JP2005127896A - Device and method for sampling blood sample, or the like - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a sampling device of blood, or the like capable of appropriately and smoothly performing sampling of each sample and the cleaning of a sampling tube, after the sampling, by a common sample sampling device to hermetic- and open-type sample containers, and to provide a sampling method. <P>SOLUTION: The sampling device comprises a member 10 for upper cleaning and a member 14 for lower cleaning that can be moved in the vertical directions with respect to the member 10 for upper part cleaning. The member 10 for upper part cleaning has a passage hole 10a for inserting a sampling tube 16 and a cleaning liquid discharge hole 10b. At the upper opening edge section of the passage hole 10a, an O-ring 11 is provided so as to surround and retain the sampling tube 16. A piercing needle 12 communicates with and is fixed at the lower opening edge section of the passage hole 10a. The member 14 for the lower cleaning part has a passage hole 14a for inserting the piercing needle 12 and a cleaning liquid supplying hole 14b. At the upper opening edge section of the passage hole 14a, an O-ring 15 is provided so as to surround and retain the piercing needle 12. <P>COPYRIGHT: (C)2005,JPO&NCIPI

Description

  The present invention relates to a method and apparatus for sampling a sample of blood or the like collected by a blood collection tube or the like and cleaning a sampling tube in a blood test apparatus or the like, and in particular, a sealed sample container and an open sample container are commonly used. The present invention relates to a sample sampling apparatus and a sampling method that can be appropriately and smoothly performed by the sample sampling apparatus.

  Today, an automatic blood cell counter is known as a typical blood test apparatus. As shown in FIG. 7, for example, the automatic blood cell counter includes a blood quantification / dilution processor A, a blood cell detector B, and a counter processor C. In the automatic blood cell counter configured as described above, blood containing a certain amount of anticoagulant from the blood collection tube A1 is appropriately sampled into the dilution containers A3 and A4 via the sampling tube A2. Next, the blood sampled in the dilution containers A3 and A4 is transferred to the reaction chamber and the counting chamber together with an appropriate amount of diluent and hemolytic agent A5, and in the blood cell detector B, the red blood cell / platelet B1, white blood cell B2, and hemoglobin concentration B3 is detected. In this way, the measurement signals detected by the blood cell detector B are respectively subjected to arithmetic processing C1, C2, C3 by the counting processor C, and the measurement results are output and displayed by the display means C4. Yes.

  However, in the conventional blood cell counter configured as described above, for example, when the blood of the blood collection tube is sucked (sampled) into the dilution detector via the suction tube (sampling tube), the suction tube (sampling tube) If the sample blood adheres to the outer peripheral portion, this blood is mixed into the blood to be diluted, and the dilution factor varies. Therefore, in such a case, there is a problem that a significant measurement error occurs in the measurement result of the blood cell count.

  From this point of view, the applicant can first remove the blood adhering to the outer peripheral portion of the suction tube (sampling tube) without using a dedicated cleaning solution, and can accurately remove blood cells. Was developed and a patent was obtained (see Patent Document 1). That is, the blood cell counter described in Patent Document 1 is a blood cell counter including a suction tube that sucks sample blood and discharges it into a counting container that counts the number of blood cells in the blood. And a cleaning means for cleaning and removing the blood adhering to the outer periphery of the suction tube with a cleaning liquid after the sample blood has been sucked, and a cleaning drainage liquid for cleaning the counting container after blood cell counting for the suction tube It has a configuration in which liquid feeding means for feeding liquid to the washing means is provided as a cleaning liquid.

  Further, regarding a pipette device in an automatic analyzer configured to suck a required amount of liquid and discharge the liquid at another position, the pipette is bent even from inside a container with a rubber stopper. There has been proposed a pipette device having a simple configuration that can easily suck a liquid without damaging it and that does not require a separate liquid level detecting means (see Patent Document 2). That is, the pipette device described in Patent Document 2 includes a fixed outer cylinder and an inner cylinder that can be moved up and down and is housed in the fixed outer cylinder, and the inner cylinder and the fixed outer cylinder. The gap portion is configured to be supplied with cleaning water.

