Disclosure of Invention
The invention aims to provide a full-automatic sample injection blood cell analysis and measurement method and device, which can automatically distinguish venous blood from peripheral blood and automatically perform sample injection detection on the venous blood and the peripheral blood.
To achieve the object of the present invention, an embodiment of the present invention provides a fully automatic sample injection blood cell analysis and measurement method, including the following steps:
determining the loading condition of the test tube on the test tube rack in the process of transversely feeding the sample introduction loading platform to the test tube rack;
determining a test tube type of a test tube loaded in the test tube rack in the case that it is determined that the test tube rack is loaded with test tubes, wherein the test tube type includes a venous blood test tube and a peripheral blood test tube;
determining a corresponding mixing mode according to the test tube type, and mixing the blood in the test tube by adopting the determined mixing mode;
when the test tube rack is fed to a sample sucking position, determining a descending height according to the determined test tube type, and executing a descending action corresponding to the determined descending height by a sampling needle and sucking a sample;
and analyzing and measuring the absorbed sample, after the analysis and measurement are completed, feeding the test tube rack to an unloading position, unloading and withdrawing to an unloading platform, and outputting an analysis and measurement result of the absorbed sample.
Optionally, in one embodiment, the bottom thickness of the peripheral blood tube is greater than the bottom thickness of the venous blood tube, and the bottom of the peripheral blood tube is light permeable.
Optionally, in an embodiment, the peripheral blood test tube includes an outer tube and an inner tube, a bottom of the inner tube is located in a middle region of the outer tube, and a lower region of the outer tube is light-permeable.
Optionally, in an embodiment, the determining, according to the test tube type, a corresponding blending manner, and performing blending operation on the blood in the test tube by using the determined blending manner further includes:
and under the condition that the test tube type is a peripheral blood test tube, carrying out blending operation on the blood sample in the test tube by using a peripheral blood test tube blending mechanism.
Optionally, in an embodiment, the mixing the blood sample in the test tube by using a peripheral blood test tube mixing mechanism further includes:
will the test tube is placed in the test tube storehouse that link up of tip blood test tube mixing mechanism, makes the bottom of test tube holds in the palm the butt with the test tube of tip blood test tube mixing mechanism.
Optionally, in an embodiment, the placing the test tube in a through test tube bin of a peripheral blood test tube mixing mechanism, and after the bottom of the test tube abuts against a test tube holder of the peripheral blood test tube mixing mechanism, the placing further includes:
the bottom of test tube with tip blood test tube mixing mechanism be under the condition of conduit saddle butt, the brushless motor of tip blood test tube mixing mechanism drives the eccentric block and rotates, and then drives the test tube support of test tube butt produces vibrations, causes to link up tip blood test tube rotation swing and vibrations in the test tube storehouse to realize the mixing of the interior blood sample of test tube.
Optionally, in an embodiment, the determining, according to the test tube type, a corresponding blending manner, and performing blending operation on the blood in the test tube by using the determined blending manner further includes:
under the condition that the test tube type is the venous blood test tube, the test tube is got and is moved to the test-tube rack top from the test-tube rack middle clamp to the test tube clamping jaw, implements to make a round trip to reverse and carries out the mixing to the blood sample.
Optionally, in an embodiment, in a case that the test tube type is a venous blood test tube, a rotation speed parameter corresponding to the venous blood test tube is determined, and a brushless motor of the peripheral blood test tube blending mechanism performs a blending operation on a blood sample in the test tube by using the determined rotation speed parameter.
In addition, the embodiment of the invention also provides a full-automatic sample injection blood cell analysis and measurement device, which comprises a control unit, and the device also comprises a sample analysis device, a venous blood test tube blending mechanism, a peripheral blood test tube blending mechanism, an unloading and exiting platform mechanism, a sampling needle, a sample injection loading platform and a test tube detection unit, wherein the sample analysis device, the venous blood test tube blending mechanism, the peripheral blood test tube blending mechanism, the unloading and exiting platform mechanism, the sampling needle, the sample injection loading platform and the test tube detection unit are connected with the control unit and are controlled by the;
the sample introduction loading platform is used for placing a test tube rack and feeding the test tube rack to the sample analysis mechanism so that the sample analysis mechanism can carry out sample analysis and measurement on a blood sample in a test tube on the test tube rack;
the test tube detection unit is used for detecting the loading condition and the type of the test tube on the test tube rack;
the sampling needle is used for executing descending action corresponding to the descending height according to the descending height determined by the control unit and sucking a sample to be used for the sample analysis device to analyze and measure the sample;
the venous blood test tube blending mechanism and/or the peripheral blood test tube blending mechanism are/is used for blending blood samples in test tubes on the test tube rack;
the unloading and withdrawing platform mechanism is used for unloading and withdrawing the test tube rack.
