CN107328950B - Automatic sample injection system for lifting and cleaning sample tube in cell analysis - Google Patents
Automatic sample injection system for lifting and cleaning sample tube in cell analysis Download PDFInfo
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- CN107328950B CN107328950B CN201710724844.4A CN201710724844A CN107328950B CN 107328950 B CN107328950 B CN 107328950B CN 201710724844 A CN201710724844 A CN 201710724844A CN 107328950 B CN107328950 B CN 107328950B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N35/1004—Cleaning sample transfer devices
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
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Abstract
The invention discloses an automatic sample injection system for lifting and cleaning a sample tube in cell analysis, which comprises a sampling needle and a swab, wherein the sampling needle is fixedly arranged on a sampling needle support, the sampling needle support is fixedly connected with a substrate, a swab sliding mechanism and a sample tube lifting mechanism are arranged on the substrate, the swab is connected with the swab sliding mechanism, the sample tube is placed on the sample tube lifting mechanism, the sample tube lifting mechanism is lifted upwards under the action of a driving mechanism, in the lifting process, an opening part at the upper end of the sample tube is tightly matched with the bottom of the swab to form a seal and jack the swab, the sample tube lifting mechanism and the swab sliding mechanism synchronously move, and the sample tube and the swab always keep a relative sealing state in the tightly matched synchronous movement process. The invention can simultaneously complete the relative movement and position control of the swab and the sampling needle and the suction and transfer of the sample in the sample tube, and can complete the full-automatic sample injection, cleaning and separation of the cell sample tube.
Description
Technical Field
The invention belongs to the technical field of blood cell analysis equipment, and particularly relates to an automatic sample injection system for lifting and cleaning a sample tube in cell analysis.
Background
In medical in-vitro diagnostic analyzers, such as blood cell analyzers and flow cytometers, flow focusing is generally used to constrain cells in a sample into single cell fluid, and pulse signals (including optical scattering, fluorescent signals or electrical impedance signals) generated when the cells sequentially flow through a quartz flow cell or a microwell are measured, and information statistics and analysis are performed on the pulse signals of different amplitudes and widths by an electronic system. The diagnosis results of the cell analyzers are important links for diagnosing and analyzing the disease conditions of patients, and can directly guide the development of subsequent medical behaviors.
According to the difference of different blood cells in physical and biochemical properties, the method can be used for distinguishing and counting the cell types in different systems based on the Coulter principle and flow cytometry by adopting cell staining, light scattering, electrical impedance and other modes, and provides application information such as five classification of blood cells, immune typing, tumor ploidy analysis, cytokines and the like. When the cell analyzers perform diagnostic analysis, a certain amount of samples such as fluorescent marked blood or cells and the like are firstly required to be sucked from a blood sampling tube or a test tube through a sample loading device, and the sucked samples are injected into an analysis tank or a flow chamber through a sample injection capillary or a sample injection needle and are sequentially detected and identified by the instrument. How to automatically transfer the sample after the user has placed the sample tube (including the blood collection tube or test tube) is therefore a primary goal of the sample loading device.
Meanwhile, after the samples such as blood, cells and the like are sucked from the test tube, residues are left on the inner surface and the outer surface of the sampling needle and the sample introduction channel, and before the next sample is collected and analyzed, the residual samples or cells need to be cleaned to avoid cross contamination to the detection of the next sample. The general principle at present is to use a cleaning solution flushing and collecting device (hereinafter referred to as "swab"), such as the swab structure disclosed in US patent No. 5,408,891 and chinese patent application nos. 200610021359.2 and 201210222699.7, which describe the detailed design of each cavity, liquid inlet and liquid outlet of the swab, but do not give any structure or scheme for the relative up-and-down movement of the swab, sampling needle and test tube.
In addition, due to the consideration of cleanliness and safety of medical instruments, no liquid splashing in the sample loading process is required to be ensured, and the loading mechanical structure is not damaged to medical staff. In the chinese patent with application number 201210222699.7, a sample loading needle structure with a baffle ring is proposed to avoid the pollution of the blood bubbles in the test tube to the swab; however, in the sample injection process in the document, the sampling needle moves up and down rapidly, so that a great potential safety hazard exists in puncture, and no sample injection structure is disclosed.
Therefore, how to simultaneously complete the relative movement and the up-down position control of the swab and the sampling needle, complete the suction and transfer of the sample in the sample tube, ensure no liquid splash and no potential safety hazard is a technical problem to be solved by the person in the field. If a full-automatic cell sample injection, cleaning and separation system can be designed, the residual samples on the inner surface and the outer surface of the sampling needle are cleaned, and the characteristics of low cost and convenient use are ensured through reasonable arrangement, so that the flow cytometer, the blood cell analyzer and other analysis instruments have higher market competitiveness.
Disclosure of Invention
The invention aims at: aiming at the problems, the automatic sample injection system for lifting and cleaning the sample tube in the cell analysis can finish the sample injection, cleaning and separation of the full-automatic cell sample tube and clean the residual sample on the inner surface and the outer surface of the sampling needle.
