CN110313347A - Oyster mushroom culture medium and preparation method thereof containing walnut beta pruning sawdust - Google Patents
Oyster mushroom culture medium and preparation method thereof containing walnut beta pruning sawdust Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
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Abstract
The invention belongs to technical field of edible fungi cultivation, and in particular to the Oyster mushroom culture medium and preparation method thereof containing walnut beta pruning sawdust.Using mycelial growth rate and the method for enzymatic activity, screening, which obtains, applies the big oyster mushroom kind A of walnut beta pruning sawdust potentiality.Oyster mushroom A cultivation matrix material component of the invention: walnut beta pruning sawdust 63, cotton seed hull 30, wheat bran 5, gypsum 2 can effectively improve the biological transformation ratio and yield of oyster mushroom A.Oyster mushroom A cultivation matrix material component: walnut beta pruning sawdust 90, wheat bran 8, gypsum 2 can effectively improve the content of crude fibre in mushroom carpophore, protein, fat, VC and amino acid.The popularization and application of cultivation matrix of the present invention can alleviate Hebei province's sawdust resource shortage significantly, reduce oyster mushroom input cost, obtain the functional oyster mushroom that consumer needs, have a extensive future, economic benefit is huge.
Description
Technical field
The invention belongs to technical field of edible fungi cultivation, and in particular to Yield of Pleurotus Ostreatus can be improved, biological transformation ratio, improve son
The cultivation matrix of the oyster mushroom kind A containing walnut beta pruning sawdust of the nutritional ingredients such as amino acid, albumen, crude fibre, VC in entity
And preparation method thereof.
Background technique
Oyster mushroom (scientific name: Pleurotus ostreatus；Also known as pick up the ears, oyster cap fungus, oyster mushroom, black tree peony mushroom, Taiwan is again
Claim elegant precious mushroom) it is one type of Agaricales Pleurotaceae under Basidiomycota, it is that kind of a fairly common grey eats mushroom, Chinese medicine thinks oyster mushroom
It is warm-natured, sweet in flavor.Have effects that wind-evil dispelling and cold-evil expelling, relaxing tendons and activating collaterals, can be used for lumbocrural pain, the illnesss such as numb in every limb, grain is obstructed.
With people's living standard and the raising required quality of life, the consumption figure of edible mushroom increases year by year, and market is huge
Greatly, development prospect is wide.Edible mushroom relies on stronger lignin, cellulose degradation ability, for agricultural, the resource of trade waste
Change using the effective way of environmental protection, Ecological Sustainable Development is provided, increases the application added value of waste, promote circulation
Expanding economy.Traditional edible fungus culturing raw material includes cotton seed hull, sawdust etc..With the expansion of industry size, to cotton seed hull,
The demand of the raw materials such as sawdust is growing day by day.On the other hand, as the sharply reduction of our province sown areas of cotton is related to forestry
The influence of policy, the raw material supplies amount such as cotton seed hull, sawdust are increasingly reduced, and cause the prices of raw materials soaring, have been seriously affected edible
The income of bacterium production.Therefore it is badly in need of seeking one of the precondition that edible fungus culturing new raw material develops in a healthy way as promotion industry.
Hebei province, which is that walnut plantation is big, to be saved, and for cultivated area up to more than 300 ten thousand mu, beta pruning sawdust total amount may be up to 900,000 every year
Ton, it is rich in lignin, cellulose in branch, is ground into sawdust and is suitable as edible fungus culturing raw material.
So far, the prior art there are no the report using walnut beta pruning sawdust as Oyster mushroom culture medium.
Summary of the invention
The present invention is to solve the problems, such as Hebei province's weed tree sawdust shortage of resources rise in price and lack functional cultivation matrix, is mentioned
For the cultivation matrix and preparation method thereof of the oyster mushroom kind A containing walnut beta pruning sawdust.It can solve to lead because of broad-leaved wood chip valence height
The problem of mushroom cultivation cost of cause rises steadily promotes the resource utilization of walnut beta pruning sawdust；Walnut beta pruning sawdust adds
Add the raising of the increase, biological transformation ratio that can promote Yield of Pleurotus Ostreatus, improves crude fibre, albumen, polysaccharide, amino acid etc. in fructification
Nutritional ingredient keeps the nutrient functional of oyster mushroom kind A fructification bigger, provides by force for the further research and development of functional cultivation matrix
Strong basis.
