CN110393135A - A kind of method of the sub- direct sowing and seedling of white flower pocket orchid species - Google Patents
A kind of method of the sub- direct sowing and seedling of white flower pocket orchid species Download PDFInfo
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- CN110393135A CN110393135A CN201910486589.3A CN201910486589A CN110393135A CN 110393135 A CN110393135 A CN 110393135A CN 201910486589 A CN201910486589 A CN 201910486589A CN 110393135 A CN110393135 A CN 110393135A
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- seedling
- white flower
- pocket orchid
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- seedbed
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/60—Flowers; Ornamental plants
- A01G22/63—Orchids
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/22—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
- A01G24/23—Wood, e.g. wood chips or sawdust
Abstract
The invention belongs to flower seedling raising technical field, especially a kind of method of the sub- direct sowing and seedling of white flower pocket orchid species, comprising the following steps: (1) processed bark and primary border topsoil are placed on cultivation tray in, seedbed is made after being irrigated with water, it is spare；(2) the mature fruit pod for selecting artificial pollination to obtain is seeded on seedbed after opening pericarp as seed, exploitation；(3) seedbed after planting is placed in ventilation, culture to seedling leaf is unearthed in wet culture environment, when seedling leaf reaches 2cm wide, can transplant.The present invention is planted in seedbed with white flower pocket orchid species, by precision management, the method for being achieved with a large amount of healthy and strong seedlings, it is simple and easy, and seedling easily survives, transplanting survival rate provides a kind of new approach up to 90% or more, for the large-scale breeding of white flower pocket orchid, efficiently solves the problems, such as current white flower pocket orchid plant resources child care and seedling resource shortage.
Description
Technical field
The invention belongs to flower seedling raising technical field, especially a kind of method of the sub- direct sowing and seedling of white flower pocket orchid species.
Background technique
White flower pocket blue (Paphiopedilum emersonii) belongs to orchid family (Orchidaceae) Paphiopedilum
(Paphiopedilum) herbaceos perennial was listed in pole danger endangered plants in 2004, is China endemic species, I grade of country
Plant is protected, there is the important ecological value and ornamental value.White flower pocket orchid only originates in South of Guizhou and In Northern Guangxi machine extremely
Under narrow Karst forest, Extant species are extremely rare, have been in the edge of extinction in imminent danger, vertical distribution is in height above sea level 430-
On 1000m steep cliff or disconnected rock wall.In order to alleviate the acquisition pressure of Wild Paphiopedilum platymiscium and meet people to cypripedium
Demand, the country be scholar the right artificial propagation of cypripedium is had conducted extensive research.
Currently, the aseptic seeding of part cypripedium and the artificial propagation techniques of tissue cultures have been succeeded, still
The problem that the generally existing culture medium of the artificial propagation techniques of white flower pocket orchid is complicated, condition of culture is harsh, incubation is cumbersome, manually
It breeds at high cost, greatly limits the development of white flower pocket orchid cultivation technique.Such as the text that number of patent application is 201610945622.0
A kind of tissue-culturing rapid propagation method for culturing seedlings of white flower pocket orchid disclosed in part, comprising the following steps: (1) white flower pocket orchid species is disinfected:
Specific steps are as follows: A, rinsed first with suds, use aseptic water washing later；B, again with 0.1% mercuric chloride impregnate 15 minutes, later
Use aseptic water washing；C, it is finally steeped 10 minutes with 75% alcohol, uses aseptic water washing later.(2) culture is sprouted in plantation: seed is sprouted
The culture medium that hair culture uses is living for 1/2MS+20ml/L coconut palm cream+20g/L sucrose+20g/L potato+0.1mg/LKT+0.3mg/L
Property charcoal+6-7g/L agar powder, PH5.7-5.5, dark culture 30-60 days, 25 ± 1 DEG C of cultivation temperature.(3) Protocorm: protocorm
The culture medium that stem culture uses is 1/2MS+0.2mg/LBA+20g/L banana+20g/L sucrose+0.3mg/L active carbon+6-7g/L
Agar powder, PH5.7-5.5, illumination cultivation 12hr/d, intensity of illumination 1500-1200LUX, 25 ± 1 DEG C of cultivation temperature.(4) it takes root
Culture: the culture medium that culture of rootage uses is 1/2MS+0.2mg/LNAA+0.1mg/LBA+20g/L banana+0.3mg/L active carbon
+ 6-7g/L agar powder, PH5.7-5.5, illumination cultivation 12hr/d, intensity of illumination 1500-1200LUX, 25 ± 1 DEG C of cultivation temperature.
