CN100441166C - Combination of medication of containing medicinal rhubarb and activated carbon or carbo medicinalis - Google Patents
Combination of medication of containing medicinal rhubarb and activated carbon or carbo medicinalis Download PDFInfo
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- CN100441166C CN100441166C CNB2005100019865A CN200510001986A CN100441166C CN 100441166 C CN100441166 C CN 100441166C CN B2005100019865 A CNB2005100019865 A CN B2005100019865A CN 200510001986 A CN200510001986 A CN 200510001986A CN 100441166 C CN100441166 C CN 100441166C
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Abstract
The present invention provides a medical composition comprising rhubarb and active carbon or medicinal carbon. The medicine of the present invention can be in the preparation forms of double layer for oral taking, external paste, external application or administration by the mucosa. The weight ratio of the rhubarb to the active carbon or medicinal carbon is from 1:1 to 100:1, and the rhubarb and the active carbon or medicinal carbon have a synergistic effect. The present invention also provides a production technology of the composition and an application of the composition in preparing medicines for the prevention, health care or treatment of inflammation and diseases of the digestive tract.
Description
Technical field
The present invention relates to a kind of pharmaceutical composition and health product that comprise Radix Et Rhizoma Rhei and activated carbon or medicinal charcoal, specifically relate to strengthen synergistic pharmaceutical composition of having of Radix Et Rhizoma Rhei beneficial effect and health product.
Background technology
Radix Et Rhizoma Rhei is that Radix Et Rhizoma Rhei is the dry root and rhizome of polygonum rheum palmatum Rheum Palmatum L., Rheum tanguticum Rheum tanguticum Maxim.ex Balf. or Rheum officinale Rheum officinaleBaill..Preceding two kinds of habits are called " Beijing University's Huang ", and a kind of habit in back is called " RADIX ET RHIZOMA RHEI OFFICILALIS ", are born in high mountain border or half dark and damp place, grass slope more.Autumn Mo in the withered or inferior spring of stem and leaf excavates before germinateing, and removes radicula, scrapes off crust, valve cutting or section, and rope is worn the dry or direct drying of bunchiness.
Root and the rhizome of congener Radix Rhei emodi R.emodi Wall., Rheum hotaoense C. Y. Cheng et C. T. Kao (rheum rhabarbarum) R.hotaoense C.Y.Cheng et C.T.Kao, North China Radix Et Rhizoma Rhei R.fnanzenbachiiM ü nt., Radix et Rhizoma Rhei Wittrockii R.Wittrochii Lundstr. etc. are in some areas or title Radix et Rhizoma Rhei Franzenbachii among the people or Radix Rumicis.These kinds are not genuine rhubarbs, though contain the anthraquinone derivative composition, do not contain the Senna fruit glycoside yet, so discharge function is very poor, generally make the raw material that industrial dyestuff was used or done to veterinary drug.
Radix Et Rhizoma Rhei bitter in the mouth cold in nature is returned spleen, stomach, large intestine, liver, pericardium channel.Have effects such as purging heat and dredging bowels, removing pathogenic heat from blood and toxic substance from the body, eliminating blood stasis and inducing menstruation, dampness removing jaundice eliminating, the external application removing toxic substances and promoting subsidence of swelling that relieves inflammation or internal heat.Its Main Ingredients and Appearance is anthraquinone class, stilbene glycoside, chromone class, phenol glycoside, tannin etc.
1 discharge function
Radix Et Rhizoma Rhei has discharge function, is used for the treatment of constipation due to dry stool, constipation with heat retention, and generally 6～19h can discharge loose stool after medication.The Main Ingredients and Appearance of its diarrhea inducing is an anthraquinone derivatives, and is wherein the strongest with the effect of Senna fruit glycoside, free type anthraquinone discharge function a little less than.
1.1 acute ileus: grind various intestinal obstruction 44 examples of treatment of taking after mixing it with water into powder with the single Radix Et Rhizoma Rhei, each 9g takes medicine 1～3 time every day 2 times, and patient gets final product aerofluxus, defecation in 4～24h, and abdominal distention stomachache is alleviated, restoration of gastrointestinal function, and effective percentage reaches 97.7%.
1.2 infantile malnutrition: with Radix Et Rhizoma Rhei compatibility Semen Persicae, Fructus Gardeniae, Mirabilitum, Semen Armeniacae Amarum, be decocted in water for oral dose, every day 2 times, effect is better.