  Furthermore, as a sample collection device that can automatically collect blood from a blood collection tube in the past, when collecting a sample (blood) inside a sample container (collection tube) by piercing the nozzle into a rubber stopper, a collection nozzle is provided. A sampling device that prevents direct contact with the rubber stopper has been proposed (see Patent Document 3). That is, in the sample collecting device described in Patent Document 3, the nozzle portion is composed of an inner nozzle and an outer nozzle, and when puncturing the rubber stopper, the puncturing is performed with the inner nozzle stored in the outer nozzle. After the puncture, the sample is collected by lowering the tip of the inner nozzle from the tip opening of the outer nozzle, and after the sample is collected, the nozzle is cleaned using a cleaning tank. is there.

Japanese Patent Publication No. 7-119759 Japanese Utility Model Publication 4-898 Japanese Patent Laid-Open No. 7-83938

However, an open type and a sealed type are conventionally used as blood collection tubes for blood collection in blood tests, blood analysis, and the like. Therefore, when sampling (sampling) samples from these blood collection tubes, different sampling mechanisms are used.
That is, when sampling from a sealed blood collection tube, it is necessary to use a sampling mechanism provided with a sampling tube whose tip is formed in a needle shape. On the other hand, when sampling from an open-type blood collection tube, the sampling mechanism including the sampling tube is dangerous, and its use is avoided. In addition, in order to perform sampling from a sealed blood collection tube with high accuracy, it is necessary to open the blood collection tube to the atmosphere in advance, and a mechanism for that purpose is required. Therefore, it is difficult to share the sampling mechanism. It was.

  In general, in the case of a sealed blood collection tube, when an automated blood test apparatus or blood analysis apparatus is applied, it can be set so as to be integrated with a sampling mechanism for sampling. On the other hand, in the case of an open type blood collection tube, since manual operation is required for sampling, the sampling tube needs to be exposed to the outside. For this reason, when the sampling tube is cleaned after each sampling, it is difficult to make the cleaning operation common because of the difference in operation of the sampling mechanism.

  Therefore, the present inventors have made various studies in order to obtain a method and an apparatus that can perform appropriate and smooth sampling of the sealed-type blood collection tube and the open-type blood collection tube using a common sampling device, respectively. As a result of repeated trial production, the upper cleaning member and a lower cleaning member movable up and down with respect to the upper cleaning member are provided. The upper cleaning member includes a through-hole and a cleaning liquid drain hole through which the sampling pipe is inserted. An O-ring is provided at the upper opening edge of the through hole so as to surround and hold the sampling tube, and a piercing needle is connected and fixed to the lower opening edge of the through hole for cleaning the lower part. The member has a through-hole through which the pierce needle is inserted and a cleaning liquid water supply hole, and an upper opening edge of the through-hole is provided with an O-ring so as to surround and hold the pierce needle. Open the device It was successful.

  That is, in the sample sampling device for blood or the like, a support arm is provided at one end of the upper cleaning member, and this support arm is connected to a drive motor fixedly arranged at a predetermined position at one end of a feed screw that is rotationally driven. A part of a holder support arm that supports a holder for holding and holding a blood collection tube is screwed to the feed screw, and the holder is moved up and down by the action of the feed screw. The support arm is provided at one end of the lower cleaning member, and the support arm is inserted into the guide shaft having one end coupled and fixed to the support arm of the upper cleaning member together with the holder support arm, A return spring consisting of a compression coil spring is mounted between the support arms of the upper cleaning member and the lower cleaning member of the guide shaft, and is lowered below the holder support arm. By adopting a configuration in which the starter switch is provided on the extended holder lower support plate, the blood sampling operation and the sampling tube cleaning operation using the closed-type blood collection tube and the open-type blood collection tube described above can be performed appropriately and smoothly. I found out that I can achieve it. In addition, even in the sample sampling device apparatus in which the upper cleaning member can be moved up and down and the lower cleaning member is fixed, the blood sampling operation and the sampling tube cleaning using the sealed and open blood collection tubes are similarly performed. Operation is possible.

