CN107494928A - A kind of method of composite bacteria solid state fermentation mushroom bran production protein feed - Google Patents
A kind of method of composite bacteria solid state fermentation mushroom bran production protein feed Download PDFInfo
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- CN107494928A CN107494928A CN201710847086.5A CN201710847086A CN107494928A CN 107494928 A CN107494928 A CN 107494928A CN 201710847086 A CN201710847086 A CN 201710847086A CN 107494928 A CN107494928 A CN 107494928A
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- mushroom bran
- protein feed
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- mushroom
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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- Polymers & Plastics (AREA)
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- Food Science & Technology (AREA)
- Physiology (AREA)
- Animal Husbandry (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Health & Medical Sciences (AREA)
- Mycology (AREA)
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- Sustainable Development (AREA)
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Abstract
The invention belongs to feed production technology field, is specifically related to a kind of production method of mushroom bran solid state fermentation production animal feeding-stuff.It is characterized in that：Using the mushroom bran after string disassembler wet ground as matrix, composite bacteria and trophic factors are added, solid state fermentation is carried out after adding water, protein feed is made through drying；The present invention realizes the efficient utilization of mushroom bran, is advantageous to the protection of environment, while solves the problems, such as high nutritive value supply of forage deficiency.
Description
Technical field
The present invention relates to a kind of animal feeding-stuff production method using mushroom bran as main component, belongs to Feed Production Technology neck
Domain.
Background technology
Mushroom bran refers to harvest during edible fungus culturing remaining culture medium waste after product, and China is that Edible Fungi is big
State, national edible fungus bran total output in 2011 are 3.86 × 107 Ton.
With the expansion year by year of edible mushroom yield, edible fungus bran problem becomes increasingly conspicuous.Fuel quilt is made in mushroom bran part at present
Burning, mostly stack to rot, cause environmental pollution.
Relevant research shows, with the presence of substantial amounts of mycelia in mushroom bran, and in mycelia containing nutriments such as thick protein not
It is utilized, is good feed manufacturing raw material, there is higher value of exploiting and utilizing.
Meanwhile in the evolution of nowadays aquaculture, the dependence to feed industry is growing day by day, protein feed is not for should
Ask, much need to solve by import.
Based on above present situation, if it is possible to by mushroom bran resource rational utilization, while solving mushroom bran problem of environmental pollution,
The situation of feed resource shortage can be alleviated.
In numerous Land use systems, the mode that solid state fermentation production protein feed is carried out using mushroom bran is relatively conventional.
Solid state fermentation has the characteristics that：1）It is less demanding to fermentation substrate water content, reduce the dirt of bacterium and fungi
Dye；2）Improving throughput contributes to the raising of fermentation efficiency；3）Reaction unit is simple, and demand space is small；4）Energy consumption is low
Solid state fermentation can by microbial action by protein content in agriculture and forestry organic waste material it is low or be unfavorable for animal digestion absorb
Material is converted into the animal feed that nutritive value is high, utilization rate is high.
In published patent of invention, a kind of pleurotus eryngii edible fungi residue feed of patent of invention 201310259089.9, according to apricot Bao
Mushroom chaff 65-78 parts, corn flour 5-12 parts, dregs of beans 5-15 parts, the proportioning of alfalfa meal 6-17 parts are produced, and are not had
Pre-treatment is carried out to raw material, it is relatively low as feed, nutritive value without other additional nutritional ingredients；
A kind of preparation method of 201110443391.0 edible fungus bran feed of patent of invention, dry edible fungus bran is crushed
Auxiliary material and water are directly added after sieving, it is expanded after stirring, it is larger to carry out Feed Manufacturing energy consumption using this method；
Edible fungi residue feed of patent of invention 201310739861.7 and preparation method thereof, according to 80-90% mushroom bran powder；8-14%'s
Corn flour, wheat-middlings or fine rice bran；4-10% rapeseed dregs powder or cotton dregs powder；0.7-2% adjusting material；0.2-0.4% bacterium
Kind proportioning；The weight of wherein described adjusting material forms：0.15-0.3% ammonium sulfate；0.2-0.4% potassium chloride；
0.05-0.2% magnesium sulfate；0.3-1.2% wheat bran.It with the addition of nutriment and be vaccinated with probioticses, one in fermentation process
Determine to eliminate ANFs in degree, but crude fibre is not degraded well in raw material, does not improve its nutrition well
Value；
A kind of flammulina velutipes fungus chaff feeds of patent of invention 201310037881.X and its production method and application, the invention is directly to original
The enzyme available for lignocellulose degradation is with the addition of in material, production cost is higher, and other nutritional ingredients do not obtain in raw material
Make full use of；
To sum up, at present so that in the feed product that mushroom bran produces as raw material, crude fiber content is of a relatively high, crude protein content does not have
Improved to good, product has certain nutritive value, but digestibility is relatively low, and palatability is poor；In addition, it there are no to raw material
The technique for carrying out pre-treatment, directly inoculation carries out solid state fermentation strain and raw material is insufficient contact, can not fill during the fermentation
Divide the composition utilized in raw material；
Therefore want to produce the protein feed of high quality, it is also necessary to study these problems.
