001502 gel electrophoresis Methods 0.000 description 1
- 238000003018 immunoassay Methods 0.000 description 1
- 230000002998 immunogenetic effect Effects 0.000 description 1
- 230000036046 immunoreaction Effects 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000002991 molded plastic Substances 0.000 description 1
- 229920001220 nitrocellulos Polymers 0.000 description 1
- 230000000750 progressive effect Effects 0.000 description 1
- 235000004252 protein component Nutrition 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000010023 transfer printing Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Immunology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
The invention relates to a Western Blot full-automatic workstation, which comprises a rack, a refrigeration module, an antibody storage module, an incubation module, a manipulator, an electromagnet, an elastic sample adding needle, a sample injector, a liquid transfer workbench, a needle washing groove and a main control board, wherein the manipulator is used for automatically taking and placing a reaction box; the working platform of the liquid transferring working platform is wedge-shaped, and the upper surface of the working platform is provided with an inclined plane with a high front part and a low back part; the bottom of workstation inclined plane is equipped with the limiting plate, and the limiting plate intermediate position is equipped with location magnet for adsorb the magnetic metal piece on the reaction box body, make the imbibition more thorough, the location is more accurate.
Description
Technical Field
The invention relates to the field of Western Blot experiments, in particular to a Western Blot full-automatic workstation.
Background
Western Blot assay, is a commonly used assay in molecular biology, biochemistry and immunogenetics, and its basic principle is a method of staining a gel electrophoresis-treated cell or biological tissue sample with a specific antibody, and obtaining information on the expression of a specific protein in the analyzed cell or tissue by analyzing the location and depth of staining. The method can be divided into 3 stages of electrophoresis, transfer printing and enzyme immunoassay, wherein a protein sample separated by PAGE (polyacrylamide gel electrophoresis) is transferred onto a solid phase carrier (such as a nitrocellulose film), the solid phase carrier adsorbs the protein in a non-covalent bond form and can keep the type and the biological activity of the polypeptide separated by electrophoresis unchanged, the protein or the polypeptide on the solid phase carrier is taken as an antigen and has immunoreaction with a corresponding antibody, then the antibody reacts with a second antibody labeled by enzyme or isotope, and the protein component expressed by a specific target gene separated by electrophoresis is detected by substrate color development or autoradiography.
At present, in a Western Blot experiment, after membrane conversion is completed, full-automatic control cannot be achieved from sealing, primary antibody incubation, primary antibody recovery, primary antibody cleaning, secondary antibody incubation, secondary antibody recovery and secondary antibody cleaning, the whole process is tedious, repeated and boring, and failure of the whole process can be caused by each step of error, so that repeated experiments are carried out, and more, a rare sample needs to be carefully doubled. Meanwhile, the experimental process is long, the result repeatability is poor, and the cost of manpower, material resources and time is high to achieve a good experimental result. This application is improved to application of sample needle, accurate positioning platform and automation module for the imbibition is more thorough, and the location is more accurate.
Disclosure of Invention
In order to solve the technical problems, the invention provides a Western Blot full-automatic workstation which comprises a frame, a refrigeration module, an antibody storage module, an incubation module, a manipulator, an electromagnet, an elastic sample adding needle, a sample injector, a liquid transferring workbench, a needle washing groove and a main control board, wherein the manipulator is used for automatically taking and placing a reaction box; the working platform of the liquid transferring working platform is wedge-shaped, and the upper surface of the working platform is provided with an inclined plane with a high front part and a low back part; the bottom of the inclined plane of the workbench is provided with a limiting plate, and the middle position of the limiting plate is provided with a positioning magnet for adsorbing magnetic metal sheets on the box body of the reaction box.
Further, the locating pin includes preceding locating pin and back locating pin, locating pin upper portion is the toper, the little lower part in upper portion is big, move the liquid workstation below and have the mount table, workstation backup pad one end support table, the mount table is connected to the other end, the reaction box body is formed by moulding plastics, it is equipped with magnet to cover the four corners, reaction box body and lid pass through magnet and closely cooperate, cover and be equipped with the through-hole that supplies the application of sample needle to pass, cover and have the transparent part that supplies the observation usefulness, the reaction box bottom end is slight arc.
