CN219441261U - Automatic fungus collecting device - Google Patents

Automatic fungus collecting device Download PDF

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Publication number
CN219441261U
CN219441261U CN202320352533.0U CN202320352533U CN219441261U CN 219441261 U CN219441261 U CN 219441261U CN 202320352533 U CN202320352533 U CN 202320352533U CN 219441261 U CN219441261 U CN 219441261U
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assembly
filter
pipeline
driving
positioning
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CN202320352533.0U
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Chinese (zh)
Inventor
高凯斐
刘兴海
徐磊
杨胜强
李夏
葛焕飞
沈志林
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Zhejiang Tailin Life Science Co ltd
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Zhejiang Tailin Life Science Co ltd
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Abstract

The utility model discloses an automatic bacteria collecting device which comprises a filtering assembly, a peristaltic pump assembly, a driving assembly, a sample bottle clamp and a rack. The device comprises a filter assembly and a bacteria collecting frame for placing the filter assembly, wherein a first pipeline between a needle head and a filter cup body forms a first pipeline group, the bacteria collecting frame is used for installing and positioning the filter cup body of the filter assembly, a rack for locating and installing a sample bottle clamp, a driving assembly and the filter assembly to be matched with each other and the peristaltic pump assembly to be opposite to each other is arranged, the peristaltic pump is driven by the driving assembly, so that a roller assembly of the peristaltic pump is matched with a clamping block to be matched with the first pipeline group positioned at a rack placing platform and the filter assembly, and the sample liquid of a sample bottle is pumped into each filter cup body of the filter cup body, so that the automation of the filtration of the filter cup body is realized; finally, the sealing and the culture of the filter cup body can be completed through the clamping jaw. The peristaltic pump assembly can enable the automatic bacteria collecting device to be used independently. The use of the automated bacterial harvesting device is no longer limited by the air supply.