Optionally, in an embodiment, the test tube detection unit is a correlation optical coupler, and may be disposed at any one of the blending positions on the sample introduction loading platform or the working positions before the blending position.
Optionally, in an embodiment, a test tube claw is arranged on the venous blood test tube blending mechanism, and the test tube claw is used for clamping a test tube from a test tube rack and moving the test tube to the upper part of the test tube rack, so as to implement back-and-forth inversion to blend a blood sample.
Optionally, in an embodiment, the peripheral blood test tube mixing mechanism includes a feeding mechanism, at least three horizontal supports, a flexible column, a through test tube bin, a test tube holder with an inner concave curved surface, a brushless motor, and an eccentric block
Optionally, in an embodiment, the brushless motor is located on a second horizontal support, the test tube carrier is located on the second horizontal support, and the through test tube bin is located on a third horizontal support.
Optionally, in one embodiment, one end of the flexible column is connected to the first horizontal bracket, and the other end is connected to the second horizontal bracket.
The method and the device of the embodiment of the invention have the following beneficial effects:
the invention provides a full-automatic sample injection blood cell analysis and measurement method and a device, which realize a simpler and more convenient peripheral blood whole blood detection operation mode, firstly, the method does not need external pre-dilution of diluent, secondly, the method has no strict quantitative requirement influence on a measured sample, and only needs to meet the requirement of minimum test dosage, like a venous blood measurement mode. Meanwhile, the method and the device can automatically identify the test tube types of the venous blood sample collection test tube and the peripheral blood sample collection test tube through the detection unit, implement a corresponding blood mixing mode according to the test tubes, control the sampling needle to automatically sample and analyze according to different test tube types, and finally output an analysis and measurement result. The method and the device provided by the embodiment of the invention have high automation degree, release the labor intensity of clinical personnel and improve the sampling and detecting efficiency of the blood sample.
In order to make the aforementioned and other objects, features and advantages of the present invention comprehensible, preferred embodiments accompanied with figures are described in detail below.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is apparent that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. The components of embodiments of the present invention generally described and illustrated in the figures herein may be arranged and designed in a wide variety of different configurations. Thus, the following detailed description of the embodiments of the present invention, presented in the figures, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. All other embodiments, which can be obtained by a person skilled in the art without making creative efforts based on the embodiments of the present invention, belong to the protection scope of the present invention.
In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "inner", "outer", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience in describing the present invention and simplifying the description, but do not indicate or imply that the referred device or element must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
In the description of the present invention, it should be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.
In this embodiment, a full-automatic sample injection blood cell analysis and measurement method and device are provided, which can automatically distinguish venous blood from peripheral blood and perform automatic sample injection detection on the venous blood and the peripheral blood.
Specifically, in an embodiment, as shown in fig. 1, a full-automatic sample injection blood cell analysis and measurement apparatus is provided, which includes a control unit 100, and the apparatus further includes a sample analysis apparatus 1001 connected to the control unit 100 and controlled by the control unit to perform corresponding operations by sending control instructions, a venous blood test tube blending mechanism 200, a peripheral blood test tube blending mechanism 300, an unloading and exiting platform mechanism 2, a sampling needle 7, a sample injection loading platform 6, and a test tube detection unit 1002;
wherein,
the sample introduction loading platform is used for placing a test tube rack and feeding the test tube rack to the sample analysis device so that the sample analysis device can carry out sample analysis and measurement on a blood sample in a test tube on the test tube rack;
the test tube detection unit is used for detecting the loading condition and the type of the test tube on the test tube rack;
the sampling needle is used for executing descending action corresponding to the descending height according to the descending height determined by the control unit and sucking a sample to be used for the sample analysis device to analyze and measure the sample;
the venous blood test tube blending mechanism and/or the peripheral blood test tube blending mechanism are/is used for blending blood samples in test tubes on the test tube rack;
the unloading and withdrawing platform mechanism is used for unloading and withdrawing the test tube rack.
