CN110199697A - A kind of method of endangered plants hopea chinensis Water culture breeding - Google Patents
A kind of method of endangered plants hopea chinensis Water culture breeding Download PDFInfo
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- CN110199697A CN110199697A CN201910569144.1A CN201910569144A CN110199697A CN 110199697 A CN110199697 A CN 110199697A CN 201910569144 A CN201910569144 A CN 201910569144A CN 110199697 A CN110199697 A CN 110199697A
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- Prior art keywords
- hopea
- cuttings
- cuttage
- culture
- chinensis
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Links
- 241000086827 Hopea chinensis Species 0.000 title claims abstract description 40
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title claims abstract description 38
- 238000000034 method Methods 0.000 title claims abstract description 30
- 238000009395 breeding Methods 0.000 title claims abstract description 22
- 230000001488 breeding effect Effects 0.000 title claims abstract description 20
- 238000005520 cutting process Methods 0.000 claims abstract description 89
- 235000015097 nutrients Nutrition 0.000 claims abstract description 35
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims abstract description 18
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 17
- 238000012258 culturing Methods 0.000 claims abstract description 13
- 239000002023 wood Substances 0.000 claims abstract description 9
- 235000016709 nutrition Nutrition 0.000 claims abstract description 6
- 239000000287 crude extract Substances 0.000 claims abstract description 5
- 230000035764 nutrition Effects 0.000 claims abstract description 5
- 239000000243 solution Substances 0.000 claims description 32
- 238000004659 sterilization and disinfection Methods 0.000 claims description 14
- 239000007864 aqueous solution Substances 0.000 claims description 13
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 12
- 238000004140 cleaning Methods 0.000 claims description 12
- 238000002791 soaking Methods 0.000 claims description 12
- 238000013329 compounding Methods 0.000 claims description 10
- 241000196324 Embryophyta Species 0.000 claims description 8
- 241000607479 Yersinia pestis Species 0.000 claims description 7
- 201000010099 disease Diseases 0.000 claims description 7
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 7
- 239000007788 liquid Substances 0.000 claims description 7
- 230000008774 maternal effect Effects 0.000 claims description 7
- 230000003287 optical effect Effects 0.000 claims description 7
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 6
- 229910000831 Steel Inorganic materials 0.000 claims description 6
- 238000004378 air conditioning Methods 0.000 claims description 6
- 230000002146 bilateral effect Effects 0.000 claims description 6
- 238000004851 dishwashing Methods 0.000 claims description 6
- 239000011521 glass Substances 0.000 claims description 6
- 238000007654 immersion Methods 0.000 claims description 6
- 229920003023 plastic Polymers 0.000 claims description 6
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 claims description 6
- 239000010959 steel Substances 0.000 claims description 6
- 229920000742 Cotton Polymers 0.000 claims description 5
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 5
- 239000012153 distilled water Substances 0.000 claims description 5
- 238000011010 flushing procedure Methods 0.000 claims description 5
- XLYOFNOQVPJJNP-ZSJDYOACSA-N heavy water Substances [2H]O[2H] XLYOFNOQVPJJNP-ZSJDYOACSA-N 0.000 claims description 5
- 229940079593 drug Drugs 0.000 claims description 4
- 239000003814 drug Substances 0.000 claims description 4
- 238000012545 processing Methods 0.000 claims description 4
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 3
- 229910021580 Cobalt(II) chloride Inorganic materials 0.000 claims description 3
- 239000007836 KH2PO4 Substances 0.000 claims description 3
- 229910004619 Na2MoO4 Inorganic materials 0.000 claims description 3
- PVNIIMVLHYAWGP-UHFFFAOYSA-N Niacin Chemical compound OC(=O)C1=CC=CN=C1 PVNIIMVLHYAWGP-UHFFFAOYSA-N 0.000 claims description 3
- 239000002253 acid Substances 0.000 claims description 3
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 claims description 3
- 239000001110 calcium chloride Substances 0.000 claims description 3
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 3
- 229910052564 epsomite Inorganic materials 0.000 claims description 3
- 229940064880 inositol 100 mg Drugs 0.000 claims description 3
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 claims description 3
- 229910000357 manganese(II) sulfate Inorganic materials 0.000 claims description 3
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 3
- 235000001968 nicotinic acid Nutrition 0.000 claims description 3
- 229960003512 nicotinic acid Drugs 0.000 claims description 3
- 239000011664 nicotinic acid Substances 0.000 claims description 3
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 claims description 3
- 150000003839 salts Chemical class 0.000 claims description 3
