CN109975562A - Chemiluminescence detector and its detection method - Google Patents
Chemiluminescence detector and its detection method Download PDFInfo
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- CN109975562A CN109975562A CN201711465126.6A CN201711465126A CN109975562A CN 109975562 A CN109975562 A CN 109975562A CN 201711465126 A CN201711465126 A CN 201711465126A CN 109975562 A CN109975562 A CN 109975562A
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- G—PHYSICS
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- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
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Abstract
The present invention provides a kind of chemiluminescence detector, comprising: reagent storage means are used for storing reagent；The reagent storage means include at least two reagent storing mechanisms；Reaction unit is operated for carrying reaction vessel and carrying out sample-adding sheet, reagent and incubate；The reaction unit has at least two reagent adding stations；And dispenser；The dispenser includes sample charging mechanism and at least two pipetting devices；Each pipetting device respectively corresponds the reagent storing mechanism and a reagent adding station, and the pipetting device will be in the reaction vessel of the agent transfer in the corresponding reagent storing mechanism to the corresponding reagent adding station；Sample is transferred in the reaction vessel by the sample charging mechanism.While meeting different sample reagent adding type demands, the transfer velocity of reagent is greatly improved, improves the detection speed of chemiluminescence detector.The present invention also provides a kind of detection methods of chemiluminescence detector.
Description
Technical field
The present invention relates to chemiluminescence detection technology fields, more particularly to a kind of chemiluminescence detector and its detection side
Method.
Background technique
Chemiluminescence immunoassay is a kind of vitro detection for being combined antigen-antibody immune response and luminescence-producing reaction
Analytical technology, it is based on Immunology, a variety of to detect by collecting optical signal using luminous marker as tracer signal
Marker has high sensitivity, the advantage that non-specific adsorption is low, accuracy rate is high.With the high speed development of biological medicine equipment,
Realize that the full-automation of chemiluminescence detector has certain condition.
Have become mature medical diagnosis generally, based on the chemiluminescence detector of chemiluminescence immunoassay and set
It is standby.However, universal chemiluminescence detector equipment price is expensive, volume is heavy, power hungry, it is difficult to universal and popularization.And
With the high speed development of biological medicine equipment, realize that the full-automation of chemiluminescence detector has certain condition.
Chemiluminescence detector mainly include reaction cup loading device, sample adding device, incubation reaction device, cleaning device,
Measuring device, control system and software systems.But each section of process of current chemiluminescence detector as plus reaction cup,
Sample-adding this, the processes such as reagent adding and mixing be distributed in assembly line when executing, cooperate traditional reagent adding device that can seriously restrict instrument
The detection speed of device, influences detection efficiency.
Summary of the invention
Based on this, it is necessary to cooperate traditional add in assembly line distribution when executing for each section of process that current sample reacts
The problem of instrument detection speed is restricted caused by reagent device provides a kind of speed of raising agent transfer to guarantee that instrument detects
The chemiluminescence detector of speed, while a kind of detection method using above-mentioned chemiluminescence detector being also provided.
Above-mentioned purpose is achieved through the following technical solutions:
A kind of chemiluminescence detector, comprising:
Reagent storage means are used for storing reagent；The reagent storage means include at least two reagent storing mechanisms；
Reaction unit is operated for carrying reaction vessel and carrying out sample-adding sheet, reagent adding and incubate；The reaction unit
With at least two reagent adding stations；And
Dispenser is used for sample and agent transfer into the reaction vessel；The dispenser includes sample-adding machine
Structure and at least two pipetting devices；Each pipetting device respectively corresponds described in the reagent storing mechanism and one
Reagent adding station, the pipetting device is by the agent transfer in the corresponding reagent storing mechanism to the corresponding reagent adding
In the reaction vessel of station；Sample is transferred in the reaction vessel by the sample charging mechanism.
At least two reagent storing mechanisms include the first reagent storing mechanism and second in one of the embodiments,
Reagent storing mechanism, at least described reagent adding station include the first reagent adding station and the second reagent adding station, at least two institutes
Stating pipetting device includes the first pipetting device and the second pipetting device；
First pipetting device corresponds to the first reagent storing mechanism and the first reagent adding station setting, is used for
By the agent transfer in the first reagent storing mechanism into the corresponding reaction vessel of the first reagent adding station；
Second pipetting device corresponds to the second reagent storing mechanism and the second reagent adding station setting, is used for
By the agent transfer in the second reagent storing mechanism into the corresponding reaction vessel of the second reagent adding station.
The chemiluminescence detector further includes the reaction for conveying the reaction vessel in one of the embodiments,
Container automatic conveying device, the reaction vessel automatic conveying device are located at the side of the reaction unit；
Also there is the chemiluminescence detector new cup to grab cup mechanism, and the new cup grabs cup mechanism and corresponds to the reaction vessel
Automatic conveying device and reaction unit setting, for by the reaction vessel in the reaction vessel automatic conveying device
It is transferred in the reaction unit.
The chemiluminescence detector further includes cleaning device in one of the embodiments, for removing the reaction
Impurity in container；The cleaning device is located at the side of the reaction unit；
Also there is the chemiluminescence detector cleaning to grab cup mechanism, and the cleaning grabs cup mechanism and corresponds to the cleaning device
And the reaction unit setting, for the reaction vessel to be shifted between the reaction unit and the cleaning device.
The chemiluminescence detector further includes measuring device in one of the embodiments, for holding to the reaction
Determinand in device is detected；The measuring device is located at the side of the reaction unit, and adjacent with the cleaning device
Setting；
Also there is the chemiluminescence detector measurement to grab cup mechanism, and the measurement grabs cup mechanism and corresponds to the cleaning device
And the measuring device setting, for the reaction vessel after cleaning in the cleaning device to be transferred to the measuring device
In.
The reaction unit includes buffering disc mechanism and independently of the buffering disc mechanism in one of the embodiments,
Disc mechanism is reacted, the buffering disc mechanism, which accommodates the reaction vessel and executes, adds sample operations, and the reaction disc mechanism accommodates
The reaction vessel simultaneously executes reagent adding, mixing and incubates operation；
Also there is the chemiluminescence detector sample to grab cup mechanism, and the sample grabs cup mechanism and corresponds to the buffer disc machine
Structure and reaction disc mechanism setting, for the reaction vessel on the buffering disc mechanism to be transferred to the reaction tray machine
In structure.
In one of the embodiments, the reaction disc mechanism include in independently operated reaction disc mechanism with react external disk
Mechanism, the outer disc mechanism of the reaction are sheathed on the outside of disc mechanism and coaxial setting in the reaction；The outer disc mechanism of the reaction
It accommodates the reaction vessel and executes reagent adding and mix and operate, disc mechanism accommodates the reaction vessel and executes in the reaction
Incubate operation；
Also there is the chemiluminescence detector incubation to grab cup mechanism, and the incubation grabs cup mechanism and corresponds to the reaction inner disc
Mechanism reacts outer disc mechanism and is arranged with described, for by the reaction vessel, disc mechanism with described to react external disk in the reaction
It is shifted between mechanism.
The first reagent adding station is disposed adjacent with the second reagent adding station in institute in one of the embodiments,
It states on the outer disc mechanism of reaction, the first reagent storing mechanism is symmetrical with the second reagent storing mechanism and is arranged side by side in institute
State the side for reacting outer disc mechanism.
The chemiluminescence detector further includes sample conveying device in one of the embodiments, for sample is defeated
It send to sample position；The sample position is located at the side of the buffering disc mechanism and the corresponding sample charging mechanism setting；It is described
Sample charging mechanism is drawn sample in sample position and is transferred in the reaction vessel of the buffering disc mechanism.
The sample conveying device includes sample storing mechanism and stores machine with the sample in one of the embodiments,
The sample conveying mechanism that structure is detachably connected stores multiple sample racks for being mounted with sample in the sample storing mechanism, described
The sample rack is transported in the sample conveying mechanism by sample storing mechanism, and is delivered to institute by the sample conveying mechanism
State sample position.
The input terminal of the sample conveying mechanism can be with the sample storing mechanism pair in one of the embodiments,
It connects, or is docked with the output end of the sample conveying mechanism of another chemiluminescence detector；
The output end of the sample conveying mechanism can be with the sample conveyer of another chemiluminescence detector
The input terminal of structure docks.
The chemiluminescence detector further includes consumption material box loading device in one of the embodiments, for passing automatically
The defeated consumptive material the storage box for loading fluid suction head, and the consumptive material the storage box can also be delivered to and load position by the consumption material box loading device
It sets；The sample charging mechanism loads the fluid suction head in the " loaded " position, and shifts sample by the fluid suction head.
It is separate to be located at the reaction vessel automatic conveying device for the consumption material box loading device in one of the embodiments,
The buffering disc mechanism side, and the corresponding sample charging mechanism setting.
The chemiluminescence detector further includes pedestal in one of the embodiments, and the pedestal has carrying platform,
The reaction unit, the measuring device, the cleaning device, the dispenser and the reagent storage means are all set in
On the carrying platform of the pedestal.
The chemiluminescence detector further includes control device and fluid path device in one of the embodiments,；The control
Device processed and the reaction unit, the measuring device, the cleaning device, the dispenser and the reagent storage means
Electrical connection, the fluid path device are connect with the dispenser；The pedestal has accommodating space, and the accommodating space is located at institute
The lower section of pedestal is stated, the control device and fluid path device are set in the accommodating space.
A kind of detection method of chemiluminescence detector, includes the following steps:
It is loaded this step；Sample is transferred in reaction vessel；
Reagent adding step；By agent transfer into the reaction vessel；
Mix step；Mixture in the reaction vessel is uniformly mixed；
Incubation step；Incubation operation is carried out to the mixture being uniformly mixed in the reaction vessel；
Separation cleaning step；The impurity in the reaction vessel after removal incubation；
Measuring process；Determinand in the reaction vessel after cleaning is detected.
The detection method further includes following steps in one of the embodiments:
Before the separation cleaning step, the reagent adding step, the mixing that at least one is recycled are repeated
Step and the incubation step；And/or
Before the measuring process, repeat at least one circulation the reagent adding step, the mixing step,
The incubation step and the separation cleaning step.
In one of the embodiments, before the measuring process, the separation cleaning step is to described in after incubation
Impurity in reaction vessel carries out separation cleaning at least once and operates.
After adopting the above technical scheme, the invention has the benefit that
Chemiluminescence detector and its detection method of the invention, when operation, sample charging mechanism has added sample to reaction vessel
After this, pipetting device is by the reaction vessel of the agent transfer in reagent storage means to reaction unit, then, in reaction unit
Reaction vessel execute mix with incubate operate.Examination is realized at least two pipetting devices and the cooperation of at least two reagent storing mechanisms
The transfer of agent greatly improves the transfer velocity of reagent, effectively solves while meeting different sample reagent adding type demands
It has determined to cooperate when each section of process that current sample reacts executes in assembly line distribution and has restricted instrument caused by traditional reagent adding device
Device detects the problem of speed, accelerates agent transfer efficiency, and then improve the detection speed of chemiluminescence detector, and then guarantees
Detection efficiency.
Detailed description of the invention
Fig. 1 is the schematic top plan view of the chemiluminescence detector of one embodiment of the invention；
Fig. 2 is the first reagent storing mechanism schematic front view in chemiluminescence detector shown in Fig. 1；
Fig. 3 is the schematic front view of structure of controlling temperature in the first reagent storing mechanism shown in Fig. 2；
Fig. 4 is cooling assembly structure schematic side view in structure of controlling temperature shown in Fig. 3；
Wherein:
10- pedestal；
11- reagent storage means；
111- the first reagent storing mechanism；
1111- agent bin ontology；
1112- structure of controlling temperature；
11121- heat dissipation wind channel；
111211- primary air separating duct；
The air duct 111212- box；1112121- extension；
Air duct among 111213-；
Air duct after 111214-；
111215- blower；
11122- cooling assembly；
111221- radiator；1112211- heat sink；1112212- heat dissipation grid；
111222- bottom thermal transfer plate；
111223- cooling piece；
111224- thermal insulation cotton；1112241- stores through-hole；
111225- heat conductive pad；
111226- screw；
111227- spring washer；
111228- heat insulating mattress；
111229- overtemperature protector；
111230- tabletting；
111231- temperature sensor；
112- the second reagent storing mechanism；
12- dispenser；
121- sample charging mechanism；
122- pipetting device；
13- reaction unit；
131- buffers disc mechanism；
132- reacts outer disc mechanism；
133- reacts interior disc mechanism；
14- cleaning device；
15- measuring device；
16- grabs cup device；
The new cup of 161- grabs cup mechanism；
162- sample grabs cup mechanism；
163- incubation grabs cup mechanism；
Cup mechanism is grabbed in 164- cleaning；
Cup mechanism is grabbed in 165- measurement；
17- reaction vessel automatic conveying device；
18- consumption material box loading device；
19- sample conveying device；
191- sample storing mechanism；
192- sample conveying mechanism；
20- reagent needle cleans component.
