CN110283654A - A kind of anti-oxidant and relax bowel and defecation dual-purpose of drug and food tea oil preparation method - Google Patents
A kind of anti-oxidant and relax bowel and defecation dual-purpose of drug and food tea oil preparation method Download PDFInfo
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Abstract
The invention belongs to the preparation technical fields of dual-purpose of drug and food tea oil.The invention discloses a kind of anti-oxidant and relax bowel and defecation dual-purpose of drug and food tea oil preparation methods, comprising: drying, squeezing, degumming, decolorization process.Wherein squeezing is directly squeezed after being crushed using tea seed, and pressing temperature is mild, does not destroy its pharmacological activity；Using heating aquation degumming and the process for refining of heating of Activated carbon decoloring, Conditions Temperature is mild, is sufficiently reserved tea oil pharmacological component；The physicochemical property of gained tea oil meets 2015 editions " Chinese Pharmacopoeia " requirements simultaneously, can be used as medicinal tea oil.As from the chemical component of tea oil obtained by different preparation methods, physicochemical property detection, anti-oxidant and functions of loosening bowel relieving constipation comparison, show tea oil preparation method mild condition of the invention, simple process, can operate by force, gained tea oil is anti-oxidant and relax bowel and defecation activity is preferable, reach " Chinese Pharmacopoeia " requirement, it can be served, also pharmaceutically acceptable.The use scope for having expanded tea oil improves the economic value added of tea oil.
Description
Technical field
The present invention relates to the preparation methods of tea oil, and in particular to a kind of anti-oxidant and relax bowel and defecation dual-purpose of drug and food tea oil system
Preparation Method.
Background technique
Tea oil is plant of theaceae oil tea Camellia oleifera Abel or Camellia yuansienensis Camellia
The fat oil that the mature seed of meiocarpa Hu ms. is obtained with squeezing method.Tea oil mainly originates in Asia, and global tea oil is big
Part is come to China, wherein main growing area is in Hunan, Guizhou, zhejiang and other places.Content of fatty acid, ratio and the olive of tea oil
Oil is very much like, is known as the title of " east olive oil ", and the quality of tea oil has had reached " omega meals " international camp
The standard of supporting, is a kind of good edible oil.Tea oil is recorded in 2015 editions " Chinese Pharmacopoeias " (one), as injection tea oil simultaneously
Raw material and ointment bases.Modern pharmacological studies have shown that tea oil has anti-oxidant, antibacterial, anticancer, anti-inflammatory, liver protection, prevention coronary disease
The pharmacotoxicological effects such as disease and artery sclerosis and increase gastrointestinal tract absorption.Be used clinically for treatment radiotherapy after canker sore, scald
Wound, soft tissue bruise, crissum friction erythema, pressure sore and prevention Changes of Radiation Damaged Skin.
Currently, it is more about the preparation method of tea oil and the pharmacology activity research of tea oil, but combine the tea of its pharmacological activity
Oil making technology research is less.
Summary of the invention
Technical problem to be solved by the invention is to provide a kind of preparation methods of dual-purpose of drug and food tea oil, it can expand tea oil
Use scope, be suitable for tea oil exploitation into anti-oxidant food relevant with relax bowel and defecation, health care product and medicinal.
The present invention is implemented as follows: the preparation method of dual-purpose of drug and food tea oil, comprises the technical steps that:
1) dry shelling: camellia oleifera fruit is dried, is shelled, makes tea seed moisture content 5%-8%, obtains dry oil
Tea seed is spare；
(2) crude oil squeezes: dry tea seed being crushed to 20-30 mesh, investment squeezer is squeezed, then will squeezing
Liquid is filtered after standing 10min-20min precipitating, obtains tea oil crude oil；
(3) degumming: by tea oil heating crude oil to 30 DEG C -50 DEG C, 30 DEG C of -50 DEG C of distilled water are added in 4:1-1:1 by volume,
20min-30min is stirred, 10min-30min is then centrifuged for, takes supernatant, obtain degumming tea oil；
(4) it decolourizes: active carbon is added in degumming tea oil 2:1-1:2 in mass ratio, is heated to 40 DEG C -60 DEG C, is stood
20min-30min is then cooled to room temperature and is filtered, and obtains dual-purpose of drug and food tea oil.
