CN105343516A - Selfheal oral liquid for treating liver-fire flaming and preparation method of oral liquid - Google Patents

Abstract

The invention discloses selfheal oral liquid for treating liver-fire flaming and swelling and pain of eyes, wherein the oral liquid consists of the following raw materials in parts by weight: 10-50 parts of selfheal, 10-20 parts of roasted semen celosiae, 10-20 parts of lycium sinensis, 10-20 parts of angelica sinensis, 10-20 parts of semen cassiae, 10-20 parts of glossy privet fruit, 10-20 parts of radix paeoniae alba, 10-20 parts of folium mori, 10-20 parts of sculellaria barbata, 10-20 parts of chrysanthemum morifolium, 10-20 parts of cortex moutan, 10-20 parts of rhizoma polygonati, 10-20 parts of centella asiatica, 10-20 parts of fructus forsythia, 10-20 parts of herba portulacae and 10-20 parts of dandelion. The oral liquid disclosed by the invention, when taken for a long time, is strongly targeted to the symptoms of dry and sore eyes, and blurred vision caused by liver-fire flaming and overuse of the eyes.

Description

A kind of Spica Prunellae oral liquid for the treatment of flaming up of liver-fire and preparation method thereof

Technical field

The present invention relates to the health product containing the liver heat removing and eyesight improving deriving from plant composition, particularly a kind of Spica Prunellae oral liquid and preparation method thereof for the treatment of flaming up of liver-fire, conjunctival congestion and swelling pain.

Background technology

Present city working clan generally uses computer office, usually feels that eyes are astringent, fuzzy.The dry and astringent just asthenopic one of eyes in fact, if you are often facing to computer or books, excessive eye can consume liver blood.A wound is had in " five kinds of impairments caused by overstrain " of Huangdi's Internal Classics: " impairment of blood by looking for a long time ".Here blood, what refer to is exactly liver blood.

Modern metropolitan cities working clan generally uses computer office, and tobacco and wine are immoderate, and night life is uncontrolled, stays up late, and these have all directly injured irritability.The traditional Chinese medical science thinks that the liver being in charge of the eyes: " liver-QI being connected with eyes, liver and then order can distinguish the five colors ".The pathological changes of liver often affects and order.As deficiency of liver-blood, then two dryness and uneasy feeling of the eye, blurring of vision or nyctalopia; Wind-heat of liver channel, visible conjunctival congestion is itched bitterly; Flaming up of liver-fire, visible keratoconjunctivitis; Excessive rising of liver-YANG, the then vertigo; Liver-wind stirring up internally, looks on visible order is oblique.

In fact, eyes and liver contact closely.Blood stored in the liver, namely liver has stored blood and regulates the function of blood volume, and liver opening at eye.The provisions that eyes are subject to blood just can look thing, and excessively uses eye, can make liver-blood deficiency, make binocular can not get the supply of nutrition, thus go out to lose face dry and astringent, see thing is fuzzy, nyctalopia etc.In addition, long-term sitting eye, except binocular blood supply insufficiency, cervical vertebra, lumbar vertebra also can produce strain, less than alleviation, also can must cause damage to liver.In this case, occur that eyes are tired, visual deterioration, even shallow complexion, dizzy symptom, just not strange yet.Watching for a long time and radiation, the eye health for computer gang has buried hidden danger.This kind of people needs hepatoprotective while attention eye hygiene to nourish blood.In diet, the animal liverss such as a little Hepar Sus domestica, Hepar Gallus domesticus are suitably eaten in suggestion, supplement the food that beef, Carassius auratus, Herba Spinaciae, Herba Capsellae etc. are rich in vitamin simultaneously; In Chinese medicine, Radix Angelicae Sinensis, the Radix Paeoniae Alba etc. can be enriched blood, and Flos Chrysanthemi, Fructus Lycii then have effect of improving eyesight, and the people through conventional eye can by its oral liquid in soaked generation.

Spica Prunellae is the dry fruit ear of labiate Spica Prunellae PrunellavulgarisL..All parts of the country are all produced, main product in Jiangsu, Zhejiang, Anhui, the ground such as Henan.Gather when summer, fruit ear was brownish red, removing impurity, dries.Raw use.Its property is pungent, bitter, cold.Return liver, gallbladder meridian.There is clearing away heat-fire, improving eyesight, effect of mass dissipating and swelling eliminating.

Treatment conjunctival congestion and swelling pain, dizzy, order pearl nyctalgia of having a headache.Spica Prunellae bitter cold master enters Liver Channel, and kind removing fire from the liver is with improving eyesight.With controlling flaming up of liver-fire, conjunctival congestion and swelling pain, it is medicinal to join Folium Mori, Flos Chrysanthemi, Semen Cassiae etc.Among this product liver heat removing and eyesight improving, nourishing the liver of slightly holding concurrently, joins Radix Angelicae Sinensis, Fructus Lycii, can be used for deficiecny of liver-YIN, ophthalmalgia, to night more particularly; Also can accessory stick up, Radix Glycyrrhizae use, as loose in Spica Prunellae (" Zhang Shi doctor is logical ").

Treatment scrofula, goiter.This product acrid in the mouth energy eliminating stagnation, bitter cold can expel the heat-evil, and it is medicinal to control pathogenic fire derived from stagnation of liver-QI often to join Bulbus Fritillariae Uninbracteatae, Fructus Forsythiae etc., and the scrofula of phlegm-fire cohesion, as Spica Prunellae soup (" Waike Zhengzong, Orthodox Manual of External Diseases "); With controlling goiter, then often join the use such as Thallus Laminariae (Thallus Eckloniae), Radix Scrophulariae, as Spica Prunellae cream (Golden Mirror of Medicine).

Treatment acute mastitis swells and ache.This product heat clearing away can go liver-fire, again can mass dissipating and swelling eliminating, can control acute mastitis and swell and ache, normal with Herba Taraxaci with (" book on Chinese herbal medicine converges and says ").If join Flos Lonicerae, pathopyretic ulcer can be controlled, as detoxicating pill (" blue or green capsule is secretly handed down ").

Shennong's Herbal carries it: " main cold and heat, scrofula, scrofula, head sore, broken disease.Loose wart stagnation of QI, swelling of the feet arthralgia chiefly caused by damp pathogen." Compendium of Material Medica carries it: " Spica Prunellae controls ophthalmalgia, and with Saccharum Sinensis Roxb. water logging one ight, get it and can separate interior-heat, slow liver-fire is also.Lou Quan is apt to cloud, Spica Prunellae control order pearl pain to night then notably, the magical effect, or with bitter cold snack made with traditional Chinese medicines instead notably, the also magical effect.Lid order pearl connects order originally, and liver system also, belongs to the warp of the moon of fainting.Night very and some bitter and cold medicines anti-notably, night is with cold also cloudy therefore also.The gas of the pure sun of summer withered official report, mends cloudy blood vessels of fainting, therefore controls this as god, with Yang Zhiyin also." " notes of Chongqing hall " sub " grass, micro-pungent and sweet, therefore among eliminating stagnation, have the merit with positive yin nourishing concurrently, namely what after losing blood, the person of being insomnia took sleeps, and its property is visible.Chen Jiuzhe is especially sweet, is used as medicine for victory.”

Pharmacological research

1. chemical composition: this product is containing organic acid such as the glycoside materials such as triterpene saponin, rutin, hyperin and ursolic acid, caffeic acid, free oleanolic acids; Containing the anthocyanin, d-Camphora, d-fenchone etc. of delphinidin, cyanidin in spica.

2. pharmacological action: this product decoct, water-leach liquor, alcohol-water leachate and alcohol leaching liquid all can obviously reduce laboratory animal blood pressure, and stem, leaf, fringe and herb all have hypotensive effect, but the effect of fringe is more obvious; This product water decoction-alcohol sedimentation liquid mouse peritoneal is injected, and has obvious antiinflammatory action; This product decoct all has certain inhibitory action to dysentery bacterium, Bacillus typhi, vibrio cholera, escherichia coli, Bacillus proteus, staphylococcus and Bacillus tuberculosis in vitro.

3. clinical research: combine and be used for the treatment of headache, dizzy traditionally, pharmacological research has hypotensive activity, and modern clinic, in order to Hypertension, helps blood pressure lowering; Get its mass dissipating and swelling eliminating and conventional control the person that belongs to liver-heat such as thyromegaly, lymphadenectasis, cyclomastopathy, hypertension; With Spica Prunellae, Herba Hedyotidis Diffusae, Rhizoma Imperatae, Radix Glycyrrhizae, Radix Isatidis, Radix Sophorae Tonkinensis, be decocted in water for oral dose, Treatment chronic Hepatitis B 50 example, cures 33 examples, and take a turn for the better 12 examples; With Spica Prunellae, Pseudobulbus Bletillae (Rhizoma Bletillae), grind to the powder altogether, Adeps Sus domestica adjusts cream to apply affected part, controls rhagadia manus et pedis 36 example, recovery from illness 34 example; Use Spica Prunellae 100g, decocting 2 times, foam washing both hands, treatment hand desquamation demonstrate,proves over one hundred example, all fully recovers.

