CN110495352A - Cultivation of agaricus bisporus material with disease and insect resistance effect - Google Patents
Cultivation of agaricus bisporus material with disease and insect resistance effect Download PDFInfo
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- CN110495352A CN110495352A CN201910893309.0A CN201910893309A CN110495352A CN 110495352 A CN110495352 A CN 110495352A CN 201910893309 A CN201910893309 A CN 201910893309A CN 110495352 A CN110495352 A CN 110495352A
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- agaricus bisporus
- insect resistance
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F5/00—Fertilisers from distillery wastes, molasses, vinasses, sugar plant or similar wastes or residues, e.g. from waste originating from industrial processing of raw material of agricultural origin or derived products thereof
- C05F5/002—Solid waste from mechanical processing of material, e.g. seed coats, olive pits, almond shells, fruit residue, rice hulls
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Abstract
The invention belongs to technical field of edible fungi cultivation, and in particular to after preparation method includes the following steps: that S1 takes pomelo peel extracted, shaddock bark extract is made in the cultivation of agaricus bisporus material with disease and insect resistance effect；After S2 takes cordate houttuynia extracted, houttuynia extract is made；Corncob, bagasse, rye grass are mixed, and are aided with microwave radiation processing in mixed process by S2, and then pressure maintaining is handled under lower pressure, then are boosted to pressure maintaining under condition of high voltage and handled, then carry out degradation treatment, and degradation product is obtained；Degradation product, shaddock bark extract, houttuynia extract are mixed S3, are aided with magnetization treatment in stirring；Cultivation matrix of the invention is full of nutrition, contains multivitamin, amino acid and disease and insect resistance ingredient, and contains magnetic absorption ingredient, can kill pest and disease damage, while can convert insect pest corpse as nutrient, reduces pollution rate.
Description
Technical field
The invention belongs to technical field of edible fungi cultivation, and in particular to the cultivation of agaricus bisporus with disease and insect resistance effect
Material.
Background technique
In Agaricus fungi have rich in nutrients, as protein, amino acid, fat, unsaturated fatty acid, polysaccharide,
Vitamin and microelement etc..In addition, also containing functional activities substances such as carbohydrate, protide, carbohydrates.Therefore, should
Most of fungies have edible value and medical value in category, such as agaricus bisporus, Brazilian mushroom, reddish brown squama mushroom.
Agaricus bisporus is also known as white mushroom, mushroom, mushroom, and European and American countries operator is often referred to as common cultivation mushroom
Or button mushroom.Because of its delicious flavour, meat fertilizer is tender, full of nutrition, deep to be liked there is the title of " world mushroom " by masses, is rich in egg
White matter, polysaccharide, vitamin etc., amino acid composition is more comprehensive, especially rich in a variety of amino acid necessary to human body.
With the rapid development of mushroom industry, cultivated area constantly expands, in Edible Fungi the problem of pest and disease damage
Increasingly prominent, the especially biggish agaricus bisporus of cultivated area, pest and disease damage has exacerbation trend in recent years；Major Diseases have:
Bacterial speck, trichoderma, white gypsum are mould；Major Pests: mushroom mosquito, mushroom fly, mushroom mite etc..So studying a kind of with Resistant
The culture material of evil function is meaningful.As patent No. CN201810222280.9 disclose a kind of Resistant agaricus bisporus culture material and
Its production method and application, but raw material is numerous, is unfavorable for preparing.For another example patent No. CN201510549735.4 discloses a kind of perfume (or spice)
Mushroom cultivation matrix and preparation method thereof, but fermenting agent type is excessive, is unfavorable for control of microorganisms, be easy to cause microorganism dirty
Dye.
Summary of the invention
The present invention is in order to solve the above technical problems, provide the cultivation of agaricus bisporus material with disease and insect resistance effect.
