CN115386476B

CN115386476B - Microbial colony counter

Info

Publication number: CN115386476B
Application number: CN202211243407.8A
Authority: CN
Inventors: 洪晓鸣; 丁宗宇; 王茜
Original assignee: Tianjin Haihe Mapping Technique Testing Co ltd
Current assignee: Tianjin Haihe Mapping Technique Testing Co ltd
Priority date: 2022-10-11
Filing date: 2022-10-11
Publication date: 2023-03-10
Anticipated expiration: 2042-10-11
Also published as: CN115386476A

Abstract

The invention discloses a microbial colony counter which comprises a light shield fixed above a chassis through a support frame, wherein the light shield is in a cylindrical structure, a sample dividing disc and a first mounting plate are coaxially arranged in the light shield from bottom to top in sequence, and the sample dividing disc can move towards the first mounting plate along the axial direction of the light shield; the sample dividing plate is provided with a plurality of sample grooves in an annular distribution mode, a sample bearing plate capable of moving in the vertical direction is arranged in each sample groove, sliding plates with the number being consistent with that of the sample grooves are distributed on the first mounting plate in an annular distribution mode, and each sliding plate is connected with the first mounting plate in a sliding mode along the radial direction of the first mounting plate. According to the microbial colony counter provided by the invention, in the whole operation process, only personnel are required to place samples in the sample bearing plate of the sample dividing plate, and then the counting and the collection of the samples are all automated, so that the manual operation of the personnel is reduced to the greatest extent, and the use convenience of the whole device is further improved.

Description

Microbial colony counter

Technical Field

The invention relates to the field of microorganism counting, in particular to a microorganism colony counting instrument.

Background

The determination of the total number of the bacterial colonies can judge the degree of the water quality polluted by bacteria, which reflects whether the water quality meets the quoted requirements of human beings or not, so as to make proper sanitation evaluation on the detected water quality; the total number of colonies indicates the quality of water to some extent. The colony sample cultured by the colony counter is counted, and the degree of water quality polluted by bacteria can be accurately judged.

Through retrieval, the Chinese patent discloses a microbial colony counter with the application number of 202111412040.3 and a counting method thereof, when the counter is used, a person manually puts a sample to be detected into a detection station, then performs amplification processing on the sample through an amplifier component, then photographs the amplified sample through a photographing camera, transmits the photographed photograph to an external control host for counting, and after the counting work is completed, the person manually takes off the sample on the detection station again and collects the sample uniformly. Although the automatic detection of batch is realized, the feeding and the blanking are operated by personnel, namely the current colony counting instrument is not convenient enough in actual use, the weight of manual operation by personnel is large, and some differences exist when the distance is completely realized automatically.

Disclosure of Invention

In order to solve the technical problems in the prior art, the invention provides the microbial colony counter, in the whole operation process, only personnel are required to place samples in the sample bearing disc of the sample dividing disc, then the counting and the collection of the samples are completely automated, the manual operation of the personnel is reduced to the maximum extent, and the use convenience of the whole device is further improved.

In order to achieve the purpose, the invention provides the following technical scheme: a microbial colony counter comprises a light shield fixed above a chassis through a support frame, wherein the light shield is of a cylindrical structure, a sample dividing disc and a first mounting plate are coaxially arranged in the light shield from bottom to top in sequence, and the sample dividing disc can move towards the first mounting plate along the axial direction of the light shield; a plurality of sample grooves are distributed on the sample dividing plate in an annular manner, a sample bearing plate capable of moving along the vertical direction is arranged in each sample groove, sliding plates with the number consistent with that of the sample grooves are distributed on the first mounting plate in an annular manner, and each sliding plate is in sliding connection with the first mounting plate along the radial direction of the first mounting plate; each sliding plate is internally provided with a sample through hole which is concentric with the sample groove and has the same specification, a first semicircular ring and a second semicircular ring are arranged in the sliding plate along the length direction of the sliding plate, the first semicircular ring and the second semicircular ring are mutually and slidingly guided in the sliding plate along the opposite/back movement direction, when the first semicircular ring and the second semicircular ring move oppositely, a circular ring structure which is concentric with the sample through hole is formed, and the inner diameter of the circular ring is smaller than that of the sample through hole; when the sample dividing disc moves towards the first mounting plate, the sample bearing disc in the sample groove firstly moves upwards to drive the sample to pass through the sample perforation, and then the first semicircular ring and the second semicircular ring move towards each other and are closed into a circular structure to form supporting and limiting on the sample; the slide plate is movable radially outwardly along the first mounting plate to extend through the light shield to an exterior thereof, and the first and second semi-circular rings are moved back toward each other to disengage the sample from the sample perforation as the sample perforation continues to move radially outwardly along the light shield after moving to the exterior of the light shield; the sample detached from the sample perforation slides along a spiral channel arranged on the outer wall of the light shield onto the chassis.

Preferably, a sliding groove for sliding the first semicircular ring and the second semicircular ring is formed in the sliding plate, the two sides of the second semicircular ring are integrally connected with a second linear rack along the sliding direction, the two sides of the first semicircular ring are connected with a first linear rack parallel to the second linear rack along the sliding direction, and the first linear rack and the second linear rack are in meshing transmission through a third gear; two be connected with the connection horizontal pole between the first straight-line rack, through connect the horizontal pole and move along its length direction in the sliding tray inside and order about first semicircle ring and second semicircle ring in opposite directions/back-to-back movement.