Description

Automatic fungus collecting device
Technical Field
The utility model relates to an automatic bacteria collecting device.
Background
Sterility testing is a test that checks whether a sample contains viable microorganisms that is not an alternative to a sterile product. According to the relevant legal requirements, the sterility test requires the filtration of the test sample or its solution or eluate in several parallel membrane filters which are closed and the cultivation of the microorganisms on the filter membranes. The existing sterile inspection adopts a peristaltic pump for driving, a hose is required to be installed and complex operation is required to be carried out for many times, the whole process is required to be completed through personnel operation, the labor intensity is high, the operation reproducibility is poor, and the control and the management of the inspection process are not facilitated.
The existing fungus collecting device needs an air source to achieve liquid taking, and the automatic fungus collecting device cannot be separated from the air source to work independently, so that the application range of the automatic fungus collecting device is limited, and great inconvenience is caused to use.
Disclosure of Invention
The utility model provides an automatic bacteria collecting device which can realize the process automation of sterile inspection, liberate operators, break through the filtration efficiency limit of the traditional mode, improve the working efficiency, and can be independently operated without an air source.
The technical scheme for solving the existing problems is as follows: an automatic bacteria collecting device comprises a filtering component, a plurality of clamping jaws capable of clamping a closed pipeline, a peristaltic pump component, a driving component, a sample bottle clamp and a frame.
The filter assembly comprises a filter cup group and a needle head, wherein the filter cup group comprises at least one filter cup body, the filter cup group is provided with a first air filter, the needle head is provided with a second air filter, a first pipeline is communicated between the needle head and each filter cup body, the first pipeline between the needle head and the filter cup body forms a first pipeline group, and the filter assembly is provided with a fungus collecting frame for placing and installing the filter assembly.
The peristaltic pump assembly is used for being matched with the first pipeline group and pumping sample liquid into the filter cup body, the peristaltic pump assembly is arranged at the corresponding position of the bacteria collecting frame, and the peristaltic pump assembly comprises a roller assembly and a clamping block which is matched with the roller assembly and clamps the first pipeline group; the first pipeline group is at least positioned at the position matched with the roller assembly and is a hose.
The driving assembly comprises one of a driving roller assembly and a clamping block, and a first driving structure matched with the other driving structure arranged on the bacteria collecting frame or the rack; or the driving assembly comprises a first driving structure for driving the roller assembly and a second driving structure for driving the clamping block, and the first driving structure and the second driving structure drive the roller assembly to be matched with the clamping block.
The rack is used for positioning and installing the sample bottle clamp, the driving assembly and the relative position of the filter assembly, and the rack is provided with a positioning station for positioning and placing the filter assembly.
As a further improvement, the clamping jaw is a manipulator which is arranged at a corresponding position on the frame and can clamp a closed pipeline.
The filter device is characterized by further comprising a bottom frame, wherein the frame comprises a first frame sliding on the bottom frame, a second frame which is arranged in a relatively fixed manner, a positioning station for positioning and placing the filter assembly is arranged on the first frame, and a driving assembly and clamping jaws are arranged on the second frame.
As a further improvement, a positioning clamp for positioning and clamping the bacteria collecting frame of the filter assembly is arranged on the positioning station for positioning and placing the filter assembly; the bacteria collecting frame is provided with a through hole matched with the clamping block by the roller assembly at the peristaltic pump assembly.
As a further improvement, the first frame is provided with a third driving structure for driving the first frame to slide.
As a further improvement, the positioning clamp is provided with a fourth driving structure for driving the positioning clamp to open and clamp.
As a further improvement, two needles are arranged, and each filter cup body is communicated with each needle head and a first pipeline between the two needles; the sample bottle clamp is provided with two groups.
As a further improvement, the sample bottle clamp is arranged above the bacteria collecting frame. The sample bottle clamp can rotate up and down by 180 degrees.
As a further improvement, the filter device also comprises a pipeline, wherein one end of the pipeline is connected with a first pipeline on each filter cup body, each needle is provided with a needle pipeline, and the other end of the pipeline is connected and communicated with each needle pipeline.
