CN104324329A

CN104324329A - Mulberry oral liquid with effects of soothing nerves and nourishing brain and preparation method of mulberry oral liquid

Info

Publication number: CN104324329A
Application number: CN201410665604.8A
Authority: CN
Inventors: 不公告发明人
Original assignee: Qingdao Hengbo Instrument Co Ltd
Current assignee: Qingdao Hengbo Instrument Co Ltd
Priority date: 2014-11-20
Filing date: 2014-11-20
Publication date: 2015-02-04

Abstract

The invention discloses mulberry oral liquid with effects of soothing nerves and nourishing brain. The oral liquid with effects of soothing nerves and nourishing brain comprises the following various raw materials in parts by weight: 10-50 parts of mulberry, 10-20 parts of polygala tenuifolia, 10-20 parts of tuber fleeceflower stem, 10-20 parts of cortex lycii radicis, 10-20 parts of glossy privet fruit, 10-20 parts of poria with hostwood, 10-20 parts of nacre mother of pearl, 10-20 parts of raspberry, 10-20 parts of jasmine, 10-20 parts of red paeony root, 10-20 parts of radix curcumae, 10-20 parts of spina date seed, 10-20 parts of cortex albiziae, 10-20 parts of bighead atractylodes rhizome, 10-20 parts of rhizoma polygonati, 10-20 parts of motherwort, 10-20 parts of radix bupleuri, 10-20 parts of prepared fleece flower root and 10-20 parts of herba epimedii. The mulberry has the effects of improving the skin blood supply, nourishing the skin and tonifying liver and tonifying the kidney and can delay senescence. The oral liquid disclosed by the invention is compound oral liquid prepared from medicines such as the mulberry, polygala tenuifolia, prepared fleece flower root, herba epimedii, motherwort, glossy privet fruit and red paeony root, has the effects of nourishing brain and soothing the nerves, promoting essence granulation and supplementing marrow, clearing liver and improving vision and reinforcing qi and nourishing blood and is used for treating symptoms such as neurasthenia, insomnia, amnesia and dizziness.

Description

Fructus Mori oral liquid of a kind of Anshen Bunao and preparation method thereof

Technical field

The present invention relates to the health product containing deriving from plant, animal or mineral component, particularly a kind of oral liquid and preparation method thereof of Fructus Mori of Anshen Bunao.

Background technology

Hunt for a job, promote, house, pay rise, sufferer, child education, every aspect life trival matters make modern worn out mentally and physically, sensation health unable to make ends meet.Add modern metropolitan cities working clan and generally use computer office, tobacco and wine are immoderate, and night life is uncontrolled, stay up late, and these have all directly injured Liver and kidney.Watching for a long time and radiation, the eye health for computer gang has buried hidden danger.This kind of people needs hepatoprotective while attention eye hygiene to nourish blood.The living and diet of modern people is too meticulous, and quantity of motion reduces, or eating and drinking too much at one meal, or overstrain, body metabolism function is failed gradually, causes visceral dysfunction, easily produce sleeping problems.Want to sleep well may become a lot of white collar, the extravagant hope of the gang that requires mental skill.

Fructus Mori are the mature fruit of Moraceae deciduous tree mulberry, and Fructus Mori are again sorosis, Mulberry Fructus Jujubae, and peasant likes the fresh fruit of its maturation to eat, and taste sweet juice is many, is one of fruit of often eating of people.Ripe Fructus Mori matter is glossy, sour and sweet palatability, with individual large, meat is thick, color is purplish red, the sufficient person of sugar is good.Gather during annual fruit maturation in 4 ~ June, clean, decontamination, dry or dry edible after summary steaming.

Before more than 2,000 years, Fructus Mori have been the hired tonic of Chinese emperors.Because growing environment that mulberry is special makes sorosis have spontaneous growth, feature without any pollution, so Fructus Mori are otherwise known as " holy fruit among the people ".Modern study confirms, containing the abundant composition such as activated protein, vitamin, aminoacid, carotene, mineral, resveratrol, anthocyanidin in Fructus Mori fruit, nutrition is 5 ~ 6 times of Fructus Mali pumilae, it is 4 times of Fructus Vitis viniferae, there is multiple efficacies, be described as " the best health fruit of 21st century " by medical circle.Often eat Fructus Mori and can significantly improve body immunity, there is slow down aging, effect of looks improving and the skin nourishing.

Chinese medicine thinks Fructus Mori sour in the mouth, cold nature, have improve body immunity, the effect such as dry is dispelled in YIN nourishing of enriching blood, promoting the production of body fluid to quench thirst, intestine moistening, cure mainly deficiency of YIN-blood and cause have a dizzy spell, tinnitus cardiopalmus, irritated insomnia, soreness of the waist and knees, early whitening of beard and hair, quench one's thirst only do, constipation with dry stool, nourishing YIN and supplementing blood, Anshen Bunao, sharp joint, curing rheumatism, the effect such as to relieve the effect of alcohol.

1, sclerosis of blood vessels is prevented: containing fatty acid in Fructus Mori, primarily of linoleic acid.Stearic acid and oleic acid composition, have and reduce fat, reduce blood fat, prevent the effects such as sclerosis of blood vessels.

2, strengthening the spleen and stomach, aid digestion: containing nutrient substance such as tannic acid, fatty acid, malic acids in Fructus Mori, the digestion of fat, protein and starch can be helped, therefore the merit having strengthening the spleen and stomach aid digestion, can be used for treating the diarrhoea caused because of dyspepsia.

3, supplement the nutrients: the trace element etc. containing a large amount of moisture, carbohydrate, multivitamin, carotene and needed by human in Fructus Mori, effectively can expand the blood volume of human body, and to mend and oiliness, be suitable for hypertension, gynaecological disease patient dietetic therapy.

4, blacking hair and beauty: containing the nutrient substance that a large amount of needed by human body is wanted in Fructus Mori, also containing black hair element, can make hair become black and glossy, can be used to beauty treatment.

Fructus Mori, having another name called Fructus Mori, Mulberry sugarcane, Mulberry Fructus Jujubae, sorosis, black mulberry etc., is the fruit ear of moraceae plants Mulberry.When Fructus Mori is tender, color is blue or green, and sour in the mouth, time aging, color purple is black, succulence, and taste is sweet.Ripe Mulberry fruit is nutritious, every 100 grams of Fructus Mori moisture contents 81.8 grams, 1.8 grams, protein, 0.3 gram, fat.Cellulose 4.9 grams, carbohydrate 10 grams, ash 1.2 grams, carotene 30 microgram, thiamine 0.02 milligram, 0.06 milligram, riboflavin, vitamin E 6.95 milligrams, 33 milligrams, potassium, 0.27 milligram, zinc, copper 0.08 milligram, selenium 4.8 microgram.In addition, also containing tannic acid, malic acid, vitamin C and fatty acid etc.Its fat is mainly linoleic acid, oleic acid, palmitic acid, stearic acid and sad, n-nonanoic acid, capric acid, myristic acid, linolenic acid etc. on a small quantity.Chinese medicine thinks Fructus Mori liver and kidney tonifying, nourishing YIN and benefiting blood, endogenous wind stopping, have cure mainly palpitation and insomnia, have a dizzy spell, tinnitus, constipation night sweat, scrofula, the disease such as articular instability.

Fructus Mori are improved skin (comprising scalp) blood supply, nutrition skin, make the effects such as the delicate and black hair of skin, and can slow down aging.Fructus Mori are that middle-aged and elderly people is healthy and strong U.S. face, antidotal good fruit and good medicine.Normal food Fructus Mori can improving eyesight, the symptom that relieving eye strain is dry and astringent.Fructus Mori have immunologic enhancement.Fructus Mori have effect of gain to spleen, have potentiation to hemolytic reaction, can prevent human body artery sclerosis, skeletal joint sclerosis, enhance metabolism.It can promote the growth of erythrocyte, prevents leukopenia, and has auxiliary effect to diseases such as treatment diabetes, anemia, hypertension, hyperlipidemia, coronary heart disease, neurasthenias.Fructus Mori has the effects such as promoting the production of body fluid to quench thirst, facilitating digestion, help defecation, and proper amount of edible can promote gastric secretion, stimulates enterokinesia and removes scorching.Motherland's medical science is thought, Fructus Mori are sweet in flavor and cold in property, there is the liver and the kidney tonifying, intestine moistening of promoting the production of body fluid, the effect such as black hair improving eyesight.

Various nourishing the liver hepatoprotective, the health product of Anshen Bunao, medicine spread unchecked on the market now, but great majority are had a good apperance only, false propaganda on a grand scale, probably without any health care, but cause the huge injury of the person, therefore, the health product of the effective hepatoprotective of search of health, Anshen Bunao have very real significance.Chinese medicine hepatoprotective, Anshen Bunao have advantage that is nontoxic, that can be absorbed completely by organism, are the Main way that we study.

