CN117286019A - Automatic enrichment and storage device for environmental DNA samples - Google Patents
Automatic enrichment and storage device for environmental DNA samples Download PDFInfo
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- CN117286019A CN117286019A CN202210697027.5A CN202210697027A CN117286019A CN 117286019 A CN117286019 A CN 117286019A CN 202210697027 A CN202210697027 A CN 202210697027A CN 117286019 A CN117286019 A CN 117286019A
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- 238000003860 storage Methods 0.000 title claims abstract description 207
- 230000007613 environmental effect Effects 0.000 title claims abstract description 69
- 239000012528 membrane Substances 0.000 claims abstract description 199
- 238000012545 processing Methods 0.000 claims abstract description 132
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 47
- 238000001914 filtration Methods 0.000 claims abstract description 41
- 230000007246 mechanism Effects 0.000 claims description 33
- 230000000712 assembly Effects 0.000 claims description 14
- 238000000429 assembly Methods 0.000 claims description 14
- 238000001035 drying Methods 0.000 claims description 11
- 230000033001 locomotion Effects 0.000 claims description 8
- 238000012546 transfer Methods 0.000 claims description 7
- 210000000078 claw Anatomy 0.000 claims description 6
- 230000006698 induction Effects 0.000 claims description 5
- 230000001939 inductive effect Effects 0.000 claims description 3
- 238000004321 preservation Methods 0.000 abstract description 20
- 238000005070 sampling Methods 0.000 abstract description 13
- 238000000034 method Methods 0.000 description 6
- 238000003825 pressing Methods 0.000 description 6
- 230000000694 effects Effects 0.000 description 5
- 238000000967 suction filtration Methods 0.000 description 5
- 238000010586 diagram Methods 0.000 description 4
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- 239000000203 mixture Substances 0.000 description 3
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- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 230000005540 biological transmission Effects 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 238000009826 distribution Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000011156 evaluation Methods 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 230000007774 longterm Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000005086 pumping Methods 0.000 description 2
- 238000007789 sealing Methods 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 239000002274 desiccant Substances 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 230000003028 elevating effect Effects 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 238000010030 laminating Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 230000013011 mating Effects 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 238000011112 process operation Methods 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000000741 silica gel Substances 0.000 description 1
- 229910002027 silica gel Inorganic materials 0.000 description 1
- 239000002352 surface water Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 230000001360 synchronised effect Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
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- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
- G01N35/04—Details of the conveyor system
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Abstract
The invention discloses an automatic enrichment and storage device for an environment DNA sample, which comprises the steps of firstly, moving a sample processing component into a sample storage unit through a conveying unit, then, moving a filter membrane fixing component onto a sample filtering unit through the conveying unit, then, moving the filter membrane fixing component which completes sample enrichment onto the sample processing component in the sample storage unit through the conveying unit, and finally, moving the sample processing component onto the filter membrane fixing component which completes sample enrichment through the conveying unit; according to the scheme, the devices are matched in a cooperative manner, so that the automatic sampling and preservation of a plurality of environmental DNA samples are realized, the problem of sample pollution possibly caused by manual operation is solved, the enrichment and storage efficiency of the environmental DNA samples is improved, the continuous automatic enrichment of the environmental DNA samples in a plurality of water samples and the continuous automatic preservation requirement of the environmental DNA samples in a filter membrane are met, and the automatic sampling and preservation device is high in practicability and suitable for wide popularization and application.
Description
Technical Field
The invention relates to the technical field of water quality sampling, in particular to an automatic enrichment and storage device for an environment DNA sample.
Background
Biodiversity is an important foundation for human survival and development, and the composition of aquatic biocenosis is a main research object of species diversity of an aquatic ecosystem, and plays a vital role in implementing biodiversity protection measures and evaluating aquatic ecological health. The acquisition of DNA from the water environment to reflect the information of aquatic organism species composition, distribution and abundance (environmental DNA bar code technology) is a non-invasive and rapid detection means, and has been gradually applied to the evaluation of water ecological organism diversity and water ecological health.
Ambient DNA enrichment is an important ring in the application of ambient DNA barcode technology, directly affecting aquatic organism species composition. Reliability of distribution and abundance information. At present, a wide-mouth bottle with larger capacity is generally adopted for collecting surface water samples, or a water sampler is used for collecting water samples of water layers with different depths, and then the water samples are filtered and enriched with DNA in the water. The whole process mainly uses manpower, the quantity of sample collection depends on the quantity of the carried sample bottles to a great extent, the whole transportation is inconvenient, particularly, the water body with high suspended matters is treated, the filtering difficulty is high, the filtering time is long, the working efficiency is seriously influenced, in addition, in the preservation mode, the environmental DNA filter membrane obtained by laboratory suction filtration is stored in a low-temperature frozen manner, the environmental DNA filter membrane obtained by field suction filtration is stored in an alcohol or silica gel dry manner, and the filter membrane preservation aging and the efficiency are related to the preservation method. When a plurality of water samples are required to be filtered, the filter membrane and the clamping filter flask are required to be repeatedly replaced, the filtered filter membrane is taken down and placed in alcohol or a sealed bag containing a drying agent or a refrigerator for freezing and preserving, the process operation is complex, the working efficiency is low, and long-time and automatic collection of a plurality of samples cannot be met.
In order to satisfy the demand in novel monitoring market, improve water environment DNA sampling enrichment work efficiency and reduce open-air sampling risk, the patent of application number CN202020932192.0 discloses a collection device of estuary water environment DNA sample, and it includes support body pole, support body board and suction pump body, support body board lower surface welding has the support body pole, the inside collection box that has pegged graft of support body board, collection box internally mounted has the filter, collection box installs the drainage bucket in the lower extreme, the liquid sampling bottle is installed to the drainage bucket lower extreme, the suction pump body is installed to support body board upper surface one side, the ripple nest of tubes is installed to suction pump body output, the pipe installation piece is installed to the ripple nest of tubes end, the inlet tube is installed to suction pump body input, the suction bellows is installed to the inlet tube end, the suction bellows is installed to the suction bellows end. According to the technical scheme, although automatic sampling and enrichment of water environment DNA are realized to a certain extent, continuous automatic enrichment and preservation of the environment DNA cannot be realized.
