CN101548687A - Method for extracting sterilization original liquid from broad leaf trees - Google Patents
Method for extracting sterilization original liquid from broad leaf trees Download PDFInfo
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- CN101548687A CN101548687A CNA2009100982659A CN200910098265A CN101548687A CN 101548687 A CN101548687 A CN 101548687A CN A2009100982659 A CNA2009100982659 A CN A2009100982659A CN 200910098265 A CN200910098265 A CN 200910098265A CN 101548687 A CN101548687 A CN 101548687A
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- dried powder
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- zymotic fluid
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Abstract
The invention provides a method for extracting sterilization original liquid from broad leaf trees. The method comprises: (1) taking the plant fragments of a broad leaf tree, crushing, drying into dried powder, adding the dried powder into water and soaking for 5-8 hours, adding magnesium sulfate or potassium sulfate, adding sodium hydroxide or potassium hydroxide to adjust the solution pH value to 8-12, heating to 80-100 degrees, preserving heat for 60-90 min, filtering, performing heat preservation and lixiviation to the filter residue by sodium hydroxide or potassium hydroxide aqueous solution with pH value of 8-12 at 80-100 degrees for 60-90 min, filtering; merging filtrates to obtain first lixiviation liquid; (2) fermenting the first lixiviation liquid at 68-88 degrees until milk yellow or pink crystallization appears in the fermentation liquid, and filtering when the fermentation ends to obtain fermentation liquid; (3) putting the fermentation liquid into a evaporator, holding the temperature at 85-110 degrees and performing steam separation for 8-10 hours, and cooling the residual liquid to obtain the sterilization original liquid. The main beneficial effects of the present invention is: the sterilization original liquid obtained by the method in the invention has strong killing effect to the 'super pathogen', has a wide application prospect.
Description
(1) technical field
The present invention relates to a kind of method of extracting sterilization original liquid from deciduous species, this sterilization original liquid has good killing effect to " superbug ".
(2) background technology
Along with the growing interest of the mankind to self health and environmental consciousness, bacterium is more and more noticeable to the threat of human health." superbug " is called pesticide resistance " staphylococcus aureus " (Staphyloccocus aureus Rosenbach), it is human a kind of important pathogen, be under the jurisdiction of staphylococcus (Staphylococcus), can cause multiple severe infections, the another name of " having a liking for the meat bacterium " is arranged.This courses of infection becomes the problem of many countries headache day by day, wreaks havoc in the U.S. at present, and the trend that surpasses SARS in 2003 is arranged greatly.According to the survey report of in October, 2008 U.S., this germ caused nearly 20,000 people to get killed in 2005, had more threat than acquired immune deficiency syndrome (AIDS).In June, 2008, the statistical data of Reuter's issue showed that the whole world has 50,000,000 people to carry this fatal " superbug " approximately.Only in England and Wales, from 2003 to 2004, the superbug causing death leads just rose 22%.In mid-October, 2008, a Government Report of U.S.'s issue shows that annual have American more than 90,000 to catch possibility fatal " superbug " approximately.
" superbug " spread to all over the world after Britain is found first in 1961 gradually, and initial is infected in hospital's scope, but in recent years, the gesture of relay gradually rises, and has invaded school, the very big place of these density of population of intensive dwelling house.Generally, the people who looks well also may carry this " superbug ", because it can be survived at the skin or the nose of human body, mainly is by the contact transmission between skin, therefore its routes of infection are quite extensive, are present in the middle of the human daily life.
The danger of " superbug " is that it has formed antibody to some common antibiotics, and the antibiotic that can be used for tackling it is fewer and feweri.How to effectively prevent the harm of " superbug ", still be among the arguement.Up to the present, the U.S. does not have any relevant preventive measure to stop spreading of it yet, and medical circle is not developed the vaccine that can effectively tackle " superbug " at present yet.
(3) summary of the invention
The object of the invention provides a kind of extracting method that " superbug " is had the good killing effect sterilization original liquid.
