CN103519024A - Method for manufacturing feed additive for feeding black-bone chicken - Google Patents

Method for manufacturing feed additive for feeding black-bone chicken Download PDF

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Publication number
CN103519024A
CN103519024A CN201310510737.3A CN201310510737A CN103519024A CN 103519024 A CN103519024 A CN 103519024A CN 201310510737 A CN201310510737 A CN 201310510737A CN 103519024 A CN103519024 A CN 103519024A
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CN
China
Prior art keywords
black
bone chicken
aureobasidium pullulans
nutrient solution
feed additive
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201310510737.3A
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Chinese (zh)
Inventor
赵云财
吕伟民
李娜
宋殿峰
张劲松
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
HEILONGJIANG-PROV ACADEMY OF LIGHT INDUSTRY SCIENCES
Original Assignee
HEILONGJIANG-PROV ACADEMY OF LIGHT INDUSTRY SCIENCES
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Application filed by HEILONGJIANG-PROV ACADEMY OF LIGHT INDUSTRY SCIENCES filed Critical HEILONGJIANG-PROV ACADEMY OF LIGHT INDUSTRY SCIENCES
Priority to CN201310510737.3A priority Critical patent/CN103519024A/en
Publication of CN103519024A publication Critical patent/CN103519024A/en
Pending legal-status Critical Current

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  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Fodder In General (AREA)

Abstract

The invention relates to a method for manufacturing feed additives, and in particular relates to a method for manufacturing a feed additive for feeding black-bone chicken. The method comprises the steps of preparing an aureobasidium pullulan culture solution, heating and concentrating the aureobasidium pullulan culture solution to 50Bx, uniformly mixing the concentrated culture solution with bran of the same mass, drying till the water content is below 10%, smashing, and screening by a 20-mesh sieve, thus obtaining the finished product. The feed additive disclosed by the invention contains melanin produced in growth and reproduction processes of aureobasidium pullulans, the immunity and the disease resistance of the black-bone chicken eating the feed additive can be increased and the internal melanin content of the black-bone chicken can also be increased, so that the medicinal value and the edible value of the black-bone chicken are increased.

Description

Feed the preparation method of feed addictive for black-bone chicken
Technical field
The present invention relates to the preparation method of feed addictive, particularly a kind of preparation method of feeding black-bone chicken use feed addictive.
Background technology
Black-bone chicken and the most obvious difference of common chicken are that body contains a large amount of melanin.The effect of being used as medicine of black-bone chicken is relevant with the melanin depth in black-bone chicken skin, bone, meat, and the darker person of its color, is used as medicine better.First melanic effect is to unrighted acid Wheat Protein, secondly melanin remove in addition free radical function, be antidotal effect.Modern medicine also thinks that the medical value of black-bone chicken and edibility are present in body in melanin material, often eats black-bone chicken and can play nourishing, improves the health, anti-ageingly prevent the effect of waiting for a long time.But in the process of raising black-bone chicken, in order to deepen the color of black-bone chicken skin, bone, meat, the phenomenon of abusing and abuse feed medicated premix is more serious, causes medicine residual in a large number in chicken body, cause medical value and the tonic effect of chicken to decline, even affect the health of human body.
Summary of the invention
The object of this invention is to provide a kind of preparation method safe, that increase the feed addictive of black-bone chicken skin, bone, meat melanin content.
The object of the present invention is achieved like this:
(1) preparation of Aureobasidium pullulans nutrient solution:
Remove skin potato and be cut into small pieces, add the water boil 30 minutes of 5 times of quality, elimination potato ball, complements to original volume by filtrate, obtains potato immersion liquid.In potato immersion liquid, add 2.5% glucose, 0.2% beef extract, 0.2% casein peptone, 0.1% potassium dihydrogen phosphate, 0.05% magnesium sulfate, 0.05% sodium chloride, 0.05% tyrosine, 0.002% copper chloride, PH is adjusted to 5.8-6.2, at 121 ℃, sterilizing is 20 minutes, be cooled to 30 ℃, inoculation Aureobasidium pullulans, under 29~31 ℃, the condition of rotary shaker 180rpm, cultivate 5 days, obtain Aureobasidium pullulans nutrient solution.
(2) feed the making of feed addictive for black-bone chicken:
Aureobasidium pullulans nutrient solution heating is concentrated into 50Bx, with etc. the wheat bran of quality mix, be then dried to moisturely below 10%, pulverized 20 mesh sieve and got product.
Feed addictive of the present invention, contain the melanin producing in Aureobasidium pullulans growth and breeding process, after black-bone chicken is edible, can not only increase its immunity, improve resistance against diseases, can also increase the melanin content in its body, improve medical value and the edibility of black-bone chicken.While feeding black-bone chicken, feed addictive of the present invention is joined in silky fowl feed and mixed in the ratio of 1-2%.
(4), specific embodiments
The bacterial strain that the present invention adopts is Aureobasidium pullulans As3.933.
(1) preparation of Aureobasidium pullulans nutrient solution:
Remove 200 grams of skin potatos, be cut into small pieces, add 1000ml water boil 30 minutes, elimination potato ball, complements to 1000 milliliters by filtrate, obtains potato immersion liquid.In potato immersion liquid, add 2.5% glucose, 0.2% beef extract, 0.2% casein peptone, 0.1% potassium dihydrogen phosphate, 0.05% magnesium sulfate, 0.05% sodium chloride, 0.05% tyrosine, 0.002% copper chloride, PH is adjusted to 6.0, at 121 ℃, sterilizing is 20 minutes, be cooled to 30 ℃, inoculation Aureobasidium pullulans is cultivated 5 days under 30 ℃, the condition of rotary shaker 180rpm.
(2) feed the making of feed addictive for black-bone chicken:
Aureobasidium pullulans nutrient solution heating is concentrated into 50Bx, with etc. the wheat bran of quality mix, be then dried to moisturely below 10%, pulverized 20 mesh sieve and got product.