Preferably, the bottom of the sliding plate is provided with two guide sliding grooves communicated with the sliding grooves, the two connecting cross rods are connected through a first stop post, one ends of the two connecting cross rods opposite to each other are also connected with a sliding base, the sliding base extends to the upper part of the guide sliding grooves and is in sliding fit with the sliding plate, and a second stop post is connected between the extending ends of the sliding base; a limiting slide rail for limiting the sliding of the sliding plate is formed in the sliding plate corresponding to the periphery of the sliding seat; a second guide post is erected in the guide sliding chute along the length direction of the guide sliding chute, a sliding block is sleeved on the second guide post in a sliding mode, the top of the sliding block is connected with a first limiting piece matched with a first stop post through a connecting rod, the top of the first limiting piece is integrally connected with a second limiting piece matched with a second stop post, the sections of the first limiting piece and the second limiting piece along the length direction of the first limiting piece and the second limiting piece are both semicircular, the opening of the first limiting piece faces to one side where the first stop post is located, and the opening of the second limiting piece faces to one side where the second stop post is located; through the relative sliding of the sliding block and the sliding plate, the first limiting piece is matched with the first stopping column, and the second limiting piece is matched with the second stopping column, so that the connecting transverse rod is driven to move in the sliding groove along the length direction of the connecting transverse rod.

Preferably, the extending end of the sliding plate along the radial direction penetrates through the light shield and is connected with an arc baffle, the sliding plate is provided with limiting strips on two sides along the width direction, limiting strip sliding grooves are formed in the positions, corresponding to the limiting strips, in the first mounting plate, the sliding plate is assembled in the first mounting plate, and then a sliding guide matching structure is formed by matching the limiting strips with the limiting strip sliding grooves; the inner side end of the sliding plate is further connected with two sliding rods, the two sliding rods penetrate through the light shield along the length direction parallel to the sliding plate and then extend into the first mounting plate, a spring is connected between the extending end of each sliding rod and the tail end of the groove body in the first mounting plate, and the restoring force direction of the spring faces to the side where the arc-shaped baffle is located.

Preferably, a first guide post is fixedly connected to the center of the top of the chassis along the vertical direction, and the top of the first guide post extends upwards to be connected with the top of the inner side of the light shield; the sample dividing disc and the first mounting plate are respectively sleeved outside the first guide column; the sample dividing plate is characterized in that a first guide column below the first mounting plate is further sleeved with a third sleeve in a sliding mode, hinge rods corresponding to the number of the sliding plates are hinged to the circumferential surface of the third sleeve in an annular distribution mode, the tail end of each hinge rod is hinged to a hinge seat at the bottom of the corresponding sliding block, and the third sleeve is driven to slide upwards in the process that the sample dividing plate moves towards the first mounting plate along the first guide column, so that the hinge rods push the sliding blocks to slide inside the guide sliding grooves.

Preferably, two cylinders are symmetrically arranged on the top of the base plate, and piston ends of the two cylinders extend to the bottom of the sample dividing plate along the vertical direction and are connected with the sample dividing plate; the bottom of the sample groove forms a sealing structure through a fixed bottom plate, an electric telescopic rod is concentrically fixed at the top of the fixed bottom plate, and the movable end of the electric telescopic rod is connected with a sample bearing disc; two vacuumizing assemblies are further arranged at the bottom of the sample bearing disc, and air inlet holes of the vacuumizing assemblies extend upwards and penetrate through the sample bearing disc.

Preferably, each sliding plate is provided with a guide plate; the first guide post outside that is located first mounting panel top has cup jointed second sleeve pipe and second fixed ring board respectively from last to down, wherein the second sleeve pipe keeps sliding to cup joint with first guide post, second fixed ring board keeps fixed cup jointing with first guide post, second sleeve pipe bottom is fixed cup jointed the second ring-shaped rack that laminates with the second fixed ring board, second fixed ring board bottom fixed mounting has the second motor, the output drive of second motor is connected with the second gear with second ring-shaped rack looks meshing, it has the installation pole with deflector quantity looks adaptation to be annular distribution on the outer wall of second sleeve pipe, every the end of installation pole all rotates installs the roller, through the roller slides on corresponding deflector, makes the sliding plate slides along the radial of first mounting panel.

Preferably, the spiral passage includes from last second spiral passage and the first spiral passage who arranges in proper order down along the axial of lens hood, first spiral passage and second spiral passage parallel arrangement, and second spiral passage's length is less than first spiral passage, second spiral passage's discharge end corresponds first spiral passage's middle part, first spiral passage's discharge end extends to the chassis top.

Preferably, the microscope device further comprises a second mounting plate fixedly sleeved outside the first guide column, microscope assemblies matched with the sliding plates in number are distributed on the second mounting plate in an annular mode, each microscope assembly is concentrically arranged with the sample perforation on the sliding plate, a shooting camera capable of rotating around the axis of the first guide column is further arranged above the second mounting plate, and the shooting camera can be concentrically arranged with the corresponding microscope assembly at each rotation set angle.

Preferably, be located first fixed ring board and first sleeve pipe have been cup jointed from supreme down in proper order to the first guide post outside of second mounting panel top, wherein first fixed ring board keeps fixed cup jointing with first guide post, first sleeve pipe keeps rotating with first guide post and cup joints, first sleeve pipe bottom is connected with the first annular rack of laminating mutually with first fixed ring board, first motor is installed to the bottom of first fixed ring board, the output drive of first motor is connected with the first gear with first annular rack engaged with, follow its axis direction fixedly connected with first sleeve pipe of perpendicular to on the first sheathed tube outer wall, the shooting camera assembles in the terminal lower extreme of this first sleeve pipe.

