CN111373265A

CN111373265A - Chemiluminescence analyzer and analysis method thereof

Info

Publication number: CN111373265A
Application number: CN201880074717.7A
Authority: CN
Inventors: 何崇东; 谢良; 王俊
Original assignee: Shenzhen Mindray Bio Medical Electronics Co Ltd
Current assignee: Shenzhen Mindray Bio Medical Electronics Co Ltd
Priority date: 2018-03-26
Filing date: 2018-03-26
Publication date: 2020-07-03
Also published as: WO2019183745A1

Abstract

A chemiluminescence analyzer and an analysis method thereof, the analyzer comprises a sample loading device (01), a reagent loading device (02), a dispensing device (03), a blending device (04), a sample incubation device (05), a magnetic separation cleaning device (06), a sample detection device (07) and a transfer cup grabbing device (08); the sample incubation device (05) is respectively arranged adjacent to the blending device (04), the magnetic separation cleaning device (06) and the sample detection device (07); the transferring cup grabbing device (08) transfers the reaction container to the blending device (04); the dispensing device (03) transfers the sample and the reagent to a reaction container of the mixing device (04) respectively; the transfer cup grabbing device (08) transfers the reaction container from the blending device (04) to the sample incubation device (05) for incubation, the transfer cup grabbing device (08) also transfers the incubated reaction container to the magnetic separation cleaning device (06) for separation and cleaning, and transfers the separated and cleaned reaction container to the incubation photometric device for luminescence detection. The expansion of the loading capacity of the sample and the reagent is facilitated, so that the chemiluminescence analyzer is simple in structure and small in occupied space.

Description

Chemiluminescence analyzer and analysis method thereof

Technical Field

The invention relates to the technical field of chemiluminescence detection, in particular to a chemiluminescence analyzer and an analysis method thereof.

Background

The chemiluminescence immunoassay technology is a high-sensitivity and high-specificity analysis instrument which develops rapidly in the world in the last decade, and is used for detecting various immunity indexes of blood, urine or other body fluids in a clinical laboratory. The main operation flow in the chemiluminescence analyzer comprises sample loading, reagent loading, sample and reagent separate injection, reaction liquid mixing, reaction liquid incubation, magnetic separation, cleaning and separation, substrate luminescence liquid injection and photometry. At present, a chemiluminescence analyzer loads a sample and a reagent, the sample is stored by adopting an inner disc and an outer ring to reduce the size, but the chemiluminescence analyzer adopts a disc and ring structure, has a complex structure and is inflexible to expand, and cannot meet the use requirement.

Disclosure of Invention

Therefore, it is necessary to provide a chemiluminescence analyzer which can facilitate expansion of the loading capacity of a sample and a reagent and can reduce the volume of the whole chemiluminescence analyzer, and an analysis method applied to the chemiluminescence analyzer, aiming at solving the problem that the current chemiluminescence analyzer cannot be expanded due to the fact that the reagent is contained in an inner tray and the sample is contained in an outer tray.

The above purpose is realized by the following technical scheme:

a chemiluminescence analyzer comprises a sample loading device for storing samples, a reagent loading device for storing reagents, a separate injection device for sucking and discharging samples and reagents, a uniform mixing device for supporting reaction vessels, a sample incubation device for incubation, a magnetic separation and cleaning device for separation and cleaning, a sample detection device for luminescence detection and a transfer cup grabbing device for transferring the reaction vessels;

the sample loading device and the reagent loading device are arranged side by side, the sample incubation device is respectively arranged adjacent to the blending device, the magnetic separation cleaning device and the sample detection device, the sample incubation device, the blending device, the magnetic separation cleaning device and the sample detection device are positioned on the same side of the sample loading device, and the transfer cup grabbing device can move to the positions above the blending device, the sample incubation device, the magnetic separation device and the sample detection device;

the transferring cup grabbing device transfers the reaction container to the blending device; the dispensing device can move above the sample loading device and the reagent loading device and can respectively transfer the sample and the reagent into a reaction container of the blending device; the transfer grab the glass device will reaction vessel follows mixing device transfers to incubate in the device is hatched to the sample, transfer grab the glass device still will incubate after reaction vessel transfers to magnetic separation belt cleaning device separates the washing, and will separate after the washing reaction vessel transfer to it carries out luminescence detection to incubate among the photometric device.

In one embodiment, the chemiluminescence analyzer further comprises a bearing platform, the sample loading device and the reagent loading device are arranged on the right side of the bearing platform side by side, the magnetic separation cleaning device, the sample detection device, the sample incubation device and the blending device are arranged on the left rear side of the bearing platform, the blending device is arranged on the right side of the sample incubation device, the separate injection device is arranged on the right rear side of the bearing platform, and the transfer cup grabbing device is arranged on the left front side of the bearing platform.

In one embodiment, the number of the blending devices is at least two, at least two blending devices are arranged side by side and are driven independently, and when one blending device is used for adding a sample and a reagent, the other blending devices can drive the reaction container to perform blending operation.

In one embodiment, the sample incubation device comprises an incubation block and a heating member disposed below the incubation block, wherein the heating member is configured to heat the incubation block, the incubation block is provided with a plurality of incubation holes arranged in an array, the incubation holes are configured to place the reaction containers and perform incubation, and the incubation holes are further configured to temporarily store the reaction containers after detection.

In one embodiment, the chemiluminescence analyzer has a photometric well, which is disposed independently from the sample incubation device and is used for carrying the reaction container to be detected, and the sample detection device is disposed at the photometric well and is used for performing luminescence detection on the reaction container in the photometric well.

In one embodiment, the chemiluminescence analyzer is provided with a light measuring hole, the sample incubation device comprises an incubation block, the light measuring hole is positioned at the edge position of the incubation block, and the sample detection device is arranged on the side surface of the incubation block and corresponds to the light measuring hole.

In one embodiment, the chemiluminescent analyzer further comprises an opening and closing door, wherein the opening and closing door can be opened and closed to cover the photometric hole, and the opening and closing door closes the photometric hole when the sample detection device performs luminescence detection on the reaction container in the photometric hole.

In one embodiment, the sample incubation device includes an incubation block, the incubation block has a fixing dilution hole, the fixing dilution hole is disposed adjacent to the mixing device, the fixing dilution hole is used for bearing the reaction container to be diluted, the dispensing device transfers the mixture in the reaction container in the fixing dilution hole to the reaction container of the mixing device, and the dispensing device transfers the reagent to dilute the sample.

In one embodiment, the mixing device and the reagent loading device are respectively arranged at two sides of the sample loading device, or the mixing device and the sample loading device are respectively arranged at two sides of the reagent loading device;

the reagent loading device comprises a plurality of reagent boxes arranged in an array, and each reagent box is used for bearing a plurality of reagent containers.

In one embodiment, the reagent loading device further comprises a reagent storage transmission mechanism and a reagent storage driving mechanism, wherein a plurality of reagent boxes are arranged in two rows and are respectively located on two sides of the reagent storage transmission mechanism, and each reagent box is provided with a mixing position and a rest position and is used for respectively bearing the reaction container; the reagent storage transmission mechanism is in transmission connection with the reagent storage driving mechanism and the plurality of reagent boxes and drives the reagent containers on the mixing positions of the reagent boxes to rotate so as to mix the reagents in the reagent containers.

In one embodiment, the reagent storage driving mechanism comprises a reagent storage transmission structure, a blending part with a tooth part and a plurality of blending gear chassis, the reagent storage transmission structure is connected with the blending part, the blending gear chassis is positioned on the blending position of the reagent kit and bears the reagent container, the blending part is in transmission connection with the blending gear chassis on two sides through the tooth part, and the reagent storage transmission structure drives the blending gear chassis to rotate through the blending part so as to blend the reagents in the reagent container.

In one embodiment, the reagent storage transmission mechanism comprises a reagent storage transmission structure, two blending parts with tooth parts and a plurality of blending gear chassis, the two blending parts are respectively connected with the reagent storage transmission structure, the blending gear chassis is positioned on the blending position of the reagent kit and bears the reagent container, the two blending parts are respectively in transmission connection with the corresponding blending gear chassis through the tooth parts, and the reagent storage transmission structure drives the blending gear chassis to rotate through the blending parts so as to blend the magnetic reagent in the reagent container.

In one embodiment, the sample loading device comprises a plurality of sample racks arranged side by side, each sample rack carrying a plurality of sample containers.

In one embodiment, the chemiluminescence analyzer further comprises a bearing platform and a cleaning pool, the cleaning pool is located on one side, away from the incubation block, of the blending device, the cleaning pool is located on the rear side of the sample loading device, and the cleaning pool is used for cleaning the separate injection device.

In one embodiment, the chemiluminescent analyzer further comprises a substrate injection mechanism for adding a substrate to the reaction vessel;

the substrate injection mechanism is arranged on the magnetic separation cleaning device, or the substrate injection mechanism is arranged on the sample incubation device.

In one embodiment, the chemiluminescence analyzer further comprises a bearing platform, a substrate bearing part and a substrate injection mechanism, wherein the substrate bearing part is arranged on the bearing platform and is positioned at the right side edge of the bearing platform, the substrate bearing part bears a substrate container, and the substrate in the substrate container is sucked by the substrate injection mechanism and is conveyed into the reaction container.

In one embodiment, the chemiluminescence analyzer further comprises a reaction container loading device, wherein the reaction container loading device is arranged on the carrying platform and is positioned at the front side of the sample incubation device and used for carrying the reaction container; the transferring cup grabbing device can move to the position above the reaction container loading device to grab the reaction container in the reaction container loading device and transfer the reaction container to the blending device.

In one embodiment, the chemiluminescence analyzer further comprises a waste bin, wherein the waste bin is arranged on the bearing platform and positioned at the right side of the reaction container loading device and used for recovering the detected reaction containers; the transfer cup grasping device is movable to above the waste bin to grasp the reaction vessel at the sample incubating device and transfer to the waste bin.