  Accordingly, an object of the present invention is to perform proper and smooth sampling of a sealed sample container and an open sample container by using a common sample sampling apparatus, respectively, for sample sampling and subsequent cleaning of the sampling tube. An object of the present invention is to provide a sampling device for blood and the like and a sampling method.

  In order to achieve the above object, a blood sampling device according to claim 1 of the present invention comprises an upper cleaning member and a lower cleaning member that can move up and down relative to the upper cleaning member. The upper cleaning member has a through-hole through which the sampling pipe is inserted and a cleaning liquid drain hole, and an O-ring is provided at the upper opening edge of the through-hole so as to surround and hold the sampling pipe. A piercing needle is connected and fixed to the lower opening edge of the hole, and the lower cleaning member has a through hole through which the piercing needle is inserted and a cleaning liquid water supply hole. An O-ring is provided so as to surround and hold the piercing needle.

  According to a second aspect of the present invention, there is provided a sampler for blood or the like, wherein a support arm is provided at one end of the upper cleaning member, and the support arm is rotationally driven by being coupled to a drive motor fixedly arranged at a predetermined position. A part of a holder support arm that supports a holder for storing and holding a sample container via a hinge is screwed and attached to the one end of the feed screw. It is configured to move up and down by the action, and a support arm is provided at one end of the lower cleaning member, and this support arm is connected to the support arm of the upper cleaning member at a guide shaft and fixed together with the holder support arm. A return spring comprising a compression coil spring is mounted between the support arms of the upper cleaning member and the lower cleaning member of the guide shaft, and is attached to the holder support arm. Characterized in that a starting switch to the holder lower support plate extending downwardly.

  According to a third aspect of the present invention, there is provided an apparatus for sampling a blood sample or the like according to the present invention, wherein the upper cleaning member and the lower cleaning member are configured and arranged such that one of them can be moved up and down relatively. A through hole for inserting the sampling pipe and a cleaning liquid drain hole, and an upper opening edge of the through hole is provided with an O-ring so as to surround and hold the sampling pipe, and a lower opening edge of the through hole The piercing needle is connected and fixed, and the lower cleaning member has a through hole through which the piercing needle is inserted and a cleaning liquid water supply hole. The upper opening edge of the through hole surrounds and holds the piercing needle. Thus, an O-ring is provided.

According to a fourth aspect of the present invention, there is provided a method for sampling blood or the like, wherein an upper cleaning member having a piercing needle inserted through a sampling tube so as to be movable up and down is fixedly disposed, and the piercing is provided below the upper cleaning member. A sample sampling device for blood or the like, comprising a lower cleaning member that moves up and down by inserting a needle, and a holder for storing and holding a sample container that can be moved up and down by a drive motor below the lower cleaning member In
(1) The lower cleaning member is fixed to the cleaning standby position surrounding the tip of the piercing needle of the upper cleaning member, and the drive motor is rotated to fix the holder to the lower portion of the lower cleaning member, and the sampling tube is Descending to the sampling position and waiting;
(2) setting an open sample container to the sampling tube and inserting the sampling tube into the sample container;
(3) Inserting the sampling tube up to the sampling position in the sample container, operating the start switch provided on the lower support plate of the holder, and sampling the sample;
(4) A cleaning step of raising the sampling tube to the cleaning position with respect to the upper cleaning member after completion of predetermined sampling, and supplying a cleaning liquid from the lower cleaning member to the upper cleaning member through the inside of the piercing needle;
(5) a step of dispensing the sampled sample by further raising the sampling tube after completion of the predetermined cleaning;
It is characterized by comprising.