Spica Prunellae compatibility Radix Angelicae Sinensis, Radix Paeoniae Alba Spica Prunellae liver heat removing scattered silt; Radix Angelicae Sinensis, the Radix Paeoniae Alba nourish blood and enrich blood. and three's compatibility separates stagnation of liver-QI with having, effect of nourishing the liver blood is used for the treatment of all diseases caused by hepatic depression and blood deficiency.

Spica Prunellae compatibility Flos Chrysanthemi Spica Prunellae clearing away liver-fire, suppressing the hyperactive liver sun; The cool liver of Flos Chrysanthemi heat clearing away. Papillary have liver heat removing, cool liver, suppressing the hyperactive liver merit is used for the treatment of flaming up of liver-fire, wind-heat of liver channel causes conjunctival congestion and swelling pain; Or the headache, dizzy that excessive rising of liver-YANG causes.

Spica Prunellae compatibility Thallus Laminariae (Thallus Eckloniae), Sargassum Spica Prunellae clearing away liver-fire Disperse hepatic depression; Thallus Laminariae (Thallus Eckloniae), the equal eliminating phlegm and softening indurated mass of Sargassum and diuretic.Three medicines have share eliminating stagnation of relieving inflammation or internal heat, effect of eliminating phlegm and softening indurated mass is used for the treatment of the caused scrofula of liver-fire expectorant knot.

Spica Prunellae compatibility Radix Scrophulariae, Fructus Forsythiae Spica Prunellae clearing away liver-fire and Disperse hepatic depression; Radix Scrophulariae quench eliminating stagnation; Fructus Forsythiae detoxicating and resolving stagnation of pathogens. three is with strengthening the scrofula being used for the treatment of phlegm-fire and tying mutually by the power of its eliminating stagnation of relieving inflammation or internal heat.

Various nourishing the liver liver-protection health-care product, medicine spread unchecked on the market now, but great majority are had a good apperance only, false propaganda on a grand scale, probably without any health care, but cause the huge injury of the person, therefore, the health product of the effective hepatoprotective of search of health have very real significance.Chinese medicine hepatoprotective has advantage that is nontoxic, that can be absorbed completely by organism, is the Main way that we study.Modern metropolitan cities working clan generally uses computer office watching for a long time, tobacco and wine are immoderate, the behavior of the injury livers such as night life is uncontrolled, stay up late, the provisions that eyes are subject to blood just can look thing, and excessively use eye, can liver-blood deficiency be made, make binocular can not get the supply of nutrition, thus go out to lose face dry and astringent, see thing is fuzzy, nyctalopia etc.In addition, long-term sitting eye, except binocular blood supply insufficiency, cervical vertebra, lumbar vertebra also can produce strain, less than alleviation, also can must cause damage to liver.If probably be difficult to adhere to by Drug therapy simple, carelessly use collyrium more can make eyes damaged, cause various oculopathy or blind danger, and it is cumbersome to take Chinese medicine, mouthfeel is not good, unsuitable long-term taking, and effect is also not necessarily desirable.

Summary of the invention

Technical problem to be solved by this invention is, city working clan generally uses computer office watching for a long time, tobacco and wine are immoderate, the behavior of the injury livers such as night life is uncontrolled, stay up late, the provisions that eyes are subject to blood just can look thing, and excessively uses eye, can make liver-blood deficiency, make binocular can not get the supply of nutrition, thus go out to lose face dry and astringent, see thing is fuzzy, nyctalopia etc.In addition, long-term sitting eye, except binocular blood supply insufficiency, cervical vertebra, lumbar vertebra also can produce strain, less than alleviation, also can must cause damage to liver.If probably be difficult to adhere to by Drug therapy simple, carelessly use collyrium more can make eyes damaged, cause various oculopathy or blind danger, and it is cumbersome to take Chinese medicine, mouthfeel is not good, unsuitable long-term taking, and effect is also not necessarily desirable.Chinese medicine hepatoprotective has advantage that is nontoxic, that can be absorbed completely by organism, it is the Main way that we study, the most mouthfeel of these medical materials that the present invention chooses is light, and drug effect is remarkable, can long-term taking, also do not affect Spica Prunellae taste, make people be easy to accept, more can reach tonify deficiency benefit vital essence preferably, dispelling pathogenic wind for improving eyesight, effect of enriching yin and nourishing kidney, specific aim is stronger.

For solving the problems of the technologies described above, the invention provides one and treat flaming up of liver-fire, the Spica Prunellae oral liquid of conjunctival congestion and swelling pain, Spica Prunellae oral liquid described in it comprises the raw material of following parts by weight: Spica Prunellae 10 ~ 50 parts, fry Semen Celosiae 10 ~ 20 parts, Fructus Lycii 10 ~ 20 parts, Radix Angelicae Sinensis 10 ~ 20 parts, Semen Cassiae 10 ~ 20 parts, Fructus Ligustri Lucidi 10 ~ 20 parts, the Radix Paeoniae Alba 10 ~ 20 parts, 10 ~ 20 parts, Folium Mori, Herba Scutellariae Barbatae 10 ~ 20 parts, Flos Chrysanthemi 10 ~ 20 parts, Cortex Moutan 10 ~ 20 parts, Rhizoma Polygonati 10 ~ 20 parts, Herba Centellae 10 ~ 20 parts, Fructus Forsythiae 10 ~ 20 parts, Herba Portulacae 10 ~ 20 parts, Herba Taraxaci 10 ~ 20 parts.

The Spica Prunellae oral liquid of described treatment flaming up of liver-fire, conjunctival congestion and swelling pain, the raw material of following parts by weight can be comprised: Spica Prunellae 10 ~ 30 parts in Spica Prunellae oral liquid described in it, fry Semen Celosiae 10 ~ 15 parts, Fructus Lycii 10 ~ 15 parts, Radix Angelicae Sinensis 10 ~ 15 parts, Semen Cassiae 10 ~ 15 parts, Fructus Ligustri Lucidi 10 ~ 15 parts, the Radix Paeoniae Alba 10 ~ 15 parts, 10 ~ 15 parts, Folium Mori, Herba Scutellariae Barbatae 10 ~ 20 parts, Flos Chrysanthemi 10 ~ 20 parts, Cortex Moutan 10 ~ 20 parts, Rhizoma Polygonati 10 ~ 20 parts, Herba Centellae 10 ~ 20 parts, Fructus Forsythiae 10 ~ 20 parts, Herba Portulacae 10 ~ 20 parts, Herba Taraxaci 10 ~ 20 parts.

The Spica Prunellae oral liquid of described treatment flaming up of liver-fire, conjunctival congestion and swelling pain, the raw material of following parts by weight can also be comprised: Spica Prunellae 10 ~ 50 parts in Spica Prunellae oral liquid described in it, fry Semen Celosiae 10 ~ 20 parts, Fructus Lycii 10 ~ 20 parts, Radix Angelicae Sinensis 10 ~ 20 parts, Semen Cassiae 10 ~ 20 parts, Fructus Ligustri Lucidi 10 ~ 20 parts, the Radix Paeoniae Alba 10 ~ 20 parts, 10 ~ 20 parts, Folium Mori, Herba Scutellariae Barbatae 10 ~ 15 parts, Flos Chrysanthemi 10 ~ 15 parts, Cortex Moutan 10 ~ 15 parts, Rhizoma Polygonati 10 ~ 15 parts, Herba Centellae 10 ~ 15 parts, Fructus Forsythiae 10 ~ 15 parts, Herba Portulacae 10 ~ 15 parts, Herba Taraxaci 10 ~ 15 parts.

For solving the problems of the technologies described above, the present invention also provides a kind of preparation method of Spica Prunellae oral liquid for the treatment of flaming up of liver-fire, conjunctival congestion and swelling pain, and described in it, the preparation process of Spica Prunellae oral liquid comprises following aspect:

A. gather when Spica Prunellae fruit ear is brownish red when summer, removing impurity, dries, raw use;

B. the Spica Prunellae dried pulverizing, immersion ethanol are added extraction heat;

C. by other raw material pulverizing described, immersion ethanol extraction;

D. the medical filtration again surplus stock extracted, concentrated stand-by;

E. mixed with the Spica Prunellae extracting solution prepared by other raw material extracting solution concentrated, sterilizing is prepared into oral solutions.

In described step a, can between the 6-8 month, when Spica Prunellae fruit ear is brownish red, harvesting, dry in the sun, allow it parch.

In described step b, the Spica Prunellae roguing chopping that can parch, steeps in the ethanol of 5-10 times amount, is equipped with by medicated powder in lid stainless steel cask, add 70% ethanol, and quality is 0.8-1 times of coarse powder, and stir, moistening airtight placement more than 1 hour, makes abundant expansion; Drug sample after moistening expansion is mixed pine by filter plate gauze bag parcel again bottom percolator after paving does loose, then powder is contained with rustless steel spoon, uniform loading percolator, dress 10-12 cm thick, even by T-bar pressure, then press aforesaid operations, loading in layer, suitable pressurization, medicated powder must not load 2/3 eminence exceeding percolator; Medicated powder face upper cover rustless steel orifice plate fastens, and opens the baiting valve below percolator, and puts a container, then slowly add 70% ethanol; Air between medicated powder powder to be discharged, and have ethanol to flow out about about 20L, close baiting valve, cover cylinder of filtering, dipping 24 hours, then open baiting valve and carry out percolation, control percolation speed and be generally 1000g medical material outflow 2 ~ 3ml per minute, filtrate puts into liquid container, and pours ethanol time emptying into liquid container; Percolate is merged and leaves standstill; Filtrate above-mentioned steps obtained is inserted in economic benefits and social benefits vacuum concentrator, and when being concentrated into 90 DEG C, relative density is the concentrated solution of 1.05, sets to 0 ~ 5 DEG C of deepfreezes 24 hours; Cold preservation liquid is added the filter aid kieselguhr of 0.3%, filter, filtrate is inserted in economic benefits and social benefits vacuum concentrator again, is concentrated into every 1ml containing 0.1g crude drug amount.

