CN109983932A - A method of promoting grape cuttage root-taking using passion fruit extracting solution - Google Patents
A method of promoting grape cuttage root-taking using passion fruit extracting solution Download PDFInfo
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- CN109983932A CN109983932A CN201711482672.0A CN201711482672A CN109983932A CN 109983932 A CN109983932 A CN 109983932A CN 201711482672 A CN201711482672 A CN 201711482672A CN 109983932 A CN109983932 A CN 109983932A
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- passion fruit
- cuttings
- root
- extracting solution
- cuttage
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/02—Cultivation of hops or vines
Abstract
The present invention relates to a kind of methods for promoting grape cuttage root-taking using passion fruit extracting solution, comprise the following steps that step S1, the preparation of passion fruit extracting solution；Step S2, the preparation of grape stalk；Rapid S3, cuttings is bundled, and lower end, which is placed in passion fruit extracting solution, impregnates 24-36h；Step S4 takes out the cuttings after impregnating, is rinsed with clear water, prepare cuttage after drying；Step S5, the preparation of slotting machine；Step S6, cuttage；Slotting machine is placed in ventilation by step S7, controls temperature at 20-25 DEG C, and the matrix of slotting machine is made to keep moisture state；Step S8 after three months, is grown to grape cuttage seeding adventitious root, is transplanted after root system more prosperity.Using this method, the cuttage seeding root system of acquisition is more developed, and fibrous root quantity and length, new-tip length and cuttage survival rate are substantially better than control and the processing of ABT root-inducing powder, and also provide a new thinking to the research of plant establishment mechanism.
Description
Technical field
The present invention relates to technical field of agricultural seedling-raising, and in particular to a kind of to promote grape cuttage raw using passion fruit extracting solution
The method of root.
Background technique
The cuttage of plant generally promotes it to take root using growth-promoting root parahormone at present, to obtain higher cuttage survival rate.
But occasionally there are bigger differences for the complexity of its individual cuttage root-taking of different plants even kindred plant different cultivars, especially
It is the difficult comparatively ideal effect of acquirement using root-growing agent for the plant for being difficult to cuttage root-taking.This is just needed by a variety of
Means comprehensively utilize to improve its rooting rate.Have at present multiple using plant extraction liquid or extracting solution and other chemical components
Combination formula promotes the report of cuttage root-taking, but generally existing drug used is various, configuration is complicated and higher cost etc. is asked
Topic.
Grape also has relatively big difference because of the survival rate of type or its different hardwood cutting of kind.Especially in grape production
Commonly " huge peak ", " rose fragrant " class grape, cuttage survival rate is generally lower, using the quick root dipping of the root-growing agent of high concentration or
The method that the root-growing agent of low concentration impregnates for a long time can promote it to take root to a certain extent, but effect is very unstable, not of the same race
The grape of class or kind is larger to the sensitivity differences of rooting parahormone concentration and processing time, and exogenous hormone is excessively used
It is easy to upset the physiological equilibrium of cuttings, the subsequent nutrient growth of cuttings is impacted, dead seedling or growing way are be easy to cause after transplanting
It is weaker, limit application and popularization of the cuttage technique in grape production.
Summary of the invention
In conclusion in order to overcome the deficiencies of the prior art, technical problem to be solved by the invention is to provide a kind of utilizations
The method that passion fruit extracting solution promotes grape cuttage root-taking, can significantly improve the hardwood cutting rooting rate of difficult cuttage grape,
Root system after taking root is more flourishing, and transplanting survival rate is high, grows vigorous.This method be cultivate excellent dedicated grape rootstock kind and
Foundation for grape cuttage and quick-propagation production technology is laid a good foundation.
