CN105831017B - A kind of receipts ovum method of chrysomyia megacephala larva and application - Google Patents
A kind of receipts ovum method of chrysomyia megacephala larva and application Download PDFInfo
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- CN105831017B CN105831017B CN201610194635.9A CN201610194635A CN105831017B CN 105831017 B CN105831017 B CN 105831017B CN 201610194635 A CN201610194635 A CN 201610194635A CN 105831017 B CN105831017 B CN 105831017B
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- ovum
- matrix
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- receipts
- egg
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- 102000002322 Egg Proteins Human genes 0.000 title claims abstract description 91
- 108010000912 Egg Proteins Proteins 0.000 title claims abstract description 91
- 210000004681 ovum Anatomy 0.000 title claims abstract description 91
- 241000202815 Chrysomya megacephala Species 0.000 title claims abstract description 40
- 238000000034 method Methods 0.000 title claims abstract description 22
- 239000011159 matrix material Substances 0.000 claims abstract description 45
- 210000003608 fece Anatomy 0.000 claims abstract description 36
- 239000010871 livestock manure Substances 0.000 claims abstract description 36
- 238000000855 fermentation Methods 0.000 claims abstract description 18
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 18
- 230000012447 hatching Effects 0.000 claims abstract description 16
- 235000015099 wheat brans Nutrition 0.000 claims abstract description 14
- 230000003020 moisturizing effect Effects 0.000 claims abstract description 8
- 239000000463 material Substances 0.000 claims abstract description 7
- 230000029142 excretion Effects 0.000 claims abstract description 4
- 230000017448 oviposition Effects 0.000 claims description 9
- 238000001035 drying Methods 0.000 claims description 4
- 239000007921 spray Substances 0.000 claims description 4
- 230000003115 biocidal effect Effects 0.000 claims description 3
- 238000009395 breeding Methods 0.000 claims description 3
- 230000001488 breeding effect Effects 0.000 claims description 3
- 239000003814 drug Substances 0.000 claims description 3
- 238000002360 preparation method Methods 0.000 claims description 3
- 238000004321 preservation Methods 0.000 claims description 3
- 239000002352 surface water Substances 0.000 claims description 3
- 239000002985 plastic film Substances 0.000 claims 1
- 229920006255 plastic film Polymers 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 19
- 230000004083 survival effect Effects 0.000 abstract description 3
- 241000238631 Hexapoda Species 0.000 abstract description 2
- 238000007796 conventional method Methods 0.000 abstract 1
- 230000008719 thickening Effects 0.000 abstract 1
- 230000004151 fermentation Effects 0.000 description 8
- 238000012545 processing Methods 0.000 description 8
- 238000010835 comparative analysis Methods 0.000 description 7
- 238000012360 testing method Methods 0.000 description 6
- 239000005996 Blood meal Substances 0.000 description 5
- 238000002474 experimental method Methods 0.000 description 4
- 241000257159 Musca domestica Species 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 230000000052 comparative effect Effects 0.000 description 2
- 239000010408 film Substances 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 238000005457 optimization Methods 0.000 description 2
- 239000004033 plastic Substances 0.000 description 2
- 229920003023 plastic Polymers 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 239000010409 thin film Substances 0.000 description 2
- 210000002700 urine Anatomy 0.000 description 2
- 208000001034 Frostbite Diseases 0.000 description 1
- 238000007605 air drying Methods 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 210000004700 fetal blood Anatomy 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000003895 organic fertilizer Substances 0.000 description 1
- 235000015277 pork Nutrition 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 230000000284 resting effect Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention belongs to propagate insect technique field artificially, a kind of receipts ovum method more particularly to chrysomyia megacephala larva and application.The present invention includes using pig manure as the receipts ovum material of matrix and receiving ovum method, it is characterised in that：(1) pig manure that ovum matrix is selected from same day excretion is received, makes its water content to 75%, 6 12h of anaerobic fermentation must receive ovum matrix；(2) ovum disk quadrangle and edge thickening placement receipts ovum matrix are being received；(3) daily 8 18h is put into fly cage by ovum matrix is received, and a fly blow is received per 2h, and 4 DEG C of the pieces of an egg on the same day are preserved, hatching is concentrated at 30 DEG C；(4) addition in due course receives ovum matrix and matrix is kept to moisten after collecting pieces of an egg；(5) wheat bran that 65% water content is covered on pieces of an egg surface covers moisturizing with film, hatches in 30 DEG C of environment.The present invention solves the problems, such as chrysomyia megacephala while hatching, and receives ovum matrix and is better than conventional method, at low cost, receives that ovum effect is good and the survival rate height of ovum.
