CN110226455B - Method for cultivating straw mushroom by using pure eucalyptus bark as main material - Google Patents
Method for cultivating straw mushroom by using pure eucalyptus bark as main material Download PDFInfo
- Publication number
- CN110226455B CN110226455B CN201910485417.4A CN201910485417A CN110226455B CN 110226455 B CN110226455 B CN 110226455B CN 201910485417 A CN201910485417 A CN 201910485417A CN 110226455 B CN110226455 B CN 110226455B
- Authority
- CN
- China
- Prior art keywords
- straw mushroom
- straw
- corn flour
- eucalyptus
- eucalyptus bark
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 239000000463 material Substances 0.000 title claims abstract description 93
- 240000006794 Volvariella volvacea Species 0.000 title claims abstract description 73
- 238000000034 method Methods 0.000 title claims abstract description 27
- 244000166124 Eucalyptus globulus Species 0.000 title claims abstract 11
- 240000008042 Zea mays Species 0.000 claims abstract description 44
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims abstract description 44
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims abstract description 44
- 235000005822 corn Nutrition 0.000 claims abstract description 44
- 238000000855 fermentation Methods 0.000 claims abstract description 31
- 230000004151 fermentation Effects 0.000 claims abstract description 31
- 235000013312 flour Nutrition 0.000 claims abstract description 31
- 240000007594 Oryza sativa Species 0.000 claims abstract description 25
- 235000007164 Oryza sativa Nutrition 0.000 claims abstract description 25
- 235000009566 rice Nutrition 0.000 claims abstract description 25
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 48
- 238000011081 inoculation Methods 0.000 claims description 24
- 239000007788 liquid Substances 0.000 claims description 24
- 241000881860 Paenibacillus mucilaginosus Species 0.000 claims description 20
- 239000007633 bacillus mucilaginosus Substances 0.000 claims description 20
- 244000063299 Bacillus subtilis Species 0.000 claims description 19
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 19
- 230000004913 activation Effects 0.000 claims description 17
- 239000000843 powder Substances 0.000 claims description 15
- 235000008733 Citrus aurantifolia Nutrition 0.000 claims description 14
- 235000011941 Tilia x europaea Nutrition 0.000 claims description 14
- 239000004571 lime Substances 0.000 claims description 14
- 230000000813 microbial effect Effects 0.000 claims description 14
- 239000003795 chemical substances by application Substances 0.000 claims description 13
- 238000003306 harvesting Methods 0.000 claims description 13
- 239000002609 medium Substances 0.000 claims description 13
- 150000004676 glycans Chemical class 0.000 claims description 10
- 229920001282 polysaccharide Polymers 0.000 claims description 10
- 239000005017 polysaccharide Substances 0.000 claims description 10
- 235000001674 Agaricus brunnescens Nutrition 0.000 claims description 9
- 230000007480 spreading Effects 0.000 claims description 7
- 238000003892 spreading Methods 0.000 claims description 7
- WFJIVOKAWHGMBH-UHFFFAOYSA-N 4-hexylbenzene-1,3-diol Chemical compound CCCCCCC1=CC=C(O)C=C1O WFJIVOKAWHGMBH-UHFFFAOYSA-N 0.000 claims description 6
- 239000001888 Peptone Substances 0.000 claims description 6
- 108010080698 Peptones Proteins 0.000 claims description 6
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 6
- 241000519995 Stachys sylvatica Species 0.000 claims description 6
- 235000015278 beef Nutrition 0.000 claims description 6
- 235000013339 cereals Nutrition 0.000 claims description 6
- 238000001816 cooling Methods 0.000 claims description 6
- 238000012258 culturing Methods 0.000 claims description 6
- 230000003203 everyday effect Effects 0.000 claims description 6
- 239000000284 extract Substances 0.000 claims description 6
- 238000010438 heat treatment Methods 0.000 claims description 6
- 238000002156 mixing Methods 0.000 claims description 6
- 235000019319 peptone Nutrition 0.000 claims description 6
- 238000007789 sealing Methods 0.000 claims description 6
- 238000005507 spraying Methods 0.000 claims description 6
- 239000008223 sterile water Substances 0.000 claims description 6
- 238000003756 stirring Methods 0.000 claims description 6
- 244000248557 Ophiopogon japonicus Species 0.000 claims description 5
- 241000222355 Trametes versicolor Species 0.000 claims description 5
- 238000012364 cultivation method Methods 0.000 claims description 5
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 3
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims description 3
- 235000011130 ammonium sulphate Nutrition 0.000 claims description 3
- 229940041514 candida albicans extract Drugs 0.000 claims description 3
- 239000001963 growth medium Substances 0.000 claims description 3
- 229940099596 manganese sulfate Drugs 0.000 claims description 3
- 239000011702 manganese sulphate Substances 0.000 claims description 3
- 235000007079 manganese sulphate Nutrition 0.000 claims description 3
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 claims description 3
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 3
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 3
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 claims description 3
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 claims description 3
- 239000011780 sodium chloride Substances 0.000 claims description 3
- 239000012138 yeast extract Substances 0.000 claims description 3
- 208000015181 infectious disease Diseases 0.000 claims 1
- 230000002458 infectious effect Effects 0.000 claims 1
- 241000238876 Acari Species 0.000 abstract description 14
- 239000002994 raw material Substances 0.000 abstract description 12
- 241000894006 Bacteria Species 0.000 abstract description 7
- 238000004519 manufacturing process Methods 0.000 abstract description 3
- 244000004281 Eucalyptus maculata Species 0.000 description 44
- 230000000052 comparative effect Effects 0.000 description 17
- 238000001994 activation Methods 0.000 description 13
- 229920000742 Cotton Polymers 0.000 description 10
- 238000012360 testing method Methods 0.000 description 6
- 239000002699 waste material Substances 0.000 description 6
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- 241000233866 Fungi Species 0.000 description 4
- 229940044949 eucalyptus oil Drugs 0.000 description 4
- 239000010642 eucalyptus oil Substances 0.000 description 4
- 244000085595 Zizania latifolia Species 0.000 description 3
- 235000004259 Zizania latifolia Nutrition 0.000 description 3