Drying described in step 1) is that the sun dries or low temperature drying, temperature are lower than 60 DEG C.
Squeezing described in step (2) is cold-press, is squeezed at a temperature of 20 DEG C -60 DEG C after tea seed is crushed.
Dual-purpose of drug and food tea oil is preparing the application in anti-oxidation medicine.
The dual-purpose of drug and food tea oil meets 2015 editions " Chinese Pharmacopoeia " physicochemical property requirements, can be directly medicinal, can conduct
The raw material or ointment bases of injection tea oil.
Application of the dual-purpose of drug and food tea oil that the preparation method of tea oil obtains in preparation relax bowel and defecation drug.
The dual-purpose of drug and food tea oil meets 2015 editions " Chinese Pharmacopoeia " physicochemical property requirements, can be directly medicinal, can conduct
The raw material or ointment bases of injection tea oil.
In order to further verify technical effect of the invention, following experiment has been carried out:
1, the preparation of Different Preparation tea oil crude oil sample
1. cold-press: the camellia oleifera fruit sun dries, and shelling makes tea seed moisture content 5%, is crushed to 20 mesh, investment squeezing
Machine squeezes at 20 DEG C, stands 10min precipitating, rear to filter, and obtains cold press tea oil crude oil sample；
2. hot moulding method: the camellia oleifera fruit sun dries, and shelling makes tea seed moisture content 5%, is crushed to 20 mesh, steaming
Time is 40min, after cooling room temperature, is squeezed at 20 DEG C, and 10min precipitating is stood, rear to filter, and obtains hot moulding tea oil crude oil sample.
3. petroleum ether solvent extraction: the camellia oleifera fruit sun dries, and shelling makes tea seed moisture content 5%, is crushed to 20
Petroleum ether is added in mesh, and material ratio (w/v) 1:7 is extracted 3 times, extracted 2h every time, filter after taking-up, is concentrated under reduced pressure and removes solvent, so
It is removed water afterwards using appropriate anhydrous sodium sulfate, it is cooling, obtain petroleum ether extraction tea oil crude oil sample.
4. n-hexane solvent extraction method: the camellia oleifera fruit sun dries, and shelling makes tea seed moisture content 5%, is crushed to 20
N-hexane is added in mesh, and material ratio (w/v) 1:7 is extracted 3 times, extracted 2h every time, filter after taking-up, is concentrated under reduced pressure and removes solvent, so
It is removed water afterwards using appropriate anhydrous sodium sulfate, it is cooling, obtain n-hexane extraction tea oil crude oil sample.
2, the recovery rate of Different Preparation tea oil crude oil
As shown in Table 1, Different Preparation has a significant impact to tea oil crude oil recovery rate.Wherein, compared with cold-press,
The recovery rate of other 3 kinds of preparation processes all has significant difference.The recovery rate conspicuousness of hot moulding method be higher than cold-press (p <
0.01), there was no significant difference with n-hexane extraction for the recovery rate of petroleum ether extraction.Generally speaking, molten compared with squeezing method
The recovery rate of agent extraction is higher (p < 0.01).
