JP4013202B2 - Sampling tube cleaning method in blood sample sampling mechanism - Google Patents

Description

  The present invention relates to a method of sampling a sample such as blood collected by a sample container such as a blood collection tube and washing a sampling tube in a blood test apparatus or the like, and in particular, a sealed sample container and an open sample container. The present invention relates to a sampling tube cleaning method that can be performed appropriately and smoothly using a common sample sampling mechanism.

Today, an automatic blood cell counter is known as a typical example of a blood test apparatus. For example, as shown in FIG. 10, the automatic blood cell counter includes a blood quantification / dilution processor A, a blood cell detector B, and a counter processor C. In the automatic blood cell counter configured as described above, blood containing a certain amount of anticoagulant from the blood collection tube A1 is appropriately sampled into the dilution containers A3 and A4 via the sampling tube A2. Next, the blood sampled in the dilution containers A3 and A4 is transferred to the reaction chamber and the counting chamber together with an appropriate amount of diluent and hemolytic agent A5, and in the blood cell detector B, the red blood cell / platelet B1, white blood cell B2, and hemoglobin concentration B3 is detected. In this way, the measurement signals detected by the blood cell detector B are respectively subjected to arithmetic processing C1, C2, C3 by the counting processor C, and the measurement results are output and displayed by the display means C4. Yes.

  However, in the conventional blood cell counter configured as described above, for example, when blood in a blood collection tube is subjected to suction sampling into a dilution detector through a sampling tube for suction, sample blood adheres to the outer periphery of the sampling tube. If this occurs, this blood is mixed into the blood to be diluted, and the dilution factor will fluctuate. Therefore, in such a case, there is a problem that a significant measurement error occurs in the measurement result of the blood cell count.

  From this point of view, the present applicant can remove the blood adhering to the outer periphery of the sampling tube (suction tube) without using a dedicated cleaning solution, and can accurately remove blood cells. Was developed and a patent was obtained (see Patent Document 1). That is, the blood cell counter described in Patent Document 1 is a blood cell counter including a suction tube that sucks sample blood and discharges it into a counting container that counts the number of blood cells in the blood. And a cleaning means for cleaning and removing the blood adhering to the outer periphery of the suction tube with a cleaning liquid after the sample blood has been sucked, and a cleaning drainage liquid for cleaning the counting container after blood cell counting for the suction tube It has a configuration in which liquid feeding means for feeding liquid to the washing means is provided as a cleaning liquid.

  Further, regarding a pipette device in an automatic analyzer configured to suck a required amount of liquid and discharge the liquid at another position, the pipette is bent even from inside a container with a rubber stopper. There has been proposed a pipette device having a simple configuration that can easily suck a liquid without damaging it and that does not require a separate liquid level detecting means (see Patent Document 2). That is, the pipette device described in Patent Document 2 includes a fixed outer cylinder and an inner cylinder that can be moved up and down and is housed in the fixed outer cylinder, and the inner cylinder and the fixed outer cylinder. The gap portion is configured to be supplied with cleaning water.

  Furthermore, as a sample collection device that can automatically collect blood from a blood collection tube in the past, when collecting a sample (blood) inside a sample container (collection tube) by piercing the nozzle into a rubber stopper, a collection nozzle is provided. A sampling device that prevents direct contact with the rubber stopper has been proposed (see Patent Document 3). That is, in the sample collecting device described in Patent Document 3, the nozzle portion is composed of an inner nozzle and an outer nozzle, and when puncturing the rubber stopper, the puncturing is performed with the inner nozzle stored in the outer nozzle. After the puncture, the sample is collected by lowering the tip of the inner nozzle from the tip opening of the outer nozzle, and after the sample is collected, the nozzle is cleaned using a cleaning tank. is there.

Japanese Patent Publication No. 7-119759 Japanese Utility Model Publication 4-898 Japanese Patent Laid-Open No. 7-83938

However, when conventionally definitive blood test, blood analysis, etc., as blood collection tube for performing the blood collection, the open and the closed type are used. Therefore, when sampling (sampling) samples from these blood collection tubes, different sampling mechanisms are used.
That is, when sampling from a sealed blood collection tube, it is necessary to use a sampling mechanism provided with a sampling tube whose tip is formed in a needle shape. On the other hand, when sampling from an open-type blood collection tube, the sampling mechanism including the sampling tube is dangerous, and its use is avoided. In addition, in order to perform sampling from a sealed blood collection tube with high accuracy, it is necessary to open the blood collection tube to the atmosphere in advance, and a mechanism for that is required. Therefore, it is difficult to make the sampling mechanism common to the open type and the sealed type blood collection tubes.

