CN104398986A

CN104398986A - Tranquilization and brain tonifying epimedium herb oral liquid and preparation method thereof

Info

Publication number: CN104398986A
Application number: CN201410699202.XA
Authority: CN
Inventors: 不公告发明人
Original assignee: Qingdao Shen Da New And High Technology Development Corp Ltd
Current assignee: Qingdao Shen Da New And High Technology Development Corp Ltd
Priority date: 2014-11-27
Filing date: 2014-11-27
Publication date: 2015-03-11

Abstract

The invention discloses tranquilization and brain tonifying epimedium herb oral liquid, which comprises the following raw medicaments in parts by weight: 10 to 50 parts of epimedium herb, 10 to 20 parts of yerbadetajo herb, 10 to 20 parts of schisandra chinensis, 10 to 20 parts of wolfiporia extensa, 10 to 20 parts of radix polygonum multiflorum preparata, 10 to 20 parts of glossy privet fruits, 10 to 20 parts of radix paeoniae alba, 10 to 20 parts of acorus gramineus, 10 to 20 parts of platycladi seeds, 10 to 20 parts of mulberries, 10 to 20 parts of nacres, 10 to 20 parts of polygala tenuifolia, 10 to 20 parts of cortex albiziae, 10 to 20 parts of ophiopogon japonicus, 10 to 20 parts of semen ziziphi spinosae, 10 to 20 parts of tuber fleeceflower stems, 10 to 20 parts of coptis chinensis, 10 to 20 parts of polygonatum odoratum, 10 to 20 parts of cortex moutan and 10 to 20 parts of curcuma aromatica. The tranquilization and brain tonifying epimedium herb oral liquid has the effects of engendering essence, supplementing marrow, reinforcing qi, nourishing blood, tonifying the brain and tranquilization, is used for dizziness, weakness, amnesia, insomnia and neurasthenia, which are caused by deficiency of kidney essence, qi and blood, is scientific in compatibility and high in efficacy, is a pure traditional Chinese medicinal preparation, does not have any toxic or side effect, has good mouth-feel, and can be taken for a long time; epimedium herb serves as a main material, and natural traditional Chinese medicine with the effects of tonifying the brain, tranquilization, promoting blood circulation, removing blood stasis, reinforcing qi, soothing the liver, relieving depression, calming the liver, suppressing yang, enriching yin, nourishing the kidney, clearing heat, removing toxicity and discharging fire is added, so that the metabolism of a human body can be regulated.

Description

Herba Epimedii oral liquid of a kind of Anshen Bunao and preparation method thereof

Technical field

The present invention relates to the medicinal preparation containing deriving from plant, animal or raw mineral materials medicine, particularly a kind of Herba Epimedii oral liquid and preparation method thereof of Anshen Bunao.

Background technology

The progress of society makes brain worker become the main force of society.Brain worker is the high-risk group of subhealth state, hunt for a job, promote, house, pay rise, sufferer, child education, every aspect life trival matters make modern worn out mentally and physically; According to an investigation display, the brain worker of nearly 90% is in sub-health state in various degree.This is because brain is human body the most accurate " instrument ", and brain worker bears the firm static work of single posture for a long time, makes muscle be in the state of constant tension, easily causes coagulation of QI-blood, cerebral blood supply insufficiency, thus bring out various disease.

Hunt for a job, promote, house, pay rise, sufferer, child education, every aspect life trival matters make modern worn out mentally and physically, sensation health unable to make ends meet.Add modern metropolitan cities working clan and generally use computer office, tobacco and wine are immoderate, and night life is uncontrolled, stay up late, and these have all directly injured Liver and kidney.Watching for a long time and radiation, the eye health for computer gang has buried hidden danger.This kind of people needs hepatoprotective while attention eye hygiene to nourish blood.The living and diet of modern people is too meticulous, and quantity of motion reduces, or eating and drinking too much at one meal, or overstrain, body metabolism function is failed gradually, causes visceral dysfunction, easily produce sleeping problems.Want to sleep well may become a lot of white collar, the extravagant hope of the gang that requires mental skill.

Social environmental pollution is serious, and life stress is large, makes a lot of people feel all not sleep well at urban life pressure; Add modern metropolitan cities working clan and generally use computer office watching for a long time, tobacco and wine are immoderate, the behavior of the injury Liver and kidney such as night life is uncontrolled, stay up late, the provisions that eyes are subject to blood just can look thing, and excessively use eye, can liver-blood deficiency be made, make binocular can not get the supply of nutrition, thus go out to lose face dry and astringent, see thing is fuzzy, nyctalopia etc.In addition, long-term sitting eye, except binocular blood supply insufficiency, cervical vertebra, lumbar vertebra also can produce strain, less than alleviation, also can must cause damage to liver.

Day by day flourishing due to economy, the continuous lifting of modern's living standard, cause people too much have deep love for enjoy, do not notice that the life style of oneself, behavioural habits, diet are had a liking for, thus the disease caused.Society with fast rhythm lives, the operating pressure of keen competition, serious threat is caused to the physical and mental health of modern, add that long-term diet is unreasonable, that is: smart grain replaces coarse grain completely, the pesticide remained in the absorption of a large amount of three-hypers (high heat, high protein, higher fatty acid), more than three (antiseptic is many, flavour enhancer is many, hormone many) food and food, chemical fertilizer stockpiling in vivo, add air, pollution of waterhead and quantity of motion less than normal, obesity and diabetes have just become the affluenza of city gang.Work hard, the difficulty in lasting nervous, worried, working and learning, mental work heavy for a long time, as with unhealthy emotion, more allows people feel dog-tired.

Herba Epimedii, property is worked hard, and taste temperature, returns Liver and kidney warp.Herb hyoscine.Cure mainly impotence and premature ejaculation, pain in the low back and legs, numb limbs and tense tendons, hemiplegia, neurasthenia, forgetful, tinnitus, dizzy disease such as grade.Herba Epimedii is Chinese conventional Chinese medicine.Primary treatment kidney invigorating and YANG supporting, expelling wind and removing dampness.Control sexual impotence erectile problem, hemiplegia is unsuccessful, dribbling urination, and muscles and bones contraction is anxious, and cough, optic atrohic blindness after being ill, toothache caused by fire of deficiency type, hemiplegia, weakness of the waist and knees, rheumatic arthralgia, extremity are meciless." herbal classic " carries it: main impotence is hindered absolutely, pains in the penis.Diuresis, physical strength profiting, Qiang Zhi." not Lu " carries it: hard muscles and bones.Disappear scrofula, red carbuncle; Skin ulcer is arranged at bottom, washes, and goes out worm." Japan hanako materia medica " carries it: control all cold wind consumptive disease, and mend waist knee joint, heart tonifying power, husband is exhausted, and sun does not rise, the exhausted cloudy loss of fecundity of woman, and muscles and bones contraction is anxious, and extremity are not appointed, and old man's dusk octogenarian, the middle age is forgetful." Elementary Medicine " carries it: the kidney invigorating is empty, supporing yang.Control hemiplegia hands and feet caused by paralysis, limbs skin is meciless.

Main containing flavone compound, lignanoid, alkaloid, volatilization wet goods. containing ceryl alcohol (Ceryl alcohol) in the plants such as Herba Epimedii, 1 alkane (Hentriacontane), plant sterol (Phytosterol), Palmic acid (Palmitic acid), stearic acid (Stearic acid), oleic acid (Oleic acid), linoleic acid (Linoleic acid), linolenic acid (Linolenic acid), ginnol (Bilobanol), magnoline (Magnoflorin), glucose (Glucose), fructose (Fructose), vitamin E etc. contain icariine (Icariin) in the leaf of Herba Epimedii and stem, icarisid (Icariside), des-o-methylicariine (des-O-Methylicariin), β-the 3,5,7-trihydroxy-2-(4-methoxy-phenyl)-8-(3-methyl-but-2-enyl)-chromen-4-one that anhydrates (β-anhy-Droicaritin), epimedoside (Epimedoside) A, B, C, D, E. in addition, Epimedium sagittatum is still containing isoquercitin (Iso-quercetin), Herba Epimedii-3-O-α-rhamnoside (Icaritin--3-O-α-rhamnoside), hyperin (Hyperin), Epimedium sagittatum glycoside (Sagittatoside) A, B, C, Epimedium sagittatum element (Sagittatin) A, B etc.