Specific embodiment
In order to make the objectives, technical solutions, and advantages of the present invention clearer, by the following examples, it and combines attached
Figure, is further elaborated chemiluminescence detector and its detection method of the invention.It should be appreciated that described herein
Specific examples are only used to explain the present invention, is not intended to limit the present invention.
It should be noted that being herein component institute serialization number itself, such as " first ", " second " etc., it is only used for distinguishing
Described object does not have any sequence or art-recognized meanings.And " connection ", " connection " described in the application, unless otherwise instructed,
It include directly or indirectly connecting (connection).In the description of the present invention, it is to be understood that, term " on ", "lower", " preceding ",
The side of the instructions such as " rear ", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outside", " clockwise ", " counterclockwise "
Position or positional relationship are to be based on the orientation or positional relationship shown in the drawings, and are merely for convenience of description of the present invention and simplification of the description,
Rather than the device or element of indication or suggestion meaning must have a particular orientation, be constructed and operated in a specific orientation, because
This is not considered as limiting the invention.
In the present invention unless specifically defined or limited otherwise, fisrt feature in the second feature " on " or " down " can be with
It is that the first and second features directly contact or the first and second features pass through intermediary mediate contact.Moreover, fisrt feature exists
Second feature " on ", " top " and " above " but fisrt feature be directly above or diagonally above the second feature, or be merely representative of
First feature horizontal height is higher than second feature.Fisrt feature can be under the second feature " below ", " below " and " below "
One feature is directly under or diagonally below the second feature, or is merely representative of first feature horizontal height less than second feature.
Referring to Fig. 1, the present invention provides a kind of chemiluminescence detector, which can carry out sample
Processing, and to treated, sample carries out analysis detection, obtains corresponding testing result, satisfies the use demand.It needs to illustrate
It is that the specific type of sample to be measured is unrestricted, in some embodiments, sample to be measured includes solid sample or liquid
Sample.Further liquid sample includes but is not limited to blood sample.Chemiluminescence detector of the invention can meet not
While with sample reagent adding type demand, the transfer velocity of reagent is greatly improved, the inspection of chemiluminescence detector is improved
Degree of testing the speed, and then guarantee detection efficiency.
In the present invention, chemiluminescence detector includes reagent storage means 11, reaction unit 13 and dispenser 12.Examination
Agent storage device 11 is used for storing reagent, can store required various reagents when pattern detection, examination needed for facilitating selection
Agent improves the efficiency for drawing reagent.It is understood that reagent storage means 11 can store plurality of reagents, reagent storage dress
Setting 11 also there is cold storage function to realize the preservation of reagent for storing the reagent of low temperature.Reaction unit 13 holds for carrying reaction
Device simultaneously carries out sample-adding sheet, reagent adding and incubates operation, and the sample in such reaction vessel can sufficiently be reacted with reagent, so that
It obtains sample and reaches optimal reaction condition with reacting for reagent, facilitate the detection of determinand parameter.Dispenser 12 is used for suction
Sample or reagent are added in corresponding reaction vessel by sample or reagent with realizing.
Moreover, chemiluminescence detector further includes cleaning device 14 and measuring device 15.Cleaning device 14 is for removing temperature
The impurity in reaction vessel after educating, measuring device 15 is for detecting the determinand in reaction vessel.It is understood that
It is that reaction unit 13 can carry out incubation operation to the mixture in reaction vessel, so that sample is sufficiently reacted and tied with reagent
It closes, and impurity then needs to be removed in reaction vessel, in order to avoid influence the accuracy of 15 testing result of measuring device.Therefore, it reacts
Reaction vessel in device 13 separate with impurity clear to the determinand in reaction vessel after incubation, by cleaning device 14
It washes, to remove impurity in reaction vessel, so that leaving determinand in reaction vessel；Then, measuring device 15 is again to reaction vessel
In determinand detected, to obtain every detection parameters of sample.
Understanding to sample and each stage name of reagent for convenience, herein to the title of sample and reagent each stage
Be described in detail: the sample in reaction vessel is known as mixture after mixing with reagent, and reaction unit 13 can be in reaction vessel
Mixture carries out incubation operation, so that sample is sufficiently reacted with reagent, at this point, the substance in reaction vessel is determinand and miscellaneous
Matter.Wherein, mixture refers to that ratio, the concentration of the substance formed after sample is mixed with reagent and sample and reagent are unrelated, herein
Referred to as mixture.Mixture after reaction is presented in a manner of determinand and impurity in the reaction vessel.Impurity can be inabundant
The substance of reaction, or the side reaction product that side reaction generates occurs, can also be detected for other influences measuring device 15
Substance etc., or be above-mentioned at least two composition.Cleaning device 14 removes the impurity in reaction vessel, measuring device
Determinand in 15 detection reaction vessels, to obtain the parameters of sample.
Further, chemiluminescence detector further includes grabbing cup device 16, grabs cup device 16 and exists for realizing reaction vessel
It is shifted between reaction unit 13, cleaning device 14 and measuring device 15, so that reaction vessel at various locations and executes correspondence
Operation, realize the detection that automatically analyzes of sample, improve operating efficiency.
At runtime, reaction unit 13 carries reaction vessel to chemiluminescence detector, and dispenser 12 is drawn sample and turned
It moves on in reaction vessel, dispenser 12 can also draw reagent in reagent storage means 11 and be transferred in reaction vessel；With
Afterwards, reaction unit 13 carries out mixing operation to the mixture in reaction vessel, so that the sample in reaction vessel and reagent are i.e. mixed
Close object be uniformly mixed, reaction unit 13 carries out incubation operation to the mixture in reaction vessel after mixing so that sample with
Reagent sufficiently reacts.It grabs cup device 16 reaction vessel after reaction is transferred in cleaning device 14, be gone by cleaning device 14
Except impurity in reaction vessel.After the completion of cleaning, grabs cup device 16 and reaction vessel is transferred in measuring device 15 again, pass through measurement
Device 15 detects determinand in reaction vessel, to obtain every detection parameters of sample, satisfies the use demand.
Specifically, in the present invention, reagent storage means 11 include at least two reagent storing mechanisms.Reaction unit 13 has
There are at least two reagent adding stations.Dispenser 12 includes sample charging mechanism 121 and at least two pipetting devices 122.Sample charging mechanism
121 are transferred to sample in reaction vessel.Each pipetting device 122 respectively corresponds a reagent storing mechanism and an additional examination
Agent station, pipetting device 122 hold the reaction of the agent transfer in corresponding reagent storing mechanism to corresponding reagent adding station
In device.
At runtime, at least two pipetting devices 122 can be respectively by corresponding reagent storing mechanism for chemiluminescence detector
In reagent into the reaction vessel of reaction unit 13 transfering reagent, can be at least two pipetting devices 122 respectively by reagent
Be transferred at least two reaction vessels, be also possible at least two pipetting devices 122 add reagent on respectively it is same anti-
It answers in container, can also be that at least two pipetting devices 122 add reagent in reaction vessel several times or once.In this way,
It enables to reagent adding operation at least twice while carrying out or alternately, shortening the time of reagent adding, greatly improve chemistry
The operational efficiency of luminometer.
Moreover, because different samples need to add different reagents in reaction, existed by least two pipetting devices 122
Corresponding reagent adding station transfering reagent can satisfy the use demand of different samples.Each sample can be according to actual use demand
It selects a reagent adding station wherein or is added reagent operation at two reagent adding stations.122 energy of pipetting device
It is enough to draw reagent in corresponding reagent storing mechanism, and be moved at corresponding reagent adding station into reaction vessel and add examination
Agent.It is understood that since mobile needs of the pipetting device 122 between reagent storing mechanism and reagent adding station are certain
Time, i.e. pipetting device 122 are moved at reagent storing mechanism after absorption reagent, and retransferring back reagent adding station addition reagent needs
The time wanted is respectively greater than the operating time of transfer reaction container and mixes the operating time, and at least two pipetting devices are arranged
122 at least two reagent adding stations of cooperation can guarantee that pipetting device 122 can be completed to draw reagent and add in one cycle
Reagent adding operation enables reaction vessel to complete reagent adding operation at least one reagent adding station.Here a cycle
Refer to that reaction unit 13 moves the primary time.Each step such as reagent adding in this way on reaction unit 13 mixes, takes cup and put cup
Equal operations can carry out simultaneously, can shorten the time that each step waits, then shorten the operating time of reaction unit 13, with
The treatment effeciency of reaction unit 13 is improved, and then improves the speed of service of complete machine.Optionally, at least two reagent storing mechanisms can
To be structure as a whole, at least two pipetting devices 122 can select required reagent in reagent storing mechanism, and corresponding
Reagent adding station is added in reaction vessel.
Further, at least two reagent storing mechanisms include that the first reagent storing mechanism 111 and the second reagent store machine
Structure 112, at least reagent adding station include the first reagent adding station and the second reagent adding station, at least two pipetting devices 122 packet
Include the first pipetting device and the second pipetting device.The corresponding first reagent storing mechanism 111 of first pipetting device and described first plus
Reactant station setting, for by the agent transfer in the first reagent storing mechanism 111 to the corresponding reaction of the first reagent adding station
In container.The corresponding second reagent storing mechanism 112 of second pipetting device and the setting of the second reagent adding station, are used for the second reagent
Agent transfer in storing mechanism 112 is into the corresponding reaction vessel of the second reagent adding station.
That is, chemiluminescence detector of the invention is stored by the first reagent storing mechanism 111 and the second reagent
Mechanism 112 is for reagent needed for storing pattern detection, it is to be understood that the first reagent storing mechanism 111 and the second reagent
In storing mechanism 112 type of storing reagent according to complete machine use demand be arranged, can different or part it is identical, can also be complete
It is exactly the same.First pipetting device and the second pipetting device respectively correspond the first reagent storing mechanism 111 and the second reagent storage machine
Structure 112 is arranged, and also respectively corresponds the first reagent adding station and the second reagent adding station is arranged.First pipetting device and the second liquid relief
The mode that mechanism matches transfering reagent can be improved the speed of transfering reagent, and then improve the operational efficiency of complete machine.First moves
Reagent is drawn in the first reagent storing mechanism 111 for liquid mechanism and to be transferred to reaction unit 13 in the first reagent adding station corresponding
In reaction vessel, the second pipetting device is drawn reagent in the second reagent storing mechanism 112 and is shifted in the second reagent adding station
Into the corresponding reaction vessel of reaction unit 13, it can be avoided the first pipetting device and the second pipetting device in this way and drawing reagent
It is interfered when with addition reagent, guarantees that reagent adding process operation is accurate and reliable, and then guarantee that chemiluminescence detector is normally transported
Row.
Preferably, the first reagent adding station is disposed adjacent with the second reagent adding station, correspondingly, the first reagent storing mechanism
111 and second reagent storing mechanism 112 be symmetrically disposed on the side of reaction unit 13, and respectively correspond the first reagent adding station with
The setting of second reagent adding station, the corresponding first reagent adding station of the first pipetting device and the first reagent storing mechanism 111 are arranged, the
The corresponding second reagent adding station of two pipetting devices and the setting of the second reagent storing mechanism 112.The first liquid relief machine can be shortened in this way
Motion path when structure and the second pipetting device transfering reagent further shortens the time consumed by transfering reagent, improves operation
Efficiency.Moreover, the first reagent storing mechanism 111 and the second reagent storing mechanism 112 are arranged side by side, it in this way can be to reduction reagent
11 the space occupied of storage device, so that chemiluminescence detector complete machine is rationally distributed, to reduce package size.In the present embodiment
Only two pipetting devices 122 are cooperated to add reagent at two reagent adding stations with two reagent storing mechanisms, of the invention
In other embodiments, the quantity of reagent storing mechanism, reagent adding station and pipetting device 122 is not limited to two, may be used also
Think more, to meet the use demand of distinct device.