Description
Technical field
The invention belongs to propagate insect technique field artificially.It a kind of receipts ovum method more particularly to chrysomyia megacephala larva and answers
With.
Background technology
The discharge capacity of the annual pig manure in China is up to 2.13 hundred million tons, but resource utilization rate is insufficient, at chrysomyia megacephala
Pig manure is managed, not only efficient in 3-5 days and effective solution problem of environmental pollution additionally provides high-quality protein feed and organic
Fertilizer provides new way for recycling pig manure.
In general, the pig manure water content of pig drained naturally is between 68-76%.
Traditionally the receipts ovum matrix of artificial breeding chrysomyia megacephala is mainly the following raw material：It is spoil, aqueous
(wheat bran and blood meal mass ratio are=9 to the mixture of 65% fermentation wheat bran, the fermentation wheat bran of water content 65% and blood meal:1).With
Upper three kinds of modes, first way are that smell is big, preserve materials and have any problem, it is difficult to industrial operation；The second way can agent
Type is factory produced, and more hygienic, cost is relatively low, but it is poor to receive ovum effect；The third mode can be factory produced,
Effect is preferable, but production cost is more expensive.
At present both at home and abroad it is generally acknowledged that chrysomyia megacephala larva processing food garbage effect is good, processing pig manure effect is simultaneously bad,
It is not high to the utilization rate of excrement.
Invention content
It is an object of the invention to overcome the deficiencies of the prior art and provide a kind of receipts ovum method of chrysomyia megacephala larva and answer
With.It solves the problems, such as that the golden fly receipts ovum matrix of existing artificial room's cultivation is of high cost, effect is undesirable and materials are inconvenient.
The present invention is the mostly generation domestication (conventionally) after artificial primary dcreening operation goes out and handles the chrysomyia megacephala of pig manure, selection and breeding
Go out to handle the good chrysomyia megacephala of pig manure effect (the present invention is not limited to the chrysomyia megacephalas of the domestication for implementation), based on this, gropes
Go out to be suitble to the most suitable receipts ovum material formula and mode of the chrysomyia megacephala.
Chrysomyia megacephala larva head is bigger than housefly, and separating effect is also better than housefly, can replace housefly in handling pig manure
Status.
Realize that technical solution of the present invention is as described below：
A kind of receipts ovum method of chrysomyia megacephala larva, including produce using pig manure as matrix and receive ovum material and the receipts ovum after optimization
Method,
It is described that steps are as follows：
(1) preparation of ovum matrix is received：Receive the excrement that ovum matrix is selected from 2-3 monthly age porkling of same day excretion, the excrement
Just it is free of in and kills fly medicament and antibiotic；By drying or adding the pig urine of 2-3 monthly age piglet, make its water content extremely
75%, it is subsequently placed in anaerobic fermentation 6-12h at room temperature, obtains fluffy receipts ovum matrix；
(2) placement of ovum matrix is received：First the wheat bran of water content 65% is laid in and is received in ovum box, thickness reaches 2cm, then will
Ovum matrix recited above of receiving is placed on the quadrangle for receiving ovum box and all edge lines, i.e. chrysomyia megacephala likes the region of oviposition；
(3) ovum and hatching are received in due course：Daily 8-18h is put into fly cage by ovum matrix is received, and receives a fly blow per 2h, collects the same day
Pieces of an egg, 4 DEG C preservation；
(4) moisturizing of ovum matrix is received：After collecting pieces of an egg, addition in due course receives ovum matrix and to ovum stromal surface water spray is received, and protects
Hold the moistening for receiving ovum stromal surface；
(5) pieces of an egg surface covers：Pieces of an egg surface covers the wheat bran of 65% water content of one layer of 0.5cm, is used in combination plastics thin
Film covers moisturizing, and pieces of an egg are placed in 30 DEG C of environment and are hatched.