The technical scheme to solve the above technical problems is that a kind of promote grape cuttage using passion fruit extracting solution
The method taken root, comprises the following steps that
Step S1, the preparation of passion fruit extracting solution: selection health and full ripe passion fruit are placed on ventilating and cooling place
Saccharification 7-10 days is put into oven drying after surface is slightly in yellow；The passion fruit of drying is broken into pieces with one's fingers again, then by fruit juice together with
Pericarp is immersed in together in sterile hot water, is impregnated, is stirred therebetween for several times, finally in the environment of the sterile hot water Temperature fall
It is spare to obtain passion fruit extracting solution for filtering and removing slag；
Step S2, the preparation of grape stalk: setting mid-October in late April, and selection growth is vigorous, bud eye is full, disease-free
Life in 1 year of insect pest or 2 years raw grapevine branches are cut into long 16-20cm and inserting containing 2-3 full buds as cuttings, by cuttings
Fringe；It is cut flat at position of the cuttings upper end from terminal bud 1-1.5cm, lower end is cut into inclined-plane at the position far from bottom bud 0.7-1cm；
Step S3, cuttings is bundled, and lower end, which is placed in passion fruit extracting solution, impregnates 24-36h；
Step S4 takes out the cuttings after impregnating, is rinsed with clear water, prepare cuttage after drying；
Step S5, the preparation of slotting machine: the preparation of slotting machine carries out in the greenhouse, spreads the treasure of one layer of 5-10cm in bubble chamber bottom
Then Zhu Yan fills up sandy soil mixture that carbendazim sterilized as matrix, drainage hole and air hole is opened up on bubble chamber；
Cuttage: step S6 makes cuttings lower end inclined-plane upwards with slotting machine plane in 20 ° of angles, is inserted into the 2/3 of training cuttings, phase
The spacing of adjacent cuttings is 8-12cm；Cuttings is equably sprayed water and is poured to its top notch sealing wax, then in matrix after completing cuttage
Thoroughly, grape cuttage seeding is obtained；
Slotting machine is placed in ventilation by step S7, controls temperature at 20-25 DEG C, and the matrix of slotting machine is made to keep moisture state；
Step S8, after three months, the adventitious root to grape cuttage seeding is grown, and is transplanted after root system more prosperity.
Studies have shown that adding some substances such as sucrose, vitamin C, amino-acid salt etc. in rooting process has one to taking root
Fixed facilitation.Cuttings consumed nutriment in rooting process is mainly carbohydrate and nitrogen compound, it
Not only provide cuttage rooting and growth nutriment, while be also cuttings base portion healing and new root energy substance.
C/N value can be well reflected its rootability in cuttings, and the bigger rootability of C/N value is stronger in a certain range, and cuttings is in life
Nutriment and other matter interactions, jointly promote the generation and growth of adventitious root during root.Passion fruit extracting solution
In rich in glucoside, fructose, glucose, protein, lipid etc. and minerals and vitamins abundant etc., the wherein sweet tea of passion fruit glycosides
Degree is 300 times of sucrose or more.Obviously, metabolite special in passion fruit body plays facilitation to taking root.
The beneficial effects of the present invention are: grape variety " huge peak " and " rose of more difficult cuttage can be made using this method
The hardwood cutting survival rate of perfume " is respectively increased to 70% and 80% or more.The cuttage seeding root system of acquisition is more developed, fibrous root quantity and
Length, new-tip length and cuttage survival rate are substantially better than control and the processing of ABT root-inducing powder.Seedling is easy after transplanting, and growing way is good.Cause
The extracting solution that edible single passion fruit fruit is only used for this method does not add other biochemical reagents as growth-promoting root reagent
Ingredient, it is simple and easy to use, securely and reliably.The hormones composition such as IAA, IBA, NAA containing facilitation of taking root is not added, no yet
Its normal physiological growth can be influenced because root-growing agent concentration improper use causes the hormone imbalances of cuttings.This method not only has
Biggish application practice meaning also provides a new thinking to the research of plant establishment mechanism.
Based on the above technical solution, the present invention can also be improved as follows:
Further, in step S1 passion fruit furnace drying method are as follows: 50 DEG C are improved temperature after low-temperature bake 2-3 days and dried to 65 DEG C
It is 2 days roasting, it then cools to 55 DEG C and continues baking 2-3 days, when the weight of fruit drops to the 25-30% of suitable fresh fruit weight,
Temperature fall obtains drying dried nuts.
Further, the temperature of sterile hot water described in step S1 is 80-90 DEG C, and weight is 30 times of passion fruit；It impregnates
Time be 12-16h.
Further, the inclined-plane of cuttings lower end and terminal bud are in the same side of cuttings in step S2.
Further, sandy soil mixture includes rough sand and loam in step S5, and the weight ratio of the rough sand and loam is 2:1.
Further, in step S5 there are two drainage holes, a diameter of 1cm and the bottom for being in bubble chamber；Air hole has 4-5
A, a diameter of 1cm is simultaneously at four side walls of bubble chamber and the position apart from bottom 5-7cm.