An analysis method of a chemiluminescence analyzer comprises a sample loading device, a reagent loading device, a separate injection device, a mixing device, a sample incubation device, a magnetic separation cleaning device, a sample detection device and a transferring and cup grabbing device, wherein the mixing device comprises a first mixing device and a second mixing device; the analysis method comprises the following steps:

a step of adding the cup, in which the transferring cup grabbing device transfers the empty reaction container to the first blending device and the second blending device;

adding a sample, wherein the separate injection device transfers the sample in the sample loading device to a reaction container of the first mixing device;

a reagent adding step of transferring the reagent in the reagent storage device to a reaction vessel of the first mixing device by the dispensing device;

uniformly mixing, namely uniformly mixing the sample and the reagent in the reaction container by using the first uniformly mixing device; simultaneously, executing the sample adding step and the reagent adding step on the second blending device;

an incubation step, wherein the transfer cup grabbing device transfers the uniformly mixed reaction container to a sample incubation device, and the sample incubation device incubates the uniformly mixed mixture in the reaction container;

a magnetic separation cleaning step, wherein the incubated reaction vessel is transferred to a magnetic separation cleaning device by the transfer cup grabbing device, and impurities in the incubated reaction vessel are removed by the magnetic separation cleaning device;

and a detection step, wherein the cleaned reaction container is transferred to the sample detection device by the transfer cup grabbing device, and the sample detection device performs luminescence detection on the cleaned object to be detected in the reaction container.

In one embodiment, the analysis method further comprises the steps of:

and after the uniformly mixing step or after the magnetic separation and cleaning step, performing the reagent adding step at least once again.

In one embodiment, the analysis method further comprises the steps of:

prior to the incubation step, a dilution step is performed, the dilution step being used to dilute the sample.

In one embodiment, the diluting step comprises the steps of:

the transferring cup grabbing device transfers the empty reaction container to the second blending device;

the dispensing device transfers a mixture portion in the reaction vessel in the first kneading device to the reaction vessel in the second kneading device;

and (c) performing the reagent adding step on the reaction vessel in the second blending device.

In one embodiment, the chemiluminescent analyzer also has a wash tank; before and after the sample adding step and before and after the reagent adding step, the analysis method further comprises a washing step, and the dispensing device moves to the washing pool for washing.

In one embodiment, when the reagent container is loaded with the magnetic particle reagent, the analysis method further comprises a reagent blending step for blending the magnetic particle reagent in the reagent container before the reagent adding step.

After the technical scheme is adopted, the invention has the beneficial effects that:

when the chemiluminescence analyzer detects a sample, a dispensing device sucks the sample in a sample loading device and transfers the sample to a reaction container, the dispensing device also transfers a reagent in a reagent loading device to the reaction container, then transfers the reaction container to an incubation photometric device for incubation operation, after the incubation is finished, transfers the reaction container to a magnetic separation cleaning device, separates and cleans the reaction container, after the cleaning is finished, transfers the reaction container to a sample detection device, and performs luminescence detection on the reaction container to obtain various parameters of the sample; the sample loading device and the reagent loading device of the chemiluminescence analyzer are independently arranged, so that the problem that the conventional chemiluminescence analyzer cannot be expanded due to the fact that the reagent is contained in the inner tray and the sample is contained in the outer tray can be effectively solved, the expansion of the loading capacity of the sample and the reagent is facilitated, and the use requirement is met. In addition, each part of the chemiluminescence analyzer executes the steps according to the arrangement mode, so that the chemiluminescence analyzer is simple in structure and convenient to operate, the size of the whole chemiluminescence analyzer can be reduced, the occupied space is small, the production cost is reduced, the chemiluminescence analyzer is easy to realize miniaturization development, and the chemiluminescence analyzer is convenient for operators to use.

Drawings

FIG. 1 is a schematic top view of a chemiluminescent analyzer according to one embodiment of the present invention;

FIG. 2 is a schematic top view of the reagent loading device of the chemiluminescence analyzer shown in FIG. 1;

FIG. 3 is a schematic top view of another embodiment of a reagent loading device in the chemiluminescence analyzer shown in FIG. 1;

FIG. 4 is a layout of another embodiment of the sample loading device, reagent loading device and substrate carrier device of the chemiluminescent analyzer of FIG. 1;

FIG. 5 is a schematic top view of a chemiluminescent analyzer of another embodiment of the present invention;

fig. 6 is a schematic top view of a chemiluminescent analyzer according to still another embodiment of the present invention.

Wherein:

01-a sample loading device;

02-reagent loading device; 021-kit; 022-reagent storage drive mechanism; 0221-mixing gear chassis; 0222-mixing part;

03-a dispensing device;

04-uniformly mixing device; 041-a first blending device; 042-a second blending device;

05-a sample incubation device;

06-magnetic separation cleaning device;

07-a sample detection device;

08-transferring cup grabbing device;

09-photometry hole;

10-a cleaning pool;

11-a load-bearing platform;

12-a substrate injection mechanism;

13-a substrate carrier;

14-reaction vessel loading means;

15-a waste bin;

16-fixed dilution wells.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more clearly understood, the chemiluminescence analyzer and the analysis method thereof according to the present invention are further described in detail below by way of examples with reference to the accompanying drawings. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.

The numbering of the components as such, e.g., "first", "second", etc., is used herein only to distinguish the objects as described, and does not have any sequential or technical meaning. The term "connected" and "coupled" when used in this application, unless otherwise indicated, includes both direct and indirect connections (couplings). In the description of the present invention, it is to be understood that the terms "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", and the like, indicate orientations or positional relationships based on those shown in the drawings, and are only for convenience of description and simplicity of description, but do not indicate or imply that the referenced devices or elements must have a particular orientation, be constructed and operated in a particular orientation, and thus, are not to be construed as limiting the present invention.

In the present invention, unless otherwise expressly stated or limited, the first feature "on" or "under" the second feature may be directly contacting the first and second features or indirectly contacting the first and second features through an intermediate. Also, a first feature "on," "over," and "above" a second feature may be directly or diagonally above the second feature, or may simply indicate that the first feature is at a higher level than the second feature. A first feature being "under," "below," and "beneath" a second feature may be directly under or obliquely under the first feature, or may simply mean that the first feature is at a lesser elevation than the second feature.

Referring to fig. 1, the present invention provides a chemiluminescence analyzer, which is used for analyzing and detecting a sample to be detected to obtain a corresponding detection result, so as to meet the use requirement. It should be noted that the specific type of sample to be tested is not limited, and in some embodiments, the sample to be tested includes a solid sample or a liquid sample. It can be understood that the liquid sample is detected by carrying the liquid sample through a container such as a test tube and placing the liquid sample on a sample rack. Further liquid samples include, but are not limited to, blood samples. When the chemiluminescence analyzer is used for detecting blood samples, the blood samples are stored in test tubes and are sequentially placed on a test tube rack. The chemiluminescence analyzer is simple in structure and convenient to operate, and meanwhile, the size of the whole chemiluminescence analyzer can be reduced, so that the occupied space is small, the production cost is reduced, the chemiluminescence analyzer is easy to realize miniaturization development, and the chemiluminescence analyzer is convenient for operators to use.

In the present invention, the chemiluminescence analyzer includes a sample loading device 01, a reagent loading device 02, a dispensing device 03, a mixing device 04, a sample incubation device 05, a magnetic separation and cleaning device 06, a sample detection device 07, and a transfer cup grasping device 08. The sample loading device 01 is used to carry sample containers. It is understood that the sample in the sample loading device 01 can be added manually by an operator, and the sample can be added automatically using an automatic sample feeding structure. The reagent loading device 02 is used for storing reagents, namely, the reagent loading device 02 loads various reagents required by sample detection, so that the required reagents can be conveniently selected, and the reagent sucking efficiency is improved. The dispensing device 03 is used for sucking and discharging the sample and the reagent to realize the transfer of the sample or the reagent into the reaction vessel. The mixing device 04 is used for supporting the reaction container and mixing the sample and the reagent in the reaction container. It is understood that the empty reaction vessel is transferred to the mixing device 04, the dispensing device 03 transfers the sample and the reagent to the reaction vessel, and the reaction vessel is transferred to the sample incubation device 05 after the sample and the reagent are uniformly mixed by the mixing device 04. The sample incubation device 05 is used for incubation, i.e. incubation operation of the sample and the reagent in the reaction vessel, and the magnetic separation and cleaning device 06 is used for separation and cleaning, i.e. cleaning of the sample and the reagent in the reaction vessel after incubation, so as to remove impurities. The sample detection device 07 is used for luminescence detection, that is, luminescence detection is performed on the sample and the reagent in the reaction container after cleaning, so as to obtain each parameter of the sample. After the reaction container is transferred to the sample incubation device 05, the sample incubation device 05 can incubate the sample and the reagent in the reaction container, the incubated reaction container is transferred to the magnetic separation cleaning device 06 for separation and cleaning, and the cleaned reaction container is transferred to the sample detection device 07 for luminescence detection to obtain the corresponding parameters of the sample. Moreover, the transfer cup grasping device 08 is used for transferring reaction vessels, and particularly, the transfer cup grasping device 08 can transfer reaction vessels among the blending device 04, the sample incubation device 05, the magnetic separation cleaning device 06 and the sample detection device 07.

To facilitate understanding of the names of the various stages of the sample and reagent, the names of the various stages of the sample and reagent are described in detail herein: the mixture of the sample and the reagent in the reaction container is called a mixture, and the sample incubation device 05 can incubate the mixture in the reaction container to fully react the sample and the reagent, in which case, the substances in the reaction container are the analyte and the impurities. The mixture is a substance formed by mixing the sample and the reagent, and is referred to as a mixture herein regardless of the ratio and concentration of the sample and the reagent. The incubated mixture is presented in the reaction vessel as the analyte and impurities. The impurities may be insufficiently reacted substances, side reaction products generated by side reactions, other substances affecting the detection of the sample incubation apparatus 05, or the like, or a combination of at least two of the foregoing. The magnetic separation cleaning device 06 cleans the substance to be measured and the impurities in the reaction vessel to remove the impurities in the reaction vessel, so that only the substance to be measured exists in the reaction vessel. The sample detection device 07 can detect the analyte in the reaction container to obtain various parameters of the sample. If the substrate is added into the separated and cleaned reaction container, namely the substrate is mixed with the object to be detected, the substrate does not change the attribute of the object to be detected, and only the luminous value of the object to be detected is increased, so that the object to be detected is still called after the substrate is mixed with the object to be detected.