In general, in the case of a sealed blood collection tube, when an automated blood test apparatus or blood analysis apparatus is applied, it can be set so as to be integrated with a sampling mechanism for sampling. On the other hand, in the case of an open type blood collection tube, since manual operation is required for sampling, it is necessary to expose the sampling tube to the outside. For this reason, when the sampling tube is cleaned after each sampling, it is difficult to make the cleaning operation common because of the difference in operation of the sampling mechanism.

Accordingly, the present inventors have provided a method and apparatus that can perform appropriate and smooth sampling of the sealed-type blood collection tube and the open-type blood collection tube using a common sampling mechanism, and a sampling tube cleaning method. As a result of various examinations and trial manufactures, a piercing through which a sampling tube is inserted and a cleaning liquid drainage hole communicating therewith is provided and the sampling tube is inserted through the lower opening edge of the through hole so as to be movable up and down. The upper cleaning member with the needle connected and fixed is fixed, and the lower cleaning member provided with a through hole through which the piercing needle is inserted and a cleaning liquid water supply hole communicating therewith can be moved up and down below the upper cleaning member. to thereby use the sample sampling mechanism, such as blood obtained by construction arrangement, operation tie the one cleaning member and the sampling tube to the vertical movement By appropriately changing settings ring, and a cleaning for the sampling and the sampling tube of the sample by the sampling tube, I have found that it is possible to properly and smoothly achieved, respectively. It should be noted that the sample sampling mechanism in which the upper cleaning member is movable up and down and the lower cleaning member is fixed can similarly sample the sample with the sampling tube and clean the sampling tube.

That is, for the sealed sample container, (1) the lower cleaning member is set to a cleaning standby position surrounding the tip of the piercing needle , and the sampling tube is set to a standby position above the lower cleaning member. 2) Set a sealed sample container below the lower cleaning member, and (3) abut the sample container against the lower surface of the lower cleaning member and raise the lower cleaning member to contact the lower surface of the upper cleaning member. together to contact, pierce the piercing needle tip into the rubber stopper of the sample vessel, (4) a sampling tube is lowered, and inserted to a sampling position in the sample container, and samples of the sample, (5) after a predetermined sampling end with raising the sampling tube, comprising the steps of lowering the lower cleaning member to the cleaning standby position,
(6) The sampling tube, wherein the cleaning liquid drainage hole is raised to the cleaning position to open, washed by supplying washing liquid to the washing liquid drainage hole through the inside of the piercing needle from the cleaning water supply hole, (7) By collecting the sample container after completion of the predetermined cleaning and setting the sampling tube to be further raised to dispense the sampled sample, the dense sample can be sampled appropriately and smoothly, and sampling is performed. It was found that the tube could be cleaned smoothly.

For the open sample container, (1) the lower cleaning member is fixed to the cleaning standby position surrounding the tip of the piercing needle , and the sampling tube is lowered to the sampling position to be standby (2) Set an open sample container on the sampling tube, (3) insert the sampling tube to the sampling position in the sample container to sample the sample, and (4) after the predetermined sampling is completed, remove the sampling tube from the washing liquid drain. raising the hole to cleaning position for opening, the cleaning water supply hole from through the inside of the piercing needle was washed by supplying washing liquid to the washing liquid drain hole, (5) recovering said sample container after completion predetermined cleaning Then, by setting the sampling tube to be further raised and dispensing the sampled sample, in the same manner as described above, the sample is sampled from the open sample container. It is possible to perform the ring properly and smoothly, we have found that even the cleaning of the sampling tube can be smoothly performed.

  Therefore, an object of the present invention is to use a common sample sampling mechanism for a sealed sample container and an open sample container, respectively, to sample a sample by a sampling tube and thereafter clean the sampling tube. An object of the present invention is to provide a method of cleaning a sampling tube in a sample sampling mechanism for blood or the like that can be performed appropriately and smoothly.