It is realized particular by following technical scheme:
Cultivation of agaricus bisporus material with disease and insect resistance effect, preparation method include the following steps:
After S1 takes pomelo peel extracted, shaddock bark extract is made；
After S2 takes cordate houttuynia extracted, houttuynia extract is made
Corncob, bagasse, rye grass are mixed 10-15min, and are aided with microwave radiation processing in mixed process by S2,
Then pressure maintaining handles 5-7min under the lower pressure of 30-50MPa, then boosts under the condition of high voltage of 140-160MPa at pressure maintaining
8-14min is managed, then carries out degradation treatment, obtains degradation product；
7-13min is mixed in degradation product, shaddock bark extract, houttuynia extract by S3, is aided with magnetization in stirring
Processing.
The extracting method of the pomelo peel are as follows: take pomelo peel to be ground to after drying and sieve with 100 mesh sieve, shaddock peel powder is placed in
In extraction kettle, be added mass fraction be 10-15% citric acid solution, mass fraction be 30-50% ethanol solution, Yu Wendu
It extracts 50-60min under the conditions of being 65-85 DEG C, is centrifugated 5-8min after cooling, taking supernatant in temperature is 45-50 DEG C, vacuum
Degree be 0.1-0.3MPa under the conditions of be concentrated into original volume 1/4 to get；Wherein, the additional amount of citric acid solution is pomelo peel quality
40-60%, the additional amount of ethanol solution is 1-1.3 times of pomelo peel quality.
The extracting method of the cordate houttuynia are as follows: take cordate houttuynia to be placed in the ethanol solution that mass fraction is 40-60%, feed liquid
Than for 1:(16-19), ultrasonic power handles 5-9min under the conditions of being 415-475W, complex enzyme formulation is added to system enzyme activity
For 50000-70000U/L, 7-12min is handled under the conditions of ultrasonic power is 130-180W, adds cordate houttuynia quality 1-5 ‰
Bamboo charcoal powder stir 10-15min, filter, dry to get.
After the inoculum concentrations such as the complex enzyme formulation is cellulase, pectase, protease, amylase are pressed are than mixing, obtain mixed
Synthase；Superoxide dismutase mixing is added, wherein superoxide dismutase inoculum concentration accounting is 20-30ppm.
The frequency 1600-1800MHz of the microwave radiation.
The degradation treatment is 20-30min to be handled in the water vapor atmosphere for be placed in processed material 130-170 DEG C, then be placed in
Natural, sealed soak 10-15min in composite bacteria agent solution, total viable count is (1.5-5.5) × 10 in system6cfu/mL。
The composite bacteria agent solution by inoculum concentration ratio be actinomyces, soft-rot fungi, streptomycete be (12-19): (0.3-0.7):
1。
The operating condition of the magnetization treatment: magnetizing field strength 600-800A/m, power 3-7kW.
The corncob, stalk, rye grass were crushed to 30 meshes before mixing.
The cultivation of agaricus bisporus material with disease and insect resistance effect, including following parts by weight material: corncob 30-40
Part, 20-30 parts of bagasse, 15-18 parts of rye grass, 11-19 parts of shaddock bark extract, 10-14 parts of houttuynia extract.
The utility model has the advantages that
Cultivation matrix of the invention is full of nutrition, contains multivitamin, amino acid and disease and insect resistance ingredient, and contains
Magnetic absorption ingredient can kill pest and disease damage, while can convert insect pest corpse as nutrient, reduce pollution rate；Contain in matrix
There are the cellular construction and polypeptide moiety of the hemicellulose being degraded, cellulose, lignin, mushroom enabled to quickly to absorb,
Nutritional ingredient in mushroom is promoted to increase.
The present invention realizes the fast dewatering in mixed process using microwave radiation, while making surface of material group mutual
Effect, improves the compatibility of material；In conjunction with the processing of low pressure, high pressure, sterilization, enzyme inhibitor effect is not only acted as, wood is also made
The structure of matter cellulose becomes loose, degrades in conjunction with composite bacteria preparation, not only effectively degrades lignocellulosic, also eliminate
Malicious accessory ingredient influences；Using the fuel factor of microwave part carbonaceousfuel component is further heated simultaneously, forms charcoal, pass through
Rational proportion composite bacteria preparation can reach the purpose of lignin degrading, cellulose, hemicellulose, especially lignin degrading,
It can inhibit living contaminants again, specifically avoid the outburst of Bacteria erwinia.