The sample loading device 01 is arranged side by side with the reagent loading device 02. When the sample loading unit 01 and the reagent loading unit 02 are separately provided, the structures therebetween are not affected by each other. That is, the number of sample containers carried by the sample loading device 01 can be increased according to the use requirement, that is, when the detection requirement is large, the number of sample containers to be placed is increased; when the sample detection requirement is small, the number of sample containers is reduced, so that the sample loading device 01 has high expandability and meets different use requirements of users. In the present embodiment, the sample loading device 01 carries genre sample containers, and the number of rows of sample containers can be expanded accordingly according to actual situations. Accordingly, the number of reagent containers carried by the reagent loading device 02 can be increased according to the use requirement, the reagent loading device 02 with larger capacity can be replaced, and reagent containers can be directly added at the edge of the reagent loading device 02 as long as the reagent containers are ensured within the movement stroke range of the dispensing device 03.

The sample incubation device 05 is respectively arranged adjacent to the blending device 04, the magnetic separation cleaning device 06 and the sample detection device 07, and the sample incubation device 05, the blending device 04, the magnetic separation cleaning device 06 and the sample detection device 07 are positioned on the same side of the sample loading device 01. Specifically, the blending device 04, the magnetic separation cleaning device 06, and the sample detection device 07 are disposed around the sample incubation device 05. The transfer cup grabbing device 08 can move to the positions above the blending device 04, the sample incubation device 05, the magnetic separation device and the sample detection device 07. Therefore, when the transfer cup grabbing device 08 transfers the reaction container, the reaction container can move according to the shortest movement path, if the transfer cup grabbing device 08 transfers the reaction container on the blending device 04 to the sample incubation device 05, transfers the reaction container in the sample incubation device 05 to the magnetic separation cleaning device 06, and transfers the reaction container in the magnetic separation cleaning device 06 to the sample incubation device 05 or the sample detection device 07, so as to shorten the transfer path of the reaction container, improve the processing efficiency, and further improve the processing efficiency of chemiluminescence analysis. Moreover, when the above mode is adopted to distribute the structures of the chemiluminescence analyzer, the whole chemical luminescence analyzer is reasonable and compact in layout and small in size, and can be conveniently operated and used by a user and maintained.

The dispensing device 03 can move above the sample loading device 01 and the reagent loading device 02, and the dispensing device 03 can also move to the mixing device 04, so that the transfer of the sample and the reagent is realized. The sample loading device 01 has a sample sucking station, the reagent loading device 02 has a reagent sucking station, the dispensing device 03 sucks the sample in the sample container in the sample loading device 01 at the sample sucking station and transfers the sample to the reaction container of the mixing device 04, and the dispensing device 03 also sucks the reagent in the reagent container in the reagent storage device at the reagent sucking station and transfers the reagent to the reaction container of the mixing device 04. It will be appreciated that there is no requirement for the order in principle of sample and reagent transfer, i.e. the sample may be transferred first and the reagent may be transferred second, or the reagent may be transferred first and the sample may be transferred second.

The transferring cup grabbing device 08 transfers the reaction container to the mixing device 04; the separate injection device 03 transfers the sample and the reagent to a reaction container of the mixing device 04 respectively, and the sample and the reagent are mixed by the mixing device 04; the transfer cup grabbing device 08 transfers the reaction container from the blending device 04 to the sample incubation device 05 for incubation, the transfer cup grabbing device 08 also transfers the incubated reaction container to the magnetic separation cleaning device 06 for separation and cleaning, and transfers the separated and cleaned reaction container to the incubation photometric device for luminescence detection. Specifically, the transferring and cup-grasping device 08 transfers an empty reaction container to the blending device 04, then the dispensing device 03 respectively adds a sample and a reagent to the reaction container, then the sample and the reagent in the reaction container are uniformly mixed on the blending device 04 to form a mixture, then the transferring and cup-grasping device 08 transfers the reaction container from the blending device 04 to the sample incubation device 05, and the sample incubation device 05 incubates the mixture in the reaction container, so that the mixture forms an object to be measured and impurities in the reaction container; then the transfer cup grabbing device 08 transfers the reaction container from the sample incubation device 05 to the magnetic separation cleaning device 06, and the magnetic separation cleaning device 06 cleans and removes impurities in the reaction container to leave an object to be detected; the transfer cup grabbing device 08 transfers the cleaned reaction container to the sample detection device 07, and the sample detection device 07 detects the object to be detected in the reaction container to obtain various parameters of the sample.

Specifically, the transfer cup grabbing device 08 is provided with a moving mechanism moving along the XYZ direction and a cup grabbing hand, the cup grabbing hand is arranged on the moving mechanism, the moving mechanism can drive the cup grabbing hand to move at any position of the three-dimensional space, the reaction container can be grabbed and released at any position of the three-dimensional space, and then the reaction container can be transferred. Specifically, the moving mechanism drives the cup grabbing hand to grab an empty reaction container at the reaction container loading device 14 and transfer the empty reaction container to the mixing device 04, the reaction container in the mixing device 04 can be transferred to the sample incubation device 05, the reaction container in the sample incubation device 05 can be transferred to the magnetic separation cleaning device 06 or the sample incubation mechanism, the reaction container in the magnetic separation cleaning device 06 can be transferred to the mixing device 04 or the sample detection device 07, and the like. The motion mechanism comprises a synchronous belt structure which is matched with a motor to move in the three directions of XYZ so as to realize the motion in the corresponding direction, and of course, the synchronous belt structure can also be replaced by a gear rack structure, a chain transmission structure or other structures capable of realizing linear motion.

The chemiluminescence analyzer is used for detecting the luminous value of an object to be detected so as to obtain various parameters of a sample. In order to increase the luminous value of the object to be detected during detection, the chemiluminescence analyzer disclosed by the invention adds the substrate into the reaction container after separation and cleaning, and the substrate is attached to the object to be detected, so that the luminous value of the object to be detected can be increased, and the accuracy of sample detection is ensured. Specifically, a substrate container is placed in the chemiluminescence analyzer, the substrate container is used for containing a substrate, and the substrate is added into the reaction container through a liquid path. Therefore, the structural arrangement of the bearing reaction container when adding the substrate can be reduced, the transfer times of the reaction container are reduced, and the volume of the whole machine is further reduced. After magnetic separation and cleaning, adding a substrate into the reaction container to mix the substrate with the object to be detected, transferring the reaction container from the magnetic separation cleaning device 06 to the sample incubation device 05, transferring the reaction container to the sample monitoring device by the transferring cup grabbing device 08 after incubation, and performing luminescence detection on the object to be detected by the sample detection device 07 to obtain various parameters of the sample.

As an embodiment, the chemiluminescence analyzer further comprises a carrying platform 11. The sample loading device 01 and the reagent loading device 02 are located on the right side of the bearing platform 11 side by side, the magnetic separation cleaning device 06, the sample detection device 07, the sample incubation device 05 and the blending device 04 are located on the left rear side of the bearing platform 11, the blending device 04 is located in the middle area of the bearing platform 11, the separate injection device 03 is located on the right rear side of the bearing platform 11, and the transfer cup grabbing device 08 is located on the left front side of the bearing platform 11. The bearing platform 11 plays a bearing role and is supported by a bracket, so that a placing space is provided for each structure of the full-automatic chemiluminescence immunoassay analyzer. Specifically, the sample loading device 01, the reagent loading device 02, the magnetic separation cleaning device 06, the sample detection device 07, the sample incubation device 05 and the blending device 04 are all arranged on the bearing platform 11, and a liquid path, an electrical component and the like of chemiluminescence analysis are all arranged below the bearing platform 11, so that the space can be fully utilized, and the whole volume of the chemiluminescence analyzer is small.

It is understood that the side of the chemiluminescent analyzer operated by the user is defined as the front side of the carrier platform 11, correspondingly, the side opposite to the front side of the carrier platform 11 is the rear side of the carrier platform 11, and the two sides adjacent to the front side of the carrier platform 11 are the left and right sides of the carrier platform 11. Specifically, as shown in fig. 1, the supporting platform 11 has a left side, a right side, a front side and a rear side, the right side of the supporting platform 11 is a sample reagent management area, the left side is a reaction container scheduling reaction detection area, and the rear side is an auxiliary supporting area. Wherein the substrate container is placed on the rightmost edge of the right sample reagent management area, and the sample loading device 01 and the reagent loading device 02 are disposed in the right sample reagent management area and on the left side of the substrate container. The dispensing device 03 is located above the rear side of the sample reagent management area, and the dispensing device 03 is movable above the sample reagent management area to respectively aspirate the sample at the sample loading device 01 and the reagent at the reagent loading device 02. It is understood that the mixing device 04 and the reagent loading device 02 are provided on both sides of the sample loading device 01, or the mixing device 04 and the sample loading device 01 are provided on both sides of the reagent loading device 02. That is, the left and right positions of the sample loader 01 and the reagent loader 02 are not limited, that is, the sample loader 01 may be on the left, the reagent loader 02 may be on the right, or the reagent may be loaded on the left, the sample loader 01 may be on the right, and the sample loader 01 and the reagent loader 02 may be arranged side by side. In the present embodiment, the sample loading device 01 is located on the right side of the reagent loading device 02.

The left side of load-bearing platform 11 is reaction vessel scheduling reaction detection area, and device 05, mixing device 04, magnetic separation belt cleaning device 06, sample detection device 07 and transport and grab a glass device 08 and all set up at left reaction vessel scheduling reaction detection area. The transfer cup grabbing device 08 is located on the left front side of the bearing platform 11, and the movement area of the transfer cup grabbing device 08 can cover a reaction container scheduling reaction detection area, so that the transfer of the reaction container is realized. In this embodiment, mixing device 04 is located the right side that device 05 was hatched to the sample, magnetic separation belt cleaning device 06 is located the rear side that device 05 was hatched to the sample with sample detection device 07, thus, use device 05 is hatched to the sample as the reaction scheduling center, form a little overall arrangement, when transferring and grabbing cup device 08 and transferring reaction vessel at mixing device 04, device 05 is hatched to the sample, magnetic separation belt cleaning device 06 and sample detection device 07 between, can shorten reaction vessel's transfer route, improve transfer efficiency, and can also reduce occupation space, and then reduce the complete machine volume. Preferably, the blending device 04 and the sample incubation device 05 are arranged side by side, and the magnetic separation and cleaning device 06 and the sample detection device 07 are arranged side by side. The rear part and the bottom of the bearing platform 11 are provided with a gas-liquid path and a circuit system for supporting the whole machine to operate, and the purpose of the arrangement is as follows: parts which are possibly required to be maintained are placed on the periphery of the whole machine as far as possible, and the maintenance complexity which possibly appears at a client in the future is reduced.

