CN100486606C - Total sennoside extract for treating constipation and its extraction process - Google Patents
Total sennoside extract for treating constipation and its extraction process Download PDFInfo
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Abstract
Total sennoside extract for treating constipation and its extraction process are disclosed. The extraction process has simple operation, low cost and high total sennoside content, over 50 %, in the extract. The total sennoside extract may be prepared into different preparation forms, capsule, table, soft capsule, dripping pill, etc. The total sennoside extracting process includes the following steps: adding solvent to senna leaf as the medicinal material, heating reflux or soaking for 1-5 times of 10 min to 3 hr each, filtering to obtain filtrate, regulating pH value to 1-10, centrifugal treatment, water elution in macroporous resin column, depurating with dilute alcohol solution, elution with 21-95 % concentration alcohol solution, collecting eluted solution, concentration, and drying to obtain yellow brown powder as the extract.
Description
Technical field
The present invention relates to the extract of the effective site sennoside of treatment constipation in the Chinese medicine Folium Sennae, the multiple pharmaceutical preparation made from this extract belongs to field of medicaments.
Background technology
Folium Sennae is a Chinese medicine, be the dried leaves of narrow leaf Senna fruit of leguminous plant (Cassia angustifolia Vahl.) or cassia acutifolia Delile (C.acutifolia Lelile.), record in " first one of Chinese pharmacopoeia version in 2000 is one of purgatives medicine commonly used clinically, nature and flavor are sweet, bitter, cold, slightly poisonous, return liver, spleen, stomach and large intestine channel, can rush down long-pending heat, logical stool, the heat-clearing and toxic substances removing function of gallbladder promoting is controlled constipation with heat retention, the long-pending abdominal distention of rushing down.Be used for that accumulation of heat is stagnant, the treatment of constipation stomachache, edema distension.In recent years, it is prepared as before abdominal part and the various image checks of intestinal or operation front intestinal road cleaning by main both at home and abroad, cures mainly various constipation, particularly senile and obstipation, the abdominal post-operation intestinal function recovery is also had facilitation, is the most widely used catharsis agent in the whole world.The fruit of Folium Sennae also is used as catharsis agent Application and Development.Fitochemical studies shows that the effective ingredient of Folium Sennae diarrhea inducing is an anthra-glucoside, and its active component is Sennoside A, B, C, D etc.Sennoside A wherein, the discharge function of B is the strongest.Its diarrhea inducing composition sennoside is through stomach, little intestinal absorption, decomposes in liver, the operation of product menses and excited ganglia pelvina to be to shrink large intestine, to stimulate colon, and its wriggling is accelerated, and reaches discharge function.With Folium Sennae 2-6 gram, the bubble boiled water is drunk, except that the general constipation of treatment, to damage back abdominal distention, the constipation curative effect is better, symptom is obviously improved.To the operation of opening cranium constipation, longer with the Folium Sennae duration of efficacy, advance the motion of people's intestinal propelling property, large intestine is reduced the absorption of moisture, the patient feels that gastrointestinal peristalsis strengthens in the abdomen more, and is easily strong, and general needs medication 1-2 time. discover, the quiet notes sennoside of mice does not have discharge function, orally then 100% produces effect.When the various catabolites of Folium Sennae were directly injected caecum, the chrysophanic acid anthrone rushed down that activity is the strongest down, and consistent with the site of action of Folium Sennae, so one of sennoside metabolite in vivo chrysophanic acid anthrone is an active component under real the rushing down of sennoside.
At present, the Folium Sennae extraction of active ingredients adopts following method usually:
1. alcohol reflux extracts (or diafiltration) method: get dry Folium Sennae, add an amount of alcoholic solution, heating and refluxing extraction 2-3 time (or diafiltration extraction) is filtered, and filtrate decompression concentrates, and drying gets Folium Sennae extract.
2. decocting boils (or brewing) method: get dry Folium Sennae, add suitable quantity of water, reflux (or boiling water immersion) is extracted 1-5 time, filters, and filtrate decompression concentrates, and drying gets Folium Sennae extract.