According to a fifth aspect of the present invention, there is provided a method for sampling a sample of blood or the like, wherein an upper cleaning member having a piercing needle that is vertically inserted into a sampling tube and the piercing needle is inserted below the upper cleaning member. The lower cleaning member is configured so that one of the lower cleaning members can be moved up and down relatively, and a holder for storing and holding the sample container is provided so that the lower cleaning member can be moved up and down by a drive motor. In a sample sampling device such as blood
According to the type of the sample container, it is set to select an operation for inserting the piercing needle and an up / down movement of the holder by a driving motor.

  According to a sample sampling apparatus for blood or the like according to claims 1 and 2 of the present invention, the apparatus includes an upper cleaning member and a lower cleaning member that can move up and down with respect to the upper cleaning member. Has a through-hole through which the sampling pipe is inserted and a cleaning liquid drain hole, and an O-ring is provided at an upper opening edge of the through-hole so as to surround and hold the sampling pipe, and a lower opening edge of the through-hole A pierce needle is connected and fixed to the portion, and the lower cleaning member has a through hole through which the pierce needle is inserted and a cleaning liquid supply hole, and the upper opening edge of the through hole surrounds the pierce needle. By adopting a configuration in which an O-ring is provided so as to hold, the sampling of the sample and the subsequent cleaning of the sampling tube are performed in a common sample sampling device for the sealed sample container and the open sample container, respectively. Yo , It can be carried out properly and smoothly.

  According to the sample sampling apparatus described in claim 3 of the present invention, even if the design of the sample sampling mechanism is slightly changed, the operation is appropriately selected according to the type of the sealed sample container or the open sample container. The sampling of the sample and the subsequent cleaning of the sampling tube can be performed appropriately and smoothly by a common sample sampling apparatus.

  According to a sample sampling method for blood or the like according to claim 4 of the present invention, the sample sampling apparatus having the above configuration is applied, and the lower cleaning member surrounds the tip of the piercing needle of the upper cleaning member. In the case of using an open-type sample container, the drive motor is driven to rotate and the holder is fixed to the lower part of the lower cleaning member, and the sampling tube is lowered to the sampling position to stand by. From the sampling of the sample, the subsequent cleaning of the sampling tube can be achieved smoothly.

  Furthermore, according to the sample sampling method for blood or the like according to claim 5 of the present invention, the operation is appropriately performed according to the type of the sealed sample container or the open sample container even if the design of the sample sampling mechanism is slightly changed. By selecting, it is possible to smoothly achieve the subsequent sampling tube cleaning from the sampling of the sample.

  Next, embodiments of a sample sampling device for blood and the like according to the present invention and a sampling method will be described in detail with reference to the accompanying drawings.

  FIG. 1 is a blood sampling device showing an embodiment of a sample sampling device such as blood according to the present invention. That is, the blood sampling apparatus of this embodiment includes an upper cleaning member 10 and a lower cleaning member 14 that can move up and down with respect to the upper cleaning member 10. The upper cleaning member 10 has a through hole 10a through which the sampling pipe 16 is inserted and a cleaning liquid drain hole 10b, and an O-ring is provided at the upper opening edge of the through hole 10a so as to surround and hold the sampling pipe 16. 11 is provided, and a piercing needle 12 is connected and fixed to the lower opening edge of the through hole 10a. The lower cleaning member 14 has a through hole 14a through which the piercing needle 12 is inserted and a cleaning liquid water supply hole 14b. The upper opening edge of the through hole 14a surrounds and holds the piercing needle 12. Is provided with an O-ring 15 (see FIG. 1).

  However, in the blood sampling apparatus of the present embodiment, as shown in FIG. 1, a support arm 30 is provided at one end of the upper cleaning member 10, and this support arm 30 is fixedly arranged at a predetermined position. It is pivotally supported at one end of a feed screw 32 which is coupled to 31 and rotates. A part of a holder support arm 42 that supports a holder 40 for storing and holding the sealed blood collection tube 20 </ b> A via a hinge 41 is screwed to the feed screw 32. That is, the holder 40 is configured to move up and down by the action of the feed screw 32. The holder 40 is provided with an insertion hole 40a through which the sampling tube 16 is inserted. Further, a support arm 34 is provided at one end of the lower cleaning member 14, and the support arm 34 is attached to a guide shaft 36 whose one end is coupled and fixed to the support arm 30 of the upper cleaning member 10. 42 is inserted. Then, a return spring 35 made of a compression coil spring is mounted between the support arm 30 and the support arm 34 of the guide shaft 36. Furthermore, a starter switch 46 that is operated when an open type blood collection tube 20B described later is used is provided on the holder lower support plate 44 that extends downward with respect to the holder support arm 42. Reference numeral 50 denotes an apparatus main body case, and is set so that the start switch 46 is positioned inside the apparatus main body when the holder 40 is in the lowered position.