As a further improvement, a first conversion head is arranged between the parallel pipeline and the needle head pipeline, and a second conversion head is arranged between the pipeline and the first pipeline.
As a further improvement, the clamping block is provided with a concave cambered surface matched with the roller assembly.
As a further improvement, the bottom of the fungus collecting frame is provided with a liquid discharge groove.
As a further improvement, each filter cup is provided with a first air filter.
As a further improvement, a second air filter is arranged on each needle.
As a further improvement, the device also comprises a limiting hoop which is arranged on the bacteria collecting frame and used for positioning the filtering cup body; the bacteria collecting frame is provided with a protective cover plate for installing and positioning the filter cup group, and the protective cover plate is provided with a fixing structure for fixing the protective cover plate.
Compared with the prior art, the utility model is provided with the filter assembly and the bacteria collecting frame for placing the filter assembly, the first pipeline between the needle head and the filter cup body forms a first pipeline group, the bacteria collecting frame is used for installing and positioning the filter cup body of the filter assembly, and is provided with the rack for locating and installing the sample bottle clamp, the driving assembly is matched with the filter assembly and the relative position of the peristaltic pump assembly, and the roller assembly of the peristaltic pump is matched with the clamping block through the driving assembly so as to realize the matching with the first pipeline group positioned at the rack placing platform and the filter assembly, thus realizing the filtration automation of the sample liquid of the sample bottle into each filter cup body of the filter cup body; finally, the sealing and the culture of the filter cup body can be completed through the clamping jaw. The peristaltic pump assembly can enable the automatic bacteria collecting device to be used independently. The use of the automated bacterial harvesting device is no longer limited by the air supply.
Drawings
Fig. 1 is a schematic structural view of the present utility model.
Fig. 2 is a side view of the present utility model.
Fig. 3 is a schematic view of another angle of the present utility model.
Fig. 4 is an enlarged schematic view at a of fig. 1.
Fig. 5 is a schematic view of the structure of the filter assembly of the present utility model.
Fig. 6 is a schematic view of the structure of the first pipe group of the filter assembly.
FIG. 7 is a schematic view of the configuration of the clamp block, roller assembly and first tube set in cooperation.
Fig. 8 is a schematic view of the structure of the filter bowl of the present utility model.
Detailed Description
Referring to fig. 1-8, an automatic bacteria collecting device according to this embodiment includes: a filter assembly, a peristaltic pump assembly 2, a driving assembly, a frame 10 and a sample bottle clamp 11.
The filter assembly comprises a filter cup group and a needle head 4, wherein the filter cup group comprises at least one filter cup body 3, the filter cup group is provided with a first air filter 31, the needle head 4 is provided with a second air filter 42, a first pipeline 5 is communicated between the needle head 4 and each filter cup body 3, and the first pipeline 5 between the needle head 4 and the filter cup body 3 forms a first pipeline group; the filter assembly is provided with a bacteria collecting frame 1 for placing and installing the filter assembly; the number of the filter cups 3 is usually 1-4, in this embodiment, three filter cups are taken as an example, and the specific number of the filter cups is determined according to actual needs.
A plurality of clamping jaws 12 which clamp closed the pipeline. The jaws 12 may be provided with a fusing function. During pumping, the corresponding lines may be closed by the jaws 12, as desired. The jaws 12 may be of prior art construction, the specific number of which may be set according to the filtration requirements. In this embodiment, the clamping jaw 12 is a manipulator which is arranged on the frame 10 at a corresponding position and can clamp a closed pipeline, and the manipulator can be driven by a finger cylinder.
The bacteria collecting frame 1 is used for placing and installing a filter assembly, and a shell can be arranged on the bacteria collecting frame. For ease of use, sample bottle holder 11 may also be included; the sample bottle clamp 11 is arranged above the bacteria collecting frame 1 and is arranged on the frame 10.