The technical scheme of the invention is realized as follows: an automatic sampling system for lifting and cleaning a sample tube in cell analysis comprises a sampling needle and a swab, and is characterized in that: the sampling needle is fixedly arranged on a sampling needle support, the sampling needle support is fixedly connected with a substrate, a swab sliding mechanism and a sample tube lifting mechanism are arranged on the substrate, the swab is connected with the swab sliding mechanism, the sample tube is placed on the sample tube lifting mechanism, the sampling needle penetrates through the swab and stretches into the sample tube in the sampling process, and the sample tube lifting mechanism is lifted upwards under the action of a driving mechanism; in the lifting process, the opening part at the upper end of the sample tube is tightly matched with the bottom of the swab to form a seal and jack up the swab, the sample tube lifting mechanism and the swab sliding mechanism synchronously move, and the sample tube and the swab always keep a relative sealing state in the tightly matched synchronous movement process.
The automatic sample injection system for lifting and cleaning the sample tube in the cell analysis is characterized in that a tension spring is arranged on the swab sliding mechanism, one end of the tension spring is connected with the swab sliding mechanism, the other end of the tension spring is connected with the bottom of the substrate, and the tension spring is in a stretching state in the synchronous movement process of the sample tube and the swab.
The automatic sample injection system for lifting and cleaning the sample tube in the cell analysis is characterized in that a limiting groove is formed in the substrate along the sliding direction of a swab sliding mechanism, a sample loading baffle correspondingly matched with the limiting groove is arranged on the swab sliding mechanism, the upper end of a tension spring is connected with the sample loading baffle, the lower end of the tension spring is connected with a tension spring pin at the bottom of the substrate, when the swab sliding mechanism and the sample tube lifting mechanism are at the lowest positions, the sample tube and the swab are in a separation state, and the needle tip at the lower end of a sampling needle is arranged in the swab.
The automatic sample injection system for lifting and cleaning the sample tube in the cell analysis is characterized in that a fixed connecting rod is arranged between the swab sliding mechanism and the sample tube lifting mechanism, the lower end of the fixed connecting rod is fixedly connected with the sample tube lifting mechanism, a vertical chute is arranged on the fixed connecting rod, the vertical chute is correspondingly matched with a sliding protruding part on the swab sliding mechanism in a sliding manner, and when the sample tube lifting mechanism is at the lowest position, the sliding protruding part is positioned at the uppermost end of the vertical chute on the fixed connecting rod.
The invention relates to an automatic sample injection system for lifting and cleaning a sample tube in cell analysis, wherein a swab sliding mechanism comprises a swab sliding block assembly and a swab supporting frame, the swab supporting frame and a sample feeding baffle are respectively and fixedly connected to two end parts of the swab sliding block assembly, and a swab is fixedly connected to the end parts of the swab supporting frame; the sample tube lifting mechanism comprises a sample tube sliding block assembly and a sample tube lifting rod, the sample tube sliding block assembly is connected with the driving mechanism through a connecting component, the sample tube lifting rod and the connecting component are respectively and fixedly connected with two end parts of the sample tube sliding block assembly, the sample tube is placed at the end parts of the sample tube lifting rod, and the swab sliding block assembly and the sample tube sliding block assembly are respectively and slidably arranged on a linear guide rail module fixed on the substrate.
The automatic sample injection system for lifting and cleaning the sample tube in the cell analysis comprises a swab slide block, wherein the swab slide block consists of an upper guide rail slide block and an upper slide block, the upper guide rail slide block is slidably arranged on a linear guide rail module, the upper slide block is fixedly connected with the upper guide rail slide block, the upper end part of the upper slide block is fixedly connected with a swab support frame, the lower end part of the upper slide block is fixedly connected with a sample loading baffle, and a sliding protruding part is fixedly connected with the upper slide block; the sample tube sliding block is composed of a lower guide rail sliding block and a lower sliding block, the lower guide rail sliding block is slidably arranged on the linear guide rail module, the lower sliding block is fixedly connected with the lower guide rail sliding block, the upper end of the lower sliding block is fixedly connected with the connecting component, the lower end of the lower sliding block is fixedly connected with the sample tube lifting rod, and the lower end of the fixed connecting rod is fixedly connected with the lower sliding block.
The invention discloses an automatic sample injection system for lifting and cleaning a sample tube in cell analysis, which comprises a driving motor, a synchronous toothed belt, a driving wheel and a driven wheel, wherein the driving motor is fixedly connected to a substrate, the driving wheel is connected with a power output end of the driving motor, the driven wheel is rotatably arranged on the substrate, two ends of the synchronous toothed belt are wound on the driving wheel and the driven wheel, the synchronous toothed belt is connected with a lower slider of the sample tube lifting mechanism through a connecting component, and the sample tube lifting mechanism and the synchronous toothed belt synchronously move.