- CKUAXEQHGKSLHN-UHFFFAOYSA-N [C].[N] Chemical compound [C].[N] CKUAXEQHGKSLHN-UHFFFAOYSA-N 0.000 description 3
- 239000002361 compost Substances 0.000 description 3
- 210000003608 fece Anatomy 0.000 description 3
- 239000004615 ingredient Substances 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 229920002488 Hemicellulose Polymers 0.000 description 2
- 241000238631 Hexapoda Species 0.000 description 2
- 240000006677 Vicia faba Species 0.000 description 2
- 235000010749 Vicia faba Nutrition 0.000 description 2
- 235000002098 Vicia faba var. major Nutrition 0.000 description 2
- 241000607479 Yersinia pestis Species 0.000 description 2
- 230000003213 activating effect Effects 0.000 description 2
- 244000052616 bacterial pathogen Species 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 229920002678 cellulose Polymers 0.000 description 2
- 239000001913 cellulose Substances 0.000 description 2
- 239000010440 gypsum Substances 0.000 description 2
- 229910052602 gypsum Inorganic materials 0.000 description 2
- 229920002521 macromolecule Polymers 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 230000035764 nutrition Effects 0.000 description 2
- 230000035699 permeability Effects 0.000 description 2
- 238000005070 sampling Methods 0.000 description 2
- 239000010902 straw Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 235000017060 Arachis glabrata Nutrition 0.000 description 1
- 244000105624 Arachis hypogaea Species 0.000 description 1
- 235000010777 Arachis hypogaea Nutrition 0.000 description 1
- 235000018262 Arachis monticola Nutrition 0.000 description 1
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 241000255789 Bombyx mori Species 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 241000287828 Gallus gallus Species 0.000 description 1
- 229930195210 Ophiopogon Natural products 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- 244000098338 Triticum aestivum Species 0.000 description 1
- 235000004501 Volvariella volvacea Nutrition 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000003385 bacteriostatic effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000013401 experimental design Methods 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 239000003864 humus Substances 0.000 description 1
- 239000000077 insect repellent Substances 0.000 description 1
- 239000010871 livestock manure Substances 0.000 description 1
- 239000002068 microbial inoculum Substances 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 150000002772 monosaccharides Chemical class 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 235000020232 peanut Nutrition 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000007619 statistical method Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/50—Inoculation of spawn
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/60—Cultivation rooms; Equipment therefor
- A01G18/69—Arrangements for managing the environment, e.g. sprinklers
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/70—Harvesting
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Mycology (AREA)
- Environmental Sciences (AREA)
- Mushroom Cultivation (AREA)
Abstract
The invention relates to the technical field of straw mushroom cultivation, in particular to a method for cultivating straw mushroom by taking pure eucalyptus bark as a main material, wherein a cultivation material comprises the following components in percentage by mass: rice bran: the corn flour is 27-32: 2-6: 1, performing secondary fermentation on the cultivation material to cultivate straw mushrooms. According to the method for cultivating the straw mushrooms by using the pure eucalyptus barks as the main material, the eucalyptus barks are used as the main raw materials, so that the raw materials for cultivating the straw mushrooms are enriched, the comprehensive utilization rate of eucalyptus resources is improved, the production cost of the straw mushrooms is reduced, the quality and the biological efficiency of the straw mushrooms are improved, and the hazards of mites and mixed bacteria are reduced.
Description
Technical Field
The invention relates to the technical field of straw mushroom cultivation, in particular to a method for cultivating straw mushroom by taking pure eucalyptus bark as a main material.
Background
Volvaria volvacea (vollarella volvacea) is a high-temperature edible fungus and is widely cultivated in southern areas of China. Straw mushroom belongs to straw rotting fungi, hypha is mainly used for decomposing cellulose and hemicellulose in raw materials to enable the straw mushroom to become nutrition capable of being absorbed and utilized, and the traditional straw mushroom raw materials are waste cotton and cotton seed hulls. However, with the rapid increase of the prices of the cotton seed hulls and the waste cotton raw materials, people begin to use other materials to replace or partially replace the cotton seed hulls or the waste cotton to cultivate straw mushrooms. For example, CN103524248A, a method for preparing a straw mushroom cultivation material by using water bamboo sheathing leaves, which takes the water bamboo sheathing leaves as a main raw material and is supplemented with cotton seed hulls, cow dung, lime, gypsum powder and other auxiliary materials, the method stacks and ferments the water bamboo sheathing leaves, so that macromolecular substances are decomposed into substances which are beneficial to absorption of hyphae, the growth of the hyphae is promoted, meanwhile, the air permeability and the water retention property are strong, the straw mushroom fruiting is tidy, the yield is high, and the biological efficiency reaches 26.74% -30.4%. CN103420734A, a straw mushroom cultivation material compatibility and a preparation method of the cultivation material, the broad bean peel is used as a main raw material, and auxiliary materials such as fungus residue, cow dung, humus, gypsum powder, lime powder and the like are used as auxiliary materials, the broad bean peel is subjected to microbial fermentation, hypha takes in materials quickly, the growth is vigorous, the straw mushroom yield is high, and the biological efficiency reaches 35.2% -38.1%; there are also straw mushroom cultivation materials using eucalyptus waste, such as CN106069196A, a straw mushroom cultivation material, which uses eucalyptus waste, wheat straw and silkworm excrement as main raw materials, and chicken manure, peanut bran and other auxiliary materials as auxiliary materials for cultivating straw mushroom, but the auxiliary materials use too much organic matter and waste materials, which are easy to breed plant diseases and insect pests, such as: mites.
