CN220331080U - Magnetic bead reagent positioner - Google Patents

Abstract

The utility model discloses a magnetic bead reagent positioning device, which belongs to the technical field of sample analysis and comprises a base, a bearing table, a magnetic piece and an elastic component, wherein the bearing table is arranged on the base and can bear a reagent container, and a magnetic bead reagent is contained in the reagent container; the magnetic piece is arranged on the bearing table and can generate a magnetic field so that magnetic beads in the magnetic bead reagent are gathered at the bottom of the reagent container; the elastic component is arranged between the bearing table and the base. When reagent container is placed on the plummer, the magnetic field that magnetic part produced makes the magnetic bead in the magnetic bead reagent gather in reagent container's bottom, be convenient for absorb the liquid in the magnetic bead reagent, reduce the magnetic bead residue in the liquid that is absorbed, when the pipetting, the suction head pushes down can lead to the plummer to move down, elastic component is compressed, buffering through elastic component for the suction head can not the excessive pressure, ensure that the suction head can not strike the damage, with abundant absorption liquid, after the suction head lifts up, the plummer can reset under elastic component's effect.

Description

Magnetic bead reagent positioner

Technical Field

The utility model relates to the technical field of sample analysis, in particular to a magnetic bead reagent positioning device.

Background

Magnetic bead reagents are widely used in the field of sample analysis. The magnetic bead reagent generally comprises magnetic beads, a marker and a buffer solution, wherein the magnetic bead reagent is mostly contained in a container such as an orifice plate or a kit, and when in use, the magnetic bead reagent is sucked by a corresponding reagent suction device and is sent into a corresponding reaction container. The reagent sucking device generally comprises a suction head, wherein the suction head enters a hole of a pore plate to suck liquid in the magnetic bead reagent in a pipetting process, and part of the magnetic beads can be sucked away by the suction head in the pipetting process, so that the magnetic beads are wasted. In order to suck out all the liquid in the hole and suck away the magnetic beads as little as possible, in the pipetting process, the suction head is pressed down to fully suck up the reagent, the suction head can strike the magnetic beads, if the abutting force between the suction head and the magnetic beads is overlarge, the suction head can be damaged, and therefore the suction head cannot be over-pressed, and more residual liquid exists in the hole.

Disclosure of Invention

The utility model aims to provide a magnetic bead reagent positioning device, which is used for solving the technical problem that more liquid remains in a pore plate during pipetting operation in the prior art.

The technical scheme adopted by the utility model is as follows:

a magnetic bead reagent positioning apparatus comprising:

a base;

the bearing table is arranged on the base and can bear a reagent container, and the reagent container contains magnetic bead reagents;

the magnetic piece is arranged on the bearing table and can generate a magnetic field so as to enable magnetic beads in the magnetic bead reagent to be gathered at the bottom of the reagent container;

the elastic component is arranged between the bearing table and the base.

Wherein the magnetic member comprises a permanent magnet.

The permanent magnets are arranged in a plurality of rows and a plurality of columns in a matrix.

Wherein the permanent magnet is cylindrical.

Wherein, the upper edge axis of permanent magnet runs through and is provided with the centre bore.

Wherein, the top of magnetic part is provided with the holding groove, the holding groove supplies the bottom of reagent container inserts.

The elastic assembly comprises a guide post and a spring, the guide post penetrates through the bearing table, the spring is sleeved on the guide post, the spring is clamped between the bearing table and the base, and the bearing table can slide along the guide post.

One end of the guide post is in threaded connection with the base.

The guide post penetrates through the guide hole and is connected with the threaded hole, and part of the spring penetrates through the guide hole.

One end of the guide post, which is far away from the base, is provided with a limiting part, and one side of the bearing table, which is far away from the base, is abutted to the limiting part.

The utility model has the beneficial effects that:

according to the magnetic bead reagent positioning device provided by the utility model, when the reagent container is placed on the bearing table, the magnetic beads in the magnetic bead reagent are gathered at the bottom of the reagent container by the magnetic field generated by the magnetic piece, so that the liquid in the magnetic bead reagent is conveniently sucked, the magnetic bead residues in the sucked liquid are reduced, and as the elastic component is arranged between the bearing table and the base, the bearing table can be moved downwards due to the downward pressing of the suction head in the pipetting process, the elastic component is compressed, the suction head can not be over-pressed through the buffering of the elastic component, the suction head can not be impacted and damaged, so that the liquid is fully sucked, and after the suction is finished, the suction head is lifted, and the bearing table can be reset under the action of the elastic component.