The invention discloses an automatic sample injection system for lifting and cleaning a sample tube in cell analysis, which comprises a sample loading belt gasket and a belt locking plate, wherein the sample loading belt gasket is fixedly connected to the upper end part of a lower sliding block, the sample loading belt gasket and the belt locking plate are oppositely arranged and clamp a synchronous toothed belt in the middle, photoelectric sensors are respectively arranged on the upper part and the lower part of a substrate, and the belt locking plate is matched with the corresponding photoelectric sensors and is used for judging the movement position of a sample tube lifting mechanism.
The invention relates to an automatic sample injection system for lifting and cleaning a sample tube in cell analysis, which comprises a swab body, wherein the center of the swab body is provided with a cavity, the cavity in the center of the swab body consists of an upper cavity, a lower cavity and a central through hole which is communicated with the upper cavity and the lower cavity, the diameters of the upper cavity and the lower cavity are larger than the diameter of the central through hole, namely the whole structure of the central cavity of the swab body is of a dumbbell-shaped structure with two large ends and a small middle part, the outer diameter of a sampling needle is smaller than the diameter of the central through hole, the upper cavity is communicated with a waste liquid tube, the lower cavity is communicated with a washing liquid tube, and the outer wall of the sampling needle is used for cleaning when the sampling needle passes through the central cavity of the swab body, and the needle tip of the sampling needle is positioned in the upper cavity and is used for cleaning the inner wall of the sampling needle in a backflushing mode.
The invention discloses an automatic sample injection system for lifting and cleaning a sample tube in cell analysis, which is characterized in that two ends of a swab body are respectively provided with an upper swab plug and a lower swab plug for sealing a central cavity of the swab, the upper swab plug is arranged on an upper swab round table formed on the upper end face of the swab body, the upper swab plug is matched with the upper swab round table to form an annular clamping part which is correspondingly matched with a fixing hole at the end part of a swab support frame of a swab sliding mechanism, the lower swab plug is arranged on the lower end face of the swab body and is provided with an inward concave arc structure, the inward concave arc of the lower swab plug is matched with an opening at the upper end of the sample tube to form a sealing structure, and central through holes for a sampling needle to pass through are respectively formed on the upper swab plug and the lower swab plug.
The invention aims to provide a sample injection system capable of automatically completing sample tube lifting and cleaning in cell analysis, which can complete full-automatic sample injection, cleaning and separation functions of a cell sample tube by utilizing a sampling needle fixing mode and through mutual matching and relative movement position control of a swab, the sample tube, a swab sliding mechanism and a sample tube lifting mechanism. The invention has simple integral structure, saves the sample needle cleaning time, improves the detection efficiency, completes the precise matching of two movable parts by only one driving mechanism, and ensures the characteristics of low cost and convenient use.
Drawings
Fig. 1 is a schematic structural view of the present invention.
Fig. 2 is a schematic diagram of a split structure of the present invention.
Fig. 3 is a schematic structural view of a substrate in the present invention.
Fig. 4 is a schematic view of the present invention in which the linear guide module and the driving mechanism are mounted on the base plate.
Fig. 5 is a schematic view of the structure of the swab sliding mechanism according to the present invention.
FIG. 6 is a schematic diagram of a sample tube lifting mechanism according to the present invention.
Fig. 7 is a schematic structural view of the swab sliding mechanism and the sample tube lifting mechanism in the present invention, which are respectively located at the upper and lower limit positions when the tension spring and the fixed connecting rod are not limited.
Fig. 8 and 9 are schematic views of the structure of the swab of the present invention.
The marks in the figure: 1 is a sampling needle, 2 is a swab, 3 is a sampling needle bracket, 4 is a substrate, 5 is a swab sliding mechanism, 6 is a sample tube lifting mechanism, 7 is a sample tube, 8 is a driving mechanism, 9 is a tension spring, 10 is a sample baffle, 11 is a fixed connecting rod, 12 is a sliding boss, 13 is a linear guide rail module, 14 is a sample feeding belt gasket, 15 is a belt locking plate, 16 is a pipe clamping sleeve, 21 is a swab body, 22 is an upper cavity, 23 is a lower cavity, 24 is a central through hole, 25 is a waste liquid tube, 26 is a washing liquid tube, 27 is a swab upper plug, 28 is a swab lower plug, 29 is a swab upper round table, 41 is a limit groove, 51 is a swab supporting frame, 52 is an upper guide rail slider, 53 is an upper slider, 61 is a sample tube lifting rod, 62 is a lower guide rail slider, 63 is a lower slider, 81 is a driving motor, 82 is a synchronous toothed belt, 83 is a driving wheel, 84 is a driven wheel, and 111 is a vertical chute.
Detailed Description
The present invention will be described in detail with reference to the accompanying drawings.
The present invention will be described in further detail with reference to the drawings and examples, in order to make the objects, technical solutions and advantages of the present invention more apparent. It should be understood that the specific embodiments described herein are for purposes of illustration only and are not intended to limit the scope of the invention.
