CN110411999B - 基于八元瓜环检测l-苯丙氨酸的荧光探针及其检测方法 - Google Patents
基于八元瓜环检测l-苯丙氨酸的荧光探针及其检测方法 Download PDFInfo
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- C07D455/03—Heterocyclic compounds containing quinolizine ring systems, e.g. emetine alkaloids, protoberberine; Alkylenedioxy derivatives of dibenzo [a, g] quinolizines, e.g. berberine containing quinolizine ring systems directly condensed with at least one six-membered carbocyclic ring, e.g. protoberberine; Alkylenedioxy derivatives of dibenzo [a, g] quinolizines, e.g. berberine
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Abstract
本发明公开了一种基于八元瓜环检测L‑苯丙氨酸的荧光探针及其检测方法，其分子式为C48H48N32O16@C20H18ClNO4，化学结构式如附图1所示。其检测方法是将所述荧光探针加水稀释，得探针标准溶液，然后向探针标准溶液中加入待测物水溶液，静置5‑20s后以固定激发波长350nm进行荧光发射光谱测定，并绘制激发出的该激光波长处的荧光强度的变化曲线；根据变化曲线计算荧光探针溶液中加入待测物水溶液前后分别对应521.04nm下的荧光发射光谱强度变化，即可对L‑苯丙氨酸进行检测。本发明是一种新型的荧光探针，具有能检测水中的L‑苯丙氨酸的特点，且具有灵敏度高、检测成本低、样品处理简单、操作方便、测定快速以及实时检测的特点。
Description
技术领域
本发明涉及一种检测L-苯丙氨酸的荧光探针及其检测方法，特别是一种基于八元瓜环检测L-苯丙氨酸的荧光探针及其检测方法。
背景技术
氨基酸在生物体成长发育过程中发挥着极其重要的其作用：1.是人体组织的构成部分；2.构成人体内的各种物质；3.供给热量；4.免疫调节；5.作为体内重要的载体,起者运输的作用；6.氧化功能。
氨基酸是蛋白质的基本组成物质,摄入氨基酸是人体获得氮源的唯一方式,人体吸收氨基酸后一部分被直接用来合成蛋白质，一部分被氧化分解,其中含氮部分用来合成其他必须氨基酸，一部分作为能量被分解掉,以尿素的形式排出体外。
因此,对食物中氨基酸含量及其种类的检测显得尤为重要，而荧光探针是一种新型的检测试剂，由于其具有较高灵敏度、较低检测成本、样品处理简单、操作方便、测定快速以及实时检测的优点而备受人们的青睐。
L-苯丙氨酸(英文名：L-Phenylalanine)为无色至白色片状晶体或白色结晶性粉末，是一种营养增补剂，是必需氨基酸之一。在体内大部分经苯丙氨酸羟化酶催化作用氧化成酪氨酸，并与酪氨酸一起合成重要的神经递质和激素，参与机体糖代谢和脂肪代谢。因此，研究新型的检测L-苯丙氨酸用的荧光探针及相关检测方法很有必要。
发明内容
本发明的目的在于，提供一种基于八元瓜环检测L-苯丙氨酸的荧光探针及其检测方法。本发明是一种新型的荧光探针，具有能检测水中的L-苯丙氨酸的特点，且具有灵敏度高、检测成本低、样品处理简单、操作方便、测定快速以及实时检测的特点。
本发明的技术方案：一种基于八元瓜环检测L-苯丙氨酸的荧光探针，其分子式为C48H48N32O16@C20H18ClNO4，化学结构式如附图1所示。
前述的基于八元瓜环检测L-苯丙氨酸的荧光探针，所述荧光探针是由八元瓜环和盐酸小檗碱制成。
前述的基于八元瓜环检测L-苯丙氨酸的荧光探针，所述荧光探针的具体制备方法包括如下步骤：
(1)将八元瓜环加水溶解，得溶液A；
(2)将盐酸小檗碱加水溶解，得溶液B；
(3)将溶液A与溶液B混合，常温反应，即得荧光探针。