1.3 various acute pesticide intoxications: except that adopting the conventional emergency measures of western modern medicine, but also decocting Radix Et Rhizoma Rhei, Natrii Sulfas, each 20g of Radix Glycyrrhizae, or directly boiled water brews the single Radix Et Rhizoma Rhei, extracting juice 500ml, in gastric lavage after stomach tube pour into, if gone the oral or nasal feeding of stomach tube graded.Behind patient's defecation, be taken after being infused in hot water or decoction with above-mentioned 3 kinds of each 10g of medicine every day, and 17 examples are treated in logotype 2 days altogether, all are cured.
2 antipyretic effects
Radix Et Rhizoma Rhei has the clearing away heat-fire effect, and its mechanism of action is: Radix Et Rhizoma Rhei can reduce PGE content in the infected rabbits ventriculus tertius perfusate, influences the level of backbone ring nucleoside phenol, thereby reaches cooling effect.
2.1 acute pancreatitis hyperpyrexia: Radix Et Rhizoma Rhei 30～60g, add boiled water 120～200ml, soak 15～30min, divides 3 times oral, treat the maintenance 3～5 times/day of defecating 3～5.Treat 17 examples, all suffered from abdominal pain after 3～5 days and fade away, temperature recovery was normal on 3rd～7, and it is the longest 39 days, the shortest 5 days to be in hospital, average 14.9 days.
2.2 pyogenic cholangitis hyperpyrexia: with bavin yellow stuff (Radix Et Rhizoma Rhei, Radix Bupleuri, Natrii Sulfas, the Radix Paeoniae Alba, the Radix Aucklandiae) treatment.
2.3 blood stasis hyperpyrexia: treat with Taoren Chengqi Tang.
2.4 infantile fever caused by exogenous pathogens: grind into powder with the single Radix Et Rhizoma Rhei, be made into 20% Radix Et Rhizoma Rhei powder aqueous solution, each consumption baby is 15ml, and the child is 20ml, preschooler is 25ml, school age population is 30ml, retention enema, clinical treatment infantile fever caused by exogenous pathogens 60 examples, produce effects 40 examples, effective 18 examples, invalid 2 examples, total effective rate is 96.7%.
The 3 hemostasis effects of invigorating blood circulation
Experiment showed, oral or the external Radix Et Rhizoma Rhei, the hemorrhage and clotting time of people and laboratory animal all is significantly shorter than not medication person.Radix Et Rhizoma Rhei can shorten clotting time, reduces capillary permeability, improves vascular fragility, and the platelet Fibrinogen is increased, and the contraction of blood vessel is active to be increased, and promotes bone marrow to make platelet, therefore promotes blood coagulation.Radix Et Rhizoma Rhei can also suppress upper gastrointestinal motion, shortens clotting time.The hemostasis composition of Radix Et Rhizoma Rhei is chrysophanol, physcione, gallic acid etc.Radix Et Rhizoma Rhei can also promote that by its osmotic effect interstitial fluid shifts in blood vessel in addition, thereby makes hemodilution, and packed cell volume and blood viscosity descend.
3.1 upper gastrointestinal hemorrhage: single Radix Et Rhizoma Rhei powder 3g every day, treatment upper gastrointestinal hemorrhage 890 examples (liver cirrhosis cause hemorrhage except), hemostatic efficiency reaches 97%, average bleeding stopping period 2 days, medication 18g per capita.The bleeding stopping period of Radix Et Rhizoma Rhei obviously is better than Radix Et Rhizoma Rhei.
3.2 gastric ulcer acute perforation: with Radix Et Rhizoma Rhei compatibility Radix Scutellariae, Fructus Gardeniae, Flos Lonicerae, Fructus Forsythiae, the Radix Paeoniae Alba, Fructus Aurantii, Radix Glycyrrhizae, Os Sepiae.
3.3 hemobilia: get single Radix Et Rhizoma Rhei 20～30g, 2 doses of every days, decocting 5min, cool back is for oral administration, uses antibiotic, fluid infusion, treatment of blood transfusion simultaneously.Through controlling 1～5, take medicine after 2～13 doses, hemobilia all stops, and follows up a case by regular visits to 6～60, only 1 example is in more back recurrence in March, after still cure with this medicine.
3.4 metrorrhagia: add yellow wine with Radix Et Rhizoma Rhei decocting liquid, treatment dysfunctional uterine hemorrhage 48 examples, every day 2 times, 1～3 day course of treatment, 44 examples of fully recovering, cure rate this 91.7%, produce effects 4 examples, total effective rate is 100%.
3.5 bladder hemorrhage: adopt the irrigation of bladder of 1: 1 Radix Et Rhizoma Rhei leachate, every day 1 time, each 20ml, keep more than the 2h, treat obvious gross hematuria patient 15 examples, urine examination erythrocyte extinction time was less than 2 days 3 examples as a result, 3～4 days 10 examples, greater than 4 days 2 examples, average bleeding stopping period 3 days.