Purpose to realize the present invention, the invention adopts the following technical scheme:
A kind of Oyster mushroom culture medium containing walnut beta pruning sawdust of the present invention, includes following material component by weight:
Walnut beta pruning sawdust 33~90, corncob 0~30, cotton seed hull 0~30, wheat bran 5~8, gypsum 2.
Preferably, the Oyster mushroom culture medium containing walnut beta pruning sawdust, the oyster mushroom are oyster mushroom kind A
Preferably, the Oyster mushroom culture medium containing walnut beta pruning sawdust includes following material component by weight:
Walnut beta pruning sawdust 63, cotton seed hull 30, wheat bran 5, gypsum 2.
Preferably, the Oyster mushroom culture medium containing walnut beta pruning sawdust includes following material component by weight:
Walnut beta pruning sawdust 90, wheat bran 8, gypsum 2.
The Oyster mushroom culture medium containing walnut beta pruning sawdust, the oyster mushroom A of the formula cultivation, biological transformation ratio reach
117.75%, output increased 48.71%, in fructification crude fiber content improve 16.67%, protein content improve 17.24%,
Polyoses content improves 25%, fat content and improves 66.67%, VC content raising 5.38%.
Preferably, the oyster mushroom A of the formula cultivation, biological transformation ratio is up to 90.92%, and output increased 14.83%, son is in fact
Crude fiber content improves 33.33%, protein content raising 25.86% in body, fat content improves 66.67%, VC content and mentions
High 2.69%, total amino acid content improves 36.26%, and wherein isoleucine, leucine and phenylalanine content are respectively increased
230%, 163.15% and 131.30%.
A kind of preparation method of the Oyster mushroom culture medium containing walnut beta pruning sawdust, comprising the following steps:
A. walnut beta pruning is ground into the particle that partial size is 3~8mm with pulverizer, is impregnated 12~24 hours, is made with clear water
Walnut beta pruning sawdust is sufficiently drenched；
B. first moisture extra in walnut beta pruning sawdust is leached, then adds respective substance to mixing by material proportion
Machine, batch mixer stir 20~30 minutes, and water content is added water in mixing process up to 60~80%；
C. mixed material is packed, sterilizes 2 hours at 121 DEG C, after sterilizing at 50~60 DEG C of the temperature of bacterium bag, is transported
In the inoculation environment low to bacteria containing amount, the preparation of completion oyster mushroom kind A cultivation matrix, 12-28 hours to 10~15 DEG C to be cooled
When be quickly inoculated with, growth 33~38 days after oyster mushroom kind A.
Preferably, the preparation method of the Oyster mushroom culture medium containing walnut beta pruning sawdust, in the step c, in the summer
Season twice, sterilizes the sterilizing of mixed material interruption 2 hours every time.
The preparation method of the Oyster mushroom culture medium containing walnut beta pruning sawdust, comprising the following specific steps
Walnut beta pruning is ground into the particle that partial size is 5mm with pulverizer, is impregnated 18 hours with clear water, keeps walnut beta pruning wooden
Bits are sufficiently drenched；Water is drained every other day, respective substance is added to batch mixer by material proportion, stirs 20~30 points in batch mixer
Clock, when adding water to water content in mixing process up to 65~70%, pack sterilizes 2~4 hours at 121 DEG C, after sterilizing,
It at 50~60 DEG C of the temperature of bacterium bag, ships it in the low inoculation environment of bacteria containing amount, completes the system of oyster mushroom kind A cultivation matrix
It is standby, it is quickly inoculated with when (12-28 hours) to be cooled are to 10~15 DEG C.Oyster mushroom kind A is obtained after growth 33~38 days.
The material proportion includes: walnut beta pruning sawdust 63, cotton seed hull 30, wheat bran 5, gypsum 2 by weight.
