CN110313542A - A method of fermented feed is prepared using cotton byproduct - Google Patents
A method of fermented feed is prepared using cotton byproduct Download PDFInfo
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- CN110313542A CN110313542A CN201910721791.XA CN201910721791A CN110313542A CN 110313542 A CN110313542 A CN 110313542A CN 201910721791 A CN201910721791 A CN 201910721791A CN 110313542 A CN110313542 A CN 110313542A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
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Abstract
The present invention relates to a kind of methods for preparing fermented feed using cotton byproduct, it is uniformly mixed after cotton byproduct is crushed with cotton dregs powder, corn flour, salt, mineral powder and calcium monohydrogen phosphate, and water adjustment moisture content is added to be 65-75%, obtain basal fermentation raw material, then composite enzyme bacterium preparation is added, is encased in fermentor, is pressed and sealed after mixing, it ferments, fermented feed is obtained after 30d.The present invention is with cotton stalk and cotton seed hulls for main roughage, fermentation process is carried out to it using lactic acid bacteria and enzyme preparation, determine optimal fermenting additive or combination, to make the utilization rate of cotton byproduct effectively improve, the method of the present invention is easy to operate, it is easy to accomplish, it is at low cost, fermented fodder nutrient obtained is abundant, and palatability is good.
Description
Technical field
The present invention relates to a kind of preparation methods of fermented feed, especially a kind of to prepare fermented feed using cotton byproduct
Method belongs to fermented feed preparation technical field.
Background technique
Cotton in China yield occupies the first in the world, and gined cotton total output accounts for 27% or so of world's gined cotton total output.It is wherein new
The cotton planting gross area position of boundary is the first in the nation, and accounts about the half of national cotton total output, wherein the cotton of South Sinkiang
Cultivated area proportion is bigger, thus the cottons byproduct such as cottonseed, cotton seed hulls and cotton stalk resource is quite abundant, wherein cotton stalk
Yield tops the list.Most cotton byproduct especially cotton stalk using burning disposal or buried mode as fertilizer returning to the field, for example,
In Xinjiang, life in the countryside fuel about 25% is provided by cotton stalk, is used after more having 73% or so cotton stalk to be crushed
Make fertilizer and burn discarded, this not only can cause to seriously endanger to environment, the labour that can also aggravate the field farming of next year and
The expenditure of financial resources.
Containing cellulose, hemicellulose, lignin, organic matter and nitrogen, phosphorus, potassium and microelement etc. in cotton byproduct,
It is full of nutrition, it can be used as feed, but the mode directly herded after the picking of cotton field is extremely low to efficiency of feed utilization, and in cotton byproduct
The anti-nutritional factors content such as lignin, cellulose and hemicellulose and FG is higher, poor so as to cause palatability, digestibility is low
Deng, therefore effective rate of utilization is very low.
On the other hand, China's animal husbandry is faced with feed shortage problem now, and the insufficient and second-rate of feed resource will
As the principal element for restricting China's animal husbandry sustainable development.Therefore, rationally processing utilizes cotton byproduct, not only can solve feeding
The problem for expecting shortage, can also preserve the ecological environment.
Summary of the invention
It is an object of the invention to solve the deficiency of background technology part proposition, a kind of prepared using cotton byproduct is provided and is sent out
The method of ferment feed.This method is easy to operate, it is easy to accomplish, at low cost, fermented fodder nutrient obtained is abundant, and palatability is good.
Technical solution
A method of preparing fermented feed using cotton byproduct: by cotton byproduct crush after with cotton dregs powder, corn flour, food
Salt, mineral powder and calcium monohydrogen phosphate are uniformly mixed, and water adjustment moisture content is added to be 65-75%, obtain basal fermentation raw material, then plus
Enter composite enzyme bacterium preparation, be encased in fermentor after mixing, be pressed and sealed, ferment, fermentation is obtained after 30d and is raised
Material；
The cotton byproduct is cotton seed hulls and cotton stalk；
The composite enzyme bacterium preparation is by 1 × 109The lactobacillus preparation and enzyme preparation of CFU/ml forms.Lactic acid bacteria can provide battalion
Substance is supported, growth of animal is directly facilitated, improves alimentary canal micro-ecological environment, adjusts alimentary canal immune system, the addition of enzyme preparation
The content that DM can be improved, increases animal feed intake, reduces content of cellulose, improves feed nutritive value.
Further, the weight ratio of the cotton seed hulls and cotton stalk is 50:(9-10).
Further, the weight ratio of the cotton byproduct, cotton dregs powder, corn flour, salt, mineral powder, calcium monohydrogen phosphate are as follows: (55-
65):(22-26):(12-19):1:1:1。
Further, 5g composite enzyme bacterium preparation is added in every 1.5kg basal fermentation material.