Optionally, the chemiluminescence analyzer further comprises a reaction container loading device 14, wherein the reaction container loading device 14 is disposed on the carrying platform 11, and the reaction container loading device 14 is located at the front side of the sample incubation device 05 and is used for carrying a reaction container; the transfer cup grasping device 08 can be moved above the reaction vessel loading device 14 to grasp the reaction vessel in the reaction vessel loading device 14 and transfer to the kneading device 04. Specifically, the reaction container loading device 14 is located in the reaction container scheduling reaction detection area, and is located on one side of the sample incubation device 05 away from the magnetic separation cleaning device 06, so as to bear the reaction container and improve the conveying efficiency. Of course, in other embodiments of the present invention, the reaction container loading device 14 may be replaced, that is, the reaction container may be directly placed in the sample incubation device 05 without using the reaction container loading device 14 to transport the reaction container. Preferably, the reaction vessels transported by the reaction vessel loader 14 are usually disposable consumables, but they can also be recycled. Alternatively, when the reaction vessel is reused, the reaction vessel may be transported without using the reaction vessel loading device 14. Also, the reaction container means a consumable material such as a reaction cup, a test tube, a sample slide, a sample tube, etc. which carries and enables a sample reaction, a detection analysis, and the like. In this embodiment, the reaction vessel means a reaction cup.

Optionally, the reaction container loading device 14 of the present invention includes a tray structure, on which the reaction containers are placed in an ordered arrangement, so as to facilitate the transportation of the cup grasping device 08 to grasp the reaction containers. Preferably, the number of reaction vessel loaders 14 is two, and two reaction vessel loaders 14 are arranged side by side. The two reaction vessel loaders 14 may be used interchangeably, i.e., to support changing tray structures during testing. After the reaction container in one of the reaction container loading devices 14 is grabbed, the tray structure needs to be taken out to load and fill the reaction container, and at this time, the other reaction container loading device 14 can continue to provide the reaction container for the chemiluminescence analyzer, so that the influence on the operation of the chemiluminescence analyzer due to the no-load of the reaction container loading device is avoided, the chemiluminescence analyzer can continuously perform sample detection, and the efficiency is improved. Of course, in other embodiments of the present invention, the reaction vessel loading device 14 may also be a drawer structure.

Optionally, the chemiluminescence analyzer of the present invention uses a disposable reaction vessel for sample detection, and after the luminescence detection is completed, the reaction vessel after use needs to be recovered. The chemiluminescence analyzer also comprises a waste box 15, wherein the waste box 15 is arranged on the bearing platform 11, is positioned at the right side of the reaction container loading device 14 and is used for recovering the detected reaction containers; the transfer cup grasping device 08 can be moved above the waste bin 15 to grasp the reaction vessel at the sample incubation device 05 and transfer into the waste bin 15. Specifically, the top of the waste bin 15 is provided with an opening, and the transferring cup-grasping device 08 can enable the detected reaction container to enter the waste bin 15 through the gap of the waste bin 15. The waste bin 15 can retrieve the reaction vessel after using in succession, avoids occuping the position in the sample incubation device 05, can also avoid the reaction vessel after using to be thrown away in disorder simultaneously. After the waste bin 15 is filled with reaction vessels or the reaction vessels in the waste bin 15 need to be emptied, the waste bin 15 can be removed from the chemiluminescence analyzer and the waste bin 15 can be installed back on the complete chemiluminescence analyzer after being emptied.

As an embodiment, the chemiluminescence analyzer further comprises a main control device and a power supply device, wherein the power supply device is electrically connected with the main control device, the main control device is electrically connected with the sample loading device 01, the reagent loading device 02, the separate injection loading and mixing device 04, the sample incubation device 05, the magnetic separation cleaning device 06, the sample detection device 07, the transfer cup grasping device 08, the reaction vessel loading device 14, and the like, and the main control device and the power supply device are positioned below the carrying platform 11. A software control system is integrated in the main control device, and the mutual matching movement of all parts of the chemiluminescence analyzer is realized through the software control system, so that the operating efficiency of the full-automatic chemiluminescence immunoassay analyzer is improved. The main control device is arranged below the bearing platform 11 and can reduce the volume of each part, and the space occupied on the bearing platform 11 is greatly reduced, so that the structure of the chemiluminescence analyzer is compact, and the miniaturization trend of the chemiluminescence analyzer is facilitated. In addition, the main control device integrates the control of each part, so that the maintenance operation is convenient, and the cost and the failure rate of the machine can be reduced.

Optionally, the chemiluminescent analyzer also includes a substrate injection mechanism 12, the substrate injection mechanism 12 for adding substrate to the reaction vessel. When it is necessary to add a substrate to the reaction vessel to increase the light emission value, this is achieved by the substrate injection mechanism 12. It should be noted that the injection of the substrate is performed after the magnetic separation cleaning device 06 finishes cleaning, so as to avoid the substrate from being combined with impurities, and the substrate and the object to be detected are fully reacted after being incubated by the sample incubation device 05, so as to facilitate the luminescence detection of the sample. Alternatively, the substrate injection mechanism 12 includes a substrate mounting seat and a substrate needle, the substrate needle is mounted on the substrate mounting seat, one end of the substrate needle is connected to the substrate container through a liquid path, and the reaction container is transferred to the lower side of the substrate needle to add the substrate in the substrate container to the reaction container.

Referring to FIG. 1, in one embodiment, the substrate injection mechanism 12 is disposed on the magnetic separation cleaning device 06. That is, after the completion of the washing of the mixture in the reaction vessel, the substrate is directly added to the magnetic separation washing apparatus 06. Referring to fig. 5, of course, in another embodiment, the substrate injection mechanism 12 is disposed on the sample incubation device 05. That is, the sample incubation device 05 has a substrate injection hole, after the transfer cup grasping device 08 transfers the washed reaction container to the substrate of the sample incubation device 05, the substrate injection mechanism 12 adds the substrate to the reaction container, and after the transfer cup grasping device 08 transfers the reaction container to another position of the sample incubation device 05 for incubation, because the sample incubation time is long, in order to avoid delaying the subsequent sample injection of the substrate, the reaction container to which the substrate is added is transferred away. It can be understood that the substrate injection mechanism 12 is integrated into the magnetic separation cleaning device 06 or the sample incubation device 05, so that the transfer path of the reaction container can be reduced, and the substrate injection mechanism 12 can be prevented from being arranged by redundant mechanisms, thereby reducing the cost and the occupied space.

Referring to fig. 1, optionally, the chemiluminescence analyzer further comprises a substrate bearing part 13, the substrate bearing part 13 is arranged on the bearing platform 11 and is positioned at the right edge of the bearing platform 11, the substrate bearing part 13 bears the substrate container, and the substrate in the substrate container is sucked by the substrate injection mechanism 12 and is conveyed into the reaction container. In this embodiment, the substrate carrying part 13 is located on the right side of the reagent loading device 02. The substrate holding portion 13 holds a substrate container, one end of the liquid path extends into the substrate container, and the other end of the liquid path is connected to the substrate injection mechanism 12 for transferring the substrate in the substrate container into the reaction container. The substrate holder 13 further includes a substrate pump, and the sample is transported by the substrate pump as a power source. Moreover, the substrate bearing part 13 is arranged on the front side of the bearing platform 11, so that after the substrate bearing part 13 is loaded with the substrate container, the substrate container can be close to a user, and the user can conveniently replace the substrate container. Preferably, the substrate carrier 13 can carry two substrate containers, so that either substrate container can be selected for substrate transport, facilitating substrate container replacement during operation of the apparatus.

As an implementation manner, the sample incubation device 05 includes an incubation block and a heating member disposed below the incubation block, the heating member is used for heating the incubation block, the incubation block is provided with a plurality of incubation holes arranged in an array, the incubation holes are used for placing a reaction container and performing incubation operation, and the incubation holes are also used for temporarily storing the reaction container after detection. The incubation block can bear the reaction container, the reaction container is placed in the incubation hole of the incubation block, and the incubation block performs incubation operation on the reaction container, so that the mixture in the reaction container can fully react to form a substance to be detected and impurities. The heating part can heat and incubate the piece, and it can bear reaction vessel to incubate the piece to heat the mixture in the reaction vessel, realize the function of incubating. The heating means can heat the mixture in the reaction vessel to a preset temperature, such as about 34 c, etc., prior to the actual measurement to ensure that the reaction is proceeding properly. Moreover, a plurality of holes of incubating can be arranged in arbitrary mode, and in this embodiment, a plurality of holes of incubating are arranged in array, can increase like this and incubate the piece and bear reaction vessel's quantity. Like this, when the piece of incubating satisfied the requirement of incubating, can also save occupation space, and then do benefit to the volume that reduces the complete machine. Of course, when the number of the incubation holes on the incubation block of the present invention is not enough, one incubation block with more incubation holes can be replaced to meet different use requirements of users. Illustratively, the incubation block is of a metal construction, which facilitates heat dissipation and thus heating of the reaction vessels in the incubation block. The heating component is a heating film which can generate heat after being electrified, and the heat can heat the incubation block. Of course, in other embodiments of the present invention, the heating member may also be a heating wire, a heating rod or other structures capable of heating. Compared with the structure of the conventional disc-shaped incubation disc, the sample incubation device 05 disclosed by the invention has the advantages that the structure is simpler, the design space is more compact, the calling is more flexible, and the periodical movement of the incubation disc is not limited.

The incubation hole is used for accommodating the incubation hole, and the incubation block is heated by the heating part so as to heat the reaction container in the incubation hole; meanwhile, the incubation hole in the incubation block can also accommodate the reaction container after photometry, and the purpose of temporarily storing the reaction container after photometry is achieved. The waste bin 15 is full or considered removed during the test, at which point the reaction vessel has just finished testing light and needs to be discarded. In order to ensure that the reaction container which has measured light does not occupy the position of the sample detection device 07 to affect the detection of the next sample, the transfer cup grasping device 08 transfers the reaction container which has measured light to the unused incubation hole of the sample incubation device 05 for temporary storage. After the waste bin 15 is emptied or returned, the transfer cup grasping device 08 discards the photometric reaction vessel temporarily stored in the sample incubation device 05 into the waste bin 15.