The above-mentioned preparation method for stating the Oyster mushroom culture medium containing walnut beta pruning sawdust, the material proportion is by weight
Are as follows: walnut beta pruning sawdust 90, wheat bran 8, gypsum 2.
The substantive distinguishing features outstanding and effect that the present invention has compared with the existing technology:
1, the present invention use walnut beta pruning sawdust medium measure mycelial growth rate method and walnut beta pruning sawdust for
The method of substrate measurement laccase activity combines, it is determined that using the screening technique of the oyster mushroom kind of walnut beta pruning sawdust, obtains
Obtained an optimal oyster mushroom kind A；
2, the present invention is substrate culture oyster mushroom using walnut beta pruning sawdust, can effectively solve to lead because of broad-leaved wood chip shortage of resources
Cause price soaring the problem of causing mushroom grower income to reduce；Hebei province is promoted to discard the higher value application of walnut beta pruning sawdust resource；
3, the cultivation matrix containing walnut beta pruning sawdust can promote the fast-growth of oyster mushroom kind A mycelia, when shortening purseful
Between, it is conducive to reduce contamination rate；Increase biological transformation ratio, improve Yield of Pleurotus Ostreatus, increases mushroom grower income；
4, the quality of oyster mushroom can be improved in the addition of walnut beta pruning sawdust, increases albumen, polysaccharide, crude fibre, rouge in fructification
The content of fat and amino acid, the raising of crude fiber content are conducive to meet the dietary requirements of diabetes patient；The raising benefit of polyoses content
In the immunity for improving human body；The raising of fat content can meet the needs of middle-aged and the old is to plant fat.
5, the cultivation medium formula of the oyster mushroom kind A provided by the invention containing walnut beta pruning sawdust is simple, and raw material is easy to get,
Production cost is low, has a extensive future, and has very big rush to the development for promoting Walnut Industry, mushroom industry and functional food
Into effect.
Detailed description of the invention
Fig. 1 is the growth conditions for testing oyster mushroom kind A mycelia in control medium in 1；
Fig. 2 is the growth conditions for testing oyster mushroom kind A mycelia on culture medium 1 in 1；
Fig. 3 is the growth conditions for testing oyster mushroom kind A mycelia on culture medium 2 in 1；
Fig. 4 is the growth conditions for testing oyster mushroom kind A mycelia on culture medium 3 in 1；
Fig. 5 is laccase activity of the oyster mushroom kind A at 5 DEG C in experiment 2；
Fig. 6 is laccase activity of the oyster mushroom kind A at 10 DEG C in experiment 2；
Fig. 7 is laccase activity of the oyster mushroom kind A at 15 DEG C in experiment 2；
Fig. 8 is laccase activity of the oyster mushroom kind A at 20 DEG C in experiment 2；
Fig. 9 is laccase activity of the oyster mushroom kind A at 25 DEG C in experiment 2；
Figure 10 is laccase activity of the oyster mushroom kind A at 30 DEG C in experiment 2；
Specific embodiment:
With reference to the accompanying drawing and specific embodiment the present invention is further illustrated.
Experiment 1
Mycelial growth rate method screening can utilize the oyster mushroom kind of walnut beta pruning sawdust
A1. utilize mycelial growth rate detection method, to No. 5 four oyster mushroom kinds of oyster mushroom A, 2028,650 and De Feng into
The measurement of row mycelial growth rate, to obtain the oyster mushroom kind that can utilize walnut beta pruning sawdust.Test is using walnut beta pruning wood
Consider 4 kinds of different culture mediums of content to be worth doing, specific formula is as follows:
Control medium: glucose 20g, magnesium sulfate 1.5g, potassium dihydrogen phosphate 0.3g, peptone 0.5g, vitamin 2,
PH is naturally, agar powder 1.2%, 1L；
Culture medium 1: magnesium sulfate 1.5g, potassium dihydrogen phosphate 0.3g, peptone 0.5g, vitamin 2, walnut beta pruning sawdust
200g, pH are naturally, agar powder 1.2%, 1L；
Culture medium 2: glucose 20g, magnesium sulfate 1.5g, potassium dihydrogen phosphate 0.3g, vitamin 2, walnut beta pruning sawdust
200g, pH are naturally, agar powder 1.2%, 1L；
Culture medium 3: magnesium sulfate 1.5g, potassium dihydrogen phosphate 0.3g, vitamin 2, walnut beta pruning sawdust 200g, pH naturally,
Agar powder 1.2%, 1L；
Configuration method according to above 4 kinds of culture mediums prepares culture medium, 121 DEG C of sterilizing 30min, using training after slightly cooling down
Base automatic sub-packaging device is supported, the culture medium of 30ml is added in each quantitatively plate.It is after culture medium solidification, four cultivars are big
Small consistent cake fungus block is inoculated into the center of plate, cultivates 4 days for 25 DEG C in incubator.