Hunt for a job, promote, house, pay rise, sufferer, child education, every aspect life trival matters make modern worn out mentally and physically, sensation health unable to make ends meet; A lot of people feel all not sleep well is made at urban life pressure; Add modern metropolitan cities working clan and generally use computer office watching for a long time, tobacco and wine are immoderate, the behavior of the injury Liver and kidney such as night life is uncontrolled, stay up late, the provisions that eyes are subject to blood just can look thing, and excessively use eye, can liver-blood deficiency be made, make binocular can not get the supply of nutrition, thus go out to lose face dry and astringent, see thing is fuzzy, nyctalopia etc.In addition, long-term sitting eye, except binocular blood supply insufficiency, cervical vertebra, lumbar vertebra also can produce strain, less than alleviation, also can must cause damage to liver.If probably be difficult to adhere to by Drug therapy simple, carelessly use collyrium more can make eyes damaged, cause various oculopathy or blind danger, and it is cumbersome to take Chinese medicine, mouthfeel is not good, unsuitable long-term taking, and effect is also not necessarily desirable.

Summary of the invention

Various nourishing the liver hepatoprotective, the health product of Anshen Bunao, medicine spread unchecked on the market now, but great majority are had a good apperance only, false propaganda on a grand scale, probably without any health care, but cause the huge injury of the person, therefore, the health product of the effective hepatoprotective of search of health, Anshen Bunao have very real significance.Chinese medicine hepatoprotective, Anshen Bunao have advantage that is nontoxic, that can be absorbed completely by organism, are the Main way that we study.Chinese medicine thinks Fructus Mori liver and kidney tonifying, nourishing YIN and benefiting blood, endogenous wind stopping, have cure mainly palpitation and insomnia, have a dizzy spell, tinnitus, constipation night sweat, scrofula, the disease such as articular instability; Fructus Mori are improved skin blood supply, nutrition skin, make the effects such as the delicate and black hair of skin, and can slow down aging.Normal food Fructus Mori can improving eyesight, the symptom that relieving eye strain is dry and astringent.Fructus Mori have immunologic enhancement.Fructus Mori have effect of gain to spleen, have potentiation to hemolytic reaction, can prevent human body artery sclerosis, skeletal joint sclerosis, enhance metabolism; Check melanin grows, and keeps skin gloss and elasticity to play no small effect.The present invention is the health product of green health environmental protection completely.The present invention is the compound oral liquid be made up of medicines such as Fructus Mori, Radix Polygalae, Radix Polygoni Multiflori Preparata, Herba Epimedii, Herba Leonuri, Fructus Ligustri Lucidi, Radix Paeoniae Rubra, there is invigorating brain and relieving mental uneasiness, spermatogenesis mends marrow, effect of liver heat removing and eyesight improving benefiting QI and nourishing blood, for neurasthenia, insomnia, the disease such as forgetful, dizzy.

For solving above-mentioned technical barrier, the invention provides a kind of Fructus Mori oral liquid of Anshen Bunao, in Anshen Bunao oral liquid described in it, the parts by weight of various raw material can be: Fructus Mori 10 ~ 50 parts, Radix Polygalae 10 ~ 20 parts, Caulis Polygoni Multiflori 10 ~ 20 parts, Cortex Lycii 10 ~ 20 parts, Fructus Ligustri Lucidi 10 ~ 20 parts, Poria cum Radix Pini 10 ~ 20 parts, Concha Margaritifera 10 ~ 20 parts, Fructus Rubi 10 ~ 20 parts, Fructus Gardeniae 10 ~ 20 parts, Radix Paeoniae Rubra 10 ~ 20 parts, Radix Curcumae 10 ~ 20 parts, Semen Ziziphi Spinosae 10 ~ 20 parts, Cortex Albiziae 10 ~ 20 parts, the Rhizoma Atractylodis Macrocephalae 10 ~ 20 parts, Rhizoma Polygonati 10 ~ 20 parts, Herba Leonuri 10 ~ 20 parts, Radix Bupleuri 10 ~ 20 parts, Radix Polygoni Multiflori Preparata 10 ~ 20 parts, Herba Epimedii 10 ~ 20 parts.

The Fructus Mori oral liquid of described Anshen Bunao, in Anshen Bunao oral liquid described in it, the parts by weight of various raw material can also be: Fructus Mori 10 ~ 30 parts, Radix Polygalae 10 ~ 15 parts, Caulis Polygoni Multiflori 10 ~ 15 parts, Cortex Lycii 10 ~ 15 parts, Fructus Ligustri Lucidi 10 ~ 15 parts, Poria cum Radix Pini 10 ~ 15 parts, Concha Margaritifera 10 ~ 15 parts, Fructus Rubi 10 ~ 15 parts, Fructus Gardeniae 10 ~ 15 parts, Radix Paeoniae Rubra 10 ~ 15 parts, Radix Curcumae 10 ~ 20 parts, Semen Ziziphi Spinosae 10 ~ 20 parts, Cortex Albiziae 10 ~ 20 parts, the Rhizoma Atractylodis Macrocephalae 10 ~ 20 parts, Rhizoma Polygonati 10 ~ 20 parts, Herba Leonuri 10 ~ 20 parts, Radix Bupleuri 10 ~ 20 parts, Radix Polygoni Multiflori Preparata 10 ~ 20 parts, Herba Epimedii 10 ~ 20 parts.

The Fructus Mori oral liquid of described Anshen Bunao, in Anshen Bunao oral liquid described in it, the parts by weight of various raw material also can be: Fructus Mori 10 ~ 40 parts, Radix Polygalae 10 ~ 20 parts, Caulis Polygoni Multiflori 10 ~ 20 parts, Cortex Lycii 10 ~ 20 parts, Fructus Ligustri Lucidi 10 ~ 20 parts, Poria cum Radix Pini 10 ~ 20 parts, Concha Margaritifera 10 ~ 20 parts, Fructus Rubi 10 ~ 20 parts, Fructus Gardeniae 10 ~ 20 parts, Radix Paeoniae Rubra 10 ~ 20 parts, Radix Curcumae 10 ~ 15 parts, Semen Ziziphi Spinosae 10 ~ 15 parts, Cortex Albiziae 10 ~ 15 parts, the Rhizoma Atractylodis Macrocephalae 10 ~ 15 parts, Rhizoma Polygonati 10 ~ 15 parts, Herba Leonuri 10 ~ 15 parts, Radix Bupleuri 10 ~ 15 parts, Radix Polygoni Multiflori Preparata 10 ~ 15 parts, Herba Epimedii 10 ~ 15 parts.

For solving the problems of the technologies described above, the present invention also provides a kind of preparation method of Fructus Mori oral liquid of Anshen Bunao, and the preparation process of the Fructus Mori oral liquid of Anshen Bunao described in it comprises following aspect:

A. the extraction of Fructus Mori;

B. the extraction of other crude drug;

C. above-mentioned two kinds of extracting solution are merged concentrated; Sterilizing, adds Mel seasoning and is prepared into oral liquid.

In described step a, can gather during annual fruit maturation in 4 ~ June, clean, decontamination, dry or omit after steaming and dry.

In described step a, may further include and the Fructus Mori dried are put into water, heating and refluxing extraction 2 times, each 1 ~ 2 hour, 2 extracting solution are merged and leaves standstill; Again the Fructus Mori medicinal residues that above-mentioned water extraction is crossed are added alcohol heating reflux and extract 2 times, each 1 ~ 2 hour, 2 extracting solution are merged and leaves standstill; Merged by above-mentioned two kinds of extracting solution, decompression recycling ethanol, obtains Fructus Mori extracting solution.

In described step b, other raw materials can be cleaned roguing, dry, be ground into granule, steep in water, soak more than 2 hours, add the water extraction 2 times of 8 times amount of crude drug gross weight, 6 times amount respectively, extraction time is 2 hours, 1 hour; Merge 2 extracting solution, extracting solution is centrifugal by tubular type, obtains centrifugal liquid, and centrifugal liquid is crossed D 101 macroporous resin and adsorbed, and first washes remove impurity with water, then carries out eluting with the ethanol of 70%, collects eluent, concentrating under reduced pressure, one-tenth pasty state medicinal liquid.

In described step c, above-mentioned two kinds of extracting solution can be inserted in economic benefits and social benefits vacuum concentrator, when being concentrated into 90 DEG C, relative density is the concentrated solution of 1.25, sets to 0 ~ 5 DEG C of deepfreezes 24 hours; Cold preservation liquid is added the filter aid kieselguhr of 0.3%, filter, filtrate is inserted in economic benefits and social benefits vacuum concentrator again, is concentrated into every 1ml containing 1g crude drug amount, for subsequent use.

For solving the problems of the technologies described above, the present invention reoffers a kind of preparation method of Fructus Mori oral liquid of Anshen Bunao, and described in it, the preparation method of oral liquid comprises further:

A, get all raw materials and put into water and soak, heat is extracted 2 times, is component 1;

B, the medicinal residues of said extracted being added ethanol extraction 2 times, is component 2;

C, by 2-in-1 and concentrated to component 1 and component; Sterilize after adding dextrin mediation bottling.

