CN106417898A - Preparation method of microbiological preparation capable of increasing utilization rate of cotton straw and cottonseed hull feed, and application of microbiological preparation - Google Patents
Preparation method of microbiological preparation capable of increasing utilization rate of cotton straw and cottonseed hull feed, and application of microbiological preparation Download PDFInfo
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- CN106417898A CN106417898A CN201610836801.0A CN201610836801A CN106417898A CN 106417898 A CN106417898 A CN 106417898A CN 201610836801 A CN201610836801 A CN 201610836801A CN 106417898 A CN106417898 A CN 106417898A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
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Abstract
The invention belongs to the field of processing and researching of feeds in zootechny, and particularly relates to a microbiological preparation capable of increasing the utilization rate of cotton straw and cottonseed hulls. The microbiological preparation consists of microbiological powder A and an additive B. A method of applying the microbiological preparation to treatment of the cotton straw and the cottonseed hulls comprises the following steps of (1) taking 30-50 parts of the additive B, adding 500-2000 parts of crushed cotton straw and/or cottonseed hulls, performing uniform mixing, sprinkling clean water according to the water content of 60-70%, performing uniform mixing, performing heaping to obtain a small pile, performing covering with a shading net, and performing natural stacking for 7 days; and (2) adding 10-50 parts of the microbiological powder A, performing uniform mixing, paving a mixture into a bar-shaped structure which is 10cm high and 30cm wide, performing covering with the shading net, performing natural stacking for 30 days, and during stacking, sprinkling the clean water every day to maintain the moisture content of the mixture between 60-70%, so that a feed mainly containing the cotton straw and/or the cottonseed hulls can be obtained. After the cotton straw and/or the cottonseed hulls treated by the method are fed to sheep, the utilization rate of the cotton straw and/or the cottonseed hulls can be notably increased.
Description
Technical field
The invention belongs to Animal Science feed manufacturing research field, more particularly to a kind of for improving cotton stalk and Semen Gossypii
Shell is as the preparation method of the microorganism formulation of the utilization rate of feedstuff and its for improving cotton stalk and cotton seed hullss feed conversion
The application of rate.
Background technology
Cotton stalk and cotton seed hullss contain the energy of more than half accumulated by photosynthesis of plant, rich in raw sugar, thick egg
White matter etc. is conducive to the composition needed for domestic animals and fowls, for feeding poultry, is particularly used for feeding the ruminant domestic animals such as cattle, sheep, still
Can so become the feed resource of high-quality, but because its wood fibre cellulose content is high, containing free gossypol and palatability difference etc. limit because
Element, is directly used as that efficiency of feed utilization is low, effect is poor, hinders the comprehensive utilization of cotton stalk and cotton seed hullss scale.Microorganism
Facture can be acted on by fermentable, can be divided the cellulose in cotton stalk and cotton seed hullss, hemicellulose and lignin
Solution becomes monosaccharide, disaccharide, is further oxidized to CO2, feed after domestic animal can improve cotton stalk and cotton seed hullss digestibility or
DM in the rumen degradation rate, so as to improve the utilization rate of cotton stalk and cotton seed hullss.
Content of the invention
It is an object of the invention to：By researching and developing a kind of microorganism formulation for processing cotton stalk and cotton seed hullss, use
After the feedstuff of prepared by this microorganism formulation mainly contain cotton stalk and cotton seed hullss is delivered to the domestic animals such as sheep, significantly can carry
The domestic animals such as high sheep are to cotton stalk and the utilization rate of cotton seed hullss.