前述的基于八元瓜环检测L-苯丙氨酸的荧光探针，步骤(1)所述溶液A的浓度为1.0×10-4mol/L。
前述的基于八元瓜环检测L-苯丙氨酸的荧光探针，步骤(2)所述溶液B的浓度为1.0×10-3mol/L。
前述的基于八元瓜环检测L-苯丙氨酸的荧光探针，步骤(2)所述溶液A和溶液B混合时，混合液中八元瓜环与盐酸小檗碱的摩尔比为1:0.5-3。
前述的基于八元瓜环检测L-苯丙氨酸的荧光探针，所述混合液中八元瓜环与盐酸小檗碱的摩尔比为1:2。
一种前述的荧光探针检测L-苯丙氨酸的方法，是将所述荧光探针加水稀释，得探针标准溶液，然后向探针标准溶液中加入待测物水溶液，静置5-20s后以固定激发波长350nm进行荧光发射光谱测定，并绘制激发出的该激光波长处的荧光强度的变化曲线；根据变化曲线计算荧光探针溶液中加入待测物水溶液前后分别对应521.04nm下的荧光发射光谱强度变化，即可对L-苯丙氨酸进行检测。
前述的荧光探针检测L-苯丙氨酸的方法，所述探针标准溶液的浓度为2.0×10- 5mol/L。
前述的荧光探针检测L-苯丙氨酸的方法，当加入所述待测物水溶液前后探针标准溶液在521.04nm下的荧光发射光谱强度增强，则表明待测物水溶液中含有L-苯丙氨酸，否则不含。
本发明的有益效果
本发明的荧光探针是一种新型的荧光探针，能够对水溶液中的L-苯丙氨酸进行检测；此外，本发明的荧光探针检出限低至1.7398×10-6mol/L，具有灵敏度高的优点；同时，荧光探针只需以八元瓜环和盐酸小檗碱为原料通过简单方法即可制备，并以简单的方法进行检测，因此，具有检测成本低，样品处理简单，操作方便的优点；再者，由于检测操作简单，能够实时且快速的检测。
为验证本发明的有益效果，发明人进行了大量的实验研究，部分实验过程和结果如下：
实验例1探究八元瓜环和盐酸小檗碱的合适摩尔比
为了探究八元瓜环和盐酸小檗碱所形成探针的合适摩尔比，采用紫外吸收光法谱和荧光光谱法对主客体之间的相互作用进行了考察。
摩尔比法测定各体系之间的紫外吸收光谱和荧光谱和荧光光谱数据，具体方法为：将盐酸小檗碱和八元瓜环分别配制成1.0mmol/L和0.1mmol/L的水溶液备用，固定客体浓度为0.02mmol/L，改变八元瓜环的浓度，配置N八元瓜环/N盐酸小檗碱为0、0.1、0.2、0.3、0.5、0.5、0.6、0.7、0.8、0.9、1.0、1.1、1.2、1.3、1.4、1.5的水溶液，在室温下测定溶液的紫外-可见吸收光谱；固定客体浓度为0.02mmol/L，改变八元瓜环的浓度，配置N八元瓜环/N盐酸小檗碱为0、0.1、0.2、0.3、0.4、0.5、0.6、0.7、0.8、0.9、1.0、1.1、1.2、1.3、1.4、1.5的水溶液，在激发波长为350nm，激发狭缝为5nm，发射狭缝为5nm，电压为540V的条件下测定溶液的荧光发射光谱，随后采用等摩尔连续变化法(JOB法)测定体系之间的紫外吸收光谱和荧光谱，固定主客体的总浓度为4.0mmol/L不变，通过不断改变主客体之间的物质的量之比，配制出一系列不同摩尔比N八元瓜环/(N八元瓜环+N盐酸小檗
碱)＝0.1、0.2……0.8、0.9、1.0的待测溶液，并按此方法，测定紫外吸收光谱和荧光谱。
实验例2定量分析
向本发明制得的浓度为2.0×10-5mol/L的荧光探针标准溶液中加入不同体积分数的含有L-苯丙氨酸的溶液进行检测，检测结果如图4、5所示，可以看出，加入不同体积分数后标准溶液中L-苯丙氨酸的浓度也不相同，不同浓度的L-苯丙氨酸可使荧光探针溶液发生不同程度的荧光增敏，而L-苯丙氨酸响应的线性范围为(2.0-30.0)×10-5mol/L，检出限为1.7398×10-6mol/L(如图6)。
附图说明
图1为本发明荧光探针的分子结构式；
图2为八元瓜环与盐酸小檗碱摩尔比法；
图3为探针标准溶液加入含有不同L-氨基酸的溶液时的荧光光谱曲线；
图4为探针标准溶液加入不同浓度含有L-苯丙氨酸的溶液时的荧光光谱曲线；
图5为探针标准溶液加入不同浓度含有L-苯丙氨酸的溶液时的荧光光谱强度；
图6为本发明荧光探针检出限模拟图；
图7为探针标准溶液加入含有不同L-氨基酸的溶液时的紫外光谱曲线；
具体实施方式