Therefore, based on the defects of the prior art, a high-efficiency automatic enrichment and storage device for water environment DNA is developed, the authenticity and the effectiveness of the collected environmental DNA sample are ensured, continuous and automatic collection and storage are realized, the artificial interference factors are reduced, and the device has important roles in water ecological organism diversity and water ecological health evaluation.
Disclosure of Invention
The invention provides an automatic enrichment and storage device for an environment DNA sample, which aims to solve the technical problems that manual participation is needed, working efficiency is low, filter membrane replacement operation is complicated and filter membrane storage is inconvenient in the existing eDNA sampling and storage process.
According to one aspect of the present invention, there is provided an automatic enrichment and storage device for an environmental DNA sample, comprising: a filter membrane fixing assembly storage unit storing a plurality of filter membrane fixing assemblies for filtering water samples through the filter membrane to extract samples; the sample filtering unit is used for carrying out sample enrichment by being matched with the filter membrane fixing component; a sample processing module storage unit storing a plurality of sample processing modules for processing a sample to preserve the sample; a sample storage unit for storing the filter membrane fixing component and/or the sample processing component; and a transfer unit for moving the filter membrane holder assembly between the filter membrane holder assembly storage unit, the sample filtration unit and the sample storage unit, and/or for moving the sample processing assembly between the sample processing assembly storage unit and the sample storage unit.
As a further improvement of the above technical scheme:
further, the conveying unit comprises a mounting frame, a movable shaft movably arranged on the mounting frame, a rotation driving piece which is arranged on the mounting frame and connected with the movable shaft and used for driving the movable shaft to circumferentially rotate, a movement driving piece which is arranged on the mounting frame and connected with the movable shaft and used for driving the movable shaft to vertically move, and a clamping arm which is fixed on the movable shaft and used for clamping the filter membrane fixing assembly.
Further, the conveying unit further comprises a positioning mechanism for determining the rotation position of the clamping end of the conveying unit so as to ensure that the clamping arm accurately clamps the filter membrane fixing component, the positioning mechanism comprises a dial sleeved on the movable shaft, a plurality of scale grooves which are arranged in one-to-one correspondence with the filter membrane fixing component storage unit, the sample filtering unit and the sample storage unit are formed in the dial, and the positioning mechanism further comprises an induction controller which is electrically connected with the rotation driving piece and is used for inducing the scale grooves to control the rotation driving piece to work so as to control the rotation of the clamping arm.
Further, the positioning mechanism further comprises a plurality of positioning holes which are arranged on the mounting frame and are in one-to-one correspondence with the filter membrane fixing assembly storage unit, the sample filtering unit and the sample storage unit, and positioning bosses which are arranged on the clamping arms and are used for being in plug-in fit with the positioning holes to position the clamping arms.
Further, the sample processing assembly includes a disc-shaped body for stacking connection with the filter membrane securing assembly and a drying sheet disposed on the disc-shaped body for drying the filter membrane to preserve the sample.
Further, the sample processing component storage unit comprises a processing component storage cylinder body for storing the sample processing components and a first lifting mechanism which is arranged in the processing component storage cylinder body and used for driving the sample processing components to axially lift, and a plurality of sample processing components are axially stacked in the sample processing component storage unit.
Further, the sample processing component storage unit further comprises movable claws which are movably arranged along the radial direction of the sample processing component storage cylinder and used for being clamped between two adjacent sample processing components on the sample processing component storage cylinder so as to facilitate separation of the two adjacent sample processing components.
Further, the sample filtering unit comprises a suction filter bottle, an inner sleeve extending into the bottle is arranged at the bottle opening of the suction filter bottle, a suction filter tip for connecting a vacuum pump is arranged on the bottle body of the suction filter bottle, and the axial height of the suction filter tip is higher than that of the lower end of the inner sleeve.
Further, the filter membrane fixing component storage unit comprises a filter membrane fixing component storage cylinder body for storing the filter membrane fixing components and a second lifting mechanism which is arranged in the filter membrane fixing component storage cylinder body and used for driving the filter membrane fixing components to axially lift, and the filter membrane fixing components are axially stacked in the filter membrane fixing component storage unit.
Further, the filter membrane fixing assembly further comprises an outer clamping ring and an inner clamping ring, wherein the outer clamping ring is used for supporting the lower end face of the filter membrane, and the inner clamping ring is attached to the inner wall face of the outer clamping ring and abuts against the upper end face of the filter membrane.
The invention has the following beneficial effects:
according to the automatic enrichment and storage device for the environmental DNA samples, the filter membrane is fixed through the filter membrane fixing assembly, so that the thin and fragile filter membrane is integrated with the filter membrane fixing assembly, and the filter membrane is convenient to move and store; firstly, moving a sample processing component in a sample processing component storage unit into a sample storage unit through a conveying unit, then moving a filter membrane fixing component in a filter membrane fixing component storage unit onto a sample filtering unit through the conveying unit so as to enrich DNA samples in water samples on the filter membrane through pumping water samples, then moving the filter membrane fixing component on the sample filtering unit after completing sample enrichment onto the sample processing component in the sample storage unit through the conveying unit, and finally moving the sample processing component in the sample processing component storage unit onto the filter membrane fixing component in the sample storage unit through the conveying unit so as to process the filter membrane in the filter membrane fixing component, thereby realizing long-term sample preservation, and completing automatic enrichment of environmental DNA samples in multiple water samples and automatic storage of the environmental DNA samples in the filter membrane; the technical scheme realizes the automatic sampling and preservation of a plurality of environmental DNA samples by the mutual cooperative cooperation of the filter membrane fixing component, the filter membrane fixing component storage unit, the sample processing component storage unit, the sample filtering unit, the conveying unit and the sample storage unit, and compared with the prior art, the technical scheme eliminates the sample pollution problem possibly brought by manual operation, improves the environmental DND sampling enrichment and storage efficiency, meets the continuous automatic enrichment of the environmental DNA samples in a plurality of water samples and the continuous automatic preservation requirement of the environmental DNA samples in the filter membrane, has strong practicability, and is suitable for wide popularization and application.
In addition to the objects, features and advantages described above, the present invention has other objects, features and advantages. The present invention will be described in further detail with reference to the drawings.
