CN219567922U

CN219567922U - Fungus collecting mechanism

Info

Publication number: CN219567922U
Application number: CN202320373158.8U
Authority: CN
Inventors: 刘兴海; 高凯斐; 徐磊; 葛焕飞; 沈志林
Original assignee: Zhejiang Tailin Life Science Co ltd
Current assignee: Zhejiang Tailin Life Science Co ltd
Priority date: 2023-02-27
Filing date: 2023-02-27
Publication date: 2023-08-22
Anticipated expiration: 2033-02-27

Abstract

The utility model discloses a bacteria collecting mechanism which comprises a filtering component, a rack and a peristaltic pump component. The utility model sets up the filter assembly and places the frame of the filter assembly, the first pipeline between needle head and filter cup forms the first pipeline group, the frame mounts and positions the filter cup of the filter assembly; and a peristaltic pump assembly is arranged to cooperate with the first tubing set of the filter assembly. The roller assembly of the peristaltic pump is matched with the clamping blocks to pump the sample liquid of the sample bottle into each filter cup body of the filter cup group, so that the automation of the installation and the filtration of the filter cup bodies is realized; finally, the sealing and the culture of the filter cup body can be completed through the clamping jaw. The peristaltic pump assembly can enable the bacteria collecting mechanism to be used independently. The use of the bacteria collecting mechanism is not limited by the air source.

Description

Fungus collecting mechanism

Technical Field

The utility model relates to a fungus collecting mechanism.

Background

Sterility testing is a test that checks whether a sample contains viable microorganisms that is not an alternative to a sterile product. According to the relevant legal requirements, the sterility test requires the filtration of the test sample or its solution or eluate in several parallel membrane filters which are closed and the cultivation of the microorganisms on the filter membranes. The existing sterile inspection adopts a peristaltic pump for driving, a hose is required to be installed and complex operation is required to be carried out for many times, the whole process is required to be completed through personnel operation, the labor intensity is high, the operation reproducibility is poor, and the control and the management of the inspection process are not facilitated.

The existing fungus collecting mechanism needs an air source to achieve liquid taking, and the fungus collecting mechanism cannot be separated from the air source to work independently, so that the application range of the fungus collecting mechanism is limited, and great inconvenience is caused to use.

Disclosure of Invention

The utility model aims to solve the problems in the prior art in use, and provides the bacteria collecting mechanism which can realize the process automation of sterile inspection, liberate operators, break through the filtration efficiency limit of the traditional mode, improve the working efficiency, and can be independently operated without an air source.

The technical scheme for solving the existing problems is as follows: a bacteria collection mechanism comprising: filter component, frame, peristaltic pump subassembly.

The filter assembly comprises a filter cup group and a needle head, wherein the filter cup group comprises at least one filter cup body, the filter cup group is provided with a first air filter, the needle head is provided with a second air filter, a first pipeline is communicated between the needle head and each filter cup body, and the first pipeline between the needle head and the filter cup body forms a first pipeline group.

The filtering component also comprises a plurality of clamping claws which can clamp the closed pipeline. The clamping jaw is provided with a fusing function.

And the rack is used for placing and installing the filter assembly.

The peristaltic pump assembly is used for pumping sample liquid into the filter cup body, is arranged on the frame and comprises a roller assembly and a clamping block which is arranged on the frame and is matched with the roller assembly to clamp the first pipeline group; the first pipeline group is at least positioned at the position matched with the roller assembly and is a hose.

As a further improvement, the roller assemblies and/or the clamping blocks are/is arranged on the frame.

As a further improvement, two needles are arranged, and each filter cup body is communicated with each needle head and a first pipeline between the two needles; the sample bottle clamp is provided with two groups.

As a further improvement, a sample bottle holder is also included. The sample bottle clamp is arranged above the frame. The sample bottle clamp can rotate up and down by 180 degrees.

As a further improvement, the filter device also comprises a pipeline, wherein one end of the pipeline is connected with a first pipeline on each filter cup body, each needle is provided with a needle pipeline, and the other end of the pipeline is connected and communicated with each needle pipeline.

As a further improvement, a first conversion head is arranged between the parallel pipeline and the needle head pipeline, and a second conversion head is arranged between the pipeline and the first pipeline.

