CN109912325A - The method of source-separated urine harmless treatment and fermenting organism liquid organic fertilizer - Google Patents
The method of source-separated urine harmless treatment and fermenting organism liquid organic fertilizer Download PDFInfo
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- CN109912325A CN109912325A CN201910229973.5A CN201910229973A CN109912325A CN 109912325 A CN109912325 A CN 109912325A CN 201910229973 A CN201910229973 A CN 201910229973A CN 109912325 A CN109912325 A CN 109912325A
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- urine
- organic fertilizer
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- harmless treatment
- fermenting organism
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Abstract
The present invention relates to a kind of source-separated urine harmless treatment and the methods of fermenting organism liquid organic fertilizer, the technical issues of which solve urine collecting and processing, it includes urine collecting, ozone treatment, seed fermentation, urine fermentive step, obtains liquid bio-organic fertilizer.The present invention can be used for the preparation field of urine harmless treatment and fermenting organism liquid organic fertilizer.
Description
Technical field
The invention belongs to fertilizer productions and ecological, environmental protective technical field, and in particular to a kind of source-separated urine harmless treatment
And the method for fermenting organism liquid organic fertilizer.
Background technique
Urine is to be percolated the complex mixture for coming out and being discharged through urinary system from blood by human kidney.Healthy adult
The urine yield of people for each person every day is about 1~1.5L.In urine components in addition to the moisture containing 97% or so, also containing big
Other substances of amount.These ingredients can substantially be classified as electrolyte, nitrogenous compound, vitamin, hormone, organic acid and other have
Machine compound, wherein the most substance of content has urea, uric acid, various inorganic salts and protein etc..Therefore in human urine
Containing a large amount of nutriment, the double grading with certain pollution and resource.
Shortage of water resources is whole world problem of interest, and the processing mode of the current urine in China is the punching of urine tap water
It washes, is emitted into sewage treatment plant after mixing with other sanitary sewages.From the point of view of ecological, environmental protective, it can be controlled from source
Pollutant separates and collects it and is used, i.e., is individually received in the case where fecaluria not mixed diluting as far as possible
Collect and it is recycled.
Source-separated urine refers to the urine for being separately separated and collecting from source, is different from the side of urine and mixed collection of defecating
Formula.Theoretical according to ecological, environmental protective, recycling and finally revert to farmland source-separated urine is optimal path, thus realization it is low at
Originally, the urine process technology easily stored has become the inexorable trend of technology development, but current China is to urine process technology
It studies less, is much unable to satisfy the following application requirement to the technology, needs the research work for reinforcing this aspect.
The present invention sends out the recycling of the nutriments such as nitrogen, phosphorus and urine process Research progress in source-separated urine
It is bright.
Summary of the invention
The present invention is exactly to provide a provenance point to solve existing urine collecting and the undesirable technical problem for the treatment of effect
From urine harmless treatment and the method for fermenting organism liquid organic fertilizer, the urine of source separation is carried out at ozone harmless
Reason, by microorganism to urine fermentive and deodorization based on, realize the recycling of nitrogen, the nutrients such as phosphorus, production liquid bio is organic
Fertilizer.
For this purpose, the present invention provides a kind of method of source-separated urine harmless treatment and fermenting organism liquid organic fertilizer,
Include the following steps:
(1) urine collecting: urine is collected separately；
(2) it ozone treatment: carries out disinfection and sterilizes using urine of the ozone to collection；
(3) seed fermentation: bacillus subtilis, bacillus amyloliquefaciens, lactobacillus plantarum, saccharomycete are reached through individually fermentation
To 108CFU/mL or more, then 1:1:2:2 is mixed by volume, obtains composite flora.
(4) it urine fermentive: is added into the urine handled by the step (2) compound obtained in the step (3)
Flora and molasses carry out enclosed system anaerobic fermentation under conditions of 25~30 DEG C after mixing, it is organic to obtain liquid bio
Fertilizer.
Preferably, in step (4), raw material weight number includes 450 parts of Pretreatment urine, 10~25 parts of molasses, beneficial
Microorganism fermenting compound fungus mass-sends 15~25 parts of zymotic fluid.
Preferably, in step (4), stirring rate when fermentation is 125~250r/min, fermentation time control for 10~
15d。
Preferably, in step (2), the ozone dosage is 0.6~1.5g/L or more.
The specific method is as follows for the preparation of the compoiste fermented flora of beneficial microbe in step (4):
1, the LB culture medium of high-temperature sterilization processing (121 DEG C, 30min) is quantitative spare, the formula of culture medium is peptone
10g/L, yeast powder 5g/L, sodium chloride 10g/L supply water 1L, agar 20g (liquid is not added), for bacillus inoculation and
Culture.
2, the YPD culture medium of high-temperature sterilization processing (115 DEG C, 20min) is quantitative spare, the formula of culture medium is peptone
20g/L, yeast powder 10g/L, glucose 20g/L supply water 1L, agar 20g (liquid is not added), inoculation and training for yeast
It supports.
