CN110177881B - 一种酶法制备瑞鲍迪甙j的方法 - Google Patents

一种酶法制备瑞鲍迪甙j的方法 Download PDF

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Publication number
CN110177881B
CN110177881B CN201680089970.0A CN201680089970A CN110177881B CN 110177881 B CN110177881 B CN 110177881B CN 201680089970 A CN201680089970 A CN 201680089970A CN 110177881 B CN110177881 B CN 110177881B
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rebaudioside
udp
ugt
glycosyltransferase
reaction
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Chinese (zh)
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CN110177881A (zh
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陶军华
李国庆
王文霞
郑雷雷
朱春磊
梁晓亮
陈车翘
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Pepsico Inc
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Pepsico Inc
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L27/00—Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/30—Artificial sweetening agents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/18—Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides
    • C12P19/56—Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01017—Glucuronosyltransferase (2.4.1.17)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/185—Escherichia
    • C12R2001/19—Escherichia coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C12R2001/84—Pichia
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C12R2001/85—Saccharomyces
    • C12R2001/865—Saccharomyces cerevisiae
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/55—Design of synthesis routes, e.g. reducing the use of auxiliary or protecting groups

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Polymers & Plastics (AREA)
  • Food Science & Technology (AREA)
  • Nutrition Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CN201680089970.0A 2016-10-21 2016-10-21 一种酶法制备瑞鲍迪甙j的方法 Active CN110177881B (zh)

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CN202310493375.5A CN116574775A (zh) 2016-10-21 2016-10-21 一种酶法制备瑞鲍迪甙j的方法

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PCT/CN2016/102942 WO2018072211A1 (zh) 2016-10-21 2016-10-21 一种酶法制备瑞鲍迪甙j的方法

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CN110177881B true CN110177881B (zh) 2023-06-02

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US (3) US11359222B2 (enExample)
EP (1) EP3543346A4 (enExample)
JP (1) JP6842800B2 (enExample)
CN (2) CN110177881B (enExample)
AU (1) AU2016427128C1 (enExample)
MX (1) MX2019004630A (enExample)
RU (1) RU2736352C1 (enExample)
WO (1) WO2018072211A1 (enExample)

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* Cited by examiner, † Cited by third party
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CA3041150A1 (en) 2016-10-21 2018-04-26 Pepsico, Inc. Method for preparing rebaudioside c using enzymatic method
CA3041152A1 (en) 2016-10-21 2018-04-26 Pepsico, Inc. Method for preparing rebaudioside n using enzymatic method
CN111868252B (zh) 2018-03-12 2025-06-03 科纳根公司 甜菊糖苷莱苞迪苷j和莱苞迪苷n的生物合成生产

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CN103732753A (zh) * 2011-08-08 2014-04-16 埃沃尔瓦公司 甜菊醇糖苷类的重组生产
CN103757074A (zh) * 2014-01-16 2014-04-30 苏州汉酶生物技术有限公司 一种酶法制备瑞鲍迪甙m的方法
CN103974628A (zh) * 2012-05-22 2014-08-06 谱赛科有限责任公司 高纯度的甜菊醇糖苷
US20150315623A1 (en) * 2014-05-05 2015-11-05 Conagen Inc. Non-caloric sweetener
CN105200098A (zh) * 2015-06-30 2015-12-30 苏州汉酶生物技术有限公司 一种利用酿酒酵母酶法制备瑞鲍迪甙m的方法

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KR102061165B1 (ko) 2008-10-03 2019-12-31 모리타 가가쿠 고교 가부시키가이샤 신규 스테비올 배당체
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Publication number Publication date
BR112019007882A2 (pt) 2019-07-02
EP3543346A4 (en) 2020-07-22
AU2016427128C1 (en) 2023-01-05
CN116574775A (zh) 2023-08-11
US20240287567A1 (en) 2024-08-29
AU2016427128A1 (en) 2019-05-30
MX2019004630A (es) 2019-07-15
US20190338332A1 (en) 2019-11-07
EP3543346A1 (en) 2019-09-25
US11359222B2 (en) 2022-06-14
WO2018072211A1 (zh) 2018-04-26
RU2736352C1 (ru) 2020-11-16
JP2019536444A (ja) 2019-12-19
US20230022453A1 (en) 2023-01-26
CN110177881A (zh) 2019-08-27
JP6842800B2 (ja) 2021-03-17
CA3041147A1 (en) 2018-04-26
AU2016427128B2 (en) 2022-08-18
US11952604B2 (en) 2024-04-09

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