CN109907006A - A method of saving aphidius gifuensis product - Google Patents
A method of saving aphidius gifuensis product Download PDFInfo
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- CN109907006A CN109907006A CN201910226002.5A CN201910226002A CN109907006A CN 109907006 A CN109907006 A CN 109907006A CN 201910226002 A CN201910226002 A CN 201910226002A CN 109907006 A CN109907006 A CN 109907006A
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- aphidius gifuensis
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- saving
- stiff aphid
- matrix
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Abstract
The invention discloses a kind of methods for saving aphidius gifuensis product, comprising the following steps: (1) collects the stiff aphid of the consistent aphidius gifuensis of development duration；(2) matrix, stiff aphid, water are mixed in proportion；(3) the stiff aphid being uniformly mixed with matrix, water is put into freezer and is saved.The present invention realizes the long-acting preservation of aphidius gifuensis product, reduces because of product loss caused by saving, reduces product cost.
Description
Technical field
The present invention relates to technical field of biological control more particularly to a kind of methods for saving aphidius gifuensis product.
Background technique
Cigarette aphid (Myzus persicae) belongs to worldwide pest, and feeding habits are miscellaneous, in addition to tobacco of causing harm, also causes harm other
170 various crops.Cigarette aphid has tender property, and multiple aggregation is caused harm in the tender leaf back side, piercing and sucking juice, makes leaf curl, deformation, interior
Inclusion is reduced, cigarette strain slow growth；" honeydew " of aphid secretion often will lead to tobacco leaf and induce " dark mildew ", become tobacco leaf surface
Black, baking rear blade is brown, low quality, and is difficult to get damp again, and easily broken, shortage gloss causes quality of tobacco to decline.
Causing harm indirectly is to propagate a variety of virus diseases such as tobacco cucumber mosaic virus disease, and alatae is the main media propagated.
Most of in existing technology to be killed by chemical pesticide to cigarette aphid, the use of a large amount of chemical pesticides is not only
Environment is polluted, and causes food safety hazards.In China, aphidius gifuensis is a kind of advantage parasitic wasp of cigarette aphid, individual is small,
Power of circling in the air is strong, parasitic rate is high, is easy for workers to captive breeding, so, biological control is carried out to cigarette aphid using aphidius gifuensis, can be reduced
Persticide residue in tobacco leaf is effectively reduced in Pesticide use amount, reduces influence of the pesticide to environment, improves the safety that tobacco leaf uses
Property, conservation of nature environment.To cigarette aphid in a manner of China's cigarette district has carried out release aphidius gifuensis (mainly in the form of stiff aphid) at present
Biological control is carried out, control efficiency is significant.
Our team are dedicated to the commercialization research of aphidius gifuensis, to improve efficiency, reduce cost as objective, from cigarette aphid cocoon
Bee breeding, automatic processing, extension shelf life, product packaging etc. are studied, and the present invention relies on Yunnan Province tobacco
The provincial science and technology item of company explores the commercialization and industrial application of aphidius gifuensis, solves aphidius gifuensis product
Every technological difficulties, realize aphid parasite product the factorial production in production link.In order to enable aphidius gifuensis product can be big
Area application moves towards multi-form agriculture environment from tobacco field, and this requires can save after aphidius gifuensis production, supply for a long time at any time
Goods.It is in laboratory condition mostly about the store method of aphidius gifuensis in the prior art, operation requires and practical mass production
It disconnects, the preservation effect of aphidius gifuensis is also not ideal enough.Based on this, our team are on the Research foundation of forefathers, in conjunction with us
New discovery during aphidius gifuensis commercialization proposes a kind of preservation cigarette that is easy, inexpensive, meeting mass production environment
The method of aphid parasite product.
Summary of the invention
The purpose of the present invention is to provide a kind of methods for saving aphidius gifuensis product, realize the long-acting of aphidius gifuensis product
It saves, Shelf-life.
To achieve the above object, the following technical solutions are proposed by the present invention:
A method of saving aphidius gifuensis product, which comprises the following steps:
(1) the stiff aphid of the consistent aphidius gifuensis of development duration is collected；
(2) matrix, stiff aphid, water are mixed in proportion；
(3) the stiff aphid being uniformly mixed with matrix, water is put into freezer and is saved；
Preferably, the stiff aphid for educating the phase of going through consistent aphidius gifuensis is the stiff aphid ossify 2-3 days；
Preferably, described that matrix, stiff aphid, water are mixed in proportion and comprised the steps of:
A) matrix, stiff aphid, water are weighed respectively by weight the ratio of 8-10:2.5-3:1-2；
B) matrix and water ratio are uniformly mixed, as material A；
C) it is sealed after mixing in proportion material A with stiff aphid.