As shown in fig. 1-6, an automatic sampling system for lifting and cleaning a sample tube in cell analysis comprises a sampling needle 1 and a swab 2, wherein the sampling needle 1 is fixedly arranged on a sampling needle support 3, the sampling needle support 3 is fixedly connected with a substrate 4, the swab 2 is used for sealing an upper port of the sample tube 7 in the lifting process of the sample tube 7 and cleaning the inner wall and the outer wall of the sampling needle 1 in the descending process, in the embodiment, the sampling needle 1 is used as a liquid channel for transferring a solution in the sample tube 7 to a flow chamber or an analysis tank, a pipe clamping sleeve 16 for controlling the opening or closing of a sample feeding pipeline is arranged on the sampling needle support 3, the sampling needle support 3 is used for fixing the sampling needle 1 and the pipe clamping sleeve 16, more specifically, the sampling needle 1 can be in an inverted L-shaped cylindrical hollow structure and comprises a vertical section and a horizontal section, the vertical section is used for inserting the bottom of the sample tube 7 for feeding, the length of the vertical section of the sampling needle 1 is slightly longer than the height of the sample tube 7, the sampling needle 1 can be ensured to be contacted with the bottom of the sample tube 7, the sampling needle 1 can be prevented from damaging the bottom of the sample tube 7, the sampling needle can be connected to the pipe through the pipe clamping sleeve 16 in the liquid channel 1 in the whole process, and the sample tube 1 can be prevented from being opened and the sample tube 1 can be effectively cleaned by a sample tube 1 in a static state by a sample feeding mode is in the process is kept in a sample tube 1.
The swab 2 is connected with the swab 2 bottom in a tight fit manner to form a seal and jack up the swab 2 in the lifting process, the sample tube lifting mechanism 6 and the swab sliding mechanism 5 are synchronously moved, the sample tube 7 and the swab 2 are always kept in a relative sealing state in the synchronous movement process of the tight fit, the tension spring 9 is in a stretching state in the synchronous movement process of the sample tube 7 and the swab 2, so that the sealing reliability of the sample tube and the swab can be ensured, and meanwhile, the downward acting force of the swab can be provided for the swab under the action of the tension spring when the swab is reset, and the swab can be also moved to a low power-off position under the action of the tension spring when the swab is reset. The sample tube is a holding device for a sample to be tested for cell analysis, is a cylindrical tubular structure with a hemispherical bottom, and comprises, but is not limited to, a medical vacuum blood collection tube, a 5mL sample tube for flow analysis, a 1-5 mL centrifuge tube for biological experiments and the like.
In this embodiment, the driving mechanism 8 includes a driving motor 81, a high torque synchronous toothed belt 82, a driving wheel 83 and a driven wheel 84, the driving motor adopts a stepping motor, the driving motor 81 is fixedly connected to the base plate 4, the driving wheel 83 is connected to a power output end of the driving motor 81, the driven wheel 84 is rotatably disposed on the base plate 4, two ends of the synchronous toothed belt 82 are wound on the driving wheel 83 and the driven wheel 84, the synchronous toothed belt 82 is connected to the lower slider 63 of the sample tube lifting mechanism 6 through a connection component, and the sample tube lifting mechanism 6 moves synchronously with the synchronous toothed belt 82. The stepping motor converts an electric pulse signal into angular displacement and drives the driving wheel to rotate, the wheel surface of the driving wheel is of a star-shaped tooth structure, and the driving wheel can form snap fit with the synchronous toothed belt, so that the synchronous toothed belt moves up and down and drives the sample tube lifting mechanism fixed to the synchronous toothed belt to synchronously move. The driven wheel is arranged on the base plate and right below the driven wheel, and more particularly, the driven wheel can comprise a driven wheel shaft, a driven wheel sleeve column, a flat washer and a fixing screw. The linear guide rail module is arranged in parallel with the synchronous toothed belt and is fixed to the side panel of the base plate for linear sliding of the sample tube lifting mechanism and the swab sliding mechanism.
As shown in fig. 3, the whole base plate 4 is in an L-shaped vertical page structure, the front panel is narrower and is used for fixing the automatic sampling system into an instrument, a connecting part is arranged in a square extending area at the top and is used for fixing the sampling needle bracket 3, the side panel is wider and is provided with a mounting hole for extending the output shaft of the driving motor 81 and fixing the driving motor 81, a square hole for fixing the photoelectric sensor is arranged, and a connecting hole for installing the linear guide rail module 13, wherein a limit groove 41 is arranged on the base plate 4 along the sliding direction of the swab sliding mechanism 5, the limit groove 41 is used for limiting the movement range of the swab, a loading baffle plate 10 correspondingly matched with the limit groove 41 is arranged on the swab sliding mechanism 5, the upper end of the tension spring 9 is connected with the loading baffle plate 10, the lower end of the tension spring 9 is connected with a tension spring pin at the bottom of the base plate 4, and the swab sliding mechanism is at the lowest position when the loading baffle plate moves to the lowest end of the limit groove on the base plate. The swab slide mechanism 5 is provided with a fixed connecting rod 11 between the sample tube slide mechanism 6, the lower end of the fixed connecting rod 11 is fixedly connected with the sample tube slide mechanism 6, a vertical chute 111 is arranged on the fixed connecting rod 11, the vertical chute 111 is correspondingly in sliding fit with a sliding protruding portion 12 on the swab slide mechanism 5, when the sample tube slide mechanism 6 is at the lowest position, the sliding protruding portion 12 is positioned at the uppermost position of the vertical chute 111 on the fixed connecting rod 11, namely, the sample tube slide mechanism is correspondingly hung on the swab slide mechanism through the fixed connecting rod. When the swab sliding mechanism 5 and the sample tube lifting mechanism 6 are both at the lowest positions, the sample tube 7 and the swab 2 are in a separation state so as to facilitate replacement of the sample tube, and at the moment, the needle tip part at the lower end of the sampling needle 1 is arranged in the swab 2, namely, the sampling needle adopts a fixed mode and the needle tip of the sampling needle is not exposed in the whole process, so that potential hazards of sampling to operators are effectively avoided.