Compared with the prior art, the invention provides a microbial colony counter which has the following beneficial effects:

(1) In the process that a sample moves towards the first mounting plate, acting force is applied to the third sleeve through the sample dividing plate, so that the first semicircular ring and the second semicircular ring in the sliding plate move oppositely to form a circular structure, the sample is supported and fixed in a sample through hole of the sliding plate, after the sample is fixed on the first mounting plate and counting processing is completed, the sliding plate is driven to move outwards along the radial direction of the first mounting plate through the rollers on the six mounting rods, after the sample on the sliding plate completely slides to the outside of the light shield, the second stop column is stopped by the second stop piece, and in the process that the sliding plate continuously slides outwards, the first semicircular ring and the second semicircular ring move towards directions away from each other, so that the sample is separated from the sample through hole and falls onto the spiral channel, and collection of the sample is completed finally. Whole operation process only needs personnel to place the sample in the sample carrier disc of sample graduated disk, and the count of sample and collection are whole to realize the automation afterwards, and the manual operation of personnel has been reduced to the at utmost, and then has promoted the convenience that whole equipment used.

(2) After the sample is placed on the sample bearing disc, the two vacuumizing assemblies are started to work to pump out air in the air inlet hole to form negative pressure, so that the sample is adsorbed and fixed on the sample bearing disc, and the sample is prevented from sliding off the sample bearing disc when passing through the sample perforation.

(3) Second spiral channel and the first spiral channel that the lens hood was arranged, the high-end of first spiral channel is close to adjacent three cowl, and the high-end of second spiral channel is then close to other three adjacent cowl, and two spiral channels can reduce the sample that releases from the sample perforation and drop to the height on the spiral channel, avoid leading to the sample to drop from the spiral channel because of highly too high. The length of second spiral passageway is less than first spiral passageway, and the discharge end of second spiral passageway corresponds first spiral passageway's middle part, and sample on the second spiral passageway is through discharge end and landing to first spiral passageway on, and first spiral passageway's discharge end extends to the chassis top, and all samples are finally piled up on the chassis through first spiral passageway is whole, and this kind of mode can avoid personnel's manual sample in perforating the sample to the sample one by one to dismantle.

(4) According to the invention, each sliding plate is uniformly provided with the guide plate, and when the mounting rod rotates anticlockwise, the roller pushes the guide plates to enable all six sliding plates to slide outwards along the radial direction of the first mounting plate, so that the sample collection efficiency is improved.

Drawings

The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the principles of the invention and not to limit the invention in any way:

fig. 1 is a schematic view of the overall structure of a counting device according to an embodiment;

FIG. 2 is a schematic diagram showing the distribution of two spiral passages in the embodiment;

FIG. 3 is a schematic overall view of another angle of the counting device according to the embodiment;

FIG. 4 is a schematic diagram showing an internal structure of the counting apparatus in the embodiment;

FIG. 5 is a schematic sectional view of a sample index plate according to an embodiment;

FIG. 6 is an enlarged view of a portion of the structure shown at A in FIG. 5;

FIG. 7 is a schematic view showing the assembly of the photographing camera on the first guide post in the embodiment;

FIG. 8 is a schematic structural view of the top of the first mounting plate in the embodiment;

FIG. 9 is a schematic structural view of the bottom of the first mounting plate in the embodiment;

FIG. 10 is a schematic view showing the assembly of the roller on the first guide post in the embodiment;

FIG. 11 is a schematic view showing the overall structure of the sliding plate according to the embodiment;

FIG. 12 is a schematic three-dimensional sectional view of the slide plate in the embodiment;

FIG. 13 is a schematic structural view at the first semicircular ring in the embodiment;

FIG. 14 is a schematic view showing various components of the slider in the embodiment;

FIG. 15 is a schematic view showing the structure of the bottom of the sliding plate in the embodiment;

fig. 16 is a schematic sectional view of the first mounting plate and the slide plate in the embodiment.

In the figure: 1. a chassis; 2. a support frame; 3. a light shield; 4. a first spiral channel; 5. a second spiral channel; 6. a first guide post; 7. a cylinder; 8. a sample index plate; 9. a first mounting plate; 10. a sliding plate; 11. a second mounting plate; 12. a microscope assembly; 13. a shooting camera; 14. fixing the bottom plate; 15. a sample carrying tray; 16. an electric telescopic rod; 17. a vacuum pumping assembly; 18. an air inlet; 19. a first stationary ring plate; 20. a first connecting rod; 21. a first sleeve; 22. a first annular rack; 23. a first motor; 24. a first gear; 25. a second sleeve; 26. mounting a rod; 27. a roller; 28. perforating the sample; 29. a guide plate; 30. a third sleeve; 31. a hinged lever; 32. a second annular rack; 33. a second stationary ring plate; 34. a second gear; 35. a second motor; 36. an arc-shaped baffle plate; 37. a limiting strip; 38. a first semicircular ring; 39. a second semi-circular ring; 40. a sliding groove; 41. a first linear rack; 42. a second linear rack; 43. a third gear; 44. connecting the cross bars; 45. a slide base; 46. a limiting slide rail; 47. a first stopper post; 48. a second stopper post; 49. a slider; 50. a first limit piece; 51. a connecting rod; 52. a second limiting member; 53. a through hole; 54. a hinged seat; 55. a slide bar; 56. a guide chute; 57. a second guide post; 58. a limiting strip chute; 59. a spring.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. The components of embodiments of the present invention generally described and illustrated in the figures herein may be arranged and designed in a wide variety of different configurations. Thus, the following detailed description of the embodiments of the present invention, presented in the figures, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments of the present invention without making any creative effort, shall fall within the protection scope of the present invention.