Specifically, the test tube detection unit is configured to detect a loading condition of a test tube in the test tube rack, and detect a type of the test tube corresponding to the loaded test tube. The test tube detecting element detects and judges blood sample test tube type in the test-tube rack, and blood sample test tube is vein blood test tube or tip blood test tube in the test-tube rack promptly, according to test tube detecting element's testing result, the control unit corresponds the test tube of the different test tube types of discernment on to the test-tube rack and carries out vein blood test tube mixing mechanism mixing or tip blood test tube mixing mechanism mixing, to two kinds of different test tube types of vein blood test tube and tip blood test tube, the different degree of depth that the control unit control sampling needle descends absorbs the blood sample in the test tube, for the sample analysis appearance carries out sample analysis and measurement.
For example, the full autosampler blood cell analysis measuring device may be based on a physical device as shown in fig. 2.
The embodiment of the invention also provides a full-automatic sampling blood cell analysis and measurement method, as shown in fig. 3, the method comprises the following steps:
step S1: and determining the loading condition of the test tube on the test tube rack in the process of transversely feeding the sample loading platform to the test tube rack.
Whether the test tube rack is loaded with test tubes or not is detected by the test tube detecting unit, and this step is performed with the specimen sample loading platform feeding the test tube rack laterally. That is, the subsequent steps are performed only in the case where the test tubes are loaded on the test tube rack, so as to avoid unnecessary procedure waste.
In a specific embodiment, the test tube detecting unit is a pair of light coupling, and under the condition that the test tube rack transversely feeds through the test tube and detects the correlation opto-coupler, if the test tube has or not detects the correlation opto-coupler and produces the signal of sheltering from, judge that there is the test tube to exist in the test tube rack, otherwise, confirm not having the loading in the test tube rack to have the test tube.
Step S2: in the case where it is determined that the test tube is loaded on the test tube rack, determining a test tube type of the test tube loaded in the test tube rack, wherein the test tube type includes a venous blood test tube and a peripheral blood test tube.
When a test tube is loaded on a test tube rack, a blood sample in the loaded test tube needs to be analyzed. In this embodiment, there may be two types of test tubes in the test tube rack, one is a normal venous blood test tube and one is a special peripheral blood test tube, and different test tubes correspond to different subsequent operation steps. Therefore, the tube type needs to be distinguished. Specifically, the test tube type of the test tube loaded in the test tube rack is detected by the test tube detecting unit, wherein the test tube type includes a venous blood test tube and a peripheral blood test tube.
In an alternative embodiment, the peripheral blood tube has a bottom thickness greater than the bottom thickness of the venous blood tube, wherein the bottom thickness of the tube is the distance between the bottom of the exterior of the tube and the bottom of the tube lumen (the bottom of the tube lumen that can contain the blood sample). For example, the bottom of the test tube lumen of the peripheral blood test tube is located in the middle of the test tube, and the thickness of the tube bottom is half of the overall height of the test tube, while the thickness of the tube bottom of the venous blood test tube is the general thickness of the test tube wall, and is negligible compared with the overall height of the test tube. Generally, the test tube is made of glass or PP, that is, the portion corresponding to the thickness of the tube bottom is light permeable, and is light permeable no matter whether a blood sample is filled in the inner cavity of the test tube.
In the case of containing a blood sample, the lower region of the tube corresponding to the bottom thickness of the peripheral blood tube is light-permeable, and in the case of containing a blood sample, the lower region of the tube is not light-permeable because the bottom thickness of the venous blood tube is small and the light-permeable region of the bottom is negligible.
In another alternative embodiment, as shown in fig. 4, the peripheral blood test tube comprises an outer tube and an inner tube, wherein the bottom of the inner tube is located in the middle region of the outer tube, and the lower region of the outer tube is light permeable.
