CN103719497A

CN103719497A - Gynostemma pentaphylla fat-reducing and weight-losing health-care tea and preparation method thereof

Info

Publication number: CN103719497A
Application number: CN201310753087.5A
Authority: CN
Inventors: 不公告发明人
Original assignee: Qingdao Hengbo Instrument Co Ltd
Current assignee: Zhangjiajie Aishanyuan Tea Industry Co., Ltd.
Priority date: 2013-12-31
Filing date: 2013-12-31
Publication date: 2014-04-16
Anticipated expiration: 2033-12-31
Also published as: CN103719497B

Abstract

The invention provides gynostemma pentaphylla fat-reducing and weight-losing health-care tea which is characterized by comprising the following raw materials in parts by weight: 10-50 parts of gynostemma pentaphylla, 10-20 parts of angelica sinensis, 10-20 parts of salviae miltiorrhizae, 10-20 parts of semen plantaginis, 10-20 parts of caulis spatholobi, 10-20 parts of rhizoma alismatis, 10-20 parts of poria cocos, 10-20 parts of radix angelicae pubescentis, 10-20 parts of cortex acanthopanacis, 10-20 parts of astragalus membranaceus, 10-20 parts of astragalus membranaceus, 10-20 parts of radices trichosanthis, 10-20 parts of Scutellaria baicalensis, 10-20 parts of rhizoma atractylodis macrocephalae and 10-20 parts of lotus leaves, wherein gynostemma pentaphylla has the functions of remarkably reducing cholesterol (TCH) (Total Cholesterol), triglyceride (TG) and low density lipoprotein (LDL), raising high-density lipoprotein (HDL), protecting vascular endothelinal cells, preventing lipid from depositing on vascular walls and resisting arteriosclerosis as well as regulates blood pressure and prevents and treats thrombus. The gynostemma pentaphylla has a remarkable function of reducing blood viscosity and regulating blood pressure. Meanwhile, the gynostemma pentaphylla can prevent microthrombosis, and enhance the tolerance of myocardial cell to anoxia so as to protect the myocardium.

Description

A kind of gynostemma pentaphylla lowering blood-fat and reducing weight health protection tea and preparation method thereof

Technical field

The present invention relates to contain the health products that derive from plant composition, particularly a kind of gynostemma pentaphylla lowering blood-fat and reducing weight health protection tea and preparation method thereof.

Background technology

The high speed development of Modern Urban Living, also brings a series of rich man's diseases.Wherein " three height " and obesity are puzzlement people's primary enemies." three high diseases " refers to hypertension, hyperglycaemia (diabetes) and hyperlipemia.Conventionally be accompanied by obesity.They are " rich man's diseases " that modern society is derived from, and possible individualism also may be interrelated.As: diabetes patient is easy to suffer from hypertension or hyperlipemia simultaneously, and high fat of blood is the principal element of artery sclerosis formation and development, and the poor aggravation blood pressure of arteriosclerotic's blood vessel elasticity raises, so the later stage has all formed " three high diseases ".About three height, be what has statistics, in the Chinese ten large causes of death, the death rate relevant to metabolic disease is just up to 35.7%, and the death toll relevant to " three height " also accounts for 27% of total death toll.High fat of blood can cause blood vessel embolism; Hypertension can cause cerebral hemorrhage and rupture of blood vessel in brain; Hyperglycaemia can cause diabetes.The World Health Organization once clearly proposed, and prevented that the first line of defence of cardiovascular disease from reducing exactly " three height " and controlled " three height ".A lot of diabetes patients are with hyperlipidemia, so people call sister's disease diabetes and hyperlipidemia conventionally, and think that high fat of blood is the secondary disease of diabetes.About 40% diabetic has disorders of lipid metabolism according to statistics.Being characterized in that triglycerides increases with HDL reduces.

Among the people, gynostemma pentaphylla is used for the treatment of to high fat of blood, hypertension, hyperglycaemia, especially effective in cure significantly to high fat of blood, be that other medicine foods are difficult to substitute.China since the distributed resource of Gynostemma pentaphyllum Makino in 1984 investigate, the work such as exploitation.Through pharmacology, toxicity and clinical proof, gynostemma pentaphylla has " adaptation source " sample function, and multiple cancer cell is had to significant inhibitory action; In addition, gynostemma pentaphylla also has reducing blood lipid, hypotensive, hypoglycemic, calm, hypnosis, anti-anxiety, antiulcer, antifatigue, prolongation cell survival and improves a poor appetite, build up resistance, unobstructed stool, antiasthmatic-antitussive, the hemorrhoid that disappear, fat-reducing, the treatment chronic diseases marked by deficiency of vital energy, inhibition gall stone form, prevent and treat glucocorticoid side effect, the anti-ageing multiple efficacies of waiting for a long time.Except having the effect of ginseng, also there is the multiple effect that ginseng does not possess, and there is no reaction and any other toxic and side effect of ginseng excessive use.

Reducing blood lipid: gynostemma pentaphylla has remarkable reduction cholesterol (TCH), triglyceride (TG), low-density lipoprotein (LDL); increasing high density lipoprotein (HDL), protection blood vessel cell; stop lipid to deposit at vascular wall, the effect of anti arteriosclerosis.Adjust blood pressure control thrombus: gynostemma pentaphylla has obvious reduction blood viscosity, adjusts blood pressure function, can prevent that microthrombus from forming and increasing the tolerance of cardiac muscle cell to anoxic, plays the myocardium effect of protection simultaneously.Thrombus is caused by the multiple factor, internal and external test all proves, the thrombus that Herb Gynostemmae Pentaphylli extract forms the multiple factor has good depolymerization or inhibit feature, shows that gynostemma pentaphylla is the anti-thrombosis drug with a plurality of link functions such as suppressing platelet function, coagulation function and erythrocyte aggregation.Control cardiovascular disease: Herb Gynostemmae Pentaphylli extract has significant protective effect to Myocardial Infarction: can dwindle myocardial infarct size; after suppressing miocardial infarction, free fatty (FFA) raises; reduce MDA (MDA) content; protection SOD of heart tissue and creatine phosphokinase (CPK) activity, the metabolic disorder of FFA while correcting myocardial ischemia.Therefore, myocardial ischemia and Ischemia Reperfusion damage are had to good protective effect.The regulating lipid metabolism of gynostemma pentaphylla and antioxidation are all of value to atherosclerotic control, and clinical testing has also proved the good result of Jiaogulan in treating coronary heart disease.Regulate blood sugar: gynostemma pentaphylla can be protected adrenal gland and thymus gland and endocrine organ, maintains the normal function of internal system.Just because of this effect of gynostemma pentaphylla, so it has hypoglycemic and improves carbohydrate metabolism.Regulate human physiological functions: gynostemma pentaphylla can be protected the unlikely atrophy with the growth at age of adrenal gland and thymus gland and endocrine organ, maintains the function of internal system, and have hypoglycemic and improve carbohydrate metabolism.It is raw material that " gynostemma pentaphylla " blade is take in the present invention, and wherein bud-leaf effect is best, and pure manual harvesting, is combined and processes with prepared slices of Chinese crude drugs technique through ancient method tea-manufacturing technology.Soup look dark green, and delicate fragrance overflows, micro-bitter glycol.

It is all in order to improve the health that modern selects some health foods or functional food.The function of health products is mainly to improve brain blood circulation, adheres to physical training and suitably applies psychotropic agent.Dragons and fishes jumbled together for various health products, the medicine sold on the market now, great majority are the amusement of false propaganda just, what have does not only have a health-care components, also cause the huge injury of the person, therefore, the effective softening blood vessel method of search of health has very real significance, and softening blood vessel active material is that people expect the key of dealing with problems always safely and effectively.Traditional Chinese medicine capable of reducing weight lipopenicillinase, have advantages of nontoxic, can be absorbed completely by organism, be the main direction that we study.Now market is in the urgent need to developing a kind of have reducing blood lipid, antibacterial, hypoglycemic, antioxidation, and can interference body to fatty absorption, the product that the fat that promotes to take in excretes, and have advantages of nontoxic, can be absorbed completely by organism.

Summary of the invention

Technical problem to be solved by this invention is, dragons and fishes jumbled together for most of diet products in market now, and the overwhelming majority is simple cathartic, allow people dewater in a large number, can cause huge injury to the person, therefore, the effective slimming health product of search of health has very real significance.Gynostemma pentaphylla has reducing blood lipid, hypotensive, hypoglycemic, calm, hypnosis, anti-anxiety, antiulcer, antifatigue, prolongation cell survival and improves a poor appetite, build up resistance, unobstructed stool, antiasthmatic-antitussive, the hemorrhoid that disappear, fat-reducing, the treatment chronic diseases marked by deficiency of vital energy, inhibition gall stone form, prevent and treat glucocorticoid side effect, the anti-ageing multiple efficacies of waiting for a long time; The present invention promotes body fat metabolism and makes it balance, thereby reaches reducing blood lipid, reverses the foster effects such as fatty liver, and simultaneously to fat-reducing, defaecation, toxin expelling, short sleeper effect is definite.Add that some are promoting blood circulation and removing blood stasis, fat and serum triglyceride are all had to the Chinese medicine of obvious reducing effect, and can improve microcirculation, make people be easy to acceptance, reach the function of daily health caring, more can reach preferably the effect of Weight-reducing and lipid-lowering.