Various nourishing the liver hepatoprotective, the health product of Anshen Bunao, medicine spread unchecked on the market now, but great majority are had a good apperance only, false propaganda on a grand scale, probably without any health care, but cause the huge injury of the person, therefore, the health product of the effective hepatoprotective of search of health, Anshen Bunao have very real significance.Chinese medicine hepatoprotective, Anshen Bunao have advantage that is nontoxic, that can be absorbed completely by organism, are the Main way that we study.Chinese medicine also exists dual regulation, when central concentration is too high, may causes certain antagonism, and make drug effect influenced; During low dosage, possible drug level is too low, causes drug effect and does not show.And for the traditional Chinese medical science, no matter be from traditional Chinese medical science mechanism, the dosage form of institute's foundation or the formula components various aspects of medicine all do not have larger breakthrough, take mouthfeel not good, medicine is poor, take very inconvenient, the demand of various patient can not be met, the city life of modern high rhythm can not be met.

Summary of the invention

Technical problem to be solved by this invention is, now commercially various nourishing the liver hepatoprotective, the health product of Anshen Bunao, medicine spread unchecked, but great majority are had a good apperance only, false propaganda on a grand scale, probably without any health care, but cause the huge injury of the person, therefore, the health product of the effective hepatoprotective of search of health, Anshen Bunao have very real significance.Chinese medicine hepatoprotective, Anshen Bunao have advantage that is nontoxic, that can be absorbed completely by organism, are the Main way that we study.But Chinese medicine also exists dual regulation, when central concentration is too high, certain antagonism may be caused, and make drug effect influenced; During low dosage, possible drug level is too low, causes drug effect and does not show.Herba Epimedii, property is worked hard, and taste temperature, returns Liver and kidney warp.Herb hyoscine.Cure mainly impotence and premature ejaculation, pain in the low back and legs, numb limbs and tense tendons, hemiplegia, neurasthenia, forgetful, tinnitus, dizzy disease such as grade.Herba Epimedii is Chinese conventional Chinese medicine.Primary treatment kidney invigorating and YANG supporting, expelling wind and removing dampness.Control sexual impotence erectile problem, hemiplegia is unsuccessful, dribbling urination, and muscles and bones contraction is anxious, and cough, optic atrohic blindness after being ill, toothache caused by fire of deficiency type, hemiplegia, weakness of the waist and knees, rheumatic arthralgia, extremity are meciless.The present invention is the oral liquid be made up of medicines such as Herba Epimedii, Radix Polygalae, Radix Polygoni Multiflori Preparata, Radix Paeoniae Rubra, has invigorating brain and relieving mental uneasiness, and spermatogenesis mends marrow, effect of liver heat removing and eyesight improving benefiting QI and nourishing blood, for neurasthenia, insomnia, the disease such as forgetful, dizzy.

For solving the problems of the technologies described above, the invention provides a kind of Herba Epimedii oral liquid of Anshen Bunao, oral liquid described in it comprises the crude drug of following parts by weight: Herba Epimedii 10 ~ 50 parts, Herba Ecliptae 10 ~ 20 parts, Fructus Schisandrae Chinensis 10 ~ 20 parts, 10 ~ 20 parts, Poria, Radix Polygoni Multiflori Preparata 10 ~ 20 parts, Fructus Ligustri Lucidi 10 ~ 20 parts, the Radix Paeoniae Alba 10 ~ 20 parts, Rhizoma Acori Graminei 10 ~ 20 parts, Semen Platycladi 10 ~ 20 parts, Fructus Mori 10 ~ 20 parts, Concha Margaritifera 10 ~ 20 parts, Radix Polygalae 10 ~ 20 parts, Cortex Albiziae 10 ~ 20 parts, Radix Ophiopogonis 10 ~ 20 parts, Semen Ziziphi Spinosae 10 ~ 20 parts, Caulis Polygoni Multiflori 10 ~ 20 parts, Rhizoma Coptidis 10 ~ 20 parts, Rhizoma Polygonati Odorati 10 ~ 20 parts, Cortex Moutan 10 ~ 20 parts, Radix Curcumae 10 ~ 20 parts.

The Herba Epimedii oral liquid of described Anshen Bunao, oral liquid described in it can comprise the crude drug of following parts by weight: Herba Epimedii 10 ~ 30 parts, Herba Ecliptae 10 ~ 15 parts, Fructus Schisandrae Chinensis 10 ~ 15 parts, 10 ~ 15 parts, Poria, Radix Polygoni Multiflori Preparata 10 ~ 15 parts, Fructus Ligustri Lucidi 10 ~ 15 parts, the Radix Paeoniae Alba 10 ~ 15 parts, Rhizoma Acori Graminei 10 ~ 15 parts, Semen Platycladi 10 ~ 15 parts, Fructus Mori 10 ~ 15 parts, Concha Margaritifera 10 ~ 15 parts, Radix Polygalae 10 ~ 20 parts, Cortex Albiziae 10 ~ 20 parts, Radix Ophiopogonis 10 ~ 20 parts, Semen Ziziphi Spinosae 10 ~ 20 parts, Caulis Polygoni Multiflori 10 ~ 20 parts, Rhizoma Coptidis 10 ~ 20 parts, Rhizoma Polygonati Odorati 10 ~ 20 parts, Cortex Moutan 10 ~ 20 parts, Radix Curcumae 10 ~ 20 parts.

The Herba Epimedii oral liquid of described a kind of Anshen Bunao, oral liquid described in it can also comprise the crude drug of following parts by weight: Herba Epimedii 10 ~ 40 parts, Herba Ecliptae 10 ~ 20 parts, Fructus Schisandrae Chinensis 10 ~ 20 parts, 10 ~ 20 parts, Poria, Radix Polygoni Multiflori Preparata 10 ~ 20 parts, Fructus Ligustri Lucidi 10 ~ 20 parts, the Radix Paeoniae Alba 10 ~ 20 parts, Rhizoma Acori Graminei 10 ~ 20 parts, Semen Platycladi 10 ~ 20 parts, Fructus Mori 10 ~ 20 parts, Concha Margaritifera 10 ~ 20 parts, Radix Polygalae 10 ~ 15 parts, Cortex Albiziae 10 ~ 15 parts, Radix Ophiopogonis 10 ~ 15 parts, Semen Ziziphi Spinosae 10 ~ 15 parts, Caulis Polygoni Multiflori 10 ~ 15 parts, Rhizoma Coptidis 10 ~ 15 parts, Rhizoma Polygonati Odorati 10 ~ 15 parts, Cortex Moutan 10 ~ 15 parts, Radix Curcumae 10 ~ 15 parts.

For solving the problems of the technologies described above, the present invention also provides a kind of preparation method of Herba Epimedii oral liquid of Anshen Bunao, and described in it, the preparation process of oral liquid comprises:

The preparation of a, Herba Epimedii; For component 1

B, all the other crude drug heat are extracted 2 times, are component 2

C, above-mentioned two kinds of components to be merged, be condensed into pasty state add Mel or dextrin be in harmonious proportion after canned.

In described step a, Herba Epimedii can be put into the ethanol accounting for its quality 10 times amount, heating and refluxing extraction 2 times, each 1-2 hour, removes supernatant, merge extractive liquid, 100-110 order filters, then is the ultrafiltration post ultrafiltration of 6000-10000 through the molecular weight that dams, when ultrafiltrate concentrating under reduced pressure relative density is 80 DEG C 1.36 extractum, heating is concentrated into paste, stand for standby use; Again the medicinal residues that above-mentioned ethanol extraction is crossed are added the water heating and refluxing extraction 2 times accounting for its quality 10 times amount, remove supernatant, merge extractive liquid, 100-110 order filters, be the ultrafiltration post ultrafiltration of 6000-10000 again through the molecular weight that dams, when ultrafiltrate concentrating under reduced pressure relative density is 80 DEG C 1.36 extractum, heating be concentrated into paste, stand for standby use; Extract extractum by above-mentioned two kinds to merge, reducing pressure after reclaiming ethanol, it is concentrated to put into concentrator, and reduce pressure to obtain extractum.

In described step b, can get all the other crude drug and steep in water, soak more than 2 hours, add the water extraction 2 times of 8 times amount of crude drug medicine gross weight, 6 times amount respectively, extraction time is 2 hours, 1 hour; Merge 2 extracting solution, extracting solution is centrifugal by tubular type, obtains centrifugal liquid, and centrifugal liquid is crossed D 101 macroporous resin and adsorbed, and first washes remove impurity with water, then carries out eluting with the ethanol of 70%, collects eluent, concentrating under reduced pressure, one-tenth extractum.

In described step c, can extract extractum by above-mentioned two kinds and insert in economic benefits and social benefits vacuum concentrator, when being concentrated into 90 DEG C, relative density is the pasty state of 1.50, sets to 0 ~ 5 DEG C of deepfreezes 24 hours; Cold preservation liquid is added the filter aid kieselguhr of 0.3%, filter, filtrate is inserted in economic benefits and social benefits vacuum concentrator again, is concentrated into every 1ml containing 0.1g crude drug amount, then adds Mel or dextrin mediation bottling.