As shown in fig. 4, the peripheral blood test tube includes a tube body 11 and a tube cap 12; the whole or the middle part of the tube cap 12 is made of rubber so that a sampling needle can penetrate through the tube cap; the tube body 11 comprises an inner tube body and an outer tube body, wherein the bottom of the inner tube body is provided with a tapered round bottom so as to facilitate the depth of the sampling needle immersed into the blood sample; the outer tube body is a skirt edge 111 formed by downwards extending a certain length of a straight edge above the taper of the bottom of the inner tube body along the height direction of the tube body, so that a tip blood test tube can be placed in the test tube rack and can be clamped and placed by the test tube clamping jaws without changing the test tube rack; the skirt 111 is in a transparent or semitransparent state near the bottom area, and does not shield the detection light of the vacuum blood collection tube. Specifically, as shown in fig. 5 and 6, fig. 5 and 6 show a case where a venous blood test tube and a peripheral blood test tube are loaded on a test tube rack, wherein, as shown in fig. 5, a blood sample in the venous blood test tube is assigned to a reference number 011, a blood sample in the peripheral blood test tube is assigned to a reference number 021, and as shown in fig. 6, a venous blood test tube is assigned to a reference number 010, and a peripheral blood test tube is assigned to a reference number 020 are loaded on the test tube rack 5.
In another alternative embodiment, the cuvette detecting unit is a photoelectric sensor, which may be a correlation optical coupler, for example, and determines the detection of the bottom or lower area of the cuvette by the correlation optical coupler, and determines the type of the cuvette according to the photoelectric information received by the correlation optical coupler. In this embodiment, the test tube detecting unit for determining the test tube type may be disposed at any working position on the sample loading platform before the blending position, that is, the test tube type is detected before the blending operation.
Optionally, test tube detecting element chooses for use the correlation opto-coupler, as shown in fig. 7, wherein, whether the first test tube detecting element who loads the test tube on the test-tube rack is correlation opto-coupler 8, set up in on the venous blood test tube mixing mechanism, the second test tube detecting element who detects the test tube type is correlation opto-coupler 10, set up in on the tip blood test tube mixing mechanism.
That is, when the test tube rack transversely feeds to a preset working position, if the test tube detection correlation optical coupler is not shielded, the test tube detection unit judges that the test tube is a micro peripheral blood test tube; if the test tube detects that the correlation opto-coupler is sheltered from, judge as the venous blood test tube. Specifically, the method of the embodiment of the invention realizes the detection of two test tube types, namely a venous blood test tube and a peripheral blood test tube, by newly adding a high-low level of a correlation optical coupler arranged in a blood sample area at the bottom of the test tube and judging whether the test tube is in a test tube state or not; the peripheral blood test tube is in a transparent or semitransparent state corresponding to the tube body area of the newly added correlation optical coupler.
Step S3: and determining a corresponding mixing mode according to the test tube type, and mixing the blood in the test tube by adopting the determined mixing mode.
In this embodiment, the venous blood test tube and the peripheral blood test tube correspond to different blending modes, and the amount and the position of the blood sample are different, so that it is necessary to determine the blending mode corresponding to the test tube type to perform blending operation on the blood in the test tube, so as to improve the effective rate and the accuracy of subsequent sample suction and analysis and measurement.
Specifically, in the case where the test tube type is a venous blood test tube, a normal mixing operation corresponding to the venous blood test tube is adopted to perform the mixing operation. For example, the test tube gripper 9 picks up the test tube from the tube rack and moves it above the rack, and the mixing of the blood sample is performed by reversing the direction of the test tube.
When the test tube type is a peripheral blood test tube, it is necessary to perform a kneading operation using a kneading operation corresponding to the peripheral blood type. Specifically, a peripheral blood tube mixing mechanism is used for mixing the blood sample in the test tube. Tip blood test tube mixing mechanism is inside to be provided with and to link up test tube storehouse, test tube support, brushless motor, eccentric block, at the in-process that carries out the mixing operation, and control will the test tube is placed in the test tube storehouse that link up of tip blood test tube mixing mechanism, makes the bottom of test tube and the test tube support butt of tip blood test tube mixing mechanism to make the effect of the mixing operation in later stage best. The bottom of test tube with tip blood test tube mixing mechanism be under the condition of conduit saddle butt, the brushless motor of tip blood test tube mixing mechanism drives the eccentric block and rotates, and then drives the test tube support of test tube butt produces vibrations, causes to link up tip blood test tube rotation swing and vibrations in the test tube storehouse to realize the mixing of the interior blood sample of test tube.