It can be understood that the mixture in the reaction container takes a certain time during incubation, and the transfer cup grasping device 08 can transfer the reaction container uniformly mixed by the mixing device 04 to the incubation hole of the incubation block, and since each component of the chemiluminescence analyzer of the present invention moves simultaneously, so that each position has the reaction container to operate, the transfer cup grasping device 08 can perform other operations without waiting for the completion of incubation of the reaction container, such as transferring the reaction container to the mixing device 04, transferring the reaction container from the magnetic separation cleaning device 06 to the mixing device 04 or the sample detection device 07, or transferring the incubated reaction container to the magnetic separation cleaning device 06.

Optionally, the sample incubation device 05 further comprises a temperature sensor disposed on the incubation block for detecting the temperature of the incubation block and controlling the heating temperature of the incubation block by the heating member. The temperature sensor is electrically connected with the main control device, the main control device can also detect the temperature of the incubation block through the temperature sensor, and also controls the heating part to heat the incubation block through the temperature sensor and adjusts the heating temperature of the incubation block heated by the heating part; specifically, the temperature sensor controls the output power of the heating part by detecting the temperature of the incubation block, so as to control the overall temperature of the incubation block. If the temperature sensor detects that the temperature of the incubation block is lower, the temperature sensor controls the heating part to heat so as to increase the temperature of the incubation block; if the temperature of the incubation block is higher, the temperature sensor controls the heating part to stop heating.

Still optionally, the sample incubation device 05 further includes a temperature switch, the temperature switch is disposed on the incubation block, and the temperature switch is used for controlling the heating component to stop heating. The temperature switch is electrically connected with the heating part and the main control device. When the temperature control function of the temperature sensor fails, the main control device controls the temperature switch to cut off the power supply of the heating part, so that high-temperature protection is realized, the sample in the reaction container is prevented from failing due to high temperature, and the accuracy of a sample detection result is ensured.

Referring to fig. 1 and fig. 6, as an embodiment, the chemiluminescence analyzer has a photometric well 09, the photometric well 09 is disposed independently of the sample incubation device 05 and is used for carrying a reaction container to be detected, and the sample detection device 07 is disposed at the photometric well 09 and is used for performing luminescence detection on the reaction container in the photometric well 09. That is, the photometric hole 09 is disposed on the platform 11 and located at the periphery of the sample incubation device 05, and the sample detection device 07 is disposed corresponding to the photometric hole 09 and also located at the periphery of the sample incubation device 05. Illustratively, the photometric well 09 and the sample detection device 07 are located on the back side of the incubation block of the sample incubation device 05. The transfer cup grabbing device 08 transfers the reaction container cleaned by the magnetic separation cleaning device 06 and then removed of impurities to the photometric hole 09, and the sample detection device 07 can perform photometry on an object to be detected in the reaction container in the photometric hole 09 to obtain a light intensity signal value so as to obtain various parameters of the sample.

Referring to fig. 5, of course, in other embodiments of the present invention, the photometric well 09 can also be located at the edge of the incubation block, and the sample detection device 07 is disposed at the side of the incubation block and corresponds to the photometric well 09. That is to say, photometry hole 09 sets up on hatching the piece, and correspondingly, sample detection device 07 sets up on hatching the piece to corresponding photometry hole 09, with the light intensity signal value of the determinand in the detection photometry hole 09 reaction vessel, be about to hatch function and detect the integrated setting of function, can make complete machine compact structure like this, reduce the volume, can also shorten reaction vessel's transfer path simultaneously, improve complete machine operating efficiency. Moreover, the sample detection device 07 is located on the rear side face of the sample incubation device 05 and is arranged side by side with the magnetic separation cleaning device 06, so that the occupied space can be reduced, the space utilization rate is improved, and the size of the whole machine is further reduced. Illustratively, the photometric well 09 is located at the edge of the incubation block to facilitate luminescence detection by the sample detection device 07.

Referring to fig. 1, the chemiluminescence analyzer also has a photometric opening, which communicates the photometric hole 09 with the sample detection device 07. During detection, the transfer cup grabbing device 08 transfers the reaction container to the photometric hole 09, the sample detection device 07 detects the luminous value of the object to be detected in the reaction container, and light emitted by the reaction container can irradiate the sample detection device 07 through the photometric opening, so that luminous detection of the object to be detected in the reaction container is realized. When the photometric hole 09 is arranged on the bearing platform 11, the photometric opening is located on the bearing platform 11; when the photometric well 09 is disposed on the incubation block, the photometric opening is correspondingly disposed on the incubation block.

It can be understood that if the substance to be detected does not need to be added with a substrate, the transfer cup grabbing device 08 directly transfers the reaction vessel after magnetic separation and cleaning from the magnetic separation and cleaning device 06 to the sample detection device 07 for luminescence detection. If the uniform mixing operation is not needed after the substrate is added to the object to be detected, the transfer cup grabbing device 08 directly transfers the reaction container after the magnetic separation cleaning from the magnetic separation cleaning device 06 to the sample incubation device 05, after the incubation block incubates the reaction container, the transfer cup grabbing device 08 transfers the reaction container from the incubation hole of the sample incubation device 05 to the photometric hole 09, and the sample detection device 07 performs luminescence detection on the reaction container in the photometric hole 09. If the substance to be detected needs to be mixed uniformly after being added with the substrate, the transfer cup grabbing device 08 transfers the reaction container after magnetic separation and cleaning from the magnetic separation cleaning device 06 to the mixing device 04 to uniformly mix the substance to be detected in the reaction container with the substrate, then the transfer cup grabbing device 08 transfers the reaction container from the mixing device 04 to the incubation hole of the incubation block, the reaction container is incubated by the incubation block, then the transfer cup grabbing device 08 transfers the reaction container from the incubation hole to the photometric hole 09, and the sample detection device 07 performs luminescence detection.

Further, in order to ensure that the reaction container in the photometric hole 09 is not affected by ambient light during the photometric process, the chemiluminescent analyzer further includes a switch door, a cover that can be opened and closed is disposed on the photometric hole 09, and when the sample detection device 07 performs the luminescence detection on the reaction container in the photometric hole 09, the switch door closes the photometric hole 09. When the sample detection device 07 does not perform luminescence detection, the opening and closing door is in an open state; when the transfer cup grabbing device 08 transfers the reaction container to the photometric hole 09, the door is opened and closed to close the photometric hole 09, so that the reaction container in the photometric hole 09 is shielded, and the accuracy of detecting a sample by the sample detection device 07 is improved; after the light is measured, the switch door is opened, and the transfer cup grabbing device 08 discards the reaction container of the light measuring hole 09.

Referring to fig. 1 and 5, as an implementation manner, the number of the blending devices 04 is at least two, at least two blending devices 04 are arranged side by side and are driven independently, and when one blending device 04 performs operations of adding a sample and a reagent, the other blending devices 04 can drive the reaction container to perform blending operations. At least two mixing devices 04 set up each other independently, and mutual independent operation, like this, two at least mixing devices 04 can bear two at least reaction vessel for the chemiluminescence analysis appearance can reduce reaction vessel's latency to two at least mixing devices 04 executive operation, improves chemiluminescence analysis appearance's functioning speed. Specifically, the transfer cup grabbing device 08 transfers the reaction containers to at least two mixing devices 04 respectively, when a sample and a reagent are added to the reaction container in one mixing device 04, the rest mixing containers can perform mixing operation on the reaction container added with the sample and the reagent, so that the sample adding process and the mixing process are performed simultaneously, the processing speed of the sample is increased, and the work efficiency and the test flux of the whole machine are further improved.

In this embodiment, the blending device 04 includes a first blending device 041 and a second blending device 042, and the first blending device 041 and the second blending device 042 are arranged side by side and located at the right side of the sample incubation device 05. After the first blending device 041 and the second blending device 042 are adopted, the chemiluminescence analyzer of the invention provides a working mode in which the two blending mechanisms alternately operate, so as to improve the working efficiency and the test flux of the chemiluminescence analyzer. Specifically, when the first mixer 041 performs the sample and reagent adding operation, the second mixer 042 can perform the mixing operation on the reaction container to which the sample and the reagent have been added. That is, when the dispensing device 03 performs the operation of adding the sample and the reagent in the first blending device 041 in the period, the second blending device 042 may perform the operation of blending the mixture after the sample and the reagent are added in the previous period, and after the mixture is blended, the cup grabbing device 08 is transferred to transfer the reaction container to the sample incubation device 05; in the next cycle, the first mixer 041 performs a mixing operation, and the second mixer 042 performs a dispensing operation for a new sample and reagent to be tested. The chemiluminescence analyzer adopts the first blending device 041 and the second blending device 042, and the reaction container which is required to be emptied originally in one period is transferred to the blending device 04, and the sample and the reagent are injected, mixed and blended, and then transferred to the sample incubation device 05, so that the actions are alternately completed through two blending loads in two periods, which is equivalent to shortening the time of one period and improving the working efficiency and the testing flux of the analyzer.

It can be understood that, after the chemiluminescence analyzer of the present invention adopts two blending devices 04, when one of the blending devices 04 fails, the other blending device 04 can still operate to perform blending operation, so that the whole machine can still be used. At this moment, the chemiluminescence analyzer adopts the working mode of operation of a single blending device 04, and the test beat can be correspondingly adjusted in other decorations to work in cooperation with the single blending device 04. In other embodiments, the number of the blending devices 04 may be more than 2, and is not described herein again.

Optionally, the chemiluminescence analyzer of the present invention employs two blending devices 04, which not only can complete the functions of separate injection and blending of a sample and a reagent, but also can complete the function of pre-dilution of a sample in two cycles, that is, in this cycle, after the separate injection device 03 performs separate injection operation of a sample and a reagent in the first blending device 041, the operation of uniformly mixing a mixture is completed in this cycle, in the next cycle, the cup grabbing device is transferred to grab an empty reaction container and place the empty reaction container on the second blending device 042, after the separate injection device 03 sucks the uniformly mixed mixture in the first blending device 041, the mixture moves to the second blending device 042 to be discharged into liquid, and then the separate injection device 03 separately injects a corresponding reagent, thereby completing the function of pre-dilution of a sample. Thus, the detection range of the chemiluminescence analyzer of the present invention can be widened to obtain a wider range of detection parameters.