- 239000011684 sodium molybdate Substances 0.000 claims description 3
- TVXXNOYZHKPKGW-UHFFFAOYSA-N sodium molybdate (anhydrous) Chemical compound [Na+].[Na+].[O-][Mo]([O-])(=O)=O TVXXNOYZHKPKGW-UHFFFAOYSA-N 0.000 claims description 3
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 claims description 3
- 229960000344 thiamine hydrochloride Drugs 0.000 claims description 3
- 235000019190 thiamine hydrochloride Nutrition 0.000 claims description 3
- 239000011747 thiamine hydrochloride Substances 0.000 claims description 3
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 claims description 3
- 229910000368 zinc sulfate Inorganic materials 0.000 claims description 3
- 239000011686 zinc sulphate Substances 0.000 claims description 3
- 238000003780 insertion Methods 0.000 claims description 2
- 230000037431 insertion Effects 0.000 claims description 2
- 230000008439 repair process Effects 0.000 claims description 2
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 claims 1
- 229910000366 copper(II) sulfate Inorganic materials 0.000 claims 1
- 239000002574 poison Substances 0.000 claims 1
- 231100000614 poison Toxicity 0.000 claims 1
- 238000005406 washing Methods 0.000 claims 1
- 230000002401 inhibitory effect Effects 0.000 abstract description 2
- 239000012530 fluid Substances 0.000 abstract 1
- 239000011159 matrix material Substances 0.000 description 14
- 230000000052 comparative effect Effects 0.000 description 6
- 239000002689 soil Substances 0.000 description 6
- 239000000356 contaminant Substances 0.000 description 5
- 238000012549 training Methods 0.000 description 5
- 229920002457 flexible plastic Polymers 0.000 description 4
- 241000894007 species Species 0.000 description 4
- 230000004083 survival effect Effects 0.000 description 4
- 238000009966 trimming Methods 0.000 description 4
- 238000009826 distribution Methods 0.000 description 3
- 239000007787 solid Substances 0.000 description 3
- 241000123611 Dipterocarpaceae Species 0.000 description 2
- 241000907895 Hopea Species 0.000 description 2
- AFCARXCZXQIEQB-UHFFFAOYSA-N N-[3-oxo-3-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)propyl]-2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidine-5-carboxamide Chemical compound O=C(CCNC(=O)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F)N1CC2=C(CC1)NN=N2 AFCARXCZXQIEQB-UHFFFAOYSA-N 0.000 description 2
- 229910052927 chalcanthite Inorganic materials 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000003415 peat Substances 0.000 description 2
- 239000010455 vermiculite Substances 0.000 description 2
- 229910052902 vermiculite Inorganic materials 0.000 description 2
- 235000019354 vermiculite Nutrition 0.000 description 2
- 238000013459 approach Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 230000007797 corrosion Effects 0.000 description 1
- 238000005260 corrosion Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000011121 hardwood Substances 0.000 description 1
- 238000003973 irrigation Methods 0.000 description 1
- 230000002262 irrigation Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000004899 motility Effects 0.000 description 1
- 239000002420 orchard Substances 0.000 description 1
- 230000002688 persistence Effects 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 238000013138 pruning Methods 0.000 description 1
- 238000004080 punching Methods 0.000 description 1
- 230000007115 recruitment Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000004576 sand Substances 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 description 1
- 238000009333 weeding Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/10—Vegetative propagation by means of cuttings
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
- C05G5/23—Solutions
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Environmental Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Botany (AREA)
- Developmental Biology & Embryology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention discloses a kind of methods of endangered plants hopea chinensis Water culture breeding, 1~2 year raw stiff wood item is acquired as cuttings, 1~2h is impregnated in the GGR root-growing agent of 600~800mg/L, it is inserted into cuttage plate, it is placed in the culture vessel equipped with nutrient solution, culture vessel is placed on culture on culturing rack and is managed and protected until taking root.The special nutrient fluid that the present invention prepares can effectively promote hopea chinensis cuttage root-taking, and hopea chinensis methanol crude extract is added in nutrient solution, to mould have certain inhibiting effect, efficiently avoid it is mouldy cause fringe bar rot, rooting rate is fast, rooting rate is high and stablizes, using nutrient solution as medium water planting cuttage and seedling culture, ambient stable is controllable, and nutrition is balanced, seedling growth is healthy and strong consistent, management is easy, can save great amount of cost, has good application value and realistic meaning.