3, the MRS culture medium by high-temperature sterilization processing (115 DEG C, 30min) quantifies spare, peptone 10g/L, beef extract
10g/L, yeast extract 5g/L, diammonium hydrogen citrate 2g/L, glucose 20g/L Tween 80 1.0mL sodium acetate 5g/L, phosphoric acid hydrogen two
Potassium 2g/L, magnesium sulfate 0.58g/L, manganese sulfate 0.25g/L, water 1L, agar 20g/L (liquid is not added) are supplied, is used for plant cream bar
The inoculation and culture of bacterium.
4, the strain of activation is respectively connected in fluid nutrient medium 28~30 DEG C, 160r/min oscillation, cultivated 24 hours.
5, cultured bacterium solution is counted, adjusting the bacteria containing amount of four plants of bacterial strains is bacillus subtilis 2*108CFU/mL, solution
Bacillus amyloliquefaciens 1*108CFU/mL, lactobacillus plantarum 5*108CFU/mL, saccharomycete 2*108CFU/mL, then by volume 1:
1:2:2 is mixed, and beneficial microbe fermenting compound fungus group, the i.e. secondary seed solution of urine fermentive are formed.
Specific embodiment
According to following embodiments, the present invention may be better understood.However, as it will be easily appreciated by one skilled in the art that real
It applies content described in example and is merely to illustrate the present invention, without this hair described in claims should will not be limited
It is bright.Strain in the present invention is purchased from Institute of Microorganism, Academia Sinica, including bacillus subtilis CGMCC 1.108, solution
Bacillus amyloliquefaciens CGMCC 1.1099, lactobacillus plantarum CGMCC1.556, Issatchenkia orientalis CGMCC 2.1533.
Embodiment 1: the ozone treatment method of urine is as follows:
Urine measures COD 3120mg/L before urine process after collecting, and displaing yellow has urine to disturb taste；When urine is through ozone
After the dosage processing level for reaching 1.1g/L, COD falls to 876mg/L, close to colorless and odorless；
Embodiment 2: urine organic fertilizer fermentation
450 parts of urine treated by ozone adds 10 parts of beneficial microbe fermenting compound fungus group secondary seed solution and molasses
15 parts, sealed fermenting is carried out, stirring rate is 125~250r/min, and ferment 5d, and liquid bio-organic fertilizer is made.The organic fertilizer
Measure total nutrient (N+P2O5+K2O), 238g/L；Organic matter, 472g/L, pH value (pH) (1:250 times dilutes), 5.8.
Embodiment 3: liquid organic fertilizer is applied to tomato
It selects in Jiangsu Jiangyin growing tomatoes greenhouse test area to be 3 mu.Test sets 3 processing, and control (CK) is no
Application of organic fertilizers, liquid bio-organic fertilizer (M1), application commodity solid organic fertilizer (M2), each processing set 3 repetitions, random area
Group arrangement.The usage amount of organic fertilizer is 2000kg/ mus；It applies liquid bioorganic fertilizer to pour 4 times with water, amounts to 400Kg.
After being colonized 30 d, every processing takes 10 plants at random, measures plant height, stem thickness, blade quantity, and be averaged.It is each to handle when picking time
10 plants are taken at random, measurement single fruit weight, Weight per plant and total output.
After tomato is colonized 30d, Development of Tomato can be promoted by applying liquid bioorganic fertilizer and organic commercial fertilizer.Strain
High aspect, liquid bioorganic fertilizer is similar to organic commercial fertilizer treatment effect, is all higher than control treatment.The number of blade of two processing
It is all apparently higher than control treatment, liquid bioorganic fertilizer and organic commercial fertilizer are close.Thus, it will be seen that application biofluid
Organic fertilizer can effectively facilitate the growth and development (table 1) of tomato.
Influence of 1 liquid bioorganic fertilizer of the table processing to tomato growth and development
| Processing | Plant height (cm) | Stem thickness (cm) | The number of blade |
| CK | 91.6 | 0.64 | 10.6 |
| M1 | 97.5 | 0.95 | 15.6 |
| M2 | 95.6 | 0.93 | 15.5 |
When tomato picking time, compared with the control, the volume increase of liquid bioorganic fertilizer, organic commercial fertilizer to tomato is applied
Effect items index is all higher than control.In terms of Weight per plant, liquid bioorganic fertilizer is compared with organic commercial fertilizer, single fruit weight and single plant
4.7g and 0.09kg are increased again, but are apparently higher than control treatment.And same area output is compared, liquid bioorganic fertilizer processing
Than normal fermentation processing volume increase 9.3kg, increase production 44.4kg than control treatment.Illustrate that applying the liquid bioorganic fertilizer can obviously increase
Add the yield of tomato.(table 2)
Influence of 2 organic compost of table to tomato production
| Processing | Single fruit weight (g) | Weight per plant (Kg) | Yield (Kg) |
| CK | 95 | 2.01 | 241.8 |
| M1 | 115.7 | 2.36 | 286.2 |
| M2 | 109 | 2.23 | 276.9 |
Embodiment 4: liquid organic fertilizer is applied to tealeaves
In jiangsu wuxi organic tea garden base, uniformly experimental field 4 mu or so of tea tree growing way are selected, tea tree and base are tested
The management of other tea trees of ground, only applies fertilizer different.4 pieces are experimental field divided into, respectively corresponds 4 processing, between each processing
There is the isolation strip equipped with 2 meters or so, liquid bio-organic fertilizer is diluted through water and is used, 5 processing are respectively clear water control
(CK), liquid organic fertilizer high concentration (dilution 100 times), concentration (300 times of dilution), liquid organic fertilizer low concentration in liquid organic fertilizer
(1000 times of dilution), pours according to 5 liters every square metre of amounts and uses.Each processing is applied 2 times in winter in an identical manner, spring
Harvest tealeaves, the results showed that the spring tea yield of application 1000 times of low concentrations of dilution compares no significant difference with clear water；It applies highly concentrated
The spring tea yield of degree is suitable, and all than 55% or more clear water control volume increase, the spring tea yield of concentration also compares than clear water and increases in application
Produce 20% or so.