Referring to fig. 6, of course, in another embodiment of the present invention, when the chemiluminescence analyzer performs a kneading operation using one kneading apparatus 04, the chemiluminescence analyzer may also perform a fixed dilution operation to widen a detection range. Specifically, the chemiluminescence analyzer further comprises a fixed dilution hole 16, the fixed dilution hole 16 is arranged adjacent to the mixing device 04, the fixed dilution hole 16 is used for bearing a reaction container to be diluted, a dispensing device 03 transfers a mixture in the reaction container in the fixed dilution hole 16 to the reaction container of the mixing device 04, and the dispensing device 03 transfers a reagent to dilute a sample. Alternatively, the fixed dilution holes 16 may be provided on the incubation block, but of course, the fixed dilution holes 16 may also be provided on the carrier platform 11 independently of the incubation block. The fixed dilution holes 16 are provided at the right edge of the incubation block to shorten the path for the dispensing device 03 to transfer the mixture and the reagent, thereby improving the efficiency. After the blending device 04 performs sample and reagent separate injection operation, the operation of mixing the mixture is completed, then the cup grabbing device is transferred to grab an empty reaction container and place the empty reaction container into the fixed dilution hole 16, the separate injection device 03 sucks the mixed mixture from the blending device 04, the mixed mixture moves to the fixed dilution hole 16 and is discharged into liquid, and then the separate injection device 03 separately injects a corresponding reagent, so that the function of pre-diluting the sample is completed. Thus, the detection range of the chemiluminescence analyzer of the present invention can be widened to obtain a wider range of detection parameters.

Referring to fig. 1, as an implementation manner, the dispensing device 03 includes a dispensing driving mechanism and a dispensing needle that move in three directions of XYZ, and the dispensing needle is mounted on the dispensing driving mechanism, so that the dispensing driving mechanism can drive the dispensing needle to move in three directions of XYZ, thereby realizing movement at any position in a three-dimensional space, and realizing transfer of a sample and a reagent. Specifically, the dispensing driving mechanism can drive the dispensing needle to move to the sample loading device 01 to suck a sample, move to the reagent loading device 02 to suck a reagent, move to the blending device 04 to add a sample or a reagent, add a reagent into the fixed dilution hole 16 of the incubation block, and the like, so that the transfer operation of the sample and the reagent is realized. The separate injection driving mechanism can adopt a synchronous belt structure which is matched with a motor to move in the three directions of XYZ, and the synchronous belt structure can be replaced by a chain transmission structure, a gear rack structure or other structures capable of realizing linear motion. When a sample or a reagent is sucked, the separate injection driving mechanism drives the separate injection needle to descend, and after the suction is finished, the separate injection driving mechanism drives the separate injection needle to ascend; then, the separate injection driving mechanism drives a separate injection needle to move to a mixing device 04 in a horizontal plane; the dispensing driving mechanism drives the dispensing needle to descend, the dispensing needle discharges samples or reagents, and after the discharging is finished, the dispensing driving mechanism drives the dispensing needle to ascend; the dispensing drive mechanism returns the dispensing needle to the initial position or performs the sample or reagent transfer operation again.

It can be understood that the dispensing needle needs to be cleaned after each sample and reagent is sucked or discharged, so as to avoid the sample or reagent remaining on the outer wall of the dispensing needle to cause cross contamination and affect the accuracy of sample detection. Therefore, the chemiluminescence analyzer further comprises a cleaning pool 10, the cleaning pool 10 is positioned on one side of the homogenizing device 04 away from the incubation block, the cleaning pool 10 is also positioned on the rear side of the sample loading device 01, and the cleaning pool 10 is used for cleaning the dispensing device 03. Specifically, the dispensing driving mechanism drives the dispensing needle to move to the cleaning pool 10, the dispensing driving mechanism drives the dispensing needle to descend, the dispensing needle extends into the cleaning pool 10, and the inner wall and the outer wall of the dispensing needle are cleaned by the cleaning liquid in the cleaning pool 10; after the completion of the cleaning, the dispensing drive mechanism drives the dispensing needle to ascend, and then performs other operations. It will be appreciated that the wash tank 10 is connected to a fluid path for delivering wash fluid and for removing waste fluid from the wash. Of course, in another embodiment of the present invention, the liquid path may be connected to the dispensing needle, and the inner wall of the dispensing needle may be cleaned by feeding the cleaning liquid into the dispensing needle, and when the outer wall is cleaned by the cleaning cell 10, the waste cleaning liquid in the dispensing needle may be discharged through the cleaning cell 10.

As an embodiment, the sample loading device 01 includes a plurality of sample racks arranged side by side, each sample rack carrying a plurality of sample containers. It will be appreciated that a plurality of sample racks are arranged side by side on the carrier platform 11 and the dispensing device 03 can be moved to the sample loading device 01 to aspirate samples from sample containers at various locations. Of course, in other embodiments of the present invention, the sample rack may be transferred to a designated position by a sample transfer mechanism, such as a gear transmission mechanism, a chain transmission mechanism, or the like, and the dispensing device 03 may aspirate a sample at the designated position.

Further, the right side of the loading platform 11 has an area where the specimen loading device 01 is placed, and a plurality of specimen racks are placed side by side in the area, and as long as the area can be placed, the specimen racks can be placed. Therefore, the sample loading device 01 of the present invention has high expandability, and the user can increase or decrease the number of sample racks according to the actual use requirement. In this embodiment, the number of the sample racks is six, and of course, the user can increase or decrease the sample racks according to the actual use requirement.

Referring to fig. 1, as an embodiment, the reagent loading device 02 includes a plurality of reagent cartridges 021 arranged in an array, each reagent cartridge 021 being adapted to carry a plurality of reagent containers. The plurality of reagent boxes 021 arranged in an array form can increase the number of reagent containers, and the reagent containers can be placed as many as possible in a limited range so as to meet different detection requirements of samples. It is understood that the reagent cartridges 021 may be arranged in at least two rows. Each reagent kit 021 carries a plurality of reagent containers arranged in a row, and it is understood that the reagent kit 021 may have a box-like structure, and the reagent containers may be directly placed in the reagent kit 021, and of course, the reagent kit 021 may also be used as reagent containers, and the inside of the reagent kit 021 is divided into a plurality of spaces, each of which is a reagent container and in which one type of reagent may be placed; it can also be a tray structure, the reagent container is placed on the reagent box 021; of course, a combination of both configurations is also possible.

It can be understood that, when the chemiluminescence analyzer of the present invention uses the magnetic particle method to detect a sample, the reagent loading device 02 needs to hold a magnetic particle reagent, but the magnetic particles in the magnetic particle reagent will precipitate on the substrate of the reagent container after being immobilized for a long time, and the dispensing device 03 cannot suck the magnetic particles when the reagent cannot be sucked, which affects the detection result. Thus, the reagent loading device 02 can also mix the magnetic particle reagent in the reagent vessel. Of course, when the chemiluminescence analyzer does not perform magnetic particle detection, the reagent loading device 02 may not mix the reagents in the reagent containers.

Specifically, the reagent loading device 02 further comprises a reagent storage transmission mechanism 022 and a reagent storage driving mechanism, wherein a plurality of reagent cartridges 021 are arranged in two rows and respectively located at two sides of the reagent storage transmission mechanism 022, and each reagent cartridge 021 has a mixing position and a rest position for respectively carrying a reaction container; the reagent storage transmission mechanism 022 is in transmission connection with the reagent storage driving mechanism and the plurality of reagent kits 021, and drives the reagent containers on the mixing positions of the reagent kits 021 to rotate so as to mix the reagents in the reagent containers uniformly. It can be understood that kit 021 has a plurality of static positions and a mixing position, and a plurality of static positions set up side by side with the mixing position, and the mixing position is located kit 021's border position, and the reagent container of treating the mixing is placed on the mixing position, and moreover, a plurality of reagent positions are two lines and about reagent storage drive mechanism 022 symmetry setting, and reagent storage drive mechanism 022 corresponds the mixing position respectively. When the reagent is required to be uniformly mixed, the reagent storage driving mechanism 022 drives the reagent container in the uniformly mixing position to rotate, so that the magnetic particle reagent in the reagent container is uniformly mixed.

Further, reagent storage drive mechanism 022 includes reagent storage drive structure, mixing part 0222 and a plurality of mixing gear chassis 0221 that have tooth portion, reagent storage drive structure is connected with mixing part 0222, mixing gear chassis 0221 is located the mixing position of kit 021, and bear the weight of the reagent container, mixing part 0222 is connected through a plurality of mixing gear chassis 0221 transmissions of tooth portion with both sides, reagent storage drive structure drives mixing gear chassis 0221 through mixing part 0222 and rotates, with the reagent in the mixing reagent container. Referring to fig. 3, that is, the number of the kneading members 0222 is one, and one kneading member 0222 is engaged with the kneading gear chassis 0221 on both sides. Reagent storage drive mechanism connects mixing part 0222 and reagent storage actuating mechanism, and reagent storage actuating mechanism drives mixing part 0222 through reagent drive structure and moves to drive mixing gear chassis 0221 and rotate, move with the reagent container that drives on mixing gear chassis 0221, realize the magnetic particle reagent mixing in the mixing reagent container.

Referring to fig. 1 and 2, of course, the number of the kneading members 0222 having teeth may be two, and the two kneading members 0222 are symmetrically disposed, and the teeth are respectively disposed toward the reagent cartridge 021 and engaged with the kneading gear chassis 0221 in the reagent cartridge 021. Two mixing parts 0222 are connected with reagent storage drive structure respectively, and mixing gear chassis 0221 is located the mixing position of kit 021 to bear the weight of the reagent container, two mixing parts 0222 are connected through tooth portion and the transmission of a plurality of mixing gear chassis 0221 that correspond respectively, and reagent storage drive structure drives mixing gear chassis 0221 through mixing parts 0222 and rotates, with the magnetic reagent in the mixing reagent container.

It can be understood that the kneading member 0222 with the tooth portion is a rack structure in this embodiment, and the kneading member 0222 drives the kneading gear base 0221 to rotate. Mixing part 0222 is linear motion promptly, and reagent storage actuating mechanism drives reagent storage drive structure and is linear motion, can be cylinder structure, gear cooperation motor structure etc..

Referring to fig. 1 and 4, the chemiluminescence analyzer of the present invention employs a reagent loading device 02 having a symmetric arrangement of magnetic particles and a uniform mixing function to expand the number of reagent containers, so that the structure is more compact and the design space is saved. Optionally, the reagent cartridge 021 extends in a direction parallel or perpendicular to the direction in which the sample loading device 01 stores the sample containers. That is, the reagent loading device 02 can be placed horizontally or vertically, and can meet the requirement of reagent suction.