The information disclosed in this background section is only for enhancement of understanding of the general background of the invention and should not be taken as an acknowledgement or any form of suggestion that this information forms the prior art already known to a person skilled in the art.
Disclosure of Invention
The invention aims to provide a method for cultivating straw mushrooms by taking pure eucalyptus barks as main materials, which enriches the raw materials for cultivating straw mushrooms, improves the comprehensive utilization rate of eucalyptus resources, reduces the production cost of straw mushrooms, improves the quality and biological efficiency of straw mushrooms and reduces the damage of mites and mixed bacteria by taking the eucalyptus barks as the main raw materials.
In order to achieve the purpose, the invention provides the following technical scheme:
a straw mushroom cultivation material mainly comprising pure eucalyptus bark is composed of eucalyptus bark, rice bran and corn flour in a mass ratio, wherein the weight ratio of the eucalyptus bark: rice bran: the corn flour is 27-32: 2-6: 1.
preferably, the eucalyptus bark: rice bran: the corn flour is 29: 4: 1.
the invention also provides a method for cultivating straw mushrooms by using the straw mushroom cultivation material mainly containing the pure eucalyptus barks, which comprises the following steps:
(1) preparing a cultivation material according to the mass ratio, and crushing the eucalyptus bark into fine strips of the eucalyptus bark with the length of 0.2cm-0.3cm and the diameter of 0.1cm-0.5cm for later use; adding water with the mass of 18-25 times of the corn flour into the corn flour to dilute the corn flour into corn steep liquor for later use;
(2) and (3) first fermentation: inoculating microbial agent into the eucalyptus bark strips in an inoculation amount of 0.1-0.3%, adding water to adjust the water content to 60-65%, adding lime powder to adjust the pH to 8.5-9.0, and performing heap fermentation for 2-3 d; then adding rice bran and corn steep liquor, stirring to mix uniformly, adjusting the water content to 60-65% again, adjusting the pH to 8.5-9.0, piling and continuing to ferment for 3-4d to obtain an initial fermented material;
(3) and (3) secondary fermentation: adding water into the initial fermented material to adjust water content to 70% -75%, adding lime powder to adjust pH to 8.5-9.0, transferring to a fruiting room, spreading on a mushroom bed to loosen the fermented material, wherein the thickness of each layer is 10-15 cm; sealing the fruiting room, heating to 60-65 deg.C, maintaining for 4-8h, naturally cooling to 45 deg.C, and opening door and window;
(4) inoculation: when the temperature of the fermented material is reduced to 35-38 ℃, breaking the straw mushroom strains into blocks, uniformly scattering the blocks on the fermented material surface, compacting the material surface, and closing the door and window after inoculation is finished;
(5) management: the temperature of the fruiting room is kept at 28-35 ℃, the air humidity is 90-95%, and the light is 300 and 350 lux; opening doors and windows to ventilate for 0.5h in the morning, in the middle and at night every day at 6d after inoculation; spraying primary fruiting water when white spots with the size of rice grains appear on the material surface;
(6) harvesting: when the fruiting body of straw mushroom is egg-shaped and the mycoderm is not broken, harvesting in time.
Preferably, the microbial agent in the step (2) is prepared by mixing activated bacillus subtilis liquid and bacillus mucilaginosus liquid according to the mass ratio of 1: 2; the effective viable count of the bacillus subtilis liquid is 3.4 multiplied by 1010CUF/g-5.6×1010The effective viable count of the bacillus mucilaginosus liquid is 7.3 multiplied by 109CUF/g-9.1×109CUF/g。
Preferably, the activation method of the bacillus subtilis comprises the following steps: inoculating the bacillus subtilis strain into an activation medium I under the aseptic condition, and culturing for 16h at 35 ℃; the activation medium I comprises the following components: 7g of yeast extract, 15g of beef extract, 10g of peptone, 16g of coriolus versicolor polysaccharide, 2g of monopotassium phosphate, 1g of ammonium sulfate and 10ml of sterile water, wherein the initial pH is 7.4;
the activation method of the bacillus mucilaginosus comprises the following steps: inoculating the bacillus mucilaginosus strain into an activated culture medium II under the aseptic condition, and culturing for 12h at 35 ℃; the components of the activation medium II are as follows: 10g of beef extract, 15g of peptone, 18g of ophiopogon japonicus polysaccharide, 1g of manganese sulfate, 2g of sodium chloride and 15ml of sterile water, and the initial pH is 7.1.
The invention also provides the application of the straw mushroom cultivation material mainly prepared from the pure eucalyptus bark or the method for cultivating the straw mushroom in reducing the damage of mites and mixed bacteria in the straw mushroom cultivation process.
Compared with the prior art, the invention has the following beneficial effects:
(1) the raw material for cultivating the straw mushroom is mainly eucalyptus bark, so that eucalyptus bark resources are easy to obtain, the cost for cultivating the straw mushroom can be greatly reduced, and straw mushroom cultivators can obtain better economic benefit; meanwhile, the eucalyptus bark contains ingredients such as eucalyptus oil, so that the eucalyptus bark has a certain antibacterial property, and the volatile smell of the eucalyptus bark can also play a role in expelling insects; however, ingredients such as eucalyptus oil also inhibit the growth of hyphae, which affects the yield of edible fungi. Through the research of the inventor throughout the year, in the straw mushroom cultivation, if the cultivation material with high eucalyptus bark content is fermented twice and activated microbial agent is used in the fermentation process, ingredients such as eucalyptus oil and the like can be effectively degraded; the rice bran in the raw materials can ensure that the fermentation material has good permeability, and provides a relatively friendly environment for the growth of hypha; the carbon-nitrogen ratio of the eucalyptus bark is high, fermentation is not facilitated, the growth of mycelia is facilitated, the nitrogen content of the cultivation material can be increased by adding the corn flour, the overall carbon-nitrogen ratio is reduced, the requirement of straw mushroom mycelia on nitrogen nutrition is favorably met, and the yield of straw mushrooms is guaranteed.