In order to achieve the above object, a method for cleaning a sampling tube in a sample sampling mechanism for blood or the like according to claim 1 of the present invention is provided with a through hole through which the sampling tube is inserted and a cleaning liquid drain hole in communication therewith. And an upper cleaning member in which a piercing needle that allows a sampling tube to be moved up and down is connected to the lower opening edge of the through hole, and a through hole through which the piercing needle is inserted below the upper cleaning member. In a method for cleaning a sampling tube in a sample sampling mechanism such as blood, in which a lower cleaning member provided with a cleaning liquid water supply hole is configured and arranged so that one of them can be moved up and down relatively ,
(1) setting the lower cleaning member at a cleaning standby position surrounding the tip of the piercing needle, and waiting the sampling tube at a position above the lower cleaning member;
(2) setting a sealed sample container below the lower cleaning member;
(3) contact the sample container to the lower surface of the lower cleaning member those, Togedorisuru raises the lower cleaning member is brought into contact to the lower surface of the upper cleaning member, said piercing needle tip into the rubber stopper of the sample container Process,
(4) Lowering the sampling tube, inserting it to the sampling position in the sample container, and sampling the sample;
(5) with increasing the sampling tube after a predetermined sampling end, the steps of lowering the lower cleaning member to the cleaning standby position,
(6) sampling tube, wherein increasing the cleaning liquid drain holes to the cleaning position where the opening, a washing step of supplying a cleaning liquid to the cleaning liquid drainage hole from the washing liquid water supply hole via the inside of the piercing needle, in that it consists of Features.

According to a second aspect of the present invention, there is provided a method for cleaning a sampling tube in a sample sampling mechanism for blood or the like, wherein a through-hole through which the sampling tube is inserted and a cleaning liquid drain hole in communication therewith are provided and a lower opening edge of the through-hole and the upper cleaning member and communicating secure the piercing needle for inserting the sampling tube vertically movably in part, provided with washing liquid water hole communicating hole and to inserting said piercing needle at the lower of the upper cleaning member In the method of cleaning the sampling tube in the sample sampling mechanism such as blood, wherein the lower cleaning member is relatively arranged so that one of the members can be moved up and down ,
(1) fixing the lower cleaning member to a cleaning standby position surrounding the tip of the piercing needle , and lowering and waiting the sampling tube to the sampling position;
(2) a step of setting an open sample container in the sampling tube;
(3) Inserting the sampling tube to the sampling position in the sample container and sampling the sample;
(4) a sampling tube after a predetermined sampling end, the raising of the cleaning liquid drain holes to the cleaning position for opening, cleaning step of feeding cleaning liquid to the cleaning liquid drainage hole from the washing liquid water supply hole via the inside of the piercing needle and It is characterized by comprising.

According to a third aspect of the present invention, there is provided a sampling tube cleaning method in a blood sample sampling mechanism according to the present invention, wherein the upper cleaning member is fixedly arranged in the sample sampling mechanism such as blood in the sampling tube cleaning method, The lower cleaning member is configured and arranged to be vertically movable below the upper cleaning member .

According to a fourth aspect of the present invention, there is provided a method for cleaning a sampling tube in a sample sampling mechanism for blood or the like. the upper opening edge of the hole, the kicking set the O-ring surrounding holding the sampling tube, the lower cleaning member, the upper opening edge of the through hole, O-ring surrounding retaining said piercing needle characterized by comprising the structure in which a.

According to the method for cleaning a sampling tube in a sample sampling mechanism for blood or the like according to claim 1 of the present invention, a through hole through which the sampling tube is inserted and a cleaning liquid drain hole in communication with the through hole are provided, and a lower opening of the through hole is provided. An upper cleaning member having a piercing needle that allows the sampling tube to be freely moved up and down is fixed to the edge, a through hole through which the piercing needle is inserted, and a cleaning liquid water supply hole that communicates with the upper cleaning member. Using a sample sampling mechanism, such as blood, which is constructed so that one of the lower cleaning members can be moved up and down relatively , the lower cleaning member is attached to the tip of the piercing needle of the upper cleaning member. In the case of using a sealed sample container, the sampling tube is fixed to the surrounding cleaning standby position, and the sampling tube is lowered to the sampling position to stand by. From sampling, it is possible to smoothly achieve the cleaning of the sampling tube in a subsequent.