Referring to fig. 1, as an embodiment, the magnetic separation cleaning apparatus 06 includes a magnetic separation base, a cleaning solution injection mechanism, a cleaning solution discharge mechanism, and a magnetic separation adsorption mechanism. The magnetic separation bottom plays a bearing role and is used for bearing all parts of the magnetic separation cleaning device 06, and meanwhile, the magnetic separation base can also bear a reaction vessel to be cleaned. The transfer cup grabbing device 08 transfers the reaction vessel from the sample incubation device 05 to the magnetic separation base, and after the reaction vessel is cleaned by the magnetic separation cleaning device 06, the transfer cup grabbing device 08 transfers the reaction vessel from the magnetic separation base. If the substrate is added, after the substrate is added to the magnetic separation base, the transfer cup grasping device 08 transfers the reaction vessel from the magnetic separation base. The cleaning liquid injection mechanism is connected with the liquid path and can inject cleaning liquid into the reaction vessel of the magnetic separation base, and the cleaning liquid discharge mechanism is connected with the liquid path and can discharge cleaning waste liquid and the cleaned cleaning liquid in the reaction vessel of the magnetic separation base. It is to be understood that the step of injecting the cleaning liquid by the cleaning liquid injecting mechanism is performed before the cleaning liquid is discharged by the cleaning liquid discharging mechanism. The cleaning liquid injection mechanism and the cleaning liquid discharge mechanism are used in a pair.

As an example, the number of the cleaning liquid injection mechanism and the cleaning liquid discharge mechanism is plural, that is, the magnetic separation cleaning apparatus 06 has a multistage magnetic separation cleaning function. In the present embodiment, the number of the cleaning liquid injection mechanisms and the cleaning liquid discharge mechanisms is three. The three cleaning fluid injection mechanisms and the three cleaning fluid discharge mechanisms are distributed on the magnetic separation base in a staggered mode, namely the cleaning fluid injection mechanisms are arranged on two sides of each cleaning fluid discharge mechanism respectively, and the cleaning fluid discharge mechanisms are arranged on two sides of each cleaning fluid injection mechanism respectively. It can be understood that the fluid path is a pipeline and is matched with a pump and the like. Illustratively, the cleaning liquid injection mechanism comprises a liquid injection needle and a liquid injection needle seat, wherein the liquid injection needle seat is fixed in the cleaning liquid inlet hole, and the liquid injection needle is used for adding the cleaning liquid into the reaction container. The cleaning liquid discharge mechanism comprises a liquid discharge needle and a liquid discharge pipeline, and the cleaning liquid waste in the reaction container is sucked by the liquid discharge needle and is discharged by the liquid discharge pipeline.

After the transfer cup grabbing device 08 grabs the reaction vessel to the magnetic separation cleaning device 06, the magnetic separation cleaning device 06 advances periodically, multi-magnetic separation cleaning of the reaction vessel is completed through the plurality of cleaning solution injection mechanisms and the cleaning solution discharge mechanism, after cleaning is completed, the substrate is injected into the reaction vessel through the substrate injection mechanism 12, and after injection is completed, the transfer cup grabbing device 08 grabs the reaction vessel out.

The magnetic separation adsorption mechanism is arranged in the magnetic separation base and is positioned at two sides of the rotating path of the reaction vessel. The magnetic separation adsorption mechanism can adsorb magnetic beads in the reaction container to the side wall of the reaction container, so that the objects to be detected and impurities in the reaction container are cleaned. It can be understood that the object to be detected and the impurities in the reaction vessel are cleaned through at least one separation and cleaning operation, so that the purity of the object to be detected is ensured, and the accuracy of a sample detection result is further ensured. In this embodiment, the magnetic separation and adsorption mechanism is a magnet.

The invention also provides an analysis method of the chemiluminescence analyzer, which comprises the following steps:

a step of adding a cup, in which the transferring and cup grabbing device 08 transfers the empty reaction container to the first blending device 041 and the second blending device 042;

in this sample adding step, the dispensing device 03 transfers the sample in the sample loading device 01 to the reaction container of the first mixing device 041;

a reagent adding step of transferring the reagent in the reagent storage device to the reaction container of the first mixer 041 by the dispensing device 03;

a blending step, in which the first blending device 041 uniformly mixes the sample and the reagent in the reaction container; meanwhile, the sample adding step and the reagent adding step are executed on the second blending device 042;

an incubation step, in which the transfer cup grasping device 08 transfers the uniformly mixed reaction container to the sample incubation device 05, and the sample incubation device 05 incubates the uniformly mixed mixture in the reaction container;

a magnetic separation cleaning step, namely transferring the incubated reaction container to a magnetic separation cleaning device 06 by using a transfer cup grabbing device 08, and removing impurities in the incubated reaction container by using the magnetic separation cleaning device 06;

and a detection step, namely transferring the cleaned reaction container to a sample detection device 07 by using a transfer cup grabbing device 08, and performing luminescence detection on the object to be detected in the cleaned reaction container by using the sample detection device 07.

When a reagent adding operation is performed once, i.e. a one-step reaction workflow, a user manually loads two reaction containers in the reaction container loading device 14, places the two reaction containers on the two reaction container loading devices 14, respectively, grabs a new reaction container on the reaction container loading device 14 by the transfer cup grabbing device 08, and places the new reaction container on the first blending device 041. The dispensing device 03 sucks the sample in the sample loading device 01, and transfers the sample to the reaction container on the first mixer 041; the dispensing device 03 further moves to the reagent loading device 02 to suck the required reagent and then transfers the reagent to the reaction container on the first mixing device 041; it should be noted that, every time the dispensing device 03 sucks a component, the dispensing device 03 moves to the cleaning cell 10 to perform outer wall cleaning. After the sample and the reagent are added, the first mixing device 041 performs mixing of the mixture for a fixed time, so that the sample and the reagent are sufficiently mixed.

In order to improve the working efficiency of the whole machine, in the process of performing mixture blending, the first blending device 041 transfers the cup grasping device 08 to grasp an empty reaction container to the second blending device 042, and the separate injection device 03 completes addition of a sample and a reagent on the second blending device 042. The first mixer 041 and the second mixer 042 operate alternately. After the first blending device 041 finishes blending the mixture, the transfer cup grabbing device 08 transfers the blended reaction container to the incubation hole of the sample incubation device 05 for incubation for a period of time, and the subsequent reaction containers finished blending are sequentially placed in the incubation holes of the sample incubation device.

After the preset incubation time is reached, the transfer cup grabbing device 08 grabs the reaction container into the magnetic separation cleaning device 06, then in a plurality of cycles, the mixture in the reaction container is cleaned, the cleaning is completed, the substrate injection mechanism 12 injects the substrate into the cleaned reaction container, the substrate injected reaction container is transferred into the incubation hole on the sample transfer device by the transfer cup grabbing device 08, the substrate incubation is performed for a fixed time, after the substrate incubation is completed, the switch door on the photometric hole 09 is opened, the transfer cup grabbing device 08 transfers the reaction container into the photometric hole 09, then the switch door on the photometric hole 09 is closed, and the sample detection device 07 starts to perform the photometric operation for a period of time.

After the sample detection device 07 finishes the photometric operation, the switch door on the photometric hole 09 is opened again, and the transfer cup grasping device 08 grasps the reaction vessel above the waste bin 15 and discards it.

As an embodiment, the analysis method further comprises the steps of:

after the homogenization step or after the magnetic separation washing step, at least one more reagent addition step is performed.

That is, the sample is subjected to a plurality of reagent adding operations, and the reaction vessel is subjected to at least one reagent adding operation after mixing and before incubation or after incubation and after washing, so that the sample can be sufficiently reacted. In this embodiment, the reagent adding operation is performed once on the reaction vessel after the mixing and before the incubation or after the incubation and after the cleaning, that is, the reagent adding operation is performed twice in total, which is a two-step reaction workflow.

Specifically, the transfer cup grasping device 08 transfers the reaction container which completes the incubation of the mixture from the incubation hole of the sample incubation device 05 to the mixing device 04 or transfers the reaction container which completes the magnetic separation, cleaning and separation from the magnetic separation cleaning device 06 to the mixing device 04, and then the dispensing device 03 moves to the reagent container in the reagent loading device 02 to suck the reagent, and the reagent container is added to the reaction container of the mixing device 04, so that the operation of adding the reagent in the second step is completed. The follow-up action is the same as the one-step reaction workflow.

As an embodiment, the analysis method further comprises the steps of:

The chemiluminescence analyzer can also perform dilution operation to widen the detection range of the chemiluminescence analyzer and obtain wider detection parameters.

Further, the diluting step comprises the steps of:

the transfer cup grabbing device 08 transfers the empty reaction container to the second blending device 042;

the dispensing device 03 transfers the mixture portion in the reaction container in the first kneading device 041 to the reaction container in the second kneading device 042;

the reagent addition step is performed on the reaction vessel in the second homomixer 042.

In this period, after the first blending device 041 performs sample and reagent separate injection operation, the separate injection device 03 completes mixture mixing operation in this period, in the next period, the cup grabbing device is transferred to grab an empty reaction container and place the empty reaction container on the second blending device 042, after the first blending device 041 sucks the mixed mixture, the separate injection device 03 moves to the second blending device 042 to discharge the mixed mixture into liquid, and then the separate injection device 03 separately injects a corresponding reagent to complete the sample pre-dilution function. So as to widen the detection range of the chemiluminescence analyzer and obtain wider detection parameters.

As an embodiment, before and after the step of adding the reagent, the analysis method further comprises a washing step, and the dispensing device 03 moves to the washing tank 10 for washing.

That is, the dispensing needle of the dispensing device 03 needs to be cleaned after sucking or discharging the sample and the reagent, so as to avoid the influence of cross contamination caused by the sample or the reagent remaining on the outer wall of the dispensing needle on the accuracy of sample detection.

In one embodiment, the method further comprises a reagent mixing step for mixing the magnetic particle reagent in the reagent container before the reagent adding step when the reagent container is loaded with the magnetic particle reagent.

When the chemiluminescence analyzer adopts a magnetic particle method to detect a sample, a magnetic particle reagent needs to be placed in the reagent loading device 02, and before the dispensing device 03 sucks the reagent, the reagent loading device 02 mixes the magnetic particle reagent in the reagent container, so that the magnetic particle reagent is not deposited at the bottom and is uniformly mixed, and the dispensing device 03 can be ensured to suck the magnetic particles. Of course, when the chemiluminescence analyzer does not perform magnetic particle detection, the reagent loading device 02 may not mix the reagents in the reagent containers.