Referring to fig. 1-16, the present embodiment provides a microbial colony counter, which includes a light shield 3 fixed above a chassis 1 by a supporting frame 2, wherein the light shield 3 can prevent the device from being affected by an external light source during detection. Light shield 3 is cylindric structure, the center department at 1 top on chassis is along the first guide post 6 of vertical direction fixedly connected with, the top of first guide post 6 upwards extends to and links to each other with the top of light shield 3 inboard, supreme coaxial arrangement in proper order has sample graduated disk 8 and first mounting panel 9 from down on the first guide post 6, wherein sample graduated disk 8 is used for placing the sample that waits to detect, it can move towards first mounting panel 9 along the axial of light shield 3, so that with sample transfer in the sample graduated disk 8 to first mounting panel 9 in, and first mounting panel 9 is then fixed cup joints in first guide post 6 outsidely. Two air cylinders 7 are symmetrically arranged at the top of the base plate 1, piston ends of the two air cylinders 7 extend to the bottom of the sample dividing plate 8 in the vertical direction and are connected with the sample dividing plate 8, and the sample dividing plate 8 is driven to slide on the first guide column 6 through the two air cylinders 7.

In order to promote the detection efficiency of whole equipment, be the annular distribution in this application and be provided with a plurality of sample grooves on sample graduated disk 8, the bottom in every sample groove all forms seal structure through PMKD 14, and PMKD 14 top is fixed with electric telescopic handle 16 with one heart, and electric telescopic handle 16's expansion end bears dish 15 with the sample and links to each other, bears dish 15 through electric telescopic handle 16 drive sample and moves on vertical direction.

The first mounting plate 9 is annularly distributed with sliding plates 10 with the number consistent with that of the sample grooves, and each sliding plate 10 is in sliding connection with the first mounting plate 9 along the radial direction of the first mounting plate. Each slide plate 10 has a sample perforation 28 formed therein, which is concentric with and of the same size as the sample slot, and the interior of the slide plate 10 has a first semicircular ring 38 and a second semicircular ring 39 arranged along the length thereof, the first semicircular ring 38 and the second semicircular ring 39 are slidably guided inside the slide plate 10 in the facing/back movement direction with respect to each other, and when the first semicircular ring 38 and the second semicircular ring 39 move toward each other, a circular ring structure concentric with the sample perforation 28 is formed, and the inner diameter of the circular ring is smaller than the sample perforation 28 but larger than the cross-section of the sample-carrying tray 15.

It should be noted that the cross-section of the sample carrier plate 15 is smaller than the cross-section of the sample channels, so that the sample carrier plate 15 can be removed from the annular structure formed by the first semi-annular ring 38 and the second semi-annular ring 39. In order to improve the stability of the sample placed on the sample carrying tray 15, two vacuumizing assemblies 17 are further arranged at the bottom of the sample carrying tray 15, air inlet holes 18 of the vacuumizing assemblies 17 extend upwards and penetrate through the sample carrying tray 15, after the sample is placed on the sample carrying tray 15, the vacuumizing assemblies 17 are started to work to draw out air in the air inlet holes 18 to form negative pressure, so that the sample is fixed on the sample carrying tray 15 in an absorption mode, and the sample is prevented from sliding off the sample carrying tray 15 when passing through the sample through hole 28.

The counting device in this application still includes the fixed second mounting panel 11 that cup joints at first guide post 6 outside, it has the microscope unit 12 with sliding plate 10 quantity assorted to be the annular distribution on the second mounting panel 11, and every microscope unit 12 all keeps concentric setting with sample perforation 28 on the sliding plate 10, the top of second mounting panel 11 still is provided with can be around first guide post 6 axle center carry out the rotatory camera 13, the angle that every rotation of camera 13 set for can keep concentric with corresponding microscope unit 12, microscope unit 12 in this embodiment, sliding plate 10, the sample groove in the sample graduated disk 8 all sets up to six, consequently camera 13 sets up and can keep concentric with corresponding microscope unit 12 every rotatory 60, in addition, this application still is provided with a plurality of luminescent light sources at the top of light shield 3 inboard, a plurality of luminescent light sources all are connected with the outside through cable with shooting camera 13.

In the embodiment, the sample to be tested is firstly placed in the six sample grooves on the sample dividing plate 8, since the outer diameter of the sample is consistent with the inner diameter of the sample groove, the sample is embedded in the sample groove under the restriction of the sample groove and the support of the sample carrying plate 15, then the vacuum pumping assembly 17 is started to drive the sample dividing plate 8 to slide upwards on the first guide column 6, when the sample dividing plate 8 approaches the first mounting plate 9, the electric telescopic rod 16 in the sample groove is operated to drive the sample carrying plate 15 to move upwards and make the sample pass through the sample perforation 28, then the first semicircular ring 38 and the second semicircular ring 39 move towards each other to close into a circular ring structure, since the inner diameter of the circular ring structure is larger than the cross section of the sample carrying plate 15, therefore, even when the sample dividing plate 8 moves downwards during the resetting process of the electric telescopic rod 16, the circular ring formed by the first semicircular ring 38 and the second semicircular ring 39 does not affect the movement, and the sample carried on the sample carrying plate 15 can be blocked by the circular ring, and finally the sample is located in the sample perforation 28 and is supported and fixed by the circular ring. When the sample is fixed in the sample perforation 28, the six microscope assemblies 12 on the second mounting plate 11 can magnify the corresponding sample, and then the photographing camera 13 sequentially performs photographing counting processing on the magnified sample by rotation. When all the samples have been subjected to the counting test, the slide plate 10 is moved radially outward along the first mounting plate 9 to extend through the light shield 3 to the outside thereof, and when the sample perforation 28 continues to move radially outward along the light shield 3 after moving to the outside of the light shield 3, the first semicircular ring 38 and the second semicircular ring 39 are moved back toward each other to detach the sample from the sample perforation 28, and the sample detached from the sample perforation 28 is slid onto the chassis 1 along the spiral passage arranged on the outer wall of the light shield 3.

