CN110100712A - A kind of method for culturing seedlings inhibiting Fructus Aurantii seedling blight - Google Patents

A kind of method for culturing seedlings inhibiting Fructus Aurantii seedling blight Download PDF

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Publication number
CN110100712A
CN110100712A CN201910440360.6A CN201910440360A CN110100712A CN 110100712 A CN110100712 A CN 110100712A CN 201910440360 A CN201910440360 A CN 201910440360A CN 110100712 A CN110100712 A CN 110100712A
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CN
China
Prior art keywords
fructus aurantii
culturing seedlings
water
matrix
seedlings according
Prior art date
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Pending
Application number
CN201910440360.6A
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Chinese (zh)
Inventor
杨帆
董美超
周东果
王自然
郭莉娜
王绍华
李进学
高俊燕
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INSTITUTE OF TROPICAL AND SUBTROPICAL CASH CROP YUNNAN ACADEMY OF AGRICULTURAL SCIENCES
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INSTITUTE OF TROPICAL AND SUBTROPICAL CASH CROP YUNNAN ACADEMY OF AGRICULTURAL SCIENCES
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Priority to CN201910440360.6A priority Critical patent/CN110100712A/en
Publication of CN110100712A publication Critical patent/CN110100712A/en
Pending legal-status Critical Current

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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01CPLANTING; SOWING; FERTILISING
    • A01C1/00Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G13/00Protecting plants
    • A01G13/02Protective coverings for plants; Coverings for the ground; Devices for laying-out or removing coverings
    • A01G13/0256Ground coverings
    • A01G13/0268Mats or sheets, e.g. nets or fabrics
    • A01G13/0275Films
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/20Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
    • A01G24/22Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
    • A01G24/25Dry fruit hulls or husks, e.g. chaff or coir
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/20Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
    • A01G24/28Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing peat, moss or sphagnum
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G31/00Soilless cultivation, e.g. hydroponics

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Soil Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Toxicology (AREA)
  • Pretreatment Of Seeds And Plants (AREA)
  • Cultivation Of Plants (AREA)

Abstract

The present invention relates to a kind of method for culturing seedlings for inhibiting Fructus Aurantii seedling blight, belong to Fructus Aurantii stock seedling-raising technique field.Method for culturing seedlings of the present invention is the following steps are included: under conditions of taking shelter from rain, the matrix for 20~25cm thickness that tile on seedbed, the Fructus Aurantii seed after sowing sterilized vernalization, then covers the matrix of 1~1.5cm thickness, is compacted be watered with water, overlay film, progress nursery culture;The matrix includes the mixture that mass ratio is the coco bran of 6:3:1, turf and organic fertilizer.Method for culturing seedlings of the present invention can be avoided the generation of Fructus Aurantii seedling blight, improve Fructus Aurantii seedling planting percent, reduce nursery stock production cost.