Technical scheme：1st, the microorganism formulation of cotton stalk and cotton seed hullss utilization rate, the microorganism system are improved
Agent is made up of micro organism powder A and additive B, and wherein, the preparation method of described micro organism powder A is：By whiterot fungi, black
Aspergillosiss, gibberella, rod method mycete and penicillium at least two or two or more combination 0.5-1 parts are inoculated in and have sterilized
In 50-100 part PD culture fluid, shaken cultivation 5 days, prepared seed liquor under conditions of 25 DEG C, 150r/min；Seed liquor is used
Sterilized water is serially diluted to 10-3After CFU/mL, take 5-10 part diluent and be inoculated in 100-300 part PD culture fluid, 25
DEG C, by strain culturing to logarithmic (log) phase under conditions of 150r/min, draw 40-80 part culture fluid and 20-60 part Testa Tritici, 10-40 part
Bean cake, 10-40 part Semen Maydis powder, 5-10 part defatted rice bran, 5-10 part Zein powder are mixed, will be cold for the microbial mixture for mixing
Micro organism powder A being obtained after lyophilizing is dry, its viable count is determined for 1.9 × 108CFU/g, loads in tinfoil paper paper bag, seals after evacuation
Mouthful, it is placed in 4 DEG C of preservations；The additive B is made up of quick lime 10-25 part, sucrose 10-15 part.
2nd, a kind of above-mentioned microorganism formulation is applied in cotton stalk and cotton seed hullss feedstuff, under the application process includes
State step：(1) taking additive B 30-50 part adds the cotton stalk of 500-2000 part crushing and/or cotton seed hullss to mix, by 60%
～70% water content sprinkling clean water, mix homogeneously, little pile type is piled, shade net is covered, stack 7 days naturally；(2) to step
(1) 10-50 part micro organism powder A being added in, mixes, mixture is paved into high 10 centimetres, wide 30 centimetres list structure, is covered
Shade net, stacks 30 days naturally, and period sprays clean water daily on strip mixture, keeps the moisture in mixture
Between 60%～70%；The feedstuff for mainly containing cotton stalk and/or cotton seed hullss can be obtained.
In above-mentioned, whiterot fungi, Aspergillus niger, gibberella, rod method mycete, penicillium, Testa Tritici, bean cake, Semen Maydis powder, defat
Testa oryzae, Zein powder, quick lime, sucrose, cotton stalk and cotton seed hullss are commercially available.
Beneficial effects of the present invention：In sheep as experimental animal, to feed microbial treatments under same trophic level
Cotton stalk afterwards and/cotton seed hullss, carry out Digestion And Metabolism In Sheep test, weigh surplus material, the urine of collection sheep, feces, determine
Fiber content in sheep concentrate, cotton stalk and/cotton seed hullss, show by analyzing the data obtained, feeding this product substantially can be carried
High cotton stalk and cotton seed hullss utilization rate.The mechanism of action：Cellulose, hemicellulose and wooden in cotton stalk and/cotton seed hullss
Element in microorganism under the synergism of beta-glucosidase, exoglucanase, endoglucanase, can be converted into monosaccharide,
Disaccharide, is further oxidized to CO2, fed after sheep with the cotton stalk after the process of this microorganism formulation and/cotton seed hullss,
Experiment shows to significantly improve the utilization rate of cotton stalk and/cotton seed hullss.
Specific embodiment
The present invention is further described in conjunction with the embodiments.
Practical proof：Embodiment 1, feedstuff S1 is prepared, one, prepare micro organism powder A：Respectively by whiterot fungi, Aspergillus niger
Each 0.8 part is inoculated in the 80 parts of PD culture fluid for having sterilized, shaken cultivation 5 days under conditions of 25 DEG C, 150r/min, is obtained
Seed liquor, standby；Seed liquor is serially diluted to 10 using sterilized water-3After CFU/mL, take 7 parts of diluents and be inoculated in 200
In part PD culture fluid, by strain culturing to logarithmic (log) phase under conditions of 25 DEG C, 150r/min, 50 parts of culture fluid and 25 portions of wheats are drawn
Bran, 15 parts of bean cake, 15 parts of Semen Maydis powder, 10 parts of defatted rice brans, 10 parts of Zein powders mix 30-40min, the microorganism that will mix
Micro organism powder A being obtained after mixture lyophilization, its viable count is determined for 1.9 × 108CFU/g, loads in tinfoil paper paper bag, takes out
Seal after vacuum, be placed in 4 DEG C of preservations；2nd, (1) takes 30 parts of cotton stalks for adding 500 parts of crushing of additive B and/or Semen Gossypii
Shell, mixes, and by 60%～70% water content sprinkling clean water, mix homogeneously, piles little pile type, covers shade net, natural heap
Put 7 days；(2) 30 parts of micro organism powder A are added in step (1), mix, by mixture be paved into high 10 centimetres, wide 30 centimetres
List structure, covers shade net, stacks 30 days naturally, and period sprays clean water daily on strip mixture, keeps mixing
Moisture in thing between 60%～70%, prepared feedstuff S1.