Compared with prior art, the beneficial effects of the invention are as follows：
(1) present invention improve receive ovum effect, receive ovum matrix than, receive ovum rate be better than traditional method.See Fig. 1.
(2) present invention improves the survival rate that chrysomyia megacephala is incubated, since pig manure is fluffy, and artificial water spray in time, also
Moist keeping measures improve the survival rate of ovum.
(3) it solves the problems, such as chrysomyia megacephala while hatching, due to Cord blood so that the chrysomyia megacephala morning and at dusk production
Ovum is preparing to be put into hatching vessel, when high temperature is hatched, can synchronize out, and place mat has been made for follow-up convenient separation.
(4) present invention improves economic benefit and social benefit, and the present invention receives ovum using pig manure as chrysomyia megacephala larva
Matrix, raw material is easier to obtain than traditional spoil, wheat bran, blood meal etc., thus at low cost.
Description of the drawings
Fig. 1：It is the spawning rate comparative analysis for receiving ovum rate and traditional method implementation result of the present invention.
Fig. 2：It is oviposition effect of the chrysomyia megacephala to different pig age pig manures of the present invention.
Fig. 3：It is spawning rate comparative analysis of the chrysomyia megacephala of the present invention to different pig age pig manures.
Fig. 4：It is oviposition effect of the chrysomyia megacephala to different moisture content pig manure of the present invention.
Fig. 5：It is spawning rate comparative analysis of the chrysomyia megacephala of the present invention to different moisture content pig manure.
Fig. 6：Be the present invention chrysomyia megacephala to pig manure fermentation whether oviposition effect.
Fig. 7：Be the present invention chrysomyia megacephala to pig manure fermentation whether spawning rate comparative analysis.
Fig. 8：It is that the chrysomyia megacephala of the present invention stores pig manure the oviposition effect of number of days.
Fig. 9：It is that the chrysomyia megacephala of the present invention stores pig manure the spawning rate comparative analysis of number of days.
Figure 10：It is oviposition effect of the chrysomyia megacephala to the pig manure fermentation time of the present invention.
Figure 11：It is spawning rate comparative analysis of the chrysomyia megacephala of the present invention to the pig manure fermentation time.
Figure 12：It is the hatching rate comparative analysis of the chrysomyia megacephala larvae hatch ovum at different temperatures of the present invention.
Figure 13：Be the chrysomyia megacephala larvae hatch of the present invention whether film covers in the case of ovum hatching rate compare point
Analysis.
Figure 14：It is the different attraction effects for receiving ovum matrix to egg-laying season chrysomyia megacephala to fermentation of the present invention.
The matrix of reference sign, the upper figure of Figure 14 is pig manure, and the middle figure matrix of Figure 14 is mixture (blood meal and bran
Skin), the matrix of Figure 14 figure below is wheat bran.
Specific implementation mode
Receipts ovum disk, wheat bran, blood meal for being used in the embodiment of the present invention etc. are commercially available, and fresh pig manure is collected by farm.