As a further improvement, the clamping block is provided with a concave cambered surface matched with the roller assembly.

As a further improvement, the bottom of the frame is provided with a liquid discharge groove.

As a further improvement, each filter cup is provided with a first air filter.

As a further improvement, a second air filter is arranged on each needle.

As a further improvement, the filter cup also comprises a limiting hoop arranged on the frame for positioning the filter cup body; the machine frame is provided with a protective cover plate for installing and positioning the filter cup group, and the protective cover plate is provided with a fixing structure for fixing the protective cover plate.

Compared with the prior art, the utility model is provided with the filter assembly and the frame for placing the filter assembly, the first pipeline between the needle head and the filter cup body forms a first pipeline group, the frame is used for installing and positioning the filter cup body of the filter assembly, and the peristaltic pump assembly matched with the first pipeline group of the filter assembly is arranged. The roller assembly of the peristaltic pump is matched with the clamping blocks to pump the sample liquid of the sample bottle into each filter cup body of the filter cup group, and the automatic filtration device has the beneficial effects that the automation of the filtration of the filter cup bodies is realized; finally, the sealing and the culture of the filter cup body can be completed through the clamping jaw. The peristaltic pump assembly can enable the bacteria collecting mechanism to be used independently. The use of the bacteria collecting mechanism is not limited by the air source.

Drawings

Fig. 1 is a schematic structural view of the present utility model.

Fig. 2 is a schematic view of the structure of the filter of the present utility model.

Fig. 3 is a schematic view of a frame of the present utility model.

Fig. 4 is a schematic structural view of the clamp block and roller assembly in cooperation with the first pipe group.

Fig. 5 is a schematic diagram of a rack plumbing connection of the present utility model.

Fig. 6 is an enlarged schematic view at a of fig. 5.

Detailed Description

Referring to fig. 1-6, the bacteria collecting mechanism of the present embodiment includes: a filter assembly, a frame 1 and a peristaltic pump assembly 2.

The filter assembly comprises a filter cup group and a needle head 4, wherein the filter cup group comprises at least one filter cup body 3, the filter cup group is provided with a first air filter 31, the needle head 4 is provided with a second air filter 42, a first pipeline 5 is communicated between the needle head 4 and each filter cup body 3, and the first pipeline 5 between the needle head 4 and the filter cup body 3 forms a first pipeline group; the number of the filter cups 3 is usually 1-4, in this embodiment, three filter cups are taken as an example, and the specific number of the filter cups is determined according to actual needs.

The filter assembly further comprises a plurality of clamping jaws 12 which clamp the closed pipeline. The jaws 12 may be provided with a fusing function. During pumping, the corresponding lines may be closed by the jaws 12, as desired. The jaws 12 may be of prior art construction, the specific number of which may be set according to the filtration requirements. The clamping jaw 12 can be simply a spring clamp, or can be realized by a mechanical arm, such as a finger cylinder.

The frame 1 is used for placing and installing the filter assembly, and a shell can be arranged on the frame. For ease of use, sample bottle holder 11 may also be included; the sample bottle clamp 11 is arranged above the frame 1.

Peristaltic pump subassembly 2 is used for pumping into sample liquid in the filtration cup 3, peristaltic pump subassembly 2 set up in the frame corresponding place. The peristaltic pump assembly 2 comprises a roller assembly 22 and a clamping block 21 which is matched with the roller assembly 22 and clamps the first pipeline group. The roller assembly 22 includes upper and lower covers, and a plurality of rollers which are clamped between the upper and lower covers and can be matched with the clamping blocks 21. The rollers are uniformly distributed on the circumferences of the upper cover body and the lower cover body. The upper cover body or the lower cover body is provided with a driving motor for driving the upper cover body and the lower cover body to rotate, and the rotation axis is the axis of the circumference; the drive motor may be internal or external. The rotating upper cover body and the rotating lower cover body drive the rollers uniformly distributed on the circumference of the ring to rotate so as to periodically drive the first pipeline group between the pressing and clamping blocks 21, and the power source for pumping the liquid into the filter cup body is realized through the cooperation of the roller assemblies 22 and the first pipeline group. The first tubing set and peristaltic pump assembly 2 may be constructed in accordance with known techniques. The first pipeline group is at least positioned at the position matched with the roller assembly and is a hose.