In described step c, other raw materials can be got and clean and dry, in 70 DEG C of baking ovens, dry 8-12h, take out and be placed in cooling in drying baker, pulverized 80-100 mesh sieve with pulverizer; To be soaked the particulate matter that sieves in water 12 hours, then put into extraction pot heated and boiled 1 hour, filter, filtrate is for subsequent use; Filtering residue adds water, and second time heating, boils 45 minutes, and filter, filtrate is for subsequent use; Filtering residue adds water again, and heated and boiled half an hour third time, filter, heating is concentrated into paste, stand for standby use.

In described steps d, paste extract can be placed in 70-90% ethanol and soak 1-2 hour, in water-bath, be heated to 60 DEG C-70 DEG C, lixiviate 2h, every 10min stirs once, by filtered through gauze, 50-60% ethanol is added in residue, 60 DEG C-70 DEG C are continued lixiviate 2h, and every 10min stirs once, filtered through gauze, merge lixiviating solution, be condensed into pasty state filtrate, then insert in economic benefits and social benefits vacuum concentrator, stand-by after concentrated.

In described step e, the Spica Prunellae extracting solution made can be mixed with other raw material extracting solution, bottle after ultraviolet ray sterilization bactericidal.

For solving the problems of the technologies described above, the present invention also provides a kind of preparation method of Spica Prunellae oral liquid for the treatment of flaming up of liver-fire, conjunctival congestion and swelling pain, the preparation process of all the other raw materials can also be: get other raw materials and clean roguing, steep in the ethanol of 5-10 times amount, medicated powder is equipped with in lid stainless steel cask, add 70% ethanol, quality is 0.8-1 times of coarse powder, stir, moistening airtight placement more than 1 hour, makes abundant expansion; Drug sample after moistening expansion is mixed pine by filter plate gauze bag parcel again bottom percolator after paving does loose, then powder is contained with rustless steel spoon, uniform loading percolator, dress 10-12 cm thick, even by T-bar pressure, then press aforesaid operations, loading in layer, suitable pressurization, medicated powder must not load 2/3 eminence exceeding percolator; Medicated powder face upper cover rustless steel orifice plate fastens, and opens the baiting valve below percolator, and puts a container, then slowly add 70% ethanol; Air between medicated powder powder to be discharged, and have ethanol to flow out about about 20L, close baiting valve, cover cylinder of filtering, dipping 24 hours, then open baiting valve and carry out percolation, control percolation speed and be generally 1000g medical material outflow 2 ~ 3ml per minute, filtrate puts into liquid container, and pours ethanol time emptying into liquid container; Percolate is merged and leaves standstill; Filtrate above-mentioned steps obtained is inserted in economic benefits and social benefits vacuum concentrator, and when being concentrated into 90 DEG C, relative density is the concentrated solution of 1.05, sets to 0 ~ 5 DEG C of deepfreezes 24 hours; Cold preservation liquid is added the filter aid kieselguhr of 0.3%, filter, filtrate is inserted in economic benefits and social benefits vacuum concentrator again, is concentrated into every 1ml containing 0.1g crude drug amount.

The present invention is directed to city working clan and generally use computer office watching for a long time, tobacco and wine are immoderate, night life is uncontrolled, the behavior of liver of staying up late, excessively must injure less than alleviation etc. with eye, can cause damage to liver like this, tonify deficiency benefit vital essence of the present invention, dispelling pathogenic wind for improving eyesight, effect of nourishing the liver and kidney, and it is not good to solve Chinese medicine mouthfeel, frangible shortcoming, remains the nutrient substance of Spica Prunellae greatly, makes it not have work in-process to run off, on the make avoiding secondary pollution, is the health care oral liquid of green health environmental protection completely.The most mouthfeel of these medical materials that the present invention chooses is light, and drug effect is remarkable, can long-term taking, makes people be easy to accept, more can reach preferably, and the eyes caused flaming up of liver-fire, excess eye-using are especially dry astringent, and fuzzy viewing symptom specific aim is stronger.

Detailed description of the invention

The traditional Chinese medical science thinks that the liver being in charge of the eyes: " liver-QI being connected with eyes, liver and then order can distinguish the five colors ".The pathological changes of liver often affects and order.As deficiency of liver-blood, then two dryness and uneasy feeling of the eye, blurring of vision or nyctalopia; Wind-heat of liver channel, visible conjunctival congestion is itched bitterly; Flaming up of liver-fire, visible keratoconjunctivitis; Excessive rising of liver-YANG, the then vertigo; Liver-wind stirring up internally, looks on visible order is oblique.

Eyes and liver contact closely.Blood stored in the liver, namely liver has stored blood and regulates the function of blood volume, and liver opening at eye.The provisions that eyes are subject to blood just can look thing, and excessively uses eye, can make liver-blood deficiency, make binocular can not get the supply of nutrition, thus go out to lose face dry and astringent, see thing is fuzzy, nyctalopia etc.In addition, long-term sitting eye, except binocular blood supply insufficiency, cervical vertebra, lumbar vertebra also can produce strain, less than alleviation, also can must cause damage to liver.In this case, occur that eyes are tired, visual deterioration, even shallow complexion, dizzy symptom, just not strange yet.Watching for a long time and radiation, the eyes for computer gang are good for urine and have been buried hidden danger.

The triterpene saponin that it is aglycon that Spica Prunellae herb contains with oleanolic acid (Oleanolicacid).Still containing the glycoside material such as rutin (Rutin), hyperin (Hyperoside). also containing organic acid such as ursolic acid (Ursolicacid), caffeic acid (Caffeicacid) and free oleanolic acids. still containing inorganic salts such as vitamin B1, vitamin C, vitamin K, carotene, resin, amaroid, tannin, volatile oil, alkaloid and potassium chloride.

The anthocyanin of delphinidin (Delphinidin) and cyanidin (Cyanidin) is contained in spica, d-Camphora (d-Camphor), d-fenchone (d-Fenchone), ursolic acid, fruit ear is containing ursolic acid (ursolicacid), oleanolic acid (oleanolicacid), ursolic acid and oleanolic acid are the Saponin of main glycoside unit, daucosterol (daucosterol), β-Amyrin (β-mayrin) and its lignoceric acid (tetra-cosanicacid), 20 produce alkanoic acid (hexacosanicacid), the ester of octocosoic acid (octa-cosanicacid) and melissic acid (triacontanicacid).

Herb is containing acidity many grain Polysaccharides from Prunella vulgaris L (prunellin) of tool anti human immune deficiency virus (HIV), also containing oleanolic acid, ursolic acid, oleanolic acid is the Saponin of glycoside unit, globulariacitrin (rutin), hyperin (hyperoside), caffeic acid (caffeicacid), vitamin (vitamins) C, D, D carotene (carotene), tannin (tan-nin), and thing sense (alkaloids) and volatile oil, wherein containing levo-camphor (camphor), dextrorotation fenchone (fenchone).

Modern study

To the effect of cardiovascular system

Spica Prunellae decoct (concentration is 1: 10 ten thousand ~ 1: 100 ten thousand) can make Cor Leporis in place and isolated frog heart shrinkage amplitude increase, but concentration increase (1: 20 ~ 1: 50) then makes shrinkage amplitude diminish.

The water-leach liquor of Spica Prunellae, ethanol water-leach liquor and 30% ethanol extraction, to anesthetized animal lumbar injection, intravenous injection or the oral effect that all reduces blood pressure, intravenous injection hypotensive effect is obvious, oral administration then hypotensive effect is faint. and decoct (100mg/kg) is injected in anesthetized dog, significantly lasting hypotensive effect can be produced, but there is tachylaxis, after vagotomy, its hypotensive effect obviously weakens. to renal hypertension dog, after taking medicine continuously 2 weeks, blood pressure has moderate reduction, returns to again originally level after drug withdrawal.Spica Prunellae stem, leaf, fringe and herb all have hypotensive effect, and the effect of fringe is more weak.

Resisting pathogenic microbes effect experiment in vitro shows: Spica Prunellae decoct all has certain inhibitory action to dysentery bacterium, Bacillus typhi, vibrio cholera, escherichia coli, Bacillus proteus, bacillus pyocyaneus and staphylococcus, streptococcus and Bacillus tuberculosis.

Spica Prunellae can make the pulmonary lesion of the sick mice of experimental M alleviate to some extent.Spica Prunellae fresh goods is squeezed the juice and is all had inhibitory action to staphylococcus aureus, beta hemolytic streptococcus, escherichia coli, Bacillus typhi, dysentery bacterium, diphtheria corynebacterium, anthrax bacillus, bacillus pyocyaneus.