Embodiment 2, feedstuff S2 is prepared, one, prepare micro organism powder A：Respectively by whiterot fungi, each 0.8 part of inoculation of gibberella
In the 80 parts of PD culture fluid for having sterilized, shaken cultivation 5 days under conditions of 25 DEG C, 150r/min, prepared seed liquor, standby
With；Seed liquor is serially diluted to 10 using sterilized water-3After CFU/mL, take 7 parts of diluents and be inoculated in 200 parts of PD culture fluid
In, by strain culturing to logarithmic (log) phase under conditions of 25 DEG C, 150r/min, draw 50 parts of culture fluid and 25 parts of Testa Tritici, 15 parts of beans
The dregs of rice, 15 parts of Semen Maydis powder, 10 parts of defatted rice brans, 10 parts of Zein powders mix 30-40min, will be cold for the microbial mixture for mixing
Micro organism powder A being obtained after lyophilizing is dry, its viable count is determined for 1.9 × 108CFU/g, loads in tinfoil paper paper bag, seals after evacuation
Mouthful, it is placed in 4 DEG C of preservations；2nd, (1) takes 30 parts of cotton stalks for adding 1000 parts of crushing of additive B and/or cotton seed hullss, mixes,
By 60%～70% water content sprinkling clean water, mix homogeneously, little pile type is piled, shade net is covered, stack 7 days naturally；(2)
30 parts of micro organism powder A are added in step (1), mix, mixture is paved into high 10 centimetres, wide 30 centimetres list structure,
Shade net is covered, is stacked 30 days naturally, period sprays clean water daily on strip mixture, keeps the moisture in mixture
Content between 60%～70%, prepared feedstuff S2.
Embodiment 3, prepare feedstuff S3：First, micro organism powder A is prepared：Respectively will be each 0.8 part to whiterot fungi, rod method enzyme bacterium
It is inoculated in the 80 parts of PD culture fluid for having sterilized, shaken cultivation 5 days under conditions of 25 DEG C, 150r/min, prepared seed liquor,
Standby；Seed liquor is serially diluted to 10 using sterilized water-3After CFU/mL, take 7 parts of diluents and be inoculated in 200 parts of PD cultures
In liquid, by strain culturing to logarithmic (log) phase under conditions of 25 DEG C, 150r/min, draw 50 parts of culture fluid and 25 parts of Testa Tritici, 15 parts
Bean cake, 15 parts of Semen Maydis powder, 10 parts of defatted rice brans, 10 parts of Zein powders mix 30-40min, and the microorganism of mixing is mixed
Micro organism powder A being obtained after compound lyophilization, its viable count is determined for 1.9 × 108CFU/g, loads in tinfoil paper paper bag, takes out true
Seal after sky, be placed in 4 DEG C of preservations；2nd, (1) takes 30 parts of cotton stalks for adding 2000 parts of crushing of additive B and/or cotton seed hullss,
Mix, by 60%～70% water content sprinkling clean water, mix homogeneously, little pile type is piled, shade net is covered, stack naturally 7
My god；(2) 30 parts of micro organism powder A are added in step (1), mixes, mixture is paved into high 10 centimetres, wide 30 centimetres strip
Structure, covers shade net, stacks 30 days naturally, and period sprays clean water daily on strip mixture, keeps in mixture
Moisture between 60%～70%, prepared feedstuff S3.