Description
Technical field
The invention belongs to achievements in forest-tree seedling Cultivating techniques field, it is related to a kind of endangered plants hopea chinensis Water culture breeding
Method.
Background technique
Hopea chinensis (Hopea chinensis Hand.-Mazz.) is under the jurisdiction of Dipterocarpaceae (Dipterocarpaceae)
Hopea (Hopea), aiphyllium, be the peculiar rare and endangered tree species in Guangxi, Gui Nanbei tropical evergreen monsoon forest represent tree species it
One, research tropical rain forest fauna is constituted, Distribution and ecology habit has important value.Meanwhile material is hard, corrosion resistant
Power is extremely strong, referred to as " Wan Nianmu ", can be used as the use such as military project, machinery and noble furniture, bark has imperial robe shape decorative pattern, therefore also has
The title of " imperial robe tree ".Hopea chinensis belongs to the relict in the Tertiary Period, is distributed in local landform and is formed by " sanctuary ", original point
It is distributed in Shiwandashan, Guangxi and Daqunshan Mountains height above sea level 600m the foot of a mountain below, cheuch, in the woods of hillside.But due to human production activity's
Destroy and the influence of climatic variation, the wild hopea chinensis in Daqunshan Mountains has become history, at present only Shiwan Dashan from
There are also a small amount of wild hopea chinensis distribution in right protection zone, is divided into first-grade state protection plant, joined by world's conservation of nature
Alliance (IUCN) is classified as pole danger (CR) species.
The key of rescue endangered plants is to expand population scale.Wild hopea chinensis is distributed in by trench more, population quantity
Rareness, natural propagation is difficult, and usually flowering asynchronism causes its seed amount few, and seed is fallen in streams more, it is difficult to it sprouts,
Population recruitment speed is slow.Extremely narrow distribution and minimum species quantity are badly in need of meeting protection by new reproduction technique
With expand the needs of hopea chinensis resource, and explore the approach of germ plasm resource strange land preservation, ensure the excellent hereditary base of hopea chinensis
The persistence of cause.Cutting propagation can it is economical, effectively utilize propagation material, it is preferable to save maternal hereditary capacity, behaviour
Make simply, at low cost, growing-seedling period is short, and reproduction speed is fast.But traditional cuttage technique fringe bar is required it is stringent, hardwood cutting at
Motility rate is lower, a large amount of artificial construction cutting orchards need to be put into, so that seedling cost increases；And cuttage survival rate is managed, oneself
The multifactor impacts such as right environment are larger, and survival rate stability is not strong, ineffective；In addition, traditional cuttage breeding method is with soil
Earth, fine sand etc. are used as matrix, it is difficult to observe its condition of rooting and control the nutrition-allocated proportion after cuttage.
Water culture is to replace common matrix with water or nutrient solution, solves with soil, buys the problems such as basin and irrigation management, has
Have the advantages that efficient and convenient, low in cost, Yi Guanyang, clean pollution-free, low-carbon environment-friendly, limit by natural conditions it is small, simultaneously and also
Nutritional ingredient can accurately be controlled, be conducive to the upgrowth situation for understanding cutting, modern produce can be suitble in a large amount of nursery of more seasons
Industryization is cultivated.
Summary of the invention
The purpose of the present invention is overcome the deficiencies of the prior art and provide a kind of efficiently convenient, easy to operate, root survival
The method for being stable and easy to the hopea chinensis Water culture promoted breeding, is quickly obtained a large amount of hopea chinensis nursery stock.