下面结合实施例对本发明作进一步的说明，但并不作为对本发明限制的依据。
本发明的实施例
实施例1：基于八元瓜环检测L-苯丙氨酸的荧光探针的制备方法，步骤如下：
(1)将八元瓜环加水溶解，得浓度为1.0×10-4mol/L的溶液A；
(2)将盐酸小檗碱加水溶解，得浓度为1.0×10-3mol/L的溶液B；
(3)将溶液A与溶液B混合，混合液中八元瓜环与盐酸小檗碱的摩尔比为1:2，常温反应，即得荧光探针。
实施例2：基于八元瓜环检测L-苯丙氨酸的荧光探针的制备方法，步骤如下：
(1)将八元瓜环加水溶解，得浓度为1.0×10-4mol/L的溶液A；
(2)将盐酸小檗碱加水溶解，得浓度为1.0×10-3mol/L的溶液B；
(3)将溶液A与溶液B混合，混合液中八元瓜环与盐酸小檗碱的摩尔比为1:0.5，常温反应，即得荧光探针。
实施例3：基于八元瓜环检测L-苯丙氨酸的荧光探针的制备方法，步骤如下：
(1)将八元瓜环加水溶解，得浓度为1.0×10-4mol/L的溶液A；
(2)将盐酸小檗碱加水溶解，得浓度为1.0×10-3mol/L的溶液B；
(3)将溶液A与溶液B混合，混合液中八元瓜环与盐酸小檗碱的摩尔比为1:3，常温反应，即得荧光探针。
实施例4：检测L-苯丙氨酸的方法，步骤如下：
(1)将本发明的荧光探针加水稀释成浓度为2.0×10-5mol/L的探针标准溶液；
(2)将待测物加水溶解，并将待测物水溶液加入探针标准溶液中，静置10s后以固定激发波长350nm进行荧光发射光谱测定，并绘制激发出的该激光波长处的荧光强度的变化曲线，当加入所述待测物水溶液前后探针标准溶液在521.04nm下的荧光发射光谱强度增强，则表明待测物水溶液中含有L-苯丙氨酸，否则不含。
实施例5：检测L-苯丙氨酸的方法，步骤如下：
(1)将本发明的荧光探针加水稀释成浓度为2.0×10-5mol/L的探针标准溶液；
(2)将待测物加水溶解，并将待测物水溶液加入探针标准溶液中，静置5s后以固定激发波长350nm进行荧光发射光谱测定，并绘制激发出的该激光波长处的荧光强度的变化曲线，当加入所述待测物水溶液前后探针标准溶液在521.04nm下的荧光发射光谱强度增强，则表明待测物水溶液中含有L-苯丙氨酸，否则不含。
实施例6：检测L-苯丙氨酸的方法，步骤如下：
(1)将本发明的荧光探针加水稀释成浓度为2.0×10-5mol/L的探针标准溶液；
(2)将待测物加水溶解，并将待测物水溶液加入探针标准溶液中，静置20s后以固定激发波长350nm进行荧光发射光谱测定，并绘制激发出的该激光波长处的荧光强度的变化曲线，当加入所述待测物水溶液前后探针标准溶液在521.04nm下的荧光发射光谱强度增强，则表明待测物水溶液中含有L-苯丙氨酸，否则不含。
Claims (2)
1.一种基于八元瓜环荧光探针检测L-苯丙氨酸的应用，其特征在于：所述荧光探针的分子式为C48H48N32O16@2C20H18ClNO4，化学结构式为：
所述荧光探针是由八元瓜环和盐酸小檗碱制成；所述荧光探针的具体制备方法包括如下步骤：
(1)将八元瓜环加水溶解，得溶液A；步骤(1)所述溶液A的浓度为1.0×10-4mol/L；
(2)将盐酸小檗碱加水溶解，得溶液B；步骤(2)所述溶液B的浓度为1.0×10-3mol/L；所述溶液A和溶液B混合时，混合液中八元瓜环与盐酸小檗碱的摩尔比为1:2；
所述的荧光探针检测L-苯丙氨酸的方法，是将所述荧光探针加水稀释，得探针标准溶液，然后向探针标准溶液中加入待测物水溶液，静置5-20s后以固定激发波长350nm进行荧光发射光谱测定，并绘制激发出的该激光波长处的荧光强度的变化曲线；根据变化曲线计算荧光探针溶液中加入待测物水溶液前后分别对应521.04nm下的荧光发射光谱强度变化，即可对L-苯丙氨酸进行检测；所述探针标准溶液的浓度为2.0×10-5mol/L。
2.根据权利要求1所述的基于八元瓜环荧光探针检测L-苯丙氨酸的应用，其特征在于：当加入所述待测物水溶液前后探针标准溶液在521.04nm下的荧光发射光谱强度增强，则表明待测物水溶液中含有L-苯丙氨酸，否则不含。
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