Furthermore, the elastic sample adding needle comprises a sample adding needle connecting sleeve, a sample adding needle fixing seat, a spring, a sample adding needle fastening sleeve and a sample adding needle, wherein the lower part of the sample adding needle connecting sleeve is in threaded connection with the sample adding needle fastening sleeve; the sampling needle connecting sleeve is fixed on the inner wall of the sampling needle fixing seat; the sample adding needle is partially arranged inside the sample adding needle connecting sleeve and the sample adding needle fastening sleeve, the spring sleeve is sleeved on the sample adding needle and is arranged in an inner cavity of the sample adding needle connecting sleeve, the inner cavity of the sample adding needle connecting sleeve is provided with a limiting step for controlling the compression stroke of the spring, and the periphery of the sample adding needle is provided with a step.
Furthermore, the upper end of the sample adding needle fixing seat is provided with a limiting piece which is matched with the photoelectric switch and used for controlling the stroke of the sample adding needle, so that the sample adding needle is guaranteed to reach an appointed station for imbibition and sample adding, and the sample adding needle fixing seat is connected with the transmission mechanism.
Further, a working method of the Western Blot full-automatic workstation is characterized in that a manipulator is used for taking and placing the reaction box, and the manipulator is controlled by a main control board
Compared with the prior art, the invention provides a Western Blot full-automatic workstation, which has the following effects: in the imbibing process of the elastic loading needle mechanism, the needle point of the loading needle can be directly contacted with the bottom of the loading box, and the needle point of the loading needle is in soft contact with the bottom of the loading box, so that the imbibing residual quantity is reduced, and the loading needle is prevented from being stressed, deformed or damaged; the workbench of the accurate positioning platform is wedge-shaped, the upper surface of the workbench is provided with an inclined plane with a high front part and a low back part, the reaction box component can slide to the position limiting part at the lowest end of the inclined plane of the workbench after being placed on the workbench, so that accurate positioning is ensured, in addition, liquid in the incubation box can flow to the lowest end, and the internal liquid can be completely sucked in the liquid suction process; the positioning magnet on the limiting plate of the workbench is adsorbed with the magnetic metal sheet on the box body of the reaction box, so that the accurate positioning can be ensured; the upper part of the positioning pin is conical, the upper part is small, and the lower part is large, so that the reaction box is ensured to fall into a space defined by the positioning pin more easily, and the positioning of the reaction box after falling is ensured to be more accurate; the bottom end of the reaction box is slightly arc-shaped, and the bottom of the reaction box and the workbench are provided with a tiny arc-shaped gap, so that friction force is reduced, the magnetic iron is adsorbed downwards, meanwhile, the adsorption force of friction static electricity or liquid adhesion between the bottom of the box body and the workbench is reduced, and the box is taken and placed by a manipulator more easily.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is a schematic front view of a Western Blot fully automatic workstation according to the present invention;
FIG. 2 is a schematic diagram of the back side of a Western Blot fully automatic workstation according to the present invention;
FIG. 3 is another schematic diagram of the front side of the Western Blot fully automatic workstation of the invention;
FIG. 4 is a schematic diagram of an elastic sample adding needle of a Western Blot full-automatic workstation according to the present invention;
FIG. 5 is a schematic diagram of an elastic sample adding needle of a Western Blot full-automatic workstation according to the present invention;
FIG. 6 is a schematic diagram of a precise positioning platform of a Western Blot fully-automatic workstation according to the present invention;
FIG. 7 is a schematic diagram of a precise positioning platform of a Western Blot fully-automatic workstation according to the present invention;
FIG. 8 is a schematic diagram of a precise positioning platform of a Western Blot fully-automatic workstation according to the present invention;
FIG. 9 is a schematic diagram of the whole process flow of a single reaction cassette of a Western Blot full-automatic workstation of the present invention.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention, but the present invention may be practiced in other ways than those specifically described and will be readily apparent to those of ordinary skill in the art without departing from the spirit of the present invention, and therefore the present invention is not limited to the specific embodiments disclosed below.
As shown in fig. 1, 2 and 3, the Western Blot full-automatic workstation comprises a frame 1, a refrigeration module 2, an antibody storage module 3, an incubation module 4, a manipulator 5, an electromagnet 6, an elastic sample injection needle 7, a sample injector 8, a liquid transfer workbench 9, a needle washing groove 10, a touch screen 11, a main control board 12 and a top cover 13, wherein the refrigeration module 2 refrigerates the antibody storage module 3 and the incubation module 4 to realize low-temperature sample storage; the manipulator is used for taking and putting the reaction box 14 in a full-automatic mode, the manipulator comprises an X-axis motion mechanism, a Y-axis motion mechanism and a Z-axis motion mechanism, for example, an air cylinder, a belt wheel, a sliding rail and the like, and the main control board controls all the components.