The content of the invention
The present invention is for overcome the deficiencies in the prior art, there is provided a kind of effectively to utilize edible fungus bran production protein feed
Production method, realize the recycling of mushroom bran, improve the level of comprehensive utilization of mushroom bran.
The invention provides a kind of method of solid state fermentation mushroom bran production protein feed, made with the mushroom bran after edible mushroom collection
For matrix, composite bacteria and trophic factors are added, solid state fermentation is carried out after adding a small amount of water, protein feed is made through drying.
Before inoculation composite bacteria and the various nutritional ingredient fermentations of addition, first by string disassembler to mushroom bran
Raw material carries out wet ground so that lignocellulosic softening, separation and Partial digestion in raw material, beneficial to entering for subsequent fermentation
OK.
Composite bacteria is inoculated with afterwards and adds trophic factors, solid state fermentation is carried out after adding a small amount of water, and egg is made through drying
White feed.
The material composition of solid state fermentation is：In every 80 ~ 90 parts of mushroom bran raw materials, 5 ~ 10 parts of water of addition, 5 ~ 10 parts of strains, 0.02
~ 0.03 part of bitter salt, 0.08 ~ 0.12 part of potassium dihydrogen phosphate, 0.8 ~ 2 part of glucose, 0.8 ~ 2 part of ammonium sulfate.
The strain of inoculation is made up of bacillus, aspergillus niger, aspergillus oryzae, Trichoderma viride and saccharomycete, and its ratio is 1 ~ 3:
1~3:1~3:1~3:1~3。
The wooden fibre that can degrade can be secreted after the bacillus that is inoculated with, aspergillus niger, aspergillus oryzae, Trichoderma Viride culture
The enzyme of element is tieed up, wherein bacillus is neutralized to separation screening in the liquid after neutral culture from alkaline lignin black liquor and drawn.
Fermentation method is：Bacillus is inoculated with into raw material first, under the conditions of 30 ~ 40 DEG C cultivate 2 ~ 4d, afterwards again to
Inoculated aspergillus niger, aspergillus oryzae, Trichoderma viride in raw material, cultivate 2 ~ 4d under the conditions of 30 ~ 40 DEG C, last inoculation yeast bacterium, 28 ~
2 ~ 3d is cultivated under the conditions of 32 DEG C.
The mushroom bran raw material crude protein content that the present invention uses is higher, adds a certain amount of trophic factors and inoculation thereto
After different strain is fermented, crude protein content can be further improved, improves product quality.
The composite bacteria solid state fermentation mushroom bran produces the specific method of protein feed：
（1）Mushroom bran raw material is broken into slurry, using Publication No. 101718049A patent of invention, a kind of entitled " stalk machine
String disassembler in tool method clean pulping process " carries out wet ground, which disclose a kind of purely mechanic stalk
Pulping process, using lignin between fiber at a certain temperature by the characteristic that glass transition is partially soft rubbery state, obtain fiber
To separation；
Concretely comprise the following steps：First by mushroom bran raw material moistening, swelling, size degradation, string disassembler is sent into afterwards, disassembler is using non-
Symmetrical dynamic, fixed millstone tear friction, mill is interior to produce 120-140 DEG C of high temperature, equivalent to digestion, beneficial to lignin in mushroom bran
Softening, degraded；Compared to general pulverization process, wet ground energy consumption is smaller, more cleans；
（2）Strain and trophic factors are added in mushroom bran by a certain percentage, is supplemented a certain amount of water, carries out fermented and cultured；
（3）The product fermented is dried using roller drier, product crushed using pulverizer after drying；Institute
Obtained product indices meet forage health standard.