In described step a, can get all crude drug and steep in water, soak more than 2 hours, add the water extraction 2 times of 8 times amount of crude drug gross weight, 6 times amount respectively, extraction time is 2 hours, 1 hour; Merge 2 extracting solution, extracting solution is centrifugal by tubular type, obtains centrifugal liquid, and centrifugal liquid is crossed D 101 macroporous resin and adsorbed, and first washes remove impurity with water, then carries out eluting with the ethanol of 70%, collects eluent, concentrating under reduced pressure, and one-tenth extractum, for component becomes 1; In described step b, the medicinal residues of crude drug said extracted can crossed add ethanol, and add the ethanol warm macerating 2 times of 10 times amount of medicinal residues gross weight, 8 times amount respectively, the warm macerating time is 2 hours, 1.5 hours, and temperature is 85 DEG C, merges 2 warm macerating liquid; Warm macerating liquid is evaporated to 1.25 extractum when density is 60 DEG C, and extractum is added the extraction into ethyl acetate secondary of its 4 times amount, extract has reclaimed ethyl acetate, becomes extractum, obtains component 2; In described step c, above-mentioned two kinds of extracting solution can be merged, when concentrating under reduced pressure relative density is 80 DEG C 1.35 filtrate, reclaim ethanol, inserted in economic benefits and social benefits vacuum concentrator by above-mentioned two kinds of extracting solution, when being concentrated into 90 DEG C, relative density is the concentrated solution of 1.60, sets to 0 ~ 5 DEG C of deepfreezes 24 hours; Cold preservation liquid is added the filter aid kieselguhr of 0.3%, filter, filtrate is inserted in economic benefits and social benefits vacuum concentrator again, is concentrated into every 1ml containing 1g crude drug amount, and sterilize after adding dextrin mediation bottling.。

The technique effect that the present invention is useful is, the present invention has advantage that is nontoxic, that can be absorbed completely by organism.Chinese medicine thinks Fructus Mori liver and kidney tonifying, nourishing YIN and benefiting blood, endogenous wind stopping, have cure mainly palpitation and insomnia, have a dizzy spell, tinnitus, constipation night sweat, scrofula, the disease such as articular instability; Fructus Mori are improved skin blood supply, nutrition skin, make the effects such as the delicate and black hair of skin, and can slow down aging.Fructus Mori are that middle-aged and elderly people is healthy and strong U.S. face, antidotal good fruit and good medicine.Normal food Fructus Mori can improving eyesight, the symptom that relieving eye strain is dry and astringent.Fructus Mori have immunologic enhancement.Fructus Mori have effect of gain to spleen, have potentiation to hemolytic reaction, can prevent human body artery sclerosis, skeletal joint sclerosis, enhance metabolism; Check melanin grows, and keeps skin gloss and elasticity to play no small effect.The present invention is the health product of green health environmental protection completely.Add some blood circulation promoting and blood stasis dispelling, the natural Chinese medicine of inrigorating qi and promoting blood circulation, dispersing the stagnated live-QI to relieve the stagnation of QI, suppressing the hyperactive liver and subsiding YANG, enriching yin and nourishing kidney, heat-clearing and toxic substances removing, clearing away heat-fire, strengthening vital QI to eliminate pathogenic factors, purging FU-organs lets out poison, promoting blood circulation to remove blood stasis, regulates the metabolism function of human body, improves blood circulation of human body and liver detoxification ability.The present invention is the compound oral liquid be made up of medicines such as Fructus Mori, Radix Polygalae, Radix Polygoni Multiflori Preparata, Herba Epimedii, Herba Leonuri, Fructus Ligustri Lucidi, Radix Paeoniae Rubra, there is invigorating brain and relieving mental uneasiness, spermatogenesis mends marrow, effect of liver heat removing and eyesight improving benefiting QI and nourishing blood, for neurasthenia, insomnia, the disease such as forgetful, dizzy.

Detailed description of the invention

Prevent sclerosis of blood vessels: containing fatty acid in Fructus Mori, primarily of linoleic acid.Stearic acid and oleic acid composition, have and reduce fat, reduce blood fat, prevent the effects such as sclerosis of blood vessels.

Rhizoma Atractylodis Macrocephalae bitter in the mouth, sweet, temperature.Return spleen, stomach warp.Cure mainly invigorating the spleen and benefiting QI, dampness diuretic, hidroschesis, antiabortive.For insufficiency of the spleen lack of appetite, abdominal distention is had loose bowels, phlegm retention vertigo and palpitation, edema, spontaneous perspiration, frequent fetal movement.The Rhizoma Atractylodis Macrocephalae has cytotoxicity to oncocyte, can reduce the rate of increase of oncocyte, lowers the aggressive of tumor tissue, improves the ability of body antitumor reaction.Rhizoma Atractylodis Macrocephalae volatile oil has inhibitory action to mice ehrlich carcinoma, lymphosarcoma ascitic type, the esophageal carcinoma (Eca109), sarcoma 180 etc.Atractylodes lactone B lumbar injection is to the murine sarcoma people.Also remarkable inhibitory action is had." property of medicine opinion " carries it: monarch, and sweet in the mouth is pungent, nontoxic.Energy main large migratory arthralgia, for many years diarrhea due to disorder of qi, trusted subordinate's distending pain, the broken dyspepsia that disappears, appetizing, removes sputum, except cold and heat, lets out only.Interarea light is pleased, and preserves youthful looks, de-black.Swelling of harnessing the river is full, tells vomiting, cold type of pain in abdomen, vomits and diarrhoea incessantly, and insufficiency of stomach-QI cold-dysentery.

Radix Curcumae is pungent, bitter, cold.Return liver, the heart, lung meridian.Function cures mainly regulating qi to disperse stagnation, heart fire-clearing upset-relieving, promoting the function of the gallbladder to alleviate jaundice.For amenorrhea dysmenorrhea, chest and abdomen swelling and pain, twinge, calentura coma, epilepsy is gone mad, jaundice dark coloured urine.Promoting QI circulation for relieving depression, removing heat from blood removing blood stasis with potent drugs.Control all pains of breast abdomen side of body rib, lose the heart demented, calentura coma, spit blood, epistaxis, hematuria, stranguria with blood, women's vicarious menstruation, jaundice." property of medicine opinion " carries it: control woman's stagnated blood epigastric pain caused by emotional factors, and cold air knot is poly-, and warm vinegar rubs and takes it.Tang Materia Medica carries it: main accumulation of blood, the therapeutic method to keep the adverse QI flowing downwards, granulation promoting, hemostasis, broken stagnant blood, stranguria with blood, hematuria, incised wound.

Herba Leonuri acrid in the mouth is bitter, cool.Invigorate blood circulation, silt of dispelling, regulating menstruation, disappear water.Treatment menoxenia, vaginal bleeding during pregnancy is had difficult labour, retention of placenta, puerperal blood faints, abdominal pain due to blood stasis, and metrorrhagia and metrostaxis, rushes down blood, carbuncle skin infection at hematuria.Herba Leonuri, containing the material such as leonurine, Fumaric acid, has the effects such as promoting blood flow to regulate menstruation, blood pressure lowering, inducing diuresis to remove edema.

Fructus Rubi is warm in nature; Sweet, sour; Return kidney, urinary bladder channel.Cure mainly: kidney tonifying, controlling nocturnal emission with astringent drugs, reducing urination.For enuresis due to deficiency of the kidney, frequent micturition, impotence and premature ejaculation, emission and spermatorrhea." Bencao Jingshu ": " Fructus Rubi, its main QI invigorating person, says beneficial vital essence also.Kidney storing essence, kidney improving inspiration by invigorating kidney-QI, vital essence is sufficient, then body is from light, sends out in vain also.Respectful main tonify deficiency of reviving is absolutely continuous, reinforcing YIN-essence Jianyang, smoothing skin, peace and internal organs.The main man's kidney-jing deficiency of Zhen Quan exhausts, impotence, woman's food have son.Large bright main settling five organs, beneficial color, supports vital essence, long hair, Qiang Zhi.All get its kidney tonifying and add essence, the adopted ear of sweet acid convergence." " property of medicine opinion " carry its: " main man's kidney-jing deficiency exhausts, woman's food have son.Main impotence.″

(Radix Polygalae is conventional Chinese medicine to Radix Polygalae, be recorded in Shennong's Herbal the earliest, be classified as top grade, and be regarded as supporting life key medicine, derive from the dry root of milk wort Radix Polygalae Polygala tenuifolia Willd. and ovum leaf Radix Polygalae P.sibirica L..Warm in nature, bitter in the mouth, pungent, the function of have Fructus Alpiniae Oxyphyllae of calming the nerves, eliminate the phlegm, subsiding a swelling, the insomnia and dreamful sleep caused for disarmony between the heart and kidney, forgetful palpitation with fear, staring spells, expectoration is not well, sore swollen toxin, breast swell and pain.Shennong's Herbal: main cough with dyspnea damaging the spleen and stomach, tonifying for the deficiency, except pathogen, sharp nine orifices, Fructus Alpiniae Oxyphyllae is intelligent, normal hearing and eyesight, does not forget, strong will times power.