Drawings

FIG. 1 is a schematic diagram of a magnetic bead reagent positioning apparatus according to an embodiment of the present utility model;

FIG. 2 is a front view of a magnetic bead reagent positioning apparatus provided by an embodiment of the present utility model;

FIG. 3 is a cross-sectional view taken along A-A of FIG. 2;

FIG. 4 is an enlarged view at B of FIG. 3;

FIG. 5 is a partial cross-sectional view of a side panel provided by an embodiment of the present utility model;

FIG. 6 is a side view of a magnetic bead reagent positioning apparatus provided by an embodiment of the present utility model;

FIG. 7 is a schematic diagram of an exploded structure of a magnetic bead reagent positioning apparatus according to an embodiment of the present utility model;

fig. 8 is a schematic structural view of a guide post according to an embodiment of the present utility model.

In the figure:

10. a base; 101. a guide hole; 102. a threaded hole; 11. a bottom plate; 12. a side plate; 13. a reinforcing plate;

20. a carrying platform; 201. a step hole; 21. a table body; 22. a bracket; 23. a limiting block;

30. a magnetic member;

40. an elastic component; 41. a guide post; 411. a polish rod section; 412. a threaded section; 413. a limit part; 42. and (3) a spring.

Detailed Description

Embodiments of the present utility model are described in detail below, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to like or similar elements or elements having like or similar functions throughout. The embodiments described below by referring to the drawings are illustrative and intended to explain the present utility model and should not be construed as limiting the utility model.

In the description of the present utility model, unless explicitly stated and limited otherwise, the terms "connected," "connected," and "fixed" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally formed; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communicated with the inside of two elements or the interaction relationship of the two elements. The specific meaning of the above terms in the present utility model will be understood in specific cases by those of ordinary skill in the art.

In the present utility model, unless expressly stated or limited otherwise, a first feature "above" or "below" a second feature may include both the first and second features being in direct contact, as well as the first and second features not being in direct contact but being in contact with each other through additional features therebetween. Moreover, a first feature being "above," "over" and "on" a second feature includes the first feature being directly above and obliquely above the second feature, or simply indicating that the first feature is higher in level than the second feature. The first feature being "under", "below" and "beneath" the second feature includes the first feature being directly under and obliquely below the second feature, or simply means that the first feature is less level than the second feature.

The technical scheme of the utility model is further described below by the specific embodiments with reference to the accompanying drawings.

Referring to fig. 1 to 8, an embodiment of the present utility model provides a magnetic bead reagent positioning device, which includes a base 10, a carrying platform 20, a magnetic member 30 and an elastic component 40, wherein the carrying platform 20 is disposed on the base 10, the carrying platform 20 can carry a reagent container, and a magnetic bead reagent is contained in the reagent container; the magnetic piece 30 is arranged on the bearing table 20, and the magnetic piece 30 can generate a magnetic field so as to enable magnetic beads in the magnetic bead reagent to be gathered at the bottom of the reagent container; the elastic component 40 is disposed between the bearing table 20 and the base 10.

When the reagent container is placed on the plummer 20, the magnetic field that magnetic part 30 produced makes the magnetic bead in the magnetic bead reagent gather in the bottom of reagent container, be convenient for absorb the liquid in the magnetic bead reagent, reduce the magnetic bead residue in the liquid of being absorbed, reduce the magnetic bead extravagant, owing to be provided with elastic component 40 between plummer 20 and the base 10, in the pipetting process, the suction head pushes down can lead to plummer 20 to move down, elastic component 40 is compressed, buffering through elastic component 40 for the suction head can not excessive pressure, ensure that the suction head can not strike the damage, in order fully absorb liquid, after the absorption is accomplished, the suction head lifts up, plummer 20 can reset under elastic component 40's effect.

Wherein the reagent container may be an orifice plate or a kit. Taking the pore plate as an example, the pore plate is provided with a plurality of accommodating holes, and each accommodating hole can accommodate a magnetic bead reagent, and can be a 96-pore plate.

The top end of the magnetic member 30 is provided with a receiving groove into which the bottom of the reagent container is inserted. On one hand, the limiting effect is achieved on the reagent container, so that the reagent container is stable in position; on the other hand, the contact area between the reagent container and the magnetic member 30 is increased, and the effect of adsorbing the magnetic beads is improved. It can be understood that the magnetic beads are free in the magnetic bead reagent, and the magnetic beads are positioned by the adsorption of the magnetic piece 30, so that the magnetic beads are prevented from being sucked away by the suction head, and the magnetic bead waste is reduced.

In the present embodiment, the magnetic member 30 includes a permanent magnet, and can maintain its magnetism for a long period of time with high stability. In other embodiments, the magnetic member 30 comprises an electromagnet, which may be an induction coil in particular, that generates magnetic properties when energized. In other embodiments, the magnetic member 30 includes a permanent magnet and an electromagnet, i.e., a portion of the magnetic member 30 is provided as a permanent magnet and a portion of the magnetic member 30 is provided as an electromagnet.

