CN110412000B - 基于十元瓜环检测l-色氨酸的荧光探针及其检测方法 - Google Patents
基于十元瓜环检测l-色氨酸的荧光探针及其检测方法 Download PDFInfo
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Abstract
本发明公开了一种基于十元瓜环检测L‑色氨酸的荧光探针及其检测方法，其分子式为C60H60N40O20@C20H18ClNO4，化学结构式如附图1所示。其检测方法是将所述荧光探针加水稀释，得探针标准溶液，然后向探针标准溶液中加入待测物水溶液，静置5‑20s后以固定激发波长350nm进行荧光发射光谱测定，并绘制激发出的该激光波长处的荧光强度的变化曲线；根据变化曲线计算荧光探针溶液中加入待测物水溶液前后分别对应538.95nm下的荧光发射光谱强度变化，即可对L‑色氨酸进行检测。本发明是一种新型的荧光探针，具有能检测水中的L‑色氨酸的特点，且具有灵敏度高、检测成本低、样品处理简单、操作方便、测定快速以及实时检测的特点。
Description
技术领域
本发明涉及一种检测L-色氨酸的荧光探针及其检测方法，特别是一种基于十元瓜环检测L-色氨酸的荧光探针及其检测方法。
背景技术
氨基酸在生物体成长发育过程中发挥着极其重要的其作用：1.是人体组织的构成部分；2.构成人体内的各种物质；3.供给热量；4.免疫调节；5.作为体内重要的载体,起者运输的作用；6.氧化功能。
氨基酸是蛋白质的基本组成物质,摄入氨基酸是人体获得氮源的唯一方式,人体吸收氨基酸后一部分被直接用来合成蛋白质，一部分被氧化分解,其中含氮部分用来合成其他必须氨基酸，一部分作为能量被分解掉,以尿素的形式排出体外。
因此,对食物中氨基酸含量及其种类的检测显得尤为重要，而荧光探针是一种新型的检测试剂，由于其具有较高灵敏度、较低检测成本、样品处理简单、操作方便、测定快速以及实时检测的优点而备受人们的青睐。
L-色氨酸又名α-氨基吲哚基丙酸，分子式:C11H12N2O2，白色至黄白色晶体或结晶性粉末。无臭或微臭，长时间光照则着色。与酸在暗处加热较稳定。与其他氨基酸、糖类、醛类共存时极易分解。用作食品强化剂、抗氧剂。也用于医药等方面。由吲哚醛合成而得，也可由胰蛋白酶分解、合成制取。因此，研究新型的检测L-色氨酸用的荧光探针及相关检测方法很有必要。
发明内容
本发明的目的在于，提供一种基于十元瓜环检测L-色氨酸的荧光探针及其检测方法。本发明是一种新型的荧光探针，具有能检测水中的L-色氨酸的特点，且具有灵敏度高、检测成本低、样品处理简单、操作方便、测定快速以及实时检测的特点。
本发明的技术方案：一种基于十元瓜环检测L-色氨酸的荧光探针，其分子式为C60H60N40O20@C20H18ClNO4，化学结构式如附图1所示。
前述的基于十元瓜环检测L-色氨酸的荧光探针，所述荧光探针是由十元瓜环和盐酸小檗碱制成。
前述的基于十元瓜环检测L-色氨酸的荧光探针，所述荧光探针的具体制备方法包括如下步骤：
(1)将十元瓜环加水溶解，得溶液A；
(2)将盐酸小檗碱加水溶解，得溶液B；
(3)将溶液A与溶液B混合，常温反应，即得荧光探针。
前述的基于十元瓜环检测L-色氨酸的荧光探针，步骤(1)所述溶液A的浓度为1.0×10-4mol/L。
前述的基于十元瓜环检测L-色氨酸的荧光探针，步骤(2)所述溶液B的浓度为1.0×10-3mol/L。
前述的基于十元瓜环检测L-色氨酸的荧光探针，步骤(2)所述溶液A和溶液B混合时，混合液中十元瓜环与盐酸小檗碱的摩尔比为1:0.5-2。
前述的基于十元瓜环检测L-色氨酸的荧光探针，所述混合液中十元瓜环与盐酸小檗碱的摩尔比为1:1。
一种前述的荧光探针检测L-色氨酸的方法，是将所述荧光探针加水稀释，得探针标准溶液，然后向探针标准溶液中加入待测物水溶液，静置5-20s后以固定激发波长350nm进行荧光发射光谱测定，并绘制激发出的该激光波长处的荧光强度的变化曲线；根据变化曲线计算荧光探针溶液中加入待测物水溶液前后分别对应538.95nm下的荧光发射光谱强度变化，即可对L-色氨酸进行检测。