  According to the method for cleaning the sampling tube in the sample sampling mechanism according to claim 2 of the present invention, the lower cleaning member is mounted on the upper part using the sample sampling mechanism such as blood applied to the sealed sample container. The cleaning member is fixed at a cleaning standby position that surrounds the tip of the piercing needle, and the sampling tube is lowered to the sampling position to stand by, thereby sampling from the sample in the case of using an open sample container, and subsequent sampling. Tube cleaning can be achieved smoothly.

Furthermore, according to the sampling tube cleaning method in the sample sampling mechanism according to claim 3 of the present invention, as the sample sampling mechanism , the upper cleaning member is fixedly disposed, and the lower cleaning member is vertically movable. By doing so, similarly to the above , the sampling tube can be smoothly washed after the sample is sampled.

According to the sampling tube cleaning method in the sample sampling mechanism according to claim 4 of the present invention, sample sampling that can be commonly applied to the sealed sample container and the open sample container, respectively. The mechanism can be used to smoothly achieve sample sampling and subsequent cleaning of the sampling tube .

  Next, an embodiment of a sampling tube cleaning method in a sample sampling mechanism for blood or the like according to the present invention will be described in detail with reference to the accompanying drawings.

  1 (a) to 1 (g) show an example of blood sampling of a sampling tube in the case of using a sealed blood collection tube 20A together with an embodiment of a blood sampling mechanism for performing a method of cleaning a sampling tube according to the present invention. It is schematic structure explanatory drawing which shows the process from the start to the completion | finish of washing | cleaning.

  First, in FIG. 1, the blood sampling mechanism used in the present embodiment includes a fixed upper cleaning member 10 and a lower cleaning member 14 that can move up and down with respect to the upper cleaning member 10. The upper cleaning member 10 has a through hole 10a through which the sampling pipe 16 is inserted and a cleaning liquid drain hole 10b, and an O-ring is provided at the upper opening edge of the through hole 10a so as to surround and hold the sampling pipe 16. 11 is provided, and a piercing needle 12 is connected and fixed to the lower opening edge of the through hole 10a. The lower cleaning member 14 has a through hole 14a through which the piercing needle 12 is inserted and a cleaning liquid water supply hole 14b. The upper opening edge of the through hole 14a surrounds and holds the piercing needle 12. Is provided with an O-ring 15 (see FIG. 1A).

  Next, in the blood sampling mechanism having the above-described configuration, the blood sampling operation by the sampling tube 16 and the cleaning operation of the sampling tube 16 in the case where the sealed blood collection tube 20A sealed by the rubber stopper 21 is a target will be described. 3 will be described with reference to a flowchart showing the operation process (A).

In FIG. 1A, first, the lower cleaning member 14 is set to a cleaning standby position where the tip of the piercing needle 12 of the upper cleaning member 10 is surrounded by an O-ring 15. At this time, the sampling tube 16 stands by at a position above the upper cleaning member 10 (STEP-11).
In FIG. 1B, a sealed blood collection tube 20A is set below the lower cleaning member 14 (STEP-12).
In the (c) 1, a blood collection tube 20A to the lower surface of the lower cleaning member 14 abuts and raises the lower cleaning member 14 is brought into contact to the lower surface of the upper cleaning member 10, the piercing needle 12 The tip is pierced through the rubber stopper 21 of the blood collection tube 20A (STEP-13).
In FIG. 1 (d), the sampling tube 16 is lowered and inserted to the sampling position in the blood collection tube 20A to sample blood (STEP-14).
In FIG. 1 (e), the sampling tube 16 is raised after completion of predetermined sampling, and the lower cleaning member 14 is lowered to the cleaning standby position (STEP-15).
In FIG. 1 (f), the sampling tube 16 is raised to the cleaning position with respect to the upper cleaning member 10, and the upper cleaning member is passed from the cleaning liquid supply hole 14 b of the lower cleaning member 14 through the inside of the piercing needle 12. Cleaning is performed by supplying the cleaning liquid to the 10 cleaning liquid drain holes 10b under negative pressure conditions (STEP-16). In this case, the blood adhering to the outer periphery of the sampling tube 16 is scraped and collected inside the through hole 10a of the upper cleaning member 10 and the piercing needle 12 by the action of the O-ring 11 when rising. When the lower cleaning member 14 descends, the adhering blood is scraped up inside the through hole 14a of the lower cleaning member 14 by the action of the O-ring 15, and is smoothly drained and washed to the outside by the flow of the cleaning liquid. .
In FIG. 1 (g), the blood collection tube 20A is recovered after completion of the predetermined washing, the sampling tube 16 is further raised, and the sampled blood is dispensed (STEP-17).
In this way, the state shown in FIG. 1A is entered, and the standby state for the next sampling operation of the blood collection tube 20A is established.