The technical solution used in the present invention is:
A kind of method of from read the leaf seeds, extracting sterilization original liquid, described method comprises:
(1) fetches phytoclasts from broad-leaved tree, be crushed to 30～100 orders, the dry dried powder that gets, with dried powder be added to soak 5～8 hours in the water that quality is 20～35 times of dried powder quality after, adding quality is the magnesium sulfate or the potassium sulphate of dried powder quality 0.02%～10%, and add sodium hydroxide or potassium hydroxide regulator solution pH value is 8～12, be warming up to 80～100 ℃ of insulation 60～90min, filter, filter residue adds the water of 5～10 times of filter residue quality, transfer pH to 8～12 with sodium hydroxide or potassium hydroxide, in 80～100 ℃ of insulation lixiviate 60～90min, filter, merging filtrate obtains extract just;
(2) first extract is in 68～88 ℃ of bottom fermentations, milk yellow appears in zymotic fluid or pink crystallization (needs 10～30 days usually, through repetition test repeatedly, find whether to occur in the zymotic fluid crystallization and extract whether successful important indicator for judging, if milk yellow or pink crystallization do not occur, the then final material that obtains that extracts is the energy that do not have microbe killing properties), turn 2～3 every day between yeast phase, and fermentation ends is filtered and is obtained zymotic fluid;
(3) zymotic fluid places evaporator, keeps temperature to be 85～110 ℃ and carries out steam separation 8～10 hours, and the remaining liq cooling obtains described sterilization original liquid.
Described broad-leaved tree is common broad-leaved tree seeds, can be one of following or wherein two or more mixing: cypress CupressusfunebrisEndl (Cupressaceae), teak Tectona grandis Verbenaceae (Verenaceae), Korean pine Pinus koraiensis Sieb.et Zucc (Pinaceae), white birch Betulaplatyphylla Suk. (Betulaceae), hard maple wood Acer spp.A.saccharum A.nigrumAceraceae (Aceraceae), soft maple wood Acerrubrum.A.saccharum Aceraceae (Aceraceae), Milan Aglaia spp.Mcliaccae (Meliaceae), shield seed wood Bagassa guianensisApocynaceae (Apocynaceae), LIGNUM SAPPAN Balfourdend ron rieddlianan Leguminosae (pulse family) Guntambu Betulaspp.Rutaceae (Rutaceae), light Bao Di beans Brosimumalicastrum Leguminosae (pulse family), green handle mulberry chlorophora spp.Moraceae (Moraceae), China fir Cunningha mia lanceolata Taxodiaceae (Taxodiaceae), twin columns bush Dicoryniaguianensis Leguminosae (pulse family), kapur Dipterocarp usspp Dipterocarpaceae (Dipterocarpaceae), the fragrant Dryobalano ps spp Dipterocarpa ceae (Dipterocarpaceae) of borneol, Entandrophragma cylindricum Sprague Entandr ophragma cylindricum Meliaceae (Meliaceae), red gum Eucalyptus spp Myrtaccae (Myrtaceae), Pontianak iron camphor tree Eusideroxyl on zwageriLauraceae (Lauraceae), beech Fagus spp Fagaceae (the fragrant section of shell), Manchurian ash Fraxinusmandshurica Oleaceae (Oleaceae), appoint loud, high-pitched sound to coat with lacquer wooden Glutaspp.Melanochyl a spp.melanorrhoea Anacardiaceae (Anacardiaceae), light hopea hainanensis Hopea spp Dipterocarpaceae (Dipterocarpaceae), seal eggplant Intsia spp.Leguminosae (pulse family), Ke Ku wood Kokoonaspp.Celastraceae (Wei Yuke), sweet crotons Koompassia spp.Leguminosae (pulse family), sub-capital Madhuca spp Sapotaceae (Sapotaceae), U.S. pigeonpea pericopsis sppLeguminosae (pulse family), red oak Querces spp Fagaceae (Fagaceae), the wooden Rhodoleia spp of red bud Hamamelida ceae (Hamamelidaceae), heavy red Suo Luo Shuan (being beautiful wingceltis) Shoreaspp Dipterocarpa ceae (Dipterocarpaceae), ant wood Tabebuia spp Bignoniaceae (Bignoniaceae), five-leaved chasts tree vitex quinata Verbenaceae (Verenaceae), pyinkado xyliaSpp Leguminosae (pulse family), lignum cinnamomi camphorae Cinnamonum camphora (L.) Presl (Lauraceae), rosewood 0rmosia henryi Prain (pulse family), face cream pigeonpea (being red santal) Myroxylinbalsamum Leguminosae (pulse family), Chamaecyparis formosensis Chamaecyparis formosensis (Cupressaceae).