To achieve the goals above, the technical solution adopted by the present invention is that:
A kind of method of endangered plants hopea chinensis Water culture breeding, prepares, compounding comprising sticking device, inserts
Fringe acquisition and processing, root-growing agent impregnate, cuttage and insert after manage, key step is as follows:
(1) sticking device prepares: selecting length × width × height is respectively 35.2cm × 25cm × 9.5cm plastic casing as training
Container is supported, uses the pearl cotton plate of 1.5~2.5cm thickness as cuttage plate, is built with angle steel and grow 180~200cm, wide by 80~
5 layers of glass plate of 100cm are placed on spare in the room equipped with curtain, air-conditioning and humidifier as culturing rack after disinfection；
(2) compounding: nutrient solution is prepared according to formula as below, the hopea chinensis methanol that 10000mg/L is added slightly mentions
Object, setting up pH using 1mol/L NaOH and hydrochloric acid is 5.8~6.0, is stored in 4 DEG C of refrigerators；
| KNO3 | 950mg/L |
| NH4NO3 | 825mg/L |
| KH2PO4 | 85mg/L |
| CaCl2·2H2O | 220mg/L |
| MgSO4·7H2O | 185mg/L |
| MnSO4·6H2O | 22.3mg/L |
| ZnSO4·7H2O | 8.6mg/L |
| CuSO4·5H2O | 0.025mg/L |
| H3BO3 | 6.2mg/L |
| Na2MoO4·2H2O | 0.25mg/L |
| CoCl2·6H2O | 0.025mg/L |
| Thiamine hydrochloride | 10mg/L |
| Puridoxine hydrochloride | 0.5mg/L |
| Niacin | 0.5mg/L |
| Inositol | 100mg/L |
| Na2Fe-EDTA | 28mg/L |
| IBA | 1.5mg/L |
| IAA | 0.5mg/L |
(3) cuttings acquires: selecting robust growth, the select tree without disease pest as maternal plant, acquires stiff wood item as cuttings item, repair
It cuts, clean, sterilizing and obtain cuttings；
(4) root-growing agent impregnates: cuttings being soaked in root-growing agent, soaking depth is 2.5~4.5cm；
(5) cuttage: the cuttings oblique cutting that immersion treatment is crossed is entered in the hole accomplished fluently on cuttage plate, is poured into culture vessel
The nutrient solution prepared, nutrient solution highest interface distance culture vessel 1~3cm of edge, cuttage plate is put into nutrient solution；
(6) it is managed after inserting: after inserting post-tensioning good curtain darkroom culture 1d, drawing the curtain apart using nature optical culture, remain indoor
20~26 DEG C of temperature, humidity 80~90%, 7~10d replaces the nutrient solution being equally formulated；
Further, the specification length and width of step (1) the cuttage plate are respectively less than culture vessel 3~5cm of length and width, and with punching
Device beats cutting hole, and cuttage aperture is 0.3~0.5cm, 3~5cm of interval of each cutting hole.
Further, step (1) disinfection is using 1:24 bromogeramine aqueous solution wiping culture vessel, cuttage plate and training
Support frame surface.
Further, it is mixed again after need individually dissolving each drug when step (2) described compounding.
Further, the diameter of step (3) the cuttings item is 0.25~0.45cm, 1~2 year raw stiff wood item.
Further, step (3) described pruning method is to retain cutting length for 8.5~12.5cm, the side shoot 0.5 of bilateral
~0.8cm does not retain leaf, and plane is cut into upper end, and inclined-plane is cut into lower end, and cutting angle and horizontal sextant angle are 60 °.
Further, step (3) described cleaning method is to be with volume fraction with soft bristle tooth brush light brush cuttings surface
0.08% dish washing liquid aqueous cleaning cuttings surface contaminants；It is described disinfection using 75% alcohol impregnate 1~3min, then with distill
Water rinses 3~5 times.
Further, step (4) root-growing agent impregnates the aqueous solution with 600~800mg/L GGR, soaking time 1
~2h.
Further, it is 2~4cm that step (5) described cottage method, which is by cuttings insertion cuttage plate posterior end exposed length,
Cuttings lower end exposed portion is kept to be completely immersed in nutrient solution.
The present invention compared with prior art the advantages of and beneficial effect be:
(1) cuttage place of the present invention carries out indoors, can effectively be controlled environment, and breeding is not limited by season
System, at the same apply cuttage culturing rack, can the sufficiently effective space using place, save a large amount of places, being capable of industrial scale
Change and carries out hopea chinensis breeding.
(2) present invention selects pearl cotton plate as cuttage plate, both will not be because during cuttage with certain elasticity
Cause greatly very much to be not fixed firmly for hole, will not cause to damage cuttings during cuttage because of hole is too small.
(3) excellent nutrient solution dedicated, full of nutrition is bred according to having prepared hopea chinensis Water culture, it can be to nutrition
Ingredient accurately controls, and effectively facilitates hopea chinensis cuttage root-taking, and hopea chinensis methanol crude extract is added in nutrient solution,
To mould have certain inhibiting effect, efficiently avoid it is mouldy cause cuttings rot.