3.6 Radix Et Rhizoma Rhei also can be used for treating spitting of blood, epistaxis, bleeding hemorrhoids etc.
4 antibacterial actions
Radix Et Rhizoma Rhei all has in various degree inhibitory action to various bacteria, and is wherein the most responsive to staphylococcus, gonorrhea diplococcus.The Main Ingredients and Appearance of Radix Et Rhizoma Rhei antibacterial action is an anthraquinone derivatives, and is wherein the strongest with 3-carboxyl rhein, hydroxyl aloe-emodin, hydroxyemodin three antibacterial action.Its antibiotic mechanism mainly is to suppress oxidation, dehydrogenation, the deamination of thalline sugar and carbohydrate metabolism intermediate product, and energy Profilin matter and nucleic acid is synthetic.
4.1 acute biliary infection, cholecystitis: single Radix Et Rhizoma Rhei 30～60g decocting is for oral administration, per 1～2h1 time, and until defecation 5～6 times, after diseases such as stomachache alleviate, decrement gradually.
4.2 acute bacillary dysentery, acute enteritis, chronic colitis: oral Radix Et Rhizoma Rhei alcohol extraction sheet treatment acute enteritis 54 examples, cured in average 1.5 days; Treatment acute bacillary dysentery 110 examples, stool recovered normal time average 3.4 days, antibacterial clearance time 8.4 days, total effective rate 95%; Treatment chronic colitis Radix Et Rhizoma Rhei, the retention enema of Fructus Evodiae decocting liquid.
4.3 suppurative tonsillitis: Radix Et Rhizoma Rhei 20g dries, and grinds to the powder, and it is standby to bottle, and gets its 1/3 or 1/4 usefulness vinegar or tea furnishing pasty state at every turn, spreads out on calico or gauze, sticks arch of the foot (man a left side, woman right), wrapping 8h just can, every day 1 time, continuous 3～4 times.Treatment children's acute suppurative tonsillitis 30 examples, effective percentage 100%, cure rate 94%.
4.4 acute mastitis: Radix Et Rhizoma Rhei, Natrii Sulfas equal portions, grind into powder, add a small amount of vaseline, transfer evenly with boiled water, spread out on gauze, be affixed on the red and swollen position of breast, change dressings every day 3～4 times.If with aversion to cold, heating, weak symptom, can add the wuwei xiaodu drink plus-minus, wherein light disease person 16 examples, mean treatment 2.9 days; Serious symptom person's 14 examples, mean treatment 6.3 days is all cured.
4.5 oral disease: the swelling and aching of gum burning pain is treated with Radix Et Rhizoma Rhei, Radix Scrophulariae, the Radix Angelicae Dahuricae, Gypsum Fibrosum, Radix Achyranthis Bidentatae, Flos Lonicerae, Cortex Moutan, Fructus Piperis Longi, Fructus Xanthii, the Radix Rehmanniae, Herba Menthae; Aphtha is ground into powder with Radix Et Rhizoma Rhei, dried Alumen and is coated with the oral cavity.
4.6 ophthalmic diseases: acute conjunctivitis is treated with Radix Et Rhizoma Rhei compatibility Flos Lonicerae, Radix Scutellariae, Fructus Forsythiae, Fructus Gardeniae, Radix Platycodonis, Rhizoma Coptidis, Cortex Phellodendri, Radix Gentianae, Semen Cassiae, Herba Violae, Herba Taraxaci; Hordeolum grinds to the powder altogether to transfer with Radix Et Rhizoma Rhei, Scorpio, Flos Lonicerae, Flos Chrysanthemi, Rhizoma Coptidis, Radix Glycyrrhizae and is coated with the affected part.
5 protect the liver, spasmolytic function of gallbladder promoting, cholesterol reducing effect
The clinical treatment that is used for acute icterohepatitisshock, chronic hepatitis, hyperbilirubinemia, hepatic calculus, hyperlipemia.
6 diuretic antihypertensive effects
Radix Et Rhizoma Rhei and chrysophanic acid, emodin all have diuresis, the urine amount is increased, and promote ureteral wriggling, and sodium, potassium content increase and the effect of generation diuretic antihypertensive in the urine.Clinically be applicable to diseases such as edema, tympanites, enlarged abdomen abdominal distention, edema of lower limbs, dysuria.
In addition, external can be treated traumatic infection, rosacea, seborrheic dermatitis, burn, cold injury, herpes zoster etc.