Description

A kind of method for culturing seedlings inhibiting Fructus Aurantii seedling blight
Technical field
The present invention relates to Fructus Aurantii stock seedling-raising technique fields, and in particular to a kind of nursery side for inhibiting Fructus Aurantii seedling blight Method.
Background technique
The mode of global citrus good seed breeding is grafting and breeding, and for Fructus Aurantii because resistant strong, early fruiting and high yield is good, The features such as fruit quality is higher is one of the advantage stock of current most of Citrus Cultivars.
In reproductive process, damping-off is a kind of higher disease of common and disease incidence of Fructus Aurantii seedling, has onset speed Fastly, disease incidence is high, the feature for causing death of seedling rate high.The disease is caused by a variety of fungies, wherein based on Rhizoctonia solani Kuhn, hair Young stem and rootstock are mainly encroached on when sick, initial stage is crineous, water soaked spots, and upper blade is wilted rapidly, then in green withered shape It is wilting, cause one Cong Cong of nursery seedling, blocks of withered.
At present production on breed Fructus Aurantii seedling matrix mostly with non-sterile native country, river sand, organic fertilizer according to a certain percentage It is bred under open-air atmosphere after mixing, damping-off easily occurs when Fructus Aurantii seedling grows into 3~5 true leaves, cause nursery stock big Area is dead, reduces planting percent, seriously affects nursery stock production, leads to that the production cost increases.It is aobvious according to many years production data statistics Show: damping-off death, such as seedbed are occurred using the Fructus Aurantii seedling that the method for traditional matrices breeding Fructus Aurantii seedling will lead to 25% or so It reuses, disease incidence is up to 50% or more.Therefore current method for culturing seedlings makes damping-off disease caused by Fructus Aurantii seedling Evil can not be effectively suppressed.
Summary of the invention
The purpose of the present invention is to provide a kind of method for culturing seedlings for inhibiting Fructus Aurantii seedling blight.Method for culturing seedlings of the present invention It can reduce Fructus Aurantii seedling blight, improve Fructus Aurantii seedling planting percent, reduce nursery stock production cost.
The present invention provides a kind of method for culturing seedlings for inhibiting Fructus Aurantii seedling blight, comprising the following steps:
Under conditions of taking shelter from rain, the matrix for 20~25cm thickness that tiles on seedbed, the Fructus Aurantii kind after sowing sterilized vernalization Son, then the matrix of 1~1.5cm thickness is covered, compacting is watered with water, and overlay film carries out nursery culture;
The matrix includes the mixture that mass ratio is the coco bran of 6:3:1, turf and organic fertilizer.
Preferably, the method taken shelter from rain includes building greenhouse.
Preferably, the coco bran is the coco bran after high-temperature sterilization.
Preferably, the coco bran is the coco bran after water suction is loose.
Preferably, the width of the matrix tiling is 1~1.5m.
Preferably, the germination accelerating method is the following steps are included: the seed after disinfection is impregnated in 50 DEG C of water Water temperature is adjusted to 55 DEG C by 10min, is continued to impregnate 30min, is pulled out and drain.
Preferably, the density of sowing of the Fructus Aurantii seed is 8000~10000 Li/㎡.
Preferably, the water include phreatic water, tap water or it is sterilized after water.
Preferably, the overlay film is that plastic film is laid in matrix, surrounding compacting.
The present invention provides a kind of method for culturing seedlings for inhibiting Fructus Aurantii seedling blight.The present invention is by improving educating for Fructus Aurantii stock Seedling matrix formulations and the method for management perfection measure improve Fructus Aurantii nursery stock planting percent to realize, and reduce enterprise's production cost.This The method for culturing seedlings is invented to be able to solve in production now using Fructus Aurantii nursery stock production caused by the method for culturing seedlings of traditional matrices In the process because the problem of nursery stock caused by infecting damping-off is dead, increase enterprise's production cost, to improve Fructus Aurantii nursery stock seedling Rate, finally improves the production efficiency of excellent virus-free healthy citrus seedling, while reducing enterprise's production cost, improves enterprise key Competitiveness.
With native country when tradition breeding Fructus Aurantii seedling: husky: organic fertilizer=7:2:1 ratio is mixed into seedling medium in open-air condition Lower breeding, it is dead that the Fructus Aurantii seedling that this method will lead to 25% or so infects fungi generation damping-off when growing into 3~5 true leaves It dies.Implementation through the invention has prevented seedling medium and has carried disease germs the possibility for infecting damping-off and propagating germ through rainwater, and increased The porousness for adding matrix improves the porosity and the content of organic matter of matrix, is conducive to Fructus Aurantii seedling growth and develops, thus significantly The planting percent for improving Fructus Aurantii nursery stock, reduces enterprise's seedling cost.
Specific embodiment
The present invention provides a kind of method for culturing seedlings for inhibiting Fructus Aurantii seedling blight, comprising the following steps:
Under conditions of taking shelter from rain, the matrix for 20~25cm thickness that tiles on seedbed, the Fructus Aurantii kind after sowing sterilized vernalization Son, then the matrix of 1~1.5cm thickness is covered, compacting is watered with water, and overlay film carries out nursery culture;
The matrix includes the mixture that mass ratio is the coco bran of 6:3:1, turf and organic fertilizer.
In the present invention, the method taken shelter from rain includes building greenhouse.