Water logging agent (1:4) is also had inhibitory action in various degree to permitting some common pathogenic dermatophytes such as blue achorion, Du's big belly microspore achorion difficult to understand in vitro.

Immunosuppressive action

Experiment shows: Spica Prunellae injection 6g (crude drug)/kg subcutaneous injection, can make Cavia porcellus, mice adrenal gland obviously increases, the obvious atrophy of thymus, spleen.Spica Prunellae water decoction-alcohol sedimentation liquid (1: 1) intramuscular injection 3g (crude drug)/kg, can make adult rat adrenal tissue weight increase, and Thymus and spleen weight saving, the wherein remarkable atrophy of thymus, compared with matched group, on average alleviates 63.4%.In addition, the organizational structure of adrenal cortex, thymus and spleen still can be made to change to some extent.

Spica Prunellae decoct (1: 50 ~ 1: 200) can make rabbit isolated uterine occur tetanic contraction.To isolated rabbit intestinal, high concentration can strengthen it and wriggle, and the most responsive with duodenum, ileum sensitivity is poor.Once had report, 100% Spica Prunellae decoct can suppress the growth of mice S180 and ehrlich carcinoma, but the anti-more not administration group of mortality rate is higher.

Spica Prunellae water decoction-alcohol sedimentation liquid all has inhibitory action to mouse knoting oil ear swelling and the swelling of rat yeast toes.

The mature seed that Semen Celosiae (Semencelosiae) is amaranthaceous plant annual herb Herba Celosiae argenteae.All there is wild or cultivation in most of China area.Semen Celosiae is one of conventional a herb of the traditional Chinese medical science, has effect of heat of dispeling the wind, clearing away liver-fire, nebula removing." herbal classic is met former ": " Semen Celosiae, controls wind heat order disease, same with Semen Cassiae merit.It controls wind scabies itching over the body, heat in skin, with the wind heat of the JUE YIN meridian invasion of vessels that can fall apart also." modern clinic also can be used for disease such as treatment hypertension etc.But deficiency of the liver order disease is unsuitable alone, platycoria, glaucoma patient prohibit clothes.

Radix Paeoniae Alba nourishing blood to suppress the hyperactive liver, pain relieving in slow, yin fluid astringing receives antiperspirant.Control the breast abdomen pain over the hypochondriac region, dysentery is suffered from abdominal pain, spontaneous sweating, fever due to yin deficiency, menoxenia, metrorrhagia, leukorrhagia." Japan hanako materia medica ": " control wind and mend consumptive disease, all diseases of main woman, and antenatal rear all diseases, Tong Yueshui, relieving restlessness of bringing down a fever, QI invigorating, controls the capable hot disease in sky, pestilence miasma mania and irritation, and married woman's blood is transported, and hemorrhoidal hamorrhage rushes down blood, hemorrhoid complicated by anal fistula carbuncle on the back, skin ulcer scabies, headache, improving eyesight, conjunctival congestion, bulbar conjunctiva polyp." Radix Paeoniae Alba extract raises hepatic injury caused by D-galactosamine and SGPT obvious antagonism, SGPT can be made to reduce, and make hepatocellular pathological changes and downright bad recover normal. the gross activity of the lactic acid dehydrogenase that the acute liver injury of rats that the ethanol extraction of the Radix Paeoniae Alba can make aflatoxin B1 cause shows and isozyme raises and is minimized. and white peony root's total glycoside can suppress carbon tetrachloride induced mice Plasma glutamate-pyruvate transaminase and lactic acid dehydrogenase to raise, and acid denaturation is bitten to liver organization, necrosis has certain antagonism. and someone thinks, hepatic injury caused by carbon tetrachloride destroys because of membrane structure generation peroxidation, glutamate pyruvate transaminase and lactic acid dehydrogenase in blood are raised, white peony root's total glycoside may have protective effect to hepatocyte injury, therefore transaminase can be made to reduce.

Fructus Ligustri Lucidi is used for the poor vision of the hepatic and renal YIN deficiency, visual deterioration, early whitening of beard and hair, soreness of waist tinnitus and fever due to yin deficiency etc.This product energy tonifying liver kidney yin, but the efficacy of a drug is gentle, slowly must get effect.Control poor vision, often join Radix Rehmanniae Preparata, Fructus Lycii is equal to use, controls early whitening of beard and hair, and often join the same use of Fructus Mori, fever due to yin deficiency, often joins Cortex Lycii.The hepatic and renal YIN deficiency, Light-headedness, seminal emission tinnitus, soreness of the waist and knees, early whitening of beard and hair, coronary heart disease, hyperlipemia, hypertension, chronic hepatitis.The protective effect research of liver is thought, Fructus Ligustri Lucidi contains oleanolic acid, to rat skin lower injection, serum glutamic pyruvic transminase can be reduced, there is obvious protective effect to the hepatic injury of Carbon Tetrachloride Induced, and can liver cell regeneration be promoted. oleanolic acid also can make triglyceride accumulation minimizing in liver, hepatocellular degeneration necrosis obviously alleviates, glycogen accumulation increases, serum γglobulin declines; Ultrastructure visible hepatocyte mitochondrial swelling and endosomal vesicle degeneration alleviate, hepatic tissue interstitial inflammation habituation.

Fructus Lycii is traditional rare Chinese medicine, is again a kind of tonic food, and being most suitable for arranges in pairs or groups with Ganoderma takes.In the list of 63 kinds of medicine-food two-purposes of Ministry of Public Health announcement, rank first.As Chinese medicine, the flat sweet in the mouth of Fructus Lycii property, has effect of nourishing the liver and kidney, replenishing vital essence to improve eyesight, lung moistening, arranges in pairs or groups can prevent all kinds of diseases and ailments with Ganoderma.Pass through chemical composition analysis, the fruit of Fructus Lycii detects containing nutritional labelings such as abundant carotene, vitamin C, Fructus Lycii proteoglycan, betanin, linoleic acid and ferrum, phosphorus, calcium, has tonify deficiency to calm the nerves, improving eyesight is dispeled the wind, the effect such as nourishing kidney lung moistening and hepatoprotective antitumor.Fructus Lycii contains the required nutrient substance of abundant carotene, multivitamin and the healthy eye such as calcium, ferrum, therefore has the merit of improving eyesight, is commonly called as " bright eye ".What ancient Chinese medicine doctor treatment deficiency of liver-blood, deficiency of kidney-YIN caused looks thing duskization and nyctalopia, usually uses Fructus Lycii.Further, Fructus Lycii is from the Book of Songs " combines in luxuriant Qi ", and just for medicine, the history in existing more than 3000 years so far, just has the alone Fructus Lycii of Jin Chaoge flood to smash juice from ancient times and drip order, the story for the treatment of ophthalmology illness.The name of Fructus Lycii, first appeared in Shennong's Herbal, is listed as top grade, very popular over the past thousands of years.

Folium Mori are dried leaves of moraceae plants Mulberry.Gather after first frost, removing impurity, drying and obtain, is a kind of wind-heat-dispersing medicinal, both oral administration, also can external application.It is cold in nature, sweet in the mouth, hardship, has effect of dispelling wind and heat pathogens, clearing away lung-heat and moistening for dryness, liver heat removing and eyesight improving, can treat anemopyretic cold, lung-heat type cough, dizziness headache, the disease that conjunctival congestion is dim-sighted.In modern times, doctor trained in Western medicine uses Folium Mori and Folium Mori biological preparation as the medicine improving diabetes and other various difficult miscellaneous diseases, thinks that its drug effect is very extensive.There are clearing away lung-heat and moistening for dryness, cough-relieving, reduce phlegm and internal heat, reduce phlegm, control night sweat; Tonifying liver, liver heat removing and eyesight improving, treatment dizziness and blurred vision, insomnia, elimination eye strain; Detumescence, purify the blood ' treatment dysentery, stomachache, fat-reducing, except beriberi, sharp large and small intestinal; Anti-stress, removing heat from blood, blood pressure lowering, blood fat reducing, prevention myocardial infarction, cerebral hemorrhage, dispel headache, long hair; Blood sugar lowering, anti-sugar are sick.

Flos Chrysanthemi is name little Tang Huang, chryanthemum parthenium also, is the special product in area, Tongxiang.Flos Chrysanthemi, the Flos Chrysanthemi in it and Anhui, the Flos Chrysanthemi in Henan is all that the tea that China is famous uses chrysanthemum.Flos Chrysanthemi in state-owned long cultivation history." Hang Bai Flos Chrysanthemi " one is to carrying with " Dragon Well tea well-known tea ".Ancient times once made tribute.Hang Bai Flos Chrysanthemi lobe is as white as polished jade, and pistil is yellow as proof gold.Very high medical value, effect of life lengthening, " mending agriculture book " carries: " the sweet temperature of Flos Chrysanthemi property, clothes are the most useful for a long time." Yuan Dynasty's " Amplification on Materia Medica addendum " says: " nourishing YIN of Flos Chrysanthemi energy ".Li Shizhen (1518-1593 A.D.) Compendium of Material Medica road: the sharp five pulse conditions of ZANG-organs of chrysanthemum energy, adjusts extremity, controls wind syndrome of head and vulcanize; Also record one section of mythology: " angle passes on a message, Kang Fengzi, all the becoming an immortal to take chrysanthemum of Zhu child ".Effect of visible Flos Chrysanthemi.Practice city's Flos Chrysanthemi, also can for oral liquid use, its color, smell and taste are not second to dragon well green tea.Often drink, can capillary resistance be strengthened, suppress the general character of blood capillary, play the effect that antiinflammatory is kept fit.