Caulis Polygoni Multiflori, latin name: medical material Caulis Polygoni Multiflori former Plant-Polygonum Polygonum multiflorumThunb. effect: nourish heart, calm the nerves, dredging collateral, dispel the wind, cure mainly: control insomnia, impairment caused by overstrain, hyperhidrosis, blood deficiency general pain, carbuncle, scrofula, wind skin ulcer scabies.Meridian distribution of property and flavor: sweet micro-hardship is flat.Compatibility of drugs: complex acid Semen Ziziphi Spinosae, grows the heart-yin, mind calming god; Join the Radix Rehmanniae, nourishing YIN of nourishing blood; Join Radix Asparagi, Radix Ophiopogonis, clear asthenic fire, nourish heart the moon; Join Rhizoma Et Radix Notopterygii, Radix Angelicae Pubescentis, expelling wind and dampness, Shu Li joint.Have a sleepless night for fidgets due to deficiency.Caulis Polygoni Multiflori has nourishing blood to tranquillize the mind effect, is main for the insomnia caused by deficiency of YIN insufficiency of blood, often coordinates with Cortex Albiziae mutual reinforcement between, also can be equal to Semen Ziziphi Spinosae, Semen Platycladi, Radix Polygalae and use.

Cortex Lycii is cold in nature, sweet in the mouth.Function cures mainly: removing heat from blood removes and steams, lung heat clearing pathogenic fire reducing.For deficiency of YIN hectic fever, hectic fever due to YIN-deficiency night sweat, cough due to lung-heat, spitting of blood, epistaxis.Heat clearing away, removing heat from blood.Control asthenia hectic fever night sweat, lung-heat cough with asthma, spit blood, epistaxis, stranguria with blood, quenches one's thirst, hypertension, carbuncle, malignant boil." herbal classic ": " pathogen in main five, hankers quenching one's thirst, general arthralgia." " not Lu ": " main air wets, lower breast side of body gas, and exopathogenic heat is had a headache, and mends internal injury great Lao and to hiss suction, hard muscle, reinforcing YIN-essence, sharp intestine and small intestine, resistance to cold and summer-heat.″

Fructus Ligustri Lucidi is used for the poor vision of the hepatic and renal YIN deficiency, visual deterioration, early whitening of beard and hair, soreness of waist tinnitus and fever due to yin deficiency etc.This product energy tonifying liver kidney yin, but the efficacy of a drug is gentle, slowly must get effect.Control poor vision, often join Radix Rehmanniae Preparata, Fructus Lycii is equal to use, controls early whitening of beard and hair, and often join the same use of Radix Clematidis, fever due to yin deficiency, often joins Cortex Lycii.The hepatic and renal YIN deficiency, Light-headedness, seminal emission tinnitus, soreness of the waist and knees, early whitening of beard and hair, coronary heart disease, hyperlipemia, hypertension, chronic hepatitis.The protective effect research of liver is thought, Fructus Ligustri Lucidi contains oleanolic acid, to rat skin lower injection, serum glutamic pyruvic transminase can be reduced, there is obvious protective effect to the hepatic injury of Carbon Tetrachloride Induced, and can liver cell regeneration be promoted. oleanolic acid also can make triglyceride accumulation minimizing in liver, hepatocellular degeneration necrosis obviously alleviates, glycogen accumulation increases, serum γglobulin declines; Ultrastructure visible hepatocyte mitochondrial swelling and endosomal vesicle degeneration alleviate, hepatic tissue interstitial inflammation habituation.

Poria cum Radix Pini is the Chinese medicine product of plant Poria; For Polyporaceae crouches the sclerotium of hole platymiscium Poria [(Poria cocos Schw.) Wolf], original kind is rudimentary plant, is the fungus colonized on pinaster.Medicinal effects is the white portion entertaining Radix Pini massonianae in the middle of dry sclerotia body.Nature and flavor are sweet, light flat.There are the functions such as eliminating dampness by diuresis, spleen invigorating, mind calming.For phlegm retention, edema, dysuria, have loose bowels, cardiopalmus, dizzy.Sweet in the mouth, light, property are put down, and are used as medicine and have the function of promoting diuresis to eliminate damp pathogen, strengthening the spleen stomach function regulating, mind tranquilizing and the heart calming.Nature and flavor are sweet, light flat.There are the functions such as eliminating dampness by diuresis, spleen invigorating, mind calming.For phlegm retention, edema, dysuria, have loose bowels, cardiopalmus, dizzy.Cortex Sclerotii Poriae, inducing diuresis to remove edema, the diseases such as the edema that wets for water, for palpitation with fear of having a guilty conscience, forgetful, insomnia, infantile convulsion, dysuria." not Lu ": treat dizziness due to wind pathogen, wind be empty, five kinds of over strain, xerostomia.Spasmolytic is throbbed with fear, and is enraged more, forgetful.Happy Fructus Alpiniae Oxyphyllae, supports spirit.

Concha Margaritifera is the powder of shellfish nacreous layer, has suppressing the hyperactive liver and subsiding YANG; To calm the nerves arresting convulsion; Effect of liver heat removing and eyesight improving.Become, tremble with fear.Return liver, heart channel.Suppressing the hyperactive liver and subsiding YANG; To calm the nerves arresting convulsion; Liver heat removing and eyesight improving is investigated.Function cures mainly suppressing the hyperactive liver, YANG hyperactivity suppressing, arresting convulsion, hemostasis.Control dizziness, tinnitus, cardiopalmus, insomnia, demented, infantile convulsion, spits blood, epistaxis, metrorrhagia." Chinese medicine voluminous dictionary ": liver nourishing is cloudy, clearing away liver-fire.Control demented infantile convulsion, dizziness, tinnitus, heart beating, pleuroperitoneum is swollen, women's heat in blood, metrorrhagia, and frightened the jerking of children's sends out convulsion." decoction pieces is newly joined ": suppressing the hyperactive liver and subsiding YANG, soul of calming the nerves, arresting convulsion epilepsy, the fullness-oppression in the epigastrium caused by heat that disappears, mist.

Record in a Semen Ziziphi Spinosae China medicine book Shennong's Herbal the earliest: " invigorating middle warmer the liver benefiting, hard muscles and bones help cloudy gas, and all the merit of Semen Ziziphi Spinosae also." record in Ming Dynasty's Li Shizhen (1518-1593 A.D.) Compendium of Material Medica, " insomnia due to insufficiency of the gallbladder-qi in ripe treatment, the disease of excessive thirst sweating due to debility for Semen Ziziphi Spinosae; Life is treated gallbladder-heat and is slept well, and all foot faints cloudy few positive medicine also.Effect nourishing the liver, mind calming, calms the nerves, arresting sweating.Control restlessness of asrhenia type and insomnia, palpitation with fear palpitation with a distress feeling, excessive thirst, sweating due to debility." herbal classic ": main trusted subordinate's cold and heat, pathogens agglomerating gas gathers, painful limbs, arthralgia chiefly caused by damp pathogen." not Lu ": main vexatious insomnia, umbilicus is pain up and down, and blood turns chronic diarrhea, sweating due to debility excessive thirst, invigorating middle warmer, the liver benefiting gas, and hard muscles and bones helps cloudy gas, makes us fertile strong." property of medicine opinion ": main muscles and bones wind, fries end and takes it as soup.Supplement to the Herbal: many lifes of sleeping make, and must not sleep and fry.Wang Haogu: control milk-livered sleeplessness, trembles with fear also, fries clothes; Control somnolence due to gallbladder-excess, heat also, use by life." book on Chinese herbal medicine converges and says ": astringing QI is calmed the nerves, and flourish muscle supports marrow, stomach function regulating activating the spleen." book on Chinese herbal medicine is new again ": suppressing the hyperactive liver is regulated the flow of vital energy, nourishing lung-YIN, warming middle-JIAO dampness removing, astringing QI hidroschesis, beneficial will is breathed out surely, hearing-improing and eyesight improving.Pharmacological action:. calm, syngignoscism: oral or lumbar injection all behaves with composure and somnolence Semen Ziziphi Spinosae decoct to rat, no matter daytime or night, Semen Ziziphi Spinosae all can show above-mentioned effect.Calm index when white mice is oral is 1.95, shows synergism with barbiturate, and after Semen Ziziphi Spinosae is used continuously 6 days, animal sleep can shoal, and namely decreased duration produces toleration, but drug withdrawal can disappear after 1 week.

Radix Paeoniae Rubra enters liver, spleen channel, nourishing blood to suppress the hyperactive liver, pain relieving in slow, and yin fluid astringing receives antiperspirant.Control the breast abdomen pain over the hypochondriac region, dysentery is suffered from abdominal pain, and spontaneous sweating, fever due to yin deficiency, menoxenia, metrorrhagia, leukorrhagia, married woman's blood closes obstructed, repercussive blood, can lose pus, beneficial woman's blood." Japan hanako materia medica ": control wind, mend labor, all diseases of main woman, and antenatal rear all diseases, Tong Yueshui, brings down a fever, relieving restlessness, QI invigorating, the capable hot disease in sky, pestilence miasma, mania and irritation, and married woman's blood is transported, and hemorrhoidal hamorrhage, rushes down blood, hemorrhoid complicated by anal fistula.Carbuncle on the back, skin ulcer scabies, headache, improving eyesight, conjunctival congestion exerts meat.The many QI invigorating of redness of the skin or complexion person, white person controls blood.