Specific embodiment
Principles and features of the present invention are described below in conjunction with specific example, example is served only for explaining this hair
It is bright, it is not intended to limit the scope of the present invention.
Embodiment 1
(1) preparation of passion fruit extracting solution: one health of selection and full ripe passion fruit place ventilating and cooling place sugar
Change 7-10 days, oven drying drying is put into after surface is slightly in yellow, breaks into pieces with one's fingers, fruit juice is immersed in together with pericarp
About 16h in 700mL, 90 DEG C of sterile hot water.It is spare to retain passion fruit extracting solution for filtering and removing slag.
(2) preparation of grape branch: setting mid-October in late April, and selection growth is vigorous, bud eye is full, no disease and pests harm
Life in 1 year or life in 2 years " huge peak " and " rose fragrant " grapevine branch as cuttings, cuttings is cut into long 16-20cm and containing 2-3
The cuttings of full bud；It is cut flat at position of the cuttings upper end from terminal bud 1-1.5cm, lower end is cut at the position far from bottom bud 0.7-1cm
At inclined-plane (in the identical faces of cuttings terminal bud).
(3) cuttings is bundled, lower end, which is placed in passion fruit extracting solution, impregnates 30h.
(4) cuttings after impregnating is taken out, and is rinsed with clear water, prepares cuttage after slightly drying.
(5) preparation of slotting machine: the preparation of slotting machine carries out in the greenhouse, using being about 55cm, width about 40cm, high about 20cm
Bubble chamber, bottom spread the perlite of one layer of 5-10cm or so, then fill up sandy soil mixture (rough sand and earth that carbendazim sterilized
Local soil type is at the weight ratio of the two is 2:1).The apopore of 2 diameter 1cm or so is opened in the bottom of bubble chamber, the four of bubble chamber
The ventilation mouth of 4-5 diameter 1cm or so is opened respectively in position of a side wall apart from bottom 5-7cm.
(6) cuttage.Keep cuttings lower end inclined-plane upward, is in 20 ° of angles with slotting machine plane, the 2/3 of insertion training cuttings, between cuttings
Away from for 10cm.After cuttings has been inserted, by cuttings top notch sealing wax.
(7) it is equably sprayed water, is irrigated in matrix with kettle.Slotting machine is placed in ventilation, controls temperature on a 20-25 DEG C of left side
It is right.Frequent water drenching makes slotting machine keep wet.
(8) count and transplant: after three months, rooting rate, adventitious root quantity, root length and the young sprout for investigating cuttage seeding are long
Degree.Potted plant growth in peat soil matrix is transplanted to after the completion of statistics.
Embodiment 2
The reagent of taking root used is ABT root-inducing powder: 1g ABT root-inducing powder is dissolved in 50ml alcohol, is stirred well to completely molten
Solution, is diluted to concentration 2g/L with sterile water.By the cuttings handled well quickly dipped 5 seconds in the solution after dilution, rapid cuttage in
In culture substrate.Remaining process is the same as embodiment 1.
Embodiment 3
The ABT root-inducing powder concentration used is 0.2g/L, cuttings is impregnated to cuttage after 6h in the solution, remaining is the same as embodiment 3.
Embodiment 4
It handles without using any solution or reagent, is directly inserted on slotting machine after cuttings processing.Remaining operation is the same as embodiment 1.
Table 1 shows " mean elements ", " average root of the 4 kinds of processing modes of embodiment 1-4 to two kinds of grape cuttage root-takings
The influence of length ", " average new-tip length " and " root survival ".
Influence of the 1 different disposal method of table to " huge peak " and " rose fragrant " grape cuttage survival rate
As can be seen from Table 1, after being impregnated using passion fruit extracting solution to cuttings, the mean elements of two grape varieties is put down
Equal root long, average new-tip length and root survival are all higher than non-treated control and using the processing controls of ABT root-inducing powder.Wherein most
It is apparent that being significantly higher than other processing on mean elements and cuttage survival rate.And use different ABT root-inducing powder processing modes
The result shows that, the quickly dipped rooting efficiency of high concentration will significantly better than low concentration impregnate effect.Low concentration ABT root-inducing powder impregnates
Effect be not significantly different with non-treated compare, illustrate ABT root-inducing powder difference using concentration and Different treatments to grape
Cuttage root-taking is affected.The complexity of taking root of " huge peak " and " rose fragrant " grape has relatively big difference, but passes through passion fruit
Comparatively ideal root survival has been obtained after extracting solution immersion treatment, illustrates the processing of passion fruit extracting solution to grape cuttage root-taking
With obvious facilitation, thus demonstrate " passion fruit extracting solution can remarkably promote vine hard branch cuttage root-taking " this
Conclusion.