(5) test tube transplantation of seedlings: after culture of rootage 60-90 days, finishing scouring seedling 7-15 days impregnates 15 basin clocks with 0.1% Bravo, after doing, moves
It plants, 10-28 DEG C of room temperature, air humidity 80%-90%, shade 60-70%.Although the method that the patent provides solves white flower pocket
The problem that blue percentage of seedgermination is low, seedling rooting rate is slow；But need for seed to be divided into 3 stages in different culture medium and reality
It tests under the conditions of room and is cultivated, and could be transplanted after cultivating successful test tube seedling and needing hardening；Incubation is cumbersome, toxigenic capacity
Height is not suitable for the extensive cultivation of white flower pocket orchid.
Summary of the invention
In order to solve the above technical problems existing in the prior art, researcher of the present invention sprouts white flower pocket orchid species
Hair rule and growth conditions are studied, and a kind of seedling-raising technique that white flower pocket orchid species is broadcast live is proposed, specifically logical
Cross following technical scheme realization:
A kind of method of the sub- direct sowing and seedling of white flower pocket orchid species, which is characterized in that including following procedure:
(1) processed bark and primary border topsoil are mixed and is placed in cultivation tray, seedling is made after being irrigated with water
Bed, it is spare；
(2) mellow fruit for selecting artificial pollination to obtain is seeded on seedbed after peeling off pericarp as seed；
(3) seedbed after planting is placed in ventilation, culture to seedling leaf is unearthed in wet culture environment, often after planting
It sprinkles profoundly water in week 2 times, when seedling leaf reaches 2cm wide, can transplant.
Preferably, the bark is the loose bark of horse hair.
Preferably, the processing method of the bark are as follows: the loose bark of horse hair is made in 2-4 centimetres of fritter, adds water submerged
It impregnates 3 days.
Preferably, the weight ratio of the step (1), processed bark and primary border topsoil is 3:1.
Preferably, the step (1), overall thickness >=8cm of processed bark and primary border topsoil in cultivation tray.
Preferably, the temperature of the culture environment is 18-24 DEG C, humidity is 95% or more, intensity of illumination 250-
500lux。
Preferably, the step (2), fruit are that the pericarp that 4-5 months fruits or field are collected after artificial pollination is white
The ripening fruits of green.
Preferably, the water is the tap water of mountain spring water or open standing after 3-7 days.The disease contained in the mountain spring water of flowing
Bacterium is few, avoids preferably infringement of the prevention germ to seedling；The standing of tap water can prevent the bleaching and sterilizing substance in tap water
(i.e. hypochlorous acid) interferes growth of seedling to the destruction of seedlings root.
The present invention studies the compost in cultivation: this researcher passes through to deodar, masson pine, Chinese pine, white
The pine barks such as Pi Song, Pinuns densata carry out screening study, the results show that with base made of Masson Pine Bark and primary border topsoil
The germination rate effect of matter, white flower pocket orchid species is good, and germination rate is 20.01% or so after sowing 90 days.Take processed horse hair
Pine bark and primary border topsoil change weight proportion, are placed in cultivation tray, with a thickness of 10cm, different seedbeds is made；
Then 130 days will be adopted back after artificial pollination with a batch, white flower pocket orchid species is seeded in seedbed similar in size, be placed in same ring
It is cultivated in border, observes the sprouting situation of seed, as shown in table 1:
Sprouting and growing state of 1 seed of table on the compost of different ratio
As can be known from Table 1, when the weight proportion of processed bark and primary border topsoil is 3:1, seed has very
Good germination rate, and the ball stem sprouted is able to maintain faster rate of differentiation；Researcher of the present invention also found Masson Pine Bark
It is made into the fritter of 2-4cm, germination rate also can be improved 8% or so, up to 23.4% or so.
The influence that the present invention is investigated kernel maturing and seed is sprouted: processed bark and primary border topsoil are taken
The earth weight proportion of 3:1 by weight ratio is placed in cultivation tray, and with a thickness of 10cm, seedbed is made；The difference adopted back is mature
The fruit pod of degree is seeded in seedbed, is placed in same environment and is cultivated, and the sprouting situation of seed is observed, as shown in table 2:
The sprouting situation of the seed of 2 differing maturity of table
As seen from the table, kernel maturing is higher, and after planting germination rate declines instead, this may is that it adapts to special habitats
A kind of strategy；120-150 days after artificial pollination, i.e. 4-5 months seeds have good germination rate.