It will be appreciated that the number, shape and size of the magnetic elements 30 may be set according to actual needs. In this embodiment, a plurality of permanent magnets are provided, and the plurality of permanent magnets are arranged in a matrix. Specifically, a permanent magnet is arranged in each accommodating hole of the corresponding pore plate, so that the magnetic beads of the magnetic bead reagent in each accommodating hole of the pore plate can be adsorbed. In other embodiments, the permanent magnet may be provided in a size that is adapted to the size of the well plate such that each of the magnetic beads of the magnetic bead reagent in the well plate is capable of being adsorbed. It will be appreciated that two or more permanent magnets may be provided, and the area of each permanent magnet arranged on the carrying platform 20 is adapted to the size of the well plate, so that the magnetic beads of the magnetic bead reagent in each accommodating hole of the well plate can be adsorbed.

In this embodiment, the permanent magnet is cylindrical. Because the holding hole on the orifice plate is cylindric in most, consequently set up cylindric with the permanent magnet and can contact with the orifice plate better for the magnetic bead atress in the orifice plate is even.

The permanent magnet is provided with a central hole along the axis. The centre bore can be used as the holding groove, because the bottom structure of other consumptive materials such as dark orifice plate is round bottom or V type end generally, and the centre bore makes the permanent magnet can be inseparabler with orifice plate bottom shape laminating, and the laminating face is bigger, and the distance is closer, then adsorbs the magnetic bead effect better.

The bearing table 20 comprises a table body 21 and a bracket 22 arranged on the table body 21, wherein the bracket 22 comprises a plurality of mounting holes arranged at intervals, and a permanent magnet is arranged in each mounting hole. Specifically, the permanent magnet is adhered to the support 22, glue is disposed in the mounting hole, and one end of the permanent magnet is inserted into the mounting hole and adhered to the support 22 under the action of the glue. When the reagent container is placed on the bearing table 20, the magnetic beads in the magnetic bead reagent are gathered at the bottom of the reagent container by the magnetic field generated by the permanent magnet, so that the magnetic bead reagent is conveniently sucked, and when the reagent container is removed, the permanent magnet is fixed and cannot be carried up by the reagent container, so that the stability is ensured.

The carrying platform 20 further comprises a plurality of limiting blocks 23 arranged on the platform body 21, and the limiting blocks 23 are arranged around the circumference of the support 22. When the reagent container is placed on the bearing table 20, the reagent container is located in the space surrounded by the limiting blocks 23, and the limiting blocks 23 play a role in limiting the reagent container, so that the reagent container is prevented from shifting along the bearing table 20.

The elastic component 40 is disposed between the bearing table 20 and the base 10, and plays a role of buffering. In this embodiment, the elastic component 40 includes a guide post 41 and a spring 42, the guide post 41 is disposed through the bearing platform 20, the spring 42 is sleeved on the guide post 41 and is sandwiched between the bearing platform 20 and the base 10, and the bearing platform 20 can slide along the guide post 41. In the pipetting process, the pipette tip is pushed down and can lead to the plummer 20 to move downwards, the plummer 20 slides downwards along the guide post 41, the spring 42 is compressed, the spring 42 has certain elasticity, and the set length of the spring 42 enables the pipette tip not to compress tightly the spring 42 completely, so the pipette tip can play a buffering role, the pipette tip can not be over-pressed, the pipette tip can not be impacted and damaged, so that the magnetic bead reagent is fully absorbed, reagent residues are reduced, after the absorption is completed, the pipette tip is lifted, and the plummer 20 can be reset under the action of the spring 42.

Wherein, the guide post 41 plays a guiding role on the spring 42, so that the spring 42 is deformed along the extending direction of the guide post 41, and the spring 42 is prevented from being displaced.

In other embodiments, the elastic component 40 may be a gasket or a washer such as rubber, silica gel, etc., taking a rubber pad as an example, when the area of the rubber pad is large, the guiding structure may not be provided, and the structure is simple, and the installation and the disassembly are convenient.

In this embodiment, one end of the guide post 41 is screwed with the base 10, so that the installation and the disassembly are convenient, and the height of the guide post 41 protruding from the base 10 can be adjusted as required, so that the compressed amount of the spring 42 can be adjusted.

Specifically, the base 10 is provided with a guide hole 101 and a screw hole 102 communicating with the guide hole 101, the guide post 41 is inserted into the guide hole 101 and connected to the screw hole 102, and a part of the spring 42 is inserted into the guide hole 101. The guide hole 101 plays a role in positioning the spring 42, so that the position of the spring 42 is stable, and the spring 42 is prevented from tilting.