Fructus Gardeniae: another name Yellow Fructus Gardeniae, Fructus Gardeniae, white toad are the fruits of Maguireothamnus speciosus Fructus Gardeniae.At present, the fruit of Fructus Gardeniae is Chinese medicine, belongs to the 1st batch of medicine-food two-purpose resource that Ministry of Public Health is promulgated, has the effects such as hepatoprotective, function of gallbladder promoting, blood pressure lowering, calmness, hemostasis, detumescence.The diseases such as treatment icterohepatitis, bruise, hypertension, diabetes are usually used at tcm clinical practice.Calentura is vexed.This product bitter cold is fallen clearly, can rush down clearly three burnt pathogenic fire, eliminating heart-fire and relieving restlessness, the key medicine that calentura is vexed in order to control, restlessness is not peaceful, can use same with Semen Sojae Preparatum, as Decoction of Capejasmine and Fermented Soybean treatise on Febrile Diseases; If join Radix Scutellariae, Rhizoma Coptidis, Cortex Phellodendri etc., available calentura fire-toxin of controlling is flourishing, three burnt all heat and see that high heat is irritated, unconsciousness and delirium person, as Rhizoma Coptidis toxic materials clearing away decoction " outer Fructus Gardeniae platform is secret to be wanted ".

Cortex Albiziae nature and flavor are sweet, flat.There is the merit of resolving depression and blood, mind calming, subduing inflammation.There is the effect controlling irritability, melancholy, insomnia, lung abscess, carbuncle, scrofula, injured bone.Cortex Albiziae is the bark of leguminous plant Herba Albiziae, Cortex Albiziae another name close confused skin, night platform skin, Herba Albiziae veneer, Cortex Albiziae has resolving depression of calming the nerves, the effect of eliminating carbuncle of invigorating blood circulation, Cortex Albiziae has the effect for the treatment of irritability, melancholy, sleeplessness, inside and outside carbuncle and ulcer, traumatic injury, gastritis sufferer is careful takes Cortex Albiziae, and wind heat spontaneous perspiration, diseases caused by exogenous pathogenic factor sleeplessness person taboo take Cortex Albiziae." herbal classic ": main settling five organs, and will, makes us happy carefree.Supplement to the Herbal: parasite killing.

Rhizoma Polygonati: Rhizoma Polygonati is used as medicine with rhizome.There is boosting qi and nourishing yin, spleen invigorating, lung moistening, kidney benefiting functions.Be used for the treatment of weakness of the spleen and stomach, fatigue and asthenia, xerostomia lack of appetite, deficiency of the lung cough caused by dryness, asthenia of essence and blood, interior-heat such as to be quenched one's thirst the disease.Very effective in cure for diabetes.Rhizoma Polygonati has blood pressure lowering, blood sugar lowering, blood fat reducing, atherosclerosis, and slow down aging and the effect such as antibacterial, polygonatum polysaccharide has Immunestimulatory effect.For lung-dryness syndrome due to deficiency of YIN, the few expectorant of dry cough, and the chronic cough chronic cough etc. of YIN-deficiency of the lung and kidney.For weakness of the spleen and stomach.Both tonifying spleen-yin, again strengthening the spleen gas, obtain dizzy, soreness of the waist and knees for deficiency of kidney essence, early whitening of beard and hair and quenching one's thirst.Rhizoma Polygonati strengthening bone and muscle, beneficial marrow, bleaches and sends out; The cough expectorant be suitable for caused by lack of lung-YIN is few, dry cough without phlegm, the diseases such as hemoptysis.

Radix Bupleuri clearind deficient heat Chinese medicine, comes off for cold, fever, alternate attack of chill and fever, malaria, stagnation of QI due to depression of the liver, sternal rib pain, proctoptosis, uterus, menoxenia.Return Liver Channel, gallbladder meridian.Function: evacuate and bring down a fever, yang invigorating soothing liver-QI.Cure mainly: cold, fever, alternate attack of chill and fever, malaria, stagnation of QI due to depression of the liver, sternal rib pain, proctoptosis, uterus comes off, menoxenia.Treatment viral hepatitis: injection of Radix Bupleuri (every 1ml is containing crude drug 2g) 10 ~ 20ml adds the quiet note of 50% Glucose Liquid or quiet of 5% Glucose Liquid 250 ~ 500ml, every day 1 time was for 10 times 1 course for the treatment of, treatment viral hepatitis 120 example, wherein acute case 97 example, effective percentage is 98.4%; Chronic cases 23 example, effective percentage is 100%, all has better effect to improving symptom, retraction liver spleen, recovery liver function and hepatitis B antigen negative conversion rate.

Radix Polygoni Multiflori Preparata: Chinese medicine Radix Polygoni Multiflori have Radix Polygoni Multiflori and Radix Polygoni Multiflori Preparata point, direct slicing is used as medicine for Radix Polygoni Multiflori, dries be used as medicine for Radix Polygoni Multiflori Preparata with Semen sojae atricolor liquor after mixing steaming.The function of the two is different: the removing toxic substances of Radix Polygoni Multiflori function, eliminating carbuncle, loosening bowel to relieve constipation, is usually used in treatment scrofula carbuncle sore, rubella pruritus, dryness of the intestine constipation; The invigorating the liver and kidney of Radix Polygoni Multiflori Preparata function, benefiting essence-blood, black beard and hair, bone and muscle strengthening, for blood deficiency and yellow complexion, vertigo and tinnitus, early whitening of beard and hair, soreness of the waist and knees, numb limbs and tense tendons, bleeding not during menses, chronic malaria body void etc.Radix Polygoni Multiflori Preparata of the present invention is the good merchantable brand of invigrating kidney.

Herba Epimedii is Chinese conventional Chinese medicine, has very high medical value, perennial herb, and YIN nourishing is taken a tonic or nourishing food to build up one's health, and tonifying YANG is kept fit.Bitter and puckery flavor, suitable insomniac uses.Herb hyoscine.Cure mainly impotence and premature ejaculation, pain in the low back and legs, numb limbs and tense tendons, hemiplegia, neurasthenia, forgetful, tinnitus, dizzy disease such as grade.Excessive sheep flower bud is Chinese conventional Chinese medicine.

Specific embodiment 1:

Annual 4 ~ June gathers when Fructus Mori fruit maturation, cleans, decontamination, dries or omits after steaming and dry.

Again the Fructus Mori dried are added water or ethanol extraction.

Again by all the other raw material Radix Polygalaes, Caulis Polygoni Multiflori, Cortex Lycii, Fructus Ligustri Lucidi, Poria cum Radix Pini, Concha Margaritifera, Fructus Rubi, Fructus Gardeniae, Radix Paeoniae Rubra, Radix Curcumae, Semen Ziziphi Spinosae, Cortex Albiziae, the Rhizoma Atractylodis Macrocephalae, Rhizoma Polygonati, Herba Leonuri, Radix Bupleuri, Radix Polygoni Multiflori Preparata, Herba Epimedii; To add water or ethanol is condensed into extracting solution.

The extracting solution of mixing Fructus Mori extracting solution and other raw materials, adds appropriate dextrin or Mel and is in harmonious proportion then ultraviolet radiation for sterilizing and disinfecting and bottles, directly drink during use.

Specific embodiment 2:

Annual 4 ~ June gathers when Fructus Mori fruit maturation, cleans, decontamination, dries or omits after steaming and dry, about 5000g; The Fructus Mori dried are put into 10 times of water gagings, heating and refluxing extraction 2 times, each 1 ~ 2 hour, 2 extracting solution are merged and leaves standstill; Again the Fructus Mori medicinal residues that above-mentioned ethanol extraction is crossed are added 10 times amount alcohol heating reflux and extract 2 times, each 1 ~ 2 hour, 2 extracting solution are merged and leaves standstill; Merged by above-mentioned two kinds of extracting solution, reducing pressure after reclaiming ethanol, it is concentrated to put into concentrator, and by gained concentrated solution adjustment proportion to 1.06, decompression recycling ethanol, obtains Fructus Mori extracting solution; By surplus stock Radix Polygalae 1500g, Caulis Polygoni Multiflori 1500g, Cortex Lycii 1500g, Fructus Ligustri Lucidi 1500g, Poria cum Radix Pini 1500g, Concha Margaritifera 1500g, Fructus Rubi 1500g, Fructus Gardeniae 1500g, Radix Paeoniae Rubra 1500g, Radix Curcumae 1500g, Semen Ziziphi Spinosae 1500g, Cortex Albiziae 1500g, Rhizoma Atractylodis Macrocephalae 1500g, Rhizoma Polygonati 1500g, Herba Leonuri 1500g, Radix Bupleuri 1500g, Radix Polygoni Multiflori Preparata 1500g, Herba Epimedii 1500g, clean roguing, dry, be ground into granule, soak 1 hour; Soaked crude drug is put in multi-function extractor and decocts with water secondary; Add up the water of medical material 10 times amount for the first time, decoct 1.5 ~ 2 hours, get decocting liquid, filter; Second time adds up the water of medical material 7 times amount, decocts 1 ~ 1.5 hour, gets twice decocting liquid mixing, filters; The filtrate of filtration inserted in economic benefits and social benefits vacuum concentrator, when being concentrated into 90 DEG C, relative density is the concentrated solution of 1.50, sets to 0 ~ 5 DEG C of deepfreezes 24 hours; Cold preservation liquid is added the filter aid kieselguhr of 0.3%, filter, filtrate is inserted in economic benefits and social benefits vacuum concentrator again, is concentrated into every 1ml containing 1g crude drug amount, adds dextrin and make granule.Mixed with other raw material extracting solution made by Fructus Mori extracting solution, add Mel and be in harmonious proportion, ultraviolet disinfection, packs and get final product again.