The fixing connecting rod 11 is used for limiting the furthest position relation between the swab sliding mechanism 5 and the sample tube lifting mechanism 6, when no sample tube 7 is put in, the fixing connecting rod 11 is in a stretching stress state, and the sample tube lifting mechanism 6 is at the lowest position; when the sample tube 7 is put in and starts to be lifted, the fixed connecting rod 11 is in a contracted and unstressed state, and the distance between the swab sliding mechanism 5 and the sample tube lifting mechanism 6 is reduced. As shown in fig. 7, the farthest position and the mutual positional relationship to which the swab slide 5 and the sample tube lifting mechanism 6 can move are shown when the tension spring 9 and the fixed link 11 are not added to the system.
As shown in fig. 5, the swab sliding mechanism 5 includes a swab sliding block assembly and a swab supporting frame 51, the swab supporting frame 51 is used for fixing the swab 2, and is specifically a square arm-shaped structure, a larger end of the swab supporting frame is provided with a swab fixing hole, the whole hole is approximately circular, a semicircular lower edge is arranged at the most distal end, a protruding clip is arranged at the most distal end, the swab 2 can be pushed into the distal end from the proximal end to fix the swab 2 on the swab supporting frame 51, the swab supporting frame 51 and the loading baffle 10 are respectively and fixedly connected to two end parts of the swab sliding block assembly, the swab 2 is fixedly connected to the end parts of the swab supporting frame 51, wherein the swab sliding block assembly is composed of an upper guide rail sliding block 52 and an upper sliding block 53, the upper guide rail sliding block 52 is slidably arranged on a linear guide rail module 13, the upper sliding block 53 is fixedly connected with the upper guide rail sliding block 52, the upper end part of the upper sliding block 53 is fixedly connected with the loading baffle 10, the sliding protruding part 12 is fixedly connected to the upper sliding block 53, in this embodiment, the upper sliding block 53 is a short length L-shaped arm is equal to the length of the upper sliding block, and the upper sliding block is equal to the length of the upper sliding block 41.
As shown in fig. 6, the sample tube lifting mechanism 6 directly acts on the bottom of the sample tube 7, is an action unit of the whole automatic lifting and cleaning process, and comprises a sample tube slide block assembly and a sample tube lifting rod 61, wherein the sample tube lifting rod 61 is used for lifting the sample tube 7, and is of a square arm-shaped structure, a lifting circular ring is arranged at the smaller end of the sample tube lifting rod, the circular ring structure of the sample tube lifting rod is matched with the bottom of the sample tube 7 in a hemispherical manner, and is used for bearing and fixing the sample tube 7, the sample tube slide block assembly is connected with the driving mechanism 8 through a connecting component, the sample tube lifting rod 61 and the connecting component are respectively and fixedly connected at two ends of the sample tube slide block assembly, the sample tube 7 is placed at the end of the sample tube lifting rod 61, the sample tube slide block assembly is composed of a lower guide rail slide block 62 and a lower slide block 63, the lower guide rail slide block 62 is slidably arranged on the linear guide rail module 13, the lower slide block 63 is fixedly connected with the lower guide rail slide block 62, the upper end of the lower slide block 63 is fixedly connected with the connecting component, and the lower end of the lower slide block 63 is fixedly connected with the lower end of the lower slide block 11.
The connecting assembly comprises a sample loading belt gasket 14 and a belt locking plate 15, the sample loading belt gasket 14 is of an L-shaped square cylinder structure, a long arm of the connecting assembly is fixed to the vertical upper end portion of the lower sliding block, a short arm of the connecting assembly is mutually fixed with the belt locking plate 15, the belt locking plate 15 is of a T-shaped sheet structure, the sample loading belt gasket 14 and the belt locking plate 15 are oppositely arranged and clamp a synchronous toothed belt 82 in the middle, then a sample tube lifting mechanism 6 is fixed onto the synchronous toothed belt 82, the synchronous toothed belt 82 drives the sample tube lifting mechanism 6 to move in the automatic sample feeding process of the sample tube 7, U-shaped photoelectric sensors are respectively arranged on the upper portion and the lower portion of the base plate 4, the short arm of the belt locking plate 15 is matched with the corresponding photoelectric sensors to judge the movement position of the sample tube lifting mechanism 6, and the photoelectric sensors are mounted in square holes of the base plate.