For solving above-mentioned technical barrier, the invention provides a kind of gynostemma pentaphylla lowering blood-fat and reducing weight health protection tea, described in it, in gynostemma pentaphylla lowering blood-fat and reducing weight health protection tea, the parts by weight of various raw materials are: 10～50 parts of gynostemma pentaphylla, 10～20 parts of Radix Angelicae Sinensis, 10～20 parts of the reds sage root, 10～20 parts of plantain seeds, 10～20 parts of reticulate millettias, 10～20 parts of rhizoma alismatis, 10～20 parts, Poria cocos, 10～20 parts of levisticums, 10～20 parts of cortex acanthopanacis, 10～20 parts of the Radixs Astragali, 10～20 parts of Ligusticum wallichiis, 10～20 parts of roots of Chinese trichosanthes, 10～20 parts of the roots of large-flowered skullcap, 10～20 parts of the bighead atractylodes rhizomes, 10～20 parts, lotus leaf.

Described gynostemma pentaphylla lowering blood-fat and reducing weight health protection tea, described in it, in gynostemma pentaphylla lowering blood-fat and reducing weight health protection tea, the parts by weight of various raw materials can be: 10～30 parts of gynostemma pentaphylla, 10～15 parts of Radix Angelicae Sinensis, 10～15 parts of the reds sage root, 10～15 parts of plantain seeds, 10～15 parts of reticulate millettias, 10～15 parts of rhizoma alismatis, 10～15 parts, Poria cocos, 10～15 parts of levisticums, 10～20 parts of cortex acanthopanacis, 10～20 parts of the Radixs Astragali, 10～20 parts of Ligusticum wallichiis, 10～20 parts of roots of Chinese trichosanthes, 10～20 parts of the roots of large-flowered skullcap, 10～20 parts of the bighead atractylodes rhizomes, 10～20 parts, lotus leaf.

Described gynostemma pentaphylla lowering blood-fat and reducing weight health protection tea, described in it, in gynostemma pentaphylla lowering blood-fat and reducing weight health protection tea, the parts by weight of various raw materials can also be: 10～40 parts of gynostemma pentaphylla, 10～20 parts of Radix Angelicae Sinensis, 10～20 parts of the reds sage root, 10～20 parts of plantain seeds, 10～20 parts of reticulate millettias, 10～20 parts of rhizoma alismatis, 10～20 parts, Poria cocos, 10～20 parts of levisticums, 10～15 parts of cortex acanthopanacis, 10～15 parts of the Radixs Astragali, 10～15 parts of Ligusticum wallichiis, 10～15 parts of roots of Chinese trichosanthes, 10～15 parts of the roots of large-flowered skullcap, 10～15 parts of the bighead atractylodes rhizomes, 10～15 parts, lotus leaf.

For solving the problems of the technologies described above, the present invention also provides the preparation method of a kind of gynostemma pentaphylla lowering blood-fat and reducing weight health protection tea, and described in it, the preparation process of gynostemma pentaphylla lowering blood-fat and reducing weight health protection tea comprises following aspect:

A. the preparation of gynostemma pentaphylla;

B. the gynostemma pentaphylla Titian preparing;

C. described other raw material soaking ethanol heating are extracted;

D. the liquid again surplus stock being extracted is condensed into granule, pulvis or paste;

E. concentrated granule, pulvis or paste are mixed with the gynostemma pentaphylla preparing, sterilizing is prepared into health care medicinal tea;

F. 1～2 gram of the present invention is brewed to 250 milliliters of boiling water, can repeatedly brew more than 10 times.

In described step a, the fresh and tender gynostemma pentaphylla of manual harvesting, airing, parches; The good gynostemma pentaphylla of airing is put into the pot that completes and go green grass or young crops, the 30-45 minute that completes at 70-100 ℃, then puts into large iron pan and stir-fries, first downwards backward upper, firmly wants evenly, when gynostemma pentaphylla color starts jaundice, starts the moulding in 20-30 minute that stir-fries of little fire.

In described step b, the gynostemma pentaphylla of having fried can be put into fragrance extracting machine Titian 30-45 minute, after taking out, naturally dry.

In described step c, can get in the ethanol that other raw materials add 5-10 doubly to measure and soak 1-2 hour, heating is extracted 2 times, and each 1-2 hour, removes supernatant, merge extract, 100-120 order filters, then is the ultrafiltration post ultrafiltration of 5000-10000 through the molecular weight that dams, 1.36 medicinal extract when ultrafiltrate reduced pressure concentration relative density is 80 ℃, heating is concentrated into paste, stand for standby use.

In described steps d, concentrated medicinal extract can be inserted in economic benefits and social benefits vacuum concentrator to the concentrate that while being concentrated into 90 ℃, relative density is 1.05, set to 0～5 ℃ of deepfreezes 24 hours; Refrigeration liquid is added to 0.3% filter aid diatomite, filter, filtrate is inserted in economic benefits and social benefits vacuum concentrator again, is concentrated into every 1ml containing 1g crude drug amount; Paste after concentrated adds the agent of honey mediation granulation; Or convection drying is made pulvis.

In described step e, the gynostemma pentaphylla making can be mixed with other raw materials of refinement, after ultraviolet ray sterilization bactericidal, pack.

For solving the problems of the technologies described above, the present invention also provides the preparation method of a kind of gynostemma pentaphylla lowering blood-fat and reducing weight health protection tea, and its preparation process can also be: get bulk drug and clean and to dry, in 70 ℃ of baking ovens, dry 8-12h, taking-up is placed in drying box cooling, with pulverizer, pulverizes 80-100 mesh sieve; The particle that sieves is soaked in water 12 hours, then put into extractor heating and boil 1 hour, filter filtrate for later use; Filter residue adds water, and heating, boils 45 minutes for the second time, filters filtrate for later use; Filter residue adds water again, and heating is for the third time boiled half an hour, filters; Three filtrates are combined, and powder is placed in 70-90% ethanol and soaks 1-2 hour, is heated to 60 ℃-70 ℃ in water-bath, lixiviate 2h, every 10min stirs once, by filtered through gauze, in residue, add 50-60% ethanol, 60 ℃-70 ℃ are continued lixiviate 2h, and every 10min stirs once, filtered through gauze, merge leaching liquor, be condensed into pasty state, by pasty state extract mediation honey or dextrin, granulation agent; Or convection drying is made pulvis.

The technique effect that the present invention is useful is, it is raw material that " gynostemma pentaphylla " blade is take in the present invention, and wherein bud-leaf effect is best, and pure manual harvesting, is combined and processes with prepared slices of Chinese crude drugs technique through ancient method tea-manufacturing technology.Soup look dark green, and delicate fragrance overflows, micro-bitter glycol.Gynostemma pentaphylla has reducing blood lipid, hypotensive, hypoglycemic, calm, hypnosis, anti-anxiety, antiulcer, antifatigue, prolongation cell survival and improves a poor appetite, build up resistance, unobstructed stool, antiasthmatic-antitussive, the hemorrhoid that disappear, fat-reducing, the treatment chronic diseases marked by deficiency of vital energy, inhibition gall stone form, prevent and treat glucocorticoid side effect, the anti-ageing multiple efficacies of waiting for a long time; The present invention promotes body fat metabolism and makes it balance, thereby reaches reducing blood lipid, reverses the foster effects such as fatty liver, and simultaneously to fat-reducing, defaecation, toxin expelling, short sleeper effect is definite.Add that some are promoting blood circulation and removing blood stasis, fat and serum triglyceride are all had to the Chinese medicine of obvious reducing effect, and can improve microcirculation, make people be easy to acceptance, reach the function of daily health caring, more can reach preferably the effect of Weight-reducing and lipid-lowering.Research shows: gynostemma pentaphylla, except containing gynosaponin, also contains Flavonoid substances, polysaccharide, phosphatide, amino acid, vitamin, constant and micro-inorganic elements isoreactivity composition.