(2) In the process of cultivating the straw mushrooms, the microbial inoculum is activated bacillus subtilis and bacillus mucilaginosus, and can promote macromolecular substances such as cellulose, hemicellulose, protein and the like in eucalyptus barks to be degraded into monosaccharide and amino acid which can be directly absorbed and utilized by the straw mushrooms, so that the biological efficiency is improved.
(3) The cultivation material is further decomposed through twice fermentation, and harmful substances are volatilized to avoid influencing the growth of hyphae; the second fermentation is carried out in the mushroom growing room, and the volatilized gas is matched with the high temperature in the fermentation process to kill the mixed bacteria and pests, especially mites, in the culture material and the mushroom growing room, so that the damage of the mites to the straw mushrooms is avoided.
Detailed Description
The present invention will be described in further detail with reference to specific examples, but the embodiments of the present invention are not limited to the scope of the examples. The activation method of bacillus subtilis described in the following examples is: inoculating the bacillus subtilis strain into an activation medium I under the aseptic condition, and culturing for 16h at 35 ℃; the activation medium I comprises the following components: 7g of yeast extract, 15g of beef extract, 10g of peptone, 16g of coriolus versicolor polysaccharide, 2g of monopotassium phosphate, 1g of ammonium sulfate and 10ml of sterile water, wherein the initial pH is 7.4;
the activation method of the bacillus mucilaginosus comprises the following steps: inoculating the bacillus mucilaginosus strain into an activated culture medium II under the aseptic condition, and culturing for 12h at 35 ℃; the components of the activation medium II are as follows: 10g of beef extract, 15g of peptone, 18g of ophiopogon japonicus polysaccharide, 1g of manganese sulfate, 2g of sodium chloride and 15ml of sterile water, and the initial pH is 7.1.
Example 1
A method for cultivating straw mushrooms by using straw mushroom cultivation material mainly containing pure eucalyptus barks comprises the following steps:
(1) preparing a cultivation material according to the mass ratio, namely eucalyptus bark: rice bran: the corn flour is 27: 2: 1, crushing eucalyptus bark into fine strips with the length of 0.2cm and the diameter of 0.1cm for later use; adding water with the mass being 18 times that of the corn flour into the corn flour to dilute the corn flour into corn steep liquor for later use;
(2) and (3) first fermentation: inoculating microbial agent into the eucalyptus bark strips in an inoculation amount of 0.1%, simultaneously adding water to adjust the water content to 60%, adding lime powder to adjust the pH to 8.5, and performing heap fermentation for 2 d; then adding rice bran and corn steep liquor, stirring to mix uniformly, adjusting the water content to 60% again, adjusting the pH to 8.5, building a pile, and continuing to ferment for 3d to obtain an initial fermented material;
(3) and (3) secondary fermentation: adding water into the initial fermented material to adjust water content to 70%, adding lime powder to adjust pH to 8.5, transferring to a mushroom-growing room, spreading on a mushroom bed to loosen the fermented material, wherein the thickness of each layer of material is 10 cm; sealing the fruiting room, heating to 60 deg.C, maintaining for 4 hr, naturally cooling to 45 deg.C, and opening the door and window;
(4) inoculation: when the temperature of the fermented material is reduced to 35 ℃, breaking the straw mushroom strains into blocks, uniformly scattering the blocks on the fermented material surface, compacting the material surface, and closing the door and window after inoculation is finished;
(5) management: the temperature of the fruiting room is kept at 28 ℃, the air humidity is 90 percent, and the light is 300 lux; opening doors and windows to ventilate for 0.5h in the morning, in the middle and at night every day at 6d after inoculation; spraying primary fruiting water when white spots with the size of rice grains appear on the material surface;
(6) harvesting: harvesting when the fruiting body of the straw mushroom is in an egg shape and the mycoderm is not broken in time;
wherein the microbial agent in the step (2) is prepared by mixing activated bacillus subtilis liquid and bacillus mucilaginosus liquid according to the mass ratio of 1: 2; the effective viable count of the bacillus subtilis liquid is 3.4 multiplied by 1010The effective viable count of the bacillus mucilaginosus liquid is 7.3 multiplied by 109CUF/g。
Example 2
A method for cultivating straw mushrooms by using straw mushroom cultivation material mainly containing pure eucalyptus barks comprises the following steps:
(1) preparing a cultivation material according to the mass ratio, namely eucalyptus bark: rice bran: the corn flour is 32: 6: 1, crushing eucalyptus bark into fine strips with the length of 0.3cm and the diameter of 0.5cm for later use; adding water with the mass of 25 times of the corn flour into the corn flour to dilute the corn flour into corn steep liquor for later use;
(2) and (3) first fermentation: inoculating microbial agent into the eucalyptus bark strips in an inoculation amount of 0.3%, simultaneously adding water to adjust the water content to 65%, adding lime powder to adjust the pH to 9.0, and performing heap fermentation for 3 d; then adding rice bran and corn steep liquor, stirring to mix uniformly, adjusting the water content to 65% again, adjusting the pH to 9.0, building a pile, and continuing to ferment for 4d to obtain an initial fermented material;
(3) and (3) secondary fermentation: adding water into the initial fermented material to adjust water content to 75%, adding lime powder to adjust pH to 9.0, transferring to a mushroom-growing room, spreading on a mushroom bed to loosen the fermented material, wherein the thickness of each layer is 15 cm; sealing the fruiting room, heating to 65 deg.C, maintaining for 8 hr, naturally cooling to 45 deg.C, and opening the door and window;
(4) inoculation: breaking the straw mushroom strains into blocks when the temperature of the fermented material is reduced to 38 ℃, uniformly scattering the blocks on the fermented material surface, compacting the material surface, and closing the door and window after inoculation;