Drawings
The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the invention. In the drawings:
FIG. 1 is a schematic diagram of an automatic enrichment and storage device for environmental DNA samples according to a preferred embodiment of the present invention;
FIG. 2 is an exploded schematic view of the automatic enrichment and storage device of the environmental DNA sample of FIG. 1;
FIG. 3 is a schematic view of a portion of the automated enrichment and storage device of the environmental DNA sample of FIG. 1;
FIG. 4 is an exploded schematic view of the automatic enrichment and storage device of the environmental DNA sample of FIG. 3;
FIG. 5 is a cross-sectional view of a filter immobilization assembly in an environmental DNA sample automatic enrichment and storage device according to a preferred embodiment of the present invention;
FIG. 6 is a cross-sectional view of a filter fixing assembly storage unit in an automatic enrichment and storage device for environmental DNA samples according to a preferred embodiment of the present invention;
FIG. 7 is a schematic diagram of the structure of a sample processing assembly in an automated ambient DNA sample enrichment and storage device in accordance with a preferred embodiment of the present invention;
FIG. 8 is a cross-sectional view of a sample processing assembly in an environmental DNA sample automatic enrichment and storage device, in accordance with a preferred embodiment of the present invention;
FIG. 9 is a cross-sectional view of a sample processing assembly storage unit in an environmental DNA sample automatic enrichment and storage device, in accordance with a preferred embodiment of the present invention;
FIG. 10 is a cross-sectional view of a sample storage unit in an environmental DNA sample automatic enrichment and storage device according to a preferred embodiment of the present invention;
FIG. 11 is a cross-sectional view showing a part of the structure of an automatic enrichment and storage device for an environmental DNA sample according to a preferred embodiment of the present invention.
Legend description:
1. a filter membrane fixing component; 11. a filter membrane; 12. an outer snap ring; 13. an inner snap ring; 2. a filter membrane fixing assembly storage unit; 21. a second lifting mechanism; 3. a sample filtration unit; 31. an inner sleeve; 32. a suction filter; 4. a sample storage unit; 5. a transfer unit; 51. a mounting frame; 52. a movable shaft; 53. a rotary driving member; 54. a clamping arm; 541. pressing the boss; 542. a sample inlet; 55. a positioning mechanism; 551. a dial; 552. positioning holes; 553. positioning the boss; 56. a moving driving member; 6. a sample processing assembly; 61. a disk-shaped main body; 62. drying the sheet; 7. a sample processing assembly storage unit; 71. a first lifting mechanism; 72. a movable claw.
Detailed Description
Embodiments of the invention are described in detail below with reference to the attached drawing figures, but the invention can be practiced in a number of different ways, as defined and covered below.
FIG. 1 is a schematic diagram of an automatic enrichment and storage device for environmental DNA samples according to a preferred embodiment of the present invention; FIG. 2 is an exploded schematic view of the automatic enrichment and storage device of the environmental DNA sample of FIG. 1; FIG. 3 is a schematic view of a portion of the automated enrichment and storage device of the environmental DNA sample of FIG. 1; FIG. 4 is an exploded schematic view of the automatic enrichment and storage device of the environmental DNA sample of FIG. 3; FIG. 5 is a cross-sectional view of a filter immobilization assembly in an environmental DNA sample automatic enrichment and storage device according to a preferred embodiment of the present invention; FIG. 6 is a cross-sectional view of a filter fixing assembly storage unit in an automatic enrichment and storage device for environmental DNA samples according to a preferred embodiment of the present invention; FIG. 7 is a schematic diagram of the structure of a sample processing assembly in an automated ambient DNA sample enrichment and storage device in accordance with a preferred embodiment of the present invention; FIG. 8 is a cross-sectional view of a sample processing assembly in an environmental DNA sample automatic enrichment and storage device, in accordance with a preferred embodiment of the present invention; FIG. 9 is a cross-sectional view of a sample processing assembly storage unit in an environmental DNA sample automatic enrichment and storage device, in accordance with a preferred embodiment of the present invention; FIG. 10 is a cross-sectional view of a sample storage unit in an environmental DNA sample automatic enrichment and storage device according to a preferred embodiment of the present invention; FIG. 11 is a cross-sectional view showing a part of the structure of an automatic enrichment and storage device for an environmental DNA sample according to a preferred embodiment of the present invention.
As shown in fig. 1 to 4, the automatic enrichment and storage device for an environmental DNA sample of the present embodiment includes: a filter fixing member storage unit 2 storing a plurality of filter fixing members 1 for filtering a water sample through a filter to extract a sample; the sample filtering unit 3 is used for carrying out sample enrichment by being matched with the filter membrane fixing component 1; a sample processing module storage unit 7 storing a plurality of sample processing modules 6 for processing samples to hold the samples; a sample storage unit 4 for storing the filter membrane fixing assembly 1 and/or the sample processing assembly 6; a transfer unit 5 for moving the filter membrane holder assembly 1 between the filter membrane holder assembly storage unit 2, the sample filtration unit 3 and the sample storage unit 4, and/or for moving the sample processing assembly 6 between the sample processing assembly storage unit 7 and the sample storage unit 4. Specifically, according to the automatic enrichment and storage device for the environmental DNA sample, the filter membrane is fixed through the filter membrane fixing component 1, so that the thin and fragile filter membrane 11 is integrated with the filter membrane fixing component 1, and the filter membrane 11 is convenient to move and store; firstly, moving a sample processing component 6 in a sample processing component storage unit 7 into a sample storage unit 4 through a conveying unit 5, then moving a filter membrane fixing component 1 in a filter membrane fixing component storage unit 2 onto a sample filtering unit 3 through the conveying unit 5 so as to enrich DNA samples in water samples on a filter membrane 11 through pumping water samples, then moving the filter membrane fixing component 1 which completes sample enrichment on the sample filtering unit 3 onto the sample processing component 6 in the sample storage unit 4 through the conveying unit 5, and finally moving the sample processing component 6 in the sample processing component storage unit 7 onto the filter membrane fixing component 1 in the sample storage unit 4 through the conveying unit 5 so as to process the filter membrane 11 in the filter membrane fixing component 1, thereby realizing long-term sample preservation, and completing automatic enrichment of environmental DNA samples in multiple water samples and automatic storage of environmental DNA samples in the filter membrane; the technical scheme realizes the automatic sampling and preservation of a plurality of environmental DNA samples by the mutual cooperative coordination of the filter membrane fixing component 1, the filter membrane fixing component storage unit 2, the sample processing component 6, the sample processing component storage unit 7, the sample filtering unit 3, the conveying unit 5 and the sample storage unit 4, and compared with the prior art, the automatic sampling and preservation method eliminates the sample pollution problem possibly brought by manual operation, improves the environmental DND sampling enrichment and storage efficiency, meets the continuous automatic enrichment of the environmental DNA samples in a plurality of water samples and the continuous automatic preservation requirement of the environmental DNA samples in the filter membrane 11, has strong practicability, and is suitable for wide popularization and application. Optionally, the filter fixing member storage unit 2, the sample filtration unit 3, the sample processing member storage unit 7 and the sample storage unit 4 are arranged at intervals, so that the filter fixing member 1 and/or the sample processing member 6 can be moved.