A2. oyster mushroom colony diameter is measured, the average daily speed of growth of mycelia is calculated.Seen from table 1, it is handled with control medium
It compares, the culture medium 1 and culture medium 3 of oyster mushroom kind A can promote the growth of hypha of Pleurotus ostreatus, illustrate that walnut beta pruning sawdust can be used as carbon
Source and nitrogen source ensure the normal growth of oyster mushroom；The speed of growth of 1 mycelia of culture medium of oyster mushroom kind 650 is faster than at control medium
Reason；The speed of growth of No. 5 two kind mycelia of oyster mushroom kind 2028 and De Feng is below control medium.Illustrate different oyster mushroom product
Kind is different to the producing level and effect of walnut beta pruning sawdust, fast-growth on oyster mushroom kind A culture medium 1 and culture medium 3, explanation
The potentiality of its cultivating in bag application walnut beta pruning sawdust are larger.By attached drawing 1-4 as it can be seen that oyster mushroom kind A is in culture medium 1 (attached drawing 2)
It is big with the colony diameter on culture medium 3 (attached drawing 4), and mycelia is more sturdy.
The average daily speed of growth of 1 mycelia of table
A3. by the average daily speed of growth of mycelia and attached drawing picture as it can be seen that oyster mushroom kind A utilizes the effect of walnut beta pruning sawdust
Most preferably, carbon source and nitrogen source can be provided for its growth, promotes the growth of mycelia and sturdy, has application potential.
Experiment 2
Laccase activity screening can utilize the oyster mushroom kind of walnut beta pruning sawdust
B1. macromolecular lignin degradation can be small molecule nutriment by laccase, conducive to the decomposition benefit to culturing raw material
With activity is higher, and its ability for decomposing lignin is stronger.Using quantitative analysis method, it is sieved walnut beta pruning sawdust to obtain size
80 mesh powder are stand-by.
It configures laccase activity and measures culture medium: walnut beta pruning sawdust 20g, sucrose 1g, guaiacol 2g, 12g agar powder adds
Water is bottled to 1L；In 115 DEG C of sterilizing 30min, after culture medium is slightly cooling, using culture medium automatic sub-packaging device, each
The culture medium of 13ml is added in quantitative plate, it is after culture medium is cooling, No. 5 four kinds of oyster mushrooms of oyster mushroom A, 2028,650 and De Feng are big
Small consistent cake fungus block is linked on plate, is placed in the training of 5 DEG C, 10 DEG C, 15 DEG C, 20 DEG C, 25 DEG C and 30 DEG C of incubator
It supports four days.
B2. the result of measurement discoloration loop diameter is as can be seen from Table 2, oyster mushroom 650 and De Feng 5 raisings with cultivation temperature, more
The activity of phenol oxidase increases, enzymatic activity highest at 30 DEG C；When oyster mushroom kind A and 2,028 two kinds are between 5 DEG C to 25 DEG C,
The activity of polyphenol oxidase constantly increases, and at 30 DEG C, the activity of enzyme has the tendency that reduction.But oyster mushroom kind A 5-30 DEG C it
Between, it is the kind of one wide temperature that the laccase activity at each temperature, which is above No. 5 three oyster mushroom kinds of 2028,650 and De Feng,
Potentiality using walnut beta pruning sawdust are big.By attached drawing 5-10 as it can be seen that the size of the laccase activity circle of oyster mushroom kind A, it is seen that with temperature
The raising discoloration circle of degree is gradually increased, and discoloration circle when 25 DEG C is maximum, and the activity of laccase is most strong.