Described phytoclasts can be preferably root and/or stem from broad-leaved tree from root, stem (comprising bark, trunk), leaf, shell, flower or the material secretion of broad-leaved tree.
Preferably, raw material deciduous species of the present invention are one of following or wherein two or more combinations: cypress CupressusfunebrisEndl, teak Tectona grandis Verbenaceae, lignum cinnamomi camphorae Cinnamonum camphora (L.) Presl, rosewood 0rmosia henryi Prain, heavy red Suo Luo Shuan Shoreaspp Dipterocarpa ceae, kapur Dipterocarp usspp Dipterocarpaceae, face cream pigeonpea Myroxylin balsamum Leguminosae, Chamaecyparis formosensis Chamaecyparisformosensis, Korean pine Pinus koraiensis Sieb.et Zucc, white birch Betula platyphyllaSuk..
Concrete, described method is as follows:
(1) fetches phytoclasts from the broad-leaved tree root, described phytoclasts is from cypress, teak, lignum cinnamomi camphorae, rosewood, heavy red Suo Luo Shuan, kapur, the phytoclasts of face cream pigeonpea root was with mass ratio 3: 2: 2: 1: 1: 0.5: 0.5 mixture, phytoclasts is crushed to 30 orders, dry dried powder, with dried powder be added to soak 5～8 hours in the water that quality is 25～30 times of dried powder quality after, adding quality is the magnesium sulfate or the potassium sulphate of dried powder quality 0.02%～10%, and add sodium hydroxide or potassium hydroxide regulator solution pH value is 8～10, be warming up to 90～100 ℃ of insulation 60～80min, filter, the filter residue water of 5～10 times of filter residue quality, transfer pH to 8～10 with sodium hydroxide or potassium hydroxide, in 90～100 ℃ of insulation lixiviate 60～80min, filter, merging filtrate obtains extract just;
(2) extract is in 68～88 ℃ of bottom fermentations, occurs in zymotic fluid that turn 2～3 every day between milk yellow or pink crystallization yeast phase, and fermentation ends is filtered and obtained zymotic fluid;
(3) zymotic fluid places evaporator, keeps temperature to be 85～110 ℃ and carries out steam separation 8～10 hours, and the remaining liq cooling promptly obtains described sterilization original liquid.
Perhaps, described method is as follows:
(1) fetches phytoclasts from the broad-leaved tree root, described phytoclasts is from cypress, teak, the phytoclasts of lignum cinnamomi camphorae root is with 1: 1: 3 mixture of mass ratio, phytoclasts is crushed to 30 orders, dry dried powder, with dried powder be added to soak 5～8 hours in the water that quality is 25～30 times of dried powder quality after, adding quality is the magnesium sulfate or the potassium sulphate of dried powder quality 0.02%～10%, and add sodium hydroxide or potassium hydroxide regulator solution pH value is 8～10, be warming up to 90～100 ℃ of insulation 60～80min, filter, filter residue adds the water of 5～10 times of filter residue matter, transfer pH to 8～10 with sodium hydroxide or potassium hydroxide, in 90～100 ℃ of insulation lixiviate 60～80min, filter, merging filtrate obtains extract just;
(2) just extract milk yellow or pink crystallization occur in 68～88 ℃ of bottom fermentations in zymotic fluid, and turn 2～3 every day between yeast phase, and fermentation ends is filtered and obtained zymotic fluid;
(3) zymotic fluid places evaporator, keeps temperature to be 85～110 ℃ and carries out steam separation 8～10 hours, and the remaining liq cooling obtains described sterilization original liquid.