As shown in fig. 3 to 4, in this embodiment, the conveying unit 5 includes a mounting frame 51, a movable shaft 52 movably disposed on the mounting frame 51, a rotation driving member 53 disposed on the mounting frame 51 and connected to the movable shaft 52 for driving the movable shaft 52 to circumferentially rotate, a movement driving member 56 disposed on the mounting frame 51 and connected to the movable shaft 52 for driving the movable shaft 52 to axially move, and a clamping arm 54 fixed on the movable shaft 52 for clamping the filter membrane fixing assembly 1. Specifically, the movable shaft 52 is driven to vertically move by the moving driving member 56, so that the vertical height of the clamping arm 54 is higher than that of the filter membrane fixing assembly 1 to be clamped, mutual interference is avoided in motion, then the movable shaft 52 is driven to circumferentially rotate by the rotating driving member 53, so that the clamping end of the clamping arm 54 is located above the filter membrane fixing assembly 1, and then the movable shaft 52 is driven to vertically move by the moving driving member 56, so that the clamping end of the clamping arm 54 and the filter membrane fixing assembly 1 are correspondingly distributed, and the filter membrane fixing assembly 1 is clamped and moved, so that the movement and storage of the filter membrane 11 in the filter membrane fixing assembly 1 are realized. Optionally, the rotation driving member 53 includes a rotation driving structure disposed on the mounting frame 51, a rotation driving wheel fixedly sleeved on an output end of the rotation driving structure, a rotation driven wheel sleeved on the movable shaft 52, and a rotation driving belt connected to the rotation driving wheel and the rotation driven wheel respectively. It will be appreciated that the moveable shaft 52 is vertically moveable relative to the rotating driven wheel to move the clamp arm 54 vertically. Alternatively, the radial cross section of the movable shaft 52 is square, and the shape of the inner hole of the rotary driven wheel is matched with the radial cross section of the movable shaft 52. Optionally, the moving driving member 56 includes a moving driving structure disposed on the mounting frame 51, a moving screw rod vertically disposed on the mounting frame 51, a moving driving wheel fixedly sleeved on an output end of the moving driving structure, a moving driven wheel fixedly sleeved on the moving screw rod, a moving driving belt respectively connected with the moving driving wheel and the moving driven wheel, a moving screw rod sleeve sleeved on the moving screw rod, and a moving sleeve fixedly sleeved on the movable shaft 52 and fixedly connected with the moving screw rod sleeve, wherein the moving screw rod sleeve is in threaded connection with the moving screw rod. Optionally, the moving driving member 56 further includes two positioning rods vertically disposed on the mounting frame 51, the two positioning rods are respectively disposed on two opposite sides of the movable shaft 52, and a connecting sleeve fixedly connected with the moving screw sleeve and the moving sleeve is fixedly sleeved on the positioning rods, so that the movable shaft 52 is positioned through the positioning rods and the connecting sleeve, and the vertical movement of the movable shaft 52 is ensured to be stable. Optionally, the rotation driving structure is one of driving devices such as a motor, an air cylinder, an oil cylinder and the like. Preferably, the rotary drive structure is a motor. Optionally, the moving driving structure is one of driving devices such as a motor, an air cylinder, an oil cylinder and the like. Preferably, the movement driving structure is a motor. Optionally, the clamping end of the clamping arm 54 is provided with motorized clamping jaws for clamping or unclamping the filter membrane holder assembly 1. It should be appreciated that the specific structure of the motorized clasps is well known to those skilled in the art and will not be described in detail herein. Optionally, the clamping end of the clamping arm 54 is provided with a pressing boss 541 for pressing the filter membrane fixing assembly 1 and a sample inlet 542 which is axially arranged on the pressing boss 541 and is used for communicating with a water sample.
As shown in fig. 1 to 4, in this embodiment, the conveying unit 5 further includes a positioning mechanism 55 for determining a rotation position of the clamping end of the conveying unit 5 to ensure that the clamping arm 54 accurately clamps the filter membrane fixing assembly 1, the positioning mechanism 55 includes a dial 551 sleeved on the movable shaft 52, a plurality of scale grooves are disposed on the dial 551 and are in one-to-one correspondence with the filter membrane fixing assembly storage unit 2, the sample filtering unit 3 and the sample storage unit 4, and the positioning mechanism 55 further includes an induction controller electrically connected with the rotation driving member 53 for inducing the scale grooves to control the rotation of the rotation driving member 53 and thus control the rotation of the clamping arm 54. Specifically, the included angle between two adjacent scale grooves is the same as the included angle between two corresponding adjacent parts, that is, when the filter membrane fixing assembly 1 needs to be clamped and moved on the corresponding parts, for example, the sample filtering unit 3, the rotation driving piece 53 drives the rotation shaft to rotate, and then drives the clamping arm 54 to rotate, and accordingly drives the dial 551 to rotate, when the induction controller senses the scale grooves correspondingly distributed with the sample filtering unit 3, the rotation driving piece 53 is controlled to stop working, and at this time, the length direction of the clamping arm 54 is intersected with the axis line of the movable shaft 52 and the center line of the corresponding part respectively, so that the filter membrane fixing assembly 1 is accurately clamped. Optionally, the electrical connection comprises a wired electrical connection and a radio connection. Optionally, the sensing controller includes an infrared sensor and a PLC controller, and the infrared sensor is electrically connected to the PLC controller.