As shown in fig. 8, the swab 2 is used for cleaning the inner wall and the outer wall of the sampling needle 1, and plays a role in guiding and limiting the movement path of the sample tube 7 in the loading process, the swab 2 includes a swab body 21 with a cavity in the center, the whole body is in a cylindrical structure, the cavity in the center of the swab body 21 is composed of an upper cavity 22, a lower cavity 23 and a central through hole 24 communicating the upper cavity 22 and the lower cavity 23, the diameters of the upper cavity 22 and the lower cavity 23 are larger than the diameter of the central through hole 24, that is, the whole body structure of the central cavity of the swab body 21 is in a dumbbell-shaped structure with two ends being larger and smaller in the middle, the outer diameter of the sampling needle 1 is slightly smaller than the diameter of the central through hole 24, in the embodiment, the diameters of the upper cavity and the lower cavity are far larger than the outer diameter of the sampling needle, such as 3-7 times, and the diameters of the central through hole are slightly larger than the outer diameter of the sampling needle, such as 0.25 mm; the upper cavity 22 is communicated with the waste liquid pipe 25, the lower cavity 23 is communicated with the washing liquid pipe 26, the waste liquid pipe 25 and the washing liquid pipe 26 can be steel pipes, and the inner diameter of the waste liquid pipe is larger than that of the washing liquid pipe.
When the sampling needle 1 passes through the central cavity of the swab body 21, the outer wall of the sampling needle 1 is cleaned, at this time, the cleaning liquid is sprayed to the outer surface of the sampling needle at a certain speed through the cleaning liquid pipe 26 under the action of positive pressure and forms a circulation in the lower cavity 23, meanwhile, the waste liquid pipe 25 is connected to negative pressure, and the cleaned waste liquid preferentially passes through the central through hole 24 and enters the upper cavity 22 under the action of pressure difference and is sucked into the waste liquid barrel through the waste liquid pipe; when the needle tip of the sampling needle 1 is located in the upper cavity 22, the inner wall of the sampling needle 1 is cleaned by a backflushing mode, and at this time, the cleaning liquid is backflushed to the upper cavity 22 through the inside of the sampling needle 1 under the action of forward pressure, the waste liquid pipe 25 is connected to negative pressure, and the waste liquid is preferentially sucked into the waste liquid barrel through the waste liquid pipe 25 under the action of pressure difference.
As shown in fig. 9, two ends of the swab body 21 are respectively provided with an upper swab plug 27 and a lower swab plug 28 for sealing the central cavity of the swab, the upper swab plug 27 is arranged on an upper swab round table 29 formed on the upper end surface of the swab body 21, the upper swab plug 27 and the upper swab round table 29 are matched to form an annular clamping part which is used for corresponding matching with a fixing hole at the end part of the swab support frame 51, the lower swab plug 28 is arranged on the lower end surface of the swab body 21 and is provided with an inward concave arc structure, the inward concave arc of the lower swab plug 28 is matched with an opening at the upper end of the sample tube 7 to form a sealing structure, central through holes for the passage of a sampling needle 1 are respectively formed in the upper swab plug 27 and the lower swab plug 28, the central through hole diameters of the upper swab plug and the lower swab plug are the same as and coaxial with the hole of the central through hole in the swab body, and the sampling needle can sequentially pass through the upper swab plug central through hole, the central through hole and the lower swab plug central through hole and move up and down.
The working principle of the invention is as follows:
the sample tube lifting and the sample needle cleaning can be specifically divided into two steps of a sample tube lifting process and a sample tube descending process, wherein the sample tube descending process can be further divided into two sections, and specifically comprises the following steps:
in the ascending process, the sample tube lifting mechanism 6 moves upwards, and the sample tube 7 is lifted to be sealed with the bottom of the swab 2, so that the swab 2 is jacked up, at the moment, the swab slider component and the sample tube slider component are not contacted, and the sample tube lifting rod 61, the sample tube 7 and the swab 2 form close fit. The swab 2 is moved to a high position with the sample tube lifting mechanism 6 until the sampling needle 1 is inserted into the bottom of the sample tube 7 and sample transfer is completed by the sampling needle 1.
In the first section of the descent process, the sample tube lifting mechanism 6 moves downward under the action of the stepping motor 81, and the swab sliding mechanism 5 is subjected to downward stretching force due to the tension spring 9 in a stretched state, so that the swab sliding mechanism 5 follows the downward movement. The swab 2 may begin to clean the outer wall of the sample needle 1 during the downward process, as described above. The first stage of descent is finished when the loading baffle 10 moves to the bottom of the limit groove 41, at this time, the swab sliding mechanism 5 moves to a stop position, at this time, the needle tip of the sampling needle 1 is still located in the upper cavity 22 of the swab 2, and at this time, the inner wall of the sampling needle 1 can be back-flushed.