Further, in the composite enzyme bacterium preparation, the weight ratio of lactobacillus preparation and enzyme preparation is 1:1.The lactic acid bacteria
Preparation is enterococcus faecium, and the enzyme preparation is cellulose degrading enzyme.
Further, the fermentation temperature is 25-30 DEG C.
Beneficial effects of the present invention: the present invention provides a kind of method for preparing fermented feed using cotton byproduct, this hairs
It is bright with cotton stalk and cotton seed hulls for main roughage, fermentation process is carried out to it using lactic acid bacteria and enzyme preparation, is determined optimal
Fermenting additive or combination, so that the utilization rate of cotton byproduct be made to effectively improve, the method for the present invention is easy to operate, is easy to real
Existing, at low cost, fermented fodder nutrient obtained is abundant, and palatability is good.
Specific embodiment
The present invention is further explained in the light of specific embodiments.
In following embodiments, the strain of lactobacillus preparation is enterococcus faecium, and from livestock technology, key lab is herbvore
Livestock nutrition research department；Enzyme preparation be cellulose degrading enzyme (vigor >=10000U/g of zytase, cellulase vigor >=
300U/g, vigor >=30U/g of mannase, vigor >=1500U/g of 1,4 beta-glucanase, neutral proteinase vigor >=
Vigor >=20U/g of 500U/g, glucose oxidase), it is purchased from Ningxia Sunson Industrial Group Co., Ltd..But not limited to this.
Embodiment 1
A method of preparing fermented feed using cotton byproduct: by 57.33 parts of cotton byproducts (cotton seed hulls and cotton stalk,
Mass ratio is 50:9.33) crush after with 24.67 parts of cotton dregs, 15 portions of corn flour, 1 portion of salt, 1 part of mineral powder, 1 part of calcium monohydrogen phosphate
It is uniformly mixed, adding water adjustment moisture content is 70%, basal fermentation raw material is obtained, then by being added in every 1.5kg basal fermentation material
The metered composite enzyme bacterium preparation of 5g composite enzyme bacterium preparation is (by 1 × 109The lactobacillus preparation and enzyme preparation of CFU/ml forms,
The weight ratio of lactobacillus preparation and enzyme preparation is 1:1, and the lactobacillus preparation is enterococcus faecium, and the enzyme preparation is cellulose drop
Solve enzyme), it is encased in fermentor, is pressed and sealed after mixing, 26 DEG C ferment, and fermented feed is obtained after 30d.
Embodiment 2
A method of fermented feed is prepared using cotton byproduct: by 60 parts of cotton byproducts (cotton seed hulls and cotton stalk, quality
Than being uniformly mixed after being crushed for 50:10) with 25 parts of cotton dregs, 12 portions of corn flour, 1 portion of salt, 1 part of mineral powder, 1 part of calcium monohydrogen phosphate,
Adding water adjustment moisture content is 70%, obtains basal fermentation raw material, then by addition 5g composite enzyme bacterium in every 1.5kg basal fermentation material
The metered composite enzyme bacterium preparation of preparation is (by 1 × 109The lactobacillus preparation and enzyme preparation of CFU/ml forms, lactobacillus preparation
Weight ratio with enzyme preparation is 1:1, and the lactobacillus preparation is enterococcus faecium, and the enzyme preparation is cellulose degrading enzyme), mixing
It is encased in fermentor, is pressed and sealed after uniformly, 28 DEG C ferment, and fermented feed is obtained after 30d.
Comparative example 1
Composite enzyme bacterium preparation is changed to deionized water, it is other same as Example 1.
Comparative example 2
Composite enzyme bacterium preparation is changed to 1 × 109The lactobacillus preparation (enterococcus faecium) of CFU/ml, it is other with 1 phase of embodiment
Together.
Comparative example 3
Composite enzyme bacterium preparation is changed to enzyme preparation (cellulose degrading enzyme), it is other same as Example 1.
Experiment test:
Fermented feed made from embodiment and comparative example 1-3 is carried out pH, dry matter (DM) content, crude protein (CP) to contain
Amount, neutral detergent fiber (NDF) and acid detergent fiber (ADF) content, ammoniacal nitrogen (NH3- N) it is content, organic acid content, free
The measurement of gossypol (FG) content etc., wherein crude protein (CP) content uses Kjeldahl nitrogen determination, neutral detergent fiber (NDF)
And acid detergent fiber (ADF) content is measured using the method for Van Soest, ammoniacal nitrogen (NH3- N) content use phenol-
Sodium hypochlorite colorimetric method for determining, organic acid content are measured using high performance liquid chromatograph (high performance liquid chromatograph (U3000)) method,
Free gossypol (FG) content is measured using spectrophotometer (ultra-violet and visible spectrophotometer (T6 new century)) method, DM, CP,
The test result of FG, NDF, ADF content is shown in Table 1, pH, NH3- N and organic acid content test result are shown in Table 1:
Table 1
As shown in Table 1, the fermented feed of embodiment 1, embodiment 2 is than the dry of comparative example 1, comparative example 2 and comparative example 3
Matter content is low, and crude protein content is high, this illustrates that the fermented feed ferment effect of the embodiment of the present invention is good；It can also be seen that implementing
The fermented feed of example 1 and embodiment 2 is than the free gossypol content, NDF content and ADF of comparative example 1, comparative example 2 and comparative example 3
Content is low, illustrates that free gossypol and fiber content in fermentation raw material can be effectively reduced in the embodiment of the present invention.