The elastic sampling needle comprises a sampling needle connecting sleeve 15, a sampling needle fixing seat 16, a top tightening screw 17, a spring 18, a sampling needle fastening sleeve 19, a sampling needle 20, a limiting piece 21 and a screw 22, the spring is sleeved on the sampling needle and is arranged in the inner cavity of the sampling needle connecting sleeve, and the inner cavity of the sampling needle connecting sleeve is provided with a limiting step for controlling the compression stroke of the spring; the outer side of the sampling needle is provided with a step which is used for limiting the spring and limiting at least one part of the sampling needle in the sampling needle connecting sleeve, and the step is positioned at a position below the spring on the outer surface of the sampling needle; the sampling needle connecting sleeve is connected with the sampling needle fastening sleeve through threads and is used for fixing the sampling needle; the sample adding needle connecting sleeve is fixed on the sample adding needle fixing seat by a set screw, and the limiting piece is fixed on the sample adding needle fixing seat by a screw 8; the sample adding needle mechanism is connected with the transmission mechanism through the sample adding needle fixing seat and moves downwards under the driving of the transmission mechanism, the transmission mechanism is connected and matched with the photoelectric switch through the limiting piece, the zero position and the stroke of the whole sample adding needle mechanism are controlled, and the sample adding needle is guaranteed to accurately reach an appointed station; in the imbibition process, the application of sample needle contacts with application of sample box bottom, and application of sample needle upwards compresses the spring under the effect of contact force, and the spring is compressed in the inner chamber of application of sample needle adapter sleeve, and application of sample needle elasticity surplus in the inner chamber of application of sample needle adapter sleeve has protected application of sample needle because of the atress warp or damage.
The incubation module bears the reaction box, the reaction box includes reaction box body 23, magnetic metal sheet 24, incubate box 25 and reaction lid 26, the reaction box body is formed by moulding plastics, a magnetic metal sheet has been inlayed when the mould is moulded plastics, the incubation box is put into the reaction box body, the reaction box body passes through magnet close fit with the reaction lid, make the reaction box part move the in-process reaction lid that moves the liquid workstation by the manipulator all the time with reaction box body in close contact with, can not skew or drop. The cover is provided with through holes for the sample adding needle to pass through, and four corners of the cover are provided with magnets. The liquid transferring workbench comprises a rear positioning pin 27, a workbench 28, a workbench supporting plate 29, a mounting table 30, a front positioning pin 31, a positioning magnet 32 and a screw 33; the workbench of the liquid transferring workbench is wedge-shaped, the upper surface of the workbench is provided with an inclined plane with a high front part and a low back part, so that liquid in the incubation box flows to the lowest end, the liquid in the incubation box can be completely absorbed in the liquid absorbing process, and the reaction box component can slide to the limiting position at the lowest end of the inclined plane of the workbench after being placed on the workbench; a positioning magnet is arranged at the middle position of the limiting position of the workbench through a screw and used for adsorbing a magnetic metal sheet on the box body of the reaction box; still install a set of preceding locating pin and a set of back locating pin on the workstation, two sets of locating pin upper portions are the toper, upper portion is little, the lower part is big, both guarantee that the reaction box falls into the space that the locating pin is injectd more easily, guarantee again that the reaction box falls the back and fix a position more accurately, reaction box bottom end face is the bellied arc that makes progress slightly, reaction box bottom and workstation have little arc space, thereby reduce friction power, when more being favorable to by magnet absorption, more do benefit to the frictional static electricity that reduces bottom and workstation or the adsorption affinity that working liquid adheres, change and be got by the manipulator and put, fix a position more accurately. The liquid transferring worktable takes the mounting platform as a base, and two worktable supporting plates support the worktable and cooperate with specific reaction box components to jointly form an accurate positioning platform.
The top cover 13 plays a whole dustproof role, the main control board 12 controls the incubation module 4 to swing, the mechanical arm 5, the electromagnet 6, the sample injection needle 7 and the sample injector 8 are combined to automatically realize automatic sealing, automatic and full combination of the protein membrane, incubation, automatic membrane washing, automatic liquid injection, automatic waste liquid recovery and full-automatic program realization of the whole process of combining the protein membrane with the antibody and cleaning the membrane.