The specific embodiment

Along with industrialized development, the variation of dietary structure, the improvement of living condition, Overweight and obesity crowd's quantity worldwide increases rapidly, and be the trend of becoming younger, become the global healthy epidemic disease that has a strong impact on, therefore, the effective method of weight-reducing of search of health has very real significance, and the active material of losing weight is safely and effectively the key that people expect to solve problem of obesity always.Hypoglycemic method is a lot, has dietetic treatment, kinesiatrics, medicinal treatment and clinical treatment etc. and these methods more or less have weak point, and what have also easily causes side effect, as diarrhoea, apocleisis etc.And that health treatment fat-reducing has is easy to use, have no side effect, the feature such as effect is remarkable, and extremely consumer's favor.

Can subtract 10 jin of above methods of weight-reducing January innumerable.The most effective method of weight-reducing, be not this month you deduct 10 jin, 20 jin, 30 jin of body weight, but lose weight specifically, you are thin has got off, you are bounce-back not, you have subtracted more healthy and more healthy, otherwise cut, how much all deceive oneself as well as others.Fat-reducing is a process that relatively has the time limit just, and control body weight through people's all one's life, add and cause that fat reason is many-sided, as: overweight people's age, sex, with chronic disease, health, life and eating habit, many-sided many difference existing such as inherent cause, so fat-reducing is not a simple thing, not only to there is the method for science, certain willpower, more need professional person's guidance and help, if pay no attention to anti-obesity mechanism, pay no attention to s own situation, blindness is simply followed the wind, be tantamount to body reagent, trying by chance right that is fortune, unfortunately fortune can not bring everyone, in the time of more, what the method for weight-reducing of mistake was brought can only be wrong result.How should we subtract so? find out self fat root, suit the remedy to the case.Have lipodystrophy, good, we offer its sufficient nutrition, activate it, otherwise the heat or eat slimming drugs of cutting down one's diet simply, only can make this obstacle make the matter worse, your oil supply not of machine, light is want to rotate it by external force, and result well imagines, people's cell too.Of excessive eating and drinking, take in heat too high, good, we must control the diet of controlling oneself, and low-keyed eating, selectively eats, and free eats, rather than suffering from hunger simply.

Due to the prosperity day by day of economy, the continuous lifting of modern's living standard, causes too much the having deep love for of people to be enjoyed, and does not note oneself life style, behavioural habits, diet hobby, thus the disease causing.Society with fast rhythm's life, the operating pressure of keen competition, modern's physical and mental health has been caused to serious threat, make people more than half in sub-health state, show as: dizziness, nervous, weak, poor appetite, failure of memory, insomnia, indifferent to etc. to thing, in addition, long-term diet is unreasonable, that is: smart grain replaces coarse food grain completely, three a large amount of height (high heat, high protein, higher fatty acid), more than three, (anticorrisive agent is many, flavoring agent is many, hormone is many) residual agricultural chemicals in the absorption of food and food, chemical fertilizer stockpiling in vivo, add air, pollution of waterhead and amount of exercise are less than normal, go down to make three high disease (hypertension for a long time like this, hyperglycaemia, high fat of blood), cardiovascular and cerebrovascular disease, cancers etc. so-called " modern civilization diseases " rise rapidly, and constantly rejuvenation, the serious health and lives that jeopardizes the mankind.

Through pharmacology, toxicity and clinical proof, gynostemma pentaphylla has " adaptation source " sample function, and multiple cancer cell is had to significant inhibitory action; In addition, gynostemma pentaphylla also has reducing blood lipid, hypotensive, hypoglycemic, calm, hypnosis, anti-anxiety, antiulcer, antifatigue, prolongation cell survival and improves a poor appetite, build up resistance, unobstructed stool, antiasthmatic-antitussive, the hemorrhoid that disappear, fat-reducing, the treatment chronic diseases marked by deficiency of vital energy, inhibition gall stone form, prevent and treat glucocorticoid side effect, the anti-ageing multiple efficacies of waiting for a long time.Except having the effect of ginseng, also there is the multiple effect that ginseng does not possess, and there is no reaction and any other toxic and side effect of ginseng excessive use.Reducing blood lipid: gynostemma pentaphylla has remarkable reduction cholesterol (TCH), triglyceride (TG), low-density lipoprotein (LDL); increasing high density lipoprotein (HDL), protection blood vessel cell; stop lipid to deposit at vascular wall, the effect of anti arteriosclerosis.Adjust blood pressure control thrombus: gynostemma pentaphylla has obvious reduction blood viscosity, adjusts blood pressure function, can prevent that microthrombus from forming and increasing the tolerance of cardiac muscle cell to anoxic, plays the myocardium effect of protection simultaneously.Thrombus is caused by the multiple factor, internal and external test all proves, the thrombus that Herb Gynostemmae Pentaphylli extract forms the multiple factor has good depolymerization or inhibit feature, shows that gynostemma pentaphylla is the anti-thrombosis drug with a plurality of link functions such as suppressing platelet function, coagulation function and erythrocyte aggregation.Control cardiovascular disease: Herb Gynostemmae Pentaphylli extract has significant protective effect to Myocardial Infarction: can dwindle myocardial infarct size; after suppressing miocardial infarction, free fatty (FFA) raises; reduce MDA (MDA) content; protection SOD of heart tissue and creatine phosphokinase (CPK) activity, the metabolic disorder of FFA while correcting myocardial ischemia.Therefore, myocardial ischemia and Ischemia Reperfusion damage are had to good protective effect.The regulating lipid metabolism of gynostemma pentaphylla and antioxidation are all of value to atherosclerotic control, and clinical testing has also proved the good result of Jiaogulan in treating coronary heart disease.Regulate blood sugar: gynostemma pentaphylla can be protected adrenal gland and thymus gland and endocrine organ, maintains the normal function of internal system.Just because of this effect of gynostemma pentaphylla, so it has hypoglycemic and improves carbohydrate metabolism.Regulate human physiological functions: gynostemma pentaphylla can be protected the unlikely atrophy with the growth at age of adrenal gland and thymus gland and endocrine organ, maintains the function of internal system, and have hypoglycemic and improve carbohydrate metabolism.It is raw material that " gynostemma pentaphylla " blade is take in the present invention, and wherein bud-leaf effect is best, and pure manual harvesting, is combined and processes with prepared slices of Chinese crude drugs technique through ancient method tea-manufacturing technology.Soup look dark green, and delicate fragrance overflows, micro-bitter glycol.

Plantain seed returns lung channel; Liver Channel; Kidney channel; Urinary bladder channel.Function: reducing fever and causing diuresis; Excreting dampness antidiarrheal; Improving eyesight; Eliminate the phlegm.Cure mainly: main difficult urination; Under stranguria with turbid discharge band; Oedema turgor; Summer-heat and damp is rushed down dysentery; Hot eyes is hidden from view; Phlegm heat is coughed and is breathed heavily.1. diuresis, normal person is taken plantain seed decoction 10g orally, has diuresis, but enters decoction total capacity and every day the water yield all without recording, and lacks strictly contrast.Normal person and rabbit take Asiatic plantain seed decoction without obvious diuresis.Before flatcar, seed is also without diuresis.The < < property of medicine > > that falls: can remove wind poison, hepatic stroke heat, malicious wind dotting punch order, red pain is hidden from view, and ambition dysphoria with smothery sensation is gone in cerebralgia lacrimation.< < Japan hanako materia medica > >: diuresis drenches puckery, establishing-Yang.Control de-essence, vexed.Lower gas.

Radix Angelicae Sinensis, its taste is sweet and weigh, therefore specially can enrich blood, its gas is light and pungent, thus again can promoting circulation of blood, bowl spares has, in row, has benefit, really the gas medicine in blood, the also panacea in blood.About mending to mend of assistant, thus can support nutrition blood, tonifying Qi production of sperm, five viscera settling, body by force, beneficial mind, all tangible deficient diseases, nothing is unsuitable.Assistant logical to attack, therefore the pain defaecation of dispelling, relieving rigidity of muscles and joints, controls contraction, paralysis, the card such as dry, puckery.Seek void and exterior syndrome being not relieved person, help with agent such as bavin, Pueraria lobota, fiber crops, osmanthus, the Biao Weire that can fall apart greatly, and table is not held back person, and assistant, with rheum officinale and so on, can be shown again admittedly.Precisely because gas Xin Erdong, thus want its quiet person when keeping away it, the sliding benefaction of property, stool is not consolidated person when keeping away it.All cloudy moderate heat Sheng persons, the active blood of Radix Angelicae Sinensis, also non-institute is suitable, cloudy deficiency of YANG of middle-JIAO person, Radix Angelicae Sinensis can nourish blood, and is indispensable.