In addition, using double reagent storehouse i.e. the first reagent storing mechanism 111 and the second reagent storing mechanism 112 and the first liquid relief
The selection that additional amount and type to reagent are capable of in the cooperation of mechanism and the second pipetting device is more flexible, the first pipetting device and the
One species or different types of reagent can be added in two pipetting devices, and/or, the first pipetting device and the second pipetting device
It can several times or single adds reagent into reaction vessel.When single adds reagent, i.e. the first pipetting device and the second liquid relief
After a mechanism reagent of every addition, need to again move at the first reagent storing mechanism 111 and the second reagent storing mechanism 112
Draw reagent.It, can be with after the first pipetting device and/or the second pipetting device draw a reagent when adding reagent several times
Divide and be added in same reaction vessel or multiple reaction vessels several times, the return of pipetting device 122 can be reduced in this way and drawn again
The time of reagent consumption, improve treatment effeciency.Moreover, the first pipetting device and the second pipetting device can also by reagent of the same race or
Reagent not of the same race is respectively added in the same reaction vessel or different reaction vessels.In this way, operator can basis
The operating condition of complete machine is adjusted the first pipetting device and the second pipetting device transfering reagent, to adapt to different uses
Demand.
At runtime, sample charging mechanism 121 is added chemiluminescence detector of the invention to the reaction vessel of reaction unit 13
After complete sample, pipetting device 122 is by the reaction for adding sample of the agent transfer in reagent storage means 11 to reaction unit 13
In container, then, the reaction vessel in reaction unit 13, which executes, to be mixed and incubates operation.Reaction vessel after incubation is cleaning again
Purged of impurities etc. in device 14, then again detects determinand by measuring device 15.By the first pipetting device and
The transfer of reagent is realized in second pipetting device and the cooperation of the first reagent storing mechanism 111 and the second reagent storing mechanism 112, full
While foot difference sample reagent adding type demand, the transfer velocity of reagent is greatly improved, current sample is efficiently solved
Reaction each section of process execute when holding time and occupy larger space caused by long operational time problem, improve chemistry
The detection speed of luminometer, and then guarantee detection efficiency.
Since the first reagent storing mechanism 111 is identical with the structure of the second reagent storing mechanism 112, here, only with
Illustrate for the specific structure of first reagent storing mechanism 111.As shown in Figures 2 and 3, the first reagent storing mechanism 111 includes
Agent bin ontology 1111 and structure of controlling temperature 1112, agent bin ontology 1111 are used for storing reagent, and structure of controlling temperature 1112 is for controlling
Environment temperature in agent bin ontology 1111.Moreover, reagent is embarked on journey into agent bin ontology 1111 by reagent storage container
Column arrangement, the first pipetting device move in the horizontal plane relative to agent bin ontology 1111 and do on vertical plane lifting fortune
It is dynamic, realize the absorption of reagent.Certainly, in other embodiments of the invention, examination is can also be used in the first reagent storing mechanism 111
Agent disk carries reagent, drives reagent to turn to preset station by reagent carrier, the first pipetting device is inhaled in default station
Take reagent.
Specifically, structure of controlling temperature 1112 is set to the lower section of agent bin ontology 1111, structure of controlling temperature 1112 is for adjusting examination
Environment temperature in agent storehouse ontology 1111, so that the environment temperature position in agent bin ontology 1111 is between 2 DEG C -8 DEG C；Temperature control
Structure 1112 includes cooling assembly 11122 and heat dissipation wind channel 11121, and heat dissipation wind channel 11121 includes air duct box 111212 and blower
111215, air duct box 111212 is set to the middle part of heat dissipation wind channel 11121, and cooling assembly 11122 has radiator 111221, dissipates
Hot device 111221 is set in the inner cavity of air duct box 111212, and blower 111215 is set to air duct box in heat dissipation wind channel 11121
111212 along the downstream of gas flow direction.
Further, heat dissipation wind channel 11121 further includes primary air separating duct 111211, intermediate air duct 111213 and rear air duct
111214, primary air separating duct 111211 is set to air duct box 111212 along the front end of gas flow direction, and primary air separating duct 111211 into
Air port is arranged downward, and opening, which is conducive to gas, downward can smoothly enter into primary air separating duct 111211, can increase heat dissipation wind channel
The stability that gas flows in 11121, intermediate air duct 111213 are set to air duct box 111212 along the rear end of gas flow direction,
The one end in air duct 111214 is fixedly connected with intermediate air duct 111213 far from one end of air duct box 111212 afterwards, and blower 111215 is set
It is placed in the one end of rear air duct 111214 far from intermediate air duct 111213.Imply that primary air separating duct 111211, air duct box 111212, intermediate wind
It is sequentially connected between road 111213, rear air duct 111214 for head and the tail, forms a longer air duct, cooling assembly 11122 has
Radiator 111221, radiator 111221 are set in the inner cavity of air duct box 111212, and blower 111215 is set to rear air duct
111214 one end far from intermediate air duct 111213；As a kind of achievable embodiment, primary air separating duct 111211, air duct box
111212, it can realize that head and the tail sequentially connect by flanged joint or welding between intermediate air duct 111213, rear air duct 111214
It connects, in the present embodiment, is sequentially connected with each section of air duct head and the tail using flange；Further, can also be according to actual condition, it will be intermediate
Air duct 111213 is designed as arc, so that the arrangement form of heat dissipation wind channel 11121 adapts to actual condition；Further, air duct box
111212 top has opening, and radiator 111221 is set to air duct box 111212 by the opening of 111212 top of air duct box
In, and it is equipped with sealing device simultaneously；The area of section of air duct box 111212 can be greater than primary air separating duct 111211 connected to it and
The section in intermediate air duct 111213.
In another embodiment, rear air duct 111214 is arranged in close to the one of intermediate air duct 111213 in blower 111215
End.
Above-mentioned first reagent storing mechanism 111, by radiator 111221 be set to heat dissipation wind channel 11121 middle section and
Blower 111215 is set to air duct box 111212 along the downstream of air-flow direction in heat dissipation wind channel 11121, utilizes blower
111215 swabbing action accelerates the flowing of air in heat dissipation wind channel 11121, keeps air-flow more stable；And radiator 111221 is sealing
Forced heat-exchanging in closed chamber body, further improves heat exchange efficiency.
In one embodiment, intermediate air duct 111213 is with the cross section in rear air duct 111214 along far from air duct box 111212
Direction become larger；The air duct that cross section becomes larger is conducive in the attached of the lesser close air duct box 111212 of cross-sectional area
Close to generate the faster gas of flow velocity, the air-flow be conducive in air duct box 111212 quickly flows.
In one embodiment, as shown in figure 4, cooling assembly 11122 further includes bottom thermal transfer plate 111222 and refrigeration
Piece 111223, cooling piece 111223 have cold and hot end, whole flaky, the laminar cooling piece of cooling piece 111223
111223 side is cold end, and the other side opposite with cold end is hot end；Bottom thermal transfer plate 111222 is fixedly installed on agent bin
1111 bottom of ontology, side and 1111 bottom of agent bin ontology of bottom thermal transfer plate 111222 fit, bottom thermal transfer plate
111222 other side and the cold end of cooling piece 111223 fit；Radiator 111221 has heat sink 1112211 and sets
It is placed in the heat dissipation grid of 1112211 side of heat sink, heat dissipation grid is set in air duct box 111212, and heat dissipation grid includes several
Cooling fin, cooling fin heat sink 1112211 side interval, be arranged in parallel, the extending directions of several cooling fins and air duct box
Gas flows to identical, the hot end phase of side of the heat sink 1112211 far from heat dissipation grid and cooling piece 111223 in 111212
Fitting；Heat sink 1112211 is fixedly installed on bottom thermal transfer plate 111222；
Optionally, bottom thermal transfer plate 111222 is fixedly installed on the outer bottom of agent bin ontology 1111, bottom thermal transfer plate
111222 side and the outer bottom of agent bin ontology 1111 fit, alternatively, bottom thermal transfer plate 111222 and agent bin ontology
1111 bottom surface or side are integral type.
As an embodiment, bottom thermal transfer plate 111222 can be fixed by way of bolt or screw 111226
It, further, can also be in the outer bottom and bottom thermal transfer plate of agent bin ontology 1111 in the outer bottom of agent bin ontology 1111
The protrusion being mutually adapted and groove are opened up on 111222 end face, to increase heat exchange area, further increase agent bin ontology
1111 heat exchange efficiency；In the present embodiment, the quantity of cooling piece 111223 is six, and bottom thermal transfer plate 111222 is rectangle
Plate, six cooling pieces 111223 arrangement form in two rows of three in bottom thermal transfer plate 111222；The heat of cooling piece 111223
It holds and fits with the heat sink 1112211 of radiator 111221, heat sink 1112211 passes through the heat dissipation grid being fixed thereon and increases
Add heat exchange area, improves radiating efficiency；
In one embodiment, cooling assembly 11122 further includes thermal insulation cotton 111224, and thermal insulation cotton 111224 is sheet, every
Warm cotton 111224 is located between bottom thermal transfer plate 111222 and heat sink 1112211, has on the end face of thermal insulation cotton 111224
Through-hole 1112241 is stored, the section of storage through-hole 1112241 is adapted with the outer rim of cooling piece 111223, cooling piece 111223
It is set in storage through-hole 1112241；Between the cold end and bottom thermal transfer plate 111222 of cooling piece 111223 and/or cooling piece
Heat conductive pad 111225, in a specific embodiment, cooling piece are provided between 111223 hot end and heat sink 1112211
111223 both ends are provided with heat conductive pad 111225；Thermal insulation cotton 111224 is located in bottom thermal transfer plate 111222 and heat sink
Between 1112211, the heat transmitting between bottom thermal transfer plate 111222 and heat sink 1112211 is effectively prevented, in cooling piece
111223 both ends are sticked heat conductive pad 111225 respectively, can increase cooling piece 111223 and bottom thermal transfer plate 111222 and heat sink
Heat transmitting between 1112211.Further, the material of thermal insulation cotton 111224 can be inorganic asbestos board, by cooling piece 111223
Electric wire be set between thermal insulation cotton 111224 and bottom thermal transfer plate 111222 so that electric wire uses in safe temperature；It is thermally conductive
Pad 111225 can be the good sheet metal or heat-conducting silicone grease of thermal conductivity；It in another embodiment, can be good with thermal insulation
Foamed board replace heat insulation foam.
In one embodiment, heat sink 1112211 is fixedly installed on bottom thermal transfer plate 111222 by screw 111226,
Spring washer 111227 and heat insulating mattress 111228 are equipped on screw 111226, heat insulating mattress 111228 and spring washer 111227 are set to heat dissipation
Between plate 1112211 and bottom thermal transfer plate 111222, screw 111226 also passes through and is located in bottom thermal transfer plate 111222 and heat dissipation
Thermal insulation cotton 111224 between plate 1112211, the fixed mode of screw 111226 is easier to realize, and cost is relatively low, screw
111226 be standard component, also be can be mass-produced, interchangeability is higher；Spring washer 111227 is worn on screw 111226 and by spring washer
111227 are set between heat sink 1112211 and bottom thermal transfer plate 111222, are conducive to the distance of flexible adjustment between the two,
It is unlikely to damage heat sink 1112211 or bottom thermal transfer plate 111222 or both because the fastening force of screw 111226 is too big
Between institute's sandwiched part；The presence of heat insulating mattress 111228 further reduced through the screw on thermal insulation cotton 111224
The heat of 111226 holes transmitting.It in another embodiment, can also be in such a way that bolts and nuts cooperate by heat sink
1112211 are fixedly installed on bottom thermal transfer plate 111222.
In one embodiment, the side wall of air duct box 111212 has extension 1112121, and extension 1112121 is aweather
Road box 111212 extends far from the side of agent bin ontology 1111, and extension 1112121 is in rectangular slab, radiates in heat dissipation grid
The temperature of Cheng Zhong, air duct box 111212 itself inevitably increase, and the gas temperature contacted with air duct box 111212 also can
It increases, extension 1112121 can make the box 111212 raised gas of bottom outside temperature in air duct under air duct box 111212
Fang Liudong prevents the raised gas of temperature from flowing to agent bin ontology 1111 so that extension 1112121 be made to play heat-blocking action
Nearby influence detection work.
In one embodiment, cooling assembly 11122 further includes overtemperature protector 111229 and tabletting 111230, excess temperature
Protector 111229 is set to heat sink 1112211 by tabletting 111230, overtemperature protector 111229 can real-time monitoring dissipate
The temperature of hot plate 1112211 and the control system for feeding back to chemiluminescence detector avoid the temperature mistake of heat sink 1112211
Height, when necessary, the control system of chemiluminescence detector can control cooling piece 111223 to temporarily cease work, further, chemistry
The control system of luminometer can also adjust in real time blower 111215 according to the temperature data that overtemperature protector 111229 is fed back
Revolving speed.