In addition to the above solution, as shown in fig. 12, a sliding groove 40 for sliding the first semicircular ring 38 and the second semicircular ring 39 is formed in the sliding plate 10, a second linear rack 42 is integrally connected to both sides of the second semicircular ring 39 along the sliding direction thereof, a first linear rack 41 parallel to the second linear rack 42 is connected to both sides of the first semicircular ring 38 along the sliding direction thereof, and the first linear rack 41 and the second linear rack 42 are in meshing transmission by arranging a third gear 43. A connecting cross bar 44 is connected between the two first linear gears 41, and the first semi-circular ring 38 and the second semi-circular ring 39 are driven to move towards/away from each other by moving the connecting cross bar 44 in the sliding groove 40 along the length direction thereof, wherein when the connecting cross bar 44 slides in the sliding groove 40 towards the arc baffle 36, the first semi-circular ring 38 and the second semi-circular ring 39 move towards each other, and otherwise, the first semi-circular ring 38 and the second semi-circular ring 39 move away from each other.

In order to realize the sliding of the driving connecting cross bar 44 in the sliding groove 40, as shown in fig. 12 to 15, in this embodiment, a guide sliding groove 56 communicated with the sliding groove 40 is formed at the bottom of the sliding plate 10, the number of the connecting cross bars 44 is two, the two connecting cross bars 44 are connected through a first stop column 47, a sliding seat 45 is further connected to one end of each of the two connecting cross bars 44, the sliding seat 45 extends to the upper side of the guide sliding groove 56 and keeps sliding fit with the sliding plate 10, and a second stop column 48 is connected between the extending ends of the sliding seat 45. The inner part of the sliding plate 10 is formed with a limiting slide rail 46 corresponding to the periphery of the sliding seat 45 for limiting the sliding thereof, the sliding seat 45 can drive the connecting cross bar 44 to slide in the sliding groove 40 when sliding along the length direction of the sliding plate 10, and the limiting slide rail 46 at the periphery of the sliding seat 45 can limit the movement of the sliding seat 45 to ensure that the sliding seat 45 always moves along a straight line. Further, in the present embodiment, a second guiding post 57 is erected in the guiding sliding groove 56 along the length direction thereof, the sliding block 49 is slidably sleeved on the second guiding post 57, a through hole 53 adapted to the second guiding post 57 is formed in the sliding block 49 in a penetrating manner, the top of the sliding block 49 is connected with a first limiting member 50 adapted to the first stopping post 47 through a connecting rod 51, the top of the first limiting member 50 is integrally connected with a second limiting member 52 adapted to the second stopping post 48, the cross sections of the first limiting member 50 and the second limiting member 52 along the length direction thereof are both semicircular, the opening of the first limiting member 50 faces to the side where the first stopping post 47 is located, and the opening of the second limiting member 52 faces to the side where the second stopping post 48 is located. Through the relative sliding of the sliding block 49 and the sliding plate 10, the first limiting member 50 is engaged with the first stopping post 47, and the second limiting member 52 is engaged with the second stopping post 48, so as to drive the connecting cross bar 44 to move along the length direction inside the sliding groove 40, specifically, when the sliding block 49 moves inside the guide sliding groove 56 to approach the first stopping post 47 in the direction of approaching the first semicircular ring 38, the first limiting member 50 above the sliding block is blocked by the first stopping post 47, and further, the connecting cross bar 44 is driven to slide inside the sliding groove 40. After the sample is detected, the sliding plate 10 moves outward along the radial direction of the first mounting plate 9, the sliding block 49 is firstly kept immovable, after the sample through hole 28 completely moves to the outside of the light shield 3, the connecting rod 51 is just stopped by the second stopping post 48, when the sliding plate 10 continues to slide outward, the immovable first limiting member 50 applies an acting force to the second stopping post 48, so that the first semicircular ring 38 and the second semicircular ring 39 slide back and forth in the sliding groove 40 relative to the sliding plate 10, the sample is further separated from the circular ring, and the separated sample just falls to the spiral channel on the outer wall of the light shield 3 and slides to the chassis 1 through the spiral channel.

The radial extending end of the sliding plate 10 penetrates through the light shield 3 and then is connected with an arc-shaped baffle plate 36, when the sliding plate 10 is at an initial position, the arc-shaped baffle plate 36 is located outside the light shield 3 and can limit the movement of the sliding plate 10 towards the direction of the circle center of the first mounting plate 9, limiting strips 37 are formed on two sides of the sliding plate 10 along the width direction of the sliding plate, limiting strip sliding grooves 58 are formed in positions, corresponding to the limiting strips 37, in the first mounting plate 9, the sliding plate 10 is assembled inside the first mounting plate 9, and then a sliding guide matching structure is formed by matching the limiting strips 37 with the limiting strip sliding grooves 58. The inner end of the sliding plate 10 is further connected with two sliding rods 55, the two sliding rods 55 penetrate through the light shield 3 along the length direction parallel to the sliding plate 10 and extend into the first mounting plate 9, a spring 59 is connected between the extending end of the sliding rod 55 and the tail end of the groove body inside the first mounting plate 9, the restoring force direction of the spring 59 faces to the side away from the arc-shaped baffle plate 36, after a sample is detected, the sliding plate 10 slides towards the outside of the light shield 3, the sliding rod 55 slides out of the first mounting plate 9 and stretches the spring 59 to deform, after the sample perforation 28 completely slides to the outside of the light shield 3, the sample breaks away from the sample perforation 28 and falls to the spiral channel, and the whole sliding plate 10 returns to the initial position under the restoring force action of the sliding rod 55.

In order to drive the slide block 49 to move inside the guide chute 56 during the upward movement of the sample dividing disk 8, in this embodiment, a third sleeve 30 is slidably sleeved on the first guide column 6 below the first mounting plate 9, six hinge rods 31 are annularly distributed and hinged on the circumferential surface of the third sleeve 30, the end of each hinge rod 31 is hinged on a hinge seat 54 at the bottom of the corresponding slide block 49, and when the vacuum pumping assembly 17 drives the sample dividing disk 8 to move along the first guide column 6 to the first mounting plate 9, the sample dividing disk 8 exerts a force on the third sleeve 30 to drive it to slide upward, so that the hinge rods 31 push the slide block 49 to slide inside the guide chute 56 in a direction close to the first semicircular ring 38.