  FIGS. 2A to 2E show the blood sampling mechanism for performing the sampling tube cleaning method according to the present invention and the end of cleaning from the start of blood sampling of the sampling tube in the case of using the open-type blood collection tube 20B. It is schematic structure explanatory drawing which shows the process until. The blood sampling mechanism used in the present embodiment can be implemented by applying the blood sampling mechanism used in the first embodiment described above (see FIG. 1A).

  Therefore, in the blood sampling mechanism having the above-described configuration, the blood sampling operation by the sampling tube 16 and the cleaning operation of the sampling tube 16 in the case where the open type blood collection tube 20B is the target, the operation process of FIG. This will be described with reference to the flowchart shown in FIG.

2A, first, the lower cleaning member 14 is fixed at a cleaning standby position in which the tip of the piercing needle 12 of the upper cleaning member 10 is surrounded by an O-ring 15. At this time, the sampling tube 16 is lowered to the sampling position and waits (STEP-21).
In FIG. 2B, an open-type blood collection tube 20B is set to the sampling tube 16 (STEP-22).
In FIG. 2C, the sampling tube 16 is inserted to the sampling position in the blood collection tube 20B, and the sample is sampled (STEP-23).
2D, the sampling tube 16 is raised to the cleaning position with respect to the upper cleaning member 10 after the end of the predetermined sampling, and from the cleaning liquid supply hole 14b of the lower cleaning member 14 through the inside of the piercing needle 12. Then, the cleaning liquid is supplied to the cleaning liquid drain hole 10b of the upper cleaning member 10 under a negative pressure condition to perform cleaning (STEP-24). In this case, the blood adhering to the outer periphery of the sampling tube 16 is scraped and collected inside the through hole 10a of the upper cleaning member 10 and the piercing needle 12 by the action of the O-ring 11, and smoothly flows due to the circulation of the cleaning liquid. The waste water is washed outside.
In FIG. 2E, the blood collection tube 20B is collected after completion of predetermined washing, and the sampled sample is dispensed by further raising the sampling tube 16 (STEP-25).
In this way, the state shown in FIG. 2A is entered, and the standby state for the next sampling operation of the blood collection tube 20B is established.

  Next, an embodiment of a sample sampling apparatus capable of sampling and washing a sample such as blood according to the present invention will be described in detail with reference to the accompanying drawings.

  FIG. 4 is a schematic configuration diagram showing an embodiment of a blood sampling apparatus according to the present invention. That is, the blood sampling apparatus according to the present embodiment includes an upper cleaning member 10 and a lower cleaning member 14 that can move up and down with respect to the upper cleaning member 10 as shown in FIG. The upper cleaning member 10 has a through hole 10a through which the sampling pipe 16 is inserted and a cleaning liquid drain hole 10b, and an O-ring is provided at the upper opening edge of the through hole 10a so as to surround and hold the sampling pipe 16. 11 is provided, and a piercing needle 12 is connected and fixed to the lower opening edge of the through hole 10a. The lower cleaning member 14 has a through hole 14a through which the piercing needle 12 is inserted and a cleaning liquid water supply hole 14b. The upper opening edge of the through hole 14a surrounds and holds the piercing needle 12. Is provided with an O-ring 15. The above configuration is the same as the blood sampling mechanism shown in FIGS. 1 and 2 used in the first and second embodiments.