The activity of the polyphenol oxidase of 2 four kinds of oyster mushrooms of table
B3. the laccase activity size of No. 5 four kinds of oyster mushroom kinds of oyster mushroom A, 2028,650 and De Feng relatively shows oyster mushroom kind
The activity of A is maximum, maximum using the potentiality of walnut beta pruning sawdust.
Experiment 3
The preparation method of Oyster mushroom culture medium, comprising the following steps:
C1. weedtree and walnut beta pruning are ground into the particle that partial size is 3~8mm with pulverizer, it will be required according to proportion
Weed tree sawdust and walnut beta pruning sawdust weigh up Hou Duidui, and in turning, sawdust is drenched in water drenching, and the volume ratio of clear water and sawdust is 3~
4:1 is drenched turning with clear water, is covered 12~24 hours with plastic film respectively, is kept weed tree sawdust and walnut beta pruning sawdust sufficiently wet
Thoroughly；Second day again turning make the water content of sawdust up to 90~100%, by moisture extra in weed tree sawdust and walnut beta pruning sawdust
It leaches, is stirred 20~30 minutes with other material mixings；
C2. it is mixed when making the final water content of mixture up to 60~80%, mixture is packed, it is small in 121 DEG C of sterilizings 2
When (summer sterilize 4 hours), after sterilizing when bacterium bag temperature is in 50~60 DEG C, ship it in the low inoculation environment of bacteria containing amount,
It is quickly inoculated with when being cooled to 10~15 DEG C, to reduce the intrusion of miscellaneous bacteria, can effectively reduce contamination rate.
Reference examples:
30 parts of corncob, 33 parts of weed tree sawdust, 30 parts of cotton seed hull, 5 parts of wheat bran, 2 parts of gypsum.
Embodiment 1
30 parts of corncob, 33 parts of walnut beta pruning sawdust, 30 parts of cotton seed hull, 5 parts of wheat bran, 2 parts of gypsum.
Embodiment 2
63 parts of walnut beta pruning sawdust, 30 parts of cotton seed hull, 5 parts of wheat bran, 2 parts of gypsum.
Embodiment 3
30 parts of corncob, 63 parts of walnut beta pruning sawdust, 5 parts of wheat bran, 2 parts of gypsum.
Embodiment 4
90 parts of walnut beta pruning sawdust, 8 parts of wheat bran, 2 parts of gypsum.
Oyster mushroom kind A is carried out walnut beta pruning sawdust difference Bag Material formula test, as a result seen from table 3,1 He of embodiment by C3
The average daily speed of growth of mycelia of oyster mushroom kind A is higher than reference examples in embodiment 2, and 4.43% He is respectively increased in the speed of growth
12.12%；Embodiment 2 can effectively shorten the purseful time, can shorten 5 days；It is real in addition to 3 single bags of yield of embodiment are lower than reference examples
Single bag of yield for applying example 1, embodiment 2 and embodiment 4 is above reference examples, and 16.19%, 48.71% and is respectively increased in yield
14.83%；The biological transformation ratio of embodiment 1, embodiment 2 and embodiment 4 is higher than reference examples, increase rate is respectively 16.18%,
48.71% and 14.83%, wherein the biological transformation ratio of embodiment 2 is up to 117.75%, single bag of yield maximum.