The red sage root is bitter, is slightly cold.The thoughts of returning home, Liver Channel.Stasis-dispelling and pain-killing, activating blood to promote menstruation, relieving restlessness clears away heart-fire.For irregular menstruation, through closing dysmenorrhoea, a lump in the abdomen causing distension and pain, chest ventral spine pain, and hot numbness pain, sore swells and ache, dysphoria and insomnia.For sternal rib hypochondriac pain, arthralgia due to wind-dampness, abdominal mass caking, sore swells and ache, fall fall forward grieved, irregular menstruation, through closing dysmenorrhoea, postpartum stasis of blood pain etc.Treatment sternal rib pain, abdominal mass caking, and irregular menstruation, through amenorrhoea, bitterly there is good effect.< < detailed outline > > carries: the red sage root, press the sensible opinion of < < married woman > > cloud, Siwu Tang is controlled gynecopathy, pay no attention to antenatal postpartum, how many through water, all can be general, the only taste red sage root is loose, cures mainly identical with it.The lid red sage root can break place blood, mends new blood, peaceful tire, the stillborn foetus that falls, stop metrorrhagia stagnant under, menstruation regulating arteries and veins.Main Compositions of Radix Salviae Miltiorrhizae tanshinone has hemangiectasis, improves circulation, removes the effects such as intravascular coagulation, has the effect of invigorating blood circulation trace, and can promote liver blood circulation, is beneficial to liver cell regeneration.

Reticulate millettia property is bitter, sweet, temperature.Return liver, kidney channel, enrich blood, invigorate blood circulation, dredging collateral.For irregular menstruation, the deficiency of blood is sallow, paralysis and numbness, and arthralgia due to wind-dampness is activated blood and relaxed tendons; Nourishing blood for regulating menstruation, < < A Supplement to the Compendium of Materia Medica > > carries it and can cure numb in every limb; Quadriplegia; Arthralgia due to wind-dampness; Women has through uncomfortable; Dysmenorrhoea.External application is the gynaecology's good medicine activating blood circulation and dispersing blood clots.

Poria cocos taste is sweet, light, property is flat, be used as medicine there is clearing damp and promoting diuresis, the function of beneficial spleen and stomach, antitoxic heart-soothing and sedative.Modern medicine study: Poria cocos energy enhanced machine body immunity function, pachymaran has significantly antitumor and protect the liver dirty effect.Poria cocos slightly sweet flavor is flat, enters the heart, lung, the spleen channel.There is eliminating dampness and diuresis, strengthening the spleen and stomach, the effect of antitoxic heart-soothing and sedative.Can control difficult urination, oedema turgor, phlegm and retained fluid is coughed contrary, vomits contrary, and vomiting during early pregnancy, has loose bowels, seminal emission, stranguria with turbid discharge, palpitation with fear, forgetful disease such as grade.The Li Shui of Poria cocos, reaches by strong fortune spleen PFT, different from the Chinese medicine of other direct Li Shui.For splenasthenic diarrhea, the lower Poria cocos of band can invigorating the spleen, again can excreting dampness, and due to insufficiency of the spleen dysfunction of the spleen in transportation and transformation, to have loose bowels, be with down, application Poria cocos has the effect of giving consideration to both the incidental and fundamental, the compatibilities such as normal and Radix Codonopsis, the bighead atractylodes rhizome, Chinese yam.Can be used as tonifying lung spleen, control the adjuvant of the deficiency of vital energy.

Ligusticum wallichii is usually used in blood-activating and qi-promoting, wind-expelling pain-stopping, Main Cultivation in Sichuan, the ground such as Yunnan, Guizhou, Guangxi, Hubei.The pungent temperature of Ligusticum wallichii is fragrant dry, walks and does not keep, can go loose, the up top, mountain peak that reaches; Enter again blood system, the descending sea of blood that reaches.Promoting blood circulation effect is extensive, the various illnesss of suitable stagnation of blood stasis; Wind-expelling pain-stopping, effectiveness is excellent, can control the diseases such as a wind headache, arthralgia due to wind-dampness.It is the gas medicine in blood that former times people calls Ligusticum wallichii, nearly says the functions such as pungent loose, the Xie Yu of its residence, sensible, pain relieving.2.1 pairs of action of the heart Ligusticum wallichii decoctions of effect of cardiovascular system, to Isolated Toad and frog heart, are made in concentration to contractive amplitude increases, heart rate is slightly slow during at 10-5-10-4.Press the test of EnglmannShi method, Ligusticum wallichii 20 or 30g/kgig, also make frog heart amplitude increase in place, decreased heart rate; With 40g/kgig, can make the frog heart stop fighting.Ligustrazine iv also has cardiotonic to anesthetized dog, with heart rate, accelerates.Remove vagal heart, ligustrazine is still had to significant reaction.Vertebral artery injects larger dose ligustrazine, and its Cardiovascular is not obvious.Award in advance inderal or reserpinization, can eliminate ligustrazine completely to action of the heart, so ligustrazine is to action of the heart, may be by due to sympathetic nerve indirect excitation heart B acceptor.< < detailed outline > >: eliminating dampness, antidiarrheal dysentery, activating QI to alleviate the depression.Rhizome of chuanxiong Qiong, in blood gas medicine also, liver bitter anxious with pungent benefit it, therefore deficiency of blood person should it; Pungent to fall apart it, therefore obstruction of the circulation of vital energy person should it.

Rhizoma alismatis (rhizome) is one of traditional Chinese medicine.Theory of traditional Chinese medical science thinks that it is cold in nature, has the effect of clearing damp and promoting diuresis.Modern medicine study, rhizoma alismatis can reduce serum total cholesterol and triacylglycerol content, slows down atherogenesis; Rhizoma alismatis and preparation modern times thereof are also used for the treatment of auditory vertigo, dyslipidemia, seminal emission, fatty liver and diabetes etc.The fat-soluble part of rhizoma alismatis has obvious norcholesterol effect and study of anti-atherogenic effect to experimental hypercholesterolemicrabbits rabbit, by wherein separated alisol A, B and alisol A, the acetic acid esters of B, C, except alisol B, there is the norcholesterol effect showing.Content with 0.1% adds in the feed of experimental atherosclerosis rats, can make cholesterolemia decline more than 50%, wherein the strongest with the effect of alisol A-24-acetic acid esters.The ethanol extract of rhizoma alismatis, the ethyl acetate extract of ethanol extract etc., have effect for reducing blood fat to experimental hypercholesterolemicrabbits rabbit and rat.Ethyl acetate extract and its residual fraction effect being insoluble in acetic acid one water are the strongest.Ethyl acetate extract oral 1g/kg every day, also has obvious norcholesterol effect to the normal rat of raising with normal diet.By coordination list notation method, prove, alisol A has the ability that suppresses mouse small intestine esterified cholesterol, and can make the absorptivity of cholesterol in rat small intestine reduce by 34%, but not affecting linoleic absorption gynostemma pentaphylla finds through modern medicine study, the main active ingredient of gynostemma pentaphylla is polysaccharide, flavonoids, saponins and microelement kind, its effect is mainly to promote body fat class metabolism, nutrition human body cell, there is good detoxicating, relieving inflammation effect simultaneously, be widely used in high fat of blood, fatty liver, fat, constipation, insomnia, hepatitis B, chronic respiratory inflammation (sphagitis, enterogastritis etc.) treatment and health care.

Levisticum, pungent, bitter, tepor.Return liver, kidney, urinary bladder channel.Cure mainly expelling wind and eliminating dampness; Eliminating cold to stop pain.For wind-cold-dampness arthralgia; Lumbocrural pain; Few cloudy volt wind headache, headache dentalgia, has the immunity of enhancing, anti-inflammatory, the pharmacological action such as antibacterial, is the medicine simply of commonly using in tcm clinical practice.< < book on Chinese herbal medicine converges says > >: levisticum, and benefaction blood system, the wet loose cold medicine of the row that dispels the wind is also.The card of all sick wind, can not pitching as head term, and waist knee can not bend and stretch, or numbness is difficult to walk bitterly, numbness need not, all wind with cold due to, heat is hindered also with wet institute; Must be by the toil of levisticum and temperature, movable qi and blood, loose cold-evil dispels, the beriberi therefore < < book on Chinese herbal medicine > > speech can fall apart, changes the globefish that runs quickly, and treats hernia lump in the abdomen, subduing inflammation, control harmful wind hundred joints and attack pain, surely lack the strongly fragrant headache of cold sensation of the genitalia, be intended to this.< < medicineization justice > >: levisticum, can declare airway, certainly push up to knee, with the kidney channel volt wind that falls apart, all necks are difficult to relax, buttocks and legs pain, two sufficient impotence numbness, actively do not move, non-this efficiency is not.Can control wind, wind wins wet, specially dredges moisture, if back acid is heavy, and four limbs contraction impotence, flesh Huang is made piece, is called good dose.Helping again blood medicine, activate blood and relax tendons, is very wonderful.