  However, in the blood sampling apparatus of the present embodiment, as shown in FIG. 4, a support arm 30 is provided at one end of the upper cleaning member 10, and this support arm 30 is fixedly arranged at a predetermined position. It is pivotally supported at one end of a feed screw 32 which is coupled to 31 and rotates. A part of a holder support arm 42 that supports a holder 40 for storing and holding the sealed blood collection tube 20 </ b> A via a hinge 41 is screwed to the feed screw 32. That is, the holder 40 is configured to move up and down by the action of the feed screw 32. The holder 40 is provided with an insertion hole 40a for inserting the sampling tube 16 therethrough. Further, a support arm 34 is provided at one end of the lower cleaning member 14, and the support arm 34 is attached to a guide shaft 36 whose one end is coupled and fixed to the support arm 30 of the upper cleaning member 10. 42 is inserted. Then, a return spring 35 made of a compression coil spring is mounted between the support arm 30 and the support arm 34 of the guide shaft 36. Furthermore, a starter switch 46 that is operated when an open type blood collection tube 20B described later is used is provided on the holder lower support plate 44 that extends downward with respect to the holder support arm 42. Reference numeral 50 denotes an apparatus body case, and is set so that the start switch 46 is positioned inside the apparatus body when the holder 40 is in the lowered position.

  The blood sampling apparatus of the present embodiment configured as described above is configured to perform the blood sampling operation using the sampling tube 16 and the sampling tube when the sealed blood collection tube 20A is used as in the fourth embodiment shown in FIG. 16 cleaning operations can be performed appropriately and smoothly. In addition, as shown in FIG. 7, the blood sampling device of the present embodiment performs setting of the holder 40 and changes the operation of the sampling tube 16 to change the operation of the sampling tube 16, as shown in FIG. When using the open-type blood collection tube 20B, the blood sampling operation and the sampling tube 16 washing operation by the sampling tube 16 can be performed appropriately and smoothly.

  About the blood sampling operation by the sampling tube 16 and the washing operation of the sampling tube 16 in the case of targeting the sealed blood collection tube 20A using the blood sampling device having the above-described configuration, FIG. A description will be given with reference to a flowchart showing the operation process of FIG. 6 together with the schematic configuration shown in g).

5A, first, the lower cleaning member 14 is set to a cleaning standby position in which the tip of the piercing needle 12 of the upper cleaning member 10 is surrounded by the O-ring 15. At this time, the sampling tube 16 stands by at a position above the upper cleaning member 10. Then, the holder 40 is opened (STEP-31).
In FIG. 5B, after the sealed blood collection tube 20A is set in the holder 40, the holder 40 is closed (STEP-32).
In FIG. 5C, the drive motor 31 is rotationally driven to raise the holder 40 by the action of the feed screw 32, the blood collection tube 20A is brought into contact with the lower surface of the lower cleaning member 14, and the lower cleaning member 14 is further moved. While raising against the elastic force of the return spring 35 to bring it into contact with the lower surface of the upper cleaning member 10, the tip of the piercing needle 12 is pierced through the rubber stopper 21 of the blood collection tube 20A (STEP 33).
In FIG. 5 (d), the sampling tube 16 is lowered and inserted to the sampling position in the blood collection tube 20A to sample blood (STEP-34).
In FIG. 5 (e), the sampling tube 16 is raised after completion of predetermined sampling, the drive motor 31 is driven to rotate and the holder is lowered by the action of the feed screw 32, and the elastic force of the return spring 35 is used for lower cleaning. The member 14 is lowered to the cleaning standby position (STEP-35).
In FIG. 5F, the sampling tube 16 is raised to the cleaning position with respect to the upper cleaning member 10, and the upper cleaning member is passed from the cleaning liquid supply hole 14 b of the lower cleaning member 14 through the inside of the piercing needle 12. Cleaning is performed by supplying the cleaning liquid to the 10 cleaning liquid drain holes 10b under a negative pressure condition (STEP-36). In this case, the blood adhering to the outer periphery of the sampling tube 16 is scraped and collected inside the through hole 10a of the upper cleaning member 10 and the piercing needle 12 by the action of the O-ring 11 when rising. When the lower cleaning member 14 descends, the adhering blood is scraped up inside the through hole 14a of the lower cleaning member 14 by the action of the O-ring 15, and is smoothly drained and washed to the outside by the flow of the cleaning liquid. .
In FIG. 5 (g), the sampling tube 16 is further raised after the predetermined washing is finished to dispense the sampled blood, and the holder is further lowered by the action of the feed screw 32 by driving the drive motor 31 to rotate. Then, the holder 40 for storing and holding the blood collection tube 20A is opened (STEP-37).
In this way, the state shown in FIG. 5A is obtained, and a standby state for the sampling operation of the next blood collection tube 20A is established.