For solving the problems of the technologies described above, the present invention reoffers a kind of preparation method of Herba Epimedii oral liquid of Anshen Bunao, and the preparation method of described oral liquid comprises:

A, all crude drug are put into soak with ethanol, heat is extracted 2 times, is component 1;

B, again by the medicinal residues extracting in water 2 times that above-mentioned steps is extracted, be component 2;

C, finally by also 2-in-1 to component 1 and component, concentrated after stirring, put into Mel and be in harmonious proportion, ultraviolet virus killing bottling.

In described step a, all crude drug can be steeped 1-5 hour in the ethanol accounting for its quality 10 times amount, heating extraction 2 times, each 1-2 hour, removes supernatant, merge extractive liquid, 100-110 order filters, be the ultrafiltration post ultrafiltration of 6000-10000 again through the molecular weight that dams, when ultrafiltrate concentrating under reduced pressure relative density is 80 DEG C, the extractum of 1.36 is stand-by, is component 1

In described step b, the medicinal residues extracted can be added 10 times of water gagings, then put into extraction pot heated and boiled 1 hour, filter, filtrate is for subsequent use; Filtering residue adds water, and second time heating, boils 45 minutes, and filter, filtrate is for subsequent use; Filtering residue adds water again, heated and boiled half an hour third time, filters; Three filtrates be combined, by filtered through gauze, add 50-60% ethanol in residue, 60 DEG C-70 DEG C are continued lixiviate 2h, and every 10min stirs once, filtered through gauze, merge lixiviating solution, are condensed into pasty state, become component 2.

In described step c, two kinds of components can be merged, after reclaiming ethanol decompression put into concentrator concentrated time, when being concentrated into 90 DEG C, relative density is the concentrated solution of 1.50, sets to 0 ~ 5 DEG C of deepfreezes 24 hours; Cold preservation liquid is added the filter aid kieselguhr of 0.3%, filter, filtrate is inserted in economic benefits and social benefits vacuum concentrator again, is concentrated into every 1ml containing 0.1g crude drug amount, concentrated after stirring, and puts into Mel and is in harmonious proportion, ultraviolet virus killing bottling.

Advantageous Effects of the present invention is: spermatogenesis of the present invention mends marrow, and benefiting QI and nourishing blood, strong brain is calmed the nerves.For the dizziness caused by deficiency of kidney-essence, deficiency of both QI and blood, weak, forgetful, insomnia; Neurasthenia.The present invention take Herba Epimedii as major ingredient, add some invigorating brain and relieving mental uneasiness, blood circulation promoting and blood stasis dispelling, the natural Chinese medicine of inrigorating qi and promoting blood circulation, dispersing the stagnated live-QI to relieve the stagnation of QI, suppressing the hyperactive liver and subsiding YANG, enriching yin and nourishing kidney, heat-clearing and toxic substances removing, clearing away heat-fire, the metabolism function of human body can be regulated, compatibility science of the present invention, pure Chinese medicinal preparation, without any toxic and side effects, and instant effect, mouthfeel is good, can life-time service.

Detailed description of the invention

Brain worker is the high-risk group of subhealth state, and according to an investigation display, the brain worker of nearly 90% is in sub-health state in various degree.This is because brain is human body the most accurate " instrument ", and brain worker bears the firm static work of single posture for a long time, makes muscle be in the state of constant tension, easily causes coagulation of QI-blood, cerebral blood supply insufficiency, thus bring out various disease.

Rhizoma Acori Graminei removing dampness is whetted the appetite, eliminating phlegm of having one's ideas straightened out, refreshment Fructus Alpiniae Oxyphyllae.Not hungry for gastral cavity painful abdominal mass, a mouthful dysentery of keeping silent, coma epilepsy, forgetful deafness.Regulate the flow of vital energy, invigorate blood circulation, loose wind, dries.Control epilepsy, syncope due to accumulation of phlegm, calentura coma, forgetful, deafness with qi stagnation, ambition is unhappy, stomachache, stomachache, anemofrigid-damp arthralgia, swollen ulcer drug, traumatic injury.

Cortex Albiziae nature and flavor are sweet, flat.There is the merit of resolving depression and blood, mind calming, subduing inflammation.There is the effect controlling irritability, melancholy, insomnia, lung abscess, carbuncle, scrofula, injured bone.Cortex Albiziae is the bark of leguminous plant Herba Albiziae, Cortex Albiziae another name close confused skin, night platform skin, Herba Albiziae veneer, Cortex Albiziae has resolving depression of calming the nerves, the effect of eliminating carbuncle of invigorating blood circulation, Cortex Albiziae has the effect for the treatment of irritability, melancholy, sleeplessness, inside and outside carbuncle and ulcer, traumatic injury, gastritis sufferer is careful takes Cortex Albiziae, and wind heat spontaneous perspiration, diseases caused by exogenous pathogenic factor sleeplessness person taboo take Cortex Albiziae." herbal classic ": main settling five organs, and will, makes us happy carefree.Supplement to the Herbal: parasite killing.

Radix Curcumae this product is the dried root of zingiberaceous plant RADIX CURCUMAE Curcumawenyujin Y.H.Chen et C.Ling, Rhizoma Curcumae Longae Curcuma longaL..The above two practise title " RADIX CURCUMAE " and " Radix Curcuma " respectively, and all the other are practised by character difference and claim " osmanthus Radix Curcumae " or " green filament Radix Curcumae ".Winter, stem and leaf was excavated after withering, and removing silt and radicula, steam or boil to the saturating heart, dry.Nature and flavor with return through: pungent, bitter, cold.Return liver, the heart, lung meridian.Function cures mainly: promoting blood circulation and stopping pain, promoting QI circulation for relieving depression, and clear away heart-fire removing heat from blood, promoting the function of the gallbladder to alleviate jaundice.For amenorrhea dysmenorrhea, chest and abdomen swelling and pain, twinge, calentura coma, epilepsy is gone mad, jaundice dark coloured urine.

The Radix Paeoniae Alba is cool in nature, and bitter in the mouth acid, is slightly cold, has easing the affected liver of enriching blood, suppressing the hyperactive liver pain relieving.Yin fluid astringing receives the effects such as antiperspirant, is applicable to fever due to yin deficiency, menoxenia, the breast abdomen pain over the hypochondriac region, extremity contraction suddenly, dysentery stomachache, spontaneous sweating, metrorrhagia, the inferior disease of band.Paeoniflorin has antibacterial, antipyretic, antiinflammatory, increase coronary flow, improves the effect of myocardial nutritional flow, blood vessel dilating, antagonism acute myocardial ischemia, anticoagulant, calmness, analgesia, spasmolytic, antiulcer, adjustment blood glucose.Radix Paeoniae Alba decoct can suppress dysentery bacterium, streptococcus pneumoniae, escherichia coli, Bacillus typhi, Hemolytic streptococcus, bacillus pyocyaneus etc.The condition subject such as the blood deficiency deficiency of YIN, the breast abdomen pain over the hypochondriac region, pain in the hepatic region, cholecystitis, gallstone pain, dysentery stomachache, women's menalgia, spontaneous perspiration easy antiperspirant night sweat, sural spasm, spasm of the limbs pain, uneasy lower limb syndrome are suitable edible.

Rhizoma Polygonati Odorati contains Herba Albiziae glycoside, tannin, resolving stagnation for tranquilization, appetizing of regulating the flow of vital energy, activating collaterals to relieve pain, for irritability, melancholy insomnia.Control pent-up uncomfortable in chest, insomnia, forgetful, acute conjunctivitis, can settling five organs, and will, improving healthy complexion, have keep fit preferably, calm, the effect of calming the nerves, improve looks, be also treat neurasthenic good merchantable brand.Also have clearing away summer-heat, face-beautifying and spot-removing such as to relieve the effect of alcohol at the effect." herbal warp " is said: " Rhizoma Polygonati Odorati energy settling five organs, and will, improving healthy complexion." especially to anemia of pregnant woman, have keep fit preferably, calm, the effect of calming the nerves, improve looks, be also treat neurasthenic good merchantable brand.