前述的荧光探针检测L-色氨酸的方法，所述探针标准溶液的浓度为2.0×10-5mol/L。
前述的荧光探针检测L-色氨酸的方法，当加入所述待测物水溶液前后探针标准溶液在538.95nm下的荧光发射光谱强度降低，则表明待测物水溶液中含有L-色氨酸，否则不含。
本发明的有益效果
本发明的荧光探针是一种新型的荧光探针，能够对水溶液中的L-色氨酸进行检测；此外，本发明的荧光探针检出限低至4.750×10-6mol/L，具有灵敏度高的优点；同时，荧光探针只需以十元瓜环和盐酸小檗碱为原料通过简单方法即可制备，并以简单的方法进行检测，因此，具有检测成本低，样品处理简单，操作方便的优点；再者，由于检测操作简单，能够实时且快速的检测。
为验证本发明的有益效果，发明人进行了大量的实验研究，部分实验过程和结果如下：
实验例1探究十元瓜环和盐酸小檗碱的合适摩尔比
为了探究十元瓜环和盐酸小檗碱所形成探针的合适摩尔比，采用紫外吸收光法谱和荧光光谱法对主客体之间的相互作用进行了考察。
摩尔比法测定各体系之间的紫外吸收光谱和荧光谱和荧光光谱数据，具体方法为：将盐酸小檗碱和十元瓜环分别配制成1.0mmol/L和0.1mmol/L的水溶液备用，固定客体浓度为0.02mmol/L，改变十元瓜环的浓度，配置N十元瓜环/N盐酸小檗碱为0、0.2、0.4、0.6、0.8、1.0、1.2、1.4、1.6、1.8、2.0、2.2、2.4、2.6、2.8、3.0的水溶液，在室温下测定溶液的紫外-可见吸收光谱；固定客体浓度为0.02mmol/L，改变十元瓜环的浓度，配置N十元瓜环/N盐酸小檗碱为0、0.2、0.4、0.6、0.8、1.0、1.2、1.4、1.6、1.8、2.0、2.2、2.4、2.6、2.8、3.0的水溶液，在激发波长为350nm，激发狭缝为10nm，发射狭缝为10nm，电压为560V的条件下测定溶液的荧光发射光谱，结果如附图2所示。
实验例2定量分析
向本发明制得的浓度为2.0×10-5mol/L的荧光探针标准溶液中加入不同体积分数的含有L-色氨酸的溶液进行检测，检测结果如图4和5所示，可以看出，加入不同体积分数后标准溶液中L-色氨酸的浓度也不相同，不同浓度的L-色氨酸可使荧光探针溶液发生不同程度的荧光减弱，而L-色氨酸响应的线性范围为(1.0-30.0)×10-5mol/L，检出限为4.750×10-6mol/L(如图6)。
附图说明
图1为本发明荧光探针的分子结构式；
图2为十元瓜环与盐酸小檗碱摩尔比法；
图3为探针标准溶液加入含有不同L-氨基酸的溶液时的荧光光谱曲线；
图4为探针标准溶液加入不同浓度含有L-色氨酸的溶液时的荧光光谱曲线；
图5为探针标准溶液加入不同浓度含有L-色氨酸的溶液时的荧光光谱强度；
图6为本发明荧光探针检出限模拟图；
图7为盐酸小檗碱加入不同的L-氨基酸的荧光强度变化谱图；
具体实施方式
下面结合实施例对本发明作进一步的说明，但并不作为对本发明限制的依据。
本发明的实施例
实施例1：基于十元瓜环检测L-色氨酸的荧光探针的制备方法，步骤如下：
(1)将十元瓜环加水溶解，得浓度为1.0×10-4mol/L的溶液A；
(2)将盐酸小檗碱加水溶解，得浓度为1.0×10-3mol/L的溶液B；
(3)将溶液A与溶液B混合，混合液中十元瓜环与盐酸小檗碱的摩尔比为1:1，常温反应，即得荧光探针。
实施例2：基于十元瓜环检测L-色氨酸的荧光探针的制备方法，步骤如下：
(1)将十元瓜环加水溶解，得浓度为1.0×10-4mol/L的溶液A；
(2)将盐酸小檗碱加水溶解，得浓度为1.0×10-3mol/L的溶液B；
(3)将溶液A与溶液B混合，混合液中十元瓜环与盐酸小檗碱的摩尔比为1:0.5，常温反应，即得荧光探针。
实施例3：基于十元瓜环检测L-色氨酸的荧光探针的制备方法，步骤如下：