Peristaltic pump subassembly 2 is used for pumping into the sample liquid in the filtration cup 3, peristaltic pump subassembly 2 set up in album fungus frame corresponding place. The peristaltic pump assembly 2 comprises a roller assembly 22 and a clamping block 21 which is matched with the roller assembly 22 and clamps the first pipeline group. The roller assembly 22 includes upper and lower covers, and a plurality of rollers which are clamped between the upper and lower covers and can be matched with the clamping blocks 21. The rollers are uniformly distributed on the circumferences of the upper cover body and the lower cover body. The upper cover body or the lower cover body is provided with a driving motor for driving the upper cover body and the lower cover body to rotate, and the rotation axis is the axis of the circumference; the drive motor may be internal or external. The rotating upper cover body and the rotating lower cover body drive the rollers uniformly distributed on the circumference of the ring to rotate so as to periodically drive the first pipeline group between the pressing and clamping blocks 21, and the first pipeline group is matched with the first pipeline group of the filter assembly placed at the placing station 8 of the frame 10 through the roller assemblies 22. As a power source for pumping liquid into the filter cup in cooperation with the first tubing set. The first tubing set and peristaltic pump assembly 2 may be constructed in accordance with known techniques. The first pipeline group is at least positioned at the position matched with the roller assembly and is a hose.
The driving assembly comprises one of a driving roller assembly 22 and a clamping block 21, and a first driving structure 7 which is matched with the other driving structure arranged on the bacteria collecting frame 1 or the frame 10;
or the driving component comprises a first driving structure 7 for driving the roller assembly 22 and a second driving structure for driving the clamping block 21, and the first driving structure 7 and the second driving structure drive the roller assembly to be matched with the clamping block. The first driving structure and the second driving structure can be driven by adopting the prior art such as a linear motor, an air cylinder, a ball screw with driving force, a screw rod hydraulic cylinder and the like.
The rack 10 is used for positioning and installing the sample bottle clamp 11, the relative position of the driving assembly and the matching of the filtering assembly, and the rack 10 is provided with a placing station 8 for positioning and placing the filtering assembly.
In this embodiment, the roller assembly 22 is preferably disposed on one side of the frame 10 on the filter assembly, and the first driving structure 7 is disposed on the other side of the frame 10 on the filter assembly.
As other embodiments, it is of course also possible to mount the roller assembly 22 and/or the clamping block 21 on the bacteria collecting frame, and drive the roller assembly 22 or the clamping block 21 by the first driving structure 7 of the driving assembly mounted on the frame 10 so as to be matched with the hose portion of the first pipeline group.
Of course, the clamping block 21 and/or the rotating wheel assembly 22 can also be mounted on an external bracket, and the clamping block 21 and the rotating wheel assembly 22 can be matched with the hose portion of the first pipeline group for use through the external bracket, and even an external power assembly can be further arranged to drive the extrusion clamping block 21 and the rotating wheel assembly 22 to clamp the hose portion and match with the hose portion.
For convenient use, the machine frame 10 further comprises a bottom frame 103, the machine frame 10 comprises a first machine frame 101 sliding on the bottom frame 103, a second machine frame 102 which is fixedly arranged relatively, a placing station 8 for positioning and placing the filter assembly is arranged on the first machine frame 101, and a driving assembly and clamping jaws are arranged on the second machine frame 102. Referring to the drawings, in this embodiment, the sample bottle holder 11 is also mounted on the first frame 101.
The positioning fixture 81 for positioning and clamping the filter assembly bacteria collecting frame 1 is arranged on the positioning station 8 for positioning and placing the filter assembly; the bacteria collecting frame 1 is provided with a through hole 24 where a roller assembly 22 is matched with the clamping block 21 at the peristaltic pump assembly.
The first rack 101 is provided with a third driving structure for driving the first rack 101 to slide; the positioning clamp 81 may include a pair of clamping plates that clamp the filter assembly. The positioning clamp 81 is provided with a fourth driving structure 82 for driving the positioning clamp 81 to open and clamp, and the fourth driving structure 82 can drive a clamping plate or a pair of clamping plates to position and clamp the filter assembly.
The second, third and fourth driving structures may be similar to the first driving structure 7.
For continuous operation, the needles 4 are provided with two, and each filter cup 3 is communicated with a first pipeline 5 between each needle 4 and the filter cup; the sample bottle clamp 11 is provided with two groups.
The sample bottle clamp 11 can rotate up and down by 180 degrees, and can rotate manually or through motor power. The sample bottle clamp 11 can be installed on the bacteria collecting frame 1, and can also be installed on the external bracket 1 to keep a matched relationship with the bracket 1 so as to facilitate sampling.
In order to simplify the connection, the filter cup further comprises a parallel pipeline 6, one end of the parallel pipeline 6 is connected with the first pipeline 5 on each filter cup 3, each needle 4 is provided with a needle pipeline 41, and the other end of the parallel pipeline 6 is connected with and communicated with each needle pipeline 41.