The root skin that cortex acanthopanacis Araliaceae machaka acanthopanax gracilistylus and Panax sessiliflorus are dry.Have another name called white thorn, order bone, chase after that wind makes, CORTEX ACANTHOPANACIS etc.Main product in Hubei, the ground such as Hunan, Henan, Anhui, Sichuan.Chinese medicine cortex acanthopanacis has wines used as antirheumatic, filling liver kidney, the effects such as strengthening the bones and muscles.In < < Compendium of Materia Medica > >, record: " tonifying middle-Jiao and Qi, control rheumatism impotence numbness, strengthen the bone." modern study, the effect such as that this product also has is antitumor, antifatigue, reduction WBV, atherosclerosis formation.Cortex acanthopanacis is easy to use, decoct soup, enter ball, San Huojin slender acanthopanax bark spirit all can, but fire excess from yin deficiency person is cautious use of.

The Radix Astragali has the effects such as invigorating qi for strengthening superficies, sharp water detumescence, pus draining and toxin expelling, myogenic.The medicinal history of existing more than 2000 year so far of the Radix Astragali, modern study, the Radix Astragali is containing various trace elements such as saponin, sucrose, polysaccharide, several amino acids, folic acid and selenium, zinc, copper.Have enhanced machine body immunity function, protect the liver, diuresis, anti-ageing, anti-stress, step-down and antibacterial action more widely.But table is real evil, contain, retention of dampness due to stagnation of QI, dyspepsia is stagnated, ulcer from the beginning of or the real example such as routed after heat poison Shang Sheng.The Radix Astragali has invigorating qi for strengthening superficies, diuresis holder poison, apocenosis, the effect of expelling pus and promoting granulation.Weak for the deficiency of vital energy, anorexia and loose stool, the sinking of qi of middle-jiao, rushes down prolapse of the anus for a long time, the uterine bleeding of having blood in stool, exterior deficiency spontaneous perspiration, ulcer is difficult bursts, and bursts and does not hold back for a long time, and the deficiency of blood is sallow, and interior heat is quenched one's thirst.< < herbal classic > > records: " main ulcer, loses sore for a long time, apocenosis pain relieving.Qi-restoratives, children's's all kinds of diseases and ailments." < < Japan hanako materia medica > > record: " help gas to strengthen the bone, put on flesh and enrich blood.”

Root of Chinese trichosanthes is the root of cucurbitaceous plant Chinese juniper beach wormwood, is a kind of Chinese medicine, is clearing heat-fire class medicine, and its concrete effect is clearing heat-fire, promote the production of body fluid to quench thirst, and apocenosis detumescence.Cure mainly: control pyreticosis thirsty, quench one's thirst, jaundice, dryness of the lung hemoptysis, carbuncle are swollen, hemorrhoid impotence.For treatment diabetes, conventional it and drugs for nourishing yin are used in conjunction with, to reach the effect for the treatment of both principal and secondary aspect of disease.< < book on Chinese herbal medicine converges says > >: root of Chinese trichosanthes, move back the five internal organs hot and suffocating, as internal heat, contain and dry tongue and mouth, lung-fire is contained and the swollen larynx numbness of pharynx, spleen fire is contained and dispute tooth is swollen, and convulsive seizure due to phlegm-fire is contained and cough is not peaceful.If the side of body of irascibility is swollen, walk note, the hectic fever due to yin dysphoria with smothery sensation of kidney fire, or ulcer bursts and do not burst, and hot poison is loose, or five subcutaneous ulcer yellow skin and eyes, and if if little water drenches puckeryly, be due to all burning hot pent-up, only convulsive seizure due to phlegm-fire, falls in this agent energy relieving stagnation, and can control it.Its property is sweet cold again, kindly can control yearningly, controls empty yearningly from tonic, controls fire thirsty from the medicine of a cold nature, controls strongly fragrant yearningly from gas medicine, from blood medicine, controls polydipsia, is to control thirsty key medicine also.

The root of large-flowered skullcap: the root of large-flowered skullcap has heat-clearing and damp-drying drug, cool blood is antiabortive, detoxicating functions.Cure mainly the diseases such as warm heat disease, the infection of the upper respiratory tract, cough with lung heat, damp and hot jaundice, pneumonia, dysentery, hemoptysis, hot eyes, fetal irritability, hypertension, the swollen furuncle sore of carbuncle.The clinical practice of the root of large-flowered skullcap is antibacterial not worse than the coptis, and does not develop immunity to drugs.We are used in fungal culture miscellaneous bacteria and infect special severity by the strong feature of broad-spectrum antibacterial action, fine by root of large-flowered skullcap extract effect.< < Bencao Jingshu > >: the root of large-flowered skullcap, its property is respectful clearly, so evil removing: so bitter eliminating dampness; So cold sensation of the genitalia victory heat, therefore main all heat.All hot persons, heat symptoms caused by an exopathgen with all over heat also, jaundice, intestines be out-of-the-way, let out dysentery, the disease of all damp and hot victory also, is analysed it, all diseases are from recover.Bitter cold can be except damp and hot, thus small intestine profit and water from by, source is clearly flowed also clean.The blood person of closing, real heat is at blood system, and instant heating human blood chamber, makes us obstructed through closing, damp and hot solution, Ying Qi is clearly and voluntarily also.Dislike sore subcutaneous ulcer erosion person, blood-head is stayed knot, and is that the swollen stain of carbuncle is also rotten: fiery ulcer person, internal heat blood trouble also, cool blood heat extraction, self-healing also.The root of large-flowered skullcap has antiallergic action and antiinflammatory action: the contained baicalin of the root of large-flowered skullcap and baicalein can obviously suppress the anaphylaxis contractile response that sensitized guinea pig rat small intestine in vitro and isolated tracheal produce antigen, to the passive general allergy of cavy and mouse and cavy passivity skin allergic reaction, baicalin and baicalein also have inhibitory action.The root of large-flowered skullcap has refrigeration function, most evidences, and the effective ingredient baicalein of the root of large-flowered skullcap has refrigeration function.The root of large-flowered skullcap has detoxication, and baicalin has obvious detoxication, and this may be that baicalin decomposites glucuronic acid due under the effect of glucuronidase, and due to being combined with multiple poisonous substance.10 milligrams of LD50 that can improve strychnine of baicalin reach 2.5 times, and the hepatic glycogen content of carbon tetrachloride poisoning mouse is increased.

Bighead atractylodes rhizome bitter, sweet, temperature.Return spleen, stomach warp.Cure mainly strengthening the spleen and replenishing qi, eliminating dampness Li Shui, hidroschesis, antiabortive.Few for insufficiency of the spleen food, abdominal distension is had loose bowels, and phlegm and retained fluid is dizzy throbs with fear, oedema, spontaneous perspiration, fetal irritability.The bighead atractylodes rhizome has CDCC to oncocyte, can reduce the rate of increase of oncocyte, lowers the aggressive of tumor tissue, improves the ability of the antitumor reaction of body.Baizhu volatile oil has inhibitory action to mouse EC, lymphosarcoma ascitic type, the cancer of the esophagus (Ecal09), sarcoma 180 etc.Atractylodes lactone B lumbar injection is to the murine sarcoma people.Also there is remarkable inhibitory action.< < property of medicine opinion > > carries it: monarch, taste is sweet, pungent, nontoxic.Can main large wandering arthritis, diarrhea due to disorder of qi for many years, trusted subordinate's distending pain, the broken place food that disappears, appetizing, removes sputum, except fever and chills, lets out under only.Interarea light is happy, preserves youthful looks, and de-black.The swelling of harnessing the river is full, tells to vomit contraryly, and crymodynia in abdomen, vomits and diarrhoea incessantly, and the cold dysentery of stomach Qi deficiency.

Lotus leaf contains the multiple alkaloids such as nuciferine (Roemerine), miltanthin (Pronuciferine) and Nuciferine (Nuciferine) and vitamin C, polysaccharide.There is the effect of clearing heat and detoxicating, cool blood, hemostasis.Modern study shows, lotus leaf also has effect for reducing blood fat, so all contain lotus leaf in a lot of fat-reducing, lipopenicillinase, product for resolving poxes.Effect for reducing blood fat, Chinese medicine modern study result shows, lotus leaf has effect for reducing blood fat.Lotus leaf decoction treatment hyperlipidemia, 1 20 days course for the treatment of, norcholesterol total effective rate reaches 91.3%, and wherein effective 37.8%.Have document announcement, the alkaloid in lotus leaf has effect for reducing blood fat, and is usually used in clinically the treatment of obesity.

Specific embodiment 1:

First by gynostemma pentaphylla frying, Titian.