Preferably, the roller assembly 22 and/or the clamping block 21 are/is mounted on the frame. Of course, the clamping block 21 and/or the rotating wheel assembly 22 can also be mounted on an external bracket, and the clamping block 21 and the rotating wheel assembly 22 can be matched with the hose portion of the first pipeline group for use through the external bracket, and even an external power assembly can be further arranged to drive the extrusion clamping block 21 and the rotating wheel assembly 22 to clamp the hose portion and match with the hose portion.

For continuous operation, the needles 4 are provided with two, and each filter cup 3 is communicated with a first pipeline 5 between each needle 4 and the filter cup; the sample bottle clamp 11 is provided with two groups.

The sample bottle clamp 11 can rotate up and down by 180 degrees, and can rotate manually or through motor power. The sample bottle clamp 11 can be installed on the frame 1, and can also be installed on the external bracket 1 to keep a matched relationship with the bracket 1 so as to facilitate sampling.

In order to simplify the connection, the filter cup further comprises a parallel pipeline 6, one end of the parallel pipeline 6 is connected with the first pipeline 5 on each filter cup 3, each needle 4 is provided with a needle pipeline 41, and the other end of the parallel pipeline 6 is connected with and communicated with each needle pipeline 41.

The first conversion head 61 is arranged between the parallel pipeline 6 and the needle pipeline 41, the first conversion head 61 can be a tee joint, the second conversion head 62 is arranged between the pipeline 6 and the first pipeline 5, and the second conversion head 62 can be a four-way joint.

The clamp block 21 is provided with a concave cambered surface 23 matched with the roller assembly 22. The bottom of the frame 1 is provided with a liquid discharge groove.

In this embodiment, each filter bowl 3 is provided with a first air filter 31. A second air filter 42 is provided on each needle 4.

The filter cup body 3 is positioned on the frame 1, and the filter cup body also comprises a limiting hoop 14 arranged on the frame 1. The limiting hoops 14 can be convenient for the filter cup body 3 to be quickly clamped into the frame 1, and the frame 1 can be provided with a protective cover plate 15 for installing and positioning the filter cup group. The protective cover plate 15 is provided with a fixing structure 13 for fixing the protective cover plate 15, the fixing structure 13 can be an electromagnet arranged on the outer side of the frame 1, and a bent arm clamping column for fastening the protective cover plate 15 is arranged on the electromagnet.

In operation, the filter cup 3 of the filter assembly is fixed on the frame 1, and the filter cup 3 can be mounted on the frame through the base 32. The first tubing set is pre-installed between the clamp block 21 and the roller assembly 22 of the peristaltic pump assembly. If the entire peristaltic pump assembly is mounted directly on the frame 1, the hose portion of the first tubing set is pre-positioned between the clamp block 21 and the roller assembly 22. If the clamping block 21 and/or the roller assembly 22 of the peristaltic pump assembly are/is mounted on an external support, the clamping block 21 and the roller assembly 22 are positioned at corresponding positions, the hose portion of the first tube set needs to be adjusted between the clamping block 21 and the roller assembly 22, and then the clamping block 21 is driven by a manual or pneumatic cylinder, hydraulic cylinder or other prior art structure to cooperate with the roller assembly 22 to clamp the hose portion of the first tube set.

One sample bottle may be mounted in one of the sample bottle holders 11 as desired, or preferably two sample bottles are mounted in each of the two sets of sample bottle holders 11. Any needle can be inserted as a first needle into the rubber plug of a sample vial, typically placed upside down on the sample vial holder 11, either manually or by an external manipulator. The jaw 12 is first used to close the needle line of another needle, which acts as a second needle. The peristaltic pump assembly 2 is started, the peristaltic pump assembly 2 starts to work, and the sample liquid of the sample bottle connected with the first needle is transferred and pumped into the three filtering cups 3, and filtering is started. Before the liquid in the sample bottle inserted by the first needle is pumped out, the second needle is inserted into the rubber plug of another sample bottle, and the sample liquid in the sample bottle inserted by the second needle is transferred and pumped into the three filter cups 3. The clamping jaw 12 on the needle pipe of the second needle is opened, the needle pipe of the first needle is closed by the clamping jaw 12, and the continuous pumping of the sample liquid into the filter cup body 3 is realized by analogy. The filtered liquid is discharged through the drain pipe of the filter bowl 3, and microorganisms are trapped on the filter membrane.