The liquid transferring workbench 9 provides an accurate positioning platform for automatically taking and placing the reaction box, and improves the precision of automatically sucking and spitting liquid of a subsequent sample adding needle; the needle washing groove 10 is used for washing the inner wall and the outer wall of the sample adding needle, the whole pipeline of the instrument can be washed while the needle is washed, cross contamination among different reagents is avoided, and the success rate of an experiment is ensured; up to 12 experimental schemes can be preset by operating the touch screen 11, the sample adding volume, the incubation time, the cleaning times and the swing time of each step can be automatically set, tedious, repeated and boring manual tests are replaced by instruments, the numerical control operation flow can avoid manual operation errors, the background is reduced, the signal-to-noise ratio and the relative sensitivity are improved, and the repeatability of the same batch and different batches is ensured; the instrument provides simultaneous operation of up to 12 membranes/gels, with independent parameter settings for each incubation well, and finally comparison of results for 12 different experimental conditions.
In the description, each part is described in a progressive manner, each part is emphasized to be different from other parts, and the same and similar parts among the parts are referred to each other. The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention.
Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (5)
1. A Western Blot full-automatic workstation comprises a rack, a refrigeration module, an antibody storage module, an incubation module, a manipulator, an electromagnet, an elastic sample adding needle, a sample injector, a liquid transferring workbench, a needle washing groove and a main control board, wherein the manipulator is used for automatically taking and placing a reaction box; the working platform of the liquid transferring working platform is wedge-shaped, and the upper surface of the working platform is provided with an inclined plane with a high front part and a low back part; the bottom of the inclined plane of the workbench is provided with a limiting plate, and the middle position of the limiting plate is provided with a positioning magnet for adsorbing magnetic metal sheets on the box body of the reaction box.
2. The platform of claim 1, wherein the positioning pins comprise a front positioning pin and a rear positioning pin, the upper part of the positioning pin is conical, the upper part of the positioning pin is small and the lower part of the positioning pin is large, the installation platform is arranged below the liquid transferring workbench, one end of the workbench supporting plate supports the workbench, the other end of the workbench supporting plate is connected with the installation platform, the box body of the reaction box is formed by injection molding, magnets are arranged at four corners of the cover, the box body of the reaction box and the cover are tightly matched through the magnets, a through hole for a sample adding needle to pass through is arranged on the cover, a transparent part for observation is arranged on the cover, and the bottom end face of the reaction box is in an arc shape which is slightly convex upwards.
3. The precisely positionable platform of claim 2, wherein: the elastic sampling needle comprises a sampling needle connecting sleeve, a sampling needle fixing seat, a spring, a sampling needle fastening sleeve and a sampling needle, and the lower part of the sampling needle connecting sleeve is in threaded connection with the sampling needle fastening sleeve; the sampling needle connecting sleeve is fixed on the inner wall of the sampling needle fixing seat; the sample adding needle is partially arranged inside the sample adding needle connecting sleeve and the sample adding needle fastening sleeve, the spring sleeve is sleeved on the sample adding needle and is arranged in an inner cavity of the sample adding needle connecting sleeve, the inner cavity of the sample adding needle connecting sleeve is provided with a limiting step for controlling the compression stroke of the spring, and the periphery of the sample adding needle is provided with a step.
4. The precisely positionable platform of claim 3, wherein: the upper end of the sample adding needle fixing seat is provided with a limiting piece which is matched with the photoelectric switch and used for controlling the stroke of the sample adding needle, ensuring that the sample adding needle reaches an appointed station to absorb liquid and add sample, and the sample adding needle fixing seat is connected with the transmission mechanism.
5. A working method of a Western Blot fully automatic workstation according to any one of claims 1 to 4, characterized by comprising the following steps: the manipulator is used for taking and placing the reaction box, and the main control board controls the manipulator.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110668442.3A CN113552365B (en) | 2021-06-16 | 2021-06-16 | Western Blot full-automatic workstation |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110668442.3A CN113552365B (en) | 2021-06-16 | 2021-06-16 | Western Blot full-automatic workstation |
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| CN113552365A true CN113552365A (en) | 2021-10-26 |
| CN113552365B CN113552365B (en) | 2024-07-16 |
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2021
- 2021-06-16 CN CN202110668442.3A patent/CN113552365B/en active Active
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2532790A1 (en) * | 2003-07-18 | 2005-01-27 | Bio-Rad Laboratories, Inc. | System and method for multi-analyte detection |
| CN204160482U (en) * | 2014-05-29 | 2015-02-18 | 深圳市亚特安科技有限公司 | Inertia is relied on automatically to put the manipulator of thing |
| US20190284519A1 (en) * | 2017-09-22 | 2019-09-19 | Euroimmun Medizinische Labordiagnostika Ag | Incubation device and system comprising incubation device and rocking platform |
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| CN113552365B (en) | 2024-07-16 |
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