Rhizoma Coptidis heat clearing and damp drying, eliminating fire and detoxication.For damp and hot feeling of fullness, vomiting acid regurgitation, dysentery, jaundice, unconsciousness due to high fever, hyperactivity of heart-fire, dysphoria and insomnia, heat in blood tells nosebleed, conjunctival congestion, and toothache, quenches one's thirst, carbuncle furuncle; External treatment eczema, eczema, auditory meatus is suppurated.It is burning hot that Rhizoma Coptidis (processed with wine) is apt to the clear part of the body cavity above the diaphragm housing the heart and lungs.For conjunctival congestion, aphtha.Prepared RHIZOMA COPTIDIS with rhizoma zingiberis recens juice clearing stomach stomach function regulating preventing or arresting vomiting.Tie mutually for cold and heat, damp and hot middle resistance, feeling of fullness is vomitted.Cornel Rhizoma Coptidis soothing liver-QI stomach function regulating preventing or arresting vomiting.For incoordination between the liver and stomach, vomiting acid regurgitation.

Fructus Ligustri Lucidi, sweet-bitter flavor, cool in nature, return Liver and kidney warp, there is nourishing the liver and kidney, merit that tonifying YIN nourishes blood.Modern medicine study proves, Fructus Ligustri Lucidi includes ursolic acid, mannitol, glucose, fatty wet goods, has heart tonifying, diuresis and hepatoprotective effect, the leukopenia quantity caused because of chemotherapy can be made to raise, and have inhibitory action to staphylococcus aureus, escherichia coli.The acid of oleanolic acid Fructus Ligustri Lucidi, nuezhenoside, ursolic acid, stearic acid, oleic acid, mannitol, betulin, phospholipid and saccharide is contained as Fructus Ligustri Lucidi polysaccharide, arabinose, glucose etc. in Fructus Ligustri Lucidi, also containing 15 seed amino acids, 11 kinds of trace element and volatilization wet goods.Research thinks that Fructus Ligustri Lucidi has blood sugar lowering and blood fat reducing and has study of anti-atherogenic effect, and antibacterial, antivirus action in addition, Fructus Ligustri Lucidi decoct all has inhibitory action to staphylococcus aureus, shigella flexneri, Bacillus typhi, bacillus pyocyaneus and escherichia coli.

Radix Polygalae (Radix Polygalae) is conventional Chinese medicine, be recorded in Shennong's Herbal the earliest, be classified as top grade, and be regarded as supporting life key medicine, derive from the dry root of milk wort Radix Polygalae Polygala tenuifoliaWilld. and ovum leaf Radix Polygalae P.sibiricaL..Warm in nature, bitter in the mouth, pungent, the function of have Fructus Alpiniae Oxyphyllae of calming the nerves, eliminate the phlegm, subsiding a swelling, the insomnia and dreamful sleep caused for disarmony between the heart and kidney, forgetful palpitation with fear, staring spells, expectoration is not well, sore swollen toxin, breast swell and pain.Shennong's Herbal: main cough with dyspnea damaging the spleen and stomach, tonifying for the deficiency, except pathogen, sharp nine orifices, Fructus Alpiniae Oxyphyllae is intelligent, normal hearing and eyesight, does not forget, strong will times power.

Caulis Polygoni Multiflori, latin name: medical material Caulis Polygoni Multiflori former Plant-Polygonum Polygonum multiflorumThunb. effect: nourish heart, calm the nerves, dredging collateral, dispel the wind, cure mainly: control insomnia, impairment caused by overstrain, hyperhidrosis, blood deficiency general pain, carbuncle, scrofula, wind skin ulcer scabies.Meridian distribution of property and flavor: sweet micro-hardship is flat.Compatibility of drugs: complex acid Semen Ziziphi Spinosae, grows the heart-yin, mind calming god; Join the Radix Rehmanniae, nourishing YIN of nourishing blood; Join Radix Asparagi, Radix Ophiopogonis, clear asthenic fire, nourish heart the moon; Join Rhizoma Et Radix Notopterygii, Radix Angelicae Pubescentis, expelling wind and dampness, Shu Li joint.Have a sleepless night for fidgets due to deficiency.Caulis Polygoni Multiflori has nourishing blood to tranquillize the mind effect, is main for the insomnia caused by deficiency of YIN insufficiency of blood, often coordinates with Cortex Albiziae mutual reinforcement between, also can be equal to Semen Ziziphi Spinosae, Semen Platycladi, Radix Polygalae and use.

Concha Margaritifera is the powder of shellfish nacreous layer, has suppressing the hyperactive liver and subsiding YANG; To calm the nerves arresting convulsion; Effect of liver heat removing and eyesight improving.Salty, cold.Return liver, heart channel.Suppressing the hyperactive liver and subsiding YANG; To calm the nerves arresting convulsion; Liver heat removing and eyesight improving is investigated.Function cures mainly suppressing the hyperactive liver, YANG hyperactivity suppressing, arresting convulsion, hemostasis.Control dizziness, tinnitus, cardiopalmus, insomnia, demented, infantile convulsion, spits blood, epistaxis, metrorrhagia." Chinese medicine voluminous dictionary ": liver nourishing is cloudy, clearing away liver-fire.Control demented infantile convulsion, dizziness, tinnitus, heart beating, pleuroperitoneum is swollen, women's heat in blood, metrorrhagia, and frightened the jerking of children's sends out convulsion." decoction pieces is newly joined ": suppressing the hyperactive liver and subsiding YANG, soul of calming the nerves, arresting convulsion epilepsy, the fullness-oppression in the epigastrium caused by heat that disappears, mist.

Record in a Semen Ziziphi Spinosae China medicine book Shennong's Herbal the earliest: " invigorating middle warmer the liver benefiting, hard muscles and bones help cloudy gas, and all the merit of Semen Ziziphi Spinosae also." record in Ming Dynasty's Li Shizhen (1518-1593 A.D.) Compendium of Material Medica, " insomnia due to insufficiency of the gallbladder-qi in ripe treatment, the disease of excessive thirst sweating due to debility for Semen Ziziphi Spinosae; Life is treated gallbladder-heat and is slept well, and all foot faints cloudy few positive medicine also.Effect nourishing the liver, mind calming, calms the nerves, arresting sweating.Control restlessness of asrhenia type and insomnia, palpitation with fear palpitation with a distress feeling, excessive thirst, sweating due to debility." herbal classic ": main trusted subordinate's cold and heat, pathogens agglomerating gas gathers, painful limbs, arthralgia chiefly caused by damp pathogen." not Lu ": main vexatious insomnia, umbilicus is pain up and down, and blood turns chronic diarrhea, sweating due to debility excessive thirst, invigorating middle warmer, the liver benefiting gas, and hard muscles and bones helps cloudy gas, makes us fertile strong." property of medicine opinion ": main muscles and bones wind, fries end and takes it as soup.Supplement to the Herbal: many lifes of sleeping make, and must not sleep and fry.Wang Haogu: control milk-livered sleeplessness, trembles with fear also, fries clothes; Control somnolence due to gallbladder-excess, heat also, use by life." book on Chinese herbal medicine converges and says ": astringing QI is calmed the nerves, and flourish muscle supports marrow, stomach function regulating activating the spleen." book on Chinese herbal medicine is new again ": suppressing the hyperactive liver is regulated the flow of vital energy, nourishing lung-YIN, warming middle-JIAO dampness removing, astringing QI hidroschesis, beneficial will is breathed out surely, hearing-improing and eyesight improving.Pharmacological action:. calm, syngignoscism: oral or lumbar injection all behaves with composure and somnolence Semen Ziziphi Spinosae decoct to rat, no matter daytime or night, Semen Ziziphi Spinosae all can show above-mentioned effect.Calm index when white mice is oral is 1.95, shows synergism with barbiturate, and after Semen Ziziphi Spinosae is used continuously 6 days, animal sleep can shoal, and namely decreased duration produces toleration, but drug withdrawal can disappear after 1 week.

Fructus Schisandrae Chinensis: Fructus Schisandrae Chinensis is used as medicine with rhizome.There is boosting qi and nourishing yin, spleen invigorating, lung moistening, kidney benefiting functions.Be used for the treatment of weakness of the spleen and stomach, fatigue and asthenia, xerostomia lack of appetite, deficiency of the lung cough caused by dryness, asthenia of essence and blood, interior-heat such as to be quenched one's thirst the disease.Very effective in cure for diabetes.Fructus Schisandrae Chinensis has blood pressure lowering, blood sugar lowering, blood fat reducing, atherosclerosis, and slow down aging and the effect such as antibacterial, Fructus Schisandrae Polysaccharide has Immunestimulatory effect.For lung-dryness syndrome due to deficiency of YIN, the few expectorant of dry cough, and the chronic cough chronic cough etc. of YIN-deficiency of the lung and kidney.For weakness of the spleen and stomach.Both tonifying spleen-yin, again strengthening the spleen gas, obtain dizzy, soreness of the waist and knees for deficiency of kidney essence, early whitening of beard and hair and quenching one's thirst.Fructus Schisandrae Chinensis strengthening bone and muscle, bone strengthening marrow, bleaches and sends out; The cough expectorant be suitable for caused by lack of lung-YIN is few, dry cough without phlegm, the diseases such as hemoptysis.