(according to Wen Feng " pharmacological action of Radix Et Rhizoma Rhei and clinical practice thereof "----" the Shanxi traditional Chinese medical science " 2000.616 (3) 53-54)
The medicinal history of Radix Et Rhizoma Rhei is long, has good DEVELOPMENT PROSPECT.Regrettably, very limited to Radix Et Rhizoma Rhei chemical constituent and active further investigation thereof both at home and abroad at present.
In the prior art, Radix Et Rhizoma Rhei uses and to have limited its extensive use clinically to a great extent, especially in the practice of handling some complex diseases (for example unknown cause alimentary toxicosis), its advantage be difficult to be effectively played.For example, studies show that Radix Et Rhizoma Rhei can effectively be treated the poisoning that is caused by bacterial infection, but the effectiveness of inflammation that non-bacterial infection is caused and/or compound inflammation is not obvious relatively.Though someone had once inquired into the pharmacological action of Radix Et Rhizoma Rhei on cellular level, up to now, nobody provides the foundation of science to the combined effect of Radix Et Rhizoma Rhei and other active component.
Therefore, those skilled in the art are readily appreciated that, also wish to develop urgently such Compositional type pharmaceutical composition; It can work in coordination with main effects such as the eliminating inflammation and expelling toxin that strengthens Radix Et Rhizoma Rhei, astringing to arrest bleeding, beauty treatment toxin expelling pointedly, simultaneously the inflammation of inflammation that can cause inflammation caused by bacterial infection, especially non-bacterial infection or compound complicated infectious disease of the digestive tract is effective, and can shorten the course of disease, reduce the incidence rate of complication, and use conveniently, have no adverse reaction, be worthy of popularization.
Activated carbon (medicinal charcoal): gone up two ones of Chinese Pharmacopoeia versions in 2000.
Activated carbon (medicinal charcoal) is used for alimentary toxicosis, each toxoid of physical absorption, reduces the stimulation of each surface, offseting of toxoid road and ulcer surface, reduces each anatoxic net content of body class.Fibrous activated carbon and medicinal charcoal can also the motion of stimulating gastrointestinal road, directly facilitating digestion.
Summary of the invention
We have carried out deep research to the Radix Et Rhizoma Rhei and the mechanism of action thereof, particularly by scorching to xerosis cutis, gastric heat, coldness of the intestines to gastral stimulation, oral ulcer, halitosis, blood to having anti-alimentary tract inflammation and ulcer, removal and absorbing toxin, minimizing toxin; The beauty treatment eliminating toxin and beautifying the skin; The screening and the prescription of the given activity composition of enhancing immunity effect address the above problem effectively, and the present invention has promptly been finished in further research on this basis.
Through a large amount of experiment screenings, the inventor is surprised to find, and activated carbon (medicinal charcoal) can significantly strengthen the beneficial effect of Radix Et Rhizoma Rhei, and the compound artifact that comprises Radix Et Rhizoma Rhei and activated carbon (medicinal charcoal) simultaneously has significant treatment advantage than present commercially available prod.Activated carbon (medicinal charcoal) belongs to alimentary toxicosis medicine commonly used.Usually, activated carbon (medicinal charcoal) is independent application, cures mainly alimentary toxicosis, diarrhoea etc.Its physisorption that has at chemical pesticide and histamine is that other any article are irreplaceable, and the beneficial effect of coupling is remarkable.
Guiding test shows that Radix Et Rhizoma Rhei and activated carbon (medicinal charcoal) coupling shows synergism, significantly is better than and uses this two kinds of effectiveness that the single medicine is produced separately.Simultaneously, the inventor also is surprised to find, the two different proportioning (for example 1: 1-10: 1) and the relation between the effect be non-linear.For example, be that drug effect does not always show stronger situation along with the increase of Radix Et Rhizoma Rhei content in the scope of main effect (for example anastalsis) at the expection Radix Et Rhizoma Rhei; Similarly, in expection activated carbon (medicinal charcoal) is the scope of main effect (for example contratoxin adsorption), effect does not always strengthen along with the increase of carbon content yet.Especially it should be noted that in all guiding pharmacological evaluation and all found above-mentioned phenomenon, this obviously is different from the instruction of prior art.Radix Et Rhizoma Rhei stimulating gastrointestinal secretion back toxin is directly by firmly absorption of activated carbon (medicinal charcoal), toxin expelling and prevention intestinal secondary poisoning effect are obvious, because it is obvious to discharge the synergy of toxin, can be used for hepatitis, the auxiliary treatment of hypoimmunity, also can be used for prevention and treat all kinds of infectious intestinal diseases, because activated carbon itself has stronger adsorption to Radix Et Rhizoma Rhei, can alleviate the side effect of Radix Et Rhizoma Rhei to gastrointestinal irritation, therefore system carbon plate or carbon ball earlier during preparation, outsourcing Radix Et Rhizoma Rhei again, after allowing Radix Et Rhizoma Rhei play a role earlier, by activated carbon or medicinal charcoal absorption Radix Et Rhizoma Rhei and toxin, more desirable more again than simple mixing result of use.Through further investigation, determined that further the part by weight of Radix Et Rhizoma Rhei and activated carbon is 1 among the present invention: 1-10: 1, be preferably 1: 1-5: 1, more preferably 1: 1.