In one embodiment, cooling assembly 11122 further includes temperature sensor 111231, and temperature sensor 111231 is set
Be placed in bottom thermal transfer plate 111222, temperature sensor 111231 can real-time monitoring bottom thermal transfer plate 111222 real time temperature,
It is exactly the temperature of agent bin ontology 1111, and monitoring data is fed back to the control system of chemiluminescence detector, so as to
The operating condition of the structure of controlling temperature 1112 of one reagent storing mechanism 111 is adjusted in real time.
As shown in Figure 1 and Figure 4, the first pipetting device is identical with the structure of the second pipetting device, here, only with first
Illustrate for the specific structure of pipetting device.First pipetting device includes X-axis moving parts, Y-axis moving parts, Z axis exercise group
Part and liquid relief component, X-axis moving parts, Y-axis moving parts and Z axis moving parts drive liquid relief component do X-direction movement,
The movement of Y direction and the movement of Z-direction, so that liquid relief component can move in the first reagent storing mechanism 111
Draw reagent, moreover it is possible to move to the first reagent adding station addition reagent.It is understood that X-axis moving parts, Y-axis exercise group
Part and Z axis moving parts are all made of the transmitting of the realizations campaign such as synchronous band structure, and using motor as power source.Moreover, moving
There is reagent needle in liquid component, draw or be discharged reagent by reagent needle.Also, liquid relief component also uses syringe or column
Reagent is quantitatively drawn in the structures realization such as plug pump.Certainly, in other embodiments of the invention, the first pipetting device be can also be used
Rotating manner makes liquid relief component draw reagent or addition reagent, at this point, the first pipetting device uses synchronous belt structure control
The rotation of liquid relief component.Further, chemiluminescence detector further includes first for cleaning the reagent needle of the first pipetting device
Reagent needle cleans component 20, and the first reagent needle cleaning component 20 is set in the first reagent storing mechanism 111.First pipetting device
Before drawing reagent in the first reagent storing mechanism 111, needs to clean component 20 by the first reagent needle and be cleaned, to keep away
Exempt from cross contamination.
Referring to Fig. 1, as an embodiment, chemiluminescence detector further includes the reaction for conveying reaction vessel
Container automatic conveying device 17.Reaction vessel automatic conveying device 17 for conveying reaction vessel, with realize reaction vessel from
Dynamic conveying, improves transfer efficiency.Certainly, in the other embodiments of invention, reaction vessel automatic conveying device 17 can also be with
It is replaced, i.e., reaction vessel automatic conveying device 17 is not used to convey reaction vessel, reaction vessel can be placed directly into reaction
In device 13.Preferably, the reaction vessel that reaction vessel automatic conveying device 17 conveys is usually medical disposable material, certainly, instead
Answer container that can also be recovered recycling.Optionally, when reaction vessel reuses, reaction vessel can not also be used to pass automatically
Defeated device 17 conveys reaction vessel.It is understood that reaction vessel refers to the consumption for carrying and being able to carry out pattern detection analysis
Material, such as reaction cup, test tube, sample slide, sample tube.In the present embodiment, reaction vessel refers to reaction cup, and reaction vessel is certainly
Dynamic transmitting device 17 usually transmits reaction vessel box, carries arranged in column reaction vessel of embarking on journey in reaction vessel box.Moreover, anti-
It answers the shape of container unrestricted in principle, can be rectangular, round or other shapes, as long as having on reaction vessel box
Ear facilitates reaction vessel automatic conveying device 17 to transmit.
Specifically, reaction vessel automatic conveying device 17 includes reaction vessel storing mechanism and reaction vessel hoisting mechanism.
Reaction vessel storing mechanism is used to load and store multiple reaction vessel boxes, and reaction vessel hoisting mechanism is anti-for storing and being promoted
Container is answered, to realize the conveying of reaction vessel.Reaction vessel hoisting mechanism is located at the top of reaction vessel storing mechanism, reaction
Container storage mechanism can convey reaction vessel box to reaction vessel hoisting mechanism, and reaction vessel hoisting mechanism can pick up reaction
Container is promoted to secondary top layer.Moreover, reaction vessel storing mechanism can be transmitted respectively with reaction vessel hoisting mechanism reacts appearance
Device box.Reaction vessel hoisting mechanism and reaction vessel storing mechanism can independent operating, and parallel transmission reaction vessel respectively.This
Sample, after the reaction vessel box in reaction vessel storing mechanism is partly or entirely transported to reaction vessel hoisting mechanism, Ke Yixiang
Reaction vessel box is loaded in reaction vessel storing mechanism, so that reaction vessel hoisting mechanism continuance lifting reaction vessel box, is realized
The continuous load of reaction vessel box improves whole efficiency；It also will not influence reaction vessel hoisting mechanism transmission reaction vessel box.And
And reaction vessel storing mechanism can also be docked and be separated with reaction vessel hoisting mechanism, be facilitated to reaction vessel storing mechanism
Middle loading reaction vessel box.
Further, the reaction vessel box in reaction vessel storing mechanism is arranged using overlapped way, multiple reactions
Container layering is supported in reaction vessel hoisting mechanism.It can be realized the massive store of reaction vessel box in this way, maximize
Reduction occupy the placement position of instrument and then reduce chemistry hair so that reaction vessel automatic conveying device 17 is compact-sized
The overall dimensions of optical detector.Optionally, overlapped way storage reaction container can also be used in reaction vessel hoisting mechanism.Specifically
, reaction vessel storing mechanism includes the storage holding unit for storing transmission structure and being set on storage transmission structure, storage support
The reaction vessel box that multiple stackings are placed is carried in the portion of rising, and drives storage holding unit to move up and down by depositing transmission structure, it is real
The promotion of existing reaction vessel box.Optionally, storage transmission structure can using synchronous belt wheel structure and/or gear & rack structure etc.
The structure for realizing elevating movement stores the power source of transmission structure using motor etc., and reaction vessel storing mechanism also uses frame
Structure encloses the memory space for being set as reaction vessel box, is not described in detail one by one herein.Reaction vessel hoisting mechanism includes promoting transmission knot
Structure and it is set to the multipair promotion holding unit being oppositely arranged promoted on transmission structure, carrying one is instead in each pair of promotions holding unit
Container is answered, drives promotion holding unit to move up and down by promoting defeated structure, realizes the promotion of reaction vessel box.Optionally,
Promoting transmission structure can be realized the structure of rotary and lifting movement using synchronous belt wheel structure etc., store the power source of transmission structure
Using motor etc. and by the transmitting of the realizations campaigns such as gear, synchronous band structure, reaction vessel hoisting mechanism also uses frame knot
Structure encloses the room for promotion for being set as reaction vessel box, is not described in detail one by one herein.
Chemiluminescence detector grabs what cup device 16 was transmitted in predeterminated position crawl reaction vessel automatic conveying device 17
Reaction vessel in reaction vessel box, specifically: when transmitting reaction vessel box to predeterminated position, reaction vessel hoisting mechanism is upward
The reaction vessel box of secondary top layer is promoted the top layer to reaction vessel box hoisting mechanism by one layer of movement, and reaction vessel transmits automatically
The reaction vessel box of reaction vessel hoisting mechanism top layer is delivered to predeterminated position again by device 17.In this way, grabbing cup device 16 pre-
If position grabs the reaction vessel in reaction vessel box and is transferred in reaction unit 13.
Optionally, reaction vessel automatic conveying device 17 further includes delivery device.Delivery device is set to reaction vessel and mentions
It rises in mechanism, the reaction vessel box of reaction vessel hoisting mechanism top layer can be pushed to predeterminated position by delivery device.Pusher
Structure is released reaction vessel box using the structure of the linear movement outputs such as sliding-rail sliding structure or synchronous band structure, and uses motor
Deng as power source.
Again optionally, reaction vessel automatic conveying device 17 further includes reaction vessel recovering mechanism.Reaction vessel recycling machine
Structure is located at the lower section of predeterminated position, and reaction vessel recovering mechanism can recycle predetermined position and use the reaction vessel box completed.
Reaction vessel recovering mechanism is for realizing that the recycling of reaction vessel box and storage, the reaction vessel box after use can be conveyed
Into reaction vessel recovering mechanism, the recycling and storage of reaction vessel box are realized.When stored in reaction vessel recovering mechanism it is full or
After nonresident portion reaction vessel box, it can be taken out from the lower section of reaction vessel recovering mechanism, can be realized reaction vessel box in this way
Continuous recycling, is easy to use.Reaction vessel recovering mechanism includes time recycling transmission structure and being set on recycling transmission structure
It admits a child into a nursery school plate, recycling supporting plate is able to drive recycling supporting plate and moves up and down for carrying reaction vessel box, recycling transmission structure, in turn
Realize the recycling and storage of reaction vessel box.Preferably, recycling transmission structure is using energy such as gear & rack structure, synchronous band structures
It enough realizes the structure of elevating movement, and power source is used as using motor etc..
Again optionally, reaction vessel automatic conveying device 17 further includes reaction vessel positioning blanking mechanism, and reaction vessel is fixed
Position blanking mechanism is set to the top of reaction vessel recovering mechanism.Reaction vessel box on reaction vessel hoisting mechanism can be pushed away
Mechanism is sent to push on reaction vessel positioning blanking mechanism.It grabs cup device 16 and grabs reaction on reaction vessel positioning blanking mechanism
Reaction vessel in container, and be transferred in reaction unit 13 and carry out the operations such as sample-adding sheet, reagent.Reaction after the completion of use
Container positions blanking mechanism by reaction vessel and falls in reaction vessel recovering mechanism.It is understood that reaction vessel is fixed
Position blanking mechanism can facilitate the positioning of reaction vessel box conveniently to grab cup so that the position of reaction vessel box is fixed always simultaneously
Device 16 takes out the reaction vessel in reaction vessel box at reaction vessel blanking mechanism.Reaction vessel in reaction vessel box is complete
After portion is removed, reaction vessel box will be recovered, and be fallen at this point, reaction vessel box can position blanking mechanism by reaction vessel
And be stored in reaction vessel recovering mechanism, facilitate the recycling of reaction vessel box.Specifically, reaction vessel positioning blanking mechanism is logical
The channel that falls that slide switch door turns on or off reaction vessel box is crossed, when opening falls channel, reaction vessel box is fallen to
In reaction vessel recovering mechanism；When closing falls channel, reaction vessel box can be located in reaction vessel positioning blanking mechanism；And
And reaction vessel positioning blanking mechanism has positioning element, delivery device is by reaction vessel box from reaction vessel hoisting mechanism
When top layer is pushed on reaction vessel positioning blanking mechanism, reaction vessel box can be abutted with positioning element, guarantee reaction vessel
The crawl of cup device 16 is conveniently grabbed in box accurate positioning.
Optionally, reaction vessel recovering mechanism includes the first reaction vessel recovering mechanism and recycles positioned at the first reaction vessel
The second reaction vessel recovering mechanism below mechanism, the second reaction vessel recovering mechanism can be with the first reaction vessel recovering mechanisms pair
It connects and separates.Reaction vessel automatic conveying device 17 further includes drawer mechanism, reaction vessel storing mechanism and the second reaction vessel
Recovering mechanism is set in drawer mechanism, and drawer mechanism is able to drive reaction vessel storing mechanism and the second reaction vessel recycling machine
Structure is extracted out and is pushed into relative to chemiluminescence detector.When drawer mechanism is extracted out, reaction vessel storing mechanism is mentioned with reaction vessel
Organizational separation is risen, the second reaction vessel recovering mechanism is separated with the first reaction vessel recovering mechanism, at this time can be to reaction vessel
Reaction vessel box is loaded in storing mechanism, can take out the reaction vessel box in the second reaction vessel recovering mechanism after use.It takes out
When drawer mechanism is pushed into, reaction vessel storing mechanism is docked with reaction vessel hoisting mechanism, the second reaction vessel recovering mechanism and the
The docking of one reaction vessel recovering mechanism, reaction vessel storing mechanism can transmit reaction vessel box to reaction vessel hoisting mechanism,
Second reaction vessel recovering mechanism can continue to recycling reaction vessel box.Specifically, drawer mechanism can be slided along sliding rail, and can
The extraction and push-in of drawer mechanism are automatically controlled by chemiluminescence detector；Manual type can also be used by the pumping of drawer mechanism
Out with push-in.
