CN107384776A - Micro-fluidic chip - Google Patents
Micro-fluidic chip Download PDFInfo
- Publication number
- CN107384776A CN107384776A CN201710661755.XA CN201710661755A CN107384776A CN 107384776 A CN107384776 A CN 107384776A CN 201710661755 A CN201710661755 A CN 201710661755A CN 107384776 A CN107384776 A CN 107384776A
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- CN
- China
- Prior art keywords
- waste liquid
- micro
- entrance
- fluidic chip
- reative cell
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
Abstract
The invention provides a kind of micro-fluidic chip, including thin-film carrier, the thin-film carrier is provided with mixing chamber, magnetically attractive reative cell, constant-temperature amplification room, observation ward and waste liquid pool, the entrance of the mixing chamber is connected with adding mouth and air inlet, the outlet of the mixing chamber is connected with the entrance of the magnetically attractive reative cell, the outlet of the magnetically attractive reative cell is connected with the entrance of the constant-temperature amplification room, the outlet of the constant-temperature amplification room is connected with the entrance of the observation ward, and the outlet of the observation ward is connected with the entrance of the waste liquid pool.The beneficial effects of the invention are as follows：With integrated level height, small volume, operate the characteristics of simple, cost is low, applicability is wide.
Description
Technical field
The present invention relates to test device, more particularly to a kind of micro-fluidic chip.
Background technology
The detection majority such as traditional PCR tests is completed in laboratory, and Primary containers are test tubes, mixing, heating, is inhaled
Magnetic, reaction, detection etc. are all in vitro to complete, and are displaced through pipette to reach.Complete to detect by laboratory, cost
Higher, applicability is smaller.
The content of the invention
In order to solve the problems of the prior art, the invention provides a kind of very wide micro-fluidic chip of applicability.
The invention provides a kind of micro-fluidic chip, including thin-film carrier, the thin-film carrier is provided with mixing chamber, magnetically attractive
Reative cell, constant-temperature amplification room, observation ward and waste liquid pool, the entrance of the mixing chamber is connected with adding mouth and air inlet, described mixed
The outlet for closing room is connected with the entrance of the magnetically attractive reative cell, the outlet of the magnetically attractive reative cell and entering for the constant-temperature amplification room
Mouth connection, the outlet of the constant-temperature amplification room are connected with the entrance of the observation ward, the outlet of the observation ward and the waste liquid
The entrance connection in pond.
As a further improvement on the present invention, the thin-film carrier combines provided with vacuole, vacuole combination respectively with
The mixing chamber, magnetically attractive reative cell, the connection of constant-temperature amplification room.
As a further improvement on the present invention, the vacuole combination includes multiple independent vacuoles, and the vacuole includes branch
Fagging and vacuole main body, the vacuole main body and the supporting plate are bonding.
As a further improvement on the present invention, the magnetically attractive reative cell is connected with the waste liquid pool.
As a further improvement on the present invention, the air inlet is connected with air pump.
As a further improvement on the present invention, the thin-film carrier includes topmost thin film and lower film, and the upper strata is thin
Film, lower film heat pressure adhesive, the mixing chamber, magnetically attractive reative cell, constant-temperature amplification room, observation ward be located at the topmost thin film,
Between lower film.
As a further improvement on the present invention, the waste liquid pool includes blotting paper, filter paper and waste liquid chamber, the water suction
Paper, filter paper are separately positioned on the waste liquid intracavitary, and the waste liquid chamber and the thin-film carrier are bonding.
The beneficial effects of the invention are as follows：Pass through such scheme, there is provided a kind of relatively simple, cost is than relatively low micro-fluidic core
Piece, relative to traditional laboratory method, there is the characteristics of integrated level height, small volume, operation is simple, cost is low, applicability is wide.
Brief description of the drawings
Fig. 1 is a kind of schematic diagram of micro-fluidic chip of the present invention.
Fig. 2 is a kind of decomposing schematic representation of the thin-film carrier of micro-fluidic chip of the present invention.
Fig. 3 is a kind of decomposing schematic representation of the vacuole of micro-fluidic chip of the present invention.
Fig. 4 is a kind of decomposing schematic representation of the waste liquid pool of micro-fluidic chip of the present invention.
Embodiment
The invention will be further described for explanation and embodiment below in conjunction with the accompanying drawings.