(5) management: the fruiting room is kept at 35 ℃, the air humidity is 95%, and the light is 350 lux; opening doors and windows to ventilate for 0.5h in the morning, in the middle and at night every day at 6d after inoculation; spraying primary fruiting water when white spots with the size of rice grains appear on the material surface;
(6) harvesting: harvesting when the fruiting body of the straw mushroom is in an egg shape and the mycoderm is not broken in time;
wherein the microbial agent in the step (2) is prepared by mixing activated bacillus subtilis liquid and bacillus mucilaginosus liquid according to the mass ratio of 1: 2; the effective viable count of the bacillus subtilis liquid is 5.6 multiplied by 1010The effective viable count of the bacillus mucilaginosus liquid is 9.1 multiplied by 109CUF/g。
Example 3
A method for cultivating straw mushrooms by using straw mushroom cultivation material mainly containing pure eucalyptus barks comprises the following steps:
(1) preparing a cultivation material according to the mass ratio, namely eucalyptus bark: rice bran: the corn flour is 29: 4: 1, crushing eucalyptus bark into fine strips with the length of 0.3cm and the diameter of 0.5cm for later use; adding water with the mass of 21 times of the corn flour into the corn flour to dilute the corn flour into corn steep liquor for later use;
(2) and (3) first fermentation: inoculating microbial agent into the eucalyptus bark strips in an inoculation amount of 0.2%, simultaneously adding water to adjust the water content to 63%, adding lime powder to adjust the pH to 8.7, and performing heap fermentation for 3 d; then adding rice bran and corn steep liquor, stirring to mix uniformly, adjusting the water content to 63% again, adjusting the pH to 8.7, building a pile and continuing to ferment for 3d to obtain an initial fermented material;
(3) and (3) secondary fermentation: adding water into the initial fermented material to adjust water content to 73%, adding lime powder to adjust pH to 8.7, transferring to a mushroom growing room, spreading on a mushroom bed to loosen the fermented material, wherein the thickness of each layer of material is 13 cm; sealing the fruiting room, heating to 63 deg.C, maintaining for 6h, naturally cooling to 45 deg.C, and opening the door and window;
(4) inoculation: when the temperature of the fermented material is reduced to 36 ℃, breaking the straw mushroom strains into blocks, uniformly scattering the blocks on the fermented material surface, compacting the material surface, and closing the door and window after inoculation is finished;
(5) management: the fruiting room is kept at 31 ℃, the air humidity is 92% and the light is 325 lux; opening doors and windows to ventilate for 0.5h in the morning, in the middle and at night every day at 6d after inoculation; spraying primary fruiting water when white spots with the size of rice grains appear on the material surface;
(6) harvesting: harvesting when the fruiting body of the straw mushroom is in an egg shape and the mycoderm is not broken in time;
wherein the microbial agent in the step (2) is prepared by mixing activated bacillus subtilis liquid and bacillus mucilaginosus liquid according to the mass ratio of 1: 2; the effective viable count of the bacillus subtilis liquid is 4.5 multiplied by 1010The effective viable count of the bacillus mucilaginosus liquid is 8.2 multiplied by 109CUF/g。
Example 4
A method for cultivating straw mushrooms by using straw mushroom cultivation material mainly containing pure eucalyptus barks comprises the following steps:
(1) preparing a cultivation material according to the mass ratio, namely eucalyptus bark: rice bran: the corn flour is 29: 4: 1, crushing eucalyptus bark into fine strips with the length of 0.2cm and the diameter of 0.1cm for later use; adding water with the mass being 18 times that of the corn flour into the corn flour to dilute the corn flour into corn steep liquor for later use;
(2) and (3) first fermentation: inoculating microbial agent into the eucalyptus bark strips in an inoculation amount of 0.1%, simultaneously adding water to adjust the water content to 60%, adding lime powder to adjust the pH to 8.5, and performing heap fermentation for 2 d; then adding rice bran and corn steep liquor, stirring to mix uniformly, adjusting the water content to 60% again, adjusting the pH to 8.5, building a pile, and continuing to ferment for 3d to obtain an initial fermented material;
(3) and (3) secondary fermentation: adding water into the initial fermented material to adjust water content to 70%, adding lime powder to adjust pH to 8.5, transferring to a mushroom-growing room, spreading on a mushroom bed to loosen the fermented material, wherein the thickness of each layer of material is 10 cm; sealing the fruiting room, heating to 60 deg.C, maintaining for 4 hr, naturally cooling to 45 deg.C, and opening the door and window;
(4) inoculation: when the temperature of the fermented material is reduced to 35 ℃, breaking the straw mushroom strains into blocks, uniformly scattering the blocks on the fermented material surface, compacting the material surface, and closing the door and window after inoculation is finished;
(5) management: the temperature of the fruiting room is kept at 28 ℃, the air humidity is 90 percent, and the light is 300 lux; opening doors and windows to ventilate for 0.5h in the morning, in the middle and at night every day at 6d after inoculation; spraying primary fruiting water when white spots with the size of rice grains appear on the material surface;
(6) harvesting: harvesting when the fruiting body of the straw mushroom is in an egg shape and the mycoderm is not broken in time;
wherein the microbial agent in the step (2) is prepared by mixing activated bacillus subtilis liquid and bacillus mucilaginosus liquid according to the mass ratio of 1: 2; the effective viable count of the bacillus subtilis liquid is 3.4 multiplied by 1010The effective viable count of the bacillus mucilaginosus liquid is 7.3 multiplied by 109CUF/g。
Comparative example 1
The cultivation material of the comparative example does not add corn flour, the mass ratio of the eucalyptus bark to the rice bran is not changed, and other cultivation methods are the same as those of the example 1.