Moreover, reaction vessel automatic conveying device 17 is located at the side of reaction unit 13, grabs the crawl reaction of cup device 16 and hold
The reaction vessel of sky in device automatic conveying device 17 is simultaneously transferred in reaction unit 13, so that reaction unit 13 carries out subsequent behaviour
Make.The path for grabbing 16 transfer reaction container of cup device can be reduced in this way, improve the speed of reaction vessel transfer, and then raisingization
Learn the operational efficiency of luminometer.Further, also there is chemiluminescence detector new cup to grab cup mechanism 161, that is, grab cup
Setting 16 includes that new cup grabs cup mechanism 161.New cup grabs the corresponding reaction vessel automatic conveying device 17 of cup mechanism 161 and reaction unit 13
Setting, for the reaction vessel in reaction vessel automatic conveying device 17 to be transferred in reaction unit 13.It is understood that
New cup, which grabs cup mechanism 161, to position the reaction vessel grabbed in reaction vessel box on blanking mechanism in reaction vessel, and shifts
Into reaction unit 13, to carry out the operations such as sample-adding sheet, reagent adding.
As an embodiment, cleaning device 14 is used to remove the impurity in reaction vessel, to guarantee measuring device
The accuracy of testing result when 15 detection determinand.Specifically, cleaning device 14 includes cleaning turntable, cleaning needle assemblies and absorption
Part.On the evenly spaced periphery for being carried on cleaning turntable of multiple reaction vessels.The revolution rail of reaction vessel is arranged in adsorption piece
The side of mark.Cleaning turntable rotates and reaction vessel is driven to do revolution motion around the central axis of cleaning turntable, in adsorption piece
Under effect, determinand can be attracted on the side wall of reaction vessel.When cleaning needle assemblies are cleaned, cleaning needle assemblies can
To inject cleaning solution, the mixture in reaction vessel is cleaned by cleaning solution, after the completion of cleaning, cleaning needle assemblies can
By the waste liquid discharge in reaction vessel.
It is understood that the quantity of cleaning needle assemblies can be one or more groups of, with to the impurity in reaction vessel into
The primary cleaning of row cleans twice, cleans even more be cleaned multiple times three times, is cleaned at least once to the impurity in reaction vessel
Afterwards, finally waste liquid is discharged by cleaning needle assemblies, leaves determinand and measures or carry out secondary reagent adding operation.Also,
Adsorption piece adsorbs determinand using such as magnet magnetic part, cleans needle assemblies and cleaning turntable uses motor as power source,
And using the transmitting of the realizations campaign such as gear transmission structure and/or toothed belt transmission structure.
Moreover, cleaning device 14 is located at the side of reaction unit 13.Reaction vessel is in reaction unit 13 and cleaning device 14
Between when shifting, the path of reaction vessel transfer can be reduced.Also there is chemiluminescence detector cleaning to grab cup mechanism 164, i.e.,
Grabbing cup device 16 further includes that cup mechanism 164 is grabbed in cleaning, and the corresponding cleaning device 14 of cup mechanism 164 is grabbed in cleaning and reaction unit 13 is set
It sets, for shifting reaction vessel between reaction unit 13 and cleaning device 14.That is, 164 energy of cup mechanism is grabbed in cleaning
Enough reaction vessels by after the completion of incubating in reaction unit 13 are transferred on the cleaning turntable of cleaning device 14；Cup mechanism is grabbed in cleaning
164 can also will need the reaction vessel for adding reagent to be again transferred in reaction unit 13 after the completion of cleaning.
As an embodiment, measuring device 15 is for detecting the determinand in reaction vessel, to obtain
The corresponding detection parameters of sample.Specifically, measuring device 15 includes bottom plate, inner casing, shell, upper cover, measurement turntable, driving machine
Structure, the first substrate spray head, the second substrate spray head, photomultiplier tube detection components and waste suction liquid needle assemblies.Bottom plate, shell and upper cover
The accommodating space of measuring device 15 is surrounded, measurement turntable, inner casing are located in accommodating space, bottom plate, shell, inner casing, upper cover and survey
It measures turntable and constitutes a darkroom.Measuring device 15 also have multiple reactor vessel processes stations, multiple reactor vessel processes stations it
Between mutually every light seal.In the present invention, it is illustrated so that the quantity of reactor vessel processes station is four as an example.Four reactions
Container is handled and is mutually sealed every light between station.When measurement:
First reactor vessel processes station (reaction vessel enters station): reaction vessel is in the first reactor vessel processes station
Place is placed into or is taken out in measuring device 15；
(excitation I station of substrate is added) in second reactor vessel processes station: the first substrate spray head is set to the second reaction appearance
Device is handled at station, excitation substrate I is added to reaction vessel；
Third reactor vessel processes station (reaction vessel measurement station): the second substrate spray head is set to third reaction vessel
It handles at station, excitation substrate II is added to reaction vessel, photomultiplier tube detection components are set at third reaction vessel
At science and engineering position；
4th reactor vessel processes station (takes out waste liquid station): waste suction liquid needle assemblies are set to the 4th reactor vessel processes work
With the waste liquid of abstraction reaction container at position.
First reactor vessel processes station, the second reactor vessel processes station, third reactor vessel processes station and the 4th
Reactor vessel processes station is arranged successively and mutually seals every light, the first reactor vessel processes station along measurement turntable sense of rotation
Diagonally it is arranged with third reactor vessel processes station, the second reactor vessel processes station and the 4th reactor vessel processes station are diagonal
Setting.Bottom plate, inner casing, shell, upper cover and the structure setting and the function of each reactor vessel processes station processing that measure turntable
And its used mechanism is adapted.Driving mechanism is for driving measurement turntable rotation, and driving mechanism is using motor etc. as dynamic
Power source, and using the transmitting of the realization power such as gear drive motivation structure, with driving measurement turntable rotation.
Optionally, measuring device 15 further include every optical assembly, by separating each reactor vessel processes station every optical assembly, with
Guarantee every optical property.Further, it is realized every the structure of light every light processing every optical assembly using light insulation pad or other realizations.
Moreover, measuring device 15 is located at the side of reaction unit 13, and it is disposed adjacent with cleaning device 14.Different samples
There are difference, some samples directly to be measured after cleaning by measuring device 15 for the process of processing, and some samples need
It is then transferred to after cleaning in reaction unit 13 and carries out the operation such as secondary reagent adding, some samples are directly measured without cleaning.
Therefore, measuring device 15 and cleaning device 14 are located at the side of reaction unit 13, and measuring device 15 is adjacent with cleaning device 14
Setting, when such reaction vessel shifts between measuring device 15, cleaning device 14 and reaction unit 13, can shorten reaction
The speed of reaction vessel transfer is improved, and then improves the operational efficiency of chemiluminescence detector in the path of container transfer.Chemistry hair
Also there is optical detector measurement to grab cup mechanism 165, that is, grabbing cup device 16 further includes that cup mechanism 165 is grabbed in measurement, and cup mechanism is grabbed in measurement
165 corresponding cleaning devices 14 and measuring device 15 are arranged, for the reaction vessel after cleaning in cleaning device 14 to be transferred to survey
It measures in device 15.That is, cup mechanism 165 is grabbed in measurement can will clean on turntable to the reaction vessel transfer after impurity cleaning
Each reactor vessel processes work is successively turned over onto the measurement turntable of measuring device 15, and by measuring driven by rotary disc reaction vessel
Position, to detect to the determinand in reaction vessel, obtains the detection parameters of sample.
As an embodiment, chemiluminescence detector further includes evenly mixing device, for adding in reaction unit 13
The reaction vessel for adding sample and reagent carries out mixing operation, so that mixture is sufficiently mixed, then carries out incubation operation again.It is mixed
Even device is located at the side of reaction unit 13, can facilitate evenly mixing device to mixed in the reaction vessel in reaction unit 13 in this way
It closes object and carries out mixing operation.Specifically, evenly mixing device includes elevating movement mechanism and mixing mechanism, mixing mechanism is set to lifting
On movement mechanism.Elevating movement mechanism can be such that mixing mechanism moves up and down, and enable mixing mechanism and reaction vessel height
The seamed edge in degree direction is in contact, and subsequent mixing mechanism, which is able to drive reaction vessel movement, rocks reaction vessel at high speed, protects
Mixture is demonstrate,proved to be uniformly mixed.It is understood that elevating movement mechanism is to be able to carry out the rail structure of elevating movement and/or same
Band structure etc. is walked, mixing mechanism is the structure that can be realized eccentric rotary, also, elevating movement mechanism and mixing mechanism use
Motor is as power source.Certainly, in other embodiments of the invention, the form of stirring rod is can also be used to anti-in evenly mixing device
It answers the mixture in container to stir, realizes that mixture is uniformly mixed.
As an embodiment, reaction unit 13 includes buffering disc mechanism 131 and independently of buffering disc mechanism 131
Disc mechanism is reacted, buffering disc mechanism 131, which accommodates reaction vessel and executes, adds sample operations, and reaction disc mechanism accommodates reaction vessel simultaneously
It executes reagent adding, mixing and incubates operation.Empty reaction vessel is transferred on buffering disc mechanism 131, subsequent sample charging mechanism
121 can be transferred to sample in the reaction vessel in buffering 131 overhead of disc mechanism.The reaction vessel for adding sample can be transferred
Onto reaction disc mechanism, by the first pipetting device and the second pipetting device respectively by the first reagent storing mechanism 111 and second
Then agent transfer in reagent storing mechanism 112 again executes reaction disc mechanism mixed into the reaction vessel of reaction disc mechanism
Even and incubation operation.Moreover, also there is chemiluminescence detector sample to grab cup mechanism 162, that is, cup device 16 is grabbed with sample
Cup mechanism 162 is grabbed, sample grabs the corresponding buffering disc mechanism 131 of cup mechanism 162 and reaction disc mechanism setting, for that will buffer disc mechanism
Reaction vessel on 131 is transferred in reaction disc mechanism.Sample charging mechanism 121 adds the samples to the reaction of buffering disc mechanism 131
After in container, sample is grabbed cup mechanism 162 and can be transferred to the reaction vessel for adding sample in reaction disc mechanism, and in reaction tray
Reagent adding, mixing are continued to execute in mechanism and is incubated operates.
Further, reaction disc mechanism include in independently operated reaction disc mechanism 133 with react outer disc mechanism 132, instead
Outer disc mechanism 132 is answered to be sheathed on the outside for reacting interior disc mechanism 133；It reacts outer disc mechanism 132 and accommodates reaction vessel and execute and add
Reagent and mixing operate, and disc mechanism 133, which accommodates reaction vessel and executes, in reaction incubates operation.It is added on buffering disc mechanism 131
The reaction vessel of sample is transferred on the outer disc mechanism 132 of reaction, and then, the first pipetting device and the second pipetting device can incite somebody to action
Agent transfer then reacts appearance to add sample and reagent to reacting in the reaction vessel for adding sample on outer disc mechanism 132
Device is mixed, so that mixture is uniformly mixed.Reaction vessel after mixing is transferred in reaction in disc mechanism 133, and
Mixture in reaction vessel is incubated, so that sample reaches optimal reaction condition, facilitates chemiluminescence detector
Luminescence detection apparatus detects sample parameter.It should be noted that the outer disc mechanism 132 of reaction of the invention is in other embodiments
The reaction vessel that empty reaction vessel or carrying have the solution such as calibration liquid can also be carried.In the present embodiment, only with reaction
Outer disc mechanism 132 is illustrated for carrying the reaction vessel with sample.
Further, the setting coaxial with interior disc mechanism 133 is reacted of outer disc mechanism 132 is reacted, reaction tray can be reduced in this way
The volume of mechanism, and then reduce machine volume.Certainly, reacting outer disc mechanism 132 in other embodiments of the invention can also
It is arranged independently of disc mechanism 133 in reacting.Optionally, buffering disc mechanism 131 is arranged independently of outer disc mechanism 132 is reacted, can also
It is arranged layer by layer with reacting interior disc mechanism 133, reacting outer disc mechanism 132.Chemiluminescence detector also there is incubation to grab cup mechanism
163, that is, grabbing cup device 16 further includes incubating to grab cup mechanism 163, incubation grab cup mechanism 163 it is corresponding react outer disc mechanism 132 with instead
Interior disc mechanism 133 is answered to be arranged, for shifting reaction vessel reacting outer disc mechanism 132 with reacting between interior disc mechanism 133.
