CN109876062A - Facilitate the Chinese medicine powder and the preparation method and application thereof of melanin of black-bone chicken deposition - Google Patents
Facilitate the Chinese medicine powder and the preparation method and application thereof of melanin of black-bone chicken deposition Download PDFInfo
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Abstract
The invention discloses a kind of Chinese medicine powders for facilitating melanin of black-bone chicken deposition, are mainly prepared by the raw material of following parts by weight: 25~30 parts, 25~30 parts of saw-toothed grain beetle, 25~30 parts of pheretima, 15~20 parts of the fruit of glossy privet, 15~20 parts of safron, 7~15 parts of Phoenix Tree Seed, 7~15 parts of rhizome of chuanxiong, 3~6 parts of Radix Salviae Miltiorrhizae, 3~6 parts of Poria cocos, 3~6 parts of seaweed are stolen in 35~40 parts of Eupolyphoge sinensis, 25~30 parts of rice weevil, medicine-valley.The invention discloses a kind of preparation method of Chinese medicine powder, Eupolyphoge sinensis, pheretima, the fruit of glossy privet, safron, Phoenix Tree Seed etc. are crushed, and are mixed to get mixture, using alcohol extracting method extraction mixture.The invention discloses a kind of Chinese medicine powders in the application for improving the Gallus domesticlus brisson cock melanin deposition that reaches the clouds.The present invention has copper, the tyrosine, vitamin B improved in Chinese medicine powder6Content, improve Gallus domesticlus brisson cock body in melanin content beneficial effect.
Description
Technical field
The present invention relates to feed additive fields.It sinks it is more particularly related to which one kind facilitates melanin of black-bone chicken
Long-pending Chinese medicine powder and the preparation method and application thereof.
Background technique
Gallus domesticlus brisson of reaching the clouds is gained the name because originating in Lingyun county of Guangxi, beak, hat, shin toe, skin, meat and internal organ be it is pitch-black, when
The ground masses are commonly called as " five Gallus domesticlus brissons ".The kind has resistance to crude feed, ability of looking for food and feather is beautiful, fine and tender taste, delicious aromatic, food
The features such as high with medical value is delicacies and excellent tonic product on dining table.Black-bone chicken is the important characteristic medicine food resource in China, with
Non- black-bone chicken is compared, and most significant feature is that it is rich in melanin in vivo, and melanin is the weight that black-bone chicken plays its medicinal efficacy
Want material base.For a long time, people are using black-bone chicken as the good merchantable brand of traditional Chinese medicine and nourishing, modern studies have found that, black bone
Chicken dark pigment has antifatigue, anti-oxidant, anti-mutagenesis, radiation protection, anti-aging, improves immunity of organisms, lead discharging removing toxic substances, eliminates
The functions such as free radical, melanin are active constituents important in body, are the material bases of active function, melanin determines crow
The medical value of bone chicken.The higher Gallus domesticlus brisson of internal melanin content, medicinal nourishing value are higher.The storage of melanin in Gallus domesticlus brisson body
Accumulated amount has outside the Pass in addition to the inherent cause with chicken, non-genetic factor, as also to influence Gallus domesticlus brisson black for the factor of feed, disease etc.
The formation of pigment.Meanwhile by repeatedly cultivating discovery, the black degree for Gallus domesticlus brisson cock of reaching the clouds is compared with the hen for Gallus domesticlus brisson of reaching the clouds no matter
It is skin surface or internal organ and bone, black degree is not as good as hen.
Summary of the invention
It is an object of the invention to solve at least the above problems, and provide the advantages of at least will be described later.
It is a still further object of the present invention to provide a kind of Chinese medicine powders for facilitating melanin of black-bone chicken deposition, help to reach the clouds
Gallus domesticlus brisson cock melanin deposition provides a kind of preparation method of Chinese medicine powder for facilitating melanin of black-bone chicken deposition, can be improved
Copper, tyrosine, vitamin B in Chinese medicine powder6Content, a kind of Chinese medicine powder is provided and is reached the clouds Gallus domesticlus brisson cock melanin in raising
The application of deposition.