Cortex Moutan, has another name called Cortex Moutan, Chinese medicine name.For the dry root bark of ranunculaceae peony.Originate in the ground such as Anhui, Shandong.Autumn excavates root, and removing radicula, strips root bark, dry.Life is used or is fried and uses.Compendium of Material Medica: " nourishing YIN to lower pathogenic fire separates speckle poison, throat, diuresis stasis.Descendant specially controls fire mutually with yellow tiller, does not know that the merit of Cortex Moutan more wins also.Red colored person's profit, the person of spending in vain mends, and people also seldom realizes, and should distinguish it." modern study, contained paeonol and glucosides constituents in addition thereof all have antiinflammatory action; The methanolic extract of Cortex Moutan has suppression Platelet; Paeonol has the effects such as central inhibitory action and atherosclerosis, diuresis, antiulcer such as calmness, cooling, antipyretic, analgesia, spasmolytic.

Rhizoma Polygonati is used as medicine with rhizome.There is boosting qi and nourishing yin, spleen invigorating, lung moistening, kidney benefiting functions.Be used for the treatment of weakness of the spleen and stomach, fatigue and asthenia, xerostomia lack of appetite, deficiency of the lung cough caused by dryness, asthenia of essence and blood, interior-heat such as to be quenched one's thirst the disease.Very effective in cure for diabetes.Property sugariness put down, return spleen; Lung; Kidney channel is boosting qi and nourishing yin, spleen invigorating, lung moistening, the good medicine of kidney tonifying.Cure mainly deficiency of YIN chronic cough; Xeropulmonary cough; Insufficiency of the spleen weak; Lack of appetite xerostomia; Quench one's thirst; Deficiency of the kidney soreness of the waist and knees; Impotence and seminal emission; Tinnitus order is dark; Early whitening of beard and hair; Body void is won thin; Wind favus disease.Rhizoma Polygonati has blood pressure lowering, blood sugar lowering, blood fat reducing, atherosclerosis, and slow down aging and the effect such as antibacterial, polygonatum polysaccharide has Immunestimulatory effect.For lung-dryness syndrome due to deficiency of YIN, the few expectorant of dry cough, and the chronic cough chronic cough etc. of YIN-deficiency of the lung and kidney.For weakness of the spleen and stomach.Both tonifying spleen-yin, again strengthening the spleen gas, obtain dizzy, soreness of the waist and knees for deficiency of kidney essence, early whitening of beard and hair and quenching one's thirst.The good medicine of nourishing of good lung moistening tonification.

Semen Cassiae has liver heat removing and eyesight improving, loosening bowel to relieve constipation, the function of lowering blood-fat and reducing weight.Except containing except saccharide, protein, fat, also containing steroidal compounds, chrysophanol, emodin etc., also have the trace elements iron, zinc, manganese, copper, nickel, cobalt, molybdenum etc. of needed by human.Contained emodin, chrysophanol have human body relieving asthma, function of gallbladder promoting, protect the liver, hypotensive effect, and have necessarily antibacterial, anti-inflammatory effect.It is comparatively extensive that " China's book on Chinese herbal medicine " states its application, carries and say: " liver heat removing kidney tonifying, improving eyesight, diuretic relieving constipation.Cure mainly conjunctival congestion and swelling pain, photophobia tear be many, optic atrohic blindness, nyctalopia, headache and dizzy, poor vision, cirrhotic ascites, dysuria, habitual constipation.External treatment toxic swelling, tinea disease." " herbal classic ": " control optic atrohic blindness, the red tunica albuginea of the excessive skin of order, swelling ophthalmalgia, lacrimation, clothes benefit is with nothing left for a long time." " Bencao Jingshu ": " " not Lu " benefit is nontoxic with sweet being slightly cold of hardship for Semen Cassiae, its taste Cheng Ping.Become to obtain aqueous vapor, rusticly sweet, hardship can expel the heat-evil, flat conjunction gastric qi, and cold energy tonifying YIN expels the heat-evil, and the positive medicine of liver meridian man also.Also gallbladder kidney is entered.Liver opening at eye, gb 1 God Light belongs to kidney, therefore main optic atrohic blindness order is excessive, the red tunica albuginea of skin, swelling ophthalmalgia lacrimation." not Lu " holds concurrently and treats blue-purple lips." herbal classic " takes beneficial with nothing left person for a long time, tonifying YIN expels the heat-evil, caused by the gas of large invigorating the liver and kidney also." " book on Chinese herbal medicine is looked for the truth ": " Semen Cassiae, except wind heat radiation.Ordinary person's order tear is not received, and ophthalmalgia is more than, excessive in many genus wind heat, not up with hyperamization, controls when being expulsion; Can expel the heat-evil by this hardship, becoming can softening the hard mass, sweetly can enrich blood, and the thin air supporting of power, can rise loose ailment said due to cold or exposure again, therefore receives tear pain relieving key medicine for controlling order.And can rest the head on to control wind syndrome of head, but these clothes too, search wind to very, anti-evil of catching the wind, therefore must close with Fructus Tribuli, Flos Chrysanthemi, Fructus Lycii, the Radix Rehmanniae, Fructus Ligustri Lucidi reality, Fructus Sophorae, Flos Eriocauli mutually for subsidizing, then merit more wins.

Herba Portulacae Grandiflorae (formal name used at school: ScutellariabarbataD.Don) all herbal medicine, has heat-clearing and toxic substances removing, blood circulation promoting and blood stasis dispelling, reducing swelling and alleviating pain, the function such as anticancer.Sour in the mouth cold in nature, herb is containing compositions such as multivitamin, trace element and aminoacid.There is removing pathogenic heat from blood and toxic substance from the body, eliminating stasis to stop pain, effect of detumescence and clearing away heat-damp and promoting diuresis.Function cures mainly: have heat-clearing and toxic substances removing, blood circulation promoting and blood stasis dispelling, reducing swelling and alleviating pain, anticancer effect; Appendicitis curing, hepatitis, stomachache, early hepatocarcinoma, pulmonary carcinoma, cervical cancer, mastitis etc.; External is controlled pain furuncle and is treated, the diseases such as treating swelling and pain by traumatic injury.This medicine large usage quantity among the people." figure will planted by Guangxi medicine ": antiinflammatory, dissipating blood stasis stops blooding.Control traumatic injury and injure dysentery." Nanning City's medicine will ": reducing swelling and alleviating pain.Control traumatic injury knife injury and skin infection." Quanzhou book on Chinese herbal medicine ": dredging collateral, heat-clearing and toxic substances removing, dispel the wind loose blood, promoting the circulation of QI to induce diuresis, removing blood stasis with potent drugs pain relieving.Main stranguria with blood for oral administration, spits blood, epistaxis; Venom is controlled in external, carbuncle, furuncle, innominate toxic swelling.

Herba Taraxaci: dandelion herb is rich in vitamin A, vitamin C and potassium, also containing ferrum, calcium, vitamin B2, vitamin B1, magnesium, vitamin B6, folic acid and copper.Concrete constituent content mainly moisture, every 60 grams of dandelion herb leaf moisture contents 86%, 1.6 grams, protein, carbohydrate 5.3 grams, heat about has 108.8 kilojoules.Containing multiple health-nutrition compositions such as taraxol, taraxacin, choline, organic acid, inulin in Herba Taraxaci plant.Nature and flavor are sweet, micro-hardship, cold.Return liver, stomach warp.There are the effects such as diuresis, cathartic, jaundice eliminating subcutaneous ulcer, function of gallbladder promoting.Control pyretic toxicity, carbuncle, skin infection, abscess of internal organ, conjunctival congestion and swelling pain, damp and hot, the puckery pain of jaundice, dribbling urination, furuncle swelling toxin, acute mastitis, scrofula, toothache, conjunctival congestion, pharyngalgia, lung abscess, acute appendicitis, jaundice due to damp-heat, the puckery pain of pyretic stranguria.Control acute mastitis, lymphadenitis, scrofula, furuncle carbuncle toxin, acute conjunctivitis, cold, fever, acute tonsillitis, acute bronchitis, gastritis, hepatitis, cholecystitis, urinary tract infection etc.Herba Taraxaci is raw-eaten, stir-fry and eat, cook soup, is the plant of dietotherapeutic.

Herba Centellae Classification system Centellaasiatica (L.) Urban, has heat-clearing and toxic substances removing, inducing diuresis and reducing edema, nourishing, antiinflammatory, quelling effect.Deep skin cell can be stimulated to substitute.Herba Centellae can be compacted epidermis and corium coupling part, and skin can be made to limber up, and contributes to solving cutis laxa phenomenon, makes skin smooth flexible; Promote that collagen protein is formed in skin corium, makes fibrin regenerate, reconnects, skin is reached compact smooth effect.Also can help impaired organization healing and consolidation skin.Can to promote between epidermis with corium close is connected, the increase of adipose cell can be suppressed again, prevent hydroderma, occur fat.