Specifically, buffering disc mechanism 131 includes that the buffering carrier of carrying reaction vessel and driving buffering carrier turn
Dynamic buffered-display driver structure, buffered-display driver structure is used as power source using motor etc., and is realized by toothed belt transmission structure etc.
The transmitting of movement, driving buffering carrier rotation.React the reaction external disk carrier that outer disc mechanism 132 includes carrying reaction vessel
And the reaction external disk driving structure of drive response external disk carrier rotation, reaction external disk driving structure is using motor etc. as dynamic
Power source, and pass through the transmitting of the realizations campaigns such as toothed belt transmission structure, the rotation of drive response external disk carrier.Disc mechanism in reacting
133 include dish driving knot in the reaction inner disc carrier of carrying reaction vessel and the reaction of drive response inner disc carrier rotation
Structure, reaction inner disc driving structure is used as power source using motor etc., and passes through the transmitting of the realizations campaigns such as toothed belt transmission structure,
The rotation of drive response inner disc carrier.Moreover, disc mechanism 133 also has heating structure and insulation construction in reacting, in reaction
Reaction vessel in disk carrier is heated, and provides incubation environment for the reaction of mixture in reaction vessel, guarantee sample with
Reagent reaction can be normally carried out, and then facilitate it is subsequent to sample carry out shine detection.
Still further, the outer disc mechanism 132 of reaction has multiple reaction stations, including add a glass station, the first reagent adding work
Position, the second reagent adding station, mixing station and incubation take a glass station.It should be noted that plus cup station, reagent adding station,
Mixing station and incubating takes glass station around reaction external disk carrier setting, and the fixation position of chemiluminescence detector adds cup
Station, reagent adding station, mixing station and incubation take glass station not become with the rotation of reaction external disk carrier
Change.Reaction external disk driving structure can drive response external disk carrier drive reaction vessel to turn to corresponding reaction station, and
Corresponding operation is executed in reaction station.
Add a glass station correspond to sample grab cup mechanism 162 setting, sample grab cup mechanism 162 can will buffer carrier in it is anti-
Answer container that a glass station is being added to be placed into reaction external disk carrier.First reagent adding station corresponds to the first reagent storing mechanism 111
Setting, corresponding second reagent storing mechanism 112 setting of the second reagent adding station, the first pipetting device is in the first reagent storing mechanism
Reagent is drawn in 111, and is added reagent in reaction vessel at the first reagent adding station, and the second pipetting device is in the second examination
Reagent is drawn in agent storing mechanism 112, and is added reagent in reaction vessel at the second reagent adding station.Mix station pair
Evenly mixing device is answered to be arranged, evenly mixing device can mix the mixture in the reaction vessel for reacting external disk carrier in mixing station
It is even.Incubation takes corresponding incubate of glass station to grab the setting of cup mechanism 163, and incubation, which grabs cup mechanism 163, to take a glass station crawl in incubation
The reaction vessel in external disk carrier is reacted, and is transferred in reaction inner disc carrier.
It is understood that reaction external disk carrier drives reaction vessel thereon to rotate corresponding station, so that reaction
Container is performed corresponding operation in corresponding station, and such as reagent adding mixes and cup is taken to operate；When reaction external disk carrier rotation
To when adding glass station, sample grabs cup mechanism 162 and is transferred to reaction vessel in reaction external disk carrier from buffering carrier.More
Further, a glass station, the first reagent adding station, the second reagent adding station, mixing station and incubation is added to take glass station opposite
It arranges in reaction external disk carrier sequence.Illustrate the operation for reacting external disk carrier: sample with the running track of a reaction vessel
Originally it grabs cup mechanism 162 and is placed into the reaction vessel for adding sample in reaction external disk carrier adding at glass station；Then, it reacts
External disk carrier drives reaction vessel to be moved at the first reagent adding station and the second reagent adding station from glass station from adding, and first
Pipetting device and the second pipetting device add reagent on reaction vessel at the first reagent adding station and the second reagent adding station
In；After the completion of adding reagent, reaction external disk carrier drives reaction vessel to move to mixing station from the second reagent adding station
Mixture in reaction vessel is uniformly mixed by place, evenly mixing device at mixing station；After the completion of mixing, external disk carrier is reacted
It drives reaction vessel to move to incubation from mixing station to take from glass station, incubation grabs cup mechanism 163 and takes general at glass station in incubation
Reaction vessel takes out, and is transferred in reaction inner disc carrier；Then, reaction external disk carrier, which returns, adds cup station, so
It moves in circles, realizes continuous operation.
It should be noted that chemiluminescence detector in actual moving process, reacts outer 132 continuous operation of disc mechanism,
External disk carrier is reacted after reaction external disk carrier operation a cycle and turns to adjacent station from a station, is adding cup
While station adds reaction vessel to reaction external disk carrier, pipetting device is shifted in reagent adding station into reaction vessel
Reagent, the mixture of reaction vessel is uniformly mixed by evenly mixing device in mixing station, while incubation is grabbed cup mechanism 163 and taken in incubation
Reaction vessel is transferred in reaction inner disc carrier by cup station.That is, after reaction external disk carrier rotation a cycle,
It is performed simultaneously in corresponding station and adds cup, reagent adding, mixes and cup is taken to operate.
Optionally, reaction station further includes that a glass station is put in cleaning, and cleaning puts that glass station is located at plus cup station is added an examination of with first
Between agent station.Sample after the completion of reaction inner disc carrier incubates can be cleaned grabbing cup mechanism 164 and be transferred to cleaning device 14
Middle carry out cleaning operation, to wash impurity in reaction vessel, since part sample also needs secondary addition reagent to be reacted,
At this point, cup mechanism 164 is grabbed in cleaning to put a glass station transfer in cleaning for the reaction vessel after the completion of cleaning in cleaning device 14
Into reaction external disk carrier.Also, sample after reacting incubation in inner disc carrier needs directly to carry out secondary or even more
When secondary reagent adding operates, that is, the reaction vessel after incubating just carries out secondary or multiple reagent adding without the cleaning of cleaning device 14 and grasps
Make, at this point, cleaning grab cup mechanism 164 can also will react inner disc carrier in incubate after reaction vessel cleaning put glass station turn
It moves on in reaction external disk carrier, the subsequent process of subsequent process and the operation of a reagent adding is identical, herein not one by one
It repeats.
Optionally, reaction external disk carrier also has buffer station relative to chemiluminescence detector, and buffer station is located at
Between two reaction stations of arbitrary neighborhood, buffer station can make two adjacent reaction stations and adjacent reaction station with
Spacing between buffer station is equal.It is understood that buffer station is waiting station, reaction external disk carrier moves to slow
It rushes at station, any operation is not carried out, the operating time can be contributed in this way for pending corresponding operating.
In the present embodiment, buffer station includes the first buffer station, the second buffer station and third buffer station, and adds cup
Station, the first buffer station, cleaning are put a glass station, the second buffer station, the first reagent adding station, the second reagent adding station, are mixed
Even station, incubation take glass station and third buffer station in the direction of the clock between circumferential direction of reaction external disk carrier etc.
Away from being arranged.Reaction external disk driving structure drive response external disk carrier moves to above-mentioned reaction station and buffering work respectively
Position, and execute corresponding operation.It should be noted that the every drive response external disk carrier rotation one of reaction external disk driving structure
Secondary, reaction external disk carrier enables the reaction vessel on reaction external disk carrier to respectively correspond around 1/9 circle of its center of circle rotation
Nine station settings, and corresponding operation is executed by the device of corresponding station.Each station is using above-mentioned setting and cooperates corresponding
Operation structure after, can effectively improve reaction external disk carrier working efficiency so that reaction external disk carrier and other
Functional module such as buffer carrier, the first pipetting device, the second pipetting device, reaction inner disc carrier between process flow more
Be it is smooth, improve the operational efficiency of chemiluminescence detector.
First reagent storing mechanism 111 is arranged side by side with the second reagent storing mechanism 112 in the week for reacting outer disc mechanism 132
Side, and corresponding first reagent adding station and the second reagent adding station are arranged.Certainly, when react in disc mechanism 133 with react external disk
When mechanism 132 is integrated, as reaction disc mechanism, the first reagent storing mechanism 111 are set side by side with the second reagent storing mechanism 112
It is placed in the side of reaction disc mechanism, to shorten the path of agent transfer.It should be noted that new, cup grabs cup mechanism 161, sample is grabbed
Cup mechanism 162, incubation grab cup mechanism 163, cup mechanism 164 is grabbed in cleaning, cup mechanism 165 is grabbed in measurement can be using grabbing a glass driving group
Part such as grabs glass control assembly and grabs glass arm component at the crawl and transfer for realizing reaction vessel.It is understood that grabbing cup control group
Part can use general control system such as controller etc., and grabbing glass driving assembly can be driven using driving motor mate gear
The motion control of glass arm component is grabbed in the realizations such as component, V belt translation component or chain kit, so that grabbing glass arm component carries out water
The flat movement with vertical direction, guarantee grab glass arm component movement in place and grab transfer reaction container.Moreover, grabbing glass arm component
End has handgrip, grabs reaction vessel by handgrip.
As an embodiment, chemiluminescence detector further includes sample conveying device 19, for conveying sample
To sample position；Sample position is located at the side of buffering disc mechanism 131 and corresponding sample charging mechanism 121 is arranged；Sample charging mechanism 121 exists
Sample position is drawn sample and is transferred in the reaction vessel of buffering disc mechanism 131.Sample conveying device 19 is to be measured for conveying
Sample realizes the Automatic Conveying of sample to be tested, to improve sample introduction efficiency.Moreover, sample is transported to by sample conveying device 19
Sample position, sample charging mechanism 121 are drawn sample at sample position and are transferred in the reaction vessel of buffering disc mechanism 131, this
Sample can shorten the distance of sample transfer, improve sample transfer efficiency.
Specifically, sample conveying device 19 includes sample storing mechanism 191 and is detachably connected with sample storing mechanism 191
Sample conveying mechanism 192, store multiple sample racks for being mounted with sample, sample storing mechanism 191 in sample storing mechanism 191
Sample rack is transported in sample conveying mechanism 192, and sample position is delivered to by sample conveying mechanism 192.Sample conveying dress
Setting 19 further includes sample transfer device, and sample transfer device can make the sample rack in sample storing mechanism 191 be transferred to sample
In conveying mechanism 192.Sample storing mechanism 191 has conveying mouth, and sample rack is transferred to sample transfer device by conveying mouth
On, and sample rack is transferred on sample conveying mechanism 192 by sample transfer device.Sample conveying mechanism 192 includes track
Component and become rail structure, sample transfer device can dock with the transmission channel of rail assembly, so that rail assembly is by sample
It is transported to sample position.Rail assembly realizes the conveying of sample rack using synchronous band structure etc..Become rail structure and enables to sample
Frame shifts between multiple transmission channels of rail assembly.Optionally, sample transfer device includes ferry-boat trolley, small by ferrying
Vehicle realizes that sample storing mechanism 191 is connected to transmission channel.Certainly, sample transfer device can be also transfer orbit or crawl knot
Structure etc. can be realized the structure of sample rack transfer.Moreover, sample conveying device 19 can also in other embodiments of the invention
The conveying of sample is realized by the way of sample disk.
When 19 transmission sample of sample conveying device, the sample rack equipped with sample to be tested is put sample manually by operator
In storing mechanism 191；Sample storing mechanism 191 is mounted on chemiluminescence detection by connection structures such as supporting element and suspenders
On the apparatus subject of instrument.Ferry-boat trolley chooses sample to be tested frame and delivers sample rack to the track groups of sample conveying mechanism 192
In the transmission channel of part, sample rack is transported to by sample position by rail assembly.After the completion of the sampling of sample charging mechanism 121, become
Rail structure changes the transmission channel for the sample rack that sampling finishes, and the sample rack that sampling finishes is transferred to sample structure for conveying
Input terminal, and the sample rack that sampling finishes is transported in sample storing mechanism 191 by ferry-boat trolley, operator recycles sample
The sample rack finished is sampled in this storing mechanism 191.