In another alternative embodiment, in order to save space of the device, the mixing mechanisms for mixing the venous blood test tube and the peripheral blood can be provided as the same mechanism, for example, both the peripheral blood test tube mixing mechanisms. Specifically, in this embodiment, when the test tube type is a venous blood test tube, a rotation speed parameter corresponding to the venous blood test tube is determined, and the brushless motor of the peripheral blood test tube blending mechanism performs blending operation on a blood sample in the test tube by using the determined rotation speed parameter. That is, different blending parameters (for example, the rotation speed parameter of the brushless motor) corresponding to the peripheral blood test tube and the venous blood test tube are adopted for different test tube types to perform blending operation.
As shown in fig. 8, a venous blood tube mixing mechanism is shown at 200, a peripheral blood tube mixing mechanism is shown at 300, and a sample sucking and sampling needle mechanism including a sampling needle 7 is shown at 400.
Step S4: and when the test tube rack is fed to the sample sucking position, determining the descending height according to the determined test tube type, and executing the descending action corresponding to the determined descending height by the sampling needle and sucking the sample.
As mentioned above, the height of the blood sample in different types of test tubes varies due to the different types of test tubes (bottom height) and the different amount of blood in the test tubes, which results in different heights for the sampling needle to take samples, and if the same height is used, the sample may be taken or not enough in some cases. Therefore, in the embodiment, in the case that the test tube rack is fed to the sample sucking position, it is necessary to determine the corresponding position of the sampling needle in the process of sucking the sample, i.e. the corresponding descending height, according to the type of the test tube, then control the sampling needle to perform the descending action corresponding to the determined descending height, and then perform the operation of sucking the sample, so as to improve the success rate of sucking the sample and improve the effectiveness of subsequent sample analysis.
Step S5: and analyzing and measuring the absorbed sample, after the analysis and measurement are completed, feeding the test tube rack to an unloading position, unloading and withdrawing to an unloading platform, and outputting an analysis and measurement result of the absorbed sample.
In the step, the sample analysis mechanism carries out analysis measurement on the sample sucked by the sampling needle, and outputs a corresponding analysis measurement result for use after the analysis measurement is finished; and, after the analysis and measurement is completed, the test tube on the test tube rack no longer needs to be operated, in which case the test tube on the test tube rack needs to be unloaded, that is, the test tube rack is controlled to be fed to the unloading position to be withdrawn to the unloading platform to complete the process of automatic sample feeding and analysis and measurement.
In the embodiment of the invention, specifically, after the test tube state and type are identified, for example, when a peripheral blood test tube is identified, the test tube clamping jaw on the venous blood test tube mixing mechanism is horizontally fed to a proper position from the ready position to clamp the peripheral blood test tube; then move upward along the test tube axial direction to a proper height, ensure to lift the test tube and draw off from the test tube rack and the space between the lifted test tube bottom and the test tube rack top surface allows the horizontal feeding insertion of the peripheral blood test tube mixing mechanism. That is to say, be provided with the test tube claw on the venous blood test tube mixing mechanism, the test tube claw is used for the test tube on the centre gripping test-tube rack in order to remove the test tube. In the embodiment of the invention, after the test tube state and type are identified, for example, when a peripheral blood test tube is identified, the test tube clamping jaw 9 on the venous blood test tube mixing mechanism firstly carries out horizontal feeding to a proper position in the test tube direction from the ready position, and clamps the peripheral blood test tube; then moves upward along the axial direction of the test tube to a proper height, ensures that the lifted test tube is drawn away from the test tube rack 5 and is lifted to the space between the bottoms of the test tubes and the top surface of the test tube rack 5 to allow the horizontal feeding insertion of the peripheral blood test tube mixing mechanism.
Specifically, the specific procedure of the above-described mixing operation of the peripheral blood test tube includes steps S31 to S33 shown in fig. 9, and before the steps of the corresponding mixing operation are described, the peripheral blood test tube mixing mechanism for performing the mixing operation is first described in detail:
as shown in fig. 10, the peripheral blood test tube mixing mechanism 300: comprises a feeding mechanism, a third horizontal bracket 21, a second horizontal bracket 19, a brushless motor 24 and an eccentric block 26; a test tube bin 22 is arranged on the third horizontal bracket 19, and a test tube holder with an inwards concave curved surface is arranged on the second horizontal bracket; the test tube holder 23 is used for supporting a peripheral blood test tube, and the test tube bin 22 is used for limiting the test tube on the test tube holder 23; the brushless motor 24 of the peripheral blood test tube mixing mechanism drives the eccentric block 26 to rotate around the motor shaft of the brushless motor 24, and further drives the second horizontal support and the test tube holder to swing in a rotary mode.
