AU2016427128C1 - Method for preparing rebaudioside J using enzymatic method - Google Patents

Method for preparing rebaudioside J using enzymatic method Download PDF

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Publication number
AU2016427128C1
AU2016427128C1 AU2016427128A AU2016427128A AU2016427128C1 AU 2016427128 C1 AU2016427128 C1 AU 2016427128C1 AU 2016427128 A AU2016427128 A AU 2016427128A AU 2016427128 A AU2016427128 A AU 2016427128A AU 2016427128 C1 AU2016427128 C1 AU 2016427128C1
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rebaudioside
ala
leu
udp
ugt
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AU2016427128B2 (en
AU2016427128A1 (en
Inventor
Kuikiu CHAN
Guoqing Li
Xiaoliang LIANG
Alex TAO
Wenxia WANG
Leilei ZHENG
Chunlei Zhu
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Pepsico Inc
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Pepsico Inc
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Assigned to PEPSICO, INC. reassignment PEPSICO, INC. Amend patent request/document other than specification (104) Assignors: PEPSICO INC.
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides
    • C12P19/56—Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L27/00—Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/30—Artificial sweetening agents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/18—Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01017—Glucuronosyltransferase (2.4.1.17)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/185—Escherichia
    • C12R2001/19—Escherichia coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C12R2001/84—Pichia
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C12R2001/85—Saccharomyces
    • C12R2001/865—Saccharomyces cerevisiae
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/55—Design of synthesis routes, e.g. reducing the use of auxiliary or protecting groups

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Nutrition Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
AU2016427128A 2016-10-21 2016-10-21 Method for preparing rebaudioside J using enzymatic method Active AU2016427128C1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/CN2016/102942 WO2018072211A1 (zh) 2016-10-21 2016-10-21 一种酶法制备瑞鲍迪甙j的方法

Publications (3)

Publication Number Publication Date
AU2016427128A1 AU2016427128A1 (en) 2019-05-30
AU2016427128B2 AU2016427128B2 (en) 2022-08-18
AU2016427128C1 true AU2016427128C1 (en) 2023-01-05

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
AU2016427128A Active AU2016427128C1 (en) 2016-10-21 2016-10-21 Method for preparing rebaudioside J using enzymatic method

Country Status (9)

Country Link
US (3) US11359222B2 (enExample)
EP (1) EP3543346A4 (enExample)
JP (1) JP6842800B2 (enExample)
CN (2) CN110177881B (enExample)
AU (1) AU2016427128C1 (enExample)
CA (1) CA3041147A1 (enExample)
MX (1) MX2019004630A (enExample)
RU (1) RU2736352C1 (enExample)
WO (1) WO2018072211A1 (enExample)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2016427130B2 (en) 2016-10-21 2022-08-18 Pepsico, Inc. Method for preparing rebaudioside N using enzymatic method
EP3530746A4 (en) 2016-10-21 2020-06-24 Pepsico, Inc. METHOD FOR PRODUCING REBAUDIOSIDE C BY ENZYMATIC METHOD
CN111868252B (zh) 2018-03-12 2025-06-03 科纳根公司 甜菊糖苷莱苞迪苷j和莱苞迪苷n的生物合成生产

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103757074A (zh) * 2014-01-16 2014-04-30 苏州汉酶生物技术有限公司 一种酶法制备瑞鲍迪甙m的方法