As shown in fig. 1 to 4, in this embodiment, the positioning mechanism 55 further includes a plurality of positioning holes 552 disposed on the mounting frame 51 and disposed in one-to-one correspondence with the filter membrane fixing assembly storage unit 2, the sample filtering unit 3 and the sample storage unit 4, and a positioning boss 553 disposed on the clamping arm 54 for inserting and matching with the positioning holes 552 to position the clamping arm 54. Specifically, the center line of the movable shaft 52, the center line of the positioning hole 552 and the center line of the corresponding component intersect in the same axis, that is, when the clamping arm 54 needs to clamp the filter membrane fixing assembly 1 on the corresponding component or move the filter membrane fixing assembly 1 on the corresponding component, the clamping end of the clamping arm 54 is moved to the upper side of the filter membrane fixing assembly 1 on the corresponding component by rotating the driving piece 53, and then the clamping arm 54 is driven to move vertically by the moving driving piece 56, at this time, the positioning boss 553 is in plug-in fit with the positioning hole 552, so as to ensure that the clamping end of the clamping arm 54 and the filter membrane fixing assembly 1 are coaxially arranged, and further the clamping arm 54 accurately clamps the filter membrane fixing assembly 1. It should be understood that, since the scale groove has a certain groove width, a certain error exists when the positioning of the clamping arm 54 is realized through the induction controller, so that the error is eliminated through the positioning hole 552 and the positioning boss 553, the clamping arm 54 is ensured to accurately clamp the filter membrane fixing assembly 1, the movement and the storage of the filter membrane 11 in the filter membrane fixing assembly 1 are realized, the design is ingenious, and the work is stable. Optionally, the end of the positioning boss 553 facing the positioning hole 552 is provided with a positioning cone surface, through which positioning precision is improved. Optionally, the maximum diameter of the locating boss 553 is the same as the aperture of the locating aperture 552 to ensure coaxial arrangement after the mating engagement.
As shown in fig. 1 to 4, in the present embodiment, the sample processing module 6 includes a disk-shaped body 61 for stacking connection with the filter fixing module 1 and a drying sheet 62 disposed on the disk-shaped body 61 for drying the filter to preserve the sample. Specifically, the disc-shaped main body 61 is in clamping fit with the filter membrane fixing component 1 so as to realize axial stacking, and then the filter membrane 11 is dried through the drying sheet, and then the plurality of sample processing components 6 stored in the sample processing component storage unit 7 can realize the drying of the filter membrane 11 in the plurality of filter membrane fixing components 1 in continuous automatic enrichment of environmental DNA samples in a plurality of water samples, the sample processing components 6 are clamped by the clamping arms 54 so that the two sample processing components 6 are respectively in clamping fit with the upper end and the lower end of the filter membrane fixing component 1, the drying and sealing preservation of the filter membrane 11 in the filter membrane fixing component 1 are realized, so that the environmental DNA sample preservation time is prolonged, the sample is prevented from being polluted, the authenticity and the accuracy of the subsequent sample detection result are influenced, namely, the sample processing components 6 and the filter membrane fixing component 1 are sequentially arranged in the sample storage unit 4, and the bottommost end and the topmost end are the sample processing components 6. It should be understood that, in the sample storage unit 4, the sample processing component 6 dries the filter membrane 11 in the filter membrane fixing component 1, and the sample processing component 6 in the filter membrane fixing component 1 and the sample processing component storage unit in which the enrichment of the environmental DNA sample has been completed in the sample filtering unit 3 is automatically transferred to the sample storage unit 4, and the sample processing component 6 is utilized to perform the sealed structural design on the filter membrane fixing component 1, so that the automatic preservation of the environmental DNA sample in the filter membrane under the normal temperature condition is realized, the sample pollution problem possibly caused by the manual operation is eliminated, the enrichment and storage efficiency of the environmental DNA sample is improved, and the continuous automatic preservation requirements of the environmental DNA sample in the filter membrane and the continuous automatic enrichment of the environmental DNA sample in a plurality of water samples are satisfied.
As shown in fig. 1 to 4, in the present embodiment, the sample processing component storage unit 7 includes a sample processing component storage cylinder for storing the sample processing components 6, and a first lifting mechanism disposed in the sample processing component storage cylinder for driving the sample processing components 6 to lift and lower axially, and a plurality of sample processing components 6 are stacked in the sample processing component storage unit 7 in the axial direction. Specifically, when the filter membrane fixing assembly 1 with the enrichment of the environmental DNA sample is required to be processed and stored, the first lifting mechanism 71 drives the sample processing assembly 6 in the sample processing assembly storage unit 7 to move upwards axially so as to eject the uppermost sample processing assembly 6 out of the cylinder opening. Optionally, a first end surface of the disc-shaped body 61 in the axial direction is convexly provided with a first annular clamping table, and a second end surface of the disc-shaped body 61 in the axial direction is convexly provided with a second annular clamping table, wherein the outer diameter of the first annular clamping table is smaller than the inner diameter of the second annular clamping table, or the outer diameter of the second annular clamping table is smaller than the inner diameter of the first annular clamping table, so that axial stacking of a plurality of sample processing assemblies 6 is realized, and the sample processing assemblies are conveniently stored in the sample processing assembly storage unit 7. Optionally, the first lifting mechanism 71 includes a first lifting screw rod arranged along an axial direction, a first driving structure with an output end connected with the first lifting screw rod, and a first lifting screw rod sleeve sleeved on the first lifting screw rod, and a first lifting platform fixedly connected with the first lifting screw rod sleeve and extending into the sample processing assembly storage unit 7, a first lifting groove for extending into the first lifting platform is formed in the side wall of the sample processing assembly storage barrel along the axial direction, and the sample processing assembly is axially stacked on the first lifting platform, and the first lifting screw rod sleeve is in threaded connection with the first lifting screw rod. Optionally, the first driving structure is one of driving devices such as a motor, an air cylinder, an oil cylinder and the like.