  FIG. 8A shows a blood sampling operation using the sampling tube 16 and a cleaning operation for the sampling tube 16 in the case where the blood sampling apparatus 20B having the above-described configuration is used for the open blood collection tube 20B. It demonstrates, referring also the flowchart figure which shows the operation | movement process of FIG. 9 with the schematic structure shown to (e).

In FIG. 8A, first, the lower cleaning member 14 is fixed at a cleaning standby position in which the tip of the piercing needle 12 of the upper cleaning member 10 is surrounded by an O-ring 15. At this time, the drive motor 31 is rotationally driven, the holder 40 is fixed to the lower portion of the lower cleaning member 14 by the action of the feed screw 32, and the sampling tube 16 is lowered to the sampling position and waits (STEP-41).
In FIG. 8B, an open-type blood collection tube 20B is set in the sampling tube 16, and the sampling tube 16 is inserted into the blood collection tube 20B (STEP-42).
In FIG. 8C, the sampling tube 16 is inserted to the sampling position in the blood collection tube 20B, and the start switch 46 provided on the lower support plate 44 of the holder 40 is operated to sample blood (STEP-43). .
In (d) of FIG. 8, after completion of the predetermined sampling, the sampling tube 16 is raised to the cleaning position with respect to the upper cleaning member 10, and from the cleaning liquid supply hole 14b of the lower cleaning member 14 through the inside of the piercing needle 12, Cleaning is performed by supplying the cleaning liquid to the cleaning liquid drain hole 10b of the upper cleaning member 10 under a negative pressure condition (STEP-44). In this case, the blood adhering to the outer periphery of the sampling tube 16 is scraped and collected inside the through hole 10a of the upper cleaning member 10 and the piercing needle 12 by the action of the O-ring 11, and smoothly flows due to the circulation of the cleaning liquid. The waste water is washed outside.
In FIG. 8 (e), after completion of the predetermined cleaning, the sampling tube 16 is further raised to dispense the sampled sample (STEP-45).
In this way, the state shown in FIG. 8A is entered, and the standby state for the next sampling operation of the blood collection tube 20B is established.

  The preferred embodiment of the present invention has been described above. However, the present invention is not limited to the sampling tube cleaning method or blood sampling apparatus in the blood sampling mechanism of the above-described embodiment. Even if the member can be moved up and down and the lower cleaning member is fixed, the sample can be sampled by the sampling tube and the sampling tube can be cleaned, and the method for cleaning the sampling tube for various samples other than blood can be used. Not only can it be effectively applied as a sampling device, but many other design changes can be made without departing from the spirit of the present invention.

It is one Example of the washing | cleaning method of the sampling tube in sample sampling mechanisms, such as blood, which concerns on this invention, Comprising: (a)-(g) is wash | cleaned from the start of sampling of the sampling tube in the case of using a sealed type blood collection tube It is a schematic structure explanatory view showing each process up to the end. It is another Example of the washing | cleaning method of the sampling pipe | tube in sample sampling mechanisms, such as blood according to this invention, Comprising: (a)-(e) is from the start of sampling of a sampling pipe | tube in the case of using an open type blood-collecting tube. It is schematic structure explanatory drawing which shows each process until completion | finish of washing | cleaning. FIG. 2 is an operation process of a sampling tube cleaning method in a sample sampling mechanism for blood or the like according to the present invention, in which (A) is a flowchart showing the operation process shown in FIG. 1, and (B) is an operation process shown in FIG. It is a flowchart figure. It is a schematic block diagram which shows one Example of sample sampling devices, such as blood, concerning the present invention. (A)-(g) is explanatory drawing which shows the process from the sampling start to the end in the case of sampling blood from a sealed blood collection tube using the sample sampling device such as blood shown in FIG. . It is a flowchart figure which shows the operation | movement process of the sampling method in sample sampling devices, such as blood, which concerns on this invention shown in FIG. FIG. 5 is a schematic configuration diagram showing a setting state of the sample sampling device when blood is sampled from an open blood collection tube by the sample sampling device such as blood according to the present invention shown in FIG. (A)-(e) is explanatory drawing which shows the process from the sampling start to the end in the case of sampling blood from an open type blood collection tube in the setting state of sample sampling devices, such as blood shown in FIG. . It is a flowchart figure which shows the operation | movement process of the sampling method in sample sampling devices, such as blood, concerning the present invention shown in FIG. 1 is a schematic block connection diagram showing a system configuration of an automatic blood cell counter.