The present invention is by extracting pomelo peel, cordate houttuynia, not only increasing the oxidation resistance of agaricus bisporus and resisting
Sick ability also uses ingredient abundant in pomelo peel and cordate houttuynia, so that respectively materialization life occurs instead in it during the extraction process
It answers, is nutritional ingredient by the conversion wherein contained, the antioxidant content for absorbing, while extracting for agaricus bisporus also helps
In the activity of guarantee nutritional ingredient, and then improve the absorptivity of culture material.
Specific embodiment
Specific embodiments of the present invention will be described in further detail below, but the invention is not limited to these realities
Mode is applied, it is claimed to still fall within the claims in the present invention for any improvement or replacement on the present embodiment essence spirit
Range.
Embodiment 1
Cultivation of agaricus bisporus material with disease and insect resistance effect, preparation method include the following steps:
After S1 takes pomelo peel extracted, shaddock bark extract is made；
After S2 takes cordate houttuynia extracted, houttuynia extract is made
Corncob, bagasse, rye grass are mixed 15min, and are aided with microwave radiation processing in mixed process by S2, then
Pressure maintaining handles 7min under the lower pressure of 50MPa, then boosts to pressure maintaining processing 14min under the condition of high voltage of 160MPa, then into
Row degradation treatment, obtains degradation product；
13min is mixed in degradation product, shaddock bark extract, houttuynia extract by S3, is aided at magnetization in stirring
Reason；
The extracting method of the pomelo peel are as follows: take pomelo peel to be ground to after drying and sieve with 100 mesh sieve, shaddock peel powder is placed in
In extraction kettle, be added mass fraction be 15% citric acid solution, mass fraction be 50% ethanol solution, in temperature be 85 DEG C
Under the conditions of extract 60min, be centrifugated 8min after cooling, take supernatant under the conditions of temperature is 50 DEG C, vacuum degree is 0.3MPa
Be concentrated into original volume 1/4 to get；Wherein, the additional amount of citric acid solution is the 60% of pomelo peel quality, the addition of ethanol solution
Amount is 1.3 times of pomelo peel quality；
The extracting method of the cordate houttuynia are as follows: take cordate houttuynia to be placed in the ethanol solution that mass fraction is 60%, solid-liquid ratio
For 1:19, ultrasonic power handles 9min under the conditions of being 475W, and adding complex enzyme formulation to system enzyme activity is 70000U/L, In
Ultrasonic power handles 12min under the conditions of being 180W, adds the bamboo charcoal powder stirring 15min of cordate houttuynia quality 5 ‰, filters, drying,
To obtain the final product；
After the inoculum concentrations such as the complex enzyme formulation is cellulase, pectase, protease, amylase are pressed are than mixing, obtain mixed
Synthase；Superoxide dismutase mixing is added, wherein superoxide dismutase inoculum concentration accounting is 30ppm；
The frequency 1800MHz of the microwave radiation；
The degradation treatment is 30min to be handled in the water vapor atmosphere for be placed in processed material 170 DEG C, then be placed in composite bacteria agent
Natural, sealed soak 15min in solution, total viable count is 5.5 × 10 in system6cfu/mL；
The composite bacteria agent solution is actinomyces, soft-rot fungi, streptomycete 19:0.7:1 by inoculum concentration ratio；
The operating condition of the magnetization treatment: magnetizing field strength 800A/m, power 7kW；
The corncob, stalk, rye grass were crushed to 30 meshes before mixing.
The cultivation of agaricus bisporus material with disease and insect resistance effect, including following parts by weight material: 40 parts of corncob, sweet
30 parts of bagasse, 18 parts of rye grass, 11-19 parts of shaddock bark extract, 14 parts of houttuynia extract.