The foregoing is merely presently preferred embodiments of the present invention, is not intended to limit the invention, it is all in spirit of the invention and
Within principle, any modification, equivalent replacement, improvement and so on be should all be included in the protection scope of the present invention.
Claims (6)
1. a kind of method for promoting grape cuttage root-taking using passion fruit extracting solution, which is characterized in that comprise the following steps that
Step S1, the preparation of passion fruit extracting solution: selection health and full ripe passion fruit are placed on ventilating and cooling place saccharification
7-10 days, oven drying is put into after surface is slightly in yellow；The passion fruit of drying is broken into pieces with one's fingers again, then by fruit juice together with pericarp
It is immersed in sterile hot water together, is impregnated in the environment of the sterile hot water Temperature fall, stir for several times, finally filter therebetween
Slagging-off, it is spare to obtain passion fruit extracting solution；
Step S2, the preparation of grape stalk: in late April to mid-October, selection growth is vigorous, bud eye is full, no disease and pests harm
Life in 1 year or 2 years raw grapevine branches as cuttings, cuttings is cut into long 16-20cm and cuttings containing 2-3 full buds；?
Cuttings upper end is cut flat at the position from terminal bud 1-1.5cm, and lower end is cut into inclined-plane at the position far from bottom bud 0.7-1cm；
Step S3, cuttings is bundled, and lower end, which is placed in passion fruit extracting solution, impregnates 24-36h；
Step S4 takes out the cuttings after impregnating, is rinsed with clear water, prepare cuttage after drying；
Step S5, the preparation of slotting machine: the preparation of slotting machine carries out in the greenhouse, spreads the pearl of one layer of 5-10cm in bubble chamber bottom
Then rock fills up sandy soil mixture that carbendazim sterilized as matrix, drainage hole and air hole is opened up on bubble chamber；
Step S6, cuttage: making cuttings lower end inclined-plane upwards with slotting machine plane in 20 ° of angles, be inserted into the 2/3 of training cuttings, adjacent to insert
The spacing of fringe is 8-12cm；Cuttings is equably sprayed water and is irrigated to its top notch sealing wax, then in matrix after completing cuttage, obtains
To grape cuttage seeding；
Slotting machine is placed in ventilation by step S7, controls temperature at 20-25 DEG C, and the matrix of slotting machine is made to keep moisture state；
Step S8, after three months, the adventitious root to grape cuttage seeding is grown, and is transplanted after root system more prosperity.
2. the method according to claim 1 for promoting grape cuttage root-taking using passion fruit extracting solution, which is characterized in that step
The furnace drying method of passion fruit in rapid S1 are as follows: 50 DEG C improve after low-temperature bake 2-3 days temperature to 65 DEG C baking 2 days, then cool to
55 DEG C are continued baking 2-3 days, and when the weight of fruit drops to the 60-70% of fresh fruit weight, Temperature fall obtains drying dried nuts.
3. the method according to claim 1 for promoting grape cuttage root-taking using passion fruit extracting solution, which is characterized in that step
The temperature of sterile hot water described in rapid S1 is 80-90 DEG C, and weight is 30 times of passion fruit；The time of immersion is 12-16h.
4. the method according to claim 1 for promoting grape cuttage root-taking using passion fruit extracting solution, which is characterized in that step
The inclined-plane of cuttings lower end and terminal bud are in the same side of cuttings in rapid S2.
5. the method according to claim 1 for promoting grape cuttage root-taking using passion fruit extracting solution, which is characterized in that step
Sandy soil mixture includes rough sand and loam in rapid S5, and the weight ratio of the rough sand and loam is 2:1.
6. the method according to claim 1 for promoting grape cuttage root-taking using passion fruit extracting solution, which is characterized in that step
In rapid S5 there are two drainage holes, a diameter of 1cm and the bottom for being in bubble chamber；Air hole has 4-5, and a diameter of 1cm is simultaneously
At four side walls in bubble chamber and the position apart from bottom 5-7cm.
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| CN106472080A (en) * | 2016-09-29 | 2017-03-08 | 博白县大红火龙果专业合作社 | A kind of cultural method of booth Hylocereus undatuss |
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