Therefore, an object of the present invention is to provide Radix Et Rhizoma Rhei and activated carbon (medicinal charcoal) coupling prevention or treatment alimentary canal inflammation, hemorrhage and/or alimentary toxicosis, oral ulcer, halitosis, blood scorching to xerosis cutis, gastric heat, coldness of the intestines relevant disease application and the effect of beauty treatment eliminating toxin and beautifying the skin and enhancing immunity.The present invention property created ground first proposes to unite and gives Radix Et Rhizoma Rhei and the above-mentioned disease that causes owing to many complicated reasons can be treated and/or control to activated carbon (medicinal charcoal) effectively, provide a kind of new therapeutic scheme as the substituting of prior art, this therapy will be that the doctor highly approves and puts forth effort and recommends.Described disease includes but not limited to Radix Et Rhizoma Rhei and the known kinds of Diseases that can treat or prevent of activated carbon (medicinal charcoal).Described disease also comprises the inflammation that non-bacterial infection causes, for example various chemical pesticides are poisoned.Described unite comprise the order use the preparation that comprises Radix Et Rhizoma Rhei, activated carbon (medicinal charcoal) respectively, also comprise and use the pharmaceutical preparation that contains Radix Et Rhizoma Rhei, activated carbon (medicinal charcoal) simultaneously.
On the other hand, the invention provides a kind of medicine or Halth-care composition, comprise that the Radix Et Rhizoma Rhei for the treatment of effective dose, activated carbon (medicinal charcoal) are as active component.In context, described Radix Et Rhizoma Rhei comprises the former powder or the extract of Radix Et Rhizoma Rhei, wherein extract comprises water and/or alcohol extract, also can be that the effective site of other specific solvent (petroleum ether, ether, chloroform, ethyl acetate, n-butyl alcohol) extraction or the combination of wherein single or several active component comprise emodin, chrysophanol, anthraquinone class, stilbene glycoside, chromone class, phenol glycoside, tannin etc.; Described activated carbon (medicinal charcoal) is meant and can be used for oral carbon, for example, considers from industrialization and economic aspect, can adopt following extraction process:
(1) Radix Et Rhizoma Rhei extracts: get Radix Et Rhizoma Rhei (coarse powder) 1000g, according to the percolation under fluid extract and the extractum item (appendix IO of Chinese Pharmacopoeia version in 2000), make solvent with 60% ethanol, flood after 24 hours, with the speed of per minute 1-3ml percolation slowly, collect the liquid 850ml that just filters, in addition device is preserved, and continues percolation, to the percolate color light till, collect the continuous liquid of filtering, be concentrated into the thick paste shape, add the liquid of just filtering, after the mixing, with 60% ethanol dilution to 1000ml, leave standstill, the clarification of waiting filters, drying under reduced pressure promptly gets (Chinese Pharmacopoeia 2000 version one one 356 pages).
(2) activated carbon (medicinal charcoal): by existing national standard (Chinese Pharmacopoeia 2000 version two ones 449 pages).
After preferred, medicine of the present invention or health-care food composition can be made oral double-deck dosage form, for example double-deck granule, bilayer tablet, double-deck capsule, double-deck drop pill, double-deck pilule, two-layer release-controlled dose, double-layer sustained release agent, outer rapid release agent, the agent of internal layer rapid release.Advantageously, in order to adapt to the treatment demand.In addition, also pharmaceutical composition of the present invention can be added in binder or the gauze for direct external.
The active component that is suitable for the above-mentioned dosage form of the present invention can be solid, powder or granule, liquid form.Particularly advantageously; can adopt equipment such as microjet granulator, jet mill; with microcrystalline Cellulose, PVP, CMC or PEG etc. is dispersant; Radix Et Rhizoma Rhei, activated carbon (medicinal charcoal) are made micron dimension (1-75 μ m particle diameter), sub-micrometer scale (100-500nm particle diameter) or thinner ultra-fine grain; with its curative effect of further raising; reduce dosage (for example, being about the 20-80% of conventional powder consumption, preferred 35-60%).