  The blood sampling apparatus of the present embodiment configured as described above is configured so that the blood sampling operation and the sampling tube are performed by the sampling tube 16 when the sealed blood collection tube 20A is used as in the second embodiment shown in FIG. 16 cleaning operations can be performed appropriately and smoothly. Further, the blood sampling apparatus of the present embodiment performs setting of the holder 40 as shown in FIG. 4 and changes the operation of the sampling tube 16 to change the operation of the sampling tube 16 as in Embodiment 3 shown in FIG. When using the open-type blood collection tube 20B, the blood sampling operation and the sampling tube 16 washing operation by the sampling tube 16 can be performed appropriately and smoothly.

  Regarding the blood sampling operation using the sampling tube 16 and the washing operation of the sampling tube 16 in the case of targeting the sealed blood collection tube 20A using the blood sampling apparatus having the above-described configuration, FIG. A description will be given together with a schematic configuration shown in FIG.

2A, first, the lower cleaning member 14 is set to a cleaning standby position in which the tip of the piercing needle 12 of the upper cleaning member 10 is surrounded by an O-ring 15. At this time, the sampling tube 16 stands by at a position above the upper cleaning member 10. Then, the holder 40 is opened (STEP-31).
In FIG. 2B, after the sealed blood collection tube 20A is set in the holder 40, the holder 40 is closed (STEP-32).
2 (c), the drive motor 31 is rotationally driven to raise the holder 40 by the action of the feed screw 32, the blood collection tube 20A is brought into contact with the lower portion of the lower cleaning member 14, and the lower cleaning member 14 is further moved. While raising against the elasticity of the return spring 35 to contact the lower part of the upper cleaning member 10, the tip of the piercing needle 12 is pierced through the rubber stopper 21 of the blood collection tube 20A (STEP-33).
In FIG. 2 (d), the sampling tube 16 is lowered and inserted to the sampling position in the blood collection tube 20A to sample blood (STEP-34).
In FIG. 5 (e), the sampling tube 16 is raised after completion of predetermined sampling, the drive motor 31 is driven to rotate and the holder is lowered by the action of the feed screw 32, and the elastic force of the return spring 35 is used for lower cleaning. The member 14 is lowered to the cleaning standby position (STEP-35).
In FIG. 2F, the sampling tube 16 is raised to the cleaning standby position with respect to the upper cleaning member 10, and the upper cleaning is performed from the cleaning liquid supply hole 14 b of the lower cleaning member 14 through the inside of the piercing needle 12. Cleaning is performed by supplying a cleaning liquid to the cleaning liquid drain hole 10b of the member 10 under a negative pressure condition (STEP-36). In this case, the blood adhering to the outer periphery of the sampling tube 16 is scraped and collected inside the through hole 10a of the upper cleaning member 10 and the piercing needle 12 by the action of the O-ring 11 when rising. When the lower cleaning member 14 descends, the attached blood is scraped up inside the through hole 14a of the lower cleaning member 14 by the action of the O-ring 15, and is smoothly drained and washed to the outside by the flow of the cleaning liquid. .
In (g) of FIG. 2, the sampling tube 16 is further raised after a predetermined washing operation to dispense the sampled blood, and the holder is further lowered by the action of the feed screw 32 by driving the drive motor 31 to rotate. Then, the holder 40 for storing and holding the blood collection tube 20A is opened (STEP-37).
In this way, the state shown in FIG. 2A is entered, and the standby state for the next sampling operation of the blood collection tube 20A is established.