Poria sweet in the mouth, light, property are put down, and are used as medicine and have the function of promoting diuresis to eliminate damp pathogen, strengthening the spleen stomach function regulating, mind tranquilizing and the heart calming.Modern medicine study: Poria energy enhancing human body immunity function, pachyman has obvious antitumor and protects the liver dirty effect.Poria slightly sweet flavor is put down, and enters the heart, lung, spleen channel.There is eliminating dampness and diuresis, invigorating the spleen and regulating the stomach, effect of mind tranquilizing and the heart calming.Can control dysuria, edema distension, phlegm retention cough with dyspnea, vomiting, malignant histocytosis, has loose bowels, seminal emission, stranguria with turbid discharge, palpitation with fear, forgetful disease such as grade.The diuretic of Poria, is reached by strong activating the spleen pulmonary function, different from the Chinese medicine of other direct diuretic.For diarrhea due to hypofunction of the spleen, leukorrhagia Poria can spleen invigorating, again can eliminating dampness by diuresis, and for having loose bowels caused by insufficiency of the spleen dysfunction of the spleen in transportation and transformation, leukorrhagia, application Poria has the effect of giving consideration to both the incidental and fundamental, the compatibilities such as normal and Radix Codonopsis, the Rhizoma Atractylodis Macrocephalae, Rhizoma Dioscoreae.Can be used as tonifying the lung spleen, control the adjuvant of the deficiency of vital energy.

Chinese medicine Radix Polygoni Multiflori have Radix Polygoni Multiflori and Radix Polygoni Multiflori Preparata point, direct slicing is used as medicine for Radix Polygoni Multiflori, dries be used as medicine for Radix Polygoni Multiflori Preparata with Semen sojae atricolor liquor after mixing steaming.The function of the two is different: the removing toxic substances of Radix Polygoni Multiflori function, eliminating carbuncle, loosening bowel to relieve constipation, is usually used in treatment scrofula carbuncle sore, rubella pruritus, dryness of the intestine constipation; The invigorating the liver and kidney of Radix Polygoni Multiflori Preparata function, benefiting essence-blood, black beard and hair, bone and muscle strengthening, for blood deficiency and yellow complexion, vertigo and tinnitus, early whitening of beard and hair, soreness of the waist and knees, numb limbs and tense tendons, bleeding not during menses, chronic malaria body void etc.Radix Polygoni Multiflori Preparata of the present invention is the good merchantable brand of invigrating kidney.

The acid of Herba Ecliptae sweet in the mouth, cool in nature, enter Liver and kidney two warp, nontoxic.Cure mainly: nourishing the liver and kidney, cooling blood for hemostasis, various haematemesis can be controlled, the diseases such as enterorrhagia.Smash juice painting eyebrow to send out, can promote hair growth, for oral administration have black hair, hair color effect.Tang Materia Medica is said, Herba Ecliptae " main dysentery, acupuncture skin ulcer is sent out, big vast blood can not the person of stopping respect it ".Also say in " Japan hanako materia medica ", Herba Ecliptae can " logical small intestinal, applies all skin ulcers and silkworm Guo for evacuation of pus, hemostasis "." the southern regions of the Yunnan Province book on Chinese herbal medicine " carries, Herba Ecliptae " teeth consolidating, black hair wash nine kinds of hemorrhoid ".

Cortex Moutan is cold in nature, bitter in the mouth; Cool; Be slightly cold.GUIXIN; Liver; Kidney; Lung meridian.Clearing away heat and cooling blood; Promoting blood circulation to remove blood stasis.The epidemic febrile disease pathogenic heat attacking blood system in febrile diseases; Send out speckle; Tell nosebleed; The calentura later stage, hot Fu Yin divided heating; Deficiency of YIN osteopyrexia and fever; Amenorrhea due to stagnation of blood; Dysmenorrhea; Carbuncle sore tumefacting virus; Fall and flutter the pain of injury; Beriberoid pyretic arthralgia.To the mice male Cortex Moutan of gavage respectively, Cortex Moutan phenol, paeoniflorin, oxypaeoniflorin, benzoylpaeoniflorin, all can promote that intravenous carbon granules cleans up speed in blood, even if mononuclear phagocyte system function is in low state also have facilitation, microscopy is shown in that in liver, in Kupffer cell and spleen, macrophage phagocytic activity strengthens.Paeoniflorin, oxypaeoniflorin also can strengthen the phagocytic function of Turnover of Mouse Peritoneal Macrophages to emulsion in vitro.After Cortex Moutan liquid can make its spleen haemolysis to mouse peritoneal injection, speckle number increases.With Cortex Moutan phenol to the every large 25mg/kg of mouse peritoneal injection, be used in conjunction 6 days, can make that spleen is heavy obviously to be increased, and cortisone, the alleviating of caused by cyclophosphamide thymic weight can be resisted.Therefore male Cortex Moutan all has potentiation to body fluid and cellular immunization." Bencao Jingshu ": Cortex Moutan, its bitter in the mouth and micro-pungent, its gas is cold and nontoxic, pungently gathers with eliminating stagnation, and bitter cold is except heat in blood, and enter blood system, the key medicine of removing heat from blood heat also.Cold and heat person, the time of YinXuXueRe also.Apoplexy clonic convulsion, convulsion, infantile convulsion, all yin asthenia generating intrinsic heat, the event of nutrient QI and blood being insufficient.Heat goes then blood cool, and cool then fresh blood is raw, cloudy gas is multiple, and then fire is scorching and without the card because of hot air wind producing again for cloudy gas, therefore knows master's.

The specific embodiment of the invention:

Specific embodiment 1

Put by Herba Epimedii and soak in ethanol, extract, medicinal residues water extraction, as component 1;

The extraction of other crude drug, purchases, as component 2;

By component 1 and component also 2-in-1, add dextrin after concentrated or Mel is in harmonious proportion, bottle after ultraviolet sterilization.

Specific embodiment 2

Herba Epimedii 5000g is put into the ethanol accounting for its quality 10 times amount, heating and refluxing extraction 2 times, each 1-2 hour, remove supernatant, merge extractive liquid, 100-110 order filters, be the ultrafiltration post ultrafiltration of 6000-10000 again through the molecular weight that dams, when ultrafiltrate concentrating under reduced pressure relative density is 80 DEG C 1.36 extractum, heating be concentrated into paste, stand for standby use; Again the medicinal residues that above-mentioned ethanol extraction is crossed are added the water heating and refluxing extraction 2 times accounting for its quality 10 times amount, remove supernatant, merge extractive liquid, 100-110 order filters, be the ultrafiltration post ultrafiltration of 6000-10000 again through the molecular weight that dams, when ultrafiltrate concentrating under reduced pressure relative density is 80 DEG C 1.36 extractum, heating be concentrated into paste, stand for standby use; Extract extractum by above-mentioned two kinds to merge, reducing pressure after reclaiming ethanol, it is concentrated to put into concentrator, reduces pressure extractum becomes component 1.

By all the other crude drug Herba Ecliptae 1500g, Fructus Schisandrae Chinensis 1500g, Poria 1500g, Radix Polygoni Multiflori Preparata 1500g, Fructus Ligustri Lucidi 1500g, Radix Paeoniae Alba 1500g, Rhizoma Acori Graminei 1500g, Semen Platycladi 1500g, Fructus Mori 1500g, Concha Margaritifera 1500g, Radix Polygalae 1500g, Cortex Albiziae 1500g, Radix Ophiopogonis 1500g, Semen Ziziphi Spinosae 1500g, Caulis Polygoni Multiflori 1500g, Rhizoma Coptidis 1500g, Rhizoma Polygonati Odorati 1500g, Cortex Moutan 1500g, Radix Curcumae 1500g, add 10 times of water gagings, then put into extraction pot heated and boiled 1 hour, filter, filtrate is for subsequent use; Filtering residue adds water, and second time heating, boils 45 minutes, and filter, filtrate is for subsequent use; Filtering residue adds water again, heated and boiled half an hour third time, filters; Three filtrates be combined, by filtered through gauze, add 50-60% ethanol in residue, 60 DEG C-70 DEG C are continued lixiviate 2h, and every 10min stirs once, filtered through gauze, merge lixiviating solution, are condensed into pasty state, become component 2.