1 generic embodiment of embodiment
A kind of receipts ovum method of chrysomyia megacephala larva, including produce using pig manure as matrix and receive ovum material and the receipts ovum after optimization
Method specifically includes the following steps
(1) preparation of ovum matrix is received：Receive the excrement that ovum matrix is selected from 2-3 monthly age porkling of same day excretion, the excrement
Just it is free of in and kills fly medicament and antibiotic；By drying or adding the pig urine of 2-3 monthly age piglet, make its water content extremely
75%, it is subsequently placed in anaerobic fermentation 6-12h at room temperature, obtains fluffy receipts ovum matrix；
(2) placement of ovum matrix is received：First the wheat bran of water content 65% is laid in and is received in ovum box, thickness reaches 2cm, then will
Ovum matrix recited above of receiving is placed on the quadrangle for receiving ovum box and all edge lines, i.e. chrysomyia megacephala likes the region of oviposition；
(3) ovum and hatching are received in due course：Daily 8-18h is put into fly cage by ovum matrix is received, and receives a fly blow per 2h, collects the same day
Pieces of an egg, 4 DEG C preservation；
(4) moisturizing of ovum matrix is received：After collecting pieces of an egg, addition in due course receives ovum matrix and to ovum stromal surface water spray is received, and protects
Hold the moistening for receiving ovum stromal surface；
(5) pieces of an egg surface covers：Pieces of an egg surface covers the wheat bran of 65% water content of one layer of 0.5cm, is used in combination plastics thin
Film covers moisturizing, and pieces of an egg are placed in 30 DEG C of environment and are hatched.
2 comparing embodiment of embodiment
In the present embodiment, we compare following several different embodiments：
(1) with reference to the universal method of embodiment 1, relevant comparative test has been carried out to the selection of different pig age pig manures, has been had
Body is：It chooses pork chop and lets out rear 2-3 a month, 4-5 months, 6-7 months, 8-9 months, 10-11 months and the pig manure work at 12 monthly ages
Nature normal temperature anaerobic fermentation processing is carried out to receive ovum matrix, ferment 6h, and receipts ovum is carried out at the end of processing, receives ovum design sketch and sees Fig. 2,
Spawning rate is shown in Fig. 3.
(2) with reference to the universal method of embodiment 1, relevant comparative test has been carried out to the selection of pig manure water content, specifically
It is：It chooses the fresh pig manure that water content is 65%, 75%, 85% and carries out nature normal temperature anaerobic fermentation processing, ferment 6h, processing knot
The receipts ovum matrix that chrysomyia megacephala is obtained when beam obtains receipts ovum effect as described in Figure 4 and production as described in Figure 5 by culture experiment
Ovum rate.
(3) with reference to the universal method of embodiment 1, correlation test has been carried out to pig manure fermentation and azymic：It chooses aqueous
75% pig manure carries out the processing of nature anaerobic fermentation and azymic 12h respectively, and embodiment 1 is led in other operations.Invention effect is shown in
Fig. 6, spawning rate are shown in Fig. 7.
(4) with reference to the universal method of embodiment 1, correlation test has been carried out to the pig manure resting period：Choose the same day i.e. 0 day, 3
It, 5 days pig manures, adjust water content to 75% using drying or natural air drying, then carry out the processing of nature anaerobic fermentation and its
He operates (referring to embodiment 1).Invention effect is shown in that Fig. 8, spawning rate are shown in Fig. 9.
(5) with reference to the universal method of embodiment, correlation test has been carried out to the pig manure nature anaerobic fermentation time：Choose nature
Anaerobic fermentation 0h, 6h, 12h, for 24 hours, other operations are with reference to embodiments 1.Invention effect is shown in that Figure 10, spawning rate are shown in Figure 11.
(6) on the synchronization hatching rate of ovum, low temperature can delay to hatch, with the premise of not frostbite ovum in the selection of low temperature
Under, to ensure the hatching rate of ovum.Low-temperature treatment chooses 4 DEG C, 9 DEG C, 15 DEG C of progress, other operations observe major part with reference to embodiment 1
The hatching rate of golden fly blow.This experiment is shown in Figure 12 to the testing result of hatching rate.
(7) experiment of the surface moist keeping measures of ovum.Choosing pieces of an egg upper surface one layer of (thickness tail 0.5cm) water content of covering is
65% wheat bran simultaneously carries out moisturizing experiment with film covering, is control with no Water-retaining Measure, by the larva of chrysomyia megacephala at 30 DEG C
Lower hatching, observes the hatching rate of ovum.Obtain hatching rate as shown in fig. 13 that.