Broad-leaved tree sterilization original liquid of the present invention extracts from forest plants, preparation process can not cause environmental pollution, safety non-toxic, detect proof, this extract can be eliminated " superbug ", because " superbug " has no resistance to it, so this extract is the jinx of " superbug ".This sterilization original liquid can be prepared into the natural antibacterial dye liquor and be used for textile dyeing, and the part fabric is not only nontoxic after dyeing through the natural antibacterial dye for preparing, and has antibacterial functions.And advocate under nontoxic, as to have the health care consumer goods tide impact current people, it will have vast potential for future development.
Beneficial effect of the present invention is mainly reflected in: extract the sterilization original liquid that obtains according to the inventive method " superbug " had strong killing action, at clothes, daily use chemicals, field of medicaments great role is arranged, have a extensive future.
(4) description of drawings
Fig. 1 is the used evaporator figure of the embodiment of the invention.
(5) embodiment
The present invention is described further below in conjunction with specific embodiment, but protection scope of the present invention is not limited in this:
Embodiment 1: sterilization original liquid extracts
1, get cypress, teak, lignum cinnamomi camphorae, rosewood, heavy red Suo Luo Shuan, kapur, the phytoclasts of face cream pigeonpea root was with mass ratio 3: 2: 2: 1: 1: 0.5: 0.5 mixture, phytoclasts is crushed to 30 orders, dry dried powder, after soaking 8 hours in the water with dried powder 2kg adding 50kg, the magnesium sulfate that adds quality 0.02kg, and adding sodium hydrate regulator solution pH value is 9, be warming up to 90～100 ℃ of insulation 80min, filter, filter residue adds the water of 8 times of filter residue quality, transfer pH to 9 with sodium hydroxide, in 90～100 ℃ of insulation lixiviate 80min, filter, merging filtrate obtains extract just;
2, first extract is in 80 ℃ of bottom fermentations, and turn 2 every day between yeast phase, occurs pink crystallization in the time of about 30 days in the zymotic fluid, stops fermentation, filters and obtains zymotic fluid;
3, zymotic fluid places evaporator shown in Figure 1, open the preheater of evaporator, open the steam valve of evaporator top simultaneously, closing liquid outlet valve, keep temperature to be 90～100 ℃ and carry out the steam separation after 10 hours, open the liquid outlet valve of base of evaporator, begin to cool down, cooling finishes and obtains described sterilization original liquid.
Embodiment 2:
1, the phytoclasts of getting rosewood, kapur, face cream pigeonpea, Chamaecyparis formosensis stem was with mass ratio 3: 3: 2: 2 mixture, with phytoclasts be crushed to 30 orders, dry dried powder, after soaking 6 hours in the water with dried powder 2kg adding 40kg, the potassium sulphate that adds 0.1kg, and adding sodium hydrate regulator solution pH value is 10, be warming up to 90～100 ℃ of insulation 60min, filter, filter residue is with the water of filter residue 6 times of quality, transfer pH to 10 with potassium hydroxide, in 90～100 ℃ of insulation lixiviate 60min, filter, merging filtrate obtains extract just;
2, first extract is in 85 ℃ of bottom fermentations, and turn 2 every day between yeast phase, the milk yellow crystallization occurs in the zymotic fluid in the time of about 20 days, stops fermentation, filters and obtains zymotic fluid;
3, zymotic fluid places evaporator, open branch road pump, preheating and the heater of evaporator, open the valve of vapour separator simultaneously, keep temperature to be 100～110 ℃ and carry out the steam separation after 8 hours, open the liquid outlet valve of base of evaporator, begin to cool down, cooling finishes and obtains described sterilization original liquid.
Embodiment 3:
1, gets the phytoclasts of cypress, teak, lignum cinnamomi camphorae root with 1: 1: 3 mixture of mass ratio, with phytoclasts be crushed to 30 orders, dry dried powder, after soaking 8 hours in the water with dried powder 1.5kg adding 45kg, the potassium sulphate that adds 0.03kg, and adding potassium hydroxide regulator solution pH value is 8, be warming up to 90～100 ℃ of insulation 80min, filter, filter residue is with the water of 10 times of filter residue quality, transfer pH to 8 with potassium hydroxide, in 90～100 ℃ of insulation lixiviate 80min, filter, merging filtrate obtains extract just;
2, first extract is in 75 ℃ of bottom fermentations, and turn 2 every day between yeast phase, the milk yellow crystallization occurs in the time of about 30 days, stops fermentation, and fermentation ends is filtered and obtained zymotic fluid;
3, zymotic fluid places evaporator, open the preheater of evaporator, open the steam valve of evaporator top simultaneously, closing liquid outlet valve, keep temperature to be 85～98 ℃ and carry out the steam separation after 10 hours, open the liquid outlet valve of base of evaporator, begin to cool down, cooling finishes and obtains described sterilization original liquid.