Strain used in the present invention and its function：
（1）Saccharomycete：It contains the materials such as abundant protein, B family vitamin, amino acid, can promote growing for animal,
Shorten feeding period, improve meat, the resistance against diseases of enhancing poult poultry；
（2）Bacillus：Synthesize alpha-amylase, protease, lipase, the enzyme such as cellulase, in alimentary canal with animal body
Interior digestive enzymes together plays a role；
（3）Aspergillus niger：Amylase, acid protease, cellulase, pectase, glucose oxidase, citric acid, Portugal can be produced
Saccharic acid and gallic acid etc.；
（4）Aspergillus oryzae：Protease, amylase, carbohydrase, cellulase, phytase etc. can be produced；
（5）Trichoderma viride：A variety of enzyme systems with bioactivity can be produced, such as：Cellulase, chitinase, zytase etc..
The technique effect of the present invention：
The present invention carries out wet ground using string disassembler to raw material first, makes lignocellulosic structure to a certain extent
Loose, inoculation the accessibility of cellulase can be improved after the strain of eccrine fiber element enzyme.In addition, present invention inoculation first can secrete
The composite bacteria of cellulase is fermented, and the cellulase that strain is secreted in fermentation process can degrade the wooden fibre in raw material
Dimension element, and caused monose is available for subsequent fermentation to utilize, and inoculates saccharomycete afterwards and improves crude protein content, this fermentation method
Cellulose is preferably degraded.The present invention provides a kind of economic, efficient mode of production for the production of feed.
【Embodiment】
Embodiment one：Take the mushroom bran 100kg after edible mushroom collection, after wet ground, be inoculated with 10kg composite bacteria liquids（Contain in bacterium solution
Bacillus, aspergillus niger, aspergillus oryzae, Trichoderma viride, saccharomycete, its mass ratio are 3:1:1:1:2）, and 5kg water is added,
0.02kg bitter salts, 0.08kg potassium dihydrogen phosphates, 2kg glucose, 2kg ammonium sulfate, wherein 30 DEG C of fermentations of bacillus
2d, 35 DEG C of aspergillus niger, aspergillus oryzae, Trichoderma viride fermentation 2d, 30 DEG C of fermentation 2d of saccharomycete.
According to this fermentation method, each component content contrast is as follows before and after being fermented：
It is calculated by data, crude protein content adds 51.18% after raw material is fermented, and crude fiber content reduces
15.93%, nutritive value improves.
Embodiment two：Take the mushroom bran 100kg after edible mushroom collection, after wet ground, be inoculated with 8kg composite bacteria liquids（In bacterium solution
Containing bacillus, aspergillus niger, aspergillus oryzae, Trichoderma viride, saccharomycete, its mass ratio is 2:1:1:1:3）, and 5kg water is added,
0.03kg bitter salts, 0.1kg potassium dihydrogen phosphates, 2kg glucose, 2kg ammonium sulfate, 35 DEG C of bacillus fermentation 2d are black
30 DEG C of aspergillus, aspergillus oryzae, Trichoderma viride fermentation 2d, 30 DEG C of fermentation 2d of saccharomycete.
According to this fermentation method, each component content contrast is as follows before and after being fermented：
It is calculated by data, crude protein content adds 36.71% after raw material is fermented, and crude fiber content reduces
10.48%, nutritive value improves.
Embodiment three：Take the mushroom bran 100kg after edible mushroom collection, after wet ground, be inoculated with 10kg composite bacteria liquids（In bacterium solution
Containing bacillus, aspergillus niger, aspergillus oryzae, Trichoderma viride, saccharomycete, its mass ratio is 2:2:2:2:1）, and add 10kg's
Water, 0.03kg bitter salts, 0.1kg potassium dihydrogen phosphates, 1kg glucose, 1kg ammonium sulfate, 30 DEG C of fermentation 4d of bacillus,
30 DEG C of aspergillus niger, aspergillus oryzae, Trichoderma viride fermentation 2d, 30 DEG C of fermentation 2d of saccharomycete.
According to this fermentation method, each component content contrast is as follows before and after being fermented：
It is calculated by data, crude protein content adds 24.7% after raw material is fermented, and crude fiber content reduces
14.62%, nutritive value improves.
Claims (8)
- A kind of 1. production method of composite bacteria solid state fermentation mushroom bran production protein feed, it is characterised in that：After edible mushroom collection Mushroom bran, through wet ground, as matrix, inoculation composite bacteria simultaneously adds trophic factors, and solid state fermentation is carried out after adding water, through dry It is dry that protein feed is made.
- 2. the production method of mushroom bran protein feed according to claim 1, it is characterised in that：At the wet-milling to mushroom bran Reason is completed using string disassembler.
- 3. the production method of mushroom bran protein feed according to claim 1, it is characterised in that：By weight, mushroom bran raw material For 80-90 parts, water is 5-10 parts, and strain is 5-10 parts, and trophic factors is 1.7-4.15 parts.