As shown in Figures 1 to 4, a kind of micro-fluidic chip, including thin-film carrier 9, the thin-film carrier 9 be provided with sample and
Waste liquid after mixing chamber 3, magnetically attractive reative cell 4, constant-temperature amplification room 6, observation ward 7 and collection use that magnetic bead set liquid mixes is simultaneously
The waste liquid pool 8 of scavenging action is played, the entrance of the mixing chamber 3 is connected with plus the adding mouth 1 and air inlet 2 of sample to be detected,
The outlet of the mixing chamber 3 is connected with the entrance of the magnetically attractive reative cell 4, outlet and the constant temperature of the magnetically attractive reative cell 4
The entrance connection of room 6 is expanded, the outlet of the constant-temperature amplification room 6 is connected with the entrance of the observation ward 7, the observation ward 7
Outlet is connected with the entrance of the waste liquid pool 8.
As shown in Figures 1 to 4, the thin-film carrier 9 is provided with the vacuole combination 5 of encapsulation reagent, the vacuole
Combination 5 is connected by transfer runner with the mixing chamber 3, magnetically attractive reative cell 4, constant-temperature amplification room 6 respectively.
As shown in Figures 1 to 4, the vacuole combination 5 includes multiple independent vacuoles, and the vacuole includes the He of supporting plate 53
Vacuole main body 51, the vacuole main body 51 is bonding by double faced adhesive tape 52 with the supporting plate 53, and each independent vacuole is on demand
The capacity for encapsulating reagent makes different size.
As shown in Figures 1 to 4, the magnetically attractive reative cell 4 is connected with the waste liquid pool 8 so that the waste liquid of magnetically attractive reative cell 4
Waste liquid pool 8 can be flowed into.
As shown in Figures 1 to 4, the air inlet 2 is connected with air pump, promotes the flowing of liquid.
As shown in Figures 1 to 4, the thin-film carrier 9 includes topmost thin film 91 and lower film 92, the topmost thin film
91st, the heat pressure adhesive of lower film 92, the mixing chamber 3, magnetically attractive reative cell 4, constant-temperature amplification room 6, observation ward 7 are located at the upper strata
Between film 91, lower film 92, the thin-film carrier 9 can be that bilayer film or plural layers are bonded, as liquid
The carrier of body mixing, transfer and reaction.
As shown in Figures 1 to 4, the waste liquid pool 8 includes blotting paper 83, filter paper 82 and waste liquid chamber 81, the blotting paper
83rd, filter paper 82 is separately positioned in the waste liquid chamber 81, and the waste liquid chamber 81 passes through the phase of double faced adhesive tape 84 with the thin-film carrier 9
Bonding.
A kind of operating process of micro-fluidic chip provided by the invention is：
1st, it is loaded, sample is added into mixing chamber 3 by adding mouth 1；
2nd, mix, discharge magnetic bead set liquid vacuole, incubation is mixed with sample；
3rd, magnetically attractive, mixed liquor being transferred to magnetically attractive reative cell 4, release cleaning solution vacuole is washed, and waste liquid flows into waste liquid pool 8,
Eluent is discharged, nucleic acid is eluted from magnetic bead；
4th, expand, transfer solution to constant-temperature amplification room 6, release amplification liquid carries out constant-temperature amplification；
5th, detect, transfer the solution into observation ward 7 and carry out detection reading.
A kind of micro-fluidic chip provided by the invention, it is a typical PCR application, expands for nucleic acid extraction and constant temperature
Increase, main carriers are thin-film carriers 9, can be applied to PCR tests, gene sequencing, disorder in screening etc., have very high integrated level with
Ease for use；This chip body is combined by thin-film carrier 9 and multiple independent vacuoles, topmost thin film 91 and lower film 92
Hot pressing is bonded to form thin-film carrier 9, and runner and reaction chamber are left in centre, and film loads vacuole, vacuole encapsulation above as pedestal
Reagent, reagent can be discharged by way of extruding, by runner transfer, be mixed, heated, inhaled in reaction chamber
The steps such as magnetic, reaction, detection.
A kind of micro-fluidic chip provided by the invention, there is very high integrated level and ease for use, there is very wide applicability.
Above content is to combine specific preferred embodiment further description made for the present invention, it is impossible to is assert
The specific implementation of the present invention is confined to these explanations.For general technical staff of the technical field of the invention,
On the premise of not departing from present inventive concept, some simple deduction or replace can also be made, should all be considered as belonging to the present invention's
Protection domain.
Claims (7)
- A kind of 1. micro-fluidic chip, it is characterised in that：Including thin-film carrier, the thin-film carrier is provided with sample and magnetic bead set Waste liquid after mixing chamber, magnetically attractive reative cell, constant-temperature amplification room, observation ward and collection use that liquid mixes plays scavenging action simultaneously Waste liquid pool, the entrance of the mixing chamber is connected with plus the adding mouth of sample to be detected and air inlet pressurization are to promote the flowing of liquid Air inlet, the outlet of the mixing chamber is connected with the entrance of the magnetically attractive reative cell, the outlet of the magnetically attractive reative cell and institute The entrance connection of constant-temperature amplification room is stated, the outlet of the constant-temperature amplification room is connected with the entrance of the observation ward, the observation ward Outlet be connected with the entrance of the waste liquid pool.