In order to realize these purposes and other advantages according to the present invention, providing one kind facilitates melanin of black-bone chicken deposition
Chinese medicine powder, mainly by following parts by weight raw material prepare: 35~40 parts of Eupolyphoge sinensis, 25~30 parts of rice weevil, medicine-valley steal 25~30
Part, 25~30 parts of saw-toothed grain beetle, 25~30 parts of pheretima, 15~20 parts of the fruit of glossy privet, 15~20 parts of safron, 7~15 parts of Phoenix Tree Seed, river
7~15 parts of vault, 3~6 parts of Radix Salviae Miltiorrhizae, 3~6 parts of Poria cocos, 3~6 parts of seaweed.
A kind of preparation method of Chinese medicine powder facilitating melanin of black-bone chicken deposition is provided, Eupolyphoge sinensis, rice weevil, medicine-valley are stolen,
Saw-toothed grain beetle, pheretima, the fruit of glossy privet, safron, Phoenix Tree Seed, rhizome of chuanxiong, Radix Salviae Miltiorrhizae, Poria cocos, seaweed crush, and are mixed to get mixture, adopt
With alcohol extracting method extraction mixture, obtained extract is Chinese medicine powder.
Preferably, alcohol extracting method specifically: the ethanol solution that mass fraction is 95% is added into mixture, is soaked under room temperature
2~4h is mentioned, filtrate A and filter residue A is filtered to obtain, filtrate A is evaporated under reduced pressure, and is volatilized and is removed ethyl alcohol and obtain alcohol extract, alcohol extract is dense
It is reduced to the clear cream of relative density 1.15~1.25, then is uniformly mixed with silica with mass ratio 1:3, drying is low to moisture content
In 5%, the Chinese medicine powder is obtained, wherein the mass ratio for the ethanol solution that mixture and mass fraction are 95% is 1:3.
It preferably, further include that freeze thawing treatment, specific method are carried out to mixture before using alcohol extracting method extraction mixture
Are as follows:
In mass ratio it is that 1:1 stirs into paste by mixture and drinking water, paste is placed in -30~-20 DEG C of temperature
Degree is lower to freeze 20~30h, is subsequently placed at a temperature of 20~30 DEG C, until paste thaws completely.
Preferably, the grain diameter of mixture is less than 100 mesh.
Preferably, before being evaporated under reduced pressure filtrate A, further includes: matter is added into filter residue A for 1:3 in mass ratio
The ethanol solution that score is 95% is measured, 2~4h is extracted under room temperature, filters to obtain liquor B and filter residue B, is in mass ratio 1:3 to filter residue
The ethanol solution that mass fraction is 95% is added in B, extracts 2~4h under room temperature, filters to obtain liquor C, liquor B and liquor C are added
Enter into filtrate A.
It preferably, further include being pre-processed to mixture, specific method before carrying out freeze thawing treatment to mixture
Are as follows:
Dissolution liquid is added into mixture for 1:5 in mass ratio, after mixing evenly, positive electrode and negative electrode are inserted into, and
Turn-on current intensity is the electric current of 10mA, energization 50min, is then placed in that supersonic frequency is 80kHz, temperature is ultrasonic at 60 DEG C again
Handle 40min, wherein dissolution liquid specifically: salt, sodium citrate, potassium dihydrogen phosphate, pineapple pulp juice, drinking water are pressed into matter
Amount for 0.1:0.2:0.2:1:2 than being uniformly mixed;
Wherein, pineapple pulp is beaten filtering taking juice to get pineapple pulp juice.
A kind of Chinese medicine powder is provided in the application for improving the Gallus domesticlus brisson cock melanin deposition that reaches the clouds.
The present invention is include at least the following beneficial effects: being used freeze thawing treatment and the three times method of alcohol extracting first, be can be improved
Copper, tyrosine, vitamin B in Chinese medicine powder6Content, and then it is heavy to promote the Chinese medicine powder promotion Gallus domesticlus brisson cock melanin that reaches the clouds
Long-pending effect.Secondly, combining using dissolution liquid with the function of current, the content of the phosphorus and polysaccharide in Chinese medicine powder is improved, in turn
The content of the fat in Gallus domesticlus brisson chicken is reduced, and improves the content of the phosphatide in Gallus domesticlus brisson chicken, the promotion of melanin and phosphatide, rouge
The reduction of fat, the final nutritional quality for promoting Gallus domesticlus brisson.