Extract extractum by above-mentioned two kinds to insert in economic benefits and social benefits vacuum concentrator, when being concentrated into 90 DEG C, relative density is the pasty state of 1.50, sets to 0 ~ 5 DEG C of deepfreezes 24 hours; Cold preservation liquid is added the filter aid kieselguhr of 0.3%, filter, filtrate is inserted in economic benefits and social benefits vacuum concentrator again, is concentrated into every 1ml containing 0.1g crude drug amount, then adds Mel or dextrin mediation bottling.

Specific embodiment 3

Herba Epimedii 4000g is soaked 2-5 hour in water, add respectively account for its quality 8 times amount, 6 times amount water extraction 2 times, extraction time is 2 hours, 1 hour; Merge 2 extracting solution, extracting solution is centrifugal by tubular type, obtains centrifugal liquid, and centrifugal liquid is crossed D 101 macroporous resin and adsorbed, and first washes remove impurity with water, then carries out eluting with the ethanol of 70%, collects eluent, concentrating under reduced pressure, one-tenth extractum, for subsequent use; The medicinal residues of Herba Epimedii said extracted crossed add ethanol, add respectively account for its quality 10 times amount, 8 times amount ethanol warm macerating 2 times, the warm macerating time is 2 hours, 1.5 hours, and temperature is 85 DEG C, merges 2 warm macerating liquid; Warm macerating liquid is evaporated to 1.25 extractum when density is 60 DEG C, and extractum is added the extraction into ethyl acetate secondary of its 4 times amount, extract has reclaimed ethyl acetate, becomes extractum, for subsequent use.Above-mentioned two kinds of extraction components are merged, when concentrating under reduced pressure relative density is 80 DEG C 1.35 filtrate, reclaim ethanol, above-mentioned two kinds of extracting solution are inserted in economic benefits and social benefits vacuum concentrator, when being concentrated into 90 DEG C, relative density is the concentrated solution of 1.60, sets to 0 ~ 5 DEG C of deepfreezes 24 hours; Cold preservation liquid is added the filter aid kieselguhr of 0.3%, filter, filtrate is inserted in economic benefits and social benefits vacuum concentrator again, is concentrated into every 1ml containing 0.1g crude drug amount, becomes component 1.By all the other crude drug Herba Ecliptae 1200g, Fructus Schisandrae Chinensis 1100g, Poria 1100g, Radix Polygoni Multiflori Preparata 1100g, Fructus Ligustri Lucidi 1200g, Radix Paeoniae Alba 1100g, Rhizoma Acori Graminei 1200g, Semen Platycladi 1100g, Fructus Mori 1100g, Concha Margaritifera 1200g, Radix Polygalae 1200g, Cortex Albiziae 1100g, Radix Ophiopogonis 1100g, Semen Ziziphi Spinosae 1100g, Caulis Polygoni Multiflori 1100g, Rhizoma Coptidis 1100g, Rhizoma Polygonati Odorati 1100g, Cortex Moutan 1100g, Radix Curcumae 1100g, add 10 times of water gagings, then put into extraction pot heated and boiled 1 hour, filter, filtrate is for subsequent use; Filtering residue adds water, and second time heating, boils 45 minutes, and filter, filtrate is for subsequent use; Filtering residue adds water again, heated and boiled half an hour third time, filters; Three filtrates be combined, by filtered through gauze, add 50-60% ethanol in residue, 60 DEG C-70 DEG C are continued lixiviate 2h, and every 10min stirs once, filtered through gauze, merge lixiviating solution, are condensed into pasty state, become component 2.By component 1 and component also 2-in-1, then put into concentrating under reduced pressure tank, reclaim ethanol, when being concentrated into 90 DEG C, relative density is the extractum of 1.50, is concentrated into every 1ml containing 0.1g crude drug amount, then adds Mel or dextrin and is in harmonious proportion bottling.

Specific embodiment 4

Get Herba Epimedii 4500g, Herba Ecliptae 1200g, Fructus Schisandrae Chinensis 1300g, Poria 1300g, Radix Polygoni Multiflori Preparata 1200g, Fructus Ligustri Lucidi 1200g, Radix Paeoniae Alba 1300g, Rhizoma Acori Graminei 1200g, Semen Platycladi 1200g, Fructus Mori 1200g, Concha Margaritifera 1200g, Radix Polygalae 1200g, Cortex Albiziae 1300g, Radix Ophiopogonis 1200g, Semen Ziziphi Spinosae 1200g, Caulis Polygoni Multiflori 1200g, Rhizoma Coptidis 1200g, Rhizoma Polygonati Odorati 1200g, Cortex Moutan 1200g, Radix Curcumae 1200g, all crude drug are put into 10 times amount ethanol to soak more than 24 hours, 2 extracting solution each 1 ~ 2 hour, are merged and leave standstill by heating and refluxing extraction 2 times; The medicinal residues of the component crude drug medical material crossed by above-mentioned ethanol extraction again add 10 times of water gaging heating and refluxing extraction 2 times, each 1 ~ 2 hour, are merged by 2 extracting solution and leave standstill; Above-mentioned two kinds of extracting solution are merged, after reclaiming ethanol decompression put into concentrator concentrated time, when being concentrated into 90 DEG C, relative density is the concentrated solution of 1.50, sets to 0 ~ 5 DEG C of deepfreezes 24 hours; Cold preservation liquid is added the filter aid kieselguhr of 0.3%, filter, filtrate is inserted in economic benefits and social benefits vacuum concentrator again, is concentrated into every 1ml containing 0.1g crude drug amount; Then add dextrin to be in harmonious proportion, pack after ultraviolet radiation for sterilizing and disinfecting.

Pharmacological toxicity is tested

One, skin irritation test

1, experimental technique

Depilatory is evenly coated by guinea pig back spinal column both sides, makes unhairing scope about 20 square centimeters.Clean depilatory returns cage to observe 24 hours, often organize Cavia porcellus and be coated with the present invention's (pill) 4,8 and 12g respectively, respectively containing crude drug 92mg, 184mg and 368mg, another group is coated with the every twice-daily of solvent 0.8ml, continuous one week, after experiment terminates, Cavia porcellus is put to death, the heart, liver, kidney and depilation skin do pathological examination.

2, result

Above-mentioned three groups of medication Cavia porcellus trunks depilation district, having no local skin has edema, hyperemia, erythema, petechia and ulcer.Medication group Cavia porcellus chroma of hair, ingest, the matched group no significant difference such as extremity are movable, histopathologic examination, the administration group heart, liver, kidney and depilation skin with contrast word more also no significant difference.

Result is pointed out, and the present invention, without local irritant effect, also has no general toxicity performance.Show invention formulation use safety.

Two, animal acute toxicity test research:

The present invention, through trial test, can not measure LD 50, therefore measures maximum dosage-feeding.Adopt rectal delivery, the maximum dosage-feeding on the one recording mice is 62.7g/kg (in medicated powder), be about 1567.5 times (people is in 60kg) intending clinical people's consumption, the maximum dosage-feeding 62.7g/kg (in medicated powder) on the one of rat, is about 1567.5 times that intend clinical people's consumption.The equal Continuous Observation of laboratory animal one week, its outward appearance sign, the mental status, diet, drinking-water, feces etc. are showed no obvious abnormalities.At the end of experiment, all healthy survival of animal, body weight increases.

Three, long-term toxicity test for animals research:

One, test material and method

(1) test medicine: the oral liquid that the embodiment of the present invention is made.

(2) laboratory animal, healthy rat Wistar, body weight 100-130g, 140 male and female half and half, by sex and body weight random packet, four groups, often organize 35.1, take test medicine for 2,3 groups, dosage is respectively 9.6/kg/d, 6.4/kg/d, 3.2/kg/d.4 groups contrast to the distilled water of respective volume amount, the equal gavage enteral administration of rat, often organize and be once used in conjunction 6 months, put to death half, and all death in 12 days is observed in remaining 1/2 drug withdrawal.

Two, test index

(1) general status comprises body weight, diet, emotion, movable energy.

(2) hemogram checking: red blood cell count(RBC), numeration of leukocyte, platelet count, hemogram etc., quantitative hemoglobin counts.

(3) biochemical analysis, serum albumin quantitatively, cholesterol, blood sugar quantitative.

(4) pathologic diagnosis: get half animal after experimental result and weigh, sacrificed by decapitation, gets blood, solution is treated others for earnest simultaneously, liver, kidney, lung, brain, spleen, gastrointestinal, bladder, adrenal gland do pathologic diagnosis.

Three, result of the test: show through rat chronic toxicity test, with the heavy dose of coloclysis of the present invention six months, increases the growth of rat, body weight, all has no side effect to brain, Liver and kidney function.Do not find that infringement changes to each histoorgan of rat, blood circulation, medication group compares no significant difference with matched group.Illustrate that medicine of the present invention has warming and recuperating the kidney-YANG, QI invigorating, enriches blood, strengthens the drug effects such as metabolism, avirulence.