As shown in fig. 1 to 4, in this embodiment, the sample processing component storage unit 7 further includes movable claws 72 movably disposed in the radial direction of the sample processing component storage cylinder for being caught between two adjacent sample processing components 6 on the sample processing component storage cylinder so as to facilitate separation of the two adjacent sample processing components 6. Specifically, when the sample processing component 6 needs to be clamped, the movable claw 72 moves along the radial direction of the sample processing component storage unit 7 so as to be clamped between two adjacent sample processing components 6 on the sample processing component storage unit 7, the upper sample processing component 6 is clamped by the clamping arm 54, the movable claw 72 clamps the lower sample processing component 6, and the synchronous belt of the lower sample processing component 6 is avoided when the clamping arm 54 clamps the upper sample processing component 6, so that the separation of two adjacent sample processing components 6 at the cylinder opening of the sample processing component storage cylinder is facilitated.
As shown in fig. 11, in this embodiment, the sample filtering unit 3 includes a suction bottle, the mouth of the suction bottle is provided with an inner sleeve 31 extending into the bottle, a suction filter 32 for connecting a vacuum pump is provided on the body of the suction bottle, and the axial height of the suction filter 32 is higher than the axial height of the lower end of the inner sleeve 31. Specifically, the water sample is prevented from being sucked in by the vacuum pump after passing through the filter membrane 11 by the inner sleeve 31, thereby ensuring that the filtered water sample flows into the bottle bottom of the sample filter unit 3. Optionally, the bottle bottom of the sample filtering unit 3 is arranged at the water outlet, the water outlet is controlled to be automatically closed during suction filtration, and the water outlet is controlled to be automatically opened after the suction filtration is finished so as to discharge the water sample remained in the sample filtering unit 3. Optionally, the end face of the inner sleeve 31 facing the filter membrane 11 is provided with a net structure which is abutted with the filter membrane 11 and used for supporting the filter membrane 11, and the filter membrane 11 is supported by the net structure, so that the integrity of the filter membrane 11 is ensured, and the filter effect is prevented from being influenced by deformation or breakage of the filter membrane 11 under the action of pressure. Optionally, the outer wall surface of the sample filter unit 3 is engraved with graduation marks showing the capacity.
As shown in fig. 7 to 9, in this embodiment, the filter membrane fixing assembly storage unit 2 includes a filter membrane fixing assembly storage cylinder for storing filter membrane fixing assemblies, and a second lifting mechanism 21 disposed in the filter membrane fixing assembly storage cylinder for driving the filter membrane fixing assemblies 1 to lift axially, and a plurality of filter membrane fixing assemblies 1 are stacked in the filter membrane fixing assembly storage unit 2 along the axial direction. Specifically, when the water sample to be sampled needs to be filtered, the second lifting mechanism 21 drives the filter membrane fixing assembly 1 in the filter membrane fixing assembly storage unit 2 to move upwards in the axial direction so as to eject the filter membrane fixing assembly 1 at the uppermost end out of the cylinder opening. Optionally, the second lifting mechanism 21 includes a second lifting screw rod arranged along the axial direction, a second driving structure with an output end connected with the second lifting screw rod, and a second lifting screw rod sleeve sleeved on the second lifting screw rod, and a second lifting platform fixedly connected with the second lifting screw rod sleeve and extending into the filter membrane fixing assembly storage unit 2, a second lifting groove for extending into the second lifting platform is vertically formed in the side wall of the filter membrane fixing assembly storage barrel, the filter membrane assembly is axially stacked on the second lifting platform, and the second lifting screw rod sleeve is in threaded connection with the second lifting screw rod. Optionally, the second driving structure is one of driving devices such as a motor, an air cylinder, an oil cylinder and the like. Optionally, the filter membrane fixing component storage unit 2 and the sample processing component storage unit 7 are adjacently arranged, and the second lifting platform is arranged in a T shape and extends into the filter membrane fixing component storage unit 2 and the sample processing component storage unit 7 respectively so as to drive the filter membrane fixing component 1 and the sample processing component 6 to synchronously lift, at this time, the first lifting mechanism 21 and the second lifting mechanism 71 are the same component.
As shown in fig. 5 to 6, in this embodiment, the filter fixing assembly 1 further includes an outer collar 12 that supports the lower end surface of the filter 11, and an inner collar 13 that is attached to the inner wall surface of the outer collar 12 and presses against the upper end surface of the filter 11. Specifically, the lower terminal surface of outer clamping ring 12 support filter membrane 11, the laminating of inner clamping ring 13 and the inner wall face of outer clamping ring 12 is supported the up end of pressing filter membrane 11 to hold filter membrane 11 fixedly, through the close fit of inner clamping ring 13 and outer clamping ring 12, fix filter membrane 11 in filter membrane fixed subassembly 1, form a rigid whole, the centre gripping of the fixed subassembly 1 of filter membrane of being convenient for shifts, still can avoid filter membrane 11 to take place to remove and reduce the filterable effect in the sample filtration process, thereby influence accuracy and the reliability of sample testing result. The outer clamping ring 12 and the inner clamping ring 13 in the filter membrane fixing assembly 1 are arranged in a detachable clamping manner, so that the filter membrane is convenient to take and place, the filter membrane fixing assembly 1 can be recycled, and resource waste is avoided. Optionally, the inner wall of the outer clamping ring 12 is provided with a first circular clamping table which is formed by extending inwards in the radial direction and is used for supporting the lower end face of the filter membrane 11, the first circular clamping table is provided with a filter hole, and the filter membrane is supported by the first circular clamping table, so that the filter membrane 11 is prevented from being deformed or damaged under the action of pressure to influence the filtering effect. Preferably, the inner wall of the outer clamping ring 12 is provided with a first annular clamping table which is formed by extending inwards in the radial direction and is used for supporting the lower end face of the filter membrane 11, when water sample filtration is carried out, the lower end face of the filter membrane 11 is contacted with the supporting surface of the reticular structure through the cooperation of the first annular clamping table and the reticular structure arranged on the upper opening of the water sample receiving container, so that the filter membrane 11 is prevented from being deformed or damaged under the action of pressure to influence the filtration effect; when the water sample is filtered, the sample is required to be stored and the filter membrane component is required to be transferred, the contact surface between the first annular clamping table which supports the filter membrane and the lower surface of the filter membrane is smaller, the adhesion of water on the filter membrane can be reduced, the drying treatment effect of the filter membrane 11 in the filter membrane fixing component 1 after the filtration is improved, and the sample storage is facilitated. Optionally, the outer wall of the outer collar 12 remote from the first annular collar/first circular collar end is provided with a second annular collar extending radially outwardly therefrom, the outer collar 12 and the second annular collar cooperating to facilitate gripping by the gripping arms 54. Optionally, the end face of the second annular clamping table, which faces away from the outer clamping ring 12, is provided with a third annular clamping table formed by extending along the axial direction, and the inner diameter of the third annular clamping table is larger than the outer diameter of the outer clamping ring 12, so that axial stacking of a plurality of filter membrane fixing assemblies 1 is realized, and the filter membrane fixing assemblies are conveniently stored in the filter membrane fixing assembly storage unit 2. Optionally, when the outer diameter of the first annular clamping table is smaller than the inner diameter of the second annular clamping table, the inner diameter of the first annular clamping table is larger than the outer diameter of the outer clamping ring 12, and the inner diameter of the second annular clamping table is larger than the outer diameter of the third annular clamping table, so that the axial stacking of the sample processing assembly 6 and the filter membrane fixing assembly 1 is realized, the sample is conveniently stored in the sample storage unit 4, and the sealing preservation of the filter membrane 11 in the filter membrane fixing assembly 1 is realized.