Further, the feeding mechanism according to the embodiment of the present invention preferably includes: the horizontal feeding linear motor 13 is characterized in that a motor shaft of the horizontal feeding linear motor 13 is fixed to a base 14 of the L-shaped peripheral blood test tube blending mechanism along the horizontal direction, a horizontal linear guide rail 15 is fixed on the base 14, a fixing block 16 is fixed on a sliding block corresponding to a sliding piece on the linear guide rail 15, and a moving push plate 17 is connected with the fixing block 16 and a sliding screw nut of the linear motor 13 respectively.
Further, the peripheral blood test tube uniformly mixing mechanism 300 further comprises a first horizontal support 18, the first horizontal support 18 is fixed on the fixing block 16, a flexible rubber shock absorption column 25 of a second horizontal support 19 is connected and fixed above the first horizontal support 18, and a rubber test tube support 23 with an inwards concave curved surface is fixed on the second horizontal support 19; the support rod 20 is fixed above the first horizontal bracket 18, and the height of the support rod 20 is higher than that of the second horizontal bracket 19, so that a clearance should be avoided in the passing area of the second horizontal bracket 19; the third horizontal bracket 21 is fixed on the upper end of the support rod 20 and a through test tube bin 22 is fixed on the support rod, the brushless motor 24 is fixed above the second horizontal bracket 19, the eccentric block 26 is fixed on the output shaft end of the brushless motor 24, the movable push plate 17 is fixed with a horizontal moving position shielding optical coupler blocking sheet 27, and a corresponding horizontal position detection optical coupler 28 is fixed on the base 14.
The peripheral blood test tube mixing mechanism 300 according to the embodiment of the present invention has the following working principle: under the drive of the horizontal feeding linear motor 13, a sliding nut of the linear motor 13 drives the moving push plate 17, the optical coupling blocking piece 27 and the fixed block 16 to perform horizontal movement along the guiding direction of the horizontal linear guide rail 15, and a first horizontal support 18, a second horizontal support 19, a support rod 20, a third horizontal support 21, a through test tube bin 22, a test tube holder 23, a brushless motor 24, a flexible rubber shock absorption column 25 and an eccentric block 26 which are arranged on the fixed block 16 in a linkage mode synchronously and horizontally move. Under the drive of the brushless motor 24, an eccentric block 26 fixed on the output shaft end of the brushless motor 24 rotates around the motor shaft; since the second horizontal bracket 19 for fixing the brushless motor 24 is connected and fixed above the first horizontal bracket 18 through the flexible rubber shock-absorbing column 25, and the rotating shaft hole of the eccentric block 26 is not on the center of gravity thereof, the eccentric block 26 drives the second horizontal bracket 19 to swing and vibrate in a rotary manner when rotating.
Specifically, the specific process of the above mixing operation of the peripheral blood test tube is as follows:
step S31: controlling the test tube clamping jaw to clamp the peripheral blood test tube to ascend;
step S32: controlling the tip blood test tube blending mechanism to feed to a blending position, clamping the tip blood test tube by the test tube clamping jaw to descend, placing the tip blood test tube in a through test tube bin of the tip blood test tube blending mechanism, abutting the bottom of the tip blood test tube with a test tube holder of the tip blood test tube blending mechanism, and executing horizontal feeding by the test tube clamping jaw to move to a horizontal ready position far away from the direction of the test tube rack;
step S33: the brushless motor of tip blood test tube mixing mechanism drives the eccentric block and rotates, and then drives and link up the test tube and hold in the palm and produce the rotation swing, cause put with the test tube holds in the palm the blood specimen in the tip blood vessel of butt by the mixing.