Again by all the other raw material Radix Angelicae Sinensis, the red sage root, plantain seed, reticulate millettia, rhizoma alismatis, Poria cocos, levisticum, cortex acanthopanacis, the Radix Astragali, Ligusticum wallichii, root of Chinese trichosanthes, the root of large-flowered skullcap, the bighead atractylodes rhizome, lotus leaf extracts, and makes paste, granule or pulvis.

Two kinds of extracts mix, ultraviolet radiation for sterilizing and disinfecting pack.

Specific embodiment 2:

The fresh and tender gynostemma pentaphylla 5000g of manual harvesting, airing, parches; The good gynostemma pentaphylla of airing is put into the pot that completes and go green grass or young crops, the 30-45 minute that completes at 70-100 ℃, then puts into large iron pan and stir-fries, first downwards backward upper, firmly wants evenly, when gynostemma pentaphylla color starts jaundice, starts the moulding in 20-30 minute that stir-fries of little fire.The gynostemma pentaphylla of having fried is put into fragrance extracting machine Titian 30-45 minute, after taking out, naturally dry.

Get again other raw material Radix Angelicae Sinensis 1500g, red sage root 1500g, plantain seed 1500g, reticulate millettia 1500g, rhizoma alismatis 1500g, Poria cocos 1500g, levisticum 1500g, cortex acanthopanacis 1500g, Radix Astragali 1500g, Ligusticum wallichii 1500g, root of Chinese trichosanthes 1500g, root of large-flowered skullcap 1500g, bighead atractylodes rhizome 1500g, lotus leaf 1500g, in the ethanol that adds 5-10 doubly to measure, soak 1-2 hour, heating is extracted 2 times, each 1-2 hour, remove supernatant, merge extract, 100-120 order filters, through the molecular weight that dams, be the ultrafiltration post ultrafiltration of 5000-10000 again, 1.36 medicinal extract when ultrafiltrate reduced pressure concentration relative density is 80 ℃, heating is concentrated into paste, stand for standby use, become component 2.Component 1 and component 2 mixed and inserted in economic benefits and social benefits vacuum concentrator, the concentrate that while being concentrated into 90 ℃, relative density is 1.05, set to 0～5 ℃ of deepfreezes 24 hours; Refrigeration liquid is added to 0.3% filter aid diatomite, filter, filtrate is inserted in economic benefits and social benefits vacuum concentrator again, is concentrated into every 1ml containing 1g crude drug amount, and then add honey and be in harmonious proportion, granulation agent, the gynostemma pentaphylla of mixing Titian, ultraviolet disinfection, packs and get final product.

Specific embodiment 3:

The fresh and tender gynostemma pentaphylla 3800g of manual harvesting, airing, parches; The good gynostemma pentaphylla of airing is put into the pot that completes and go green grass or young crops, the 30-45 minute that completes at 70-100 ℃, then puts into large iron pan and stir-fries, first downwards backward upper, firmly wants evenly, when gynostemma pentaphylla color starts jaundice, starts the moulding in 20-30 minute that stir-fries of little fire.The gynostemma pentaphylla of having fried is put into fragrance extracting machine Titian 30-45 minute, after taking out, naturally dry and get other raw material Radix Angelicae Sinensis 1300g, red sage root 1200g, plantain seed 1100g, reticulate millettia 1200g, rhizoma alismatis 1100g, Poria cocos 1300g, levisticum 1200g, cortex acanthopanacis 1100g, Radix Astragali 1200g, Ligusticum wallichii 1300g, root of Chinese trichosanthes 1100g, root of large-flowered skullcap 1100g, bighead atractylodes rhizome 1100g, lotus leaf 1200g, gets bulk drug and cleans and to dry, in 70 ℃ of baking ovens, dry 8-12h, taking-up is placed in drying box cooling, with pulverizer, pulverizes 80-100 mesh sieve.The particle that sieves is soaked in water 12 hours, then put into extractor heating and boil 1 hour, filter filtrate for later use; Filter residue adds water, and heating, boils 45 minutes for the second time, filters filtrate for later use; Filter residue adds water again, and heating is for the third time boiled half an hour, filters; Three filtrates are combined, powder is placed in 70-90% ethanol and soaks 1-2 hour, is heated to 60 ℃-70 ℃, lixiviate 2h in water-bath, every 10min stirs once, by filtered through gauze, in residue, add 50-60% ethanol, 60 ℃-70 ℃ are continued lixiviate 2h, every 10min stirs once, filtered through gauze, merges leaching liquor, is condensed into pasty state.Then add honey and be in harmonious proportion, granulation agent, the gynostemma pentaphylla of mixing Titian, ultraviolet disinfection, packs and get final product.

Pharmacology toxicity test

One, skin irritation test

1, experimental technique

Guinea pig back backbone both sides are evenly coated hair remover, make approximately 20 square centimeters of unhairing scopes.Clean hair remover returns cage to observe 24 hours, and every group of cavy is coated with respectively the present invention 4,8 and 12g, and respectively containing crude drug 92mg, 184mg and 368mg, another group is coated with the every twice-daily of solvent 0.8ml, continuous one week, after experiment finishes, cavy is put to death, the heart, liver, kidney and depilation skin are done pathological examination.

2, result

Above-mentioned three groups of medication cavy trunks depilation district, having no local skin has oedema, hyperemia, erythema, blutpunkte and ulcer.Medication group cavy chroma of hair, ingest, the control group no significant difference such as four limbs activity, histopathologic examination, the administration group heart, liver, kidney and depilation skin with contrast more also no significant difference of word.

Results suggest, the present invention, without local irritant effect, also has no general toxicity performance.Show that preparation of the present invention is used safety.

Two, animal acute toxicity test research:

The present invention, through trial test, can not measure LD50, therefore measure maximum dosage-feeding.Adopt rectally approach, the maximum dosage-feeding on the one that records mouse is 62.7g/kg (in medicinal powder), be about 1567.5 times (people is in 60kg) intending clinical people's consumption, the maximum dosage-feeding 62.7g/kg (in medicinal powder) on the one of rat, is about and intends 1567.5 times of clinical people's consumption.The equal Continuous Observation of animal used as test one week, its outward appearance sign, the state of mind, diet, drinking-water, ight soil etc. are showed no obvious abnormalities.When experiment finishes, animal is healthy survival all, and body weight increases.

Three, long-term toxicity test for animals research:

It is high that medicine of the present invention is established in this test, in, low dose group and matrix control group, blank group, adopt rectally approach to give rat, dosage is respectively 3.2, 2.4 and 1.6g/kg (in medicinal powder), be equivalent to intend 80 of clinical patient consumption [0.04g (medicinal powder)/kg], 60 and 40 times, successive administration 2 months, in administration process, the body weight of each administration group and matrix group rat, dietary amount, outward appearance sign, behavioral activity, the state of mind, hair color, ight soil proterties and the comparison of blank group, no significant difference (P > 0.05), the weight of animals increasing value and Main Organ Coefficients and blank group comparing difference be remarkable (P > 0.05) not, hematology and the indivedual indexs of blood parameters check result administration group and blank group comparing difference be (P < 0.05) significantly, but convalescence finishes rear discrepant hematology and blood parameters has all recovered normal, other indexs and blank comparing difference be remarkable (P > 0.05) not, administration phase and convalescence histological examination high dose group animal main organs and blank group relatively have no notable difference, having no slowness toxicity occurs.

Pharmacological experiment:

Along with industrialized development, the variation of dietary structure, the improvement of living condition, Overweight and obesity crowd's quantity worldwide increases rapidly, and be the trend of becoming younger, become the global healthy epidemic disease that has a strong impact on, therefore, the effective method of weight-reducing of search of health has very real significance, and the active material of losing weight is safely and effectively the key that people expect to solve problem of obesity always.

1 materials and methods

1.1 materials and instrument

Experimental rat is fed high lipid food standard formula full price mouse feed 60%, lard 14%, sucrose 5%, milk powder 5%, peanut 5%, egg 8%, sesame oil 1%, salt 2% used, by Animal Science Co., Ltd of Shandong University, is processed; Other agents useful for same are that commercially available analysis is pure; T-CHOL detection kit, triglycerides detection kit and the firelight or sunlight biological Co., Ltd of HDL detection kit wound; Just the male SD rat 100Zhi You Shandong University Experimental Animal Center of ablactation provides, and raises that [temperature (25 ± 2) ℃, relative humidity is 50% in SPF level zoopery center, this school.BP211D type electronic analytical balance Germany Satrorius company; 202 type Constant Temp. Oven Shanghai Suo Pu Instrument Ltd.; 1-15PK small desk refrigerated centrifuge Germany Sigma company; TU-1800S ultraviolet-uisible spectrophotometer Beijing Puxi General Instrument Co., Ltd; PUZS-300 full automatic biochemical apparatus Nanjing Pu Lang medical equipment Co., Ltd; Stainless steel metabolism of rat cage Beijing good Asource industry Science and Technology Ltd.; SOX406 Milko-Tester Jinan Hai Neng Instrument Ltd..