The first conversion head 61 is arranged between the parallel pipeline 6 and the needle pipeline 41, the first conversion head 61 can be a tee joint, the second conversion head 62 is arranged between the pipeline 6 and the first pipeline 5, and the second conversion head 62 can be a four-way joint.
The clamp block 21 is provided with a concave cambered surface 23 matched with the roller assembly 22. The bottom of the bacteria collecting frame 1 is provided with a liquid discharge groove.
In this embodiment, each filter bowl 3 is provided with a first air filter 31. A second air filter 42 is provided on each needle 4.
The utility model also comprises a limiting hoop 14 which is arranged on the bacteria collecting frame 1 and used for positioning the filtering cup body 3. The limit hoop 14 can be convenient for the filter cup body 3 to be fast clamped into the fungus collecting frame 1, and the fungus collecting frame 1 can be provided with a protective cover plate for installing and positioning the filter cup group. The protection cover plate is provided with a fixing structure for fixing the protection cover plate, the fixing structure can be an electromagnet arranged on the outer side of the bacteria collecting frame 1, and a bent arm clamping column for fastening the protection cover plate is arranged on the electromagnet.
Referring to the drawings, in this embodiment, the filter assembly of the bacteria collecting rack 1 provided with the filter cup 3 is installed and placed during operation, and the filter cup 3 can be installed on the bacteria collecting rack 1 usually through the base 32. The placing station 8 of the frame 10, that is, the placing station 8 of the first frame 101 in this embodiment, makes the hose portion of the first pipeline group of the filter assembly cooperate with the roller assembly 22 located on the frame, then the filter assembly can be positioned by the positioning fixture 81, and the filter assembly can be positioned by driving the positioning fixture 81 through the fourth driving structure of the positioning fixture 81. Then, the first driving structure 7 of the driving assembly drives the clamping block 21 to be matched with the roller assembly 22 to clamp the hose part of the first pipeline group.
One sample bottle may be mounted in one of the sample bottle holders 11 as desired, or preferably two sample bottles are mounted in each of the two sets of sample bottle holders 11. Any needle can be inserted as a first needle into the rubber plug of a sample bottle, typically placed upside down on the sample bottle holder 11, either manually or by a separate external manipulator. The jaw 12 is first used to close the needle line of another needle, which acts as a second needle. The peristaltic pump assembly 2 is started, the peristaltic pump assembly 2 starts to work, and the sample liquid of the sample bottle connected with the first needle is transferred and pumped into the three filtering cups 3, and filtering is started. Before the liquid in the sample bottle inserted by the first needle is pumped out, the second needle is inserted into the rubber plug of another sample bottle, and the sample liquid in the sample bottle inserted by the second needle is transferred and pumped into the three filter cups 3. The clamping jaw 12 on the needle pipe of the second needle is opened, the needle pipe of the first needle is closed by the clamping jaw 12, and the continuous pumping of the sample liquid into the filter cup body 3 is realized by analogy. The filtered liquid is discharged through the drain pipe of the filter bowl 3, and microorganisms are trapped on the filter membrane.
In order to facilitate the insertion of the needle, the sample bottle with the upward rubber plug can be placed in the sample bottle clamp 11 in advance, and after the needle is inserted, the sample bottle clamp 11 is rotated again to invert the sample bottle, so that the rubber plug end is downward, and the sample liquid can be conveniently transferred into the filter cup body 3.
The above operation is repeated to complete the filtration of all samples, then the lower drain pipe of each filter cup 3 is closed by the clamping jaw 12, meanwhile, two needle pipelines are closed by the clamping jaw 12, the sample liquid bottle is replaced by a bottle filled with culture medium, the first needle is inserted into a culture medium bottle, the needle pipeline of the first needle is opened, the first pipeline of one filter cup 3 is reserved to be opened, the first pipelines of the other filter cups 3 are closed by the clamping jaw 12, and the culture medium is transferred into one filter cup 3 with the first pipeline unblocked by the peristaltic pump assembly 2. The first and second needle operations in transferring the culture medium can refer to the sample liquid transferring process. Repeating the above operation, and sequentially filling the culture medium into the other residual filter cup bodies 3 until the filling of the culture medium in all the filter cup bodies 3 is completed. The first line and the air line of the three filter cups 3 are then closed by the clamping jaws 12, as shown in fig. 8. The three filter cups 3 form a fully-closed special culture container, and can be transferred to different temperatures for culture to determine whether the sample contains microorganisms. If necessary, the next filter element can be replaced continuously to sample and collect bacteria at the placement station 8.
If a needle is used, a needle is used to pump the liquid and medium into the filter bowl 3 in accordance with normal operation.