No matter be to adopt alcohol extracting method or water extraction, do not contain a large amount of impurity owing to do further refinement treatment gained Folium Sennae extract, so total sennoside content is very low, high-load can only reach about 5%.The too high meeting of impurity level produces certain toxic and side effects to human body in the extract, also brings certain difficulty to formulation preparation simultaneously, makes the dosage of preparation be not easy control, and content of effective is also unstable.
In addition, because active constituent content is lower in the present Folium Sennae extract, for reaching the needed curative effect of treatment constipation, patient must take than the strong dose thing, and is very inconvenient.In addition, do not see the application of macroporous adsorbent resin in Folium Sennae is extracted so far as yet.
Summary of the invention
The technical problem to be solved in the present invention is medicine and the various oral formulations thereof that development contains Folium Sennae effective site total sennoside, the oral drugs of the present invention's development, and the content of effective height, impurity content is few, good effect.The extracting method of this total sennoside, not only easy and simple to handle, and reduced cost, the content of prepared total sennoside is higher.
The objective of the invention is to realize by following measure:
A kind of total sennoside extract for the treatment of constipation, the weight percentage that it is characterized in that active component total sennoside in this extract is in dry product 〉=50%, its extracting method is: get the Folium Sennae medical material, add solvent, adopting reflux or insulation to soak extracts 1-5 time, each 10min-3h, filter to get filtrate and regulate pH to 1-10, centrifugal treating, last macroporous adsorptive resins, water eluting impurity, (5%-20%) roguing of the low alcohol of reuse, 21-95% alcoholic solution eluting is collected ethanol elution, concentrate eluant, drying get pale brown toner end extract then.
In this extract the weight percentage of Sennoside A in dry product 〉=17%, sennoside B weight percentage in dry product 〉=33%.
But said extracted thing and medical carrier are or/and any preparation that excipient is made comprises capsule, tablet, soft capsule, drop pill or oral liquid.
Solvent for use is in the extracting method of the present invention: water, ethanol, methanol or acetone; Heating-up temperature is 40-100 ℃.The rotating speed of centrifugal treating is 200-20000 rev/min.The macroporous adsorptive resins model can be D101, DA201, D301 or D401 type.Weight percentage 〉=50% of active component total sennoside in the extract of the pale brown toner of this extracting method gained end.
The present invention has compared to existing technology as advantage:
Above-mentioned total sennoside preparation, its preparation of making meet the requirement of " medicine registration management way " Chinese medicine, natural drug registration classification 5, i.e. not the effective site and the preparation thereof that extract from materials such as plant, animal, mineral of list marketing at home.
1, total sennoside extract among the present invention with pharmaceutically acceptable carrier and/or mixed with excipients, is made and is contained the oral formulations that above-mentioned total sennoside is a main active, as capsule, tablet, soft capsule, drop pill, oral liquid etc.
Because mainly onset in gastrointestinal tract of total sennoside, therefore total sennoside is made oral formulations, every (sheet) contains the capsule, tablet, soft capsule, drop pill, oral liquid of total sennoside 5～150mg etc.Be generally the total sennoside that people's consumption per day is 5～150mg every day, preferred every (sheet) contains capsule, tablet, soft capsule, drop pill, oral liquid of the total sennoside of above-mentioned total sennoside 30mg etc.
2, the extracting method of total sennoside of the present invention, utilization macroporous adsorbent resin isolation technics, the method is not only easy and simple to handle, and has reduced cost, and the content of the sennoside of extraction must not be lower than 50%, and very is fit to suitability for industrialized production.Studies show that in a large number macroporous resins such as D101, DA201, D301, D401 are very good to the adsorbing separation effect of sennoside, improved the purity of extract greatly.