The recovery rate (%) of the different extraction process tea oil crude oil of table 1
Note: compared with cold-press, p* < 0.05, p** < 0.01；Compared with petroleum ether extraction, p △ < 0.05, p △ △ <
0.01
3, Different Preparation tea oil crude oil physicochemical property detects
Referring to 2015 editions " Chinese Pharmacopoeia ", relative density, the index of refraction, acid value, saponification of Different Preparation tea oil are measured
Value and iodine number.It the results are shown in Table 2 and Fig. 1.4 grow tea oil samples physicochemical property difference it is unobvious.It is advised with 2015 editions " Chinese Pharmacopoeia "
Fixed range is compared, and the tea oil saponification number of cold press, hot moulding and petroleum ether extraction is relatively low, and the tea oil relative density of n-hexane extraction is inclined
Height, and iodine number is bigger than normal.
2 different process of table prepares the physicochemical property of tea oil crude oil
4, the GC-MS analysis of Different Preparation tea oil crude oil
Esterification is carried out to Different Preparation tea oil sample referring to standard GB/T/T 17376-2008, then sample introduction
GC-MS analysis is carried out, the results are shown in Table 3 and Fig. 2.The fatty acid that Different Preparation tea oil detects shares 16 kinds, wherein petroleum
Ether extraction detects 15 kinds of fatty acid, does not detect erucic acid.4 grow tea unsaturated fatty acid content total in oil samples 89%
(89.145%~89.815%) above, mainly have oleic acid (80.710%~81.265%), palmitinic acid (7.810%~
9.058%), linoleic acid (6.348%~6.875%).Content of fatty acid no significant difference in Different Preparation tea oil.
3 different process of table prepares the content of fatty acid (unit: %) of tea oil crude oil
5, the polyphenol content measurement of Different Preparation tea oil crude oil
The detection of polyphenol is referring to LS/T6119-2017 in tea oil sample.With absorbance to the concentration of standard items gallic acid
Make standard curve, as a result as shown in figure 3, fit curve equation formula are as follows: Y=0.0148X+0.0096, R=0.9996.According to tea
The absorbance of oil samples is converted into the concentration of gallic acid, the polyphenol content that different process prepares tea oil is calculated, as a result such as 4 institute of table
Show.As shown in table 4, different process has significant impact to polyphenol content in tea oil, and the polyphenol of sample contains in petroleum ether extraction
It measures at most (10.74 ± 1.95mg/kg), cold press sample takes second place (8.63 ± 1.29mg/kg), followed by n-hexane extraction oil
(7.40 ± 1.25mg/kg), the polyphenol content in hot moulding method oil sample are minimum (7.77 ± 0.25mg/kg).In squeezing method, cold-press
Higher than hot moulding method；In solvent extraction method, the effect of petroleum ether extraction is better than n-hexane extraction, and has extremely significant sex differernce.
Polyphenol content in 4 Different Preparation tea oil crude oil of table
Note: compared with petroleum ether extraction, p* < 0.05, p** < 0.01
6, the anti-oxidant comparative analysis of different process tea oil crude oil
Using DPPH and ABTS method, the antioxidant activity that different process prepares tea oil is investigated by positive control of Trolox,
Tea oil is prepared with different process respectively, linear analysis is done to DPPH and ABTS free radical scavenging activity and sample concentration, calculates IC50Value
With Trolox equivalent.IC of the Trolox to DPPH and ABTS clearance rate50Respectively 2.42 μ g/mL, 5.57 μ g/mL.As a result see Fig. 4
With table 5.Different Preparation tea oil all has antioxidation, and dose dependent is presented.Wherein cold press tea oil sample is free
Base Scavenging activity is most strong.
Anti-oxidant comparative analysis in 5 Different Preparation tea oil of table
7, the functions of loosening bowel relieving constipation comparative analysis of different process tea oil sample crude oil
Establish functional consitipation mouse model using loperamide hydrochloride, compare distinct methods extract tea oil to it is functional just
Secret mouse ink progradation, the influence for arranging defecation grain number in melena time and 5h for the first time.
Influence of the 7.1 Different Preparation tea oil to defecation grain number in mice with constipation for the first time defecation time and 5h
After taking kunming mice 150, adaptable fed 5 days, it is randomly divided into 15 groups, every group 10.That is model group, blank
Group, cold press/hot moulding/petroleum ether extraction/n-hexane extract basic, normal, high dosage (2,4,6mL/kg) group, Maren wan (2g/kg) group.