Further advantage, target and feature of the invention will be partially reflected by the following instructions, and part will also be by this
The research and practice of invention and be understood by the person skilled in the art.
Specific embodiment
The present invention will be further described in detail below with reference to the embodiments, to enable those skilled in the art referring to specification
Text can be implemented accordingly.
It should be noted that experimental method described in following embodiments is unless otherwise specified conventional method, institute
Reagent and material are stated, unless otherwise specified, is commercially obtained.
<embodiment 1>
Facilitate the Chinese medicine powder of melanin of black-bone chicken deposition, is mainly prepared by the raw material of following parts by weight: Eupolyphoge sinensis 35 part, meter
As 25 parts, 25 parts of saw-toothed grain beetle, 25 parts of pheretima, 15 parts of the fruit of glossy privet, 15 parts of safron, 7 parts of Phoenix Tree Seed, rhizome of chuanxiong 7 are stolen in 25 parts, medicine-valley
Part, 3 parts of Radix Salviae Miltiorrhizae, 3 parts of Poria cocos, 3 parts of seaweed.
By Eupolyphoge sinensis, rice weevil, medicine-valley robber, saw-toothed grain beetle, pheretima, the fruit of glossy privet, safron, Phoenix Tree Seed, rhizome of chuanxiong, Radix Salviae Miltiorrhizae, Poria cocos, sea
Algae crushes, and is mixed to get mixture, and using alcohol extracting method extraction mixture, obtained extract is Chinese medicine powder.
Alcohol extracting method is specially that the ethanol solution that mass fraction is 95% is added into mixture, extracts 3h under room temperature, filters,
Filtrate is taken to be drying to obtain Chinese medicine powder, wherein the mass ratio for the ethanol solution that mixture and mass fraction are 95% is 1:3.
<embodiment 2>
Facilitate the Chinese medicine powder of melanin of black-bone chicken deposition, is mainly prepared by the raw material of following parts by weight: Eupolyphoge sinensis 40 part, meter
As 30 parts, 30 parts of saw-toothed grain beetle, 30 parts of pheretima, 20 parts of the fruit of glossy privet, 20 parts of safron, 15 parts of Phoenix Tree Seed, rhizome of chuanxiong 15 are stolen in 30 parts, medicine-valley
Part, 6 parts of Radix Salviae Miltiorrhizae, 6 parts of Poria cocos, 6 parts of seaweed.
By Eupolyphoge sinensis, rice weevil, medicine-valley robber, saw-toothed grain beetle, pheretima, the fruit of glossy privet, safron, Phoenix Tree Seed, rhizome of chuanxiong, Radix Salviae Miltiorrhizae, Poria cocos, sea
Algae crushes, and is mixed to get mixture, and using alcohol extracting method extraction mixture, obtained extract is Chinese medicine powder.
Alcohol extracting method is specially that the ethanol solution that mass fraction is 95% is added into mixture, extracts 3h under room temperature, filters,
Filtrate is taken to be drying to obtain Chinese medicine powder, wherein the mass ratio for the ethanol solution that mixture and mass fraction are 95% is 1:3.
<embodiment 3>
Facilitate the Chinese medicine powder of melanin of black-bone chicken deposition, is mainly prepared by the raw material of following parts by weight: Eupolyphoge sinensis 38 part, meter
As 28 parts, 27 parts of saw-toothed grain beetle, 27 parts of pheretima, 18 parts of the fruit of glossy privet, 17 parts of safron, 10 parts of Phoenix Tree Seed, rhizome of chuanxiong 10 are stolen in 28 parts, medicine-valley
Part, 4 parts of Radix Salviae Miltiorrhizae, 5 parts of Poria cocos, 4 parts of seaweed.