In the second stage of the descent, the swab slide 5 has moved to its rest position and the sample tube lifting mechanism 6 continues to move downwards under the action of the stepper motor 81 until the fixed link 11 moves to its maximum position, i.e. the sliding boss 12 on the swab slide 5 is at the uppermost position of the vertical chute 111 on the fixed link. At this time, the sample tube lifting mechanism 6 moves to the lowest position, the distance between the swab sliding mechanism 5 and the sample tube lifting mechanism 6 is the largest, the sample tube 7 is separated from the lower end part of the swab 2, and a user or an instrument can take out the sample tube freely.
In the ascending process and the descending process of the sample tube 7, the sampling needle 1 at least penetrates through the center through hole of one swab 2, so that the sampling needle 1 is automatically aligned with the center through hole of the swab 2, the accuracy of the movement direction is ensured, and the sampling needle 1 is ensured to smoothly suck liquid from the test tube 7; on the other hand, the lower end opening of the swab lower plug 28 can prevent blood bubbles in the test tube 7 from entering the lower cavity 23 of the swab 2 to pollute the swab 2, and meanwhile, the swab lower plug also serves as a sample tube cover in the sample loading process, so that liquid splashing is effectively avoided.
The foregoing description of the preferred embodiments of the invention is not intended to be limiting, but rather is intended to cover all modifications, equivalents, and alternatives falling within the spirit and principles of the invention.
Claims (10)
1. An automatic sample injection system for lifting and cleaning a sample tube in cell analysis comprises a sampling needle (1) and a swab (2), and is characterized in that: the sampling needle (1) is fixedly arranged on the sampling needle support (3), the sampling needle support (3) is fixedly connected with the base plate (4), a swab sliding mechanism (5) and a sample tube lifting mechanism (6) are arranged on the base plate (4), the swab (2) is connected with the swab sliding mechanism (5), the sample tube (7) is placed on the sample tube lifting mechanism (6), the sampling needle (1) penetrates through the swab (2) and stretches into the bottom in the sample tube (7) in the sampling process, the sample tube lifting mechanism (6) is lifted upwards under the action of a driving mechanism (8), in the lifting process, an opening at the upper end of the sample tube (7) is tightly matched with the bottom of the swab (2) to form a seal and jack the swab (2), the sample tube lifting mechanism (6) and the swab sliding mechanism (5) synchronously move, and the sample tube (7) and the swab (2) always keep a relative sealing state in the tight-fit synchronous movement process;
the swab (2) comprises a swab body (21) with a cavity in the center, the cavity in the center of the swab body (21) is composed of an upper cavity (22), a lower cavity (23) and a central through hole (24) communicated with the upper cavity (22) and the lower cavity (23), the diameters of the upper cavity (22) and the lower cavity (23) are larger than the diameter of the central through hole (24), namely, the whole structure of the swab body (21) is of a dumbbell-shaped structure with large ends and small middle, when the needle tip of the sampling needle (1) passes through the central cavity of the swab body (21), the swab body is used for cleaning the outer wall of the sampling needle (1), and when the needle tip of the sampling needle (1) is located in the upper cavity (22), the swab body is used for cleaning the inner wall of the sampling needle (1) in a backflushing mode.
2. The automated sample injection system for lifting and cleaning of sample tubes in cell analysis of claim 1, wherein: the swab sliding mechanism (5) is provided with a tension spring (9), one end of the tension spring (9) is connected with the swab sliding mechanism (5), the other end of the tension spring is connected with the bottom of the base plate (4), and the tension spring (9) is in a stretching state in the synchronous movement process of the sample tube (7) and the swab (2).
3. The automated sample injection system for lifting and cleaning of sample tubes in cell analysis of claim 2, wherein: the sample tube is characterized in that a limiting groove (41) is formed in the base plate (4) along the sliding direction of the swab sliding mechanism (5), a sample loading baffle (10) corresponding to the limiting groove (41) is arranged on the swab sliding mechanism (5), the upper end of a tension spring (9) is connected with the sample loading baffle (10), the lower end of the tension spring is connected with a tension spring pin at the bottom of the base plate (4), and when the swab sliding mechanism (5) and the sample tube lifting mechanism (6) are both at the lowest positions, the sample tube (7) and the swab (2) are in a separation state, and the needle point at the lower end of the sampling needle (1) is arranged in the swab (2).
4. An automated sample injection system for lifting and cleaning a sample tube in a cell analysis according to claim 3, wherein: be provided with fixed connecting rod (11) between swab slide mechanism (5) and sample cell hoist mechanism (6), fixed connecting rod (11) lower extreme and sample cell hoist mechanism (6) fixed connection be provided with vertical spout (111) on fixed connecting rod (11), sliding fit is corresponding with slip bellying (12) on swab slide mechanism (5) in vertical spout (111), works as sample cell hoist mechanism (6) are in the minimum position, slip bellying (12) are located the uppermost position of vertical spout (111) on fixed connecting rod (11).