The average daily speed of growth of mycelia, single bag of yield and the biological transformation ratio of the different Bag Material processing of table 3
Test influence of the 4 walnut beta pruning cultivation on sawdust matrix to oyster mushroom kind A crude fibre, protein, polysaccharide and fat
First batch of oyster mushroom of different embodiment processing oyster mushroom kind A in acquisition experiment 3, measures thick fine in new fresh sporophore
The content of dimension, protein, polysaccharide and fat.By table 4 as it can be seen that in addition to embodiment 3, embodiment 1, embodiment 2 and embodiment 4 are fresh
Coarse-fibred content is above reference examples in fructification, and increase rate is respectively 33.33%, 16.67% and 33.33%；Walnut is cut
The addition of branch wood chips can improve the content of protein and fat in fructification, egg in embodiment 1-4 fructification to a certain extent
White matter and fat content are higher than reference examples, and protein is respectively increased 12.64%, 17.24%, 9.20% and 25.86%, fat point
Indescribably high 66.67%, 66.67%, 33.33% and 66.67%；The content of polysaccharide is higher than in 2 fructification of embodiment 1 and embodiment
6.25% and 25% is respectively increased in reference examples；VC content is above control, increase rate in embodiment 1, embodiment 2 and embodiment 4
Respectively 12.69%, 5.38% and 2.69%.As it can be seen that adding walnut beta pruning sawdust in cultivation matrix, can be improved thick fine in sub- reality
The content of dimension, protein, polysaccharide and fat, wherein the content of crude fibre and fat raising degree is higher, can be functional for production
The cultivation of edible health bacterium provides theoretical foundation.
The nutritional ingredient of 4 different disposal oyster mushroom kind A of table compares (100g fresh weight)
Test influence of the 5 walnut beta pruning cultivation on sawdust matrix to amino acid content in oyster mushroom kind A
The content of 16 kinds of amino acid (be shown in by result in first batch of new fresh sporophore that different embodiments are handled in measurement experiment 3
Table 6), in addition to embodiment 2, the total amino acid content of embodiment 1, embodiment 3 and embodiment 4 three processing is higher than reference examples, explanation
The addition of walnut beta pruning sawdust increases the content of amino acid in fructification.7 kinds of amino acid needed by human in 16 kinds of amino acid
For threonine, valine, methionine, isoleucine, leucine, phenylalanine, lysine, embodiment 2 handle in fructification this 7
The content of kind amino acid is below embodiment 1, embodiment 3 and embodiment 4；Valine, methionine content in embodiment 1 is high
In other processing, increase rate is 61.62% and 20%；Isoleucine, leucine and phenylalanine content are above in embodiment 4
Other processing, increase rate 230%, 163.15% and 131.30%；Serine content in reference examples is up to 0.074mg/
100g；Lysine content highest in embodiment 3, increase rate is 8.70% compared with the control.As it can be seen that walnut beta pruning sawdust difference adds
Influence of the dosage to amino acid content is different.
6 different disposal mushroom aminoacid ingredient of table compares (mg/100g)
In conclusion on walnut beta pruning sawdust medium mycelial growth rate it is fast, using walnut beta pruning sawdust as the enzyme of substrate
High oyster mushroom A living, the average daily speed of growth of mycelia is fast in different cultivation matrixes, biological transformation ratio is high, also high single bag of yield
It is high.The content of crude fibre in fructification, protein, polysaccharide and fat can be improved in the addition of walnut beta pruning sawdust.The production of embodiment 2
Amount and biotransformation efficiency highest, can be improved the part nutritional ingredient in fructification；Embodiment 4 to albumen in mushroom carpophore,
The content of the nutritional ingredients such as crude fibre, polysaccharide and isoleucine, leucine and phenylalanine influences maximum.As it can be seen that same oyster mushroom
Kind uses different cultivation matrixes, and being different in terms of yield, quality and nutritional ingredient respectively has advantage and disadvantage, can be according to reality
It needs to select suitable cultivation matrix.
Claims (10)
1. a kind of Oyster mushroom culture medium containing walnut beta pruning sawdust, characterized in that by weight include following material component:
Walnut beta pruning sawdust 33~90, corncob 0~30, cotton seed hull 0~30, wheat bran 5~8, gypsum 2.
2. the Oyster mushroom culture medium as described in claim 1 containing walnut beta pruning sawdust, characterized in that the oyster mushroom is oyster mushroom product
Kind A.