Claims (14)

1. An automatic change collection fungus device, its characterized in that: the filter assembly comprises a filter cup group and a needle head, wherein the filter cup group comprises at least one filter cup body, the filter cup group is provided with a first air filter, the needle head is provided with a second air filter, a first pipeline is communicated between the needle head and each filter cup body, the first pipeline between the needle head and the filter cup body forms a first pipeline group, and the filter assembly is provided with a bacteria collecting frame for placing and installing the filter assembly; a plurality of clamping jaws capable of clamping the closed pipeline; the peristaltic pump assembly is used for being matched with the first pipeline group and pumping sample liquid into the filter cup body, the peristaltic pump assembly is arranged at the corresponding position of the bacteria collecting frame, and the peristaltic pump assembly comprises a roller assembly and a clamping block which is matched with the roller assembly and clamps the first pipeline group; the first pipeline group is at least positioned at the position matched with the roller assembly and is a hose; the driving assembly comprises one of a driving roller assembly and a clamping block, and a first driving structure matched with the other driving structure arranged on the bacteria collecting frame or the rack; or the driving assembly comprises a first driving structure for driving the roller assembly and a second driving structure for driving the clamping block, and the first driving structure and the second driving structure drive the roller assembly to be matched with the clamping block; a sample bottle clamp; the rack is used for positioning and installing the sample bottle clamp, the driving assembly and the relative position of the filter assembly, and the rack is provided with a positioning station for positioning and placing the filter assembly.
2. The automated bacterial harvesting device of claim 1, wherein: the clamping jaw is a manipulator which is arranged at a corresponding position on the frame and can clamp a closed pipeline.
3. The automated bacterial harvesting device of claim 1, wherein: the machine frame comprises a first machine frame sliding on the machine frame, a second machine frame which is fixedly arranged relatively, a positioning station for positioning and placing the filter assembly is arranged on the first machine frame, and a driving assembly and a clamping jaw are arranged on the second machine frame.
4. An automated bacterial harvesting device according to claim 1 or 3, wherein: the positioning fixture for positioning and clamping the bacteria collecting frame of the filter assembly is arranged on the positioning station for positioning and placing the filter assembly; the bacteria collecting frame is provided with a through hole matched with the clamping block by the roller assembly at the peristaltic pump assembly.
5. An automated bacterial harvesting device according to claim 3, wherein: the first rack is provided with a third driving structure for driving the first rack to slide.
6. The automated bacterial harvesting device of claim 4, wherein: the positioning clamp is provided with a fourth driving structure for driving the positioning clamp to open and clamp.
7. The automated bacterial harvesting device of claim 1, wherein: the sample bottle clamp is also included; the sample bottle clamp is arranged above the bacteria collecting frame; the sample bottle clamp can rotate up and down by 180 degrees; the two needles are arranged, and each filter cup body is communicated with each needle and a first pipeline between the two needles; the sample bottle clamp is provided with two groups.
8. The automated bacterial harvesting device of claim 7, wherein: the filter device is characterized by further comprising a pipeline, one end of the pipeline is connected with a first pipeline on each filter cup body, each needle is provided with a needle pipeline, and the other end of the pipeline is connected and communicated with each needle pipeline.
9. The automated bacterial harvesting device of claim 8, wherein: a first conversion head is arranged between the parallel pipeline and the needle head pipeline, and a second conversion head is arranged between the pipeline and the first pipeline.
10. The automated bacterial harvesting device of claim 1, wherein: the clamping block is provided with a concave cambered surface matched with the roller assembly.
11. The automated bacterial harvesting device of claim 1, wherein: the bottom of the fungus collecting frame is provided with a liquid discharge groove; the clamping jaw is provided with a fusing function.
12. The automated bacterial harvesting device of claim 1, wherein: and each filter cup body is provided with a first air filter.
13. The automated bacterial harvesting device of claim 7, wherein: and each needle is provided with a second air filter.
14. The automated bacterial harvesting device of claim 1, wherein: the device also comprises a limiting hoop arranged on the bacteria collecting frame for positioning the filtering cup body; the bacteria collecting frame is provided with a protective cover plate for installing and positioning the filter cup group, and the protective cover plate is provided with a fixing structure for fixing the protective cover plate.
CN202320352533.0U 2023-02-27 2023-02-27 Automatic fungus collecting device Active CN219441261U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202320352533.0U CN219441261U (en) 2023-02-27 2023-02-27 Automatic fungus collecting device

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202320352533.0U CN219441261U (en) 2023-02-27 2023-02-27 Automatic fungus collecting device

Publications (1)

Publication Number Publication Date
CN219441261U true CN219441261U (en) 2023-08-01

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
CN202320352533.0U Active CN219441261U (en) 2023-02-27 2023-02-27 Automatic fungus collecting device

Country Status (1)

Country Link
CN (1) CN219441261U (en)

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