In order to improve the working efficiency, the six sliding plates 10 move outwards synchronously, and in this embodiment, each sliding plate 10 is provided with a guide plate 29, and the arrangement form of the guide plates 29 is shown in the second mounting plate 11. A second sleeve 25 and a second fixed ring plate 33 are respectively sleeved outside the first guide column 6 above the first mounting plate 9 from top to bottom, wherein the second sleeve 25 is slidably sleeved with the first guide column 6, the second fixed ring plate 33 is fixedly sleeved with the first guide column 6, a second annular rack 32 attached to the second fixed ring plate 33 is fixedly sleeved at the bottom of the second sleeve 25, a second motor 35 is fixedly mounted at the bottom of the second fixed ring plate 33, a second gear 34 engaged with the second annular rack 32 is connected to the output end of the second motor 35 in a driving manner, six mounting rods 26 are annularly distributed on the outer wall of the second sleeve 25, a roller 27 is rotatably mounted at the tail end of each mounting rod 26, the second gear 34 is driven to rotate by the second motor 35, the second gear 34 is engaged with the second linear rack 42, so that the second sleeve 25 is driven to rotate outside the first guide column 6, the six mounting rods 26 rotate synchronously, and in an initial state, the roller 27 at the tail end of each mounting rod 26 and the guide plate 29 are radially pushed by the sliding plate 9 in a counterclockwise direction as shown in fig. 10.

As a preferred embodiment, the spiral channel in this embodiment is as shown in fig. 1 to fig. 3, and includes a second spiral channel 5 and a first spiral channel 4 which are sequentially arranged from top to bottom along the axial direction of the light shield 3, the first spiral channel 4 and the second spiral channel 5 are arranged in parallel, the high end of the first spiral channel 4 is close to three adjacent arc-shaped baffles 36, and the high end of the second spiral channel 5 is close to the other three adjacent arc-shaped baffles 36, and the arrangement of the two spiral channels can reduce the height of the sample released from the sample perforation 28 falling onto the spiral channel, and avoid the sample falling off the spiral channel due to the too high height. In addition, the length of second spiral passage 5 is less than first spiral passage 4, and the discharge end of second spiral passage 5 corresponds the middle part of first spiral passage 4, and the sample on second spiral passage 5 passes through the discharge end and on landing to first spiral passage 4, and the discharge end of first spiral passage 4 extends to chassis 1 top, and all samples are finally piled up on chassis 1 through first spiral passage 4 is whole, and this kind of mode can avoid personnel to dismantle the sample in sample perforation 28 manually one by one.

As a preferred embodiment, a first fixed ring plate 19 and a first sleeve 21 are sequentially sleeved outside a first guide post 6 located above a second mounting plate 11 from bottom to top, wherein the first fixed ring plate 19 and the first guide post 6 are fixedly sleeved, the first sleeve 21 and the first guide post 6 are rotatably sleeved, the bottom of the first sleeve 21 is connected with a first annular rack 22 attached to the first fixed ring plate 19, the bottom of the first fixed ring plate 19 is provided with a first motor 23, the output end of the first motor 23 is in driving connection with a first gear 24 engaged with the first annular rack 22, the outer wall of the first sleeve 21 is fixedly connected with the first sleeve 21 along a direction perpendicular to the axis thereof, the photographing camera 13 is assembled at the lower end of the first sleeve 21, in order to save the manufacturing cost of the entire apparatus, only one photographing camera 13 is provided in this embodiment, the photographing camera 13 rotates around the first guide post 6 to complete the counting operation of six microscopic photographing lenses 12, and the rotation of the photographing camera 13 is the same as the rotation operation principle of the roller 27, which is not repeated.

The invention provides a microbial colony counter, which has the specific working principle that: the sample to be detected is placed on the sample bearing disc 15 of the sample groove, then the vacuumizing assembly 17 is started to drive the sample dividing disc 8 to move upwards to be close to the first mounting plate 9, and after the sample dividing disc 8 moves upwards to enter the light shield 3, the interior of the light shield 3 can be completely sealed to form a sealed environment, so that the influence of an external light source on the detection of the light shield is avoided. When the sample dividing disc 8 approaches the first mounting plate 9, the electric telescopic rod 16 is started to drive the sample carrying disc 15 to move upwards and ensure that a sample smoothly passes through the sample perforation 28, the sample dividing disc 8 continues to move upwards until contacting with the third sleeve 30, the sample dividing disc 8 pushes the third sleeve 30 to move upwards, the slide block 49 is driven by the hinge rod 31 to slide towards the first semicircular ring 38 in the guide chute 56, the first limiting part 50 above the slide block 49 is blocked by the first stopping column 47 and further pushes the connecting cross rod 44 to slide in the slide groove 40, the first semicircular ring 38 and the second semicircular ring 39 move towards each other in the slide groove 40 to form a circular ring structure, at the moment, the vacuum pumping assembly 17 stops moving, the third sleeve 30 is fixed on the first guide column 6 and is kept still, at the moment, the telescopic end of the electric telescopic rod 16 retracts, the whole sample is fixed in the perforation 28 and supported by the circular ring structure formed by the first semicircular ring 38 and the second semicircular ring 39, the six microscope assemblies 12 on the second mounting plate 11 shoot the whole sample, and the microscope assembly 12 and transmit the amplified data to the microscope assembly 13, and control the microscope assembly to complete the amplification processing.