5. The automated sample injection system for lifting and washing of sample tubes in cell analysis of claim 4, wherein: the swab sliding mechanism (5) comprises a swab sliding block assembly and a swab supporting frame (51), the swab supporting frame (51) and the sample loading baffle (10) are respectively and fixedly connected to two end parts of the swab sliding block assembly, and the swab (2) is fixedly connected to the end parts of the swab supporting frame (51); sample tube lifting mechanism (6) are including sample tube slider constitution and sample tube lifting rod (61), sample tube slider constitution is connected with actuating mechanism (8) through coupling assembling, sample tube lifting rod (61) and coupling assembling are fixed connection respectively at the both ends that sample tube slider constitutes, sample tube (7) are placed in the tip of sample tube lifting rod (61), swab slider constitution and sample tube slider constitution set up respectively on linear guide module (13) on being fixed in base plate (4) slidingly.
6. The automated sample injection system for lifting and washing of sample tubes in cell analysis of claim 5, wherein: the swab slide block consists of an upper guide rail slide block (52) and an upper slide block (53), the upper guide rail slide block (52) is slidably arranged on the linear guide rail module (13), the upper slide block (53) is fixedly connected with the upper guide rail slide block (52), the upper end part of the upper slide block (53) is fixedly connected with the swab support frame (51), the lower end part of the upper slide block (53) is fixedly connected with the loading baffle (10), and the sliding protruding part (12) is fixedly connected with the upper slide block (53); the sample tube sliding block is composed of a lower guide rail sliding block (62) and a lower sliding block (63), the lower guide rail sliding block (62) is slidably arranged on the linear guide rail module (13), the lower sliding block (63) is fixedly connected with the lower guide rail sliding block (62), the upper end of the lower sliding block (63) is fixedly connected with the connecting component, the lower end of the lower sliding block (63) is fixedly connected with the sample tube lifting rod (61), and the lower end of the fixed connecting rod (11) is fixedly connected with the lower sliding block (63).
7. The automated sample injection system for lifting and cleaning of sample tubes in cell analysis of claim 6, wherein: the driving mechanism (8) comprises a driving motor (81), a synchronous toothed belt (82), a driving wheel (83) and a driven wheel (84), wherein the driving motor (81) is fixedly connected to the base plate (4), the driving wheel (83) is connected with a power output end of the driving motor (81), the driven wheel (84) is rotatably arranged on the base plate (4), two ends of the synchronous toothed belt (82) are wound on the driving wheel (83) and the driven wheel (84), the synchronous toothed belt (82) is connected with a lower sliding block (63) of the sample tube lifting mechanism (6) through a connecting component, and the sample tube lifting mechanism (6) and the synchronous toothed belt (82) synchronously move.
8. The automated sample injection system for lifting and washing of sample tubes in cell analysis of claim 7, wherein: the connecting assembly comprises a sample loading belt gasket (14) and a belt locking plate (15), wherein the sample loading belt gasket (14) is fixedly connected to the upper end part of the lower sliding block (63), the sample loading belt gasket (14) and the belt locking plate (15) are oppositely arranged and clamp a synchronous toothed belt (82) in the middle, photoelectric sensors are respectively arranged on the upper part and the lower part of the substrate (4), and the belt locking plate (15) is matched with the corresponding photoelectric sensors and is used for judging the movement position of the sample tube lifting mechanism (6).
9. The automated sample introduction system for lifting and washing of sample tubes in cell analysis of any one of claims 1 to 8, wherein: the outer diameter of the sampling needle (1) is smaller than the aperture of the central through hole (24), the upper cavity (22) is communicated with the waste liquid pipe (25), and the lower cavity (23) is communicated with the washing liquid pipe (26).
10. The automated sample injection system for lifting and cleaning of sample tubes in cell analysis of claim 9, wherein: the swab body (21) both ends are provided with respectively and are used for carrying out confined swab on stifled son (27) and swab under stifled son (28) to swab center cavity, on the swab on stifled son (27) set up on the swab on round platform (29) that swab body (21) up end formed, on the swab on stifled son (27) and swab on round platform (29) cooperation form be used for with swab slide mechanism (5) swab support frame (51) tip fixed orifices correspond complex cyclic annular joint portion, under the swab stifled son (28) set up the terminal surface under swab body (21) to be formed with indent arc structure, the indent arc of stifled son (28) under the swab cooperates with sample tube (7) upper end opening portion and forms seal structure on stifled son (27) and swab down be provided with the central through-hole that is used for sampling needle (1) to pass respectively.
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| CN201710724844.4A CN107328950B (en) | 2017-08-22 | 2017-08-22 | Automatic sample injection system for lifting and cleaning sample tube in cell analysis |
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| CN201710724844.4A CN107328950B (en) | 2017-08-22 | 2017-08-22 | Automatic sample injection system for lifting and cleaning sample tube in cell analysis |
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