The pharmacodynamics test example
Material
MedicineRadix Et Rhizoma Rhei activated carbon compound double-layer tablet of the present invention; Rhubarb tablets, specification 0.25g/ sheet; Medicinal carbon plate, specification 0.25g/ sheet; (be double-layer tablet to the Radix Et Rhizoma Rhei carbon plate at 1: 1; Other are simple mixing tab), specification 0.25g/ sheet, Hainan state auspicious hall company limited.
AnimalKunming mouse and SD rat provide by medical faunae center, Hubei Province; Japan large rabbit is provided by experimental animal center, Hainan Province.The quality certification number: mice 19-082, rat 19-083, rabbit 30-003.
1. to the influence of mice capillary permeability
90 of mices, male and female half and half, body weight 18-22g is divided into 8 groups at random: (1) matched group (giving the isometric(al) distilled water); (2) aspirin group 0.2g/kg; (3)-(9) group gives compound rubarb, activated carbon (medicinal charcoal) the sheet 2.0g/kg of rhubarb tablets, carbon plate and different proportionings respectively.Each organizes PO administration every day 1 time, for three days on end, the blue normal saline solution 10mL/kg of the ivens of each Mus intravenous injection 0.1% of 30min after the last administration, every gives 0.6% glacial acetic acid (HAC) normal saline 0.2mL immediately, taking off cervical vertebra behind the 15min puts to death, every mice is washed abdominal cavity and sucking-off cleaning mixture for several times with the 6mL normal saline, adds normal saline after the merging to 10mL, and centrifuged supernatant is surveyed its optical density (OD) value in 722 spectrophotometers (590nm).
The influence that table 1 medicine increases the HAC induced mice abdominal cavity blood capillary general character (x ± s)
A: compare p＜0.05 with matched group; B: compare p＜0.05 with the rhubarb tablets group; C: compare p＜0.05 with activated carbon (medicinal charcoal) sheet group.
2. the influence of xylol induced mice ear swelling
Mice group and administration are with experiment 1,1h after the last administration, with dimethylbenzene 0.05mL/ only drip to mouse right ear, left ear compares, and puts to death animal behind the 15min, card punch with diameter 6mm downcuts ears with the position, be to weigh on the rigorous analysis balance of 1mg at sensibility reciprocal,, count out and respectively organize the swelling degree with heavy poor of left and right sides auricle, obtain inhibitory rate of intumesce (%), and the comparable group differences.
Table 2 medicine is to the influence (n=10) of mice ear (X ± S)
A: compare p＜0.05 with matched group; B: compare p＜0.05 with the rhubarb tablets group; C: compare p＜0.05 with the carbon plate group.
3. to the bullate influence of rat paw due to the Ovum Gallus domesticus album
Body weight is 80 of the rats of 150-180g, and male and female half and half are divided 8 groups: (1) matched group (giving the isometric(al) distilled water) at random; (2) aspirin group 0.15g/kg; (3)-(8) group gives compound rubarb, activated carbon (medicinal charcoal) the sheet 2.0g/kg of rhubarb tablets, activated carbon (medicinal charcoal) sheet and different proportionings respectively.Each organizes PO administration every day 1 time, and for three days on end, 1h respectively organizes fresh albumen 0.05mL/ so that inflammation of injection 100% under the right back sufficient lift after the last administration; Different time caused the following change in volume in scorching sufficient sole of the foot joint before and after measurement caused inflammation, and consequently scorching forebody-afterbody product moment value is the swelling degree, and compares between organizing.
Table 3 medicine is to the influence (n=10) of rat paw edema due to the Ovum Gallus domesticus album (X ± S)
A: compare p＜0.05 with matched group; B: compare p＜0.05 with the rhubarb tablets group; C: compare p＜0.05 with the carbon plate group.
The result of experiment 1-3 shows that after rhubarb tablets and carbon plate were made compound double-layer tablet by 1: 1 proportioning, its antiinflammatory action was significantly strengthened, with Radix Et Rhizoma Rhei, activated carbon (medicinal charcoal) is compared has significant difference.Other four kinds of proportionings to a certain degree can strengthen its antiinflammatory action, but compare there was no significant difference with Radix Et Rhizoma Rhei, activated carbon (medicinal charcoal) sheet.
