CN110249047A - 使用源自干细胞的施旺细胞的药物发现方法 - Google Patents

使用源自干细胞的施旺细胞的药物发现方法 Download PDF

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CN110249047A
CN110249047A CN201780083272.4A CN201780083272A CN110249047A CN 110249047 A CN110249047 A CN 110249047A CN 201780083272 A CN201780083272 A CN 201780083272A CN 110249047 A CN110249047 A CN 110249047A
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cells
days
cell
schwann
differentiation
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L·施图德
F·法塔希
Z·加扎扎德
S·陈
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Cornell University
Memorial Sloan Kettering Cancer Center
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Cornell University
Memorial Sloan Kettering Cancer Center
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CN201780083272.4A 2016-11-14 2017-11-14 使用源自干细胞的施旺细胞的药物发现方法 Pending CN110249047A (zh)

Applications Claiming Priority (3)

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US201662421816P 2016-11-14 2016-11-14
US62/421,816 2016-11-14
PCT/US2017/061549 WO2018090006A1 (en) 2016-11-14 2017-11-14 Methods for drug discovery using stem cell-derived schwann cells

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EP (2) EP3538115A4 (enExample)
JP (4) JP2019535263A (enExample)
KR (1) KR20190077095A (enExample)
CN (2) CN110691601A (enExample)
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CA (2) CA3043226A1 (enExample)
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Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL272246B1 (en) 2017-07-28 2025-09-01 Applied Therapeutics Inc Derivatives of 2-(4-oxo/thioketone/azo-3-((substituted)benzo[d]thiazol-2-yl)methyl)- 3,4-dihydrothieno[3,4-d]pyridazin-1-yl)acetic acid for use as aldose reductase inhibitors in treating galactosemia or preventing complications associated with galactosemia
WO2019099902A1 (en) * 2017-11-17 2019-05-23 The Research Foundation for State University of New York A method for treating damaged peripheral nerves using x-ray microbeam irradiation
AU2018379993A1 (en) * 2017-12-04 2020-07-02 Axosim, Inc. Cell systems using spheroids and methods of making and using the same
US11845989B2 (en) 2019-01-23 2023-12-19 Regeneron Pharmaceuticals, Inc. Treatment of ophthalmic conditions with angiopoietin-like 7 (ANGPTL7) inhibitors
IL284700B2 (en) 2019-01-23 2025-10-01 Regeneron Pharma Treating eye conditions with angiopoietin-like 7 inhibitors
EP3966320A4 (en) * 2019-05-07 2023-10-25 University of Miami TREATMENT AND DETECTION OF HEREDITARY NEUROPATHIES AND ASSOCIATED DISORDERS
CN110157660A (zh) * 2019-06-10 2019-08-23 西安飞如生物科技有限公司 诱导成纤维细胞分泌抗菌肽的诱导培养基和方法、成纤维细胞及应用
JPWO2021166984A1 (enExample) * 2020-02-20 2021-08-26
EP3929281A1 (en) 2020-06-24 2021-12-29 Fachhochschule Technikum Wien Cell construct comprising schwann cells or schwann cell-like cells and a biocompatible matrix
CA3210480A1 (en) 2021-02-26 2022-09-01 Regeneron Pharmaceuticals, Inc. Treatment of inflammation with glucocorticoids and angiopoietin-like 7 (angptl7) inhibitors
AU2022291933A1 (en) * 2021-06-18 2024-01-18 Memorial Hospital For Cancer And Allied Diseases Methods of generating sacral neural crest lineages and uses thereof
CA3265079A1 (en) * 2022-08-16 2024-02-22 The Regents Of The University Of California CELLULAR COMPOSITIONS AND THEIR METHODS OF USE
WO2025170529A1 (en) 2024-02-09 2025-08-14 Hedlund Eva A method for deriving a multipotent neuromesodermal progenitor cell and/or daughter lineages, and related compositions