Explanation of symbols

10 Upper cleaning member 10a Through hole 10b Cleaning liquid drain hole 11 O ring 12 Pierce needle 14 Lower cleaning member 14a Through hole 14b Cleaning liquid water supply hole 15 O ring 16 Sampling tube 20A Sealed blood collection tube 20B Open type blood collection tube 30 Support Arm 31 Drive motor 32 Feed screw 34 Support arm 35 Return spring 36 Guide shaft 40 Holder 40a Sampling tube insertion hole 41 Hinge 42 Holder support arm 44 Holder lower plate 46 Start switch 50 Device body case

Claims (4)

An upper cleaning member provided with a through hole through which the sampling pipe is inserted and a cleaning liquid drain hole communicating with the through hole, and a pierce needle through which the sampling pipe is inserted into the lower opening edge of the through hole so as to be movable up and down are connected and fixed. A lower cleaning member provided with a through-hole through which the piercing needle is inserted and a cleaning liquid water supply hole communicating with the lower piercing needle below the cleaning member is configured such that one of them can be moved up and down relatively . cleaning method Oite sampling tube definitive to sample sampling mechanism,
(1) setting the lower cleaning member at a cleaning standby position surrounding the tip of the piercing needle, and waiting the sampling tube at a position above the lower cleaning member;
(2) setting a sealed sample container below the lower cleaning member;
(3) contact the sample container to the lower surface of the lower cleaning member those, Togedorisuru raises the lower cleaning member is brought into contact to the lower surface of the upper cleaning member, said piercing needle tip into the rubber stopper of the sample container Process,
(4) Lowering the sampling tube, inserting it to the sampling position in the sample container, and sampling the sample;
(5) with increasing the sampling tube after a predetermined sampling end, the steps of lowering the lower cleaning member to the cleaning standby position,
(6) The sampling tube, wherein the cleaning liquid drainage hole is raised to the cleaning position to the opening, a washing step of supplying a cleaning liquid through the interior of the piercing needle from the cleaning water supply hole to the cleaning liquid drain hole,
A method for cleaning a sampling tube in a sample sampling mechanism for blood or the like, comprising: An upper cleaning member provided with a through hole through which the sampling pipe is inserted and a cleaning liquid drain hole communicating with the through hole, and a pierce needle through which the sampling pipe is inserted into the lower opening edge of the through hole so as to be movable up and down are connected and fixed. A lower cleaning member provided with a through-hole through which the piercing needle is inserted and a cleaning liquid water supply hole communicating with the lower piercing needle below the cleaning member is configured such that one of them can be moved up and down relatively . cleaning method Oite sampling tube definitive to sample sampling mechanism,
(1) fixing the lower cleaning member to a cleaning standby position surrounding the tip of the piercing needle , and lowering and waiting the sampling tube to the sampling position;
(2) a step of setting an open sample container in the sampling tube;
(3) Inserting the sampling tube to the sampling position in the sample container and sampling the sample;
(4) a sampling tube after a predetermined sampling end, the raising of the cleaning liquid drain holes to the cleaning position for opening, cleaning step of feeding cleaning liquid to the cleaning liquid drainage hole from the washing liquid water supply hole via the inside of the piercing needle and ,
A method for cleaning a sampling tube in a sample sampling mechanism for blood or the like, comprising: 3. The sample sampling mechanism for blood or the like in the sampling tube cleaning method according to claim 1 or 2, wherein the upper cleaning member is fixedly arranged, and the lower cleaning member can be moved up and down below the upper cleaning member. The method of cleaning a sampling tube according to claim 1 or 2, wherein the sampling tube is configured and arranged . The sample sampling mechanism according to any one of claims 1 to 3, wherein the sample sampling mechanism includes an O-ring that surrounds and holds the sampling tube at an upper opening edge of the through hole in the upper cleaning member. provided with, in the lower cleaning member, the upper opening edge of the hole, cleaning of the sampling tube according to any one of claims 1 to 3 consisting of configuration in which the O-ring that surrounds retaining said piercing needle Method.

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