By Eupolyphoge sinensis, rice weevil, medicine-valley robber, saw-toothed grain beetle, pheretima, the fruit of glossy privet, safron, Phoenix Tree Seed, rhizome of chuanxiong, Radix Salviae Miltiorrhizae, Poria cocos, sea
Algae crushes, and is mixed to get mixture, and using alcohol extracting method extraction mixture, obtained extract is Chinese medicine powder.
Alcohol extracting method is specially that the ethanol solution that mass fraction is 95% is added into mixture, extracts 3h under room temperature, filters,
Filtrate is taken to be drying to obtain Chinese medicine powder, wherein the mass ratio for the ethanol solution that mixture and mass fraction are 95% is 1:3.
<embodiment 4>
Facilitate the Chinese medicine powder of melanin of black-bone chicken deposition, is mainly prepared by the raw material of following parts by weight: Eupolyphoge sinensis 38 part, meter
As 28 parts, 27 parts of saw-toothed grain beetle, 27 parts of pheretima, 18 parts of the fruit of glossy privet, 17 parts of safron, 10 parts of Phoenix Tree Seed, rhizome of chuanxiong 10 are stolen in 28 parts, medicine-valley
Part, 4 parts of Radix Salviae Miltiorrhizae, 5 parts of Poria cocos, 4 parts of seaweed.
By Eupolyphoge sinensis, rice weevil, medicine-valley robber, saw-toothed grain beetle, pheretima, the fruit of glossy privet, safron, Phoenix Tree Seed, rhizome of chuanxiong, Radix Salviae Miltiorrhizae, Poria cocos, sea
Algae crushes, and is mixed to get mixture, and using alcohol extracting method extraction mixture, obtained extract is Chinese medicine powder.
Alcohol extracting method specifically: the ethanol solution that mass fraction is 95% is added into mixture, extracts 3h under room temperature, filters
Filtrate A and filter residue A, filtrate A is evaporated under reduced pressure, and volatilize and remove ethyl alcohol and obtain alcohol extract, alcohol extract is concentrated into relative density
1.20 clear cream, then be uniformly mixed with silica with mass ratio 1:3, it is dry to be lower than 5% to moisture content, obtain the traditional Chinese medicine powder
Agent, wherein the mass ratio for the ethanol solution that mixture and mass fraction are 95% is 1:3.
It further include that freeze thawing treatment is carried out to mixture before using alcohol extracting method extraction mixture, method particularly includes:
It is in mass ratio that 1:1 stirs into paste by mixture and drinking water, it is cold at a temperature of paste is placed in -25 DEG C
Freeze 25h, be subsequently placed at a temperature of 25 DEG C, until paste thaws completely.
The grain diameter of mixture is less than 100 mesh.
Before filtrate A is evaporated under reduced pressure, further includes: mass fraction is added into filter residue A for 1:3 in mass ratio is
95% ethanol solution extracts 3h under room temperature, filters to obtain liquor B and filter residue B, quality is added into filter residue B for 1:3 in mass ratio
The ethanol solution that score is 95% extracts 3h under room temperature, filters to obtain liquor C, liquor B and liquor C are added in filtrate A.
<embodiment 5>
Preparation method is the same as embodiment 4, wherein further includes carrying out before carrying out freeze thawing treatment to mixture to mixture
Pretreatment, method particularly includes:
Dissolution liquid is added into mixture for 1:5 in mass ratio, after mixing evenly, positive electrode and negative electrode are inserted into, and
Turn-on current intensity is the electric current of 10mA, energization 50min, is then placed in that supersonic frequency is 80kHz, temperature is ultrasonic at 60 DEG C again
Handle 40min, wherein dissolution liquid specifically: salt, sodium citrate, potassium dihydrogen phosphate, pineapple pulp juice, drinking water are pressed into matter
Amount for 0.1:0.2:0.2:1:2 than being uniformly mixed;
Wherein, pineapple pulp is beaten filtering taking juice to get pineapple pulp juice.
<comparative example 1>
Preparation method is the same as embodiment 3, wherein uses water extraction extraction mixture.Water extraction specifically: by mixture and drink
In mass ratio it is 1:10 mixing with water, and extracts 5h at 65 DEG C, filters, filtrate is taken to be drying to obtain Chinese medicine powder.