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CN101225424B (zh) 2007-09-13 2013-05-29 天津药物研究院 环黄芪醇的单葡萄糖苷、其制备方法、药物组合物和应用
PL2350110T3 (pl) 2008-10-03 2016-12-30 Nowe glikozydy stewiolowe
ES2661539T3 (es) * 2009-10-15 2018-04-02 Purecircle Sdn Bhd Rebaudiósido D de pureza elevada y aplicaciones
BR122021005297B1 (pt) 2010-06-02 2022-02-22 Evolva, Inc Método para transferir uma segunda porção de açúcar para uma posição c-2' de uma glicose em um glicosídeo de esteviol
US8962698B2 (en) 2011-01-28 2015-02-24 Tate & Lyle Ingredients Americas Llc Rebaudioside-mogroside V blends
JP6262653B2 (ja) * 2011-08-08 2018-01-17 エヴォルヴァ エスアー．Ｅｖｏｌｖａ Ｓａ． ステビオール配糖体の組換え生産
CN103031283B (zh) 2011-10-08 2015-07-08 成都华高瑞甜科技有限公司 甜叶菊酶vi及莱鲍迪苷a转化为莱鲍迪苷d的方法
CN103159808B (zh) 2011-12-09 2017-03-29 上海泓博智源医药股份有限公司 一种制备天然甜味剂的工艺方法
EP3735841A1 (en) 2011-12-19 2020-11-11 PureCircle SDN BHD Methods for purifying steviol glycosides and uses of the same
EA201400836A8 (ru) 2012-01-23 2016-03-31 ДСМ АйПи АССЕТС Б.В. Получение дитерпена
EP2852296B1 (en) * 2012-05-22 2021-12-15 PureCircle SDN BHD Process for producing a high-purity steviol glycoside
US9752174B2 (en) 2013-05-28 2017-09-05 Purecircle Sdn Bhd High-purity steviol glycosides
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CN103088041B (zh) 2013-01-29 2015-01-07 江南大学 一种可用于高效生产角质酶的角质酶基因及其应用
KR20150115002A (ko) 2013-02-06 2015-10-13 에볼바 에스아 레바우디오시드 ｄ 및 레바우디오시드 ｍ의 개선된 생산 방법
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US9522929B2 (en) 2014-05-05 2016-12-20 Conagen Inc. Non-caloric sweetener
EP3183349B1 (en) 2014-08-19 2020-03-25 Purecircle SDN BHD Method for preparing rebaudioside i
AU2015327940B2 (en) * 2014-10-03 2020-04-16 Conagen Inc. Non-caloric sweeteners and methods for synthesizing
CA2987587C (en) 2015-05-29 2022-05-03 Cargill, Incorporated Heat treatment to produce glycosides
CN105200098A (zh) * 2015-06-30 2015-12-30 苏州汉酶生物技术有限公司 一种利用酿酒酵母酶法制备瑞鲍迪甙m的方法
WO2017031424A1 (en) 2015-08-20 2017-02-23 Pepsico, Inc. Preparation of rebaudioside m in a single reaction vessel
AU2016427130B2 (en) 2016-10-21 2022-08-18 Pepsico, Inc. Method for preparing rebaudioside N using enzymatic method
EP3530746A4 (en) 2016-10-21 2020-06-24 Pepsico, Inc. METHOD FOR PRODUCING REBAUDIOSIDE C BY ENZYMATIC METHOD

Patent Citations (1)

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Also Published As

Publication number Publication date
JP6842800B2 (ja) 2021-03-17
EP3543346A4 (en) 2020-07-22
MX2019004630A (es) 2019-07-15
US20240287567A1 (en) 2024-08-29
US11359222B2 (en) 2022-06-14
US11952604B2 (en) 2024-04-09
AU2016427128B2 (en) 2022-08-18
CN110177881A (zh) 2019-08-27
EP3543346A1 (en) 2019-09-25
RU2736352C1 (ru) 2020-11-16
BR112019007882A2 (pt) 2019-07-02
WO2018072211A1 (zh) 2018-04-26
US20230022453A1 (en) 2023-01-26
CA3041147A1 (en) 2018-04-26
AU2016427128A1 (en) 2019-05-30
CN110177881B (zh) 2023-06-02
JP2019536444A (ja) 2019-12-19
US20190338332A1 (en) 2019-11-07
CN116574775A (zh) 2023-08-11

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DA2 Applications for amendment section 104

Free format text: THE NATURE OF THE AMENDMENT IS AS SHOWN IN THE STATEMENT(S) FILED 19 SEP 2022

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Owner name: PEPSICO, INC.

Free format text: FORMER NAME(S): PEPSICO INC.

DA3 Amendments made section 104

Free format text: THE NATURE OF THE AMENDMENT IS AS SHOWN IN THE STATEMENT(S) FILED 19 SEP 2022

FGA Letters patent sealed or granted (standard patent)