Folium Sennae extract contains a large amount of impurity in the prior art in order to overcome, total sennoside content is very low, the present invention has introduced macroporous adsorption resin technology serving as to extract on the basis of solvent extraction with aqueous solution and other solvent, has improved the content of total sennoside in the dry extract greatly.Not only reduced toxic and side effects, made things convenient for the preparation of preparation, made the dosage of preparation more controlled, active constituent content is more stable, and the dosage that patient takes medicine also significantly reduces, and has made things convenient for the patient to take, and has increased the compliance of patient to medicine.Can make dosage forms such as capsule, tablet, effervescent tablet, slow releasing agent or liposome, be used for the treatment of constipation.And do not see the application of macroporous adsorbent resin in Folium Sennae is extracted so far as yet.
The total sennoside content that the present invention extracts is at least 50%, and its main component is a total sennoside, and by high performance liquid chromatography, main component is sennoside A and sennoside B.
Chromatographic condition and system suitability test
ZORBEX SB-C18 post, with oxolane: 1.75% acetic acid (15:85) carries out gradient elution; The detection wavelength is 270nm.
| Time min | A (oxolane) % | B (acetic acid) % |
| 0 | 15 | 85 |
| 4.9 | 15 | 85 |
| 5.0 | 13 | 87 |
| 40 | 13 | 87 |
The preparation of reference substance solution
Precision takes by weighing Sennoside A, B reference substance 1.0mg respectively, places the 10ml measuring bottle respectively, with 50% ethanol ultrasonic dissolution, gets Sennoside A reference substance liquid chromatogram, gets sennoside B reference substance liquid chromatogram.Be analyzed as follows by the FXY liquid phase process: Sennoside A reference substance liquid chromatogram as shown in Figure 1.Sennoside B reference substance liquid chromatogram, as shown in Figure 2.
The preparation of need testing solution
Precision takes by weighing total sennoside extract powder 0.2g of the present invention, the accurate 50% ethanol 25ml that adds, and ultrasonic 30 minutes, put coldly, supply weight with 50% ethanol, filtration, promptly.By the FXY liquid phase process analyze Fig. 3.
Description of drawings
Fig. 1 is a Sennoside A reference substance liquid chromatogram.
Fig. 2 is a sennoside B reference substance liquid chromatogram.
Fig. 3 is the liquid chromatogram of test sample total sennoside intermediate of the present invention.
The specific embodiment
The following example is intended to further describe for example the present invention, but is not to limit the present invention by any way.
The preparation method of Folium Sennae extract provided by the invention may further comprise the steps:
Get the Folium Sennae medical material, pulverize or whole leaf, add solvent, reflux or insulation are soaked and are extracted, and regulate pH to 1-10, centrifugal treating, concentrate (extraction with aqueous solution can not concentrate) last macroporous adsorptive resins, first water eluting impurity, reuse hangs down pure roguing, 21-95% alcoholic solution eluting is collected ethanol elution, concentrate eluant, drying gets pale brown toner end.
Embodiment one
Get Folium Sennae medical material (India's product) 10kg, pulverize, extract three times with 100L, 80L, 80L boiling water solution soaking respectively, each 30min merges three times filtrate, regulates pH to 3-4, centrifugal, last D301 macroporous adsorptive resins, first water are eluted to the Molish reaction and are negative, reuse 20% low pure roguing, 40% ethanol elution is collected ethanol elution, concentrate eluant, drying gets pale brown toner end.The HPLC method is measured, and sennoside content is 52%.
Embodiment two
Get Folium Sennae medical material (India's product) 50kg, respectively with the alcoholic solution backflow lixiviate of 1000L, 800L, 800L 70% three times, each 60min, merge three times filtrate, being concentrated into does not have alcohol, regulates pH to 7-10, centrifugal, last D401 macroporous adsorptive resins, first water are eluted to the Molish reaction and are negative, reuse 20% low pure roguing, 70% ethanol elution is collected ethanol elution, concentrate eluant, drying gets pale brown toner end.The HPLC method is measured, and sennoside content is 85%.