<comparative example 2>
Preparation method is the same as embodiment 3, wherein uses multistage microwave amplifier mixture.Microwave loss mechanisms specifically: by mixture
In mass ratio it is that 1:10 is mixed with drinking water, is placed in micro-wave oven, Yu great Huo microwave treatment 5min, filter, takes filtrate dry
Up to Chinese medicine powder.
<comparative example 3>
Preparation method is the same as embodiment 3, wherein the formula of Chinese medicine powder is different, and the formula of Chinese medicine powder is Eupolyphoge sinensis 38 part, meter
As 28 parts, 27 parts of saw-toothed grain beetle, 27 parts of pheretima, 18 parts of the fruit of glossy privet, 17 parts of safron, 10 parts of Phoenix Tree Seed are stolen in 28 parts, medicine-valley.
<comparative example 4>
Preparation method is the same as embodiment 4, wherein does not carry out freeze thawing treatment to mixture, liquor B is not added into filtrate A yet
And liquor C.
<comparative example 5>
Preparation method is the same as embodiment 4, wherein does not carry out freeze thawing treatment to mixture.
<comparative example 6>
Preparation method is the same as embodiment 5, wherein and pretreated method is different, specifically: it is in mass ratio 1:5 to mixture
Middle addition pineapple pulp juice, wherein pineapple pulp is beaten filtering taking juice to get pineapple pulp juice.
<extracting method evaluation test>
1, copper, the tyrosine, vitamin in Chinese medicine powder prepared by embodiment 3, comparative example 1, the method for comparative example 2 are detected
B6Content, embodiment 3 be alcohol extracting method, comparative example 1 be water extraction, comparative example 2 be microwave loss mechanisms, as a result as shown in the table:
Table 1
As seen from the above table, the copper in the Chinese medicine powder of alcohol extracting method extraction, tyrosine, vitamin B6Content be significantly higher than water
The content of tie element in the Chinese medicine powder of formulation and microwave loss mechanisms illustrates that copper, tyrosine, dimension life can be improved in alcohol extracting method method
Plain B6Content.
Detect copper, the tyrosine, vitamin B in Chinese medicine powder prepared by embodiment 4, comparative example 4, the method for comparative example 56
Content, as a result as shown in the table:
Table 2
Group | Copper % | Tyrosine % | Vitamin B6% |
Embodiment 4 | 7.28 | 0.25 | 8.76 |
Comparative example 4 | 4.24 | 0.23 | 5.89 |
Comparative example 5 | 4.62 | 0.28 | 6.73 |
As seen from the above table, the copper in the Chinese medicine powder of the method preparation of embodiment 4, tyrosine, vitamin B6Content it is aobvious
The content for writing tie element in the Chinese medicine powder for the method preparation for being higher than comparative example 4 and comparative example 5 illustrates freeze thawing treatment and three times
The method of alcohol extracting can improve copper, tyrosine, vitamin B6Content.
2, the content of the phosphorus and polysaccharide in Chinese medicine powder prepared by embodiment 4, embodiment 5, the method for comparative example 6 is detected,
As a result as shown in the table:
Table 3
Group | Phosphorus % | Polysaccharide % |
Embodiment 4 | 53.85 | 36.8 |
Embodiment 5 | 67.22 | 40.18 |
Comparative example 6 | 54.29 | 36.12 |
As seen from the above table, compared with embodiment 4, phosphorus and polysaccharide in the Chinese medicine powder of the method for embodiment 5 preparation contain
Amount significantly improves;
Compared with comparative example 6, pineapple pulp juice is only added, is not added with and promotes liquid and carry out the function of current simultaneously, to raising
The content effect of phosphorus and polysaccharide in Chinese medicine powder is unobvious, only dissolution liquid and combine the function of current after, can just get
Significant effect.