Specific embodiment 3:

Annual 4 ~ June gathers when Fructus Mori fruit maturation, clean, decontamination, dry or omit after steaming and dry, about 3500g, again by surplus stock Radix Polygalae 1000g, Caulis Polygoni Multiflori 1000g, Cortex Lycii 1000g, Fructus Ligustri Lucidi 1000g, Poria cum Radix Pini 1000g, Concha Margaritifera 1000g, Fructus Rubi 1000g, Fructus Gardeniae 1000g, Radix Paeoniae Rubra 1000g, Radix Curcumae 1000g, Semen Ziziphi Spinosae 1000g, Cortex Albiziae 1000g, Rhizoma Atractylodis Macrocephalae 1000g, Rhizoma Polygonati 1000g, Herba Leonuri 1000g, Radix Bupleuri 1000g, Radix Polygoni Multiflori Preparata 1000g, Herba Epimedii 1000g cleans roguing, all raw materials are put into 10 times amount ethanol, heating and refluxing extraction 2 times, each 1 ~ 2 hour, 2 extracting solution are merged and leaves standstill, again the raw material medicinal residues that above-mentioned ethanol extraction is crossed are added 10 times of water gaging heating and refluxing extraction 2 times, each 1 ~ 2 hour, 2 extracting solution are merged and leaves standstill, merged by above-mentioned two kinds of extracting solution, reducing pressure after reclaiming ethanol, it is concentrated to put into concentrator, by gained concentrated solution adjustment proportion to 1.25, merges twice decocting liquid, filters to get filtrate, the filtrate of filtration inserted in economic benefits and social benefits vacuum concentrator, when being concentrated into 90 DEG C, relative density is the concentrated solution of 1.50, sets to 0 ~ 5 DEG C of deepfreezes 24 hours, cold preservation liquid is added the filter aid kieselguhr of 0.3%, filter, filtrate is inserted in economic benefits and social benefits vacuum concentrator again, is concentrated into every 1ml containing 1g crude drug amount, adds dextrin and make granule.Mixed with other raw material extracting solution made by Fructus Mori extracting solution, add dextrin and be in harmonious proportion, ultraviolet disinfection, packs and get final product again.

Specific embodiment 4:

Annual 4 ~ June gathers when Fructus Mori fruit maturation, clean, decontamination, dry or omit after steaming and dry, about 3800g, again by surplus stock Radix Polygalae 1000g, Caulis Polygoni Multiflori 1100g, Cortex Lycii 1000g, Fructus Ligustri Lucidi 1100g, Poria cum Radix Pini 1200g, Concha Margaritifera 1200g, Fructus Rubi 1100g, Fructus Gardeniae 1000g, Radix Paeoniae Rubra 1200g, Radix Curcumae 1000g, Semen Ziziphi Spinosae 1000g, Cortex Albiziae 1100g, Rhizoma Atractylodis Macrocephalae 1000g, Rhizoma Polygonati 1200g, Herba Leonuri 1000g, Radix Bupleuri 1200g, Radix Polygoni Multiflori Preparata 1100g, Herba Epimedii 1200g cleans roguing, getting all crude drug steeps in water, soak more than 2 hours, add 8 times amount of crude drug gross weight respectively, the water extraction of 6 times amount 2 times, extraction time is 2 hours, 1 hour, merge 2 extracting solution, extracting solution is centrifugal by tubular type, obtains centrifugal liquid, and centrifugal liquid is crossed D 101 macroporous resin and adsorbed, and first washes remove impurity with water, then carries out eluting with the ethanol of 70%, collects eluent, concentrating under reduced pressure, and one-tenth extractum, for component becomes 1, the medicinal residues of crude drug said extracted crossed add ethanol, and add the ethanol warm macerating 2 times of 10 times amount of medicinal residues gross weight, 8 times amount respectively, the warm macerating time is 2 hours, 1.5 hours, and temperature is 85 DEG C, merges 2 warm macerating liquid, warm macerating liquid is evaporated to 1.25 extractum when density is 60 DEG C, and extractum is added the extraction into ethyl acetate secondary of its 4 times amount, extract has reclaimed ethyl acetate, becomes extractum, obtains component 2, above-mentioned two kinds of extracting solution are merged, when concentrating under reduced pressure relative density is 80 DEG C 1.35 filtrate, reclaim ethanol, above-mentioned two kinds of extracting solution are inserted in economic benefits and social benefits vacuum concentrator, when being concentrated into 90 DEG C, relative density is the concentrated solution of 1.60, sets to 0 ~ 5 DEG C of deepfreezes 24 hours, cold preservation liquid is added the filter aid kieselguhr of 0.3%, filter, filtrate is inserted in economic benefits and social benefits vacuum concentrator again, is concentrated into every 1ml containing 1g crude drug amount, and sterilize after adding dextrin mediation bottling.

Test example 1: acute toxicity test of the present invention

One, test material:

Animal: Kunming mouse, body weight 21-24g, male and female half and half, Shandong University's biology laboratory breeding.Medicine: oral liquid of the present invention (preparing by implementation method 2).

Two, method:

1, LD50 calculates: adopt improvement karber's method, mice is divided into 5 groups at random, often organize 10, male and female half and half, dissolve suppository, are made into Cmax, by the administration of mice maximum permission capacity, institute is followed successively by 18,14.4 to dosage by crude drug amount, 11.5,9.2,7.4 (g.kg-1), in animal fasting (can't help water) after 18 hours, administration at twice in one day (interval half an hour), each 0.5ml, observes animal dead situation.

2, maximum tolerated dose measures (MTD value): get mice 20, each 10 of male and female.Suppository adding distil water is dissolved, is made into maximum concentration, by the maximum tolerated dose of animal, feed device and can twitch to inject to fill with and be as the criterion.In animal fasting (can't help water) after 18 hours, administration at twice in one day (interval half an hour), each 0.5ml (every ml containing crude drug 0.36g), total dose is 18g crude drug/kg.d, 300 times of quite clinical adult 50Kg body weight consumption.Continuous Observation 7 days after administration.

Three, result of the test:

In LD50 calculates when giving mice with maximum acceptable concentration and maximum permission capacity (18g/Kg.d), have no dead mouse, namely LD50 is not measured, ask maximum tolerated dose only, in 7 day observation period, its appetite of animal, activity, hair color, the mental status etc. are all normal, grow normal, there are no death.Namely select the 300 times of doses being equivalent to clinical dosage, and have no adverse reaction, show that acute toxicity is minimum, MTD > 18g/Kg.d.

Test example 2: acute skin irritation test report of the present invention

1, experiment purpose: detect the present invention to the stimulation/corrosiveness of laboratory animal skin and intensity

2, materials and methods:

2.1. tested material: oral liquid of the present invention (obtaining by embodiment method 2)

2.2. laboratory animal: one grade of large ear white race rabbit, 10, female, body weight 2.1-2.3kg; Thered is provided by Affiliated Hospital of Qingdao University biology department Experimental Animal Center;

2.3. test method:

Its spinal column both sides, back are cut by hair in test by the healthy rabbits selecting skin intact 10 for first 24 hours, the left and right each about 4cm × 4cm of unhairing area.Get tested material 0.2ml during test to be applied on two layers of gauze of 2.5cm × 2.5cm, be applied to skin of unhairing surface, side, oilpaper covers, immobilization with adhesive tape.Opposite side skin is as blank.Close after 4 hours, dismantle, and go residual tested material with warm water cleaning, within 1,24 and 48 hour after removing tested material, observe and smear position dermoreaction, and mark.

3, experimental result: any erythema does not appear in laboratory animal skin, red and swollen, inflammation phenomenon.

4, brief summary: judge according to skin wound repair standards of grading in " disinfection technology standard " (1999), the present invention belongs to nonirritant to animal skin stimulus intensity.