Embodiment 4:
1, gets the mixture of the phytoclasts of cypress root and rosewood stem with mass ratio mixing in 4: 1, with phytoclasts be crushed to 30 orders, dry dried powder, after soaking 8 hours in the water with dried powder 2kg adding 55kg, the potassium sulphate that adds 0.08kg, and adding sodium hydrate regulator solution pH value is 9, be warming up to 90～100 ℃ of insulation 80min, filter, filter residue is with the water of filter residue quality 10, with sodium hydroxide accent pH to 9, in 90～100 ℃ of insulation lixiviate 80min, filter, merging filtrate obtains extract just;
2, first extract is in 85 ℃ of bottom fermentations, and turn 2 every day between yeast phase, the milk yellow crystallization occurs in the time of about 20 days, stops fermentation, filters and obtains zymotic fluid;
3, zymotic fluid places evaporator, open the preheater of evaporator, open the steam valve of evaporator top simultaneously, closing liquid outlet valve, keep temperature to be 90～100 ℃ and carry out the steam separation after 10 hours, open the liquid outlet valve of base of evaporator, begin to cool down, cooling finishes and obtains described sterilization original liquid.
Embodiment 6:
1, get hard maple wood, soft maple wood, Milan, shield seed wood, Guntambu, the phytoclasts of balsam wood beanstalk portion was with mass ratio 3: 3: 1: 1: 1: 1 mixture, phytoclasts is crushed to 30 orders, dry dried powder, after soaking 6 hours in the water with dried powder 2kg adding 40kg, the potassium sulphate that adds 0.15kg, and adding sodium hydrate regulator solution pH value is 10, be warming up to 90～100 ℃ of insulation 80min, filter, it is the water of 6 times of filter residue quality that filter residue adds quality, transfer pH to 10 with potassium hydroxide, in 90～100 ℃ of insulation lixiviate 80min, filter, merging filtrate obtains extract just;
2, first extract is in 85 ℃ of bottom fermentations, and turn 2 every day between yeast phase, the milk yellow crystallization occurs in the zymotic fluid in the time of about 21 days, stops fermentation, filters and obtains zymotic fluid;
3, zymotic fluid places evaporator, open the preheater of evaporator, open the steam valve of evaporator top simultaneously, closing liquid outlet valve, keep temperature to be 100～110 ℃ and carry out the steam separation after 8 hours, open the liquid outlet valve of base of evaporator, begin to cool down, cooling finishes and obtains described sterilization original liquid.
Embodiment 7:
1, get LIGNUM SAPPAN, China fir, kapur, Korean pine, white birch, red oak, the phytoclasts of ant wood root was with mass ratio 3: 2: 2: 1: 1: 0.5: 0.5 mixture, phytoclasts is crushed to 30 orders, dry dried powder, after soaking 8 hours in the water with dried powder 2kg adding 50kg, the magnesium sulfate that adds 0.05kg, and adding sodium hydrate regulator solution pH value is 9, be warming up to 90～100 ℃ of insulation 80min, filter, filter residue adds the water of 8 times of filter residue quality, transfer pH to 9 with sodium hydroxide, in 90～100 ℃ of insulation lixiviate 80min, filter, merging filtrate obtains extract just;
2, first extract is in 80 ℃ of bottom fermentations, and turn 2 every day between yeast phase, occurs pink crystallization in the time of about 32 days in the zymotic fluid, stops fermentation, filters and obtains zymotic fluid;
3, zymotic fluid places evaporator, open the preheater of evaporator, open the steam valve of evaporator top simultaneously, closing liquid outlet valve, keep temperature to be 90～100 ℃ and carry out the steam separation after 10 hours, open the liquid outlet valve of base of evaporator, begin to cool down, cooling finishes and obtains described sterilization original liquid.