As shown in fig. 3, 4 and 10, the sample storage unit 4 in this embodiment includes a sample storage cylinder and a third lifting mechanism disposed in the sample storage cylinder for driving the filter membrane fixing assembly 1 and/or the sample processing assembly 6 to lift axially, and the plurality of filter membrane fixing assemblies 1 and the plurality of sample processing assemblies 6 are stacked in the sample storage cylinder in sequence. Optionally, the third elevating system includes the third lift lead screw that lays along the axial, the output is connected with the third drive structure of third lift lead screw and the cover is located the third lift lead screw on the third lift lead screw and is overlapped the third lift platform that locates third lift lead screw cover fixed connection and stretch into in the third storage unit 4, the lateral wall of sample storage barrel is along vertical the offering be used for the third lift platform to stretch into the third lift groove, sample processing assembly 6 and the filter membrane fixed subassembly 1 that has accomplished the enrichment of environment DNA sample are alternately axial stack in proper order on third lift platform, third lift lead screw cover and third lift lead screw threaded connection. Optionally, the automatic enrichment and storage device for the environmental DNA sample comprises two sample storage units 4, the two sample storage units 4 are adjacently arranged, and the third lifting platform is arranged in a T shape and respectively stretches into the two sample storage cylinders so as to drive the samples in the two sample storage units 4 to synchronously lift.
As shown in fig. 1, in this embodiment, the automatic enrichment and storage device for environmental DNA samples further includes a fixing base, and the filter membrane fixing component storage unit 2, the sample processing component storage unit 7, the sample storage unit 4, and the transmission unit 5 are disposed on the fixing base, so that the relative positions among the filter membrane fixing component storage unit 2, the sample processing component storage unit 7, the sample storage unit 4, and the transmission unit 5 are fixed, thereby facilitating automatic membrane changing, sample collection, and storage.
As shown in fig. 1-11, the specific working steps of the automatic enrichment and storage device for environmental DNA samples are as follows: 1. the first lifting mechanism 71 drives the first lifting platform in the sample processing component storage unit 7 to move upwards so as to push the uppermost sample processing component 6 in the sample processing component storage unit 7 out of the barrel opening of the sample processing component storage unit 7, the rotary driving piece 53 and the movable driving piece 56 drive the clamping arm 54 to move to the position above the sample processing component storage unit 7 and clamp the sample processing component 6, then the sample processing component 6 is moved to the barrel opening of the sample storage unit 4 so as to place the sample processing component 6 on the third lifting platform in the sample storage unit 4, and the third lifting mechanism drives the sample processing component 6 to move downwards so that the upper end face of the sample processing component 6 is level with the upper end face of the barrel opening of the sample storage unit 4; 2. the second lifting mechanism 21 drives the second lifting platform in the filter membrane fixing component storage unit 2 to move upwards so as to eject the filter membrane fixing component 1 at the uppermost end in the filter membrane fixing component storage unit 2 out of the cylinder mouth of the filter membrane fixing component storage unit 2, the rotary driving piece 53 and the movable driving piece 56 drive the clamping arm 54 to move to the position above the filter membrane fixing component storage unit 2 and clamp the filter membrane fixing component 1, then the filter membrane fixing component 1 is moved to the position above the sample filtering unit 3, the filter membrane fixing component 1 is placed at the bottle mouth of the sample filtering unit 3, the first end face of the filter membrane 11 is in contact with the supporting surface of the net structure, and finally the filter membrane fixing component 1 is loosened; 3. the movable driving piece 56 drives the clamping arm 54 to move downwards, so that the pressing boss 541 presses against the filter membrane fixing assembly 1, and the water outlet end of the sample inlet 542 is tightly attached to the upper surface of the filter membrane 11; 4. the vacuum pump works to extract the water sample to be sampled through the sample inlet 542, and the water sample enters from the sample inlet 542 and is filtered by the filter membrane 11; 5. after the suction filtration is completed, the rotation driving piece 53 and the moving driving piece 56 drive the clamping arm 54 to clamp the filter membrane fixing assembly 1 and move to the upper part of the sample storage unit 4, at the moment, the third lifting mechanism drives the third lifting platform to move upwards so as to fasten the sample processing assembly 6 in the sample storage unit 4 with the lower half part of the filter membrane fixing assembly 1, and then the third lifting mechanism drives the third lifting platform to move downwards so as to enable the filter membrane fixing assembly 1 to enter the sample storage unit 4; 6. and (3) repeating the step (1), fastening the next sample processing assembly 6 on the upper half part of the filter membrane fixing assembly 1, and repeating the cycle, so as to realize continuous automatic enrichment of the environmental DNA samples in the water samples and continuous automatic preservation of the environmental DNA samples in the filter membrane.