1.2 the present invention feed high fat are fed to the impact that rat body weight increases

1.2.1 the male Wistar rat that animal used as test and grouping are grown 3 months, body weight (220 ± 10) g, 50 of sums, are provided by Shandong University's Experimental Animal Center, raise in SPF level zoopery center, this school [temperature (25 ± 2) ℃, relative humidity is 50%].By body weight, be divided at random 5 groups, 10 every group.Animal grouping is as follows with administration situation:

Blank group (Blank): gavage etc. are held physiological saline, continuous 9 weeks; Gynostemma pentaphylla group (CH): 400mg/kg/d, gavage is 9 weeks continuously; Other raw material group (CU): 400mg/kg/d, gavage is 9 weeks continuously; Low dose group of the present invention (CH+CU-low): 400mg/kg/d, gavage is 9 weeks continuously; High dose group of the present invention (CH+CU-high): 800mg/kg/d, gavage is 9 weeks continuously.

1.2.2 observation index and detection method accurately weigh the initial body weight of rat, are distributed into cage, and 1, every cage guarantees the abundant supply of water and food, measures weekly the intake of food and water, measures increased weight weekly simultaneously.Blood 0.5mL is got in the 1st week and the 9th week regularly eye socket, and immediately in 2500r/min refrigerated centrifuge 2min, automatic clinical chemistry analyzer detects total sterol, high-density protein and the content of triglyceride in serum.Experiment in the 9th week finishes front 1d, and rat is put into metabolic cage, collects voiding excreta of 24h animal, measures ight soil gross weight, gets quantitative dry sample ight soil, and Soxhlet extraction process is measured the excrement fat content in ight soil, calculates when excrement fat weight ratio of excrement body weight simultaneously.

1.3 the present invention feed the impact on obese rat fat-reducing

1.3.1 eating 10 of source property obese rat modeling methods is standard full price mouse forage feed, feeds for 90 with high lipid food.Feed after 3 months, it is obesity standard that the body weight of take surpasses 20% of normal forage feed rat body weight mean value, 49 of modeling successes, and success rate is 54.4%.

1.3.2 grouping and the successful obese rat of processing method modeling change to the nursing of standard complete feed subsequently, are divided at random 5 groups, 9 every group by body weight.Blank group (Blank): model control rats, gavage equal solvent physiological saline, continuous 4 weeks; Gynostemma pentaphylla group (CH): 400mg/kg/d, gavage is treated 4 weeks; Other raw material group (CU): 400mg/kg/d, gavage is treated 4 weeks; Low dose group of the present invention (CH+CU-low): 400mg/kg/d, gavage is treated 4 weeks; High dose group of the present invention (CH+CU-high): 800mg/kg/d, gavage is treated 4 weeks.

1.3.3 observation index and detection method weigh body weight before and after treatment with electronic scale, calculate body weight decrement before and after administration, and the body weight decrement of usining accounts for the percentage of body weight before administration as weight-loss ratio.Experiment finishes the previous day, and rat eye socket is got to blood 2mL, and immediately in 2500r/min refrigerated centrifuge 2min, automatic clinical chemistry analyzer detects total sterol, the high density lipoprotein level bletilla content of triglyceride in serum.Experiment finishes rear execution rat, gets lower rear fat of omoplate (brown fat) and weighs; Get all Abdominal Wall Fats of kidney and epididymal adipose tissues (white adipose), with both sums, represent visceral fat mass; Calculate interior fat weight ratio and a rear fat-body anharmonic ratio simultaneously.

1.4 data statistic analysis

With SPSS100 software, carry out data statistic analysis, adopt Homogeneity-of-variance to carry out homogeneity test of variance, if variance is neat, carry out one-way analysis of variance, and adopt Dunnett-test to carry out comparing between two between experimental group and control group mean; If heterogeneity of variance, does nonparametric statistics with Kruskal-Wallistest, with Mann-Whimeytest, carry out comparing between two between mean.

2 results and analysis

2.1 the present invention feed high fat are fed to the impact that rat body weight increases: in whole experimentation, without rats death, subordinate act and figure observe each experimental group animal without significantly taking bad reaction.Specific experiment result as shown in Table 1 to Table 3.

Table 1 animal subject food ration, amount of drinking water and increased weight weekly changes (n=10, X ± SD)

Note: represent there was no significant difference (p > 0.05) with column data acceptance of the bid note same letter; Difference has significant difference (p < 0.05); Table 2～table 5 is same.

As shown in Table 1, each experimental group drinking water for animals amount and food ration are substantially suitable, illustrate that gavage gives each active component and do not affect rat and ingest and freely drink water, and each activity and digestive system function of organizing animal used as test is normal.With this understanding, with blank group rat, compare, become slowly to the rat body weight increase of gynostemma pentaphylla and other raw materials, but not obvious (p > 0.05); And the composition that gives high and low dose of the present invention can comparatively significantly suppress the body weight increase of rat, when respectively at the 3rd, 4 weeks, there is (p < 0.05) in depression effect, and extend in time and strengthen, the weight of animals increase that gives high dose composition suppresses more obvious.

The body weight increase of blank group rat is due to the feed that has absorbed the high sugar of high fat, after superfluous nutritional labeling is taken in, through absorbing, enters in body, pass through again transhipment, metabolism and conversion, savings, in adipocyte, and are accumulated in different Organ and tissues, cause fat.Though and give curcumin and shitosan does not reduce food ration, but can play a role forming other fat processes.The effect of gynostemma pentaphylla is in animal gastrointestinal tract, pass through absorption and in conjunction with a large amount of fat, form ight soil and excrete, thereby the relative uptake of fat is reduced, and suppresses fat generation; It also has an effect is after fat absorption, to pass through to promote fat metabolism, thereby falls relative accumulation in low-fat body, thereby suppresses obesity.

The blood lipid level of table 2 animal subject changes (n=10, X ± SD)

As shown in Table 2, while testing 1 week, each organizes the horizontal no significant difference (p > 0.05) of T-CHOL, triglycerides and the HDL of rat; After gavage 9 weeks, and the triglycerides and the hdl level that give the experimental rat of composition have certain decline, but trend not obvious (p > 0.05).Animal used as test, in high fat nursing process, although given various administration interventions, still has corresponding increase at the blood lipid level of processing early stage (1 week) and later stage (9 weeks).With each treated animal, by the result that the blood lipid level of feeding 9 weeks deducts the blood lipid level of nursing in the time of 1 week, represent blood lipid level increment, discovery gives the blood lipid level increment that gynostemma pentaphylla or other raw materials or both composition the present invention all can reduce rat in high fat nursing process, wherein of the present invention group of blood lipid level increment is minimum, effect is best, illustrates that giving the present invention can effectively suppress the blood lipid level increase that high fat is fed rat.

The situation (n=10, X ± SD) of table 3 animal subject fat excretion

Table 3 result shows, high and low dose of the present invention all can promote the defecation of rat to a certain extent, but defecation amount is without obvious difference (p > 0.05), but excrement fat amount and excrement fat weight ratio have obvious increase (p < 0.05), illustrate and give the effect that above-mentioned substance can play fat consumption, give other raw material groups without this effect.This is because gynostemma pentaphylla wherein has the function of stronger absorbing fats in vivo, can promote fatty consumption, thereby play good fat-reducing effect.

2.2 curcumins and shitosan nursing are to each experimental group animal in the whole experimentation of the fat-reducing effect of obese rat without obviously taking bad reaction, and specific experiment result is as shown in table 4～table 5.

Table 4 is respectively organized the relevant body weight parameter (n=9, X ± SD) of tested obese rat

As shown in Table 4, after modeling success, the body weight average of obese rat surpasses 630g, give active material gavage after 4 weeks, the body weight of gynostemma pentaphylla group, present composition group obese rat has conspicuousness to alleviate (p < 0.05) compared with control group, other raw material groups also have certain phenomenon that loses weight, but compare not obvious (p > 0.05) with blank group, the effect of composition is better than independent use gynostemma pentaphylla and other raw materials (p < 0.05), and present certain dose-effect relationship, high dose better effects if.

Table 5 is respectively organized the different fat sites content (n=9, X ± SD) of tested obese rat

Group

Interior fat (g)

Internal organ fat body ratio

Fat (g) after

Fat body ratio after

[0096]?