(4) 1d is cultivated in darkroom after the present invention inserts, and then uses nature optical culture, maintains 20~26 DEG C of room temperature, humidity
80~90%, 7~10d replace the nutrient solution being equally formulated, and are conducive to the breeding of hopea chinensis Water culture.
(5) start to take root after cuttage 10d of the present invention, rooting rate is up to 90% or more after 45d, the healthy and strong flourishing, speed of taking root of root system
Degree is fast, rooting rate is high and stablizes, and breeds the about 15d ahead of time that starts to take root than common soil matrix matter, rooting rate improves 17% after 45d, greatly
The cuttage and seedling culture period is shortened greatly.
(6) cutting propagation of the present invention uses nutrient solution for medium aqueous solution, controllable to ambient stable, and nutrition is balanced, nursery stock
Robust growth is consistent, and management is easy, it is ensured that and it is clean, pollution-free, prevent pest and disease damage, and save soil cuttage in the ground of garden
Manpower spent by the management measures such as required weeding, watering, spraying, has saved great amount of cost, has good using valence
Value and realistic meaning.
Specific embodiment
The following examples can make those skilled in the art that the present invention be more fully understood, but not limit in any way
The present invention.However, those skilled in the art should understand that, various changes can be made to it in the form and details, and
Without departing from claims of the present invention limited range.
In following embodiment, nutrient solution prescription are as follows:
| KNO3 | 950mg/L |
| NH4NO3 | 825mg/L |
| KH2PO4 | 85mg/L |
| CaCl2·2H2O | 220mg/L |
| MgSO4·7H2O | 185mg/L |
| MnSO4·6H2O | 22.3mg/L |
| ZnSO4·7H2O | 8.6mg/L |
| CuSO4·5H2O | 0.025mg/L |
| H3BO3 | 6.2mg/L |
| Na2MoO4·2H2O | 0.25mg/L |
| CoCl2·6H2O | 0.025mg/L |
| Thiamine hydrochloride | 10mg/L |
| Puridoxine hydrochloride | 0.5mg/L |
| Niacin | 0.5mg/L |
| Inositol | 100mg/L |
| Na2Fe-EDTA | 28mg/L |
| IBA | 1.5mg/L |
| IAA | 0.5mg/L |
Embodiment 1:
A kind of method of endangered plants hopea chinensis Water culture breeding, prepares, compounding comprising sticking device, inserts
Fringe acquisition and processing, root-growing agent impregnate, cuttage and insert after manage, primary operational is as follows:
(1) sticking device prepares: selecting length × width × height is respectively 35.2cm × 25cm × 9.5cm plastic casing as training
Container to be supported, uses the pearl cotton plate of 1.5cm thickness as cuttage plate, the specification length and width of cuttage plate are respectively less than culture vessel length and width 5cm,
And cutting hole is beaten with punch, cuttage aperture is 0.5cm, the interval 5cm in every cuttage hole, builds long 200cm with angle steel, wide
5 layers of glass of 100cm are as culturing rack, using 1:24 bromogeramine aqueous solution wiping culture vessel, cuttage plate and culturing rack table
Face is placed on spare in the room equipped with curtain, air-conditioning and humidifier after disinfection；
(2) compounding: nutrient solution is prepared according to formula, after when compounding need individually dissolve each drug
It is mixed again, the hopea chinensis methanol crude extract of 10000mg/L is added, the use of 1mol/L NaOH and salt acid for adjusting pH is 5.8
~6.0, it is stored in 4 DEG C of refrigerators；
(3) cuttings acquires: select robust growth, the select tree without disease pest as maternal plant, acquisition diameter is 0.35~0.45cm,
2 years raw stiff wood items are as cuttings item, and it is 8.5cm that when trimming, which retains cutting length, and the side shoot 0.5cm of bilateral does not retain leaf,
Plane is cut into upper end, and inclined-plane is cut into lower end, and cutting angle and horizontal sextant angle are 60 °, with soft bristle tooth brush light brush cuttings surface when cleaning,
The dish washing liquid aqueous cleaning cuttings surface contaminants for being 0.08% with volume fraction, disinfection impregnate 1min using 75% alcohol, then
With distilled water flushing 3 times, cuttings is obtained；
(4) root-growing agent impregnates: cuttings being soaked in the aqueous solution of 600mg/L GGR, soaking time 2h, soaking depth is
2.5cm；
(5) cuttage: the cuttings oblique cutting that immersion treatment is crossed is entered in the hole accomplished fluently on cuttage plate, is poured into culture vessel
The nutrient solution prepared, nutrient solution highest interface distance culture vessel edge 3cm, cuttage plate is put into nutrient solution, cuttings is inserted
Entering cuttage plate posterior end exposed length is 2cm, and cuttings lower end exposed portion is kept to be completely immersed in nutrient solution.