Claims (4)
1. a kind of method of source-separated urine harmless treatment and fermenting organism liquid organic fertilizer, it is characterized in that including following step
It is rapid:
(1) urine collecting: urine is collected separately；
(2) it ozone treatment: carries out disinfection and sterilizes using urine of the ozone to collection；
(3) seed fermentation: bacillus subtilis, bacillus amyloliquefaciens, lactobacillus plantarum, saccharomycete reach through individually fermentation
108CFU/mL or more, then 1:1:2:2 is mixed by volume, obtains composite flora；
(4) composite flora obtained in the step (3) urine fermentive: is added into the urine handled by the step (2)
And molasses, enclosed system anaerobic fermentation is carried out under conditions of 25~30 DEG C after mixing, obtains liquid bio-organic fertilizer.
2. the method for source-separated urine harmless treatment according to claim 1 and fermenting organism liquid organic fertilizer, special
Sign is in the step (4) that raw material weight number includes 450 parts of Pretreatment urine, 10~25 parts of molasses, beneficial microbe
Fermenting compound fungus mass-sends 15~25 parts of zymotic fluid.
3. the method for source-separated urine harmless treatment according to claim 1 or 2 and fermenting organism liquid organic fertilizer,
It is characterized in that in the step (4), stirring rate when fermentation is 125~250r/min, and fermentation time control is 10~15d.
4. the method for source-separated urine harmless treatment according to claim 1 or 2 and fermenting organism liquid organic fertilizer,
It is characterized in that in the step (2), the ozone dosage is 0.6~1.5g/L or more.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110156509A (en) * | 2019-06-27 | 2019-08-23 | 浙江亲水园生物科技有限公司 | A kind of in-situ preparation method of urine organic liquor extensive use of chemical fertilizer |
| CN110937929A (en) * | 2019-12-17 | 2020-03-31 | 江苏豹邦科技发展有限公司 | Anhydrous ecological toilet urine treatment microbial preparation and urine treatment process thereof |
| CN110963834A (en) * | 2019-12-30 | 2020-04-07 | 浙江亲水园生物科技有限公司 | Method for organic liquid fertilization of urine |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1765841A (en) * | 2004-10-26 | 2006-05-03 | 黄海智 | Method for innocent treatment of dung or urine of stock and fowl and utilization |
| CN101081748A (en) * | 2006-05-29 | 2007-12-05 | 天津市汉沽区福祥肥料加工厂 | Animal dung urine handling method |
| CN105272472A (en) * | 2015-11-06 | 2016-01-27 | 海南大学 | Biogas slurry formula fertilizer through fermentation of primary pig manure and urine and preparation method thereof |
-
2019
- 2019-03-26 CN CN201910229973.5A patent/CN109912325A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1765841A (en) * | 2004-10-26 | 2006-05-03 | 黄海智 | Method for innocent treatment of dung or urine of stock and fowl and utilization |
| CN101081748A (en) * | 2006-05-29 | 2007-12-05 | 天津市汉沽区福祥肥料加工厂 | Animal dung urine handling method |
| CN105272472A (en) * | 2015-11-06 | 2016-01-27 | 海南大学 | Biogas slurry formula fertilizer through fermentation of primary pig manure and urine and preparation method thereof |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110156509A (en) * | 2019-06-27 | 2019-08-23 | 浙江亲水园生物科技有限公司 | A kind of in-situ preparation method of urine organic liquor extensive use of chemical fertilizer |
| CN110156509B (en) * | 2019-06-27 | 2021-09-14 | 浙江亲水园生物科技有限公司 | In-situ preparation method for urine organic liquid fertilization |
| CN110937929A (en) * | 2019-12-17 | 2020-03-31 | 江苏豹邦科技发展有限公司 | Anhydrous ecological toilet urine treatment microbial preparation and urine treatment process thereof |
| CN110963834A (en) * | 2019-12-30 | 2020-04-07 | 浙江亲水园生物科技有限公司 | Method for organic liquid fertilization of urine |
| CN110963834B (en) * | 2019-12-30 | 2022-04-19 | 浙江亲水园生物科技有限公司 | Method for organic liquid fertilization of urine |
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