After all the samples are counted, the second motor 35 is started to drive the mounting rod 26 to rotate, each roller 27 acts on the corresponding guide plate 29 on the sliding plate 10, the sliding plate 10 is pushed to move radially outward along the first mounting plate 9, at this time, the sliding block 49 is kept stationary inside the guide sliding chute 56, the first semicircular ring 38 and the second semicircular ring 39 are also fixed to the samples in an annular structure, after the sample perforation 28 on the sliding plate 10 completely moves to the outside of the light shield 3, the connecting rod 51 is just blocked by the second blocking column 48, when the sliding plate 10 continues to move to the outside of the light shield 3, the connecting rod 51 applies acting force to the second blocking column 48 to move the first semicircular ring 38 and the second semicircular ring 39 in directions away from each other, at this time, the samples lose the supporting force of the first semicircular ring 38 and the second semicircular ring 39 and fall from the sample perforation 28 onto the spiral channel, and the six samples slide onto the chassis 1 under the guiding action of the first spiral channel 4 and the second spiral channel 5.

In the description of the present invention, the terms "first", "second", "another", and "yet" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implying any number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more features. In the description of the embodiments of the present invention, "a plurality" means two or more unless specifically defined otherwise.

In the description of the present invention, it is to be noted that, unless otherwise explicitly specified or limited, the terms "connected" and "connected" are to be interpreted broadly, e.g., as being fixed or detachable or integrally connected; can be mechanically or electrically connected; may be directly connected or indirectly connected through an intermediate. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art. In addition, in the description of the present invention, "a plurality" means two or more unless otherwise specified.

While embodiments of the invention have been shown and described, it will be understood by those of ordinary skill in the art that: many changes, modifications, substitutions and alterations can be made to these embodiments without departing from the spirit and scope of the invention, which is defined by the claims and their equivalents.

Claims

1. The utility model provides a microorganism bacterial colony count appearance, includes fixes lens hood (3) in chassis (1) top through support frame (2), its characterized in that: the light shield (3) is of a cylindrical structure, a sample dividing disc (8) and a first mounting plate (9) are coaxially arranged in the light shield from bottom to top in sequence, and the sample dividing disc (8) can move towards the first mounting plate (9) along the axial direction of the light shield (3); a plurality of sample grooves are annularly distributed on the sample dividing plate (8), a sample bearing plate (15) capable of moving along the vertical direction is arranged in each sample groove, sliding plates (10) with the number consistent with that of the sample grooves are annularly distributed on the first mounting plate (9), and each sliding plate (10) is in sliding connection with the first mounting plate (9) along the radial direction of the first mounting plate; each sliding plate (10) is internally provided with a sample perforation (28) which is concentric with and has the same specification as the sample groove, the inner part of the sliding plate (10) is provided with a first semicircular ring (38) and a second semicircular ring (39) along the length direction of the sliding plate, the first semicircular ring (38) and the second semicircular ring (39) are mutually and slidably guided in the inner part of the sliding plate (10) along the opposite/back movement direction, when the first semicircular ring (38) and the second semicircular ring (39) move in opposite directions, a circular ring structure which is concentric with the sample perforation (28) is formed, and the inner diameter of the circular ring is smaller than that of the sample perforation (28); during the movement of the sample indexing disc (8) towards the first mounting plate (9), the sample carrying disc (15) in the sample groove firstly moves upwards to drive the sample to pass through the sample perforation (28), and then the first semicircular ring (38) and the second semicircular ring (39) move towards each other to be closed into a circular ring structure so as to form a supporting limit for the sample; the sliding plate (10) can move radially outwards along the first mounting plate (9) and extends to the outside of the light shield (3) through the light shield (3), and when the sample perforation (28) continues to move radially outwards along the light shield (3) after moving to the outside of the light shield (3), the first semicircular ring (38) and the second semicircular ring (39) move back towards each other to separate the sample from the sample perforation (28); the sample detached from the sample perforation (28) slides onto the chassis (1) along a spiral channel arranged on the outer wall of the light shield (3);

the center of the top of the chassis (1) is fixedly connected with a first guide post (6) along the vertical direction, and the top of the first guide post (6) extends upwards to be connected with the top of the inner side of the light shield (3); the sample dividing disc (8) and the first mounting plate (9) are respectively sleeved outside the first guide column (6); a third sleeve (30) is further sleeved on the first guide column (6) below the first mounting plate (9) in a sliding manner, hinged rods (31) corresponding to the number of the sliding plates (10) are hinged on the circumferential surface of the third sleeve (30) in an annular distribution manner, the tail end of each hinged rod (31) is hinged to a hinged seat (54) at the bottom of a corresponding sliding block (49), and the third sleeve (30) is driven to slide upwards in the process that the sample dividing disc (8) moves towards the first mounting plate (9) along the first guide column (6), so that the hinged rods (31) push the sliding blocks (49) to slide in the guide sliding grooves (56);

the top of the chassis (1) is also symmetrically provided with two cylinders (7), and the piston ends of the two cylinders (7) extend to the bottom of the sample dividing plate (8) along the vertical direction and are connected with the sample dividing plate; the bottom of the sample groove forms a sealing structure through a fixed bottom plate (14), an electric telescopic rod (16) is concentrically fixed at the top of the fixed bottom plate (14), and the movable end of the electric telescopic rod (16) is connected with a sample bearing disc (15); two vacuumizing assemblies (17) are further arranged at the bottom of the sample bearing disc (15), and air inlet holes (18) of the vacuumizing assemblies (17) extend upwards and penetrate through the sample bearing disc (15);

the microscope assembly structure is characterized by further comprising a second mounting plate (11) fixedly sleeved outside the first guide column (6), microscope assemblies (12) matched with the sliding plates (10) in number are distributed on the second mounting plate (11) in an annular mode, each microscope assembly (12) is concentrically arranged with a sample through hole (28) in each sliding plate (10), a shooting camera (13) capable of rotating around the axis of the first guide column (6) is further arranged above the second mounting plate (11), and the angle set by each rotation of the shooting camera (13) can be concentric with the corresponding microscope assembly (12).