Embodiment 3: long term toxicity test data

1, experimental technique

Random for Cavia porcellus part is become 4 groups, often organizes 15.In guinea pig back spinal column both sides, depilatory is evenly coated, make its scope about 40 square centimeters of losing hair or feathers.Clean depilatory, observe and often organize Cavia porcellus after 24 hours and be coated with oral liquid solution 0.2,0.4 of the present invention and 0.8ml respectively, respectively containing crude drug 92,184 and 368mg, another group is coated with the every twice-daily of solvent 0.8ml, continuous 30 days, observe the ordinary circumstance of Cavia porcellus, experiment terminates rear execution animal and carries out hematology, blood biochemical and pathological examination.

2, result

Above-mentioned three groups of medication Cavia porcellus trunk unhairing districts, having no local skin has edema, hyperemia, erythema, petechia and ulcer.Medication group and control animals chroma of hair, ingest, the matched group no significant difference such as extremity are movable, blood biochemistry checking, medication group and matched group are all in normal range.Histopathologic examination, tests each group of heart, liver, kidney and local skin and is showed no obvious pathological changes.Prompting, Chinese medicine oral liquid long-term prescription of the present invention is showed no obvious toxic action to local skin and whole body important organ.

Result of the test shows: oral liquid experiment safety of the present invention.This medicine is 1: 150 to group B streptococcus, staphylococcus aureus, colibacillary minimal inhibitory concentration, gonococcus is 1: 150, Candida albicans is 1: 300, it is 5 minutes to the time of killing during 1: 20 dilution factor, be 10 minutes when 1: 50, being 20 minutes when 1: 100, is 40 minutes when 1: 200, is 8 hours when 1: 400.

Pharmacological experiment:

Oral liquid of the present invention is the compound oral liquid be made up of medicines such as Fructus Mori, Radix Polygalae, Radix Polygoni Multiflori Preparata, Herba Epimedii, Herba Leonuri, Fructus Ligustri Lucidi, Radix Paeoniae Rubra, there is invigorating brain and relieving mental uneasiness, spermatogenesis mends marrow, effect of liver heat removing and eyesight improving benefiting QI and nourishing blood, for neurasthenia, insomnia, the disease such as forgetful, dizzy.Observe oral liquid of the present invention herein to monoamine neurotransmitter 5 one HT in underage mouse ability of learning and memory and brain, DA, NA, the impact of 5 one HIAA content.

1 test material

1.1 medicine oral liquid of the present invention, is prepared by the method for embodiment 2 by Pharmacy department of the court; Positive control drug, ANSHEN JIANNAO YE, Beijing Tongrentang Technology Development Co.ltd. Pharmaceutical Factory produces, lot number: 4152850.

1.2 animal Kunming mouse 11 ~ 15g, male and female half and half, purchased from Changchun Biological Products Institute, credit number: SCXK.2002.0001.

1.3 instrument Y. type water mazes; ES.180 electronic analytical balance, Shenyang Long Teng company produces; RF.540 type spectrofluorophotometer, Japanese Shimadzu Corporation produces.

1.4 reagent 5. hydroxytryptamine creatinine sulfates, China Drug Co.'s Solution on Chemical Reagents in Shanghai purchasing and supply station, lot number: 65-02.28, Switzerland's import subpackage, purity 99%; 5. hydroxyindole acetic acid, Sigma product, lot number: 60k1083, purity 98% ~ 100%; Potassium bitartrate norepinephrine, Shanghai Hefeng Pharmaceutical Co., Ltd. produces, lot number: 0205037,2mg/mL; Dopamine hydrochloride, is produced by Shanghai Hefeng Pharmaceutical Co., Ltd., lot number: 0204011,20mg/2mL; Other reagent is domestic analytical pure.

2 methods and result

Mice 80 is got on the impact (water maze) of underage mouse ability of learning and memory, is divided at random: blank group, ANSHEN JIANNAO YE 13mL/kg administration group, oral liquid group of the present invention 10.0,5.0,2.5mL/kg 3 dosed administration groups.Administration every day 1 time, in the 11d of first administration, successive administration 15d, starts that mice is placed in water maze and trains.Experiment adopts Y. type water labyrinth apparatus.This device divides long arm end and two branch galianconism ends.Left side galianconism end is equipped with platform, stays in part, have lighting above platform as mice.Three branch arm ends leave the opening of a 10cm, and mid portion black acrylic covers, as secret tunnel.Depth of water 10cm, water temperature (30 ± 2) DEG C, train 5d altogether, every animal trains 10 times every day, during training, the afterbody of mice is put into water lightly towards two forks in labyrinth, directly reaching in 15S with it has the platform of light source for correct response, otherwise is considered as mistake, record the number of times that every animal is made a mistake every day, and calculate 5d average achievement.Experimental data represents with-X ± S, compares by the sample 2.1 mean t method of inspection, the results are shown in Table 1 between group.

Table 1 oral liquid of the present invention is on the impact (x ± s, n=16) of underage mouse ability of learning and memory

Note: with blank group than * P < 0.05, * * P < 0.01, * * * P < 0.001, following table all with.

From table 1 result, 10.0mL/kg dosage group of the present invention, starts the errors number that just can reduce mice significantly in the 3d of training, and along with the prolongation of administration time, errors number reduces gradually, illustrates that it can strengthen ability of learning and memory in mice.

2.2 impacts on monoamine transmitter contents in underage mouse brain

Get mice 80, the same grouping administration, successive administration 15d, after last administration, 1h is by sacrifice of animal, gets cerebral tissue rapidly, puts into liquid nitrogen immediately and fills with freezing, take out homogenate in batches, by 5-HT, DA, NA in fluorescence spectrophotometry Mice brain tissues, the content of 5-HIAA, experimental data represents with x ± s, compares by the sample average t method of inspection, the results are shown in Table 2 between group.

Table 2 oral liquid of the present invention is on the impact (x ± s, n=16) of monoamine transmitter contents in underage mouse brain

From table 2 result, oral liquid of the present invention can increase 5-HT, DA, NA content in mouse brain tissue significantly.

2.3 get mice 80 to the impact of underage mouse thymus, spleen, Weight-index, with 2.1 grouping administrations, after last administration, 1h is by sacrifice of animal, get thymus, spleen, adrenal gland weigh calculating organ index, experimental data represents with x ± s, compare between group by the sample average t method of inspection, the results are shown in Table 3.

Table 3 oral liquid of the present invention is correlated with on underage mouse the impact (x ± s, n=16) of organ index

From table 3 result, the present invention can increase Weight-index significantly.

3 discuss

Learning and memory is one of Premium Features of brain, is the important indicator weighing animal intelligent development, and learning and memory is realized by a series of physiological and biochemical procedure of nervous system.Monoamine neurotransmitter is to attention, and memory and respond have important regulating action, and it comprises NA, DA, 5-HT, 5-HIAA etc., NA, DA, 5-HT rich content in synapse, and it not only transmits relevant with synapse, and relevant with synapse maturation.The minimizing of NA, DA, 5-HT, 5-HIAA, must affect the balance of neurotransmitter in brain, cause intellectual damage and go down.Experimental result shows, ANSHEN BUNAO YE can strengthen the ability of learning and memory of underage mouse, also show that it can improve NA in animal brain simultaneously, DA, the content of 5-HT, ANSHEN BUNAO YE has been applied for many years on I narrow-necked earthen jar bed, effect obviously, determined curative effect, but be used for the treatment of forgetful mechanism of action to it and never carry out deep research, point out herein from the result that affects of monoamine neurotransmitter aspect in immature animal brain, ANSHEN BUNAO YE can by activating brain metabolism, increase multiple neurohumoral quantity in cerebral cortex, thus reach the function of hypermnesis.Experimental result also shows ANSHEN BUNAO YE can increase underage mouse adrenal gland weight, and administration group and matched group have notable difference, and this also may be relevant containing the kidney-reinforcing drug such as Fructus Mori, Herba Epimedii with its side.

Specific embodiment: the Qin, female, 27 years old.After readme traffic accident more than fright at night March.Usually like ponder thing, because his father suffers a car accident before half a year, blood streaming down one's cheeks at that time, witnesses rear that night and sleep disorder, after aggravate gradually, often cry and shout in bed, restlessness, almost must send out every night, greatly shaving one's head.After starting before February to occur to fall asleep, 1 hours sits up suddenly, and facial expression is terrified, seemingly see foreign body shape, with cardiopalmus, whole body sweating.Go to a doctor in the present here.Diagnosis red tongue, yellow and thin fur, thready and rapid pulse.Card belongs to hyperactivity of hesrt-fire and liver-fire, deficiency of YIN-blood.Control suitable suppressing the hyperactive liver mind calming, tranquilizing the mind.Fang Yong: Fructus Mori 30g, Radix Polygalae 12g, Caulis Polygoni Multiflori 11g, Cortex Lycii 11g, Fructus Ligustri Lucidi 11g, Poria cum Radix Pini 12g, Concha Margaritifera 13g, Fructus Rubi 11g, Fructus Gardeniae 12g, Radix Paeoniae Rubra 12g, Radix Curcumae 11g, Semen Ziziphi Spinosae 10g, Cortex Albiziae 11g, Rhizoma Atractylodis Macrocephalae 10g, Rhizoma Polygonati 12g, Herba Leonuri 12g, Radix Bupleuri 12g, Radix Polygoni Multiflori Preparata 11g, Herba Epimedii 13g, makes oral liquid, point early, middle and late 3 times oral.Serve on 15 days.Further consultation, tells fright at night attack times after taking medicine and subtracts greatly, and after falling asleep, oneself no longer sits up, and can fall asleep to daybreak, brain is also no longer given free rein to fancy, and almost no longer bad dream repeatedly.After front enters 15 days again, all diseases are all flat, sleep stable, do not recur always.