Claims (2)
1. a kind of receipts ovum method of chrysomyia megacephala larva, including produce using pig manure as the receipts ovum material of matrix and receive ovum method, it is special
Sign is：
(1) preparation of ovum matrix is received：The excrement that ovum matrix is selected from the 2-3 monthly age porklings of same day excretion is received, in the excrement not
Containing killing fly medicament and antibiotic；Pig by drying or adding 2-3 monthly age piglets urinates, and makes its water content to 75%, then sets
Anaerobic fermentation 6-12h at room temperature obtains fluffy receipts ovum matrix；
(2) placement of ovum matrix is received：First the wheat bran of water content 65% is laid in and is received in ovum box, thickness reaches 2cm, then will be above
The receipts ovum matrix is placed on the quadrangle for receiving ovum box and all edge lines, i.e. chrysomyia megacephala likes the region of oviposition；
(3) ovum and hatching are received in due course：Ovum matrix will be received daily and is put into fly cage 8-18h, and a fly blow is received per 2h, collects the ovum on the same day
Block, 4 DEG C of preservations；
(4) moisturizing of ovum matrix is received：After collecting pieces of an egg, addition in due course receives ovum matrix and to ovum stromal surface water spray is received, and keeps receiving
The moistening of ovum stromal surface；
(5) pieces of an egg surface covers：Pieces of an egg surface covers the wheat bran of 65% water content of one layer of 0.5cm, and plastic film is used in combination to cover
Pieces of an egg are placed in 30 DEG C of environment and hatch by lid moisturizing.
2. application of the method described in claim 1 in chrysomyia megacephala artificial breeding.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610194635.9A CN105831017B (en) | 2016-03-31 | 2016-03-31 | A kind of receipts ovum method of chrysomyia megacephala larva and application |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610194635.9A CN105831017B (en) | 2016-03-31 | 2016-03-31 | A kind of receipts ovum method of chrysomyia megacephala larva and application |
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| CN105831017A CN105831017A (en) | 2016-08-10 |
| CN105831017B true CN105831017B (en) | 2018-09-11 |
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| CN109122587B (en) * | 2018-07-21 | 2020-11-24 | 华中农业大学 | Method for breeding harmonia axyridis by using chrysomyia megacephala larvae |
| NL2024692B9 (en) * | 2020-01-17 | 2021-11-29 | Protix Bv | Method for controlling hatching of an insect egg |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101156564A (en) * | 2007-11-13 | 2008-04-09 | 广东省昆虫研究所 | A method for enticing collection of housefly oosphere |
| TW201130412A (en) * | 2010-03-01 | 2011-09-16 | Pro Eco Biotech Internat Co Ltd | Manufacturing method of nutritional substance containing housefly pupae |
| CN102351576A (en) * | 2011-07-07 | 2012-02-15 | 中山大学 | Method of treating horse dung with Chrysomya megacephala to prepare organic fertilizer |
| CN104855340A (en) * | 2015-05-20 | 2015-08-26 | 乐山师范学院 | Phaenicia sericata egg collecting object and fabrication method thereof |
-
2016
- 2016-03-31 CN CN201610194635.9A patent/CN105831017B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101156564A (en) * | 2007-11-13 | 2008-04-09 | 广东省昆虫研究所 | A method for enticing collection of housefly oosphere |
| TW201130412A (en) * | 2010-03-01 | 2011-09-16 | Pro Eco Biotech Internat Co Ltd | Manufacturing method of nutritional substance containing housefly pupae |
| CN102351576A (en) * | 2011-07-07 | 2012-02-15 | 中山大学 | Method of treating horse dung with Chrysomya megacephala to prepare organic fertilizer |
| CN104855340A (en) * | 2015-05-20 | 2015-08-26 | 乐山师范学院 | Phaenicia sericata egg collecting object and fabrication method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 蝇蛆生态处理猪粪对家蝇产卵和孵化的影响;杨鹤萍等;《中国寄生虫学与寄生虫病杂志》;20040229;第22卷(第1期);第9-10页 * |
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