The technical features of the embodiments described above can be arbitrarily combined, and for the sake of brevity, all possible combinations of the technical features in the embodiments described above are not described, but should be considered as the scope of the present specification as long as there is no contradiction between the combinations of the technical features.

The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the present invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.

Claims

A chemiluminescence analyzer is characterized by comprising a sample loading device for storing a sample, a reagent loading device for storing a reagent, a separate injection device for sucking and discharging the sample and the reagent, a uniform mixing device for supporting a reaction container, a sample incubation device for incubation, a magnetic separation cleaning device for separation cleaning, a sample detection device for luminescence detection and a transfer cup grabbing device for transferring the reaction container;

the sample loading device and the reagent loading device are arranged side by side, the sample incubation device is respectively arranged adjacent to the blending device, the magnetic separation cleaning device and the sample detection device, the sample incubation device, the blending device, the magnetic separation cleaning device and the sample detection device are positioned on the same side of the sample loading device, and the transfer cup grabbing device can move to the positions above the blending device, the sample incubation device, the magnetic separation device and the sample detection device;

the transferring cup grabbing device transfers the reaction container to the blending device; the dispensing device can move above the sample loading device and the reagent loading device and can respectively transfer the sample and the reagent into a reaction container of the blending device; the transfer grab the glass device will reaction vessel follows mixing device transfers to incubate in the device is hatched to the sample, transfer grab the glass device still will incubate after reaction vessel transfers to magnetic separation belt cleaning device separates the washing, and will separate after the washing reaction vessel transfer to it carries out luminescence detection to incubate among the photometric device.
The chemiluminescent analyzer of claim 1 further comprising a carrying platform, wherein the sample loading device and the reagent loading device are located side by side on the right side of the carrying platform, the magnetic separation cleaning device, the sample detection device, the sample incubation device and the blending device are located on the left rear side of the carrying platform, the blending device is located on the right side of the sample incubation device, the dispensing device is located on the right rear side of the carrying platform, and the transfer cup grasping device is located on the left front side of the carrying platform.
The chemiluminescence analyzer according to claim 1 or 2, wherein the number of the mixing devices is at least two, at least two mixing devices are arranged side by side and are driven independently, and when one mixing device is used for adding a sample and a reagent, the other mixing devices can drive the reaction container to perform mixing operation.
The chemiluminescence analyzer according to claim 1 or 2, wherein the sample incubation device comprises an incubation block and a heating member disposed below the incubation block, the heating member is used for heating the incubation block, the incubation block is provided with a plurality of incubation holes arranged in an array, the incubation holes are used for placing the reaction containers and performing incubation operation, and the incubation holes are also used for temporarily storing the reaction containers after detection.
The chemiluminescence analyzer according to claim 1, wherein the chemiluminescence analyzer has a photometric well which is provided independently of the sample incubation device and is used for carrying the reaction vessel to be detected, and the sample detection device is provided at the photometric well and is used for luminescence detection of the reaction vessel in the photometric well.
The chemiluminescence analyzer according to claim 1, wherein the chemiluminescence analyzer has a photometric hole, the sample incubation device comprises an incubation block, the photometric hole is located at an edge position of the incubation block, and the sample detection device is disposed at a side surface of the incubation block and corresponds to the photometric hole.
The chemiluminescence analyzer according to claim 5 or 6, further comprising a switch door, wherein the switch door is capable of opening and closing the cover and is disposed on the photometric well, and the switch door closes the photometric well when the sample detection device detects luminescence of the reaction vessel in the photometric well.
The chemiluminescence analyzer according to claim 1 or 2, wherein the sample incubation device comprises an incubation block, the incubation block is provided with a fixed dilution hole, the fixed dilution hole is arranged adjacent to the mixing device, the fixed dilution hole is used for carrying the reaction vessel to be diluted, the mixture in the reaction vessel in the fixed dilution hole is transferred to the reaction vessel of the mixing device by the dispensing device, and the reagent is transferred by the dispensing device to dilute the sample.
The chemiluminescence analyzer according to claim 1 or 2, wherein the mixing device and the reagent loading device are respectively provided at both sides of the sample loading device, or the mixing device and the sample loading device are respectively provided at both sides of the reagent loading device;

the reagent loading device comprises a plurality of reagent boxes arranged in an array, and each reagent box is used for bearing a plurality of reagent containers.
The chemiluminescence analyzer according to claim 9, wherein the reagent loading device further comprises a reagent storage transmission mechanism and a reagent storage driving mechanism, wherein a plurality of reagent cartridges are arranged in two rows and respectively located at two sides of the reagent storage transmission mechanism, each reagent cartridge has a mixing position and a rest position for respectively carrying the reaction vessels; the reagent storage transmission mechanism is in transmission connection with the reagent storage driving mechanism and the plurality of reagent boxes and drives the reagent containers on the mixing positions of the reagent boxes to rotate so as to mix the reagents in the reagent containers.
The chemiluminescence analyzer according to claim 10, wherein the reagent storage drive mechanism comprises a reagent storage drive structure, a blending part with a tooth part and a plurality of blending gear chassis, the reagent storage drive structure is connected with the blending part, the blending gear chassis is positioned on the blending position of the reagent kit and bears the reagent container, the blending part is in drive connection with the plurality of blending gear chassis on two sides through the tooth part, and the reagent storage drive structure drives the blending gear chassis to rotate through the blending part so as to blend the reagent in the reagent container.
The chemiluminescence analyzer according to claim 10, wherein the reagent storage transmission mechanism comprises a reagent storage transmission structure, two blending parts with tooth parts and a plurality of blending gear chassis, the two blending parts are respectively connected with the reagent storage transmission structure, the blending gear chassis is positioned on the blending position of the reagent kit and bears the reagent container, the two blending parts are respectively in transmission connection with the corresponding blending gear chassis through the tooth parts, and the reagent storage transmission structure drives the blending gear chassis to rotate through the blending parts so as to blend the magnetic reagent in the reagent container.
A chemiluminescent analyzer according to claim 1 wherein the sample loading means comprises a plurality of sample racks arranged side by side, each sample rack carrying a plurality of sample containers.
The chemiluminescence analyzer of claim 1, further comprising a bearing platform and a cleaning pool, wherein the cleaning pool is located on one side of the blending device away from the incubation block, the cleaning pool is located on the rear side of the sample loading device, and the cleaning pool is used for cleaning the dispensing device.
The chemiluminescent analyzer of claim 1 further comprising a substrate injection mechanism for adding a substrate into the reaction vessel;

the substrate injection mechanism is arranged on the magnetic separation cleaning device, or the substrate injection mechanism is arranged on the sample incubation device.
The chemiluminescent analyzer of claim 1 further comprising a carrying platform, a substrate carrying part and a substrate injecting mechanism, wherein the substrate carrying part is arranged on the carrying platform and is positioned at the right side edge of the carrying platform, the substrate carrying part carries a substrate container, and the substrate injecting mechanism sucks the substrate in the substrate container and conveys the substrate into the reaction container.
The chemiluminescent analyzer of claim 2 further comprising a reaction vessel loading device disposed on the carrying platform and located at the front side of the sample incubation device for carrying the reaction vessel; the transferring cup grabbing device can move to the position above the reaction container loading device to grab the reaction container in the reaction container loading device and transfer the reaction container to the blending device.
The chemiluminescent analyzer of claim 17 further comprising a waste bin disposed on the carrier platform and located to the right of the reaction vessel loading device for recycling the reaction vessels after detection; the transfer cup grasping device is movable to above the waste bin to grasp the reaction vessel at the sample incubating device and transfer to the waste bin.
An analysis method of a chemiluminescence analyzer is characterized in that the chemiluminescence analyzer comprises a sample loading device, a reagent loading device, a separate injection device, a mixing device, a sample incubation device, a magnetic separation cleaning device, a sample detection device and a transfer cup grabbing device, wherein the mixing device comprises a first mixing device and a second mixing device; the analysis method comprises the following steps:

a step of adding the cup, in which the transferring cup grabbing device transfers the empty reaction container to the first blending device and the second blending device;

adding a sample, wherein the separate injection device transfers the sample in the sample loading device to a reaction container of the first mixing device;

a reagent adding step of transferring the reagent in the reagent storage device to a reaction vessel of the first mixing device by the dispensing device;

uniformly mixing, namely uniformly mixing the sample and the reagent in the reaction container by using the first uniformly mixing device; simultaneously, executing the sample adding step and the reagent adding step on the second blending device;

an incubation step, wherein the transfer cup grabbing device transfers the uniformly mixed reaction container to a sample incubation device, and the sample incubation device incubates the uniformly mixed mixture in the reaction container;

a magnetic separation cleaning step, wherein the incubated reaction vessel is transferred to a magnetic separation cleaning device by the transfer cup grabbing device, and impurities in the incubated reaction vessel are removed by the magnetic separation cleaning device;

and a detection step, wherein the cleaned reaction container is transferred to the sample detection device by the transfer cup grabbing device, and the sample detection device performs luminescence detection on the cleaned object to be detected in the reaction container.
The analytical method of a chemiluminescent analyzer of claim 19 further comprising the steps of:

and after the uniformly mixing step or after the magnetic separation and cleaning step, performing the reagent adding step at least once again.
The analytical method of a chemiluminescent analyzer of claim 19 further comprising the steps of:

prior to the incubation step, a dilution step is performed, the dilution step being used to dilute the sample.
The analytical method of a chemiluminescent analyzer of claim 21 wherein the diluting step comprises the steps of:

the transferring cup grabbing device transfers the empty reaction container to the second blending device;

the dispensing device transfers a mixture portion in the reaction vessel in the first kneading device to the reaction vessel in the second kneading device;

and (c) performing the reagent adding step on the reaction vessel in the second blending device.
The analytical method of a chemiluminescent analyzer of claim 21 further comprising a cleaning bath; before and after the sample adding step and before and after the reagent adding step, the analysis method further comprises a washing step, and the dispensing device moves to the washing pool for washing.
The analytical method for a chemiluminescent analyzer of claim 19 further comprising a reagent blending step for blending the magnetic particle reagent in the reagent container before the reagent adding step when the reagent container is loaded with the magnetic particle reagent.