4. the Attenuation effect of adsorbing the intestines and stomach toxin
Test used clinical isolates strain and amount to 4 strains, be the clinical isolates strain of area, Changsha various big hospital.Every strain antibacterial is divided pure before experiment through agar plate, 37 ℃ of fresh cultured of spending the night are done and are used for experiment after the suitable dilution.Got in 2 hours after the rabbit model administration after rabbit gastric juice and the administration and to get that heartbroken liquid filters, centrifugal, dilution back adopts the HPLC method to measure stomach, enterogenous endotoxin content in 7 hours, the results are shown in Table 4.
The different proportioning Radix Et Rhizoma Rhei of table 4, activated carbon (medicinal charcoal) the effect measuring result behind the gastrointestinal that acts on
The result shows, for staphylococcus aureus, helicobacter pylori and escherichia coli, act on detoxifying function a little less than, but the attenuation best results of 1: 1 prescription of itself and activated carbon (medicinal charcoal), and the compound recipe sheet of 2: 1,4: 1,1: 2,1: 4 proportioning is compared with the effect of rhubarb tablets and is had certain effect; For Salmonella, the Radix Et Rhizoma Rhei of 1: 1,1: 2,1: 4 proportioning, activated carbon (medicinal charcoal) compound recipe sheet action effect are close, are better than rhubarb tablets, carbon plate.
5. refrigeration function
Get 24 of rabbit, 24 ℃ of room temperatures, 2d surveys anus temperature 4 times every day before the experiment, selects body temperature to be no more than 0.3 ℃ animal for experiment usefulness 39.0-39.8 ℃ and body temperature fluctuation every day.After surveying the basal body temperature of animal morning experiment day, be divided into totally 3 groups of Radix Et Rhizoma Rhei, activated carbon (medicinal charcoal) compound recipe sheet 1.2g/kg, 0.6g/kg and matched groups (giving equal-volume 0.5% carboxymethylcellulose sodium solution) at random, every group 8, press the 10ml/kg gastric infusion respectively 1 time after pressing 0.5ml/kg auricular vein injection with the typhoid Vi polysaccharide vaccine, then every 1h survey anus temperature 1 time.
The influence (n=8) that table 5 medicine raises to rabbit body temperature due to the rabbit typhoid Vi polysaccharide vaccine (X ± S)
Annotate: the t check, compare * p＜0.05 with matched group
The result shows, Radix Et Rhizoma Rhei, activated carbon (medicinal charcoal) sheet 1.2g/kg, 0.6g/kg and matched group fervescence be 3h arrival peak after giving vaccine inoculation all, administration group body temperature is respectively 39.2 ± 0.7 ℃ and 39.7 ± 0.6 ℃, matched group body temperature is 41.2 ± 0.7 ℃, shows that Radix Et Rhizoma Rhei, activated carbon (medicinal charcoal) sheet have certain refrigeration function to the rabbit body temperature rising due to the typhoid Vi polysaccharide vaccine.
6. analgesic activity
Mice group and administration are with experiment 1, and to give concentration be 0.7%HAC normal saline 0.1ml lumbar injection to 30min after the last administration, observe in the 15min animal and turn round the body number of times, with matched group comparable group differences.
Table 6 Radix Et Rhizoma Rhei, activated carbon (medicinal charcoal) sheet are to the influence (n=10) of the mouse writhing due to the glacial acetic acid
Annotate: * and matched group are relatively
The result shows, Radix Et Rhizoma Rhei, activated carbon (medicinal charcoal) sheet 1.2g/kg, 0.6g/kg and three dosage groups of 0.3g/kg all have the inhibitory action of highly significant to the mouse writhing due to the glacial acetic acid.
7. to the influence of mice non-specific immunity (phagocytic function)
Mice group and administration are with experiment 1, behind each treated animal administration 7d, after the tail vein is pressed the india ink of 0.1ml/10g injection 10%, get blood 20 μ l in 2min and 10mi n eye socket, be added to lysed erythrocyte in the 4ml distilled water, use 722 type spectrophotometers (600nm) and measure optical density (OD) value, with OD value and matched group comparable group differences.
The result shows that Radix Et Rhizoma Rhei, activated carbon (medicinal charcoal) sheet 1.2g/kg, 0.6g/kg and three dosage groups of 0.3g/kg all can obviously strengthen the non-specific function of mice.
Table 7 Radix Et Rhizoma Rhei, activated carbon (medicinal charcoal) sheet are to the influence of mice reticuloendothelial system phagocytic function (X ± S)
Annotate: the t check, compare * P＜0.05, * * P＜0.01 with matched group
8. to the influence of mice delayed allergy (DTH)
Mice group and medication be experiment 1 together, successive administration 5d, and in administration 3d tail vein injection sheep red blood cell sensitization, administration 5d compares with volume normal saline injection right side sole with sheep red blood cell injection left side sole.Measure two sole thickness after 24 hours, with both difference and matched group comparable group differences.