Optionally, after the blending operation corresponding to the peripheral blood tube corresponding to the steps S31-S33 is performed, the method further includes the following steps:
step S34: controlling the test tube clamping jaw to clamp the uniformly mixed peripheral blood test tube to rise so that the peripheral blood test tube is far away from the through test tube bin;
step S35: the peripheral blood test tube mixing mechanism moves away from the test tube rack;
step S36: and controlling the test tube clamping jaws to clamp the uniformly mixed peripheral blood test tube to descend, and putting the uniformly mixed peripheral blood test tube back into the test tube rack.
Optionally, the step S33 further includes: a brushless motor of the peripheral blood test tube mixing mechanism is driven for a certain time, and the eccentric block rotates around a motor shaft to further drive the second horizontal support to swing in a rotary mode; the test tube is fixed on the test tube support with the concave curved surface on the second horizontal bracket, and the bottom of the test tube support swings along with the second horizontal bracket in a rotary mode. In the embodiment of the invention, the test tube falls on the rubber test tube holder 23 with the concave curved surface fixed on the second horizontal bracket 19, the bottom of the test tube swings and vibrates in a rotary manner along with the second horizontal bracket 19, and the test tube passes through the test tube bin 22 and is relatively static to restrict the tube body of the test tube, and the bottom of the test tube swings relatively greatly; therefore, the blood sample in the test tube is mixed uniformly under the multiple influences of centrifugal swing and vibration, and blood cells are prevented from being broken.
Optionally, the mixing operation corresponding to the venous blood test tube includes: the test tube clamping jaw lifts corresponding test tube to the test tube rack top from the test tube rack, and after implementing the back and forth reversal mixing, the test tube is put back to the test tube rack again and withdraws from the test tube clamping jaw.
Alternatively, in step S35, when the test tube is identified as a venous blood test tube, the sampling needle is lowered to a depth set according to the test tube type corresponding to the venous blood test tube; when the peripheral blood test tube is identified, the descending depth of the sampling needle is set according to the test tube type of the peripheral blood test tube; the falling depth of the sampling needle arranged in the venous blood test tube is larger than that of the sampling needle arranged in the peripheral blood test tube.
Optionally, the full-automatic blood cell analysis and measurement device that advances still includes the automatic counting module, and it is used for triggering automatic analysis and the count operation to blood sample, and for the convenience of starting, the automatic counting module is provided with automatic counting button 1 as shown in fig. 2, presses automatic counting button 1 and can start full-automatic sample analysis and measurement under the whole blood mode. And, pressing the automatic counting button 1 corresponds to informing the control unit that an analysis has been performed, and this counting operation can be used for counting the number of times of analyzing test tubes or for checking the number of analyzing test tubes.
As can be seen from the above description, the method and apparatus for implementing the embodiments of the present invention have the following advantages:
the invention provides a full-automatic sample injection blood cell analysis and measurement method and a device, which realize a simpler and more convenient peripheral blood whole blood detection operation mode, firstly, the method does not need external pre-dilution of diluent, secondly, the method has no strict quantitative requirement influence on a measured sample, and only needs to meet the requirement of minimum test dosage, like a venous blood measurement mode. Meanwhile, the method and the device can automatically identify the test tube types of the venous blood sample collection test tube and the peripheral blood sample collection test tube through the detection unit, implement a corresponding blood mixing mode according to the test tubes, control the sampling needle to automatically sample and analyze according to different test tube types, and finally output an analysis and measurement result. The method and the device provided by the embodiment of the invention have high automation degree, release the labor intensity of clinical personnel and improve the sampling and detecting efficiency of the blood sample.
While the invention has been described in the specification and drawings with reference to specific embodiments, it will be understood by those skilled in the art that various changes may be made and equivalents may be substituted for elements thereof without departing from the scope of the invention as defined in the claims. Furthermore, the combination and arrangement of features, elements and/or functions between specific embodiments herein is clearly apparent and thus, in light of this disclosure, one skilled in the art will appreciate that features, elements and/or functions of an embodiment may be incorporated into another embodiment as appropriate, unless described otherwise, above. In addition, many modifications may be made to adapt a particular situation or material to the teachings of the invention without departing from the essential scope thereof. Therefore, it is intended that the invention not be limited to the particular embodiment illustrated by the drawings and described in the specification as the best mode presently contemplated for carrying out this invention, but that the invention will include all embodiments falling within the scope of the foregoing description and the appended claims.