Embodiment 2
Cultivation of agaricus bisporus material with disease and insect resistance effect, preparation method include the following steps:
After S1 takes pomelo peel extracted, shaddock bark extract is made；
After S2 takes cordate houttuynia extracted, houttuynia extract is made
Corncob, bagasse, rye grass are mixed 10min, and are aided with microwave radiation processing in mixed process by S2, then
Pressure maintaining handles 5min under the lower pressure of 30MPa, then boosts to pressure maintaining under the condition of high voltage of 140MPa and handle 8min, then carry out
Degradation treatment obtains degradation product；
7min is mixed in degradation product, shaddock bark extract, houttuynia extract by S3, is aided at magnetization in stirring
Reason；
The extracting method of the pomelo peel are as follows: take pomelo peel to be ground to after drying and sieve with 100 mesh sieve, shaddock peel powder is placed in
In extraction kettle, be added mass fraction be 10% citric acid solution, mass fraction be 30% ethanol solution, in temperature be 65 DEG C
Under the conditions of extract 50-60min, be centrifugated 5min after cooling, take supernatant temperature be 45-50 DEG C, vacuum degree 0.1MPa
Under the conditions of be concentrated into original volume 1/4 to get；Wherein, the additional amount of citric acid solution is the 40% of pomelo peel quality, ethanol solution
Additional amount be 1 times of pomelo peel quality；
The extracting method of the cordate houttuynia are as follows: take cordate houttuynia to be placed in the ethanol solution that mass fraction is 40%, solid-liquid ratio
For 1:16, ultrasonic power handles 5min under the conditions of being 415W, and adding complex enzyme formulation to system enzyme activity is 50000U/L, In
Ultrasonic power handles 7min under the conditions of being 130W, adds the bamboo charcoal powder stirring 10min of cordate houttuynia quality 1 ‰, filters, drying,
To obtain the final product；
After the inoculum concentrations such as the complex enzyme formulation is cellulase, pectase, protease, amylase are pressed are than mixing, obtain mixed
Synthase；Superoxide dismutase mixing is added, wherein superoxide dismutase inoculum concentration accounting is 20ppm；
The frequency 1600MHz of the microwave radiation；
The degradation treatment is 20min to be handled in the water vapor atmosphere for be placed in processed material 130 DEG C, then be placed in composite bacteria agent
Natural, sealed soak 10min in solution, total viable count is 1.5 × 10 in system6cfu/mL；
The composite bacteria agent solution is actinomyces, soft-rot fungi, streptomycete 12:0.3:1 by inoculum concentration ratio；
The operating condition of the magnetization treatment: magnetizing field strength 600A/m, power 3kW；
The corncob, stalk, rye grass were crushed to 30 meshes before mixing.
The cultivation of agaricus bisporus material with disease and insect resistance effect, including following parts by weight material: 30 parts of corncob, sweet
20 parts of bagasse, 15 parts of rye grass, 11 parts of shaddock bark extract, 10 parts of houttuynia extract.
Embodiment 3
Cultivation of agaricus bisporus material with disease and insect resistance effect, preparation method include the following steps:
After S1 takes pomelo peel extracted, shaddock bark extract is made；
After S2 takes cordate houttuynia extracted, houttuynia extract is made
Corncob, bagasse, rye grass are mixed 12min, and are aided with microwave radiation processing in mixed process by S2, then
Pressure maintaining handles 6min under the lower pressure of 40MPa, then boosts to pressure maintaining processing 11min under the condition of high voltage of 150MPa, then into
Row degradation treatment, obtains degradation product；
10min is mixed in degradation product, shaddock bark extract, houttuynia extract by S3, is aided at magnetization in stirring
Reason；
The extracting method of the pomelo peel are as follows: take pomelo peel to be ground to after drying and sieve with 100 mesh sieve, shaddock peel powder is placed in
In extraction kettle, be added mass fraction be 12% citric acid solution, mass fraction be 40% ethanol solution, in temperature be 75 DEG C
Under the conditions of extract 55min, be centrifugated 7min after cooling, take supernatant under the conditions of temperature is 48 DEG C, vacuum degree is 0.2MPa
Be concentrated into original volume 1/4 to get；Wherein, the additional amount of citric acid solution is the 50% of pomelo peel quality, the addition of ethanol solution
Amount is 1.1 times of pomelo peel quality；
The extracting method of the cordate houttuynia are as follows: take cordate houttuynia to be placed in the ethanol solution that mass fraction is 50%, solid-liquid ratio
For 1:17, ultrasonic power handles 7min under the conditions of being 445W, and adding complex enzyme formulation to system enzyme activity is 60000U/L, In