The beneficial effects of the present invention are:
The present invention uses the compost of bark and primary border topsoil from a wealth of sources as white flower pocket orchid seed seedling-raising,
Greatly reduce the artificial breeding cost of white flower pocket orchid species；And it is not needed during seedling fostering according to its growth phase frequency
Its compost of numerous change and growing environment only need same culture substrate that can cultivate seedling under certain circumstances, into one
Step reduces its management cost.The present invention uses the culture substrate nursery containing soil, and the seedling cultivated is to transplanting environment tool
Being well adapted for property, not needing hardening can transplant, and transplant survival survival rate is up to 90% or more.
The present invention is planted in seedbed with white flower pocket orchid species, by precision management, so that it may obtain the side of a large amount of healthy and strong seedlings
Method, it is simple and easy, and easily survive, a kind of new approach is provided for the large-scale breeding of white flower pocket orchid, efficiently solves and works as
The problem of preceding the orchid plant resources child care of white flower pocket and seedling resource shortage.
Specific embodiment
It is limited below with reference to specific embodiment technical solution of the present invention is further, but claimed
Range is not only limited to made description.
Embodiment 1
A kind of method of the sub- direct sowing and seedling of white flower pocket orchid species, which is characterized in that including following procedure:
(1) processed Masson Pine Bark and primary border topsoil are mixed by the weight proportion of 3:1 and is placed on cultivation
In basin, with a thickness of 8cm, seedbed is made after being irrigated with mountain spring water, it is spare；
(2) 4 months fruits obtained after being manually pollinated is selected to be seeded on seedbed after peeling off pericarp as seed；
(3) seedbed after planting is placed in temperature is 18-24 DEG C, humidity is 95% or more, intensity of illumination 250-
It is cultivated in the culture environment of 500lux；It after planting irrigates weekly mountain spring water 2 times；There is protocorm, germination rate in 3 months or so in culture
20.3%；When seedling leaf reaches 2cm wide, be transplanted in the cultivation matrix as original sowing soil, according to routine techniques into
Row management, survival rate is up to 92.3%.
Further, the processing method of the bark are as follows: dry Masson Pine Bark is made in 2-4 centimetres of fritter, adds water submerged
It impregnates 3 days.
Embodiment 2
A kind of method of the sub- direct sowing and seedling of white flower pocket orchid species, which is characterized in that including following procedure:
(1) processed bark and primary border topsoil are placed in cultivation tray by the weight proportion of 3:1, with a thickness of
Seedbed is made in 15cm after being irrigated with the tap water for standing 3-5 days, spare；
(2) 5 months mellow fruits obtained are seeded in seedbed after peeling off pericarp as seed after selecting artificial pollination
On；
(3) seedbed after planting is placed in 18-24 DEG C, the training that humidity is 95% or more, intensity of illumination is 250-500lux
It supports and is cultivated in environment；Tap water 2 times of standing 3-5 days are after planting irrigated weekly；There is protocorm in 3 months or so in culture, sprouts
Rate is 16.15%, and 6-7 month seedling leaves of culture are unearthed；When seedling leaf reaches 2cm wide, it is transplanted to the cultivation with former sowing
It in matrix, is managed according to routine techniques, survival rate is up to 94.3%.
Further, the processing method of the bark are as follows: dry Masson Pine Bark is made in 2-4 centimetres of fritter, adds water submerged
It impregnates 3 days.
Embodiment 3
A kind of method of the sub- direct sowing and seedling of white flower pocket orchid species, which is characterized in that including following procedure:
(1) processed bark and primary border topsoil are placed in cultivation tray by the weight proportion of 3:1, with a thickness of
Seedbed is made in 20cm after being irrigated with the tap water for standing 5-7 days, spare；
(2) 125 days mellow fruits obtained are seeded in seedbed after peeling off pericarp as seed after selecting artificial pollination
On；
(3) seedbed after planting is placed in 18-24 DEG C, the training that humidity is 95% or more, intensity of illumination is 250-500lux
It supports and is cultivated in environment；Tap water 2 times of standing 3-5 days are after planting irrigated weekly；There is protocorm in 3 months or so in culture, sprouts
Rate is 23.41%, and 7-8 month seedling leaves of culture are unearthed；When seedling leaf reaches 2cm wide, be transplanted to former sowing cultivation
It trains in matrix, is managed according to routine techniques, survival rate is up to 93.7%.
Further, the processing method of the bark are as follows: dry Masson Pine Bark is made in 2-4 centimetres of fritter, adds water submerged
It impregnates 3 days.