Embodiment three
Getting Folium Sennae medical material (India's product) 100kg, pulverize, is 80% acetone soln reflux, extract, three times with 1000L, 800L, 800L concentration respectively, each 45min merges three times filtrate, is concentrated into not contain acetone, regulate pH to 4, centrifugal, last D201 macroporous adsorptive resins, elder generation's water is eluted to the Molish reaction and is negative, reuse 20% low pure roguing, 50% ethanol elution, collect ethanol elution, concentrate eluant, drying gets pale brown toner end.The HPLC method is measured, and sennoside content is 72%.
Embodiment four
Get Folium Sennae medical material (India's product) 100kg, pulverize, extract three times with the sodium carbonate boiling water solution soaking of 1000L, 800L, 800L1% concentration respectively, each 45min merges three times filtrate, regulates pH to 7, centrifugal, last D201 macroporous adsorptive resins, first water are eluted to the Molish reaction and are negative, reuse 15% low pure roguing, 50% ethanol elution is collected ethanol elution, concentrate eluant, drying gets pale brown toner end.The HPLC method is measured, and sennoside content is 65%.
Embodiment five
Get Folium Sennae medical material (India's product) 10kg, pulverize, extract three times with the sodium bicarbonate boiling water solution boiling water solution soaking of 100L, 80L, 80L10% concentration respectively, each 30min merges three times filtrate, regulates pH to 1, centrifugal, last D301 macroporous adsorptive resins, first water are eluted to the Molish reaction and are negative, reuse 25% low pure roguing, 35% ethanol elution is collected ethanol elution, concentrate eluant, drying gets pale brown toner end.The HPLC method is measured, and sennoside content is 60%.
Embodiment six
Get Folium Sennae medical material (India's product) 50kg, extract three times with 1000L, 800L, 800L sodium bisulfate boiling water solution soaking respectively, each 60min, merge three times filtrate, regulate pH to 8, centrifugal, last D401 macroporous adsorptive resins, elder generation's water is eluted to the Molish reaction and is negative, reuse 20% low pure roguing, 30% ethanol elution, collect ethanol elution, concentrate eluant, drying gets pale brown toner end.The HPLC method is measured, and sennoside content is 70%.
Embodiment seven
Get Folium Sennae medical material 50kg, add the 1000L methanol solution, heating and refluxing extraction once, reuse filters each time extracting solution with volume methanol extraction 1-2 time, and merging filtrate is concentrated into does not have alcohol, and thin up, regulate pH to 7-9, centrifugal, last D301 macroporous adsorptive resins, first water are eluted to the Molish reaction and are negative, reuse 15% low pure roguing, 70% ethanol elution is collected ethanol elution, concentrate eluant, drying gets pale brown toner end.The HPLC method is measured, and sennoside content is 91%.
Embodiment eight
The preparation of total sennoside sheet
Get embodiment one and extract the total sennoside raw material 35g that obtains, starch 50g, dextrin 50g, mixing is granulated, and sieves, and it is an amount of to add magnesium stearate after the drying, and tabletting gets 1000 (35mg/ sheets), promptly.Usage and dosage is: oral, and each 1～2, once a day.
Embodiment nine
Get embodiment one and extract the total sennoside raw material 33g that obtains, dextrin 102g, mixing is granulated, and sieves, and it is an amount of to add magnesium stearate after the drying, adorns 1000 capsules (35mg/ grain), promptly.Usage and dosage is: oral, and each 1～2, once a day.
Embodiment ten
Get embodiment two and extract the total sennoside raw material 35g that obtains, earlier it was pulverized 100 mesh sieves, mixes with 50ml Semen Maydis oil (or other plant oil, silicone oil, liquid paraffin etc.) again, grind evenly, be pressed into soft capsule, make altogether 1000 (35mg/ grains), promptly.Usage and dosage is: oral, and each 1～2, once a day.
Embodiment 11
Getting embodiment four and extract the total sennoside raw material 39.2g that obtains, be dissolved among the PEG6000 of 547.8g, is coolant with vegetable oil, and dropping preparation method makes 2000 of drop pill (18mg/ grain), promptly.Usage and dosage is: oral, and each 2～4, once a day.