Herba Portulacae (Classification system: PortulacaoleraceaL.) is Portulacaceae annual herb plant.Plump succulence, without hair, high 10 ~ 30cm, is born in the area without shade such as roadside, field and ruins, flower garden.All there is distribution domestic various places.This kind is medicine food dual purpose plant.Herb hyoscine, has clearing away heat-damp and promoting diuresis, removing toxic substances and promoting subsidence of swelling, antiinflammatory, quenches the thirst, diuresis; Seed improving eyesight.Modern study, Herba Portulacae is also containing abundant SL3 fatty acid and vitamin A sample material.SL3 fatty acid forms cell membrane, especially brain cell membrane and the necessary material of eye cell membrane; Vitamin A sample substance maintains epithelial tissue as skin, cornea and conjunctival normal function, participates in the synthesis of rhodopsin, strengthens retinal photoreceptor performance, also participates in many oxidizing processs in body.

Fructus Forsythiae is bitter, cool.Enter the heart, liver, gallbladder meridian.Heat clearing away, removing toxic substances, eliminating stagnation, detumescence.Control warm, erysipelas, macule, carbuncle and ulcer toxic swelling, scrofula, urine stranguria with urinary obstruction." herbal classic ": main cold and heat, Mus flaccidity, scrofula, carbuncle malignant boil, goiter, stagnation of pathogenic heat." property of medicine opinion ": main tonneau five types of stranguria, urinary obstruction, except heart man exopathogenic heat." Japan hanako materia medica ": logical small intestinal, evacuation of pus.Control furuncle, pain relieving, logical menstruation.

Specific embodiment 1:

Gather when 6-8 Spica Prunellae in middle of the month fruit ear is brownish red, removing impurity, dries, and raw use, Jiang Qipao enters alcohol extraction.

Again all the other raw materials are fried Semen Celosiae, Fructus Lycii, Radix Angelicae Sinensis, Semen Cassiae, Fructus Ligustri Lucidi, the Radix Paeoniae Alba, Folium Mori, Herba Scutellariae Barbatae, Flos Chrysanthemi, Cortex Moutan, Rhizoma Polygonati, Herba Centellae, Fructus Forsythiae, Herba Portulacae, Herba Taraxaci, pulverize, extraction, extract and make extracting solution.

Two kinds of extract mixing, ultraviolet radiation for sterilizing and disinfecting is bottled.

Specific embodiment 2:

Gather when 6-8 Spica Prunellae in middle of the month fruit ear is brownish red, removing impurity, dries, raw use, general 5000g, steeped in the ethanol of 5-10 times amount after roguing chopping, medicated powder is equipped with in lid stainless steel cask, adds 70% ethanol, quality is 0.8-1 times of coarse powder, stir, moistening airtight placement more than 1 hour, makes abundant expansion; Drug sample after moistening expansion is mixed pine by filter plate gauze bag parcel again bottom percolator after paving does loose, then powder is contained with rustless steel spoon, uniform loading percolator, dress 10-12 cm thick, even by T-bar pressure, then press aforesaid operations, loading in layer, suitable pressurization, medicated powder must not load 2/3 eminence exceeding percolator; Medicated powder face upper cover rustless steel orifice plate fastens, and opens the baiting valve below percolator, and puts a container, then slowly add 70% ethanol; Air between medicated powder powder to be discharged, and have ethanol to flow out about about 20L, close baiting valve, cover cylinder of filtering, dipping 24 hours, then open baiting valve and carry out percolation, control percolation speed and be generally 1000g medical material outflow 2 ~ 3ml per minute, filtrate puts into liquid container, and pours ethanol time emptying into liquid container; Percolate is merged and leaves standstill; Filtrate above-mentioned steps obtained is inserted in economic benefits and social benefits vacuum concentrator, and when being concentrated into 90 DEG C, relative density is the concentrated solution of 1.05, sets to 0 ~ 5 DEG C of deepfreezes 24 hours; Cold preservation liquid is added the filter aid kieselguhr of 0.3%, filter, filtrate is inserted in economic benefits and social benefits vacuum concentrator again, is concentrated into every 1ml containing 0.1g crude drug amount; Stand-by.

Get other raw materials again and fry Semen Celosiae 1500g, Fructus Lycii 1500g, Radix Angelicae Sinensis 1500g, Semen Cassiae 1500g, Fructus Ligustri Lucidi 1500g, Radix Paeoniae Alba 1500g, Folium Mori 1500g, Herba Scutellariae Barbatae 1500g, Flos Chrysanthemi 1500g, Cortex Moutan 1500g, Rhizoma Polygonati 1500g, Herba Centellae 1500g, Fructus Forsythiae 1500g, Herba Portulacae 1500g, Herba Taraxaci 1500g, cleans and dries, in 70 DEG C of baking ovens, dry 8-12h, take out and be placed in cooling in drying baker, pulverized 80-100 mesh sieve with pulverizer; To be soaked the particulate matter that sieves in water 12 hours, then put into extraction pot heated and boiled 1 hour, filter, filtrate is for subsequent use; Filtering residue adds water, and second time heating, boils 45 minutes, and filter, filtrate is for subsequent use; Filtering residue adds water again, and heated and boiled half an hour third time, filter, heating is concentrated into paste, stand for standby use.Paste extract is placed in 70-90% ethanol and soaks 1-2 hour, in water-bath, be heated to 60 DEG C-70 DEG C, lixiviate 2h, every 10min stirs once, by filtered through gauze, adds 50-60% ethanol in residue, 60 DEG C-70 DEG C are continued lixiviate 2h, and every 10min stirs once, filtered through gauze, merge lixiviating solution, be condensed into pasty state filtrate, then mix Spica Prunellae extracting solution, add Mel and be in harmonious proportion, ultraviolet disinfection, packaging bottling.

Specific embodiment 3:

Gather when 6-8 Spica Prunellae in middle of the month fruit ear is brownish red, removing impurity, dries, raw use, general 4500g.80-100 mesh sieve was pulverized with pulverizer; To be soaked the particulate matter that sieves in water 12 hours, then put into extraction pot heated and boiled 1 hour, filter, filtrate is for subsequent use; Filtering residue adds water, and second time heating, boils 45 minutes, and filter, filtrate is for subsequent use; Filtering residue adds water again, and heated and boiled half an hour third time, filter, heating is concentrated into paste, stand for standby use.Paste extract is placed in 70-90% ethanol and soaks 1-2 hour, in water-bath, be heated to 60 DEG C-70 DEG C, lixiviate 2h, every 10min stirs once, by filtered through gauze, add 50-60% ethanol in residue, 60 DEG C-70 DEG C are continued lixiviate 2h, and every 10min stirs once, filtered through gauze, merge lixiviating solution, be condensed into pasty state filtrate, stand-by.

Get other raw materials again and fry Semen Celosiae 1200g, Fructus Lycii 1100g, Radix Angelicae Sinensis 1200g, Semen Cassiae 1200g, Fructus Ligustri Lucidi 1100g, Radix Paeoniae Alba 1200g, Folium Mori 1100g, Herba Scutellariae Barbatae 1100g, Flos Chrysanthemi 1200g, Cortex Moutan 1100g, Rhizoma Polygonati 1100g, Herba Centellae 1200g, Fructus Forsythiae 1200g, Herba Portulacae 1100g, Herba Taraxaci 1100g, gets other raw materials and cleans roguing, steeps in the ethanol of 5-10 times amount, medicated powder is equipped with in lid stainless steel cask, add 70% ethanol, quality is 0.8-1 times of coarse powder, stirs, moistening airtight placement more than 1 hour, makes abundant expansion; Drug sample after moistening expansion is mixed pine by filter plate gauze bag parcel again bottom percolator after paving does loose, then powder is contained with rustless steel spoon, uniform loading percolator, dress 10-12 cm thick, even by T-bar pressure, then press aforesaid operations, loading in layer, suitable pressurization, medicated powder must not load 2/3 eminence exceeding percolator; Medicated powder face upper cover rustless steel orifice plate fastens, and opens the baiting valve below percolator, and puts a container, then slowly add 70% ethanol; Air between medicated powder powder to be discharged, and have ethanol to flow out about about 20L, close baiting valve, cover cylinder of filtering, dipping 24 hours, then open baiting valve and carry out percolation, control percolation speed and be generally 1000g medical material outflow 2 ~ 3ml per minute, filtrate puts into liquid container, and pours ethanol time emptying into liquid container; Percolate is merged and leaves standstill; Filtrate above-mentioned steps obtained is inserted in economic benefits and social benefits vacuum concentrator, and when being concentrated into 90 DEG C, relative density is the concentrated solution of 1.05, sets to 0 ~ 5 DEG C of deepfreezes 24 hours; Cold preservation liquid is added the filter aid kieselguhr of 0.3%, filter, filtrate is inserted in economic benefits and social benefits vacuum concentrator again, is concentrated into every 1ml containing 0.1g crude drug amount; Then mix Spica Prunellae extracting solution, add Mel and be in harmonious proportion, ultraviolet disinfection, packaging bottling.