It will be appreciated that the guide post 41 includes a polish rod section 411 and a threaded section 412, the threaded section 412 being capable of being connected to the threaded bore 102, a portion of the polish rod section 411 being disposed through the guide bore 101, and a portion of the polish rod section 411 protruding from the base 10. In this embodiment, the diameter of the guide hole 101 is larger than that of the threaded hole 102, so that the machining and the production are facilitated.

One end of the guide post 41, which is far away from the base 10, is provided with a limiting part 413, one side of the bearing table 20, which is far away from the base 10, is abutted against the limiting part 413, and the limiting part 413 plays a limiting role on the bearing table 20, so that the bearing table 20 is prevented from sliding off from the guide post 41. In order to ensure the flatness of the surface of the bearing table 20, a stepped hole 201 is provided on the bearing table 20, the guide post 41 is inserted into the stepped hole 201, and the limiting portion 413 is located in the stepped hole 201. Specifically, the guide post 41 may employ an existing contour screw.

In this embodiment, the carrying platform 20 is substantially rectangular, the elastic component 40 includes four guide posts 41, the four guide posts 41 are distributed on four corners of the carrying platform 20, and a spring 42 is sleeved on each guide post 41.

The base 10 supports the carrying table 20. The base 10 includes a bottom plate 11 and two side plates 12 disposed at intervals, the side plates 12 are connected with the bottom plate 11, and the elastic assembly 40 is disposed between the side plates 12 and the table body 21. Specifically, the side plate 12 is vertically connected to the bottom plate 11, the stepped hole 201 is formed in the table body 21, and the guide hole 101 and the screw hole 102 are formed in the side plate 12. The base 10 further includes a reinforcing plate 13, and the reinforcing plate 13 is disposed between the two side plates 12. That is, one end of the reinforcing plate 13 is connected to one side plate 12, and the other end of the reinforcing plate 13 is connected to the other side plate 12.

The magnetic bead reagent positioning device can be used alone or as a quick assembly disassembly standard module to be matched with gene equipment; the magnetic bead reagent positioning device not only can position the magnetic beads in the reagent container during pipetting, but also can be used in the processes of transferring, eluting and washing the magnetic beads.

The above embodiments merely illustrate the basic principle and features of the present utility model, and the present utility model is not limited to the above embodiments, but may be varied and altered without departing from the spirit and scope of the present utility model. The scope of the utility model is defined by the appended claims and equivalents thereof.

Claims (9)

1. A magnetic bead reagent positioning apparatus, comprising:

a base (10);

the bearing table (20) is arranged on the base (10), the bearing table (20) can bear a reagent container, and a magnetic bead reagent is contained in the reagent container;

a magnetic member (30) disposed on the carrying table (20), wherein the magnetic member (30) is capable of generating a magnetic field so as to cause magnetic beads in the magnetic bead reagent to be gathered at the bottom of the reagent container;

an elastic component (40) arranged between the bearing table (20) and the base (10);

the top end of the magnetic piece (30) is provided with a containing groove, and the bottom of the reagent container is inserted into the containing groove.

2. The magnetic bead reagent positioning device according to claim 1, wherein the magnetic member (30) comprises a permanent magnet.

3. The magnetic bead reagent positioning device according to claim 2, wherein a plurality of the permanent magnets are provided, and a plurality of the permanent magnets are arranged in a matrix.

4. The apparatus according to claim 3, wherein the permanent magnet has a cylindrical shape.

5. The magnetic bead reagent positioning apparatus according to claim 4, wherein the permanent magnet is provided with a center hole penetrating along the axis.

6. The magnetic bead reagent positioning device according to any one of claims 1 to 5, wherein the elastic component (40) comprises a guide post (41) and a spring (42), the guide post (41) is arranged on the bearing table (20) in a penetrating manner, the spring (42) is sleeved on the guide post (41), the spring (42) is clamped between the bearing table (20) and the base (10), and the bearing table (20) can slide along the guide post (41).

7. The magnetic bead reagent positioning device according to claim 6, wherein one end of the guide post (41) is screwed with the base (10).

8. The magnetic bead reagent positioning device according to claim 7, wherein the base (10) is provided with a guide hole (101) and a threaded hole (102) communicated with the guide hole (101), the guide column (41) is arranged in the guide hole (101) in a penetrating manner and is connected with the threaded hole (102), and part of the spring (42) is arranged in the guide hole (101) in a penetrating manner.

9. The magnetic bead reagent positioning device according to claim 7, wherein a limiting portion (413) is provided at an end of the guide post (41) away from the base (10), and a side of the carrying table (20) away from the base (10) abuts against the limiting portion (413).