Embodiment 8:
1, gets the phytoclasts of China fir, borneol perfume (or spice), red gum root with 1: 1: 3 mixture of mass ratio, with phytoclasts be crushed to 30 orders, dry dried powder, after soaking 8 hours in the water with dried powder 1.5kg adding 45kg, the potassium sulphate that adds 0.06kg, and adding potassium hydroxide regulator solution pH value is 8, be warming up to 90～100 ℃ of insulation 80min, filter, filter residue is with the water of filter residue 10 times of quality, transfer pH to 8 with potassium hydroxide, in 90～100 ℃ of insulation lixiviate 80min, filter, merging filtrate obtains extract just;
2, first extract is in 75 ℃ of bottom fermentations, and turn 2 every day between yeast phase, the milk yellow crystallization occurs in the time of about 28 days, stops fermentation, and fermentation ends is filtered and obtained zymotic fluid;
3, zymotic fluid places evaporator, open the preheater of evaporator, open the steam valve of evaporator top simultaneously, closing liquid outlet valve, keep temperature to be 85～98 ℃ and carry out the steam separation after 10 hours, open the liquid outlet valve of base of evaporator, begin to cool down, cooling finishes and obtains described sterilization original liquid.
Embodiment 9:
1, gets the mixture of the phytoclasts of green handle mulberry root and twin columns bush stem with mass ratio mixing in 4: 1, with phytoclasts be crushed to 30 orders, dry dried powder, after soaking 8 hours in the water with dried powder 2kg adding 55kg, the magnesium sulfate that adds 0.08kg, and adding sodium hydrate regulator solution pH value is 9, be warming up to 90～100 ℃ of insulation 80min, filter, filter residue add 10 times of quality of filter residue water, transfer pH to 9 with sodium hydroxide, in 90～100 ℃ of insulation lixiviate 80min, filter, merging filtrate obtains extract just;
2, first extract is in 85 ℃ of bottom fermentations, and turn 2 every day between yeast phase, the milk yellow crystallization occurs in the time of about 25 days, stops fermentation, filters and obtains zymotic fluid;
3, zymotic fluid places evaporator, open the preheater of evaporator, open the steam valve of evaporator top simultaneously, closing liquid outlet valve, keep temperature to be 90～100 ℃ and carry out the steam separation after 10 hours, open the liquid outlet valve of base of evaporator, begin to cool down, cooling finishes and obtains described sterilization original liquid.
Embodiment 10～15:
Get cypress, teak, lignum cinnamomi camphorae, rosewood, kapur, Chamaecyparis formosensis root phytoclasts respectively, be crushed to 30 orders, dry dried powder, respectively get the 2kg dried powder and extract sterilization original liquid according to embodiment 1 method, the gained sterilization original liquid carries out the bactericidal effect experiment.
Embodiment 11: the bactericidal effect experiment
(1) test item and test basis:
" disinfection technology standard " version 2.1.1.7 in 2002 bacteria quantified is killed experiment
(2) test strain:
Staphylococcus aureus: ATCC No.6538 (gram-positive bacteria)
(3) method step:
1, get embodiment 1～15 sterilization original liquid, be mixed with the 250mg/L thimerosal with sterile water respectively, it is standby to put 20 ℃ ± 1 ℃ water-bath;
2, the staphylococcus aureus compound concentration 5 * 10
3The bacteria suspension of cfu/mL;
3, the aseptic Boiling tube of cancellation poison test adds the 0.5mL bacteria suspension earlier, adds 0.5mL bovine serum albumin (0.5mg/mL) again, mixing, insert in 20 ℃ ± 1 ℃ water-bath behind the 5min, draw each 4.0mL of above-mentioned thimerosal with aseptic straw and inject wherein, mixing and timing immediately rapidly;
4, treat that experimental bacteria and thimerosal interacted to the scheduled time, draw the mixed liquor 0.5mL of experimental bacteria and thimerosal respectively, be added to 4.5mL in the inorganic agent (sulfuric acid phenol sodium and soybean lecithin mixture, sulfur acid phenol sodium 0.5g) of sterilization, mixing;
5, respectively manage treated dose of mixed liquor effect 10min after, draw 1.0mL sample liquid respectively, measure by the bacterial culture method of counting and deposit viable count, 2 plates of every pipe sample liquid inoculation.As the bacterium number of growing on the flat board more for a long time, can carry out 10 times of dilutions after, carry out viable bacteria again and cultivate counting.