Embodiment 4, prepare feedstuff S4：First, micro organism powder A is prepared：Respectively by whiterot fungi, each 0.8 part of inoculation of penicillium
In the 80 parts of PD culture fluid for having sterilized, shaken cultivation 5 days under conditions of 25 DEG C, 150r/min, prepared seed liquor, standby
With.Seed liquor is serially diluted to 10 using sterilized water-3After CFU/mL, take 7 parts of diluents and be inoculated in 200 parts of PD culture fluid
In, by strain culturing to logarithmic (log) phase under conditions of 25 DEG C, 150r/min, draw 50 parts of culture fluid and 25 parts of Testa Tritici, 15 parts of beans
The dregs of rice, 15 parts of Semen Maydis powder, 10 parts of defatted rice brans, 10 parts of Zein powders mix 30-40min, by the microorganism mixing for mixing
Micro organism powder being obtained after thing lyophilization, its viable count is determined for 1.9 × 108CFU/g, loads in tinfoil paper paper bag, evacuation
After seal, be placed in 4 DEG C of preservations；2nd, (1) takes 30 parts of cotton stalks for adding 1500 parts of crushing of additive B and/or cotton seed hullss, mixes
Even, by 60%～70% water content sprinkling clean water, mix homogeneously, little pile type is piled, shade net is covered, stack 7 days naturally；
(2) 30 parts of micro organism powder A are added in step (1), mixes, mixture is paved into high 10 centimetres, wide 30 centimetres strip knot
Structure, covers shade net, stacks 30 days naturally, and period sprays clean water daily on strip mixture, keeps in mixture
Moisture between 60%～70%, prepared feedstuff S4.
Implement component design and compliance test result for feedstuff S1, S2, S3 and S4：
1 materials and methods
1.1 test periods and place
This is tested was carried out in Xinjiang Huaxing breeding stock Breeding Center in May, 2016 in August, 2016.
1.2 experimental animals and EXPERIMENTAL DESIGN
The close Tao Saite ewe 50 of test selection age, body weight, is randomly divided into 5 groups, respectively matched group, test I group, examination
Test II group, III group of test and IV group of test.Matched group and test group pottery Saite ewe feed same trophic level and same dose
Concentrate.Per only ewe every morning 07:00 and afternoon 17:00 feeds 250g concentrate respectively, after concentrate feeding is finished, control
Group, I group of test, II group of test, III group of test and IV group of test are respectively at daily 07:00 and afternoon 17:The common Cotton Gossypii of 00 feeding
Straw, each 1kg of feedstuff S1, S2, S3 and S4, while ensure sufficient drinking-water.The digest supersession test of 20 days by a definite date is carried out, wherein
13 days preliminary trial periods, just trying the phase 7 days.
The collection of 2 samples and measure
Just trying in the phase, to make ewe whole day keep standing state, using self-control urine collection apparatus, whole day collects ewe urine and excrement per 2h
Sample once, whole day urine is fully shaken up, and is measured cumulative volume with graduated cylinder and is taken 10%, is stored in plastics after the concentrated sulphuric acid for adding 5%
Bottle, Seal and preservation simultaneously makes a record, and the urine sample that test ewe is collected for 7 days is mixed, and takes 1L preservation to be measured；The whole day excrement that will collect
Sample is mixed completely, and the 10% of random crawl excrement sample gross weight, it is put in numbered sample sack, weighs after natural air drying.By ewe
The excrement sample of continuous 7 days natural air dryings is mixed, take 1kg perform record seal up for safekeeping to be measured；While collecting concentrate, common cotton stalk, feeding
Material S1, S2, S3 and S4.
In concentrate, common cotton stalk, feedstuff S1, S2, S3 and S4, excrement sample and urine sample, dry, Organic substance, calcium, phosphorus contain
Amount is all measured using conventional feed analysis method；Neutral detergent fiber and acid detergent fiber content adopt U.S. ANKOM
Fibre analyser is measured；Gross protein value is surveyed using the quick azotometer of Germany Elementar Analysen Systeme
Fixed.
3 data analysiss
Result of the test is represented with average (Mean) ± standard deviation (SD).Test data all carries out variance using SPSS18.0 software
Analysis.Result of the test carries out multiple comparisons using Duncan.
4 results and analysis
The impact of feeding feed stuff S1, S2, S3 and S4 to Tao Saite ewe nutritive digestibility is shown in Table 1.