In the present invention, the coco bran is the coco bran after high-temperature sterilization.
In the present invention, the coco bran is the coco bran after water suction is loose.In the present invention, after the water preferably sterilizes Water.
The present invention is not particularly limited the method for the disinfection, using routine disinfection side well known to those skilled in the art Method.In the present invention, the method for the vernalization preferably includes following steps: water temperature being first adjusted to 50 DEG C, pours into Fructus Aurantii Water temperature is adjusted to 55 DEG C after ten minutes and impregnated again 30 minutes, then pulled seed out and drain away the water by seed, immersion.In the present invention, The method of the disinfection is preferred are as follows: is diluted at 500 times of liquid Steeping and buddings with 800 times of liquid of thiophanate methyl Dilution for powder and carbendazim The seed of reason 12 hours, after pull seed out and drain moisture after being rinsed with clear water, in case sowing is used.
In the present invention, the width of the matrix tiling is 1~1.5m, preferably 1.2m.The present invention prior to seeding, preferably Bed surface is struck off and is compacted wait sow.
In the present invention, the density of sowing of the Fructus Aurantii seed is 8000~10000 Li/㎡, preferably 8500~9500 Li/㎡.
In the present invention, the water include phreatic water, tap water or it is sterilized after water.
In the present invention, the overlay film is that plastic film is laid in matrix, surrounding compacting.
Preferably after germination is unearthed, overlay film is opened by the present invention, with the 600 times of liquid sprays of first frost hymexazol or carbendazim Leaching, and flat membrane is changed to arch membrane, surrounding compacting in time.Every 10 days are primary with first frost hymexazol or 600 times of liquid sprays of carbendazim, When nursery stock it is long to 4~5 true leaves when start hardening, hardening first day can be after 18:00 temperature slightly reduces in the afternoon every moisture in the soil arch membrane End is opened, again that every moisture in the soil arch membrane is fully open after 3~4 days.Can be out of the garden transplanting after 10 days.
Combined with specific embodiments below to it is of the present invention it is a kind of inhibit Fructus Aurantii seedling blight method for culturing seedlings do into one Detailed introduction is walked, technical solution of the present invention includes but is not limited to following embodiment.
Embodiment 1
1, matrix prepares
It will first be sprayed by the compression coco bran after high temperature sterilization with tap water or sterile-processed water, make it sufficiently Water suction, after its sufficiently absorb water it is completely loose after, coco bran, turf and organic fertilizer is added in the ratio of 6:3:1 by volume, and mixes It is even, it has deposited spare;
2, Fructus Aurantii seed treatment
(1) water temperature is first adjusted to 50 DEG C by vernalization, pours into Fructus Aurantii seed, and water temperature is adjusted to 55 DEG C again after ten minutes by immersion It impregnates 30 minutes, then pulls seed out and drain away the water;
(2) it sterilizes, dilutes 500 times of liquid Steeping and budding processing with 800 times of liquid of thiophanate methyl Dilution for powder and carbendazim Seed 12 hours, after pull seed out and drain moisture after being rinsed with clear water, in case sowing is used;
3, seedbed preparation
In the environment of taking shelter from rain, the matrix got ready tiling is the seedbed of 1.2 meters wide 20~25cm thickness, and bed surface is struck off It is compacted wait sow.
4, it sows
It by the seed handled well with 8000~10000/square metre, then is covered with this matrix of 1~1.5cm thickness, compacting Be watered with tap water or it is sterile-processed after water, by plastic film tile seedbed on, surrounding compacting.
Method of the present invention at after planting 25 days or so, be unearthed by germination, and plastic film is opened, is disliked with first frost 600 times of liquid sprays of mould spirit or carbendazim, and flat membrane is changed to arch membrane, surrounding compacting in time.Every 10 days with first frost hymexazol or Carbendazim 600 times of liquid spray is primary, when nursery stock it is long to 4~5 true leaves when start hardening, hardening first day can 18:00 in the afternoon Temperature opens every moisture in the soil arch membrane end after slightly reducing, again that every moisture in the soil arch membrane is fully open after 3~4 days.It can be out of the garden after 10 days Transplanting.
Sowing on November 7th, 2018, on December 2nd, 2018 (germination is unearthed) replacement arch membrane, 13 sunrise January in 2019 Withered seedling is now found, damping-off onset peak period appears on January 30th, 2019, the same day and investigation statistics damping-off incidence, knot Fruit such as table 1.
Traditional matrices type of seeding are as follows: with native country: husky: organic fertilizer=7:2:1 ratio is mixed into seedling medium outdoor Under the conditions of breed.
The sowing of 1 traditional matrices of table, take shelter from rain sowing and non-take shelter from rain of matrix of the present invention under management condition of matrix of the present invention manage item The Fructus Aurantii seedling sowed under part, in after planting 83 days damping-off onset peak period investigation statistics results
It can be seen from Table 1 that matrix of the present invention+management of taking shelter from rain has been effectively prevented Fructus Aurantii seedling and has infected damping-off in seedling stage The case where, Fructus Aurantii seedling planting percent is significantly improved, to improve the production efficiency of excellent virus-free healthy citrus seedling, reduces enterprise Production cost is improved enterprises' comprehensive competitiveness.
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications are also answered It is considered as protection scope of the present invention.