(6) it is managed after inserting: after inserting the good curtain dark culture 1d of post-tensioning, drawing the curtain apart using nature optical culture, maintain Indoor Temperature
22~26 DEG C of degree, humidity 85~90%, 10d replace the nutrient solution being equally formulated.
Embodiment 2:
A kind of method of endangered plants hopea chinensis Water culture breeding, prepares, compounding comprising sticking device, inserts
Fringe acquisition and processing, root-growing agent impregnate, cuttage and insert after manage, primary operational is as follows:
(1) sticking device prepares: selecting length × width × height is respectively 35.2cm × 25cm × 9.5cm plastic casing as training
Container to be supported, uses the pearl cotton plate of 2.5cm thickness as cuttage plate, the specification length and width of cuttage plate are respectively less than culture vessel length and width 3cm,
And cutting hole is beaten with punch, cuttage aperture is 0.3cm, the interval 3cm of each cutting hole, builds long 180cm with angle steel, wide
5 layers of glass of 80cm are as culturing rack, using 1:24 bromogeramine aqueous solution wiping culture vessel, cuttage plate and culturing rack table
Face is placed on spare in the room equipped with curtain, air-conditioning and humidifier after disinfection；
(2) compounding: nutrient solution is prepared according to formula, after when compounding need individually dissolve each drug
It is mixed again, the hopea chinensis methanol crude extract of 10000mg/L is added, the use of 1mol/L NaOH and salt acid for adjusting pH is 5.8
~6.0, it is stored in 4 DEG C of refrigerators；
(3) cuttings acquires: select robust growth, the select tree without disease pest as maternal plant, acquisition diameter is 0.25~0.38cm,
1 year raw stiff wood item is as cuttings item, and it is 12.5cm that when trimming, which retains cutting length, and the side shoot 0.8cm of bilateral does not retain leaf
Plane is cut into son, upper end, and inclined-plane is cut into lower end, and cutting angle and horizontal sextant angle are 60 °, with the light brush cuttings table of soft bristle tooth brush when cleaning
Face, the dish washing liquid aqueous cleaning cuttings surface contaminants for being 0.08% with volume fraction, disinfection impregnate 3min using 75% alcohol,
It uses distilled water flushing 5 times again, obtains cuttings；
(4) root-growing agent impregnates: cuttings being soaked in the aqueous solution of 800mg/L GGR, soaking time 1h, soaking depth is
4.5cm；
(5) cuttage: the cuttings oblique cutting that immersion treatment is crossed is entered in the hole accomplished fluently on cuttage plate, is poured into culture vessel
The nutrient solution prepared, nutrient solution highest interface distance culture vessel edge 1cm, cuttage plate is put into nutrient solution, cuttings is inserted
Entering cuttage plate posterior end exposed length is 4cm, and cuttings lower end exposed portion is kept to be completely immersed in nutrient solution.
(6) it is managed after inserting: after inserting post-tensioning good curtain darkroom culture 1d, drawing the curtain apart using nature optical culture, remain indoor
20~24 DEG C of temperature, humidity 80~85%, 8d replaces the nutrient solution being equally formulated.