Preferably, the matrix is buckwheat shell；
Preferably, the temperature in the freezer changes between 2 DEG C -4 DEG C, keeps dark in freezer；It is further preferred that institute
The temperature stated in freezer is raised to 4 DEG C from 2 DEG C, then the mode for dropping to 2 DEG C from 4 DEG C recycles；It is particularly preferred that being raised to 4 DEG C of consumptions from 2 DEG C
When 7-8 hour, 2 DEG C of time-consuming 7-8 hours are dropped to from 4 DEG C.
The beneficial effects of the present invention are:
(1) store method of the invention is easy, low in cost, can be with aphidius gifuensis production seamless connection.
(2) store method of the invention extends the holding time of aphidius gifuensis product, under the same holding time, cigarette
The eclosion rate of aphid parasite is significantly improved, and has unexpected technical effect.Reduce loss caused by preservation, drop simultaneously
Low product cost.
Detailed description of the invention
Present invention will be further explained below with reference to the attached drawings and examples；
Fig. 1 is the stiff aphid product emergence lab diagram of the preferred embodiment of the present invention.
Specific embodiment
Clear, complete description is carried out to the technical solution of the embodiment of the present invention below:
Embodiment 1
Test place: Kunming Xun Dian tobacco technology centre for spreading
Experimental method:
1, the stiff aphid 2.5g (5082 stiff aphids) that acquisition ossifys 2-3 days；
2, it after mixing by 10g buckwheat shell and 2g water, adds 2.5g deadlock aphid to be uniformly mixed, being then charged into diameter is
Closed preservation in the round plastic bottle of 8.5cm；
3, above-mentioned round plastic bottle being put into freezer, keeps dark in freezer, the temperature in freezer is raised to 4 DEG C from 2 DEG C,
2 DEG C are dropped to from 4 DEG C again, is so recycled；It is raised to 4 DEG C of time-consuming 7-8 hours from 2 DEG C, 2 DEG C of time-consuming 7-8 hours are dropped to from 4 DEG C；
4,2 processing: refrigeration 25 days (processing I), 30 days (handling II) are set.Round plastic bottle is taken out after refrigeration, is opened
Plastic bottle closure lives plastic bottle opening with the fly net pocket cover of 80 mesh, then plastic bottle is put into growth cabinet, allows stiff aphid plumage
Change, artificial climate the temperature inside the box is 25 DEG C, relative humidity 80%.Statistics aphidius gifuensis eclosion rate daily.Each processing repeats 3
It is secondary.
Control group A:
1, the stiff aphid 2.5g (5016 stiff aphids) that acquisition ossifys 2-3 days；
2, it after mixing by 10g buckwheat shell and 2g water, adds 2.5g deadlock aphid to be uniformly mixed, being then charged into diameter is
Closed preservation in the round plastic bottle of 8.5cm；
3, above-mentioned round plastic bottle is put into freezer, keeps dark in freezer, the temperature in freezer is 3 DEG C；
4,2 processing: refrigeration 25 days (processing α), 30 days (handling β) are set.Round plastic bottle is taken out after refrigeration, opens modeling
Expect bottle cap, live plastic bottle opening with the fly net pocket cover of 80 mesh, then plastic bottle is put into growth cabinet, stiff aphid is allowed to sprout wings,
Artificial climate the temperature inside the box is 25 DEG C, relative humidity 80%.Statistics aphidius gifuensis eclosion rate daily.Each processing is repeated 3 times.
Control group B:
1, the stiff aphid 2.5g (5061 stiff aphids) that acquisition ossifys 2-3 days；
2,2.5g deadlock aphid is fitted into closed preservation in the round plastic bottle that diameter is 8.5cm；
3, above-mentioned round plastic bottle being put into freezer, keeps dark in freezer, the temperature in freezer is raised to 4 DEG C from 2 DEG C,
The mode for dropping to 2 DEG C from 4 DEG C again recycles；It is raised to 4 DEG C of time-consuming 7-8 hours from 2 DEG C, 2 DEG C of time-consuming 7-8 hours are dropped to from 4 DEG C；
4,2 processing: refrigeration 25 days (processing III), 30 days (processing IV) are set.Round plastic bottle is taken out after refrigeration, is opened
Plastic bottle closure lives plastic bottle opening with the fly net pocket cover of 80 mesh, then plastic bottle is put into growth cabinet, allows stiff aphid plumage
Change, artificial climate the temperature inside the box is 25 DEG C, relative humidity 80%.Statistics aphidius gifuensis eclosion rate daily.Each processing repeats 3
It is secondary.
Eclosion rate after the refrigeration of 1 aphidius gifuensis product of table
| Processing | Cold preservation time | Eclosion rate |
| Handle I | 25 days | 87.91% |
| Handle II | 30 days | 81.25% |
| Handle α | 25 days | 73.69% |
| Handle β | 30 days | 58.72% |
| Processing III | 25 days | 51.92% |
| Processing IV | 30 days | 41.33% |
As shown in Table 1, the eclosion rate after the present invention saves aphidius gifuensis product 25 days, 30 days is significantly higher than the prior art
In, reduce product because of loss caused by saving, reduces costs.