Administration group distinguishes the tea oil of the basic, normal, high dosage of orally administration, and blank group and model group give distilled water, and positive controls are given
The Maren wan of 2g/kg, once a day, continuous 7d, during which free diet, records the weight of each group mouse daily.After the last administration,
Each group mouse fasting 16h (drinking-water is unlimited), the administration group mouse oral of model group, positive controls and three dosage gives dosage
For the loperamide hydrochloride of 10mg/kg, naive mice gives the distilled water of same volume.Give loperamide hydrochloride 30min
Afterwards, the administration group mouse of positive controls and high, medium and low 3 dosage gives the prepared Chinese ink containing corresponding tested material, blank group and model
Group mouse gives prepared Chinese ink, starts simultaneously at timing.Every mouse is individually raised, during which free water, ingest, observe and record every small
Defecation grain number in mouse's head time row's melena required time, 5h.
Influence of the Different Preparation tea oil to the defecation grain number in mouse for the first time defecation time and 5h is as shown in table 6.By
Table 6 it is found that model group compared to the blank group, the time required to arranging melena for the first time and in 5h defecation grain number there are extremely significant sex differernce,
Show model foundation success (p < 0.01).Have the time required to the melena of row for the first time of different process tea oil high dose group mouse significant
Sex differernce (p < 0.05 and p < 0.01).Wherein compared with model group, the row for the first time of cold press group low, middle and high dose groups mouse is black
Toilet, which takes time, has extremely significant difference (p < 0.01) with defecation grain number in 5h, and hot moulding group only has middle and high dosage group to have pole
Significant difference (p < 0.01), petroleum ether extraction group only have high dose group the melena time of row for the first time (p < 0.01) and in (p <
0.05), defecation grain number has significant difference in high dose (p < 0.01) group 5h, and n-hexane extraction group only has high dose group and mould
There are significant differences for type group.The result shows that Different Preparation tea oil to arrange for the first time defecation grain number in melena time and 5h have it is aobvious
Work property influences, but 3 dosage groups all have extremely significant influence in cold press group.
Influence of the 6 Different Preparation tea oil of table to each defecation index of mouse
Note: with model group ratio, * p < 0.05, * * p < 0.01
Influence of the 7.2 different process tea oil to mice with constipation small intestine push
Administration group distinguishes the tea oil of the basic, normal, high dosage of orally administration, and blank group and model group give distilled water, positive right
The Maren wan of 2g/kg is given according to group, 1 time a day, continuous 7d, during which free diet, take the photograph water, daily record each group mouse weight become
Change situation.After the last administration, groups of animals fasting 16h (drinking-water is unlimited), model group, positive controls and different tested materials 3
The mouse oral of dosage administration group gives the loperamide hydrochloride that dosage is 10mg/kg, and naive mice gives the steaming of same volume
Distilled water.After giving loperamide hydrochloride 30min, positive controls and administration group mouse give the prepared Chinese ink containing corresponding tested material, empty
White group and model group mouse give prepared Chinese ink.The animal of small bowel peristalsis experiment is carried out after stomach-filling prepared Chinese ink 30min immediately at cervical dislocation
Extremely, it opens abdominal cavity and separates mesenterium, clip upper end is placed on pallet from the intestinal tube of pylorus, lower end to ileocecus, gently by small intestine
Straight line is pulled into, measurement small intestinal length is " total small intestinal length ", is from pylorus to prepared Chinese ink forward position " prepared Chinese ink propulsion length ".Based on formula
Calculate ink progradation: ink progradation (%)=prepared Chinese ink promotes length (cm)/total small intestinal length (cm) × 100%.Difference is extracted
The results are shown in Table 7 to mice with constipation small intestine push for method tea oil.