6, replace thimerosal with sterile water simultaneously, carry out parallel test according to step 3～5 operations, as positive control;
7, all test samples are all cultivated in 37 ℃ of incubators, and test repeats 3～4 times, calculate the viable bacteria concentration (cfu/mL) of each pipe, and calculate sterilizing rate with reference to the viable bacteria concentration of positive control.
(4) assay
See Table 1.
Table 1
By table 1 as seen, the bacteriostasis rate of deciduous species extract all reaches more than 96%, this shows that the sterilization original liquid that extracts with broad-leaved tree has the good restraining effect to staphylococcus aureus (" superbug "), and it is nontoxic to human body, can be applicable to clothes, daily use chemicals, medicine and other fields, have broad prospect of application.
Claims (5)
1. method of from deciduous species, extracting sterilization original liquid, described method comprises:
(1) fetches phytoclasts from broad-leaved tree, be crushed to 30～100 orders, the dry dried powder that gets, with dried powder be added to soak 5～8 hours in the water that quality is 20～35 times of dried powder quality after, adding quality is the magnesium sulfate or the potassium sulphate of dried powder quality 0.02%～10%, and add sodium hydroxide or potassium hydroxide regulator solution pH value is 8～12, be warming up to 80～100 ℃ of insulation 60～90min, filter, filter residue adds the water of 5～10 times of filter residue quality, sodium hydroxide or potassium hydroxide are transferred pH to 8～12, in 80～100 ℃ of insulation lixiviate 60～90min, filter, merging filtrate obtains extract just;
(2) just extract milk yellow or pink crystallization occur in 68～88 ℃ of bottom fermentations in zymotic fluid, and turn 2～3 every day between yeast phase, and fermentation ends is filtered and obtained zymotic fluid;
(3) zymotic fluid places evaporator, keeps temperature to be 85～110 ℃ and carries out steam separation 8～10 hours, and the remaining liq cooling is described sterilization original liquid;
Described broad-leaved tree is one of following or wherein two or more mixing: cypress CupressusfunebrisEndl, teak Tectona grandis Verbenaceae, Korean pine Pinuskoraiensis Sieb.et Zucc, white birch Betula platyphylla Suk., hard maple wood Acer spp.A.saccharum A.nigrum Aceraceae, soft maple wood Acerrubrum.A.saccharumAceraceae, Milan Aglaia spp.Mcliaccae, shield seed wood Bagassa guianensisApocynaceae, LIGNUM SAPPAN Balfourdend ron rieddlianan Leguminosae, Guntambu Betulaspp.Rutaceae, light Bao Di beans Brosimum alicastrum Leguminosae, green handle mulberry chlorophora spp.Moraceae, China fir Cunningha mia lanceolataTaxodiaceae, twin columns bush Dicorynia guianensis Leguminosae, kapur Dipterocarp usspp Dipterocarpa ceae, the fragrant Dryobalano ps of borneol sppDipterocarpa ceae, Entandrophragma cylindricum Sprague Entandr ophragma cylindricum Meliaceae, red gum Eucalyptus spp Myrtaccae, Pontianak iron camphor tree Eusideroxyl on zwageriLauraceae, beech Fagus spp Fagaceae, Manchurian ash Fraxinus mandshuricaOleaceae, appoint loud, high-pitched sound to coat with lacquer wooden Glutaspp.Melanochyl a spp.melanorrhoeaAnacardiaceae, light hopea hainanensis Hopea spp Dipterocarpa ceae, seal eggplant Intsia spp.Leguminosae, Ke Ku wood Kokoona spp.Celastraceae, sweet crotons Koompassia spp.Leguminosae, sub-capital Madhuca spp Sapotaceae, U.S. pigeonpea pericopsis sppLeguminosae, red oak Querces spp Fagaceae, the wooden Rhodoleia sppHamamelida of red bud ceae, heavy red Suo Luo Shuan Shoreaspp Dipterocarpa ceae, ant wood Tabebuia spp Bignoniaceae, five-leaved chasts tree vitex quinata Verbenaceae, pyinkado xylia Spp Leguminosae, lignum cinnamomi camphorae Cinnamonum camphora (L.) Presl, rosewood 0rmosia henryi Prain, face cream pigeonpea Myroxylin balsamum Leguminosae, Chamaecyparis formosensis Chamaecyparis formosensis.