The different lowercase letter indication differences of colleague's data shoulder mark are notable (P ＜ 0.05), and the different capitalizations of shoulder mark represent poor
Heteropole is notable (P ＜ 0.01), and shoulder mark same letter or no letter represent that difference is not notable (P ＞ 0.05).
As shown in Table 1, each test group dry, organic matter digestibility are above matched group, wherein test III group and reach
Pole significant level (P ＜ 0.01)；Each group thick protein digestibility difference is notable (P ＞ 0.05), but each test group be above right
According to group；Each test group cellulose digestibility is above matched group, wherein tests II group of pole and is significantly higher than matched group (P ＜ 0.01),
Test I group, test II group, test III group, test IV group of cellulose digestibility be higher by 8.17% (P ＞ than matched group respectively
0.05), 11.22% (P ＜ 0.01), 6.75% (P ＞ 0.05), 8.01% (P ＞ 0.05)；Test I group of hemicellulose digestibility
Matched group (P ＜ 0.05) is significantly higher than, I group of test, II group of test, III group of test, IV group of hemicellulose digestibility of test are respectively
13.20% (P ＜ 0.05), 11.71% (P ＜ 0.05), 5.73% (P ＞ 0.05), 7.96% (P ＞ are higher by than matched group
0.05)；Each group calcium, phosphorus digestibility difference is not notable (P ＞ 0.05), but each test group calcium, phosphorus digestibility are above matched group.
Claims (2)
1. a kind of microorganism formulation for improving cotton stalk and cotton seed hullss utilization rate, it is characterised in that：The microorganism formulation be by
Micro organism powder A and additive B composition, wherein, the preparation method of described micro organism powder A is：By whiterot fungi, aspergillus niger
Bacterium, gibberella, rod method mycete and penicillium at least two or two or more combination 0.5-1 parts are inoculated in the 50- for having sterilized
In 100 parts of PD culture fluid, shaken cultivation 5 days, prepared seed liquor under conditions of 25 DEG C, 150 r/min；By seed liquor using no
Bacterium water is serially diluted to 10-3After CFU/mL, take 5-10 part diluent and be inoculated in 100-300 part PD culture fluid, 25
DEG C, by strain culturing to logarithmic (log) phase under conditions of 150 r/min, draw 40-80 part culture fluid and 20-60 part Testa Tritici, 10-40 part
Bean cake, 10-40 part Semen Maydis powder, 5-10 part defatted rice bran, 5-10 part Zein powder are mixed, will be cold for the microbial mixture for mixing
Micro organism powder A being obtained after lyophilizing is dry, its viable count is determined for 1.9 × 108CFU/g, loads in tinfoil paper paper bag, seals after evacuation
Mouthful, it is placed in 4 DEG C of preservations；The additive B is made up of quick lime 10-25 part, sucrose 10-15 part.
2. a kind of microorganism formulation by described in claim 1 is applied in cotton stalk and cotton seed hullss feedstuff, and its feature exists
In the application process comprises the steps：（1）Take additive B 30-50 part add cotton stalk that 500-2000 part crushes and/
Or cotton seed hullss are mixed, by 60% ~ 70% water content sprinkling clean water, mix homogeneously, little pile type is piled, shade net is covered, natural
Stack 7 days；（2）To step（1）Middle addition 10-50 part micro organism powder A, mixes, mixture is paved into high 10 centimetres, wide 30 lis
The list structure of rice, covers shade net, stacks 30 days naturally, and period sprays clean water daily on strip mixture, keeps
Moisture in mixture is between 60% ~ 70%；The feedstuff for mainly containing cotton stalk and/or cotton seed hullss can be obtained.
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Cited By (3)
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| CN110313542A (en) * | 2019-08-06 | 2019-10-11 | 塔里木大学 | A method of fermented feed is prepared using cotton byproduct |
| CN110637919A (en) * | 2019-09-18 | 2020-01-03 | 新和县园邦城农业发展有限公司 | Novel straw composite feed of yellow mealworm sand and ghost |
| CN110833115A (en) * | 2019-11-21 | 2020-02-25 | 南京胜昔实业有限公司 | Comprehensive utilization method of cotton production by-products by using biochemical fulvic acid as main product |
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