- 4. the production method of mushroom bran protein feed according to claim 3, it is characterised in that：Trophic factors is selected from seven hydrations Magnesium sulfate, potassium dihydrogen phosphate, glucose, the one or more of ammonium sulfate.
- 5. the production method of mushroom bran protein feed according to claim 4, it is characterised in that：Trophic factors by 0.02 ~ 0.03 part of bitter salt, 0.08 ~ 0.12 part of potassium dihydrogen phosphate, 0.8 ~ 2 part of glucose, 0.8 ~ 2 part of ammonium sulfate composition.
- 6. the production method of mushroom bran protein feed according to claim 1, it is characterised in that：The strain of inoculation is by gemma bar Bacterium, aspergillus niger, aspergillus oryzae, Trichoderma viride and saccharomycete composition, its part by weight are 1 ~ 3:1~3:1~3:1~3:1~3.
- 7. the production method of mushroom bran protein feed according to claim 6, it is characterised in that：The bacillus that is inoculated with, The enzyme for being capable of lignocellulose degradation can be secreted after aspergillus niger, aspergillus oryzae, Trichoderma Viride culture, wherein bacillus is from alkali Property lignin black liquor be neutralized to separation screening in the liquid after neutral culture and draw.
- 8. the production method of mushroom bran protein feed according to claim 6, its fermentation method are：It is inoculated with first into raw material Bacillus, 2 ~ 4d is cultivated under the conditions of 30 ~ 40 DEG C, afterwards inoculated aspergillus niger, aspergillus oryzae, Trichoderma viride into raw material again, 2 ~ 4d is cultivated under the conditions of 30 ~ 40 DEG C, last inoculation yeast bacterium, 2 ~ 3d is cultivated under the conditions of 28 ~ 32 DEG C.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108552406A (en) * | 2018-03-05 | 2018-09-21 | 吉林农业科技学院 | A kind of method of multi-cultur es combined fermentation mushroom bran |
| CN115777843A (en) * | 2022-12-07 | 2023-03-14 | 四川生力源生物工程有限公司 | Preparation method of plant protein fermented feed |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101718049A (en) * | 2009-10-30 | 2010-06-02 | 邹平县海华纸业有限公司 | Clean pulping process for straws by mechanical method |
| CN104171759A (en) * | 2014-09-09 | 2014-12-03 | 中国科学院天津工业生物技术研究所 | Edible mushroom residue feed preparation process |
| CN104336412A (en) * | 2014-11-11 | 2015-02-11 | 武汉轻工大学 | Method for producing monogastric animal feed by fermenting pleurotus eryngii bran |
| CN104472859A (en) * | 2014-12-29 | 2015-04-01 | 江苏千药堂国医研究院有限公司 | Biologically fermented fungus bran protein feed additive |
| CN104872378A (en) * | 2015-04-30 | 2015-09-02 | 北京中生奥普寡肽技术研究所 | Method for preparing high-protein feed by using baijiu vinasse |
| JP6127261B2 (en) * | 2015-01-28 | 2017-05-17 | 株式会社鎌田工業 | Bird feed |
-
2017
- 2017-09-19 CN CN201710847086.5A patent/CN107494928A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101718049A (en) * | 2009-10-30 | 2010-06-02 | 邹平县海华纸业有限公司 | Clean pulping process for straws by mechanical method |
| CN104171759A (en) * | 2014-09-09 | 2014-12-03 | 中国科学院天津工业生物技术研究所 | Edible mushroom residue feed preparation process |
| CN104336412A (en) * | 2014-11-11 | 2015-02-11 | 武汉轻工大学 | Method for producing monogastric animal feed by fermenting pleurotus eryngii bran |
| CN104472859A (en) * | 2014-12-29 | 2015-04-01 | 江苏千药堂国医研究院有限公司 | Biologically fermented fungus bran protein feed additive |
| JP6127261B2 (en) * | 2015-01-28 | 2017-05-17 | 株式会社鎌田工業 | Bird feed |
| CN104872378A (en) * | 2015-04-30 | 2015-09-02 | 北京中生奥普寡肽技术研究所 | Method for preparing high-protein feed by using baijiu vinasse |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108552406A (en) * | 2018-03-05 | 2018-09-21 | 吉林农业科技学院 | A kind of method of multi-cultur es combined fermentation mushroom bran |
| CN115777843A (en) * | 2022-12-07 | 2023-03-14 | 四川生力源生物工程有限公司 | Preparation method of plant protein fermented feed |
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