Table 2
Note: "-" expression is not detected.
As shown in Table 2, the pH of the fermented feed of Example 1 and Example 2 of the present invention is below comparative example 1,2 He of comparative example
3, and NH3The content of-N is below comparative example 1, embodiment 1, embodiment 2 the lactic acid content of fermented feed be higher than comparative example 1
With comparative example 3, acetic acid content is lower than comparative example 1, this illustrates that the embodiment of the present invention can efficiently use ammonia nitrogen and generate lactic acid.
Comprehensive Tables 1 and 2 can be seen that in the present invention in embodiment, and the addition of composite enzyme bacterium preparation makes in fermented feed
CP content and lactic acid content increase, and make ADF, NDF, FG, pH, NH3- N, acetic acid reduce, this illustrates fermented feed hair of the invention
Ferment effect is good.
Claims (7)
1. a kind of method for preparing fermented feed using cotton byproduct, which is characterized in that by cotton byproduct crush after with cotton dregs powder,
Corn flour, salt, mineral powder and calcium monohydrogen phosphate are uniformly mixed, and water adjustment moisture content is added to be 65-75%, obtain basal fermentation original
Then material is added composite enzyme bacterium preparation, is encased in fermentor, is pressed and sealed after mixing, ferment, after 30d to obtain the final product
To fermented feed；
The cotton byproduct is cotton seed hulls and cotton stalk；
The composite enzyme bacterium preparation is by 1 × 109The lactobacillus preparation and enzyme preparation of CFU/ml forms.
2. the method for preparing fermented feed using cotton byproduct as described in claim 1, which is characterized in that the cotton seed hulls and cotton
The weight ratio of tangerine bar is 50:(9-10).
3. the method for preparing fermented feed using cotton byproduct as described in claim 1, which is characterized in that the cotton byproduct,
The weight ratio of cotton dregs powder, corn flour, salt, mineral powder, calcium monohydrogen phosphate are as follows: (55-65): (22-26): (12-19): 1:1:1.
4. the method for preparing fermented feed using cotton byproduct as described in claim 1, which is characterized in that the every basis 1.5kg hair
5g composite enzyme bacterium preparation is added in ferment material.
5. the method for preparing fermented feed using cotton byproduct as described in claim 1, which is characterized in that the lactobacillus preparation
Weight ratio with enzyme preparation is 1:1.
6. the method for preparing fermented feed using cotton byproduct as described in claim 1, which is characterized in that the lactobacillus preparation
For enterococcus faecium, the enzyme preparation is cellulose degrading enzyme.
7. the method for preparing fermented feed using cotton byproduct as described in any one of claim 1 to 6, which is characterized in that described
Fermentation temperature is 25-30 DEG C.
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| CN201910721791.XA CN110313542A (en) | 2019-08-06 | 2019-08-06 | A method of fermented feed is prepared using cotton byproduct |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116218933A (en) * | 2023-04-20 | 2023-06-06 | 四川轻化工大学 | Two-stage mixed bacteria fermented cotton meal and preparation method and application thereof |
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| CN106417898A (en) * | 2016-09-21 | 2017-02-22 | 新疆农业大学 | Preparation method of microbiological preparation capable of increasing utilization rate of cotton straw and cottonseed hull feed, and application of microbiological preparation |
| CN108208347A (en) * | 2018-03-06 | 2018-06-29 | 新疆畜牧科学院饲料研究所 | A kind of enzyme bacterium mixture and method for reducing fiber content in cotton stalk |
| CN109832431A (en) * | 2017-11-24 | 2019-06-04 | 广西北流市红日紫瓷业有限公司 | A method of use cotton stalk to make fermented feed for raw material |
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Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1478410A (en) * | 2003-07-13 | 2004-03-03 | 熊仕玉 | Cotton straw detoxificated partical feed |
| CN105010840A (en) * | 2015-07-02 | 2015-11-04 | 新疆绿洲大洋生物科技有限公司 | Preparation method of cotton straw biological feed for stall-fed mutton sheep |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116218933A (en) * | 2023-04-20 | 2023-06-06 | 四川轻化工大学 | Two-stage mixed bacteria fermented cotton meal and preparation method and application thereof |
| CN116218933B (en) * | 2023-04-20 | 2024-03-08 | 四川轻化工大学 | Two-stage mixed bacteria fermented cotton meal and preparation method and application thereof |
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Application publication date: 20191011 |