Comparative example 2
The eucalyptus bark of the cultivation material of the comparative example is replaced by the cotton seed hulls, and the other cultivation methods are the same as the example 1.
Comparative example 3
The activating medium I of the bacillus in the comparative example does not contain coriolus versicolor polysaccharide, the middle part of the activating medium II of the bacillus mucilaginosus contains ophiopogon polysaccharide, and other cultivation methods are the same as those in the example 1.
Comparative example 4
The cultivation material of this comparative example was not subjected to the second fermentation, and the other cultivation methods were the same as in example 1.
Test one: the fruiting condition of the straw mushroom cultivated by the invention
And (3) experimental design: 5 test groups of the working group 1, the comparative group 2, the comparative group 3 and the comparative group 4 were set. The implementation groups 1 to 3 are cultivated by the methods of the implementation groups 1 to 3 respectively; comparative groups 1 to 4 were cultivated by the methods of comparative examples 1 to 4, respectively.
Each test group is provided with 40 cells, each cell is 1.5m2Marking, observing and counting the occurrence conditions of mites and mixed bacteria respectively at 3d and 7d after fruiting, counting the yield of the straw mushrooms in unit area of each test group after mature harvest, and calculating the biological efficiency.
The statistical method of the occurrence condition of the mites comprises the following steps: randomly selecting 20 cells in each test group, sampling at five points in each cell, taking 100g of fermentation material with the depth of 0-5cm from each sampling point, flatly spreading the taken fermentation material, observing and recording the quantity of mites by using a hand-held magnifier, calculating the quantity of mites in each hundred grams of mass, and obtaining the test results shown in Table 1.
Yield of straw mushroom (kg/m)2) Weight of harvested straw mushroom divided by total area;
biological efficiency (%) (weight of harvested straw mushroom ÷ dry amount of compost) × 100;
the incidence of mixed germs (%) (number of cells where mixed germs appear ÷ total number of cells) × 100;
the number of mites (strips/100 g) ═ the number of mites ÷ (total mass of fermented material sampled) × 100;
TABLE 1 fruiting of the invention for cultivation of volvariella volvacea
As can be seen from Table 1, in the example 1, compared with the comparative example 1, the carbon-nitrogen ratio of the whole corn meal-lacking compost is higher, so that the growth of hyphae is not facilitated, and the biological efficiency of the compost is reduced; compared with the comparative example 2, the eucalyptus bark is replaced by the cotton seed hulls, although the secondary fermentation is carried out, a small amount of mites and mixed bacteria still appear in the growth process of the straw mushrooms, because the cotton seed hulls do not contain bacteriostatic and insect-repellent components; compared with the comparative example 3, in the embodiment 1, when the coriolus versicolor polysaccharide in the activation medium I and the ophiopogon japonicus polysaccharide in the activation medium II are absent, the production of extracellular enzymes in the activation process of the microorganisms is influenced, so that the degradation of culture materials is influenced, the biological efficiency is reduced, and the yield of the straw mushrooms is reduced; compared with the comparative example 4, the eucalyptus oil in the eucalyptus bark can not be degraded well only by one-time fermentation, and mites and other bacteria can grow in the culture material.
The foregoing descriptions of specific exemplary embodiments of the present invention have been presented for purposes of illustration and description. It is not intended to limit the invention to the precise form disclosed, and obviously many modifications and variations are possible in light of the above teaching. The exemplary embodiments were chosen and described in order to explain certain principles of the invention and its practical application to enable one skilled in the art to make and use various exemplary embodiments of the invention and various alternatives and modifications as are suited to the particular use contemplated. It is intended that the scope of the invention be defined by the claims and their equivalents.
Claims (3)
1. A straw mushroom cultivation method is characterized by comprising the following steps:
(1) preparing a cultivation material according to the mass ratio, and crushing the eucalyptus bark into fine strips of the eucalyptus bark with the length of 0.2cm-0.3cm and the diameter of 0.1cm-0.5cm for later use; adding water with the mass of 18-25 times of the corn flour into the corn flour to dilute the corn flour into corn steep liquor for later use;
(2) and (3) first fermentation: inoculating microbial agent into the eucalyptus bark strips in an inoculation amount of 0.1-0.3%, adding water to adjust the water content to 60-65%, adding lime powder to adjust the pH to 8.5-9.0, and performing heap fermentation for 2-3 d; then adding rice bran and corn steep liquor, stirring to mix uniformly, adjusting the water content to 60-65% again, adjusting the pH to 8.5-9.0, piling and continuing to ferment for 3-4d to obtain an initial fermented material;
(3) and (3) secondary fermentation: adding water into the initial fermented material to adjust water content to 70% -75%, adding lime powder to adjust pH to 8.5-9.0, transferring to a fruiting room, spreading on a mushroom bed to loosen the fermented material, wherein the thickness of each layer is 10-15 cm; sealing the fruiting room, heating to 60-65 deg.C, maintaining for 4-8h, naturally cooling to 45 deg.C, and opening door and window;
(4) inoculation: when the temperature of the fermented material is reduced to 35-38 ℃, breaking the straw mushroom strains into blocks, uniformly scattering the blocks on the fermented material surface, compacting the material surface, and closing the door and window after inoculation is finished;
(5) management: the temperature of the fruiting room is kept at 28-35 ℃, the air humidity is 90-95%, and the light is 300 and 350 lux; opening doors and windows to ventilate for 0.5h in the morning, in the middle and at night every day at 6d after inoculation; spraying primary fruiting water when white spots with the size of rice grains appear on the material surface;
(6) harvesting: harvesting when the fruiting body of the straw mushroom is in an egg shape and the mycoderm is not broken in time;
the cultivation material comprises eucalyptus bark, rice bran and corn flour in a mass ratio, wherein the eucalyptus bark: rice bran: the corn flour is 27-32: 2-6: 1;
the microbial agent in the step (2) is prepared by mixing activated bacillus subtilis liquid and bacillus mucilaginosus liquid according to the mass ratio of 1: 2; the effective viable count of the bacillus subtilis liquid is 3.4 multiplied by 1010CUF/g-5.6×1010The effective viable count of the bacillus mucilaginosus liquid is 7.3 multiplied by 109CUF/g-9.1×109CUF/g;
The activation method of the bacillus subtilis comprises the following steps: inoculating the bacillus subtilis strain into an activation medium I under the aseptic condition, and culturing for 16h at 35 ℃; the activation medium I comprises the following components: 7g of yeast extract, 15g of beef extract, 10g of peptone, 16g of coriolus versicolor polysaccharide, 2g of monopotassium phosphate, 1g of ammonium sulfate and 10ml of sterile water, wherein the initial pH is 7.4;
the activation method of the bacillus mucilaginosus comprises the following steps: inoculating the bacillus mucilaginosus strain into an activated culture medium II under the aseptic condition, and culturing for 12h at 35 ℃; the components of the activation medium II are as follows: 10g of beef extract, 15g of peptone, 18g of ophiopogon japonicus polysaccharide, 1g of manganese sulfate, 2g of sodium chloride and 15ml of sterile water, and the initial pH is 7.1.