Compared with blank and positive controls, ink progradation significantly reduces (p < 0.01) in model group, illustrates to construct mould
Type success.Compared with model group, cold press group low (p < 0.05), in (p < 0.01), high dose group (p < 0.01) ink progradation it is equal
There are significant differences, and as the increase ink progradation of tea oil dosage is also higher；The middle and high dosage group ink progradation of hot moulding group
There are extremely significant difference (p < 0.01)；Petroleum ether extraction group middle dose group there were significant differences and high dose there are extremely significant difference (p
<0.01)；And there are significant difference (p < 0.05) for n-hexane extraction group high dose group.
Influence of 7 tea oil of table to mouse ink progradation
Note: with model group ratio, * p < 0.05, * * p < 0.01.
Arrange time of melena for the first time in conjunction with mouse, in 5h defecation grain number and ink progradation it is found that 3 dosage in cold press group
Have a significant impact to each index, and hot moulding tea oil, petroleum ether extraction, n-hexane extraction only in and/or high dose when
Time just shows significant impact.The result shows that: Different Preparation tea oil has certain functions of loosening bowel relieving constipation, cold press tea oil
Functions of loosening bowel relieving constipation it is best, it is finally n-hexane extraction that followed by hot moulding tea oil, which is petroleum ether extraction later,.
8, the purification of cold press tea oil crude oil
By to tea oil obtained by cold-press, hot moulding method, petroleum ether extraction and n-hexane extraction recovery rate, chemistry at
Point, physicochemical property, polyphenol content and pharmacological activity is anti-oxidant and functions of loosening bowel relieving constipation comparative analysis shows different preparation methods
The content of fatty acid gap of tea oil is little, although the recovery rate of cold-press is lower, cold-press polyphenol content is higher, it is anti-oxidant and
Functions of loosening bowel relieving constipation is best, therefore selection cold-press tea oil carries out the purification of lower step.Show referring to 2015 editions " Chinese Pharmacopoeias " cold
It is relatively low to squeeze tea oil saponification number, with this by aquation degumming and decoloration, improves saponification number content.
Specific step is as follows: 1. degumming: tea oil being heated to 30 DEG C, 30 DEG C of distilled water, stirring is added in 4:1 by volume
20min is centrifuged 10min afterwards, takes supernatant, obtains degumming tea oil；2. decoloration: activity is added in degumming tea oil 2:1 in mass ratio
Charcoal is heated to 40 DEG C, stands 20min, then cools to room temperature, and filters, and obtains purification tea oil.
The measurement of 8.1 purification tea oil physicochemical properties
Referring to 2015 editions " Chinese Pharmacopoeia ", relative density, index of refraction, acid value, saponification number and the iodine number of tea oil are measured.As a result
It is shown in Table 8 and Fig. 5.Tea oil color after purification is faint yellow, and physicochemical property is within the scope of States Pharmacopoeia specifications.
8 different process of table prepares the physicochemical property of tea oil
The anti-oxidant and functions of loosening bowel relieving constipation of 8.2 purification front and back tea oil
The antioxidant activity that purification front and back tea oil is measured using DPPH and ABTS method, establishes function using loperamide hydrochloride
Property mice with constipation model, measurement purification front and back tea oil to functional consitipation mouse ink progradation, for the first time row the melena time with
And in 5h defecation grain number influence.The results are shown in Table 9, refine front and back anti-oxidant there are no significant compared with functions of loosening bowel relieving constipation
It is not significant to show that aquation degumming and decoloration influence its pharmacological activity for difference (p > 0.05), this refining methd can retain tea oil
Anti-oxidant pharmacological activity chemical component relevant to relax bowel and defecation.