?	?	(％)	?	(‰)
					Blank	17.61±1.77a	2.82±0.23a	0.52±0.01a	9.32±0.31a
CH	12.27±1.22b	2.16±0.21b	0.51±0.04a	9.43±0.92a
					CU	11.92±0.98b	2.14±0.15b	0.49±0.06a	9.32±1.27a
CH+CU-is low	11.42±1.31b	2.12±0.12b	0.48±0.04a	9.16±0.81a
					CH+CU-is high	10.68±2.08b	1.91±0.31b	0.36±0.02b	5.84±0.31b

As shown in Table 5, with blank group, compare, alone gynostemma pentaphylla and other raw materials can reduce the visceral adipose of obese rat and internal organ fat body than (p < 0.05), the present invention has the effect (p < 0.05) of obvious reduction obese rat interior fat equally, its effect is even better than alone group of gynostemma pentaphylla or other raw materials, present composition high dose more remarkable effect, so that also show a rear fat of obvious reduction obese rat and an effect (p < 0.05) for a rear fat body ratio.

Discuss: this research has verified that in whole animal level gynostemma pentaphylla has good inhibition body weight increase and fat-reducing effect, and after gynostemma pentaphylla and other raw materials of the present invention are used in conjunction with, both have played the effect of Synergistic.

What the method for weight-reducing of mistake was brought can only be wrong result.How should we subtract so? find out self fat root, suit the remedy to the case.Have lipodystrophy, good, we offer its sufficient nutrition, activate it, otherwise the heat or eat slimming drugs of cutting down one's diet simply, only can make this obstacle make the matter worse, your oil supply not of machine, light is want to rotate it by external force, and result well imagines, people's cell too.Of excessive eating and drinking, take in heat too high, good, we must control the diet of controlling oneself, and low-keyed eating, selectively eats, and free eats, rather than suffering from hunger simply.

Simply suffer from hunger and can manage it, fat-reducing just can not become international a difficult problem, and everybody comes to starve upper several, and all things is all stopped.Consume too low cause eat like a bird also fat? alright, manage to increase basal metabolic rate, manage to increase activity.Does disease cause obesity? alright, go to hospital, what all need not be managed, and first cures the disease and loses weight afterwards at once.Fat reason emphasis is different, wants to lose weight and please first finds the problem of oneself accurately.

Claims

1. a gynostemma pentaphylla lowering blood-fat and reducing weight health protection tea, is characterized in that, in described gynostemma pentaphylla lowering blood-fat and reducing weight health protection tea, the parts by weight of various raw materials comprise: 10～50 parts of gynostemma pentaphylla, 10～20 parts of Radix Angelicae Sinensis, 10～20 parts of the reds sage root, 10～20 parts of plantain seeds, 10～20 parts of reticulate millettias, 10～20 parts of rhizoma alismatis, 10～20 parts, Poria cocos, 10～20 parts of levisticums, 10～20 parts of cortex acanthopanacis, 10～20 parts of the Radixs Astragali, 10～20 parts of Ligusticum wallichiis, 10～20 parts of roots of Chinese trichosanthes, 10～20 parts of the roots of large-flowered skullcap, 10～20 parts of the bighead atractylodes rhizomes, 10～20 parts, lotus leaf.

2. gynostemma pentaphylla lowering blood-fat and reducing weight health protection tea according to claim 1, it is characterized in that, in described gynostemma pentaphylla lowering blood-fat and reducing weight health protection tea, the parts by weight of various raw materials comprise: 10～30 parts of gynostemma pentaphylla, 10～15 parts of Radix Angelicae Sinensis, 10～15 parts of the reds sage root, 10～15 parts of plantain seeds, 10～15 parts of reticulate millettias, 10～15 parts of rhizoma alismatis, 10～15 parts, Poria cocos, 10～15 parts of levisticums, 10～20 parts of cortex acanthopanacis, 10～20 parts of the Radixs Astragali, 10～20 parts of Ligusticum wallichiis, 10～20 parts of roots of Chinese trichosanthes, 10～20 parts of the roots of large-flowered skullcap, 10～20 parts of the bighead atractylodes rhizomes, 10～20 parts, lotus leaf.

3. gynostemma pentaphylla lowering blood-fat and reducing weight health protection tea according to claim 1, it is characterized in that, in described gynostemma pentaphylla lowering blood-fat and reducing weight health protection tea, the parts by weight of various raw materials comprise: 10～40 parts of gynostemma pentaphylla, 10～20 parts of Radix Angelicae Sinensis, 10～20 parts of the reds sage root, 10～20 parts of plantain seeds, 10～20 parts of reticulate millettias, 10～20 parts of rhizoma alismatis, 10～20 parts, Poria cocos, 10～20 parts of levisticums, 10～15 parts of cortex acanthopanacis, 10～15 parts of the Radixs Astragali, 10～15 parts of Ligusticum wallichiis, 10～15 parts of roots of Chinese trichosanthes, 10～15 parts of the roots of large-flowered skullcap, 10～15 parts of the bighead atractylodes rhizomes, 10～15 parts, lotus leaf.

4. a preparation method for gynostemma pentaphylla lowering blood-fat and reducing weight health protection tea as described in claim 1～3 any one, is characterized in that, the preparation process of described gynostemma pentaphylla lowering blood-fat and reducing weight health protection tea comprises following aspect:

A. the preparation of gynostemma pentaphylla;

B. the gynostemma pentaphylla Titian of having fried;

C. described other raw material soaking ethanol heating are extracted;

E. concentrated granule, pulvis or paste are mixed with the gynostemma pentaphylla preparing, sterilizing is prepared into health care medicinal tea.

5. the preparation method of gynostemma pentaphylla lowering blood-fat and reducing weight health protection tea according to claim 4, is characterized in that, in described step a, plucks fresh and tender gynostemma pentaphylla by hand, and airing, parches; The good gynostemma pentaphylla of airing is put into the pot that completes and go green grass or young crops, the 30-45 minute that completes at 70-100 ℃, then puts into large iron pan and stir-fries, first downwards backward upper, firmly wants evenly, when gynostemma pentaphylla color starts jaundice, starts the moulding in 20-30 minute that stir-fries of little fire.

6. the preparation method of gynostemma pentaphylla lowering blood-fat and reducing weight health protection tea according to claim 4, is characterized in that, in described step b, the gynostemma pentaphylla of having fried is put into fragrance extracting machine Titian 30-45 minute, after taking out, naturally dries.

7. the preparation method of gynostemma pentaphylla lowering blood-fat and reducing weight health protection tea according to claim 4, is characterized in that, in described step c, get in the ethanol that other raw materials add 5-10 doubly to measure and soak 1-2 hour, heating is extracted 2 times, and each 1-2 hour, removes supernatant, merge extract, 100-120 order filters, then is the ultrafiltration post ultrafiltration of 5000-10000 through the molecular weight that dams, 1.36 medicinal extract when ultrafiltrate reduced pressure concentration relative density is 80 ℃, heating is concentrated into paste, stand for standby use.

8. the preparation method of gynostemma pentaphylla lowering blood-fat and reducing weight health protection tea according to claim 4, it is characterized in that, in described steps d, concentrated medicinal extract is inserted in economic benefits and social benefits vacuum concentrator, the concentrate that while being concentrated into 90 ℃, relative density is 1.05, set to 0～5 ℃ of deepfreezes 24 hours; Refrigeration liquid is added to 0.3% filter aid diatomite, filter, filtrate is inserted in economic benefits and social benefits vacuum concentrator again, is concentrated into every 1ml containing 1g crude drug amount; Paste after concentrated adds the agent of honey mediation granulation; Or convection drying is made pulvis.

9. the preparation method of gynostemma pentaphylla lowering blood-fat and reducing weight health protection tea according to claim 4, is characterized in that, in described step e, the gynostemma pentaphylla making is mixed with other raw materials of refinement, after ultraviolet ray sterilization bactericidal, packs.

10. the preparation method of a gynostemma pentaphylla lowering blood-fat and reducing weight health protection tea as described in any one in claim 1～3, it is characterized in that, its preparation process can also be: get bulk drug and clean and to dry, in 70 ℃ of baking ovens, dry 8-12h, taking-up is placed in drying box cooling, with pulverizer, pulverizes 80-100 mesh sieve; The particle that sieves is soaked in water 12 hours, then put into extractor heating and boil 1 hour, filter filtrate for later use; Filter residue adds water, and heating, boils 45 minutes for the second time, filters filtrate for later use; Filter residue adds water again, and heating is for the third time boiled half an hour, filters; Three filtrates are combined, and powder is placed in 70-90% ethanol and soaks 1-2 hour, is heated to 60 ℃-70 ℃ in water-bath, lixiviate 2h, every 10min stirs once, by filtered through gauze, in residue, add 50-60% ethanol, 60 ℃-70 ℃ are continued lixiviate 2h, and every 10min stirs once, filtered through gauze, merge leaching liquor, be condensed into pasty state, by pasty state extract mediation honey or dextrin, granulation agent; Or convection drying is made pulvis.