2. The method for cultivating straw mushroom according to claim 1, wherein the eucalyptus bark: rice bran: the corn flour is 29: 4: 1.
3. use of a method of straw mushroom cultivation according to any one of claims 1-2 for reducing mite and infectious microbe hazards during straw mushroom cultivation.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201910485417.4A CN110226455B (en) | 2019-06-05 | 2019-06-05 | Method for cultivating straw mushroom by using pure eucalyptus bark as main material |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201910485417.4A CN110226455B (en) | 2019-06-05 | 2019-06-05 | Method for cultivating straw mushroom by using pure eucalyptus bark as main material |
Publications (2)
Publication Number | Publication Date |
---|---|
CN110226455A CN110226455A (en) | 2019-09-13 |
CN110226455B true CN110226455B (en) | 2021-08-17 |
Family
ID=67858444
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201910485417.4A Active CN110226455B (en) | 2019-06-05 | 2019-06-05 | Method for cultivating straw mushroom by using pure eucalyptus bark as main material |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN110226455B (en) |
Citations (13)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102498930A (en) * | 2011-10-13 | 2012-06-20 | 成都榕珍菌业有限公司 | Method for cultivating straw mushrooms by utilizing pleurotus eryngii fungi residues |
CN104591871A (en) * | 2014-12-26 | 2015-05-06 | 成都新柯力化工科技有限公司 | Ecological potassium fertilizer and preparation method thereof |
CN105325174A (en) * | 2015-11-25 | 2016-02-17 | 苏州市经纬农产品有限公司 | Cultivation method of straw mushrooms |
CN105646037A (en) * | 2016-01-05 | 2016-06-08 | 安徽霍山县草之灵中药材有限公司 | Preparation method of dendrobium officinale planting fertilizer |
CN106069196A (en) * | 2016-06-30 | 2016-11-09 | 广西仁泰生物科技有限公司 | A kind of method utilizing Eucalyptus waste material and wheat straw to produce Volvariella volvacea (Bull.Ex Franch.) Singer. |
CN106171509A (en) * | 2016-06-30 | 2016-12-07 | 广西仁泰生物科技有限公司 | A kind of method utilizing Eucalyptus waste material and Caulis et Folium Oryzae to produce Volvariella volvacea (Bull.Ex Franch.) Singer. |
CN106455494A (en) * | 2014-05-27 | 2017-02-22 | 诺维信公司 | Methods for mushroom cultivation |
CN106718075A (en) * | 2017-03-10 | 2017-05-31 | 铜仁职业技术学院 | A kind of cultivation technique of straw mushroom new varieties |
CN106747935A (en) * | 2016-11-24 | 2017-05-31 | 凤台县兰韵食用菌专业合作社 | A kind of selenium-rich Volvaria volvacea cultivation material and its cultural method |
CN107602179A (en) * | 2017-08-23 | 2018-01-19 | 广西金穗生态科技股份有限公司 | Eucalyptus barks recycling high-value utilization process |
CN107619316A (en) * | 2017-08-23 | 2018-01-23 | 广西金穗生态科技股份有限公司 | The preparation method of dragon fruit plantation half matrix fertilizer |
CN109392605A (en) * | 2018-12-12 | 2019-03-01 | 广西壮族自治区农业科学院微生物研究所 | A method of with the cultivating bisporous mushroom of eucalyptus barks |
CN109526551A (en) * | 2018-12-12 | 2019-03-29 | 广西壮族自治区农业科学院微生物研究所 | A kind of cultural method of natural selenium-rich agaricus bisporus |
-
2019
- 2019-06-05 CN CN201910485417.4A patent/CN110226455B/en active Active
Patent Citations (13)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102498930A (en) * | 2011-10-13 | 2012-06-20 | 成都榕珍菌业有限公司 | Method for cultivating straw mushrooms by utilizing pleurotus eryngii fungi residues |
CN106455494A (en) * | 2014-05-27 | 2017-02-22 | 诺维信公司 | Methods for mushroom cultivation |
CN104591871A (en) * | 2014-12-26 | 2015-05-06 | 成都新柯力化工科技有限公司 | Ecological potassium fertilizer and preparation method thereof |
CN105325174A (en) * | 2015-11-25 | 2016-02-17 | 苏州市经纬农产品有限公司 | Cultivation method of straw mushrooms |
CN105646037A (en) * | 2016-01-05 | 2016-06-08 | 安徽霍山县草之灵中药材有限公司 | Preparation method of dendrobium officinale planting fertilizer |
CN106171509A (en) * | 2016-06-30 | 2016-12-07 | 广西仁泰生物科技有限公司 | A kind of method utilizing Eucalyptus waste material and Caulis et Folium Oryzae to produce Volvariella volvacea (Bull.Ex Franch.) Singer. |
CN106069196A (en) * | 2016-06-30 | 2016-11-09 | 广西仁泰生物科技有限公司 | A kind of method utilizing Eucalyptus waste material and wheat straw to produce Volvariella volvacea (Bull.Ex Franch.) Singer. |
CN106747935A (en) * | 2016-11-24 | 2017-05-31 | 凤台县兰韵食用菌专业合作社 | A kind of selenium-rich Volvaria volvacea cultivation material and its cultural method |