The anti-oxidant and functions of loosening bowel relieving constipation of the purification of table 9 front and back tea oil
The present invention is by chemical component, physicochemical property, polyphenol content and the pharmacological activity phase of the preparation method of tea oil and tea oil
In conjunction with, filter out optimal preparation method, wherein squeezing using tea seed crush after directly squeeze, pressing temperature is mild, does not break
Its bad pharmacological activity；Using heating aquation degumming and the process for refining of heating of Activated carbon decoloring, Conditions Temperature is mild, is sufficiently reserved
Tea oil pharmacological component；The physicochemical property of the dual-purpose of drug and food tea oil prepared is set to meet 2015 editions " Chinese Pharmacopoeia " requirements,
It can be used as medicinal tea oil, have the effect of anti-oxidant and relax bowel and defecation.As from tea oil obtained by different preparation methods chemistry at
Point, physicochemical property detection, anti-oxidant and functions of loosening bowel relieving constipation comparison, show tea oil preparation method mild condition of the invention,
Simple process can operate by force, and gained tea oil is anti-oxidant and relax bowel and defecation activity is preferable, reach " Chinese Pharmacopoeia " requirement, i.e., edible
With also pharmaceutically acceptable.The use scope for having expanded tea oil improves the economic value added of tea oil.
Detailed description of the invention
Fig. 1 is that Different Preparation of the invention prepares tea oil crude oil sample；
Fig. 2 is Different Preparation tea oil crude oil sample GC-MS total ion chromatogram of the invention；
Fig. 3 is gallic acid standard curve of the invention；
Fig. 4 is the oxidation resistance of Different Preparation tea oil crude oil of the invention；
Fig. 5 is the tea oil on (left side) and (right side) after purification before purification of the invention.
Specific embodiment
Embodiment 1: a kind of anti-oxidant and relax bowel and defecation dual-purpose of drug and food tea oil preparation method comprises the technical steps that:
(1) dry shelling: the camellia oleifera fruit sun being dried, shells, makes tea seed moisture content 5%, spare；
(2) crude oil squeezes: tea seed being crushed to 20 mesh, squeezer is put into, squeezes at a temperature of 20 DEG C, stands 10min
Precipitating, it is rear to filter, obtain tea oil sample；
(3) degumming: being heated to 30 DEG C for tea oil, and 30 DEG C of distilled water is added in 4:1 by volume, stirs 20min, rear to be centrifuged
10min takes supernatant, obtains degumming tea oil.
(4) it decolourizes: active carbon is added in degumming tea oil 2:1 in mass ratio, is heated to 40 DEG C, 20min is stood, then cools down
It to room temperature, filters, obtains a kind of anti-oxidant and relax bowel and defecation dual-purpose of drug and food tea oil.
Embodiment 2: a kind of anti-oxidant and relax bowel and defecation dual-purpose of drug and food tea oil preparation method comprises the technical steps that:
(1) dry shelling: by camellia oleifera fruit low temperature drying (temperature 60 C), shelling makes tea seed moisture content 8%, spare；
(2) crude oil squeezes: tea seed being crushed to 30 mesh, squeezer is put into, squeezes at a temperature of 60 DEG C, stands 20min
Precipitating, it is rear to filter, obtain tea oil sample；
(3) degumming: being heated to 50 DEG C for tea oil, and 40 DEG C of distilled water is added in 1:1 by volume, stirs 30min, rear to be centrifuged
30min takes supernatant, obtains degumming tea oil.
(4) it decolourizes: active carbon is added in degumming tea oil 1:2 in mass ratio, is heated to 60 DEG C, 30min is stood, then cools down
It to room temperature, filters, obtains a kind of anti-oxidant and relax bowel and defecation dual-purpose of drug and food tea oil.