The above description is only of the preferred embodiments of the present invention and is not intended to limit the present invention, but various modifications and variations can be made to the present invention by those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (10)
1. An automated environmental DNA sample enrichment and storage device, comprising:
a filter membrane fixing assembly storage unit (2) storing a plurality of filter membrane fixing assemblies (1) for filtering a water sample through a filter membrane to extract the sample;
the sample filtering unit (3) is used for carrying out sample enrichment by being matched with the filter membrane fixing component (1);
a sample processing module storage unit (7) storing a plurality of sample processing modules (6) for processing a sample to store the sample;
a sample storage unit (4) for storing the filter membrane fixing assembly (1) and/or the sample processing assembly (6);
and a transfer unit (5) for moving the filter membrane holder assembly (1) between the filter membrane holder assembly storage unit (2), the sample filtration unit (3) and the sample storage unit (4), and/or for moving the sample processing assembly (6) between the sample processing assembly storage unit (7) and the sample storage unit (4).
2. The automatic enrichment and storage device for environmental DNA samples according to claim 1, wherein the transfer unit (5) comprises a mounting frame (51), a movable shaft (52) movably arranged on the mounting frame (51), a rotation driving member (53) which is arranged on the mounting frame (51) and connected with the movable shaft (52) and is used for driving the movable shaft (52) to rotate circumferentially, a movement driving member (56) which is arranged on the mounting frame (51) and connected with the movable shaft (52) and is used for driving the movable shaft (52) to move vertically, and a clamping arm (54) which is fixed on the movable shaft (52) and is used for clamping the filter membrane fixing assembly (1).
3. The automatic enrichment and storage device for environmental DNA samples according to claim 2, wherein the transfer unit (5) further comprises a positioning mechanism (55) for determining the rotation position of the clamping end of the transfer unit (5) so as to ensure that the clamping arm (54) accurately clamps the filter membrane fixing assembly (1), the positioning mechanism (55) comprises a dial (551) sleeved on the movable shaft (52), a plurality of scale grooves which are arranged in one-to-one correspondence with the filter membrane fixing assembly storage unit (2), the sample filtering unit (3) and the sample storage unit (4) are arranged on the dial (551), and the positioning mechanism (55) further comprises an induction controller which is electrically connected with the rotation driving member (53) and is used for inducing the scale grooves so as to control the operation of the rotation driving member (53) and further control the rotation of the clamping arm (54).
4. The automatic enrichment and storage device for environmental DNA samples according to claim 3, wherein the positioning mechanism (55) further comprises a plurality of positioning holes (552) which are arranged on the mounting frame (51) and are arranged in one-to-one correspondence with the filter membrane fixing assembly storage unit (2), the sample filtering unit (3) and the sample storage unit (4), and positioning bosses (553) which are arranged on the clamping arms (54) and are used for being in plug-in fit with the positioning holes (552) to position the clamping arms (54).
5. The automatic enrichment and storage device for environmental DNA samples according to any of the claims 1-4, wherein the sample handling assembly (6) comprises a disc-shaped body (61) for stacking connection with the filter fixing assembly (1) and a drying plate arranged on the disc-shaped body (61) for drying the filter for preserving the sample.
6. The automatic enrichment and storage device for environmental DNA samples according to any of claims 1 to 4, wherein the sample processing module storage unit (7) comprises a processing module storage cylinder for storing the sample processing modules (6) and a first lifting mechanism arranged in the processing module storage cylinder for driving the sample processing modules (6) to lift up and down axially, and a plurality of sample processing modules (6) are stacked in the sample processing module storage unit (7) in the axial direction.
7. The automatic enrichment and storage device for environmental DNA samples according to claim 6, wherein the sample processing component storage unit (7) further comprises movable claws (72) movably arranged in a radial direction of the sample processing component storage cylinder for being caught between two adjacent sample processing components (6) on the sample processing component storage cylinder so as to separate the two adjacent sample processing components (6).
8. The automatic enrichment and storage device for environmental DNA samples according to any of the claims 1-4, wherein the sample filtering unit (3) comprises a suction bottle, the mouth of the suction bottle is provided with an inner sleeve (31) extending inwards, the body of the suction bottle is provided with a suction filter (32) for connecting a vacuum pump, and the axial height of the suction filter (32) is higher than the axial height of the lower end of the inner sleeve (31).
9. The automatic enrichment and storage device for environmental DNA samples according to any of claims 1 to 4, wherein the filter fixing assembly storage unit (2) comprises a filter fixing assembly storage cylinder for storing filter fixing assemblies and a second lifting mechanism (21) which is arranged in the filter fixing assembly storage cylinder and is used for driving the filter fixing assemblies (1) to axially lift, and a plurality of filter fixing assemblies (1) are axially stacked in the filter fixing assembly storage unit (2).
10. The automatic enrichment and storage device for environmental DNA samples according to any of claims 1-4, wherein the filter membrane fixing assembly (1) further comprises an outer clamping ring (12) which is attached to the lower end surface of the support filter membrane (11) and an inner clamping ring (13) which is attached to the inner wall surface of the outer clamping ring (12) and abuts against the upper end surface of the filter membrane (11).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210697027.5A CN117286019A (en) | 2022-06-20 | 2022-06-20 | Automatic enrichment and storage device for environmental DNA samples |
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| CN202210697027.5A CN117286019A (en) | 2022-06-20 | 2022-06-20 | Automatic enrichment and storage device for environmental DNA samples |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117736841A (en) * | 2024-02-21 | 2024-03-22 | 青岛市动物疫病预防控制中心 | Bacterial stock solution treatment device for bacterial drug resistance detection and analysis and use method thereof |
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2022
- 2022-06-20 CN CN202210697027.5A patent/CN117286019A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117736841A (en) * | 2024-02-21 | 2024-03-22 | 青岛市动物疫病预防控制中心 | Bacterial stock solution treatment device for bacterial drug resistance detection and analysis and use method thereof |
| CN117736841B (en) * | 2024-02-21 | 2024-05-03 | 青岛市动物疫病预防控制中心 | Bacterial stock solution treatment device for bacterial drug resistance detection and analysis and use method thereof |
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