  FIG. 5A shows a blood sampling operation using the sampling tube 16 and a washing operation of the sampling tube 16 when the blood sampling apparatus having the above-described configuration is used for the open-type blood collection tube 20B. It demonstrates, referring also the flowchart figure which shows the operation | movement process of FIG. 6 with the schematic structure shown to (e).

5A, first, the lower cleaning member 14 is fixed at a cleaning standby position in which the tip of the piercing needle 12 of the upper cleaning member 10 is surrounded by an O-ring 15. At this time, the drive motor 31 is rotationally driven, the holder 40 is fixed to the lower portion of the lower cleaning member 14 by the action of the feed screw 32, and the sampling tube 16 is lowered to the sampling position and waits (STEP-41).
In FIG. 5B, an open-type blood collection tube 20B is set in the sampling tube 16, and the sampling tube 16 is inserted into the blood collection tube 20B (STEP-42).
In FIG. 8C, the sampling tube 16 is inserted to the sampling position in the blood collection tube 20B, and the start switch 46 provided on the lower support plate 44 of the holder 40 is operated to sample blood (STEP-43). .
In FIG. 5D, the sampling pipe 16 is raised to the cleaning standby position with respect to the upper cleaning member 10 after completion of the predetermined sampling, and the cleaning liquid water supply hole 14b of the lower cleaning member 14 is passed through the inside of the piercing needle 12. Then, the cleaning liquid is supplied to the cleaning liquid drain hole 10b of the upper cleaning member 10 under a negative pressure condition to perform cleaning (STEP-44). In this case, the blood adhering to the outer periphery of the sampling tube 16 is scraped and collected inside the through hole 10a of the upper cleaning member 10 and the piercing needle 12 by the action of the O-ring 11, and smoothly flows due to the circulation of the cleaning liquid. The waste water is washed outside.
In FIG. 5 (e), after completion of the predetermined cleaning, the sampling tube 16 is further raised to dispense the sampled sample (STEP-45).
In this way, the state shown in FIG. 5A is obtained, and a standby state for the sampling operation of the next blood collection tube 20B is established.

  The preferred embodiment of the present invention has been described above. However, the present invention is not limited to the blood sampling apparatus of the above-described embodiment. For example, the upper cleaning member can be moved up and down and the lower cleaning member is fixed. The sampling operation of the sample and the cleaning operation of the sampling tube by the sealed sample container and the open sample container are possible, and it can be effectively applied as a sampling device for various samples other than blood, Many other design changes can be made without departing from the spirit of the present invention.

It is a schematic block diagram which shows one Example of sample sampling devices, such as blood, concerning the present invention. (A)-(g) is explanatory drawing which shows the process from the sampling start to the end in the case of sampling blood from a sealed blood collection tube using the sample sampling device such as blood shown in FIG. . It is a flowchart figure which shows the operation | movement process of the sampling method in sample sampling devices, such as blood, which concerns on this invention shown in FIG. It is a schematic block diagram which shows the setting state of the sample sampling device in the case of sampling blood from an open type blood collection tube by the sample sampling device such as blood according to the present invention shown in FIG. (A)-(e) is explanatory drawing which shows the process from the sampling start to the end in the case of sampling blood from an open type blood collection tube in the setting state of sample sampling devices, such as blood shown in FIG. . It is a flowchart figure which shows the operation | movement process of the sampling method in sample sampling devices, such as blood, which concerns on this invention shown in FIG. 1 is a schematic block connection diagram showing a system configuration of an automatic blood cell counter.