Embodiment 3: a kind of anti-oxidant and relax bowel and defecation dual-purpose of drug and food tea oil preparation method comprises the technical steps that:
(1) dry shelling: the camellia oleifera fruit sun being dried, shells, makes tea seed moisture content 6%, spare；
(2) crude oil squeezes: tea seed being crushed to 20 mesh, squeezer is put into, squeezes at a temperature of 40 DEG C, stands 15min
Precipitating, it is rear to filter, obtain tea oil sample；
(3) degumming: being heated to 40 DEG C for tea oil, and 40 DEG C of distilled water is added in 2:1 by volume, stirs 25min, rear to be centrifuged
15min takes supernatant, obtains degumming tea oil.
(4) it decolourizes: active carbon is added in degumming tea oil 1:1 in mass ratio, is heated to 50 DEG C, 25min is stood, then cools down
It to room temperature, filters, obtains a kind of anti-oxidant and relax bowel and defecation dual-purpose of drug and food tea oil.
Detection test
One, the measurement of tea oil physicochemical property obtained by embodiment
Referring to 2015 editions " Chinese Pharmacopoeia ", physicochemical property (relative density, refractive power of measurement above embodiments gained tea oil
Rate, acid value, saponification number and iodine number).It the results are shown in Table 10.
The physicochemical property of the different embodiment tea oil of table 10
Two, tea oil obtained by embodiment is anti-oxidant and functions of loosening bowel relieving constipation
The antioxidant activity that purification front and back tea oil is measured using DPPH and ABTS method, establishes function using loperamide hydrochloride
Property mice with constipation model, measurement purification front and back tea oil to functional consitipation mouse ink progradation, for the first time row the melena time with
And in 5h defecation grain number influence.It the results are shown in Table 11.
The physicochemical property of the different embodiment tea oil of table 11
By table 10 and table 11 the result shows that, the physicochemical property of different embodiment tea oil meets 2015 editions " Chinese Pharmacopoeias "
It is required that it is medicinal to show that gained tea oil is used equally for.The anti-oxidant and functions of loosening bowel relieving constipation of different embodiment tea oil and cold press simultaneously
Difference (p > 0.05) that crude oil compares that there are no significant shows that tea oil preparation method of the invention retains the anti-oxidant of tea oil and profit
The relevant pharmacological activity chemical component of intestines defaecation.Show that tea oil preparation method of the invention has preferably by comparing above
Anti-oxidant and relax bowel and defecation, while meeting the requirement of 2015 editions " Chinese Pharmacopoeia " physicochemical properties, be that a kind of anti-oxidant and ease constipation is logical
Just the preparation method of dual-purpose of drug and food tea oil.
Claims (7)
1. a kind of preparation method of dual-purpose of drug and food tea oil, which is characterized in that comprise the technical steps that:
1) dry shelling: camellia oleifera fruit is dried, is shelled, makes tea seed moisture content 5%-8%, obtains dry tea seed
It is spare；
(2) crude oil squeezes: dry tea seed being crushed to 20-30 mesh, investment squeezer is squeezed, then that pressed liquor is quiet
It is filtered after setting 10min-20min precipitating, obtains tea oil crude oil；
(3) degumming: by tea oil heating crude oil to 30 DEG C -50 DEG C, 30 DEG C of -50 DEG C of distilled water, stirring is added in 4:1-1:1 by volume
20min-30min is then centrifuged for 10min-30min, takes supernatant, obtains degumming tea oil；
(4) it decolourizes: active carbon is added in degumming tea oil 2:1-1:2 in mass ratio, is heated to 40 DEG C -60 DEG C, stands 20min-
30min is then cooled to room temperature and is filtered, and obtains dual-purpose of drug and food tea oil.
2. the preparation method of tea oil according to claim 1, it is characterised in that: drying described in step 1) is sun solarization
Dry or low temperature drying, temperature are lower than 60 DEG C.
3. the preparation method of tea oil according to claim 1 or 2, it is characterised in that: squeezing described in step (2) is cold press
Method is squeezed at a temperature of 20 DEG C -60 DEG C after crushing tea seed.
4. the dual-purpose of drug and food tea oil that a kind of preparation method of tea oil according to claim 3 obtains is preparing anti-oxidation medicine
In application.