Claims

1. the Fructus Mori oral liquid of an Anshen Bunao, it is characterized in that, in described Anshen Bunao oral liquid, the parts by weight of various raw material comprise: Fructus Mori 10 ~ 50 parts, Radix Polygalae 10 ~ 20 parts, Caulis Polygoni Multiflori 10 ~ 20 parts, Cortex Lycii 10 ~ 20 parts, Fructus Ligustri Lucidi 10 ~ 20 parts, Poria cum Radix Pini 10 ~ 20 parts, Concha Margaritifera 10 ~ 20 parts, Fructus Rubi 10 ~ 20 parts, Fructus Gardeniae 10 ~ 20 parts, Radix Paeoniae Rubra 10 ~ 20 parts, Radix Curcumae 10 ~ 20 parts, Semen Ziziphi Spinosae 10 ~ 20 parts, Cortex Albiziae 10 ~ 20 parts, the Rhizoma Atractylodis Macrocephalae 10 ~ 20 parts, Rhizoma Polygonati 10 ~ 20 parts, Herba Leonuri 10 ~ 20 parts, Radix Bupleuri 10 ~ 20 parts, Radix Polygoni Multiflori Preparata 10 ~ 20 parts, Herba Epimedii 10 ~ 20 parts.

2. the Fructus Mori oral liquid of Anshen Bunao according to claim 1, it is characterized in that, in described Anshen Bunao oral liquid, the parts by weight of various raw material comprise: Fructus Mori 10 ~ 30 parts, Radix Polygalae 10 ~ 15 parts, Caulis Polygoni Multiflori 10 ~ 15 parts, Cortex Lycii 10 ~ 15 parts, Fructus Ligustri Lucidi 10 ~ 15 parts, Poria cum Radix Pini 10 ~ 15 parts, Concha Margaritifera 10 ~ 15 parts, Fructus Rubi 10 ~ 15 parts, Fructus Gardeniae 10 ~ 15 parts, Radix Paeoniae Rubra 10 ~ 15 parts, Radix Curcumae 10 ~ 20 parts, Semen Ziziphi Spinosae 10 ~ 20 parts, Cortex Albiziae 10 ~ 20 parts, the Rhizoma Atractylodis Macrocephalae 10 ~ 20 parts, Rhizoma Polygonati 10 ~ 20 parts, Herba Leonuri 10 ~ 20 parts, Radix Bupleuri 10 ~ 20 parts, Radix Polygoni Multiflori Preparata 10 ~ 20 parts, Herba Epimedii 10 ~ 20 parts.

3. the Fructus Mori oral liquid of Anshen Bunao according to claim 1, it is characterized in that, in described Anshen Bunao oral liquid, the parts by weight of various raw material comprise: Fructus Mori 10 ~ 50 parts, Radix Polygalae 10 ~ 20 parts, Caulis Polygoni Multiflori 10 ~ 20 parts, Cortex Lycii 10 ~ 20 parts, Fructus Ligustri Lucidi 10 ~ 20 parts, Poria cum Radix Pini 10 ~ 20 parts, Concha Margaritifera 10 ~ 20 parts, Fructus Rubi 10 ~ 20 parts, Fructus Gardeniae 10 ~ 20 parts, Radix Paeoniae Rubra 10 ~ 20 parts, Radix Curcumae 10 ~ 15 parts, Semen Ziziphi Spinosae 10 ~ 15 parts, Cortex Albiziae 10 ~ 15 parts, the Rhizoma Atractylodis Macrocephalae 10 ~ 15 parts, Rhizoma Polygonati 10 ~ 15 parts, Herba Leonuri 10 ~ 15 parts, Radix Bupleuri 10 ~ 15 parts, Radix Polygoni Multiflori Preparata 10 ~ 15 parts, Herba Epimedii 10 ~ 15 parts.

4. the preparation method of the Fructus Mori oral liquid of Anshen Bunao as described in any one of claims 1 to 3 item, it is characterized in that, the preparation process of the Fructus Mori oral liquid of described Anshen Bunao comprises following aspect:

A. the extraction of Fructus Mori;

B. the extraction of other crude drug;

5. the preparation method of looks improving and the skin nourishing Fructus Mori oral liquid according to claim 4, is characterized in that, in described step a, gather during annual fruit maturation in 4 ~ June, cleans, decontamination, dries or dries after slightly steaming.

6. the preparation method of the Fructus Mori oral liquid of Anshen Bunao according to claim 4, is characterized in that, in described step a, comprise further and the Fructus Mori dried are put into water, heating and refluxing extraction 2 times, each 1 ~ 2 hour, is merged by 2 extracting solution and leaves standstill; Again the Fructus Mori medicinal residues that above-mentioned water extraction is crossed are added alcohol heating reflux and extract 2 times, each 1 ~ 2 hour, 2 extracting solution are merged and leaves standstill; Merged by above-mentioned two kinds of extracting solution, decompression recycling ethanol, obtains Fructus Mori extracting solution.

7. the preparation method of the Fructus Mori oral liquid of Anshen Bunao according to claim 4, it is characterized in that, in described step b, other raw materials are cleaned roguing, dries, be ground into granule, steep in water, soak more than 2 hours, add the water extraction 2 times of 8 times amount of crude drug gross weight, 6 times amount respectively, extraction time is 2 hours, 1 hour; Merge 2 extracting solution, extracting solution is centrifugal by tubular type, obtains centrifugal liquid, and centrifugal liquid is crossed D 101 macroporous resin and adsorbed, and first washes remove impurity with water, then carries out eluting with the ethanol of 70%, collects eluent, concentrating under reduced pressure, one-tenth pasty state medicinal liquid.

8. the preparation method of the Fructus Mori oral liquid of Anshen Bunao according to claim 4, it is characterized in that, in described step c, above-mentioned two kinds of extracting solution are inserted in economic benefits and social benefits vacuum concentrator, when being concentrated into 90 DEG C, relative density is the concentrated solution of 1.25, sets to 0 ~ 5 DEG C of deepfreezes 24 hours; Cold preservation liquid is added the filter aid kieselguhr of 0.3%, filter, filtrate is inserted in economic benefits and social benefits vacuum concentrator again, is concentrated into every 1ml containing 1g crude drug amount, for subsequent use.

9. a preparation method for basis Fructus Mori oral liquid of Anshen Bunao as described in any one of claims 1 to 3 item, it is characterized in that, the preparation method of described oral liquid comprises further:

10. the preparation method of the Fructus Mori oral liquid of Anshen Bunao according to claim 9, it is characterized in that: in described step a, get all crude drug and steep in water, soak more than 2 hours, add the water extraction 2 times of 8 times amount of crude drug gross weight, 6 times amount respectively, extraction time is 2 hours, 1 hour; Merge 2 extracting solution, extracting solution is centrifugal by tubular type, obtains centrifugal liquid, and centrifugal liquid is crossed D 101 macroporous resin and adsorbed, and first washes remove impurity with water, then carries out eluting with the ethanol of 70%, collects eluent, concentrating under reduced pressure, and one-tenth extractum, for component becomes 1; In described step b, the medicinal residues of crude drug said extracted crossed add ethanol, and add the ethanol warm macerating 2 times of 10 times amount of medicinal residues gross weight, 8 times amount respectively, the warm macerating time is 2 hours, 1.5 hours, and temperature is 85 DEG C, merges 2 warm macerating liquid; Warm macerating liquid is evaporated to 1.25 extractum when density is 60 DEG C, and extractum is added the extraction into ethyl acetate secondary of its 4 times amount, extract has reclaimed ethyl acetate, becomes extractum, obtains component 2; In described step c, above-mentioned two kinds of extracting solution are merged, when concentrating under reduced pressure relative density is 80 DEG C 1.35 filtrate, reclaim ethanol, inserted in economic benefits and social benefits vacuum concentrator by above-mentioned two kinds of extracting solution, when being concentrated into 90 DEG C, relative density is the concentrated solution of 1.60, sets to 0 ~ 5 DEG C of deepfreezes 24 hours; Cold preservation liquid is added the filter aid kieselguhr of 0.3%, filter, filtrate is inserted in economic benefits and social benefits vacuum concentrator again, is concentrated into every 1ml containing 1g crude drug amount, and sterilize after adding dextrin mediation bottling.