Toxicology test

Long term toxicity test data

1, experimental technique

Random for Cavia porcellus part is become 4 groups, often organizes 15.In guinea pig back spinal column both sides, depilatory is evenly coated, make its scope about 40 square centimeters of losing hair or feathers.Clean depilatory, observe and often organize Cavia porcellus after 24 hours and be coated with oral liquid 0.2,0.4 of the present invention and 0.8ml respectively, respectively containing crude drug 92,184 and 368mg, another group is coated with the every twice-daily of solvent 0.8ml, continuous 30 days, observe the ordinary circumstance of Cavia porcellus, experiment terminates rear execution animal and carries out hematology, blood biochemical and pathological examination.

2, result

Above-mentioned three groups of medication Cavia porcellus trunk unhairing districts, having no local skin has edema, hyperemia, erythema, petechia and ulcer.Medication group and control animals chroma of hair, ingest, the matched group no significant difference such as extremity are movable, blood biochemistry checking, with of the present invention group with matched group all in normal range.Histopathologic examination, tests each group of heart, liver, kidney and local skin and is showed no obvious pathological changes.Prompting, Chinese medicine oral liquid life-time service of the present invention is showed no obvious toxic action to local skin and whole body important organ.Result of the test shows: Chinese medicine oral liquid experiment safety of the present invention.

Cumulative toxicity is tested:

Chinese medicine oral liquid of the present invention presses 7.69,19.18 and 43.21g crude drug/kg continuous use 15 weeks (1.0ml/100g body weight to mice, every day 2 times) and drug withdrawal after 3 weeks, result shows: the index such as hair, behavior, defecation, body weight, organ weights, hemogram, hepatic and renal function, blood glucose, blood fat of Chinese medicine oral liquid of the present invention to rat all has no significant effect, internal organs naked eyes do not find that difference change and histological indications show, medication 15 weeks and drug withdrawal are after 3 weeks, and each internal organs of rat are all without obviously changing.Illustrate that Chinese medicine oral liquid of the present invention is little to toxicity after rat long-term prescription, also there is no difference reaction after drug withdrawal, application safety.

Skin and the test of body internal stimulus:

1. getting rabbit and shave hair, is example with oral liquid of the present invention, carries out inducing and exciting contact test, viewing test rabbit after 24 hours and 48 hours, and all loseing skin has the phenomenon such as erythema and edema, with negative control group zero difference.Result shows, this patch is bordering on zero, namely without skin allergic reaction to dermoreaction intensity.

2. coloclysis cumulative toxicity test report in medicine body

Test method: select 20 healthy mices, female, each 10 of hero, dose design is first phase in every 5 days, each issue dosage is respectively 0.10LD50,0.15LD50,0.22LD50,0.34LD50,0.50LD50, the LD50 that is female, male mice of this sample be 5000m part/k part BW (five dosage is 500,750,1100,1700,2500m part/k part BW).Within every 5 days, weigh once, adjustment dosage, by 0.1ml/10 part BW per anum coloclysis.After 20 days, animal occurs without death condition, off-test, and by ascending-dose to tested material after 20 days, female, buck all occurs without death condition.

Pharmacological evaluation:

1 experiment material

1.1 medicine

Experimental drug: liver heat removing and eyesight improving oral liquid of the present invention, prepares by detailed description of the invention 2.Positive control drug: Qing Gan Capsule, Changchun Overseas Pharmaceutical Group Co, authentication code: the accurate word Z20050091 of traditional Chinese medicines.

1.2 animal Wistar rats, Kunming mouse, all use.

2 impacts on ALT, ET, NO content in hepatic injury mice serum

2.1 groupings: get 105 kunming mices and be divided into 6 groups at random, often organize 15, A group is Normal group; B group is model control group; C group is positive controls; D1 group is health oral liquor of the present invention (embodiment 2 is obtained) low dose group, and D2 group is dosage group in health care oral liquid of the present invention, and D3 group is health care oral liquid high dose group of the present invention;

2.2 modeling methods: the method establishment immunological liver injury in mice model adopting bacillus calmette-guerin vaccine and lipopolysaccharide associating.After each treated animal adaptability feeds 3d, except Normal group, every mice 1 tail vein injection bacillus calmette-guerin vaccine 5 × 107 viable bacterias, 12d intravenous injection lipopolysaccharide 7.5 μ g/ Mus after injection of BCG, put to death after 16h and draw materials, Normal group injects normal saline 2 times all in the same way.

2.3 administrations and process: the first day of intravenous injection bacillus calmette-guerin vaccine plays beginning gastric infusion, 1 time/d.Normal group and model control group: normal saline gavage, gavage amount is with the medication capacity of medication therapy groups; Positive control medicine treatment group: Qing Gan Capsule, 5.9g/kg dosed administration; The low 3.9g/kg of health care oral liquid treatment group of the present invention, middle 7.8g/kg, high 11.7g/kg dosed administration.Normal group is 16h after 12d injecting normal saline, and eyeball rear vein beard gets blood, and puts to death animal and get hepatic tissue censorship; All the other groups are 16h after intravenous injection lipopolysaccharide, and eyeball rear vein beard gets blood, and puts to death animal and get hepatic tissue censorship.

2.4 observation index and detection method: 1. detect glutamate pyruvate transaminase (ALT): adopt chemical colorimetry to measure.2. endogenous nitric oxide (N0) measures: adopt radioimmunology, take 0.5g liver and make 10% liver tissue homogenate, under 4 DEG C of conditions, 3000 turns/min, centrifugal 10min, gets supernatant.3. Endothelin (ET) detects: adopt radiation to exempt from method and directly measure blood plasma ET concentration.

Serum ALT respectively organized by table 1, the situation of change of ET, N0 compares

* compare with model group, * P < 0.05, * * P < 0.01; Δ compares with positive controls, Δ P < 0.05.

As can be seen from the above results, detecting qualified health care oral liquid of the present invention has significance to improve to ALT, ET, N0 content in hepatic injury mice serum, and has obvious protective effect to chronic hepatic injury, and comparing with positive control medicine has significant difference.

Clinical trial:

One, experimental subject: adopt oral liquid of the present invention altogether in 100 routine patient's experiments, each 50 people of experimental group matched group.Wherein man 52 example, female 48 example; The oldest person 55 years old, age reckling only has 18 years old.Major part sufferer is white collar, readme blurred vision, nyctalopia; Insomnia forgetfulness, deficiency of the yin of the liver, two dryness and uneasy feeling of the eye, visual deterioration; Flaming up of liver-fire, conjunctival congestion and swelling pain; Frequent eyes are dry astringent, stay up late, dinner party of drinking; And dim complexion, female's sufferer mostly has menoxenia, and pox is played in face, and mottle is serious.Disease is identical or similar, adopts commercially available liver heat removing and eyesight improving collyrium+Chinese medicine conditioning.

Therapeutic Method:

Usage and dosage: treatment group adopts Chinese medicine oral liquid of the present invention, its usage and consumption as follows:: every day 2 bottles.The course for the treatment of: be limited for one month.Matched group daily Chinese medicine (clinical according to individuality) adds dripping eyedrop, within one month, is limited.

The standard of curative effect evaluation

Cure: above symptom and sign improves completely, and patient is without any bad sensation, happy, in high spirits, and eyes are no longer dry astringent, blurred vision.Effective: sign is significantly improved, patient is substantially without bad sensation, happy, and spirit very well.Effective: sign obviously changes, random thoughts are slightly uncomfortable, but can accept, and mood is better, and energy is more abundant.Invalid: sign is without any change, and symptom, as front, feels depressed, and lethargy is depressed.Dim complexion, eyes or dry astringent.

The results are shown in Table 2 liang of group comparitive study

Group	Number	Recovery from illness	Take a turn for the better	Effectively	Invalid	Total effective rate
							Treatment group	50	39(78)	7(14)	2(4)	2(4)	96
Matched group	50	19(38)	12(24)	9(18)	10(20)	80

Treatment group successful is higher than matched group.

Show that this oral liquid replaces Chinese medicine, drink as daily, can mouthfeel be improved, also allow people not produce and detest psychology, Chinese medicine odor is no longer repelled.Computer office watching for a long time is generally used for city working clan, tobacco and wine are immoderate, uncontrolled, the excessive eye of night life must less than the behavior of injury livers such as alleviating, adopt tonify deficiency benefit vital essence, dispelling pathogenic wind for improving eyesight, the drug matching of nourishing the liver and kidney, and it is not good to solve Chinese medicine mouthfeel, can not the shortcoming of long-term taking, the present invention improves insomnia forgetfulness, deficiency of the yin of the liver, two dryness and uneasy feeling of the eye, visual deterioration; Flaming up of liver-fire, conjunctival congestion and swelling pain; Excess eye-using is caused to eyes are dry astringently has good health-care effect.