Comparative example 1:
A kind of method of endangered plants hopea chinensis solid mixed-matrix cutting propagation prepares, cuttings comprising sticking device
Acquisition, root-growing agent impregnate, cuttage and insert after manage, primary operational is as follows:
(1) sticking device prepares: using black flexible plastic hole tray as cuttage vessel, volume ratio is the peat soil of 4:2:1: light
Matrix: vermiculite mixed-matrix is packed into diameter 7cm, highly in the transparent plastic bag of 11cm, to be put into 50 hole hole tray of flexible plastic, skewer
Previous daily 0.2~0.5% liquor potassic permanganate is inserted to drench cutting medium 2 times, then and rinsed well with clear water, cuttage is held
Device, which is placed on, builds long 200cm with angle steel, and 5 layers of glass of wide 100cm are wiped as on culturing rack using 1:24 bromogeramine aqueous solution
Culturing rack surface is wiped, is placed on after disinfection spare in the room equipped with curtain, air-conditioning and humidifier；
(2) cuttings acquires: select robust growth, the select tree without disease pest as maternal plant, acquisition diameter is 0.35~0.45cm,
1~2 year raw stiff wood item is as cuttings item, and it is 8.5cm that when trimming, which retains cutting length, and the side shoot 0.5cm of bilateral does not retain leaf
Plane is cut into son, upper end, and inclined-plane is cut into lower end, and cutting angle and horizontal sextant angle are 60 °, with the light brush cuttings table of soft bristle tooth brush when cleaning
Face, the dish washing liquid aqueous cleaning cuttings surface contaminants for being 0.08% with volume fraction, disinfection impregnate 1min using 75% alcohol,
It uses distilled water flushing 3 times again, obtains cuttings；
(3) root-growing agent impregnates: cuttings being soaked in the aqueous solution of 600mg/L GGR, soaking time 2h, soaking depth is
2.5cm；
(4) cuttage: the cuttings that immersion treatment is crossed is inserted perpendicularly into matrix；
(5) it is managed after inserting: after inserting post-tensioning good curtain darkroom culture 1d, drawing the curtain apart using nature optical culture, remain indoor
20~26 DEG C of temperature, humidity 85~90%.
Comparative example 2:
A kind of method of endangered plants hopea chinensis solid mixed-matrix cutting propagation prepares, cuttings comprising sticking device
Acquisition, root-growing agent impregnate, cuttage and insert after manage, primary operational is as follows:
(1) sticking device prepares: using black flexible plastic hole tray as cuttage vessel, volume ratio is the peat soil of 4:2:1: light
Matrix: vermiculite mixed-matrix is packed into diameter 7cm, highly in the transparent plastic bag of 11cm, to be put into 50 hole hole tray of flexible plastic, skewer
Previous daily 0.2~0.5% liquor potassic permanganate is inserted to drench cutting medium 2 times, then and rinsed well with clear water, cuttage is held
Device, which is placed on, builds long 180cm with angle steel, and 5 layers of glass of wide 80cm are wiped as on culturing rack using 1:24 bromogeramine aqueous solution
Culturing rack surface is wiped, is placed on after disinfection spare in the room equipped with curtain, air-conditioning and humidifier；
(2) cuttings acquires: select robust growth, the select tree without disease pest as maternal plant, acquisition diameter is 0.25~0.35cm,
1 year raw stiff wood item is as cuttings item, and it is 12.5cm that when trimming, which retains cutting length, and the side shoot 0.8cm of bilateral does not retain leaf
Plane is cut into son, upper end, and inclined-plane is cut into lower end, and cutting angle and horizontal sextant angle are 60 °, with the light brush cuttings table of soft bristle tooth brush when cleaning
Face, the dish washing liquid aqueous cleaning cuttings surface contaminants for being 0.08% with volume fraction, disinfection impregnate 3min using 75% alcohol,
It uses distilled water flushing 5 times again, obtains cuttings；
(3) root-growing agent impregnates: cuttings being soaked in the aqueous solution of 800mg/L GGR, soaking time 1h, soaking depth is
4.5cm；
(4) cuttage: the cuttings that immersion treatment is crossed is inserted perpendicularly into matrix；
(5) it is managed after inserting: after inserting the good curtain dark culture 1d of post-tensioning, drawing the curtain apart using nature optical culture, maintain Indoor Temperature
20~24 DEG C of degree, humidity 80~85%.
Rooting rate after the earliest rootage duration and 45d of Statistics Implementation example 1-2 and comparative example 1-2, is shown in Table 1.
Table 1: rooting rate after the earliest rootage duration of embodiment and comparative example and 45d
| Group number | Training method | Earliest rootage duration | Rooting rate after 45d |
| Embodiment 1 | Water culture | 10d | 90.2% |
| Embodiment 2 | Water culture | 13d | 91.6% |
| Comparative example 1 | Matrix cuttage | 28d | 73.2% |
| Comparative example 2 | Matrix cuttage | 30d | 70.7% |
From the data in table 1, it can be seen that the method culture hopea chinensis bred using Water culture provided by the present application, when taking root
Between can shift to an earlier date 25d, 45d survival rate can reach 90% or more, can significantly improve its breeding rooting rate and rooting rate,
It is significantly higher than solid mixed-matrix cuttage and seedling culture, substantially reduces the cuttage and seedling culture period.