- 2. micro-fluidic chip according to claim 1, it is characterised in that：The thin-film carrier is provided with encapsulation reagent reacting The vacuole combination of thing, the vacuole combination are connected with the mixing chamber, magnetically attractive reative cell, constant-temperature amplification room respectively.
- 3. micro-fluidic chip according to claim 2, it is characterised in that：The vacuole combination includes multiple independent liquid Bubble, the vacuole include supporting plate and vacuole main body, and the vacuole main body and the supporting plate are bonding.
- 4. micro-fluidic chip according to claim 1, it is characterised in that：The magnetically attractive reative cell connects with the waste liquid pool Connect.
- 5. micro-fluidic chip according to claim 1, it is characterised in that：The air inlet is connected with air pump.
- 6. micro-fluidic chip according to claim 1, it is characterised in that：The thin-film carrier includes topmost thin film and lower floor Film, the topmost thin film, lower film heat pressure adhesive, the mixing chamber, magnetically attractive reative cell, constant-temperature amplification room, observation ward position Between the topmost thin film, lower film.
- 7. micro-fluidic chip according to claim 1, it is characterised in that：The waste liquid pool include blotting paper, filter paper and Waste liquid chamber, the blotting paper, filter paper are separately positioned on the waste liquid intracavitary, and the waste liquid chamber mutually glues with the thin-film carrier Close.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710661755.XA CN107384776A (en) | 2017-08-04 | 2017-08-04 | Micro-fluidic chip |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710661755.XA CN107384776A (en) | 2017-08-04 | 2017-08-04 | Micro-fluidic chip |
Publications (1)
| Publication Number | Publication Date |
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| CN107384776A true CN107384776A (en) | 2017-11-24 |
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| CN201710661755.XA Pending CN107384776A (en) | 2017-08-04 | 2017-08-04 | Micro-fluidic chip |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109847820A (en) * | 2019-04-18 | 2019-06-07 | 天津诺迈科技有限公司 | The pre-packaged device of micro-fluidic chip and application method |
| CN110501491A (en) * | 2019-09-20 | 2019-11-26 | 四川朴澜医疗科技有限公司 | It can support the inclined multichannel incubating device of chip and sample Preparation equipment |
| CN110501514A (en) * | 2019-09-20 | 2019-11-26 | 四川朴澜医疗科技有限公司 | Automatic tester and automatic checkout system |
| CN113109582A (en) * | 2021-04-08 | 2021-07-13 | 埃妥生物科技(杭州)有限公司 | Sample extractor and sample extraction method |
| CN114410448A (en) * | 2022-01-04 | 2022-04-29 | 浙江博毓生物科技有限公司 | Micro-fluidic chip for multi-target nucleic acid detection and detection method |
| CN115433669A (en) * | 2022-08-31 | 2022-12-06 | 深圳市合川医疗科技有限公司 | Pathogen detection micro-fluidic device |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109847820A (en) * | 2019-04-18 | 2019-06-07 | 天津诺迈科技有限公司 | The pre-packaged device of micro-fluidic chip and application method |
| CN110501491A (en) * | 2019-09-20 | 2019-11-26 | 四川朴澜医疗科技有限公司 | It can support the inclined multichannel incubating device of chip and sample Preparation equipment |
| CN110501514A (en) * | 2019-09-20 | 2019-11-26 | 四川朴澜医疗科技有限公司 | Automatic tester and automatic checkout system |
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| CN110501514B (en) * | 2019-09-20 | 2023-12-22 | 四川朴澜医疗科技有限公司 | Automatic detector and automatic detection system |
| CN113109582A (en) * | 2021-04-08 | 2021-07-13 | 埃妥生物科技(杭州)有限公司 | Sample extractor and sample extraction method |
| CN114410448A (en) * | 2022-01-04 | 2022-04-29 | 浙江博毓生物科技有限公司 | Micro-fluidic chip for multi-target nucleic acid detection and detection method |
| CN114410448B (en) * | 2022-01-04 | 2024-02-13 | 浙江博毓生物科技有限公司 | Microfluidic chip for detecting multi-target nucleic acid and detection method |
| CN115433669A (en) * | 2022-08-31 | 2022-12-06 | 深圳市合川医疗科技有限公司 | Pathogen detection micro-fluidic device |
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