Embodiment 12
Get embodiment six and extract the total sennoside raw material 28g that obtains, add simple syrup or aspartame or other correctivess,, be distributed into 1000, make oral liquid (35mg/ props up), promptly to 10L.Usage and dosage is: oral, and each 1, once a day.
The test of pesticide effectiveness of total sennoside of the present invention:
The total sennoside extract that the present invention is extracted has carried out following pharmacodynamics test:
A: to the influence of mouse small intestine propelling rate
Get 40 of Kunming mouses, body weight 22～26g, fasting 18h (freely drinking water) is divided into 4 groups at random, 10 every group.The administration component is that (the 100g Folium Sennae is soaked in the boiling water for five times Folium Sennae stock solution group, each 500ml, be concentrated into 100ml, total sennoside content about 1%), (embodiment 1 is extract obtained for the total sennoside group, wherein total sennoside content about 52%, as follows), irritate stomach Folium Sennae and total sennoside charcoal end suspension 0.2ml according to every 10g body weight, matched group is irritated stomach equivalent normal saline charcoal end suspension.30min after administration, cervical vertebra dislocation is put to death, and opens abdominal part, and the intercepting pylorus is measured respectively from sphincter of pylorus to charcoal end and is reached the caecum distance foremost to the caecum section, is the intestinal propulsion ratio with the two percentage ratio, obtains each class mean.
The total sennoside group is compared charcoal end propelling rate and is obviously accelerated (p＜0.05) with matched group as a result, and no significant difference sees Table 1 between Folium Sennae group and the matched group.
Table 1 sennoside is to the influence of mouse small intestine propulsion functions
Annotate:
*Compare with the normal saline group,
*P＜0.05
B. to the influence of isolated rat ileum autonomic movement
Get 24 of SD rats, fasting 24h (freely drinking water), the cervical vertebra dislocation is put to death, fetch intestinal segment 2.5cm, one end is fixed on the Z type ventilation hook, and the other end is connected with tension pick-up, and intestinal tube is moved in the Magnus' bath, (groove includes tyrode's solution 40ml, 37 ℃ of constant temperature), after waiting to stablize, trace normal intestinal tube motion earlier in contrast by physiograph, add normal saline 0.4ml more respectively, total sennoside group 0.4ml each 1 time before each administration, all washes intestinal tube 3 times with nutritional solution, instrument to be recorded show recover normal after, medicine-feeding test again.Observe before and after the administration variation of intestine in vitro wriggling amplitude and frequency.
The result shows: with administration metagaster wriggling amplitude significant difference (p＜0.05) is arranged relatively before the administration of sennoside group; Relatively there is not significant difference before the matched group administration with after the administration.Two groups of ileums wriggling amplitude and frequency there is no significant change and see Table 2.
Table 2. sennoside is to the shadow noon of isolated rat ileum wriggling amplitude and frequency
Annotate:
*With compare p＜0.05 before the administration
C. big intestinal contents transmission time of rat
Female Wistar rats, body weight 200～250g, etherization, in caecum, distal end of catheter is fixed on the animal cervical region with a PVC conduit heeling-in.Recover in the rearging cage of single cage raising in back and band metal grill feces to be dropped on observing with paper.Give Folium Sennae and total sennoside 10mg, 10 animals of each dosage group are injected carmine (Carmine red, every animal 10mg is dissolved in the 0.4ml distilled water) from conduit immediately, and matched group is irritated stomach equivalent normal saline charcoal.The time that coloured feces appears in record first sees Table 3.