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102191217A (zh) * 2010-03-12 2011-09-21 上海市第一人民医院 一种诱导脐带间充质干细胞分化为类神经细胞的方法
US20130183674A1 (en) * 2010-05-25 2013-07-18 Memorial Sloan-Kettering Cancer Center Method of nociceptor differentiation of human embryonic stem cells and uses thereof
US20140038949A1 (en) * 2011-02-18 2014-02-06 Irm Llc Directed differentiation of oligodendrocyte precursor cells to a myelinating cell fate
CN105658787A (zh) * 2013-10-14 2016-06-08 加的夫大学学院咨询有限公司 神经元干细胞分化

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000023568A2 (en) * 1998-10-06 2000-04-27 Albert Einstein College Of Medicine Of Yeshiva University Methods and compositions for decreasing mitochondrial overproduction of reactive oxygen species in cells
CN101066472B (zh) * 2007-04-18 2010-05-19 中国人民解放军第二军医大学 组织工程化周围神经移植体及其制备方法
US8709805B2 (en) * 2009-08-07 2014-04-29 Kyoto University Canine iPS cells and method of producing same
WO2011046189A1 (ja) * 2009-10-15 2011-04-21 国立大学法人京都大学 神経変性疾患のモデル細胞、その製造方法及びその用途
CN103429742A (zh) * 2010-10-15 2013-12-04 康奈尔大学 用于治疗内分泌、胃肠或自体免疫性疾病的组合物和方法
EP2614829A1 (en) * 2012-01-11 2013-07-17 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Mammalian neural plate border stem cells capable of forming neural tube and neural crest cell lineages including central and peripheral neurons
CN105392881B (zh) * 2013-07-23 2020-09-01 豪夫迈·罗氏有限公司 体细胞基于小分子转化为神经嵴细胞
US10323763B2 (en) * 2013-07-30 2019-06-18 Water Technologies Corporation Check valve having polymeric seat and poppet
JP6723918B2 (ja) 2013-11-21 2020-07-15 メモリアル スローン ケタリング キャンサー センター ヒト多能性幹細胞からの機能的頭蓋プラコード派生体の特定
CN107075504B (zh) * 2014-09-12 2022-02-15 京都府公立大学法人 雪旺氏细胞及其制备方法
US20180140637A1 (en) * 2015-05-19 2018-05-24 Maya Sieber-Blum Epidermal neural crest stem cells as a source of schwann cells
KR101903458B1 (ko) * 2017-01-20 2018-10-02 한국생명공학연구원 슈반 세포 전구체 (Schwann cell precursor) 및 이로부터 분화된 슈반 세포 (Schwann cell)의 제조 방법

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102191217A (zh) * 2010-03-12 2011-09-21 上海市第一人民医院 一种诱导脐带间充质干细胞分化为类神经细胞的方法
US20130183674A1 (en) * 2010-05-25 2013-07-18 Memorial Sloan-Kettering Cancer Center Method of nociceptor differentiation of human embryonic stem cells and uses thereof
US20140038949A1 (en) * 2011-02-18 2014-02-06 Irm Llc Directed differentiation of oligodendrocyte precursor cells to a myelinating cell fate
CN105658787A (zh) * 2013-10-14 2016-06-08 加的夫大学学院咨询有限公司 神经元干细胞分化

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
GABSANG LEE ET AL.: "Isolation and directed differentiation of neural crest stem cells derived from human embryonic stem cells", 《NATURE BIOTECHNOLOGY》 *
LIAN-QING SUN ET AL.: "Protective effects of Salvianolic acid B on Schwann cells apoptosis induced by high glucose", 《NEUROCHEM RES》 *

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EP3538110A4 (en) 2020-04-22
US20190264173A1 (en) 2019-08-29
EP3538110A1 (en) 2019-09-18
US20190331666A1 (en) 2019-10-31
WO2018090006A1 (en) 2018-05-17
AU2017357072A1 (en) 2019-05-30
IL266554A (en) 2019-07-31
KR20190077095A (ko) 2019-07-02
JP2019535263A (ja) 2019-12-12
CA3043226A1 (en) 2018-05-17
EP3538115A1 (en) 2019-09-18
CN110691601A (zh) 2020-01-14

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Application publication date: 20190917