CN110114654B - 使用干涉条纹增强对比度并发现焦点的透射照明成像 - Google Patents

使用干涉条纹增强对比度并发现焦点的透射照明成像 Download PDF

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CN110114654B
CN110114654B CN201780080905.6A CN201780080905A CN110114654B CN 110114654 B CN110114654 B CN 110114654B CN 201780080905 A CN201780080905 A CN 201780080905A CN 110114654 B CN110114654 B CN 110114654B
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CN110114654A (zh
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马修·陈
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Molecular Devices LLC
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Molecular Devices LLC
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    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0052—Optical details of the image generation
    • G02B21/0056—Optical details of the image generation based on optical coherence, e.g. phase-contrast arrangements, interference arrangements
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B27/00—Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00
    • G02B27/48—Laser speckle optics
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/06—Means for illuminating specimens
    • G02B21/08—Condensers
    • G02B21/086—Condensers for transillumination only
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B7/00—Mountings, adjusting means, or light-tight connections, for optical elements
    • G02B7/28—Systems for automatic generation of focusing signals
    • G02B7/36—Systems for automatic generation of focusing signals using image sharpness techniques, e.g. image processing techniques for generating autofocus signals
    • G02B7/365—Systems for automatic generation of focusing signals using image sharpness techniques, e.g. image processing techniques for generating autofocus signals by analysis of the spatial frequency components of the image
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T5/00—Image enhancement or restoration
    • G06T5/90—Dynamic range modification of images or parts thereof
    • G06T5/94—Dynamic range modification of images or parts thereof based on local image properties, e.g. for local contrast enhancement
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06V—IMAGE OR VIDEO RECOGNITION OR UNDERSTANDING
    • G06V20/00—Scenes; Scene-specific elements
    • G06V20/60—Type of objects
    • G06V20/69—Microscopic objects, e.g. biological cells or cellular parts
    • G06V20/693—Acquisition
    • H—ELECTRICITY
    • H04—ELECTRIC COMMUNICATION TECHNIQUE
    • H04N—PICTORIAL COMMUNICATION, e.g. TELEVISION
    • H04N23/00—Cameras or camera modules comprising electronic image sensors; Control thereof
    • H04N23/56—Cameras or camera modules comprising electronic image sensors; Control thereof provided with illuminating means
    • H—ELECTRICITY
    • H04—ELECTRIC COMMUNICATION TECHNIQUE
    • H04N—PICTORIAL COMMUNICATION, e.g. TELEVISION
    • H04N23/00—Cameras or camera modules comprising electronic image sensors; Control thereof
    • H04N23/60—Control of cameras or camera modules
    • H04N23/67—Focus control based on electronic image sensor signals
    • H04N23/671—Focus control based on electronic image sensor signals in combination with active ranging signals, e.g. using light or sound signals emitted toward objects
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/24—Base structure
    • G02B21/241—Devices for focusing
    • G02B21/244—Devices for focusing using image analysis techniques
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B27/00—Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00
    • G02B27/09—Beam shaping, e.g. changing the cross-sectional area, not otherwise provided for
    • G02B27/0905—Dividing and/or superposing multiple light beams
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B5/00—Optical elements other than lenses
    • G02B5/02—Diffusing elements; Afocal elements
    • G02B5/0273—Diffusing elements; Afocal elements characterized by the use
    • G02B5/0294—Diffusing elements; Afocal elements characterized by the use adapted to provide an additional optical effect, e.g. anti-reflection or filter

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  • Physics & Mathematics (AREA)
  • Engineering & Computer Science (AREA)
  • General Physics & Mathematics (AREA)
  • Optics & Photonics (AREA)
  • Multimedia (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Signal Processing (AREA)
  • Theoretical Computer Science (AREA)
  • Computer Vision & Pattern Recognition (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Health & Medical Sciences (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
  • Microscoopes, Condenser (AREA)
  • Automatic Focus Adjustment (AREA)
  • Eye Examination Apparatus (AREA)
  • Focusing (AREA)
CN201780080905.6A 2016-10-26 2017-10-24 使用干涉条纹增强对比度并发现焦点的透射照明成像 Active CN110114654B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US15/335,032 US10302923B2 (en) 2016-10-26 2016-10-26 Trans-illumination imaging with use of interference fringes to enhance contrast and find focus
US15/335,032 2016-10-26
PCT/US2017/057962 WO2018081037A1 (en) 2016-10-26 2017-10-24 Trans-illumination imaging with use of interference fringes to enhance contrast and find focus

Publications (2)

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CN110114654A CN110114654A (zh) 2019-08-09
CN110114654B true CN110114654B (zh) 2022-10-11

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US (1) US10302923B2 (https=)
EP (2) EP3532822B1 (https=)
JP (2) JP7277051B2 (https=)
CN (1) CN110114654B (https=)
WO (1) WO2018081037A1 (https=)

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WO2019187420A1 (ja) * 2018-03-26 2019-10-03 株式会社Ｓｃｒｅｅｎホールディングス 画像処理方法、画像処理装置、プログラムおよび記録媒体
JP7230409B2 (ja) * 2018-10-02 2023-03-01 カシオ計算機株式会社 判定装置、判定方法及び判定プログラム
JP7283324B2 (ja) * 2019-09-18 2023-05-30 株式会社島津製作所 欠陥検査装置
KR102861065B1 (ko) * 2022-10-12 2025-09-17 (주)힉스컴퍼니 디지털 홀로그래픽 현미경의 자동화를 위한 간섭 패턴 최적화 레퍼런스 빔 경로 길이 자동 조정 방법 및 그 장치
KR102780556B1 (ko) * 2022-10-12 2025-03-12 (주)힉스컴퍼니 디지털 홀로그래픽 현미경의 빔 세기와 빔 경로 길이를 조절하는 방법 및 장치
CN118501051B (zh) * 2024-05-15 2025-03-04 上海默乐光检科技有限公司 一种物镜后焦面对准方法及装置

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WO2015151461A1 (ja) * 2014-04-01 2015-10-08 株式会社ニコン 超解像観察装置及び超解像観察方法
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Also Published As

Publication number Publication date
EP4242630A3 (en) 2023-10-25
EP4242630B1 (en) 2025-12-03
JP7277051B2 (ja) 2023-05-18
JP7549691B2 (ja) 2024-09-11
WO2018081037A1 (en) 2018-05-03
EP3532822A4 (en) 2020-10-07
EP4242630A2 (en) 2023-09-13
JP2023060003A (ja) 2023-04-27
US20180113290A1 (en) 2018-04-26
CN110114654A (zh) 2019-08-09
US10302923B2 (en) 2019-05-28
EP3532822A1 (en) 2019-09-04
EP3532822B1 (en) 2023-05-31
JP2019536085A (ja) 2019-12-12

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