Clinical trial:

The present invention is the health care oral liquid be made up of medicines such as Radix Polygalae, Radix Polygoni Multiflori Preparata, Herba Epimedii, Radix Paeoniae Rubra, has invigorating brain and relieving mental uneasiness, and spermatogenesis mends marrow, effect of liver heat removing and eyesight improving benefiting QI and nourishing blood, for neurasthenia, insomnia, the disease such as forgetful, dizzy.Observe the present invention herein to monoamine neurotransmitter 5 one HT in underage mouse ability of learning and memory and brain, DA, NA, the impact of 5 one HIAA content.

1 test material

1.1 medicine the present invention keep healthy health care oral liquid, are prepared by the method for embodiment 2 by Pharmacy department of the court; Positive control drug, ANSHEN BUNAO YE, the accurate word Z22022453 of traditional Chinese medicines, manufacturing enterprise: Jilin Aodong Yanbian Medicine Industry Co., Ltd.

1.2 animal Kunming mouse 11 ~ 15g, male and female half and half, purchased from Changchun Biological Products Institute, credit number: SCXK.2002.0001.

1.3 instrument Y. type water mazes; ES.180 electronic analytical balance, Shenyang Long Teng company produces; RF.540 type spectrofluorophotometer, Japanese Shimadzu Corporation produces.

1.4 reagent 5. hydroxytryptamine creatinine sulfates, China Drug Co.'s Solution on Chemical Reagents in Shanghai purchasing and supply station, lot number: 65-02.28, Switzerland's import subpackage, purity 99%; 5. hydroxyindole acetic acid, Sigma product, lot number: 60k1083, purity 98% ~ 100%; Potassium bitartrate norepinephrine, Shanghai Hefeng Pharmaceutical Co., Ltd. produces, lot number: 0205037,2mg/mL; Dopamine hydrochloride, is produced by Shanghai Hefeng Pharmaceutical Co., Ltd., lot number: 0204011,20mg/2mL; Other reagent is domestic analytical pure.

2 methods and result

Mice 80 is got on the impact (water maze) of underage mouse ability of learning and memory, is divided at random: blank group, ANSHEN BUNAO YE liquid 13mL/kg administration group, of the present invention group of 10.0,5.0,2.5mL/kg 3 dosed administration groups.Administration every day 1 time, in the 11d of first administration, successive administration 15d, starts that mice is placed in water maze and trains.Experiment adopts Y. type water labyrinth apparatus.This device divides long arm end and two branch galianconism ends.Left side galianconism end is equipped with platform, stays in part, have lighting above platform as mice.Three branch arm ends leave the opening of a 10cm, and mid portion black acrylic covers, as secret tunnel.Depth of water 10cm, water temperature (30 ± 2) DEG C, train 5d altogether, every animal trains 10 times every day, during training, the afterbody of mice is put into water lightly towards two forks in labyrinth, directly reaching in 15S with it has the platform of light source for correct response, otherwise is considered as mistake, record the number of times that every animal is made a mistake every day, and calculate 5d average achievement.Experimental data represents with-X ± S, compares by the sample 2.1 mean t method of inspection, the results are shown in Table 1 between group.

Table 1 the present invention is on the impact (x ± s, n=16) of underage mouse ability of learning and memory

Note: with blank group than * P<0.05, * * P<0.01, * * * P<0.001, following table all with.

From table 1 result, 10.0mL/kg dosage group of the present invention, starts the errors number that just can reduce mice significantly in the 3d of training, and along with the prolongation of administration time, errors number reduces gradually, illustrates that it can strengthen ability of learning and memory in mice.

2.2 impacts on monoamine transmitter contents in underage mouse brain

Get mice 80, the same grouping administration, successive administration 15d, after last administration, 1h is by sacrifice of animal, gets cerebral tissue rapidly, puts into liquid nitrogen immediately and fills with freezing, take out homogenate in batches, by 5-HT, DA, NA in fluorescence spectrophotometry Mice brain tissues, the content of 5-HIAA, experimental data represents with x ± s, compares by the sample average t method of inspection, the results are shown in Table 2 between group.

Table 2 the present invention is on the impact (x ± s, n=16) of monoamine transmitter contents in underage mouse brain

From table 2 result, the present invention can increase 5-HT, DA, NA content in mouse brain tissue significantly.

2.3 get mice 80 to the impact of underage mouse thymus, spleen, Weight-index, with 2.1 grouping administrations, after last administration, 1h is by sacrifice of animal, get thymus, spleen, adrenal gland weigh calculating organ index, experimental data represents with x ± s, compare between group by the sample average t method of inspection, the results are shown in Table 3.

Table 3 the present invention is correlated with on underage mouse the impact (x ± s, n=16) of organ index

From table 3 result, the present invention can increase Weight-index significantly.

3 discuss

Learning and memory is one of Premium Features of brain, is the important indicator weighing animal intelligent development, and learning and memory is realized by a series of physiological and biochemical procedure of nervous system.Monoamine neurotransmitter is to attention, and memory and respond have important regulating action, and it comprises NA, DA, 5-HT, 5-HIAA etc., NA, DA, 5-HT rich content in synapse, and it not only transmits relevant with synapse, and relevant with synapse maturation.The minimizing of NA, DA, 5-HT, 5-HIAA, must affect the balance of neurotransmitter in brain, cause intellectual damage and go down.Experimental result shows, ANSHEN BUNAO YE can strengthen the ability of learning and memory of underage mouse, also show that it can improve NA in animal brain simultaneously, DA, the content of 5-HT, ANSHEN BUNAO YE has been applied for many years on I narrow-necked earthen jar bed, effect obviously, determined curative effect, but be used for the treatment of forgetful mechanism of action to it and never carry out deep research, point out herein from the result that affects of monoamine neurotransmitter aspect in immature animal brain, the present invention can by activating brain metabolism, increase multiple neurohumoral quantity in cerebral cortex, thus reach the function of hypermnesis.Experimental result also shows the present invention can increase underage mouse adrenal gland weight, and administration group and matched group have notable difference, and this also may be relevant containing the kidney-reinforcing drug such as Radix Polygoni Multiflori Preparata, Herba Epimedii with its side.

Herba Epimedii, property is worked hard, and taste temperature, returns Liver and kidney warp.Herb hyoscine.Cure mainly impotence and premature ejaculation, pain in the low back and legs, numb limbs and tense tendons, hemiplegia, neurasthenia, forgetful, tinnitus, dizzy disease such as grade.Herba Epimedii is Chinese conventional Chinese medicine.Primary treatment kidney invigorating and YANG supporting, expelling wind and removing dampness.Control sexual impotence erectile problem, hemiplegia is unsuccessful, dribbling urination, and muscles and bones contraction is anxious, and cough, optic atrohic blindness after being ill, toothache caused by fire of deficiency type, hemiplegia, weakness of the waist and knees, rheumatic arthralgia, extremity are meciless." herbal classic " carries it: main impotence is hindered absolutely, pains in the penis.Diuresis, physical strength profiting, Qiang Zhi." not Lu " carries it: hard muscles and bones.Disappear scrofula, red carbuncle; Skin ulcer is arranged at bottom, washes, and goes out worm." Japan hanako materia medica " carries it: control all cold wind consumptive disease, and mend waist knee joint, heart tonifying power, husband is exhausted, and sun does not rise, the exhausted cloudy loss of fecundity of woman, and muscles and bones contraction is anxious, and extremity are not appointed, and old man's dusk octogenarian, the middle age is forgetful." Elementary Medicine " carries it: the kidney invigorating is empty, supporing yang.Control hemiplegia hands and feet caused by paralysis, limbs skin is meciless.Spermatogenesis of the present invention mends marrow, and benefiting QI and nourishing blood, strong brain is calmed the nerves.For the dizziness caused by deficiency of kidney-essence, deficiency of both QI and blood, weak, forgetful, insomnia; Neurasthenia is complete.

The present invention is the health product of green health environmental protection.Add some invigorating brain and relieving mental uneasiness, blood circulation promoting and blood stasis dispelling, the natural Chinese medicine of inrigorating qi and promoting blood circulation, dispersing the stagnated live-QI to relieve the stagnation of QI, suppressing the hyperactive liver and subsiding YANG, enriching yin and nourishing kidney, heat-clearing and toxic substances removing, clearing away heat-fire, spermatogenesis mends marrow, benefiting QI and nourishing blood, strong brain is calmed the nerves, regulate the metabolism function of human body, compatibility science of the present invention, pure Chinese medicinal preparation, without any toxic and side effects, and instant effect, mouthfeel is good, can life-time service.