Claims (9)

1. a kind of method for culturing seedlings for inhibiting Fructus Aurantii seedling blight, comprising the following steps:
Under conditions of taking shelter from rain, the matrix for 20~25cm thickness that tiles on seedbed, the Fructus Aurantii seed after sowing sterilized vernalization, then The matrix of 1~1.5cm thickness is covered, compacting is watered with water, and overlay film carries out nursery culture;
The matrix includes the mixture that mass ratio is the coco bran of 6:3:1, turf and organic fertilizer.
2. method for culturing seedlings according to claim 1, which is characterized in that the method taken shelter from rain includes building greenhouse.
3. method for culturing seedlings according to claim 1, which is characterized in that the coco bran is the coco bran after high-temperature sterilization.
4. method for culturing seedlings according to claim 1 or 3, which is characterized in that the coco bran is the coco bran after water suction is loose.
5. method for culturing seedlings according to claim 1, which is characterized in that the width of the matrix tiling is 1.0~1.5m.
6. method for culturing seedlings according to claim 1, which is characterized in that the method for the vernalization will be the following steps are included: will disappear Seed after poison impregnates 10min in 50 DEG C of water, and water temperature is adjusted to 55 DEG C, continues to impregnate 30min, pulls out and drain.
7. method for culturing seedlings according to claim 1, which is characterized in that the Fructus Aurantii seed sow density be 8000~ 10000 Li/㎡.
8. method for culturing seedlings according to claim 1, which is characterized in that the water includes phreatic water, tap water or sterilized Water afterwards.
9. method for culturing seedlings according to claim 1, which is characterized in that the overlay film is that plastic film is laid in matrix On, surrounding compacting.
CN201910440360.6A 2019-05-24 2019-05-24 A kind of method for culturing seedlings inhibiting Fructus Aurantii seedling blight Pending CN110100712A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110367107A (en) * 2019-08-30 2019-10-25 云南省农业科学院热带亚热带经济作物研究所 A kind of method for culturing seedlings of Fructus Aurantii seedling

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JP2008127366A (en) * 2006-11-24 2008-06-05 Kumiai Chem Ind Co Ltd Agricultural and horticultural composition
CN107646469A (en) * 2017-09-28 2018-02-02 田阳县群姐砂糖橘专业合作社 A kind of method of granulated sugar tangerine insect prevention
CN109452127A (en) * 2019-01-07 2019-03-12 云南省农业科学院热带亚热带经济作物研究所 It is a kind of improve Fructus Aurantii emergence rate seedling medium and its application
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110367107A (en) * 2019-08-30 2019-10-25 云南省农业科学院热带亚热带经济作物研究所 A kind of method for culturing seedlings of Fructus Aurantii seedling

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