Claims (10)

1. treat the Spica Prunellae oral liquid of flaming up of liver-fire, conjunctival congestion and swelling pain for one kind, it is characterized in that, described Spica Prunellae oral liquid comprises the raw material of following parts by weight: Spica Prunellae 10 ~ 50 parts, fry Semen Celosiae 10 ~ 20 parts, Fructus Lycii 10 ~ 20 parts, Radix Angelicae Sinensis 10 ~ 20 parts, Semen Cassiae 10 ~ 20 parts, Fructus Ligustri Lucidi 10 ~ 20 parts, the Radix Paeoniae Alba 10 ~ 20 parts, 10 ~ 20 parts, Folium Mori, Herba Scutellariae Barbatae 10 ~ 20 parts, Flos Chrysanthemi 10 ~ 20 parts, Cortex Moutan 10 ~ 20 parts, Rhizoma Polygonati 10 ~ 20 parts, Herba Centellae 10 ~ 20 parts, Fructus Forsythiae 10 ~ 20 parts, Herba Portulacae 10 ~ 20 parts, Herba Taraxaci 10 ~ 20 parts.

2. treat flaming up of liver-fire according to claim 1, the Spica Prunellae oral liquid of conjunctival congestion and swelling pain, it is characterized in that, described Spica Prunellae oral liquid comprises the raw material of following parts by weight: Spica Prunellae 10 ~ 30 parts, fry Semen Celosiae 10 ~ 15 parts, Fructus Lycii 10 ~ 15 parts, Radix Angelicae Sinensis 10 ~ 15 parts, Semen Cassiae 10 ~ 15 parts, Fructus Ligustri Lucidi 10 ~ 15 parts, the Radix Paeoniae Alba 10 ~ 15 parts, 10 ~ 15 parts, Folium Mori, Herba Scutellariae Barbatae 10 ~ 20 parts, Flos Chrysanthemi 10 ~ 20 parts, Cortex Moutan 10 ~ 20 parts, Rhizoma Polygonati 10 ~ 20 parts, Herba Centellae 10 ~ 20 parts, Fructus Forsythiae 10 ~ 20 parts, Herba Portulacae 10 ~ 20 parts, Herba Taraxaci 10 ~ 20 parts.

3. treat flaming up of liver-fire according to claim 1, the Spica Prunellae oral liquid of conjunctival congestion and swelling pain, it is characterized in that, described Spica Prunellae oral liquid comprises the raw material of following parts by weight: Spica Prunellae 10 ~ 50 parts, fry Semen Celosiae 10 ~ 20 parts, Fructus Lycii 10 ~ 20 parts, Radix Angelicae Sinensis 10 ~ 20 parts, Semen Cassiae 10 ~ 20 parts, Fructus Ligustri Lucidi 10 ~ 20 parts, the Radix Paeoniae Alba 10 ~ 20 parts, 10 ~ 20 parts, Folium Mori, Herba Scutellariae Barbatae 10 ~ 15 parts, Flos Chrysanthemi 10 ~ 15 parts, Cortex Moutan 10 ~ 15 parts, Rhizoma Polygonati 10 ~ 15 parts, Herba Centellae 10 ~ 15 parts, Fructus Forsythiae 10 ~ 15 parts, Herba Portulacae 10 ~ 15 parts, Herba Taraxaci 10 ~ 15 parts.

4. as described in any one of claims 1 to 3 item, treat the preparation method of Spica Prunellae oral liquid for flaming up of liver-fire, conjunctival congestion and swelling pain, it is characterized in that, the preparation process of described Spica Prunellae oral liquid comprises following aspect:

A. gather when Spica Prunellae fruit ear is brownish red when summer, removing impurity, dries, raw use;

B. the Spica Prunellae dried pulverizing, immersion ethanol are added extraction heat;

C. by other raw material pulverizing described, immersion ethanol extraction;

D. the medical filtration again surplus stock extracted, concentrated stand-by;

E. mixed with the Spica Prunellae extracting solution prepared by other raw material extracting solution concentrated, sterilizing is prepared into oral solutions.

5. the preparation method of the Radix Salviae Miltiorrhizae leaf health care oral liquid of liver heat removing and eyesight improving according to claim 4, is characterized in that, in described step a, between the 6-8 month, when Spica Prunellae fruit ear is brownish red, plucks, dry in the sun, allows it parch.

6. the feature of the Radix Salviae Miltiorrhizae leaf health care oral liquid of liver heat removing and eyesight improving is according to claim 4, in described step b, by the Spica Prunellae roguing chopping parched, steep in the ethanol of 5-10 times amount, medicated powder is equipped with in lid stainless steel cask, add 70% ethanol, quality is 0.8-1 times of coarse powder, stir, moistening airtight placement more than 1 hour, makes abundant expansion; Drug sample after moistening expansion is mixed pine by filter plate gauze bag parcel again bottom percolator after paving does loose, then powder is contained with rustless steel spoon, uniform loading percolator, dress 10-12 cm thick, even by T-bar pressure, then press aforesaid operations, loading in layer, suitable pressurization, medicated powder must not load 2/3 eminence exceeding percolator; Medicated powder face upper cover rustless steel orifice plate fastens, and opens the baiting valve below percolator, and puts a container, then slowly add 70% ethanol; Air between medicated powder powder to be discharged, and have ethanol to flow out about about 20L, close baiting valve, cover cylinder of filtering, dipping 24 hours, then open baiting valve and carry out percolation, control percolation speed and be generally 1000g medical material outflow 2 ~ 3ml per minute, filtrate puts into liquid container, and pours ethanol time emptying into liquid container; Percolate is merged and leaves standstill; Filtrate above-mentioned steps obtained is inserted in economic benefits and social benefits vacuum concentrator, and when being concentrated into 90 DEG C, relative density is the concentrated solution of 1.05, sets to 0 ~ 5 DEG C of deepfreezes 24 hours; Cold preservation liquid is added the filter aid kieselguhr of 0.3%, filter, filtrate is inserted in economic benefits and social benefits vacuum concentrator again, is concentrated into every 1ml containing 0.1g crude drug amount.

7. the preparation method of the Radix Salviae Miltiorrhizae leaf health care oral liquid of liver heat removing and eyesight improving according to claim 4, is characterized in that, in described step c, get other raw materials clean dry, in 70 DEG C of baking ovens, dry 8-12h, taking-up is placed in cooling in drying baker, pulverizes 80-100 mesh sieve with pulverizer; To be soaked the particulate matter that sieves in water 12 hours, then put into extraction pot heated and boiled 1 hour, filter, filtrate is for subsequent use; Filtering residue adds water, and second time heating, boils 45 minutes, and filter, filtrate is for subsequent use; Filtering residue adds water again, and heated and boiled half an hour third time, filter, heating is concentrated into paste, stand for standby use.

8. the preparation method of the Radix Salviae Miltiorrhizae leaf health care oral liquid of liver heat removing and eyesight improving according to claim 4, it is characterized in that, in described steps d, paste extract is placed in 70-90% ethanol and soaks 1-2 hour, 60 DEG C-70 DEG C are heated in water-bath, lixiviate 2h, every 10min stirs once, by filtered through gauze, 50-60% ethanol is added in residue, 60 DEG C-70 DEG C are continued lixiviate 2h, and every 10min stirs once, filtered through gauze, merge lixiviating solution, be condensed into pasty state filtrate, then insert in economic benefits and social benefits vacuum concentrator, stand-by after concentrated.

9. the preparation method of the Radix Salviae Miltiorrhizae leaf health care oral liquid of liver heat removing and eyesight improving according to claim 4, is characterized in that, in described step e, mixed by the Spica Prunellae extracting solution made, bottle after ultraviolet ray sterilization bactericidal with other raw material extracting solution.

10. the preparation method of the Radix Salviae Miltiorrhizae leaf health care oral liquid of a liver heat removing and eyesight improving according to any one of claims 1 to 3, it is characterized in that, the preparation process of all the other raw materials can also be: get other raw materials and clean roguing, steep in the ethanol of 5-10 times amount, medicated powder is equipped with in lid stainless steel cask, add 70% ethanol, quality is 0.8-1 times of coarse powder, stir, moistening airtight placement more than 1 hour, makes abundant expansion; Drug sample after moistening expansion is mixed pine by filter plate gauze bag parcel again bottom percolator after paving does loose, then powder is contained with rustless steel spoon, uniform loading percolator, dress 10-12 cm thick, even by T-bar pressure, then press aforesaid operations, loading in layer, suitable pressurization, medicated powder must not load 2/3 eminence exceeding percolator; Medicated powder face upper cover rustless steel orifice plate fastens, and opens the baiting valve below percolator, and puts a container, then slowly add 70% ethanol; Air between medicated powder powder to be discharged, and have ethanol to flow out about about 20L, close baiting valve, cover cylinder of filtering, dipping 24 hours, then open baiting valve and carry out percolation, control percolation speed and be generally 1000g medical material outflow 2 ~ 3ml per minute, filtrate puts into liquid container, and pours ethanol time emptying into liquid container; Percolate is merged and leaves standstill; Filtrate above-mentioned steps obtained is inserted in economic benefits and social benefits vacuum concentrator, and when being concentrated into 90 DEG C, relative density is the concentrated solution of 1.05, sets to 0 ~ 5 DEG C of deepfreezes 24 hours; Cold preservation liquid is added the filter aid kieselguhr of 0.3%, filter, filtrate is inserted in economic benefits and social benefits vacuum concentrator again, is concentrated into every 1ml containing 0.1g crude drug amount.