In order to facilitate the insertion of the needle, the sample bottle with the upward rubber plug can be placed in the sample bottle clamp 11 in advance, and after the needle is inserted, the sample bottle clamp 11 is rotated again to invert the sample bottle, so that the rubber plug end is downward, and the sample liquid can be conveniently transferred into the filter cup body 3.

The above operation is repeated to complete the filtration of all samples, then the lower drain pipe of each filter cup 3 is closed by the clamping jaw 12, meanwhile, two needle pipelines are closed by the clamping jaw 12, the sample liquid bottle is replaced by a bottle filled with culture medium, the first needle is inserted into a culture medium bottle, the needle pipeline of the first needle is opened, the first pipeline of one filter cup 3 is reserved to be opened, the first pipelines of the other filter cups 3 are closed by the clamping jaw 12, and the culture medium is transferred into one filter cup 3 with the first pipeline unblocked by the peristaltic pump assembly 2. The first and second needle operations in transferring the culture medium can refer to the sample liquid transferring process. Repeating the above operation, and sequentially filling the culture medium into the other residual filter cup bodies 3 until the filling of the culture medium in all the filter cup bodies 3 is completed. The first line and the air line of the three filter cups 3 are then closed by the clamping jaws 12, as shown in fig. 2. The three filter cups 3 form a fully-closed special culture container, and can be transferred to different temperatures for culture to determine whether the sample contains microorganisms.

If a needle is used, a needle is used to pump the liquid and medium into the filter bowl 3 in accordance with normal operation.

Claims

1. The utility model provides a fungus mechanism gathers which characterized in that: the filter assembly comprises a filter cup group and a needle head, wherein the filter cup group comprises at least one filter cup body, the filter cup group is provided with a first air filter, the needle head is provided with a second air filter, a first pipeline is communicated between the needle head and each filter cup body, and the first pipeline between the needle head and the filter cup body forms a first pipeline group; the filtering component also comprises a plurality of clamping claws which can clamp the closed pipeline; the rack is used for placing and installing the filter assembly; the peristaltic pump assembly is used for being matched with the first pipeline group and pumping sample liquid into the filter cup body, the peristaltic pump assembly is arranged at the corresponding position of the frame, and the peristaltic pump assembly comprises a roller assembly and a clamping block which is matched with the roller assembly and clamps the first pipeline group; the first pipeline group is at least positioned at the position matched with the roller assembly and is a hose.

2. The bacteria collection mechanism of claim 1, wherein: the roller assemblies or/and the clamping blocks are arranged on the frame.

3. The bacteria collection mechanism of claim 1, wherein: the sample bottle clamp is also included; the sample bottle clamp is arranged above the frame; the sample bottle clamp can rotate up and down by 180 degrees; the two needles are arranged, and each filter cup body is communicated with each needle and a first pipeline between the two needles; the sample bottle clamp is provided with two groups.

4. A bacteria harvesting mechanism as claimed in claim 3, wherein: the filter device is characterized by further comprising a pipeline, one end of the pipeline is connected with a first pipeline on each filter cup body, each needle is provided with a needle pipeline, and the other end of the pipeline is connected and communicated with each needle pipeline.

5. The bacteria collection mechanism of claim 4, wherein: a first conversion head is arranged between the parallel pipeline and the needle head pipeline, and a second conversion head is arranged between the pipeline and the first pipeline.

6. The bacteria collection mechanism of claim 1, wherein: the clamping block is provided with a concave cambered surface matched with the roller assembly.

7. The bacteria collection mechanism of claim 1, wherein: the bottom of the frame is provided with a liquid discharge groove; the clamping jaw is provided with a fusing function.

8. The bacteria collection mechanism of claim 1, wherein: and each filter cup body is provided with a first air filter.

9. The bacteria collection mechanism of claim 1, wherein: and each needle is provided with a second air filter.

10. The bacteria collection mechanism of claim 1, wherein: the filter cup body is positioned on the frame; the machine frame is provided with a protective cover plate for installing and positioning the filter cup group, and the protective cover plate is provided with a fixing structure for fixing the protective cover plate.