When more chemiluminescence detector splicings are in use, the input terminal of sample conveying mechanism 192 can be stored with sample
Mechanism 191 is docked, or is docked with the output end of the sample conveying mechanism 192 of another chemiluminescence detector.Sample conveyer
The output end of structure 192 can be docked with the input terminal of the sample conveying mechanism 192 of another chemiluminescence detector.That is,
Sample storing mechanism 191 by a chemiluminescence detector is the sample conveying mechanism 192 of more chemiluminescence detectors
Transmission sample realizes pile line operation.It is illustrated so that three chemiluminescence detectors splice as an example.First chemiluminescence inspection
The input terminal for surveying the sample conveying mechanism 192 of instrument is docked with sample storing mechanism 191, the sample of First chemiluminescence detector
The output end of conveying mechanism 192 is docked with the input terminal of second sample conveying mechanism 192, second sample conveying mechanism 192
Output end docked with the input terminal of third platform sample conveying mechanism 192.It is deposited by the sample of First chemiluminescence detector
Storing up mechanism 191 is that three chemiluminescence detectors convey sample.
As an embodiment, sample charging mechanism 121 includes straight line slide unit, sample-adding module and hinged cantilever, is loaded mould
Block is used to be loaded.Fluid suction head can be generally set in sample-adding module, to be sampled.In the present invention, sample-adding module is ADP mould
Block.ADP module: i.e. air-displacement pipetting module, Chinese name are as follows: pneumatic liquid relief module.In other realities
It applies in example, sample-adding module may be the sampling module being mainly made of steel needle, fluid path and plunger pump.Sample-adding module is mounted on rotation
Turn on cantilever, sample-adding module is supported by hinged cantilever, while sample-adding module being driven to turn around the center of rotation of hinged cantilever
It is dynamic.Hinged cantilever is slidably mounted on straight line slide unit, drives hinged cantilever to move linearly by straight line slide unit.Specifically, straight
Line slide unit is sliding-rail sliding structure and/or synchronous band structure etc., to drive hinged cantilever to move in a straight line.In this way, straight line slide unit
Cooperate with hinged cantilever so that being loaded module after sample position draws sample, and the reaction for being transferred to buffering disc mechanism 131 is held
In device 20.
Since the fluid suction head in sample-adding module is medical disposable material, to guarantee to shift the continuity of sample, need to pass automatically
Defeated fluid suction head.Therefore, chemiluminescence detector further includes consumption material box loading device 18, for transmitting the consumption for loading fluid suction head automatically
Material the storage box, and consumptive material the storage box can also be delivered to " loaded " position by consumption material box loading device 18；Sample charging mechanism 121 is loading position
Loading fluid suction head is set, and sample is shifted by fluid suction head.Fluid suction head is filled in consumptive material the storage box.Consumption material box loading device 18 includes
Consumption material box accommodates storehouse, consumption material box surge bunker, consumption material box transport mechanism and consumption material box gripping body.Consumption material box transport mechanism is using same
It walks band structure etc. and realizes the horizontal movement with vertical direction, so that consumptive material the storage box can be transferred to " loaded " position.Consumption material box
Consumptive material the storage box can be accommodated in consumption material box and be passed between storehouse, consumption material box surge bunker and consumption material box transport mechanism by gripping body
It send.In the present embodiment, fluid suction head is TIP head, the TIP disposable tips for sampling.
Operator accommodates in consumption material box be placed with the consumptive material the storage box for filling fluid suction head in storehouse manually, on consumption material box surge bunker
Two buffering frames it is vacant.The consumptive material the storage box that consumption material box gripping body clamps in a consumption material box receiving storehouse is placed on consumption material box
On the delivery frame of transport mechanism, delivery frame transports consumptive material the storage box to " loaded " position, " loaded " position can to consumptive material the storage box into
Luggage carries positioning, so that the sample-adding module of sample charging mechanism 121 carries out fluid suction head loading in " loaded " position.Consumption material box gripping body folder
After taking a consumptive material the storage box to place on delivery frame, then a consumptive material the storage box is grabbed from consumption material box receiving storehouse and is placed on consumptive material
In first buffering frame of box surge bunker.After being finished to the fluid suction head in the consumptive material the storage box of " loaded " position, delivery frame is transported
Empty consumptive material the storage box returns, and the empty consumptive material the storage box of consumption material box gripping body clamping is placed on second on consumption material box surge bunker
In a buffering frame.After empty consumptive material the storage box is placed in second buffering frame on consumption material box surge bunker, consumption material box catching device
The consumptive material the storage box that structure clamps in first buffering frame is placed on delivery frame, and delivery frame transports consumptive material the storage box.Consumption material box folder
It takes mechanism to clamp the empty consumptive material the storage box in second buffering frame to be placed on consumption material box receiving storehouse.Consumption material box gripping body folder
One new consumptive material the storage box for filling fluid suction head of clamping from consumption material box receiving storehouse is taken to be placed on the first of consumption material box surge bunker
In a buffering frame.The above movement is repeated, realizes the continuous loading of consumptive material the storage box.It is understood that when empty consumptive material storage
When box fills entire consumption material box receiving storehouse, consumption material box receiving storehouse can be directly extracted out, user takes empty consumptive material the storage box away, and
Consumption material box, which accommodates, loads new consumptive material the storage box in storehouse.After the completion of loading, then consumption material box is accommodated into storehouse and is pushed into, consumption material box catching device
Structure clamps consumptive material the storage box again, and repeats aforesaid operations.Since two buffering frames are arranged, after extraction consumption material box accommodates storehouse,
Instrument internal is also able to achieve loading and the recycling of consumptive material the storage box, to realize continuous load.
Further, consumption material box loading device 18 is located at the separate buffering disc mechanism of reaction vessel automatic conveying device 17
131 side, and corresponding sample charging mechanism 121 is arranged.The transmission path of consumptive material the storage box can be shortened in this way, improve transmission effect
Rate；It can also the space occupied is reduced, to reduce the machine volume of chemiluminescence detector.
As an embodiment, chemiluminescence detector further includes pedestal 10, and pedestal 10 has carrying platform, reaction
The carrying that device 13, measuring device 15, cleaning device 14, dispenser 12 and reagent storage means 11 are all set in pedestal 10 is flat
On platform.Reagent storage means 11, consumption material box loading device 18, sample conveying device 19 sample conveying mechanism 192 and sample deposit
Storage mechanism 191 encloses the surrounding set on carrying platform respectively, and is located at the marginal position of carrying platform.Reaction vessel transmits dress automatically
17 are set between consumption material box loading device 18 and the buffering disc mechanism 131 of reaction unit 12.As shown in Figure 1, reagent storage dress
It sets 11 the first reagent storing mechanism 111 and the second reagent storing mechanism 112 is located at marginal position on front side of carrying platform, consumptive material
Box loading device 18 is located at the marginal position on the left of carrying platform, and sample storing mechanism 191 is located at the edge on the right side of carrying platform
Position, sample conveying mechanism 192 are located at the marginal position on rear side of carrying platform.Reaction disc mechanism is located at the middle region of carrying platform
Domain, buffering disc mechanism 131, reaction vessel automatic conveying device 17, measuring device 15 and cleaning device 14 are enclosed set on reaction tray machine
The side of structure；Sample charging mechanism 121 is located between buffering disc mechanism 131 and sample conveying mechanism 192；Evenly mixing device is set to reaction
On outer disc mechanism 132, and the corresponding station that mixes is arranged.Since the primary operational face of sample storing mechanism 191 is in right side, operator
For member from right side to 191 sample loading frame of sample storing mechanism, trolley of ferrying takes out sample rack from sample storing mechanism 191, and
It is sent into the sample conveying mechanism 192 on rear side of instrument, sample rack is transported to sample position, sample is drawn by sample charging mechanism 121.
After the completion of absorption, sample rack is sent back in sample storing mechanism 191 by sample conveying mechanism 192 through ferry-boat trolley again, by operator
Member takes out.
Further, chemiluminescence detector further includes control device and fluid path device；Control device and reaction unit 13,
Measuring device 15, cleaning device 14, dispenser 12 and reagent storage means 11 are electrically connected, and fluid path device and dispenser 12 connect
It connects；Pedestal 10 also has accommodating space, and accommodating space is located at the lower section of carrying platform, and control device and fluid path device are set to appearance
In between emptying.Control loads the automatic control for being used to realize each components of chemiluminescence detector, so that each zero
Part can be realized automatic operation, improve working efficiency.The main cleaning machine of fluid path device and pipetting device 122 and cleaning device 14
Structure is connected to secondary wiper mechanism, and for pipetting device 122 and main wiper mechanism and secondary wiper mechanism provides cleaning solution or discharge is useless
Liquid.
Moreover, 17 top quartile of reaction vessel automatic conveying device, in the top of carrying platform, lower part quartile is flat in carrying
In lower section, that is, accommodating space of platform.Specifically, drawer mechanism, reaction vessel storing mechanism and the second reaction vessel recovering mechanism
It is set in accommodating space, reaction vessel hoisting mechanism and the first reaction vessel recovering mechanism are located above carrying platform.Work as pumping
When drawer mechanism is extracted out, reaction vessel box is being loaded to reaction vessel storing mechanism and is being returned in the second reaction vessel recovering mechanism
While receiving reaction vessel box, reaction vessel hoisting mechanism and the first reaction vessel recovering mechanism still are able to realize reaction vessel box
Load and recycling, realize reaction vessel box it is continuous load with continuously recycle, instrument is operated normally.Carrying platform
Reaction vessel hoisting mechanism and the first reaction vessel recovering mechanism can be supported, lock out operation when drawer mechanism being facilitated to extract out；
Accommodating space can also accommodate drawer mechanism, reaction vessel storing mechanism and the second reaction vessel recovering mechanism, so that space is closed
Reason utilizes, to guarantee machine volume.
The present invention also provides a kind of detection method of chemiluminescence detector, the detection method based on the above embodiment in
Chemiluminescence detector.Detection method includes the following steps:
It is loaded this step；Sample is transferred in reaction vessel；
Reagent adding step；By agent transfer into reaction vessel；
Mix step；Mixture in reaction vessel is uniformly mixed；
Incubation step；Incubation operation is carried out to the mixture being uniformly mixed in reaction vessel；
Separation cleaning step；The impurity in reaction vessel after removal incubation；
Measuring process；Determinand in reaction vessel after cleaning is detected.
For chemiluminescence detector of the invention when to pattern detection, reaction vessel automatic conveying device 17 is by reaction vessel
Box is transferred in reaction vessel positioning blanking mechanism, and consumptive material the storage box is transported to " loaded " position by consumption material box loading device 18.Sample
Sample rack is transferred to sample position by sample conveying mechanism 192 in sample storing mechanism 191 by this conveying device 19.By new
Cup is grabbed cup mechanism 161 and the reaction vessel in reaction vessel box is transferred in buffering disc mechanism 131 in case using.Sample charging mechanism
121 sample-adding module loads a new TIP head, and draws one that sample is transferred to buffering disc mechanism 131 in sample position
Or in multiple reaction vessels.Sample grabs cup mechanism 162 and the reaction vessel buffered in disc mechanism 131 is transferred to reaction external disk machine
In structure 132；Reaction external disk carrier drives reaction vessel to move to reagent adding station, and pipetting device 122 draws reagent and stores machine
Reagent in structure is simultaneously transferred in reaction vessel in reagent adding station；Reaction external disk carrier drives reaction vessel to move to mixing
Station, evenly mixing device drives reaction vessel to vibrate with fixed frequency, to mix the mixture in reaction vessel.Reaction after mixing
Container is carried out incubation in disc mechanism 133 in reaction and is operated by incubating to grab cup mechanism 163 and be transferred to, so that sample is sufficiently anti-with reagent
It answers.Reaction vessel after the completion of incubation carries out cleaning operation by cleaning to grab cup mechanism 164 and be transferred in cleaning device 14, with removal
Impurity in reaction vessel.Reaction vessel after cleaning is measured by measuring to grab cup mechanism 165 and be transferred in measuring device 15
Operation, detects the determinand in the reaction vessel after cleaning.
Detection method includes test sample method and system test method, and what above-mentioned detection method illustrated is test sample side
Method is below illustrated system detection method.System testing is divided into background measurement and optical test measurement.Chemiluminescence detector
When carrying out background measurement, it is sent into empty reaction vessel into cleaning device 14, cup mechanism 165 is grabbed by measurement after over cleaning
It is transferred in measuring device 15, measuring device 15 fills substrate solution to reaction vessel, measures background luminous value.Chemiluminescence detection
Instrument is added into reaction vessel in reaction unit 13 when carrying out optical test measurement and quantitative carries answering for luminous marker
Object is closed, is transferred directly in measuring device 15 without over cleaning, measuring device 15 fills substrate solution to reaction vessel, measures its hair
Light value.