Explanation of symbols

DESCRIPTION OF SYMBOLS 10 Upper cleaning member 10a Through hole 10b Cleaning liquid drain hole 11 O ring 12 Pierce needle 14 Lower cleaning member 14a Through hole 14b Cleaning liquid water supply hole 15 O ring 16 Sampling tube 20A Sealed blood collection tube 20B Open type blood collection tube 30 Support Arm 31 Drive motor 32 Feed screw 34 Support arm 35 Return spring 36 Guide shaft 40 Holder 40a Sampling tube insertion hole 41 Hinge 42 Holder support arm 44 Holder lower plate 46 Start switch 50 Device body case

Claims (5)

  An upper cleaning member and a lower cleaning member movable up and down with respect to the upper cleaning member, the upper cleaning member having a through hole through which a sampling pipe is inserted and a cleaning liquid drain hole, An O-ring is provided at the upper opening edge of the opening so as to surround and hold the sampling tube. A piercing needle is connected and fixed to the lower opening edge of the through hole, and the lower cleaning member is provided with the piercing needle. A sample sampling apparatus for blood or the like, characterized by having a through-hole to be inserted and a cleaning liquid supply hole, and an O-ring provided at the upper opening edge of the through-hole so as to surround and hold the piercing needle.   A support arm is provided at one end of the upper cleaning member, and the support arm is pivotally supported at one end of a feed screw that is coupled to a drive motor fixedly arranged at a predetermined position and rotationally driven. A holder support arm for supporting a holder for storing and holding the holder through a hinge is screwed and mounted, and the holder is moved up and down by the action of a feed screw, and is supported by one end of a lower cleaning member An arm is provided, and this support arm is inserted together with the holder support arm into a guide shaft whose one end is coupled and fixed to the support arm of the upper cleaning member, and the upper cleaning member and the lower cleaning member of the guide shaft are inserted. A return spring comprising a compression coil spring is mounted between each support arm, and a starter switch is provided on a holder lower support plate that extends downward with respect to the holder support arm. Sampling system, such as blood according to claim 1, wherein the door.   The upper cleaning member and the lower cleaning member are configured and arranged so that one of them can be moved up and down relatively, and the upper cleaning member has a through hole through which the sampling pipe is inserted and a cleaning liquid drain hole, An O-ring is provided at the upper opening edge of the through hole so as to surround and hold the sampling tube, and a piercing needle is connected and fixed to the lower opening edge of the through hole. A sample of blood or the like having a through hole for inserting a needle and a cleaning liquid water supply hole, and an O-ring provided at the upper opening edge of the through hole so as to surround and hold the pierced needle apparatus. An upper cleaning member having a piercing needle that is inserted through the sampling tube so as to be movable up and down is fixedly disposed, and a lower cleaning member that is vertically moved by inserting the piercing needle is disposed below the upper cleaning member, In a sample sampling device such as blood, which comprises a holder that can be moved up and down by a drive motor below the lower washing member and stores and holds the sample container,
(1) The lower cleaning member is fixed to the cleaning standby position surrounding the tip of the piercing needle of the upper cleaning member, and the drive motor is rotated to fix the holder to the lower portion of the lower cleaning member, and the sampling tube is Descending to the sampling position and waiting;
(2) setting an open sample container to the sampling tube and inserting the sampling tube into the sample container;
(3) Inserting the sampling tube up to the sampling position in the sample container, operating the start switch provided on the lower support plate of the holder, and sampling the sample;
(4) A cleaning step of raising the sampling tube to the cleaning position with respect to the upper cleaning member after completion of predetermined sampling, and supplying a cleaning liquid from the lower cleaning member to the upper cleaning member through the inside of the piercing needle;
(5) a step of dispensing the sampled sample by further raising the sampling tube after completion of the predetermined cleaning;
A method for sampling a sample of blood or the like, comprising: One of the upper cleaning member having a piercing needle that is inserted through the sampling tube so as to be movable up and down and the lower cleaning member through which the piercing needle is inserted below the upper cleaning member can be relatively moved up and down. In a sample sampling apparatus for blood or the like comprising a holder for storing and holding a sample container that can be moved up and down by a drive motor with respect to the lower cleaning member.
A sample sampling method for blood or the like, which is set to select an operation for inserting the piercing needle and an up / down movement of the holder by a drive motor according to the type of the sample container.

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