2. A microbial colony counter according to claim 1, wherein: a sliding groove (40) for sliding a first semicircular ring (38) and a second semicircular ring (39) is formed in the sliding plate (10), a second linear rack (42) is integrally connected to two sides of the second semicircular ring (39) along the sliding direction of the second semicircular ring, a first linear rack (41) parallel to the second linear rack (42) is connected to two sides of the first semicircular ring (38) along the sliding direction of the first semicircular ring, and the first linear rack (41) and the second linear rack (42) are in meshing transmission by arranging a third gear (43); a connecting cross rod (44) is connected between the two first linear racks (41), and the first semicircular ring (38) and the second semicircular ring (39) are driven to move towards/away from each other by the connecting cross rod (44) moving along the length direction of the sliding groove (40) inside the sliding groove.

3. The microbial colony counter of claim 2, wherein: the bottom of the sliding plate (10) is provided with guide sliding grooves (56) communicated with the sliding groove (40), the number of the connecting cross rods (44) is two, the two connecting cross rods (44) are connected through first stop posts (47), one ends, opposite to the two connecting cross rods (44), are also connected with sliding seats (45), the sliding seats (45) extend to the upper portion of the guide sliding grooves (56) and are in sliding fit with the sliding plate (10), and second stop posts (48) are connected between the extending ends of the sliding seats (45); a limiting slide rail (46) for limiting the sliding of the sliding plate (10) is formed in the sliding plate (10) and corresponds to the periphery of the sliding seat (45); a second guide post (57) is erected in the guide sliding groove (56) along the length direction of the guide sliding groove, a sliding block (49) is sleeved on the second guide post (57) in a sliding mode, the top of the sliding block (49) is connected with a first limiting piece (50) matched with the first stop post (47) through a connecting rod (51), the top of the first limiting piece (50) is integrally connected with a second limiting piece (52) matched with the second stop post (48), the sections of the first limiting piece (50) and the second limiting piece (52) along the length direction of the first limiting piece and the second limiting piece are both semicircular, the opening of the first limiting piece (50) faces to one side where the first stop post (47) is located, and the opening of the second limiting piece (52) faces to one side where the second stop post (48) is located; through the relative sliding of the sliding block (49) and the sliding plate (10), the first limiting piece (50) is matched with the first stop column (47), and the second limiting piece (52) is matched with the second stop column (48), so that the connecting transverse rod (44) is driven to move in the length direction of the sliding groove (40).

4. A microbial colony counter according to claim 3, wherein: the extending end of the sliding plate (10) in the radial direction penetrates through the light shield (3) and then is connected with an arc-shaped baffle (36), limiting strips (37) are formed on the two sides of the sliding plate (10) in the width direction, limiting strip sliding grooves (58) are formed in the positions, corresponding to the limiting strips (37), in the first mounting plate (9), and after the sliding plate (10) is assembled in the first mounting plate (9), the limiting strips (37) and the limiting strip sliding grooves (58) are matched to form a sliding guide matching structure; the inner side end of the sliding plate (10) is further connected with two sliding rods (55), the two sliding rods (55) penetrate through the light shield (3) along the length direction parallel to the sliding plate (10) and then extend into the first mounting plate (9), a spring (59) is connected between the extending end of each sliding rod (55) and the tail end of the groove body in the first mounting plate (9), and the restoring force direction of the spring (59) faces to the side away from the arc-shaped baffle (36).

5. A microbial colony counter according to claim 4, wherein: a guide plate (29) is arranged on each sliding plate (10); the outer portion of the first guide column (6) above the first mounting plate (9) is sleeved with a second sleeve (25) and a second fixing ring plate (33) from top to bottom, the second sleeve (25) and the first guide column (6) are in sliding sleeve connection, the second fixing ring plate (33) and the first guide column (6) are in fixed sleeve connection, a second annular rack (32) attached to the second fixing ring plate (33) is fixedly sleeved at the bottom of the second sleeve (25), a second motor (35) is fixedly mounted at the bottom of the second fixing ring plate (33), the output end of the second motor (35) is in driving connection with a second gear (34) meshed with the second annular rack (32), mounting rods (26) matched with the number of the guide plates (29) are distributed on the outer wall of the second sleeve (25) in an annular mode, the tail end of each mounting rod (26) is rotatably mounted with a roller (27), and the sliding plate (29) slides on the corresponding guide plate (29) along the radial direction of the first sliding plate (9).

6. A microbial colony counter according to claim 1, wherein: spiral channel includes from last second spiral channel (5) and the first spiral channel (4) that arrange down in proper order along the axial of lens hood (3), first spiral channel (4) and second spiral channel (5) parallel arrangement, and the length of second spiral channel (5) is less than first spiral channel (4), the discharge end of second spiral channel (5) corresponds the middle part of first spiral channel (4), the discharge end of first spiral channel (4) extends to chassis (1) top.

7. A microbial colony counter according to claim 1, wherein: be located first guide post (6) outside of second mounting panel (11) top is supreme down has cup jointed first fixed ring board (19) and first sleeve pipe (21) in proper order, wherein first fixed ring board (19) keep fixed cup jointing with first guide post (6), first sleeve pipe (21) keep rotating with first guide post (6) and cup joint, first sleeve pipe (21) bottom is connected with first annular rack (22) of laminating mutually with first fixed ring board (19), first motor (23) are installed to the bottom of first fixed ring board (19), the output drive of first motor (23) is connected with first gear (24) with first annular rack (22) engaged with, along its axis direction fixedly connected with first sleeve pipe (21) of perpendicular to on the outer wall of first sleeve pipe (21), camera (13) assemble in this terminal lower extreme of first sleeve pipe (21).