Claims (2)

1. feed the preparation method of feed addictive for black-bone chicken, it is characterized in that: first prepare Aureobasidium pullulans nutrient solution, Aureobasidium pullulans nutrient solution heating be concentrated into 50Bx, with etc. the wheat bran of quality mix, then be dried to moisturely below 10%, pulverized 20 mesh sieve and get product.
2. the preparation method of feed addictive for nursing according to claim 1 black-bone chicken, it is characterized in that: described Aureobasidium pullulans nutrient solution preparation method is: remove skin potato and be cut into small pieces, the water boil 30 minutes that adds 5 times of quality, elimination potato ball, filtrate is complemented to original volume, obtain potato immersion liquid, in potato immersion liquid, add 2.5% glucose, 0.2% beef extract, 0.2% casein peptone, 0.1% potassium dihydrogen phosphate, 0.05% magnesium sulfate, 0.05% sodium chloride, 0.05% tyrosine, 0.002% copper chloride, PH is adjusted to 5.8-6.2, at 121 ℃, sterilizing is 20 minutes, be cooled to 30 ℃, inoculation Aureobasidium pullulans, at 29~31 ℃, under the condition of rotary shaker 180rpm, cultivate 5 days, obtain Aureobasidium pullulans nutrient solution.
CN201310510737.3A 2013-10-27 2013-10-27 Method for manufacturing feed additive for feeding black-bone chicken Pending CN103519024A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201310510737.3A CN103519024A (en) 2013-10-27 2013-10-27 Method for manufacturing feed additive for feeding black-bone chicken

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201310510737.3A CN103519024A (en) 2013-10-27 2013-10-27 Method for manufacturing feed additive for feeding black-bone chicken

Publications (1)

Publication Number Publication Date
CN103519024A true CN103519024A (en) 2014-01-22

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109717327A (en) * 2019-03-19 2019-05-07 广西大学 Feed for black bone chickens additive and the preparation method and application thereof
CN109876062A (en) * 2019-03-19 2019-06-14 广西大学 Facilitate the Chinese medicine powder and the preparation method and application thereof of melanin of black-bone chicken deposition

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1817178A (en) * 2006-03-23 2006-08-16 江西汇仁药业有限公司 Use of forage containing germanium for cock
CN101569350A (en) * 2008-05-02 2009-11-04 王广清 Preparation method of black-bone chicken feed
CN102229754A (en) * 2011-05-12 2011-11-02 华中农业大学 Method for extracting melanin from waste tremella cultivating material
CN102517335A (en) * 2011-12-16 2012-06-27 天津北洋百川生物技术有限公司 Method for producing novel natural melanin
CN102940146A (en) * 2012-11-30 2013-02-27 江西省农业科学院畜牧兽医研究所 Chinese herbal medicine feed additive for Taihe black-boned chickens

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1817178A (en) * 2006-03-23 2006-08-16 江西汇仁药业有限公司 Use of forage containing germanium for cock
CN101569350A (en) * 2008-05-02 2009-11-04 王广清 Preparation method of black-bone chicken feed
CN102229754A (en) * 2011-05-12 2011-11-02 华中农业大学 Method for extracting melanin from waste tremella cultivating material
CN102517335A (en) * 2011-12-16 2012-06-27 天津北洋百川生物技术有限公司 Method for producing novel natural melanin
CN102940146A (en) * 2012-11-30 2013-02-27 江西省农业科学院畜牧兽医研究所 Chinese herbal medicine feed additive for Taihe black-boned chickens

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109717327A (en) * 2019-03-19 2019-05-07 广西大学 Feed for black bone chickens additive and the preparation method and application thereof
CN109876062A (en) * 2019-03-19 2019-06-14 广西大学 Facilitate the Chinese medicine powder and the preparation method and application thereof of melanin of black-bone chicken deposition
CN109876062B (en) * 2019-03-19 2021-07-27 广西大学 Traditional Chinese medicine powder beneficial to melanin deposition of black-bone chicken and preparation method and application thereof
CN109717327B (en) * 2019-03-19 2021-07-27 广西大学 Black-bone chicken feed additive and preparation method and application thereof

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Application publication date: 20140122