3. the Oyster mushroom culture medium as described in claim 1 containing walnut beta pruning sawdust, characterized in that include by weight with
Lower material component: walnut beta pruning sawdust 63, cotton seed hull 30, wheat bran 5, gypsum 2.
4. the Oyster mushroom culture medium as described in claim 1 containing walnut beta pruning sawdust, characterized in that include by weight with
Lower material component: walnut beta pruning sawdust 90, wheat bran 8, gypsum 2.
5. the Oyster mushroom culture medium as claimed in claim 2 containing walnut beta pruning sawdust, characterized in that the formula cultivation is put down
Mushroom A, biological transformation ratio is up to 117.75%, output increased 48.71%, and crude fiber content improves 16.67%, protein in fructification
Content improves 17.24%, polyoses content and improves 25%, fat content raising 66.67%, VC content raising 5.38%.
6. the Oyster mushroom culture medium as claimed in claim 2 containing walnut beta pruning sawdust, characterized in that the formula cultivation is put down
Mushroom A, biological transformation ratio is up to 90.92%, output increased 14.83%, and crude fiber content improves 33.33%, albumen in fructification
Matter content improves 25.86%, fat content and improves 66.67%, VC content raising 2.69%, total amino acid content raising 36.26%,
Wherein isoleucine, leucine and phenylalanine content are respectively increased 230%, 163.15% and 131.30%.
7. a kind of preparation method of the Oyster mushroom culture medium as claimed in claim 1 or 2 containing walnut beta pruning sawdust, it is characterized in that packet
Include following steps:
A. walnut beta pruning is ground into the particle that partial size is 3~8mm with pulverizer, is impregnated 12~24 hours with clear water, makes walnut
Beta pruning sawdust is sufficiently drenched；
B. first moisture extra in walnut beta pruning sawdust is leached, then adds respective substance to batch mixer by material proportion, mixes
Material machine stirs 20~30 minutes, and water content is added water in mixing process up to 60~80%；
C. mixed material is packed, sterilizes 2 hours at 121 DEG C, after sterilizing at 50~60 DEG C of the temperature of bacterium bag, ships it to and contain
In the low inoculation environment of bacterium amount, the preparation of oyster mushroom kind A cultivation matrix is completed, 12-28 hours to be cooled to 10~15 DEG C whens are fast
Quick access kind obtains oyster mushroom kind A after growth 33~38 days.
8. the preparation method of the Oyster mushroom culture medium as claimed in claim 7 containing walnut beta pruning sawdust, characterized in that the step
In rapid c, mixed material interruption is sterilized twice in summer, every time sterilizing 2 hours.
9. the preparation method of the Oyster mushroom culture medium as claimed in claim 7 containing walnut beta pruning sawdust, it is characterized in that include with
Lower specific steps:
Walnut beta pruning is ground into the particle that partial size is 5mm with pulverizer, is impregnated 18 hours with clear water, fills walnut beta pruning sawdust
Divide drenched；Water is drained every other day, respective substance is added to batch mixer by material proportion, stirs 20~30 minutes, mix in batch mixer
It closes when adding water to water content in whipping process up to 65~70%, pack sterilizes 2~4 hours at 121 DEG C, after sterilizing, in bacterium bag
It at 50~60 DEG C of temperature, ships it in the low inoculation environment of bacteria containing amount, the preparation of oyster mushroom kind A cultivation matrix is completed, to cold
But 12-28 hours to 10~15 DEG C when be quickly inoculated with；Oyster mushroom kind A is obtained after growth 33~38 days；
The material proportion includes: walnut beta pruning sawdust 63, cotton seed hull 30, wheat bran 5, gypsum 2 by weight.
10. the preparation method of the Oyster mushroom culture medium as claimed in claim 9 containing walnut beta pruning sawdust, characterized in that described
Material proportion is by weight are as follows: walnut beta pruning sawdust 90, wheat bran 8, gypsum 2.
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| CN114766283A (en) * | 2022-04-27 | 2022-07-22 | 贵州大学 | Auricularia polytricha culture medium containing walnut sawdust and preparation method thereof |
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