2. the method for claim 1 is characterized in that root and/or the stem of described phytoclasts from broad-leaved tree.
3. the method for claim 1 is characterized in that described broad-leaved tree is one of following or wherein two or more combination: cypress CupressusfunebrisEndl, teak Tectona grandisVerbenaceae, lignum cinnamomi camphorae Cinnamonum camphora (L.) Presl, rosewood 0rmosiahenryi Prain, heavy red Suo Luo Shuan Shoreaspp Dipterocarpa ceae, kapur Dipterocarp usspp Dipterocarpa ceae, face cream pigeonpea Myroxylin balsamumLeguminosae, Chamaecyparis formosensis Chamaecyparis formosensis, Korean pine Pinus koraiensisSieb.et Zucc, white birch Betula platyphylla Suk..
4. the method for claim 1 is characterized in that described method is as follows:
(1) fetches phytoclasts from the broad-leaved tree root, described phytoclasts is from cypress, teak, lignum cinnamomi camphorae, rosewood, heavy red Suo Luo Shuan, kapur, the phytoclasts of face cream pigeonpea root was with mass ratio 3: 2: 2: 1: 1: 0.5: 0.5 mixture, phytoclasts is crushed to 30 orders, dry dried powder, with dried powder be added to soak 5～8 hours in the water that quality is 25～30 times of dried powder quality after, adding quality is the magnesium sulfate or the potassium sulphate of dried powder quality 0.02%～10%, and add sodium hydroxide or potassium hydroxide regulator solution pH value is 8～10, be warming up to 90～100 ℃ of insulation 60～80min, filter, filter residue adds the water of 5～10 times of filter residue quality, transfer pH to 8～10 with sodium hydroxide or potassium hydroxide, in 90～100 ℃ of insulation lixiviate 60～80min, filter, merging filtrate obtains extract just;
(2) just extract is in 68～88 ℃ of bottom fermentations, and turn 2～3 every day between yeast phase, occurs milk yellow or pink crystallization in zymotic fluid, and fermentation ends is filtered and obtained zymotic fluid;
(3) zymotic fluid places evaporator, keeps temperature to be 85～110 ℃ and carries out steam separation 8～10 hours, and the remaining liq cooling promptly obtains described sterilization original liquid.
5. the method for claim 1 is characterized in that described method is as follows:
(1) fetches phytoclasts from the broad-leaved tree root, described phytoclasts is from cypress, teak, the phytoclasts of lignum cinnamomi camphorae root is with 1: 1: 3 mixture of mass ratio, phytoclasts is crushed to 30 orders, dry dried powder, with dried powder be added to soak 5～8 hours in the water that quality is 25～30 times of dried powder quality after, adding quality is the magnesium sulfate or the potassium sulphate of dried powder quality 0.02%～10%, and add sodium hydroxide or potassium hydroxide regulator solution pH value is 8～10, be warming up to 90～100 ℃ of insulation 60～80min, filter, filter residue adds the water of 5～10 times of filter residue quality, transfer pH to 8～10 with sodium hydroxide or potassium hydroxide, in 90～100 ℃ of insulation lixiviate 60～80min, filter, merging filtrate obtains extract just;
(2) just extract milk yellow or pink crystallization occur in 68～88 ℃ of bottom fermentations in zymotic fluid, and turn 2～3 every day between yeast phase, and fermentation ends is filtered and obtained zymotic fluid;
(3) zymotic fluid places evaporator, keeps temperature to be 85～110 ℃ and carries out steam separation 8～10 hours, and the remaining liq cooling promptly obtains described sterilization original liquid.
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