<influence of the Chinese medicine powder to Gallus domesticlus brisson quality>
On January 9th, 2018, Rui Dong Farming Ltd., Lingyun county of Guangxi reach the clouds Gallus domesticlus brisson conservation field, choose the icepro for just going out shell
8000 progress feeding experiments of cloud Gallus domesticlus brisson public affairs seedling, chicken group are randomly divided into 8 groups, and every group 1000, control group fed Basic drawing is real
Apply the corresponding method preparation of example 1~5, comparative example 3, the comparative example 6 whole feeding Basic drawing+1000mg/kg during brooding
Chinese medicine powder, raise to 150 ages in days, each group randomly selects 20 Gallus domesticlus brisson cocks of reaching the clouds respectively, detects liver, kidney, pigeon breast
Melanin accumulation in meat and skin detects fat and content of phospholipid in chicken, as a result as shown in table 4 and table 5.
Melanin content measuring method: accurately weigh 10mg tissue sample and be added to 1mL by soluene-350: water is by body
Product is than after 100 DEG C of heating water bath dissolutions, being immediately placed in spectrophotometer under the conditions of measuring 500nm in solution made of 9:1
Absorbance value (A500), the melanin content of tissue sample is compared according to absorbance value.
Table 4
Group | Liver | Kidney | Fresh Grade Breast | Skin |
Control group | 0.108 | 0.102 | 0.089 | 0.147 |
Embodiment 1 | 0.135 | 0.123 | 0.115 | 0.170 |
Embodiment 2 | 0.136 | 0.128 | 0.120 | 0.178 |
Embodiment 3 | 0.139 | 0.125 | 0.118 | 0.179 |
Embodiment 4 | 0.147 | 0.135 | 0.130 | 0.189 |
Embodiment 5 | 0.154 | 0.141 | 0.134 | 0.195 |
Comparative example 3 | 0.121 | 0.109 | 0.097 | 0.168 |
Comparative example 6 | 0.142 | 0.131 | 0.120 | 0.182 |
As seen from the above table, compared with the control group it is found that Examples 1 to 5, comparative example 3, the method for comparative example 6 preparation in
Medicine powder help to improve the liver of Gallus domesticlus brisson cock, kidney, Fresh Grade Breast, skin melanin content, illustrate Examples 1 to 5
The Chinese medicine powder of method preparation can assist melanin deposition in cock body, but the significant effect of comparative example 3 is not as good as implementing
The effect of the Chinese medicine powder of the method preparation of example 3.
Table 5
Group | Fatty % | Phosphatide % |
Embodiment 3 | 3.58 | 5.42 |
Embodiment 6 | 3.06 | 5.68 |
Comparative example 1 | 3.44 | 5.45 |
As seen from the above table, after the Chinese medicine powder for feeding embodiment 5, the content of the fat in Gallus domesticlus brisson chicken can be reduced, and
And the content of phosphatide can also be improved, the medical value of Gallus domesticlus brisson can be improved;
By embodiment 4 it is found that only adding pineapple pulp juice, being not added with promotion liquid and carrying out electricity simultaneously compared with comparative example 6
Stream effect, Chinese medicine powder obtained are unobvious to the effect of the content of the fat reduced in Gallus domesticlus brisson chicken, nor can improve
After the content of phosphatide in Gallus domesticlus brisson chicken, only dissolution liquid and the combination function of current, Chinese medicine powder obtained, to the two ability
Get significant effect.
Although the embodiments of the present invention have been disclosed as above, but its is not only in the description and the implementation listed
With it can be fully applied to various fields suitable for the present invention, for those skilled in the art, can be easily
Realize other modification, therefore without departing from the general concept defined in the claims and the equivalent scope, the present invention is simultaneously unlimited
In specific details and embodiment shown and described herein.
Claims (8)
1. facilitating the Chinese medicine powder of melanin of black-bone chicken deposition, which is characterized in that mainly prepared by the raw material of following parts by weight: soil
Member 35~40 parts, 25~30 parts of rice weevil, medicine-valley steal 25~30 parts, 25~30 parts of saw-toothed grain beetle, 25~30 parts of pheretima, the fruit of glossy privet 15~
20 parts, 15~20 parts of safron, 7~15 parts of Phoenix Tree Seed, 7~15 parts of rhizome of chuanxiong, 3~6 parts of Radix Salviae Miltiorrhizae, 3~6 parts of Poria cocos, seaweed 3~6
Part.