(1)将十元瓜环加水溶解，得浓度为1.0×10-4mol/L的溶液A；
(2)将盐酸小檗碱加水溶解，得浓度为1.0×10-3mol/L的溶液B；
(3)将溶液A与溶液B混合，混合液中十元瓜环与盐酸小檗碱的摩尔比为1:2，常温反应，即得荧光探针。
实施例4：检测L-色氨酸的方法，步骤如下：
(1)将本发明的荧光探针加水稀释成浓度为2.0×10-5mol/L的探针标准溶液；
(2)将待测物加水溶解，并将待测物水溶液加入探针标准溶液中，静置10s后以固定激发波长350nm进行荧光发射光谱测定，并绘制激发出的该激光波长处的荧光强度的变化曲线，当加入所述待测物水溶液前后探针标准溶液在538.95nm下的荧光发射光谱强度降低，则表明待测物水溶液中含有L-色氨酸，否则不含。
实施例5：检测L-色氨酸的方法，步骤如下：
(1)将本发明的荧光探针加水稀释成浓度为2.0×10-5mol/L的探针标准溶液；
(2)将待测物加水溶解，并将待测物水溶液加入探针标准溶液中，静置5s后以固定激发波长350nm进行荧光发射光谱测定，并绘制激发出的该激光波长处的荧光强度的变化曲线，当加入所述待测物水溶液前后探针标准溶液在538.95nm下的荧光发射光谱强度降低，则表明待测物水溶液中含有L-色氨酸，否则不含。
实施例6：检测L-色氨酸的方法，步骤如下：
(1)将本发明的荧光探针加水稀释成浓度为2.0×10-5mol/L的探针标准溶液；
(2)将待测物加水溶解，并将待测物水溶液加入探针标准溶液中，静置20s后以固定激发波长350nm进行荧光发射光谱测定，并绘制激发出的该激光波长处的荧光强度的变化曲线，当加入所述待测物水溶液前后探针标准溶液在538.95nm下的荧光发射光谱强度降低，则表明待测物水溶液中含有L-色氨酸，否则不含。
Claims (8)
1.一种基于十元瓜环的荧光探针检测L-色氨酸的方法，其特征在于：所述荧光探针的分子式为C60H60N40O20@C20H18ClNO4，化学结构式为：
所述的荧光探针检测L-色氨酸的方法：是将所述荧光探针加水稀释，得探针标准溶液，然后向探针标准溶液中加入待测物水溶液，静置5-20s后以固定激发波长350nm进行荧光发射光谱测定，并绘制激发出的350nm激发 波长处的荧光强度的变化曲线；根据变化曲线计算荧光探针溶液中加入待测物水溶液前后分别对应538.95nm下的荧光发射光谱强度变化，即可对L-色氨酸进行检测；
所述荧光探针是由十元瓜环和盐酸小檗碱制成。
2.根据权利要求1所述的基于十元瓜环的荧光探针检测L-色氨酸的方法，其特征在于，所述荧光探针的具体制备方法包括如下步骤：
(1)将十元瓜环加水溶解，得溶液A；
(2)将盐酸小檗碱加水溶解，得溶液B；
(3)将溶液A与溶液B混合，常温反应，即得荧光探针。
3.根据权利要求2所述的基于十元瓜环的荧光探针检测L-色氨酸的方法，其特征在于：步骤(1)所述溶液A的浓度为1.0×10-4mol/L。
4.根据权利要求2所述的基于十元瓜环的荧光探针检测L-色氨酸的方法，其特征在于：步骤(2)所述溶液B的浓度为1.0×10-3mol/L。
5.根据权利要求2所述的基于十元瓜环的荧光探针检测L-色氨酸的方法，其特征在于：步骤(2)所述溶液A和溶液B混合时，混合液中十元瓜环与盐酸小檗碱的摩尔比为1:0.5-2。
6.根据权利要求5所述的基于十元瓜环的荧光探针检测L-色氨酸的方法，其特征在于：所述混合液中十元瓜环与盐酸小檗碱的摩尔比为1:1。
7.根据权利要求1所述的基于十元瓜环的荧光探针检测L-色氨酸的方法，其特征在于：所述探针标准溶液的浓度为2.0×10-5mol/L。
8.根据权利要求1所述的基于十元瓜环的荧光探针检测L-色氨酸的方法，其特征在于：当加入所述待测物水溶液前后探针标准溶液在538.95nm下的荧光发射光谱强度降低，则表明待测物水溶液中含有L-色氨酸，否则不含。
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