Further, detection method further includes following steps:
Before separation cleaning step, the reagent adding step of at least one circulation is repeated, step is mixed and incubates step
Suddenly；And/or
Before measuring process, the reagent adding step of at least one circulation is repeated, step, incubation step is mixed and divides
From cleaning step.
It should be noted that chemiluminescence detector adds number when detecting to sample, according to reagent, it is divided into one
Footwork, two-step method, three-step approach, four step rule.One-step method adds a reagent, and reagent, three-step approach are twice for two-step method i.e. addition
Reagent, four step rule add four reagents three times for addition.It requires to carry out incubation operation after addition reagent every time, moreover, in temperature
Before educating operation, mixing operation can be carried out, can also be operated without mixing.
Also, cleaning operation can be carried out between the adjacent reagent of addition twice, it can also be without cleaning operation；When into
When row cleaning operation, then grabbing cup mechanism 164 by cleaning, the disc mechanism 133 out of react is transferred in cleaning device 14 by reaction vessel
It is cleaned, after the completion of cleaning, then cup mechanism 164 is grabbed by cleaning, reaction vessel is transferred to reaction external disk from cleaning device 14
The cleaning of mechanism 132 puts glass station and carries out subsequent reagent adding operation；When without cleaning operation, cup mechanism 164 is grabbed in cleaning
Reaction vessel in directly reacting in disc mechanism 133, which is transferred to, react the cleaning of outer disc mechanism 132 to put glass station progress subsequent
Reagent adding operation.Also, before measuring device 15 detects the determinand in reaction vessel, it is necessary to pass through cleaning device 14
Separation cleaning operation is carried out, to remove the impurity in reaction vessel, guarantees that 15 testing result of measuring device is accurate and reliable.
In addition, part sample also needs to add pretreating reagent when detecting, to guarantee the accuracy of pattern detection result.This
In pretreating reagent refer to added liquid, solid or mixture etc. before formally addition reagent.Therefore, detection side
Method further includes following steps: before reagent adding step, pretreating reagent being transferred in reaction vessel.That is, detection side
Method further includes adding pretreating reagent step, also, pretreating reagent is stored in reagent storing mechanism, adds pretreating reagent operation by liquid relief
Mechanism 122 realizes.After pipetting device 122 shifts pretreating reagent into reaction vessel, reacts outer disc mechanism 132 and drive reaction vessel
Reagent adding operation and subsequent corresponding operation are executed again after one circle of rotation.It should be noted that the outer disc mechanism 132 of reaction drives
The reaction vessel of pretreating reagent is added during rotation one is enclosed, without mixing step and incubation step.
In the present invention, above-mentioned pattern detection process is only illustrated by taking three-step approach as an example.A kind of situation are as follows: into reaction vessel
After adding sample, after repeating and sequentially execute reagent adding step, mixing step and incubation step three times, to miscellaneous in reaction vessel
Matter carries out separation cleaning operation, then measures operation to the determinand in reaction vessel again.Another situation are as follows: Xiang Fanying
After adding sample in container, repeats and sequence executes reagent adding step, mixes step, incubation step and separation cleaning step 3
It is secondary, operation then is measured to the determinand in reaction vessel again.Another situation are as follows: sample is added into reaction vessel
Afterwards, after sequence executes reagent adding step, mixes step and incubation step, repeat and sequence execute reagent adding step, mix step,
Incubation step and separation cleaning step twice, then measure operation to the determinand in reaction vessel again.
Still further, separation cleaning step carries out the impurity in the reaction vessel after incubation before measuring process
Separation cleaning operates at least once.
That is, chemiluminescence detector is when to pattern detection, it can be in reaction vessel before measuring process
Impurity once cleaned, clean even more be cleaned multiple times twice, can guarantee that the impurity cleaning in reaction vessel is dry in this way
Only, and then guarantee that measuring device 15 measures accurately.It is understood that above said cleaning twice refers to continuous wash twice.
Continuous wash is twice, certainly, also capable of washing primary before measuring process.
Each technical characteristic of embodiment described above can be combined arbitrarily, for simplicity of description, not to above-mentioned reality
It applies all possible combination of each technical characteristic in example to be all described, as long as however, the combination of these technical characteristics is not deposited
In contradiction, it is all considered to be the record scope of this specification.
The embodiments described above only express several embodiments of the present invention, and the description thereof is more specific and detailed, but simultaneously
Limitations on the scope of the patent of the present invention therefore cannot be interpreted as.It should be pointed out that for those of ordinary skill in the art
For, without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to guarantor of the invention
Protect range.Therefore, the scope of protection of the patent of the invention shall be subject to the appended claims.
Claims (18)
1. a kind of chemiluminescence detector characterized by comprising
Reagent storage means are used for storing reagent；The reagent storage means include at least two reagent storing mechanisms；
Reaction unit is operated for carrying reaction vessel and carrying out sample-adding sheet, reagent adding and incubate；The reaction unit has
At least two reagent adding stations；And
Dispenser is used for sample and agent transfer into the reaction vessel；The dispenser include sample charging mechanism and
At least two pipetting devices；Each pipetting device respectively corresponds the reagent storing mechanism and an additional examination
Agent station, the pipetting device is by the agent transfer in the corresponding reagent storing mechanism to the corresponding reagent adding station
The reaction vessel in；Sample is transferred in the reaction vessel by the sample charging mechanism.
2. chemiluminescence detector according to claim 1, which is characterized in that at least two reagent storing mechanism packets
Include the first reagent storing mechanism and the second reagent storing mechanism, at least described reagent adding station includes the first reagent adding station and the
Two reagent adding stations, at least two pipetting devices include the first pipetting device and the second pipetting device；
First pipetting device corresponds to the first reagent storing mechanism and the first reagent adding station setting, is used for institute
The agent transfer in the first reagent storing mechanism is stated into the corresponding reaction vessel of the first reagent adding station；
Second pipetting device corresponds to the second reagent storing mechanism and the second reagent adding station setting, is used for institute
The agent transfer in the second reagent storing mechanism is stated into the corresponding reaction vessel of the second reagent adding station.
3. chemiluminescence detector according to claim 2, which is characterized in that the chemiluminescence detector further includes using
In the reaction vessel automatic conveying device for conveying the reaction vessel, the reaction vessel automatic conveying device is located at the reaction
The side of device；
Also there is the chemiluminescence detector new cup to grab cup mechanism, and the new cup grabs cup mechanism, and to correspond to the reaction vessel automatic
Transmitting device and reaction unit setting, for shifting the reaction vessel in the reaction vessel automatic conveying device
Into the reaction unit.
4. chemiluminescence detector according to claim 1, which is characterized in that the chemiluminescence detector further includes clear
Cleaning device, for removing the impurity in the reaction vessel；The cleaning device is located at the side of the reaction unit；
Also there is the chemiluminescence detector cleaning to grab cup mechanism, and the cleaning grabs cup mechanism and corresponds to the cleaning device and institute
Reaction unit setting is stated, for shifting the reaction vessel between the reaction unit and the cleaning device.
5. chemiluminescence detector according to claim 4, which is characterized in that the chemiluminescence detector further includes surveying
Device is measured, for detecting to the determinand in the reaction vessel；The measuring device is located at the week of the reaction unit
Side, and be disposed adjacent with the cleaning device；
Also there is the chemiluminescence detector measurement to grab cup mechanism, and the measurement grabs cup mechanism and corresponds to the cleaning device and institute
Measuring device setting is stated, for the reaction vessel after cleaning in the cleaning device to be transferred in the measuring device.
6. chemiluminescence detector according to claim 3, which is characterized in that the reaction unit includes buffering disc mechanism
And the reaction disc mechanism independently of the buffering disc mechanism, the buffering disc mechanism accommodate the reaction vessel and execute sample-adding originally
Operation, the reaction disc mechanism accommodate the reaction vessel and execute reagent adding, mixing and incubate operation；
Also there is the chemiluminescence detector sample to grab cup mechanism, the sample grab cup mechanism correspond to the buffering disc mechanism and
The reaction disc mechanism setting, for the reaction vessel on the buffering disc mechanism to be transferred to the reaction disc mechanism
In.
7. chemiluminescence detector according to claim 6, which is characterized in that the reaction disc mechanism includes independent operating
Reaction in disc mechanism with react outer disc mechanism, the outer disc mechanism of the reaction is sheathed in the reaction outside of disc mechanism and altogether
Axis setting；The outer disc mechanism of the reaction accommodates the reaction vessel and executes reagent adding and mix and operates, the reaction inner disc machine
Structure accommodates the reaction vessel and executes incubation operation；
Also there is the chemiluminescence detector incubation to grab cup mechanism, and the incubation grabs cup mechanism and corresponds to disc mechanism in the reaction
It reacts outer disc mechanism with described and is arranged, for disc mechanism with described to react outer disc mechanism in the reaction by the reaction vessel
Between shift.
8. chemiluminescence detector according to claim 7, which is characterized in that the first reagent adding station and described the
Two reagent adding stations are disposed adjacent on disc mechanism, the first reagent storing mechanism is deposited with second reagent outside the reaction
Storage mechanism is symmetrical and is arranged side by side in the side of disc mechanism outside the reaction.
9. chemiluminescence detector according to claim 6, which is characterized in that the chemiluminescence detector further includes sample
This conveying device, for sample to be delivered to sample position；The sample position is located at the buffering side of disc mechanism and right
The sample charging mechanism is answered to be arranged；The sample charging mechanism is drawn sample in sample position and is transferred to described in the buffering disc mechanism
In reaction vessel.
10. chemiluminescence detector according to claim 9, which is characterized in that the sample conveying device includes sample
Storing mechanism and the sample conveying mechanism being detachably connected with the sample storing mechanism, storage is more in the sample storing mechanism
The sample rack is transported in the sample conveying mechanism by a sample rack for being mounted with sample, the sample storing mechanism, and
The sample position is delivered to by the sample conveying mechanism.
11. chemiluminescence detector according to claim 10, which is characterized in that the input terminal of the sample conveying mechanism
It can be docked with the sample storing mechanism, or defeated with the sample conveying mechanism of another chemiluminescence detector
Outlet docking；
The output end of the sample conveying mechanism can be with the sample conveying mechanism of another chemiluminescence detector
Input terminal docking.
12. chemiluminescence detector according to claim 6, which is characterized in that the chemiluminescence detector further includes
Consumption material box loading device, for transmitting the consumptive material the storage box for loading fluid suction head automatically, and the consumption material box loading device can also incite somebody to action
The consumptive material the storage box is delivered to " loaded " position；The sample charging mechanism loads the fluid suction head in the " loaded " position, and passes through
The fluid suction head shifts sample.
13. chemiluminescence detector according to claim 12, which is characterized in that the consumption material box loading device is located at institute
State the side of the separate buffering disc mechanism of reaction vessel automatic conveying device, and the corresponding sample charging mechanism setting.
14. chemiluminescence detector according to claim 5, which is characterized in that the chemiluminescence detector further includes
Pedestal, the pedestal have carrying platform, the reaction unit, the measuring device, the cleaning device, the dispenser
And the reagent storage means are all set on the carrying platform of the pedestal.
15. chemiluminescence detector according to claim 14, which is characterized in that the chemiluminescence detector further includes
Control device and fluid path device；It is the control device and the reaction unit, the measuring device, the cleaning device, described
Dispenser and reagent storage means electrical connection, the fluid path device are connect with the dispenser；The pedestal has
Accommodating space, the accommodating space are located at the lower section of the pedestal, and the control device and fluid path device are set to the accommodating
In space.
16. a kind of detection method of chemiluminescence detector, which comprises the steps of:
It is loaded this step；Sample is transferred in reaction vessel；
Reagent adding step；By agent transfer into the reaction vessel；
Mix step；Mixture in the reaction vessel is uniformly mixed；
Incubation step；Incubation operation is carried out to the mixture being uniformly mixed in the reaction vessel；
Separation cleaning step；The impurity in the reaction vessel after removal incubation；
Measuring process；Determinand in the reaction vessel after cleaning is detected.
17. detection method according to claim 16, which is characterized in that the detection method further includes following steps:
Before the separation cleaning step, the reagent adding step, the mixing step of at least one circulation are repeated
And the incubation step；And/or
Before the measuring process, the reagent adding step, the mixing step, described of at least one circulation is repeated
Incubation step and the separation cleaning step.
18. detection method according to claim 16 or 17, which is characterized in that before the measuring process, described point
Separation cleaning at least once is carried out to the impurity in the reaction vessel after incubation from cleaning step to operate.
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