2. facilitating the preparation method of the Chinese medicine powder of melanin of black-bone chicken deposition as described in claim 1, which is characterized in that will
Eupolyphoge sinensis, rice weevil, medicine-valley robber, saw-toothed grain beetle, pheretima, the fruit of glossy privet, safron, Phoenix Tree Seed, rhizome of chuanxiong, Radix Salviae Miltiorrhizae, Poria cocos, seaweed crush, and
It is mixed to get mixture, using alcohol extracting method extraction mixture, obtained extract is Chinese medicine powder.
3. facilitating the preparation method of the Chinese medicine powder of melanin of black-bone chicken deposition as claimed in claim 2, which is characterized in that alcohol
Formulation specifically: the ethanol solution that mass fraction is 95% is added into mixture, extracts 2~4h under room temperature, filters to obtain filtrate A
With filter residue A, filtrate A is evaporated under reduced pressure, and is volatilized and is removed ethyl alcohol and obtain alcohol extract, by alcohol extract be concentrated into relative density 1.15~
1.25 clear cream, then be uniformly mixed with silica with mass ratio 1:3, it is dry to be lower than 5% to moisture content, obtain the traditional Chinese medicine powder
Agent, wherein the mass ratio for the ethanol solution that mixture and mass fraction are 95% is 1:3.
4. facilitating the preparation method of the Chinese medicine powder of melanin of black-bone chicken deposition as claimed in claim 2, which is characterized in that also
It is included in using before alcohol extracting method extraction mixture, freeze thawing treatment is carried out to mixture, method particularly includes:
In mass ratio it is that 1:1 stirs into paste by mixture and drinking water, paste is placed at a temperature of -30~-20 DEG C
20~30h is freezed, is subsequently placed at a temperature of 20~30 DEG C, until paste thaws completely.
5. facilitating the preparation method of the Chinese medicine powder of melanin of black-bone chicken deposition as claimed in claim 2, which is characterized in that mixed
The grain diameter of object is closed less than 100 mesh.
6. facilitating the preparation method of the Chinese medicine powder of melanin of black-bone chicken deposition as claimed in claim 4, which is characterized in that
Before filtrate A is evaporated under reduced pressure, further includes: it is molten that the ethyl alcohol that mass fraction is 95% is added into filter residue A for 1:3 in mass ratio
Liquid extracts 2~4h under room temperature, filters to obtain liquor B and filter residue B, mass fraction is added into filter residue B for 1:3 in mass ratio is
95% ethanol solution extracts 2~4h under room temperature, filters to obtain liquor C, liquor B and liquor C are added in filtrate A.
7. facilitating the preparation method of the Chinese medicine powder of melanin of black-bone chicken deposition as claimed in claim 4, which is characterized in that also
It is included in front of carrying out freeze thawing treatment to mixture, mixture is pre-processed, method particularly includes:
Dissolution liquid is added into mixture for 1:5 in mass ratio, after mixing evenly, positive electrode and negative electrode are inserted into, and connects
Current strength is the electric current of 10mA, then energization 50min is placed in that supersonic frequency is 80kHz, temperature is to be ultrasonically treated at 60 DEG C again
40min, wherein dissolution liquid specifically: in mass ratio by salt, sodium citrate, potassium dihydrogen phosphate, pineapple pulp juice, drinking water
It is uniformly mixed for 0.1:0.2:0.2:1:2;
Wherein, pineapple pulp is beaten filtering taking juice to get pineapple pulp juice.
8. Chinese medicine powder as claimed in any one of claims 2 to 7 is in the application for improving the Gallus domesticlus brisson cock melanin deposition that reaches the clouds.
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CN115005340A (en) * | 2022-04-01 | 2022-09-06 | 江西农业大学 | Application of phytol serving as feed additive for promoting melanin deposition in black-bone chicken feed |
CN115005340B (en) * | 2022-04-01 | 2023-11-07 | 江西农业大学 | Application of phyllanthin as feed additive for promoting melanin deposition in black-bone chicken feed |
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