Table 3 sennoside is to the big intestinal contents of the rat transmission time
Annotate:
*With compare p＜0.05 before the administration
D: to the influence of urine amount
Adopt the skeleton symbol fecaluria to separate the metabolic cage method, get 45 of SD rats, 218～223g, male and female half and half, fasting (freely drinking water) 12h is divided into Folium Sennae preserved material stock solution group, 25% Folium Sennae preserved material group and normal saline group.Gently press the thing abdominal part before the test, get rid of surplus urine, load as water with every Mus 1 department's normal saline lumbar injection, the administration group gavages Folium Sennae group, Folium Sennae total glycosides group respectively, matched group equivalent physiologic saline for substitute medicinal liquid, 3 in every cage.Collect 0.5,1,2 respectively, 4h urine amount.As 10%, calculate the ratio of administration group with the urine amount of matched group.The result shows: Folium Sennae is organized 0.5,1, and the urine amount increases by 10.2%, 11.3% and 28.3% than matched group respectively during 4h, reduces 25.3% than matched group during 6h; The total sennoside group is 0.5, and 1h compares respectively during 4h according to group increase by 16.9%, 17.7% and 21.5%, reduces 41.7% than matched group during 6h, sees Table 4.
Table 4 sennoside is to the influence (n=15) of rat urine amount
Annotate: compare with normal saline, (+) increases percentage rate, and (-) reduces percentage rate
Raw material-Folium Sennae used in the present invention meets " relevant regulation under Folium Sennae item of Chinese pharmacopoeia version in 2000.
Claims (7)
1, a kind of total sennoside extract for the treatment of constipation, the weight percentage that it is characterized in that active component total sennoside in this extract is in dry product 〉=50%, its extracting method is as follows: get the Folium Sennae medical material, add solvent, adopting reflux or insulation to soak extracts 1-5 time, each 10min-3h, filter to get filtrate and regulate pH to 1-10, centrifugal treating, last macroporous adsorptive resins, water eluting impurity, reuse hangs down pure roguing, and 21-95% alcoholic solution eluting is collected ethanol elution, concentrate eluant, drying get pale brown toner end extract then.
2, the total sennoside extract of treatment constipation according to claim 1, the weight percentage that it is characterized in that Sennoside A in this extract in dry product 〉=17%, sennoside B weight percentage in dry product 〉=33%.
3, the total sennoside extract of treatment constipation according to claim 1, but it is characterized in that this extract and medical carrier are or/and any preparation that excipient is made.
4, the total sennoside extract of treatment constipation according to claim 3 is characterized in that described preparation is capsule, tablet, drop pill or oral liquid.
5, the total sennoside extract of treatment constipation according to claim 1 is characterized in that solvent for use is in the extracting method: water, ethanol, methanol or acetone; The reflux temperature is 40-100 ℃.
6, the total sennoside extract of treatment constipation according to claim 1, the rotating speed that it is characterized in that centrifugal treating in the extracting method is 200-20000 rev/min.
7, the total sennoside extract of treatment constipation according to claim 1 is characterized in that the model of macroporous adsorptive resins described in the extracting method is D101, DA201, D301 or D401 type.
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| CN101209278B (en) * | 2006-12-27 | 2013-03-27 | 天津中新药业集团股份有限公司 | Folium sennae extract and preparation thereof |
| US20110033563A1 (en) * | 2009-08-05 | 2011-02-10 | Horacio Peraino | Stabilized Senna Extract Gel Formulation and Method of Preparation |
| CN109966337B (en) * | 2019-03-28 | 2021-07-20 | 湖南华诚生物资源股份有限公司 | Method for extracting sennoside from senna leaf |
| CN113861250A (en) * | 2021-11-11 | 2021-12-31 | 江苏艾迪药业股份有限公司 | A method for preparing high purity sennoside and sennoside or its derivatives |
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Assignee: Nanjing Hailing Pharmaceutical Co., Ltd. of Yangtze River Pharmaceutical Group Assignor: Nanjing Hailing Chinese Medicine Pharmaceutical Technology Research Co.,Ltd. Contract record no.: 2011320000102 Denomination of invention: Total sennoside extract for treating constipation and its extraction process Granted publication date: 20090513 License type: Exclusive License Open date: 20060802 Record date: 20110228 |
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