Based on the above description of the preferred embodiments of the present invention, through the above description, related personnel completely can be with
Without departing from the scope of the technological thought of the present invention', various changes and amendments are carried out.The technical scope of this invention
It is not limited to the contents of the specification, it is necessary to determine the technical scope according to the scope of the claims.
Claims (7)
1. a kind of method for saving aphidius gifuensis product, which comprises the following steps:
(1) the stiff aphid of the consistent aphidius gifuensis of development duration is collected；
(2) matrix, stiff aphid, water are mixed in proportion；
(3) the stiff aphid being uniformly mixed with matrix, water is put into freezer and is saved.
2. the method according to claim 1 for saving aphidius gifuensis product, which is characterized in that the development duration is consistent
The stiff aphid of aphidius gifuensis is the stiff aphid ossify 2-3 days.
3. the method according to claim 1 for saving aphidius gifuensis product, which is characterized in that described by matrix, stiff aphid, water
It mixes in proportion and comprises the steps of:
A) matrix, stiff aphid, water are weighed respectively by weight the ratio of 8-10:2.5-3:1-2；
B) matrix and water ratio are uniformly mixed, as material A；
C) it is sealed after mixing in proportion material A with stiff aphid.
4. the method according to claim 3 for saving aphidius gifuensis product, which is characterized in that the matrix is buckwheat shell.
5. the method according to claim 1 for saving aphidius gifuensis product, which is characterized in that the temperature in the freezer exists
Change between 2 DEG C -4 DEG C, keeps dark in freezer.
6. the method according to claim 1 for saving aphidius gifuensis product, which is characterized in that temperature in the freezer from
2 DEG C are raised to 4 DEG C, then the mode for dropping to 2 DEG C from 4 DEG C recycles.
7. the method according to claim 6 for saving aphidius gifuensis product, which is characterized in that be raised to 4 DEG C of time-consuming 7- from 2 DEG C
8 hours drop to 2 DEG C of time-consuming 7-8 hours from 4 DEG C.
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| CN201910226002.5A CN109907006B (en) | 2019-03-25 | 2019-03-25 | Method for preserving aphidius gifuensis ashmead products |
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| CN201910226002.5A CN109907006B (en) | 2019-03-25 | 2019-03-25 | Method for preserving aphidius gifuensis ashmead products |
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| CN109907006A true CN109907006A (en) | 2019-06-21 |
| CN109907006B CN109907006B (en) | 2021-07-20 |
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| CN104585135A (en) * | 2014-12-21 | 2015-05-06 | 辽宁省烟草公司朝阳市公司 | Overwintering breeding method for aphidiusgifuensis in northern area |
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| CN101003320A (en) * | 2006-01-19 | 2007-07-25 | 中国水产科学研究院东海水产研究所 | Method of packing offspring of blue crab for transport |
| CN101455190A (en) * | 2008-12-30 | 2009-06-17 | 云南省烟草公司玉溪市公司 | Aphidius gifuensis Ashmaed scale propagation technique |
| CN104585135A (en) * | 2014-12-21 | 2015-05-06 | 辽宁省烟草公司朝阳市公司 | Overwintering breeding method for aphidiusgifuensis in northern area |
| CN104642267B (en) * | 2015-02-09 | 2018-05-04 | 云南绿叶生防科技有限公司 | A kind of industrialization breeding method of aphidius gifuensis |
| CN205671285U (en) * | 2016-06-11 | 2016-11-09 | 贵州大学 | Stiff aphid breeding apparatus |
| CN108207822A (en) * | 2018-03-12 | 2018-06-29 | 内蒙古农业大学 | A kind of artificial a large amount of fast breeding technical methods of brown band aphid chalcid fly |
| CN109122672A (en) * | 2018-07-18 | 2019-01-04 | 安徽德昌苗木有限公司 | A kind of storage method of oil tea breeding fringe bar heat preserving antistaling |
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| 阙劲松等: "烟蚜茧蜂产品生产及应用技术研究（Ⅲ）——僵蚜产品低温冷藏技术", 《南方农业学报》 * |
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| CN109907006B (en) | 2021-07-20 |
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Effective date of registration: 20210816 Address after: 650051 No. 523, Beijing Road, Kunming, Yunnan Patentee after: KUNMING COMPANY, YUNNAN TOBACCO Co. Patentee after: Yunnan promoter Biotechnology Co.,Ltd. Address before: 650051 No. 523, Beijing Road, Kunming, Yunnan Patentee before: KUNMING COMPANY, YUNNAN TOBACCO Co. Patentee before: YUNNAN HENGCAO BIOTECHNOLOGY Co.,Ltd. |
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