Claims

1. the Herba Epimedii oral liquid of an Anshen Bunao, it is characterized in that, described oral liquid comprises the crude drug of following parts by weight: Herba Epimedii 10 ~ 50 parts, Herba Ecliptae 10 ~ 20 parts, Fructus Schisandrae Chinensis 10 ~ 20 parts, 10 ~ 20 parts, Poria, Radix Polygoni Multiflori Preparata 10 ~ 20 parts, Fructus Ligustri Lucidi 10 ~ 20 parts, the Radix Paeoniae Alba 10 ~ 20 parts, Rhizoma Acori Graminei 10 ~ 20 parts, Semen Platycladi 10 ~ 20 parts, Fructus Mori 10 ~ 20 parts, Concha Margaritifera 10 ~ 20 parts, Radix Polygalae 10 ~ 20 parts, Cortex Albiziae 10 ~ 20 parts, Radix Ophiopogonis 10 ~ 20 parts, Semen Ziziphi Spinosae 10 ~ 20 parts, Caulis Polygoni Multiflori 10 ~ 20 parts, Rhizoma Coptidis 10 ~ 20 parts, Rhizoma Polygonati Odorati 10 ~ 20 parts, Cortex Moutan 10 ~ 20 parts, Radix Curcumae 10 ~ 20 parts.

2. the Herba Epimedii oral liquid of Anshen Bunao according to claim 1, it is characterized in that, described oral liquid comprises the crude drug of following parts by weight: Herba Epimedii 10 ~ 30 parts, Herba Ecliptae 10 ~ 15 parts, Fructus Schisandrae Chinensis 10 ~ 15 parts, 10 ~ 15 parts, Poria, Radix Polygoni Multiflori Preparata 10 ~ 15 parts, Fructus Ligustri Lucidi 10 ~ 15 parts, the Radix Paeoniae Alba 10 ~ 15 parts, Rhizoma Acori Graminei 10 ~ 15 parts, Semen Platycladi 10 ~ 15 parts, Fructus Mori 10 ~ 15 parts, Concha Margaritifera 10 ~ 15 parts, Radix Polygalae 10 ~ 20 parts, Cortex Albiziae 10 ~ 20 parts, Radix Ophiopogonis 10 ~ 20 parts, Semen Ziziphi Spinosae 10 ~ 20 parts, Caulis Polygoni Multiflori 10 ~ 20 parts, Rhizoma Coptidis 10 ~ 20 parts, Rhizoma Polygonati Odorati 10 ~ 20 parts, Cortex Moutan 10 ~ 20 parts, Radix Curcumae 10 ~ 20 parts.

3. the Herba Epimedii oral liquid of a kind of Anshen Bunao according to claim 1, it is characterized in that, described oral liquid comprises the crude drug of following parts by weight: Herba Epimedii 10 ~ 40 parts, Herba Ecliptae 10 ~ 20 parts, Fructus Schisandrae Chinensis 10 ~ 20 parts, 10 ~ 20 parts, Poria, Radix Polygoni Multiflori Preparata 10 ~ 20 parts, Fructus Ligustri Lucidi 10 ~ 20 parts, the Radix Paeoniae Alba 10 ~ 20 parts, Rhizoma Acori Graminei 10 ~ 20 parts, Semen Platycladi 10 ~ 20 parts, Fructus Mori 10 ~ 20 parts, Concha Margaritifera 10 ~ 20 parts, Radix Polygalae 10 ~ 15 parts, Cortex Albiziae 10 ~ 15 parts, Radix Ophiopogonis 10 ~ 15 parts, Semen Ziziphi Spinosae 10 ~ 15 parts, Caulis Polygoni Multiflori 10 ~ 15 parts, Rhizoma Coptidis 10 ~ 15 parts, Rhizoma Polygonati Odorati 10 ~ 15 parts, Cortex Moutan 10 ~ 15 parts, Radix Curcumae 10 ~ 15 parts.

4. the preparation method of the Herba Epimedii oral liquid of Anshen Bunao according to any one of claims 1 to 3, it is characterized in that, the preparation process of described oral liquid comprises:

The preparation of a, Herba Epimedii; For component 1

B, all the other crude drug heat are extracted 2 times, are component 2

5. the preparation method of the Herba Epimedii oral liquid of Anshen Bunao according to claim 4, is characterized in that, in described step a, Herba Epimedii is put into the ethanol accounting for its quality 10 times amount, heating and refluxing extraction 2 times, each 1-2 hour, removes supernatant, merge extractive liquid, 100-110 order filters, then is the ultrafiltration post ultrafiltration of 6000-10000 through the molecular weight that dams, when ultrafiltrate concentrating under reduced pressure relative density is 80 DEG C 1.36 extractum, heating is concentrated into paste, stand for standby use; Again the medicinal residues that above-mentioned ethanol extraction is crossed are added the water heating and refluxing extraction 2 times accounting for its quality 10 times amount, remove supernatant, merge extractive liquid, 100-110 order filters, be the ultrafiltration post ultrafiltration of 6000-10000 again through the molecular weight that dams, when ultrafiltrate concentrating under reduced pressure relative density is 80 DEG C 1.36 extractum, heating be concentrated into paste, stand for standby use; Extract extractum by above-mentioned two kinds to merge, after reclaiming ethanol, the concentrated extractum that reduces pressure to obtain of concentrator is put in decompression.

6. the preparation method of the Herba Epimedii oral liquid of Anshen Bunao according to claim 4, it is characterized in that, in described step b, getting all the other crude drug steeps in water, soak more than 2 hours, add the water extraction 2 times of 8 times amount of crude drug medicine gross weight, 6 times amount respectively, extraction time is 2 hours, 1 hour; Merge 2 extracting solution, extracting solution is centrifugal by tubular type, obtains centrifugal liquid, and centrifugal liquid is crossed D101 macroporous resin and adsorbed, and first washes remove impurity with water, then carries out eluting with the ethanol of 70%, collects eluent, concentrating under reduced pressure, one-tenth extractum.

7. the preparation method of the Herba Epimedii oral liquid of Anshen Bunao according to claim 4, it is characterized in that, in described step c, extract extractum by above-mentioned two kinds and insert in economic benefits and social benefits vacuum concentrator, when being concentrated into 90 DEG C, relative density is the pasty state of 1.50, sets to 0 ~ 5 DEG C of deepfreezes 24 hours; Cold preservation liquid is added the filter aid kieselguhr of 0.3%, filter, filtrate is inserted in economic benefits and social benefits vacuum concentrator again, is concentrated into every 1ml containing 0.1g crude drug amount, then adds Mel or dextrin mediation bottling.

8. the preparation method of the Herba Epimedii oral liquid of Anshen Bunao according to any one of claims 1 to 3, it is characterized in that, the preparation method of described oral liquid comprises:

9. the preparation method of the Herba Epimedii oral liquid of Anshen Bunao according to claim 8, it is characterized in that, in described step a, all crude drug are steeped 1-5 hour in the ethanol accounting for its quality 10 times amount, heating extraction 2 times, each 1-2 hour, removes supernatant, merge extractive liquid, 100-110 order filters, be the ultrafiltration post ultrafiltration of 6000-10000 again through the molecular weight that dams, when ultrafiltrate concentrating under reduced pressure relative density is 80 DEG C, the extractum of 1.36 is stand-by, is component 1

In described step b, the medicinal residues extracted are added 10 times of water gagings, then put into extraction pot heated and boiled 1 hour, filter, filtrate is for subsequent use; Filtering residue adds water, and second time heating, boils 45 minutes, and filter, filtrate is for subsequent use; Filtering residue adds water again, heated and boiled half an hour third time, filters; Three filtrates be combined, by filtered through gauze, add 50-60% ethanol in residue, 60 DEG C-70 DEG C are continued lixiviate 2h, and every 10min stirs once, filtered through gauze, merge lixiviating solution, are condensed into pasty state, become component 2.

10. the preparation method of the Herba Epimedii oral liquid of Anshen Bunao according to claim 8, it is characterized in that, in described step c, two kinds of components are merged, reclaim decompression after ethanol put into concentrator concentrated time, when being concentrated into 90 DEG C, relative density is the concentrated solution of 1.50, sets to 0 ~ 5 DEG C of deepfreezes 24 hours; Cold preservation liquid is added the filter aid kieselguhr of 0.3%, filter, filtrate is inserted in economic benefits and social benefits vacuum concentrator again, is concentrated into every 1ml containing 0.1g crude drug amount, concentrated after stirring, and puts into Mel and is in harmonious proportion, ultraviolet virus killing bottling.