Claims (9)
1. a kind of method of endangered plants hopea chinensis Water culture breeding, which is characterized in that prepare comprising sticking device, nutrition
Liquid prepare, cuttings acquisition and processing, root-growing agent impregnate, cuttage and insert after manage, key step is as follows:
(1) sticking device prepares: selecting length × width × height is respectively that 35.2cm × 25cm × 9.5cm plastic casing holds as culture
Device uses the pearl cotton plate of 1.5~2.5cm thickness as cuttage plate, is built with angle steel and grow 180~200cm, and the 5 of wide 80~100cm
Layer glass plate is placed on spare in the room equipped with curtain, air-conditioning and humidifier as culturing rack after disinfection；
(2) compounding: nutrient solution is prepared according to formula as below, the hopea chinensis methanol crude extract of 10000mg/L is added, makes
It is 5.8~6.0 with 1mol/L NaOH and salt acid for adjusting pH, is stored in 4 DEG C of refrigerators；
(3) cuttings acquires: it selects robust growth, the select tree without disease pest as maternal plant, acquires stiff wood item as cuttings item, trim,
Cleaning, disinfection obtain cuttings；
(4) root-growing agent impregnates: cuttings being soaked in root-growing agent, soaking depth is 2.5~4.5cm；
(5) cuttage: the cuttings oblique cutting that immersion treatment is crossed is entered in the hole accomplished fluently on cuttage plate, pours into and prepares into culture vessel
Nutrient solution, cuttage plate is put into nutrient solution by nutrient solution highest interface distance culture vessel 1~3cm of edge；
(6) it is managed after inserting: after inserting post-tensioning good curtain darkroom culture 1d, drawing the curtain apart using nature optical culture, maintain room temperature
20~26 DEG C, humidity 80~90%, 7~10d replaces the nutrient solution being equally formulated.
2. the method for hopea chinensis Water culture breeding according to claim 1, it is characterised in that: step (1) described skewer
The specification length and width of plate are respectively less than culture vessel 3~5cm of length and width, and beat cutting hole with punch, and cuttage aperture is 0.3~
0.5cm, 3~5cm of interval of each cutting hole.
3. the method for hopea chinensis Water culture breeding according to claim 1, it is characterised in that: step (1) is described to disappear
Poison is using 1:24 bromogeramine aqueous solution wiping culture vessel, cuttage plate and culturing rack surface.
4. the method for hopea chinensis Water culture breeding according to claim 1, it is characterised in that: step (2) described battalion
Nutrient solution mixes again after need individually dissolving each drug when preparing.
5. the method for hopea chinensis Water culture breeding according to claim 1, it is characterised in that: step (3) is described to insert
The diameter of fringe bar is 0.25~0.45cm, 1~2 year raw stiff wood item.
6. the method for hopea chinensis Water culture breeding according to claim 1, it is characterised in that: step (3) is described to repair
Shear method is to retain cutting length for 8.5~12.5cm, and 0.5~0.8cm of side shoot of bilateral does not retain leaf, and upper end is cut into flat
Inclined-plane is cut into face, lower end, and cutting angle and horizontal sextant angle are 60 °.
7. the method for hopea chinensis Water culture breeding according to claim 1, it is characterised in that: step (3) is described clear
Washing method is with soft bristle tooth brush light brush cuttings surface, the dish washing liquid aqueous cleaning cuttings surface dirt for being 0.08% with volume fraction
Object；The disinfection impregnates 1~3min using 75% alcohol, then with distilled water flushing 3~5 times.
8. the method for hopea chinensis Water culture breeding according to claim 1, it is characterised in that: step (4) described life
The aqueous solution with 600~800mg/L GGR is impregnated in root agent, and soaking time is 1~2h.
9. the method for hopea chinensis Water culture breeding according to claim 1, it is characterised in that: step (5) described skewer
It is 2~4cm that insert method, which is by cuttings insertion cuttage plate posterior end exposed length, and cuttings lower end exposed portion is kept to be completely immersed in battalion
In nutrient solution.
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| CN113207453A (en) * | 2021-06-02 | 2021-08-06 | 云南省林业和草原科学院 | Tea tree cuttage twig long-distance keep-alive method |
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