Embodiment 4
A kind of method of the sub- direct sowing and seedling of white flower pocket orchid species, which is characterized in that including following procedure:
(1) processed bark and primary border topsoil are placed in cultivation tray by the weight proportion of 3:1, with a thickness of
Seedbed is made in 20cm after being irrigated with mountain spring water, spare；
(2) selecting the pericarp of field collection is the ripening fruits of white green as seed, is seeded in seedbed after peeling off pericarp
On；
(3) seedbed after planting is placed in 18-24 DEG C, the training that humidity is 95% or more, intensity of illumination is 250-500lux
It supports and is cultivated in environment；It after planting irrigates weekly mountain spring water 2 times；It cultivates and protocorm occurs within 3 months or so, germination rate 14.82%,
7-8 month seedling leaves are cultivated to be unearthed；When seedling leaf reaches 2cm wide, be transplanted to former sowing cultivation matrix in, according to
Routine techniques is managed, and survival rate is up to 93.7%.
Further, the processing method of the bark are as follows: dry Masson Pine Bark is made in 2-4 centimetres of fritter, adds water submerged
It impregnates 3 days.
Comparative example 1
Comparative example 1 the difference from embodiment 1 is that in cultivation tray processed bark and primary border topsoil thickness
It is 6-7cm lower than 8cm；Remaining seedling raising process is identical.After seed is seeded in seedbed, the germination rate of seed is lower than embodiment 1, is
7.6%, and the growth rate of protocorm is lower than embodiment 1；It may be the inadequate reason of moisturizing degree.
Comparative example 2
The difference of comparative example 2 and embodiment 4 is that intensity of illumination is 150-200lux lower than 250lux；Remaining nursery
Cheng Xiangtong.It after seed is seeded in seedbed, 4 months or so and just sprouts, and germination rate is lower than embodiment 4, be 9.8%.
Comparative example 3
The difference of comparative example 3 and embodiment 4 is that intensity of illumination is higher than 500lux, is 600-1000lux；Remaining nursery
Cheng Xiangtong.After seed is seeded in seedbed, seed proposes or so the last week sprouting, but germination rate is far below embodiment 4, is 6.4%.
It is important to point out that, above embodiments and test example are only limitted to do further technical solution of the present invention herein
Elaboration and understanding, should not be understood as it is further to technical solution of the present invention limited, what those skilled in the art made
The innovation and creation of non-protruding essential characteristics and marked improvement still fall within protection category of the invention.
Claims (8)
1. a kind of method of the sub- direct sowing and seedling of white flower pocket orchid species, which is characterized in that including following procedure:
(1) processed bark and primary border topsoil are mixed and is placed in cultivation tray, seedbed is made after being irrigated with water, it is standby
With；
(2) mellow fruit for selecting artificial pollination to obtain is seeded on seedbed after peeling off pericarp as seed；
(3) seedbed after planting is placed in ventilation, culture to seedling leaf is unearthed in wet culture environment, seedling leaf reaches 2cm
When wide, it can transplant.
2. the method for the sub- direct sowing and seedling of white flower pocket orchid species as described in claim 1, the bark is the loose bark of horse hair.
3. as claim 1 or 2 methods of the sub- direct sowing and seedling of white flower pocket orchid species stated, which is characterized in that the place of the bark
Reason method are as follows: Masson Pine Bark is made into the fritter of 2-4cm, water submerged is added to impregnate 3 days.
4. the method for the sub- direct sowing and seedling of white flower pocket orchid species as described in claim 1, which is characterized in that the step (1), processing
The weight ratio of the bark and primary border topsoil crossed is 3:1.
5. the method for the sub- direct sowing and seedling of white flower pocket orchid species as described in claim 1, which is characterized in that the step (1), cultivation
Overall thickness >=8cm of processed bark and primary border topsoil in basin.
6. the method for the sub- direct sowing and seedling of white flower pocket orchid species as described in claim 1, which is characterized in that the temperature of the culture environment
Degree is 18-24 DEG C, humidity is 95% or more, intensity of illumination 250-500lux.
7. the method for the sub- direct sowing and seedling of white flower pocket orchid species as described in claim 1, which is characterized in that the step (2), fruit
It is the pericarp that 4-5 months fruits or field are collected after artificial pollination is the ripening fruits of white green.
8. the method for the sub- direct sowing and seedling of white flower pocket orchid species as described in claim 1, which is characterized in that the water be mountain spring water or
Tap water of the opening standing after 3-7 days.
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| CN201910486589.3A CN110393135B (en) | 2019-06-05 | 2019-06-05 | Method for direct seeding and seedling raising of paphiopedilum albolanum seeds |
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| CN201910486589.3A CN110393135B (en) | 2019-06-05 | 2019-06-05 | Method for direct seeding and seedling raising of paphiopedilum albolanum seeds |
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2019
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