5. application according to claim 4, it is characterised in that: the dual-purpose of drug and food tea oil meets 2015 editions " middle traditional Chinese medicines
Allusion quotation " physicochemical property requirement, can be directly medicinal, it can be as the raw material or ointment bases of injection tea oil.
6. the dual-purpose of drug and food tea oil that a kind of preparation method of tea oil according to claim 3 obtains is in preparation relax bowel and defecation medicine
Application in object.
7. application according to claim 6, it is characterised in that: the dual-purpose of drug and food tea oil meets 2015 editions " middle traditional Chinese medicines
Allusion quotation " physicochemical property requirement, can be directly medicinal, it can be as the raw material or ointment bases of injection tea oil.
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Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102757858A (en) * | 2012-07-26 | 2012-10-31 | 安徽亿宏生物科技有限公司 | Preparation method for tea oil by double helix low-temperature squeezing |
| CN103013651A (en) * | 2013-01-10 | 2013-04-03 | 南京财经大学 | Method for producing low-temperature cold-pressed and refined tea seed oil |
| CN103113979A (en) * | 2013-02-04 | 2013-05-22 | 华南农业大学 | Method for producing edible camellia oleosa seed oil |
| CN104789349A (en) * | 2015-03-31 | 2015-07-22 | 湖南大三湘油茶科技有限公司 | Super virgin camellia oil and preparation method thereof |
| CN105695080A (en) * | 2016-03-25 | 2016-06-22 | 贵州务川红宇生物科技开发有限公司 | Preparation method of tea oil |
| CN105985861A (en) * | 2016-06-06 | 2016-10-05 | 浙江省农业科学院 | Production method of natural-vitamin-E-rich oil tea seed oil |
| CN107142150A (en) * | 2017-04-01 | 2017-09-08 | 江西神州通油茶科技有限公司 | The preparation method and tea oil of originally-ecological cold pressing tea oil |
| CN108192725A (en) * | 2018-01-26 | 2018-06-22 | 何长庚 | A kind of processing technology of oil tea |
-
2019
- 2019-06-26 CN CN201910563797.9A patent/CN110283654A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102757858A (en) * | 2012-07-26 | 2012-10-31 | 安徽亿宏生物科技有限公司 | Preparation method for tea oil by double helix low-temperature squeezing |
| CN103013651A (en) * | 2013-01-10 | 2013-04-03 | 南京财经大学 | Method for producing low-temperature cold-pressed and refined tea seed oil |
| CN103113979A (en) * | 2013-02-04 | 2013-05-22 | 华南农业大学 | Method for producing edible camellia oleosa seed oil |
| CN104789349A (en) * | 2015-03-31 | 2015-07-22 | 湖南大三湘油茶科技有限公司 | Super virgin camellia oil and preparation method thereof |
| CN105695080A (en) * | 2016-03-25 | 2016-06-22 | 贵州务川红宇生物科技开发有限公司 | Preparation method of tea oil |
| CN105985861A (en) * | 2016-06-06 | 2016-10-05 | 浙江省农业科学院 | Production method of natural-vitamin-E-rich oil tea seed oil |
| CN107142150A (en) * | 2017-04-01 | 2017-09-08 | 江西神州通油茶科技有限公司 | The preparation method and tea oil of originally-ecological cold pressing tea oil |
| CN108192725A (en) * | 2018-01-26 | 2018-06-22 | 何长庚 | A kind of processing technology of oil tea |
Non-Patent Citations (3)
| Title |
|---|
| 关继华，等: "茶籽冷榨法制取茶油工艺研究!", 《应用化工》 * |
| 刘金勇，等: "注射用油茶籽油中杂质控制的研究现状", 《粮油食品科技》 * |
| 罗质: "《油脂精炼工艺学》", 30 June 2016, 中国轻工业出版社 * |
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Application publication date: 20190927 |