Table 8 Radix Et Rhizoma Rhei, activated carbon (medicinal charcoal) sheet are to mouse cell Immune Effects (n=10)
Annotate: * and matched group are relatively.
The result shows that Radix Et Rhizoma Rhei, activated carbon (medicinal charcoal) sheet 1.2g/kg, 0.6g/kg and three dosage groups of 0.3g/kg all can obviously improve cell immune function of human body.
The specific embodiment
Example of formulations
Embodiment 1 tablet
Exemplary process: after getting activated carbon (medicinal charcoal) and sieving, 60 ℃ of dryings, tabletting.The gained tablet can further be pressed double-layer tablet.Get Radix Et Rhizoma Rhei extract, hydroxypropyl cellulose, magnesium stearate and be pressed into double-layer tablet with active carbon plate, reuse Opadry coating material package clothing after with 75% alcohol granulation.
Embodiment 2 hard capsules
Exemplary process: after getting activated carbon or medicinal charcoal and sieving, 60 ℃ of dryings, pill.Filling Capsules after gained ball bag Radix Et Rhizoma Rhei extract, hydroxypropyl cellulose, magnesium stearate are granulated with 75% ethanol again.
Claims (3)
1. a medicine or health-care food composition with antiinflammatory action, it is characterized in that active component is Radix Et Rhizoma Rhei and medicinal charcoal, Radix Et Rhizoma Rhei and medicinal charcoal weight proportion are 1: 1-10: 1, described Radix Et Rhizoma Rhei is: the former powder of Radix Et Rhizoma Rhei or the water and/or the alcohol extract of Radix Et Rhizoma Rhei, the perhaps effective site of the petroleum ether of Radix Et Rhizoma Rhei, ether, chloroform, ethyl acetate extraction, first in the preparation pharmaceutical purpose carbon is internal layer, wraps Radix Et Rhizoma Rhei again for outer.
2. according to the medicine or the health-care food composition of claim 1, wherein Radix Et Rhizoma Rhei and medicinal charcoal part by weight are 1: 1.
3. according to each medicine or health-care food composition of claim 1-2, its Chinese medicine is the dosage form of oral administration.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005100019865A CN100441166C (en) | 2005-01-17 | 2005-01-17 | Combination of medication of containing medicinal rhubarb and activated carbon or carbo medicinalis |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005100019865A CN100441166C (en) | 2005-01-17 | 2005-01-17 | Combination of medication of containing medicinal rhubarb and activated carbon or carbo medicinalis |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1660350A CN1660350A (en) | 2005-08-31 |
| CN100441166C true CN100441166C (en) | 2008-12-10 |
Family
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB2005100019865A Expired - Fee Related CN100441166C (en) | 2005-01-17 | 2005-01-17 | Combination of medication of containing medicinal rhubarb and activated carbon or carbo medicinalis |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN100441166C (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB0516069D0 (en) * | 2005-08-04 | 2005-09-14 | Imp College Innovations Ltd | Pharmaceutical and use thereof |
| US8048413B2 (en) * | 2006-05-17 | 2011-11-01 | Helene Huguet | Site-specific intestinal delivery of adsorbents, alone or in combination with degrading molecules |
| JP5781164B2 (en) | 2011-10-07 | 2015-09-16 | 帝人ファーマ株式会社 | Adsorbent for oral administration |
-
2005
- 2005-01-17 CN CNB2005100019865A patent/CN100441166C/en not_active Expired - Fee Related
Non-Patent Citations (4)
| Title |
|---|
| 大黄、活性炭复方制剂对7/8肾切除大鼠慢性肾功能不全模型的治疗效果. 金华等.延边大学医学学报，第22卷第2期. 1999 |
| 大黄、活性炭复方制剂对7/8肾切除大鼠慢性肾功能不全模型的治疗效果. 金华等.延边大学医学学报，第22卷第2期. 1999 * |
| 活性炭的临床应用. 曾钟波.实用临床医学，第5卷第2期. 2004 |
| 活性炭的临床应用. 曾钟波.实用临床医学，第5卷第2期. 2004 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1660350A (en) | 2005-08-31 |
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Assignee: HAINAN GRTANG TRADITIONAL CHINESE MEDICINE PHARMACEUTICAL CO., LTD. Assignor: Yan Bin Contract record no.: 2010990000965 Denomination of invention: Combination of medication of containing medicinal rhubarb and activated carbon or carbo medicinalis Granted publication date: 20081210 License type: Exclusive License Open date: 20050831 Record date: 20101209 |
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