CN106718075A (en) * | 2017-03-10 | 2017-05-31 | 铜仁职业技术学院 | A kind of cultivation technique of straw mushroom new varieties |
CN107602179A (en) * | 2017-08-23 | 2018-01-19 | 广西金穗生态科技股份有限公司 | Eucalyptus barks recycling high-value utilization process |
CN107619316A (en) * | 2017-08-23 | 2018-01-23 | 广西金穗生态科技股份有限公司 | The preparation method of dragon fruit plantation half matrix fertilizer |
CN109392605A (en) * | 2018-12-12 | 2019-03-01 | 广西壮族自治区农业科学院微生物研究所 | A method of with the cultivating bisporous mushroom of eucalyptus barks |
CN109526551A (en) * | 2018-12-12 | 2019-03-29 | 广西壮族自治区农业科学院微生物研究所 | A kind of cultural method of natural selenium-rich agaricus bisporus |
Non-Patent Citations (5)
Title |
---|
利用杏鲍菇废菌渣栽培草菇;王财富;《食药用菌》;20110331(第02期);第33-34页 * |
广西农作物秸秆基料化生产食用菌现状及对策研究;黄奕洲等;《广西农学报》;20170820(第04期);第29-32页 * |
稻草床栽草菇复棉籽壳能增产;涂改临;《中国食用菌》;19881027(第05期);第30-31页 * |
草菇高产优质栽培技术;林进路;《食用菌》;20180123(第01期);第52-53页 * |
龙海市草菇室内床架式栽培技术;蔡志英;《食用菌》;20081123(第06期);第44-45页 * |
Also Published As
Publication number | Publication date |
---|---|
CN110226455A (en) | 2019-09-13 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN101759494B (en) | Diseases-prevention organic fertilizer prepared by edible fungi residue | |
CN102617208B (en) | Edible fungus residue organic fertilizer raw materials and preparation method thereof | |
CN109618944B (en) | Hypersensitive protein complex enzyme preparation as well as preparation method and application thereof | |
CN106396807A (en) | Culture medium for improving bioconversion rate of edible fungi, preparation method of culture medium and cultivation method of edible fungi | |
CN106190862B (en) | Mushroom dreg fermentation substrate, morchella strain culture substrate and preparation method thereof | |
CN101786911A (en) | Microorganism and enzyme powder composite biological agent for microbial organic fertilizer | |
CN104193516A (en) | Environment-friendly fertilizer for strawberries and preparation method thereof | |
CN109679860A (en) | A kind of composite bacteria agent and the preparation method and application thereof for the processing of gardens green waste | |
CN106187515B (en) | Utilize the hickory chick nutrient bag and preparation method thereof of edible fungi residue production | |
CN103382134A (en) | Fermentation method of Agaricus bisporus (Lange) Sing cultivation matrix | |
CN103382139A (en) | Agaricus bisporus (Lange) Sing culture medium | |
CN101759493B (en) | Biological disease-preventing organic fertilizer and preparation method thereof | |
CN101897272B (en) | Integral resource recycling method of bagasse | |
CN107652093A (en) | A kind of preparation method of masson pine seedling medium | |
CN108203352A (en) | A kind of production method of kitchen garbage organic fertilizer | |
CN101885640A (en) | Method for preparing mushroom medium by using microbial composite bacteria | |
CN107473791A (en) | Planting almond abalone mushroom matrix | |
CN112021073A (en) | Morchella esculenta external aid nutrition bag ingredient, nutrition bag and preparation method thereof | |
CN112321338A (en) | Biological organic fertilizer | |
CN103351188B (en) | Preparation method of agricultural organic fertilizer by treating pure straws as raw material | |
CN105724055B (en) | A method of improving agaricus bisporus yield using needle mushroom dreg | |
CN105037045B (en) | A kind of soybean nutritional liquid and preparation method thereof and application method | |
CN101288359A (en) | Method for breaking hard seed dormancy of alfalfa seeds | |
CN109392605B (en) | Method for cultivating agaricus bisporus by using eucalyptus bark | |
CN110637676A (en) | Termitomyces albuminosus cultivation method and cultivation medium |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
GR01 | Patent grant | ||
GR01 | Patent grant | ||
TR01 | Transfer of patent right | ||
TR01 | Transfer of patent right |
Effective date of registration: 20220421 Address after: No.174, Daxue East Road, Nanning, Guangxi 530003 Patentee after: GUANGXI ACADEMY OF AGRICULTURAL SCIENCES Address before: No.174, Daxue East Road, XiXiangTang District, Nanning City, Guangxi Zhuang Autonomous Region Patentee before: INSTITUTE OF MICROBIOLOGY, GUANGXI ZHUANG AUTONOMOUS ACADEMY OF AGRICULTURAL SCIENCES |