Ultrasonic power handles 9min under the conditions of being 140W, adds the bamboo charcoal powder stirring 12min of cordate houttuynia quality 3 ‰, filters, drying,
To obtain the final product；
After the inoculum concentrations such as the complex enzyme formulation is cellulase, pectase, protease, amylase are pressed are than mixing, obtain mixed
Synthase；Superoxide dismutase mixing is added, wherein superoxide dismutase inoculum concentration accounting is 25ppm；
The frequency 1700MHz of the microwave radiation；
The degradation treatment is 25min to be handled in the water vapor atmosphere for be placed in processed material 160 DEG C, then be placed in composite bacteria agent
Natural, sealed soak 13min in solution, total viable count is 3.5 × 10 in system6cfu/mL；
The composite bacteria agent solution is actinomyces, soft-rot fungi, streptomycete 15:0.4:1 by inoculum concentration ratio；
The operating condition of the magnetization treatment: magnetizing field strength 700A/m, power 5kW；
The corncob, stalk, rye grass were crushed to 30 meshes before mixing.
The cultivation of agaricus bisporus material with disease and insect resistance effect, including following parts by weight material: 35 parts of corncob, sweet
25 parts of bagasse, 16 parts of rye grass, 14 parts of shaddock bark extract, 12 parts of houttuynia extract.
Test example 1
The application is cultivated using the cultivation matrix of following test group, and compares the feelings of each group yield and biological transformation ratio
Condition, the specific method is as follows:
Cultivation matrix selection is as follows:
Test group 1: embodiment 3；
Test group 2: the difference with embodiment 3 is: without magnetization；
Test group 3: the difference with embodiment 3 is: without microwave radiation；
Test group 4: the difference with embodiment 3 is: being not added with shaddock bark extract；
Test group 5: the difference with embodiment 3 is: being not added with houttuynia extract；
Cultural method: 1) feeding and sowing: every square metre of feeding 110kg, bacterial classification usage amount be compost weight 0.7%~
0.8%.It is flattened in feeding, while covering thin film moisturizing, carry out bacterium germination and management of producing mushroom later；2) bacterium germination and management of producing mushroom:
During bacterium germination, the temperature of compost is kept a close eye on, control compost temperature is at 24~26 DEG C, relative air humidity 95%, dioxy
Change concentration of carbon and is not less than 3000mg/kg；Mycelia is long to overburden layer 2/3 or so, and mushroom house starts to cool down, and within 5~6d time, makes mushroom
The temperature in room drops to 17 DEG C from 22 DEG C, and gas concentration lwevel drops to 1000mg/kg, cooling extent and draft power from 5000mg/kg
Degree is gentle, promotes mycelia kink at fructification；When the upper mushroom flower bud thumb size of mushroom bed, water spray promotes fructification to expand.Mushroom
Temperature control in room is at 17 DEG C, gas concentration lwevel 1000mg/kg or so, relative air humidity 90% or so；
Test result: test group 1 is whole not to occur disease, the test group 2-5 mould disease incidence of whole process wart spore is 7.8%,
10.3%, 9.6%, 12.1%, in addition test group 2, test group 4 have a small amount of black spot.
Protein content determination uses GB5009.5-2010 " measurement of national food safety standard Protein in Food "；
The determination of fat uses GB/T15674-2009 " measurement of crude fat content in edible mushroom "；Contents of Amino Acids uses
GB/T5009.124-2003 " measurement of amino acid in food "；Crude fiber content measurement " is planted using GB/T5009.10-2003
Coarse-fibred measurement in species food "；Soluble sugar content measurement is using " the Thick many candies content in edible mushroom of NY/T1676-2008
Measurement "；Content of ashes uses GB/T12532-2008 " edible mushroom ash determination "；Vitamin C content, which is surveyed, uses GB/T6195
~1986 " water fruits and vegetables Vitamin C content measuring method (2,6- dichloroindophenol titrations) ", as shown in table 1:
Claims (9)
1. the cultivation of agaricus bisporus material with disease and insect resistance effect, which is characterized in that preparation method includes the following steps:
After S1 takes pomelo peel extracted, shaddock bark extract is made；
After S2 takes cordate houttuynia extracted, houttuynia extract is made
Corncob, bagasse, rye grass are mixed 10-15min, and are aided with microwave radiation processing in mixed process by S2, then
Pressure maintaining handles 5-7min under the lower pressure of 30-50MPa, then boosts to pressure maintaining under the condition of high voltage of 140-160MPa and handle 8-
14min, then degradation treatment is carried out, obtain degradation product；
7-13min is mixed in degradation product, shaddock bark extract, houttuynia extract by S3, is aided with magnetization treatment in stirring.
2. as described in claim 1 with the cultivation of agaricus bisporus material of disease and insect resistance effect, which is characterized in that the pomelo peel
Extracting method are as follows: take pomelo peel to be ground to after drying and sieve with 100 mesh sieve, shaddock peel powder is placed in extraction kettle, quality point is added
The ethanol solution that citric acid solution, the mass fraction that number is 10-15% are 30-50% extracts under the conditions of being 65-85 DEG C in temperature
50-60min is centrifugated 5-8min after cooling, takes supernatant temperature is 45-50 DEG C, vacuum degree is 0.1-0.3MPa condition
Under be concentrated into original volume 1/4 to get；Wherein, the additional amount of citric acid solution is the 40-60% of pomelo peel quality, ethanol solution
Additional amount be 1-1.3 times of pomelo peel quality.
3. as described in claim 1 with the cultivation of agaricus bisporus material of disease and insect resistance effect, which is characterized in that the cordate houttuynia
Extracting method are as follows: take cordate houttuynia to be placed in the ethanol solution that mass fraction is 40-60%, solid-liquid ratio 1:(16-19), ultrasonic function
Rate handles 5-9min under the conditions of being 415-475W, and adding complex enzyme formulation to system enzyme activity is 50000-70000U/L, In
Ultrasonic power handles 7-12min under the conditions of being 130-180W, adds the bamboo charcoal powder stirring 10- of cordate houttuynia quality 1-5 ‰
15min, filter, dry to get.
4. as claimed in claim 3 with the cultivation of agaricus bisporus material of disease and insect resistance effect, which is characterized in that the complex enzyme system
After the inoculum concentrations such as agent is cellulase, pectase, protease, amylase are pressed are than mixing, mixed enzyme is obtained；Add super oxygenization disproportionation
Enzyme mixing, wherein superoxide dismutase inoculum concentration accounting is 20-30ppm.
5. as described in claim 1 with the cultivation of agaricus bisporus material of disease and insect resistance effect, which is characterized in that the microwave radiation
Frequency 1600-1800MHz.
6. as described in claim 1 with the cultivation of agaricus bisporus material of disease and insect resistance effect, which is characterized in that the degradation treatment
It is 20-30min to be handled in the water vapor atmosphere for be placed in processed material 130-170 DEG C, then be placed in natural, close in composite bacteria agent solution
Envelope impregnates 10-15min, and total viable count is (1.5-5.5) × 10 in system6cfu/mL。
7. as claimed in claim 6 with the cultivation of agaricus bisporus material of disease and insect resistance effect, which is characterized in that the composite bacteria agent
Solution by inoculum concentration ratio be actinomyces, soft-rot fungi, streptomycete be (12-19): (0.3-0.7): 1.
8. as described in claim 1 with the cultivation of agaricus bisporus material of disease and insect resistance effect, which is characterized in that the magnetization treatment
Operating condition: magnetizing field strength 600-800A/m, power 3-7kW.
9. as described in claim 1 with the cultivation of agaricus bisporus material of disease and insect resistance effect, which is characterized in that described with disease-resistant
The cultivation of agaricus bisporus material of insect pest effect, including following parts by weight material: 30-40 parts of corncob, 20-30 parts of bagasse, rye
15-18 parts careless, 11-19 parts of shaddock bark extract, 10-14 parts of houttuynia extract.
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