CN109876062B - Traditional Chinese medicine powder beneficial to melanin deposition of black-bone chicken and preparation method and application thereof - Google Patents
Traditional Chinese medicine powder beneficial to melanin deposition of black-bone chicken and preparation method and application thereof Download PDFInfo
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Abstract
The invention discloses a traditional Chinese medicine powder beneficial to melanin deposition of black-bone chickens, which is mainly prepared from the following raw materials in parts by weight: 35-40 parts of eupolyphaga, 25-30 parts of elephant, 25-30 parts of medicinal grain steatite, 25-30 parts of saw grain steatite, 25-30 parts of lumbricus, 15-20 parts of glossy privet fruit, 15-20 parts of crocus sativus, 7-15 parts of phoenix tree seed, 7-15 parts of ligusticum chuanxiong hort, 3-6 parts of salvia miltiorrhiza, 3-6 parts of poria cocos and 3-6 parts of seaweed. The invention discloses a preparation method of traditional Chinese medicine powder, which comprises the steps of crushing eupolyphaga, earthworm, glossy privet fruit, crocus sativus, phoenix tree seed and the like, mixing to obtain a mixture, and extracting the mixture by an alcohol extraction method. The invention discloses application of traditional Chinese medicine powder in improving melanin deposition of Lingyun black-bone chicken cocks. The invention can improve the content of copper, tyrosine and vitamin B in the Chinese medicinal powder6The content of the black-bone chicken in the black-bone chicken cock is improved.
Description
Technical Field
The invention relates to the field of feed additives. More specifically, the invention relates to a traditional Chinese medicine powder beneficial to melanin deposition of black-bone chickens, and a preparation method and application thereof.
Background
Lingyun black-bone chicken is named as Guangxi Lingyun county, and the beak, the crown, the tibiodigital, the skin, the meat and the internal organs are all black, so local masses are commonly called as 'Wuwu black-bone chicken'. The variety has the characteristics of coarse feeding resistance, foraging capability, beautiful feather, tender meat quality, delicious and strong flavor, high edible and medicinal values and the like, and is a delicacy and nourishing good item on a dining table. The black-bone chicken is an important characteristic medicinal and edible resource in China, and compared with non-black-bone chicken, the black-bone chicken has the most obvious characteristic that the black-bone chicken is rich in melanin in vivo, and the melanin is an important material basis for the black-bone chicken to exert the medicinal effect. For a long time, people use black-bone chicken as a traditional Chinese medicine and a good tonic product, and modern researches find that black-bone chicken melanin has the functions of resisting fatigue, oxidation and mutation, preventing radiation, delaying aging, improving the immunity of an organism, removing lead, detoxifying, eliminating free radicals and the like, is an important active ingredient in the organism and is a material basis of the active function, and the melanin determines the medicinal value of the black-bone chicken. The black-bone chicken with higher melanin content in body has higher medicinal nourishing value. Besides being related to chicken genetic factors, the accumulation of melanin in the black-bone chicken can also affect the formation of black-bone chicken melanin by non-genetic factors, such as feed, diseases and the like. Meanwhile, multiple times of breeding show that the black degree of the Lingyun black-bone chicken cock is lower than that of the Lingyun black-bone chicken, no matter the black degree of the Lingyun black-bone chicken is skin, internal organs and bones.
Disclosure of Invention
An object of the present invention is to solve at least the above problems and to provide at least the advantages described later.
Still another object of the present invention is to provide a Chinese medicinal powder for melanin deposition in black-bone chicken, which is helpful for melanin deposition in Lingyun black-bone chicken, and a method for preparing the Chinese medicinal powder for melanin deposition in black-bone chicken, which can improve the content of copper, tyrosine and vitamin B in the Chinese medicinal powder6Content of (A) to (B) to provide a Chinese medicinal powderThe application of the black pigment in improving the melanin deposition of the Lingyun black-bone chicken cock is disclosed.
To achieve these objects and other advantages in accordance with the present invention, there is provided a Chinese medicinal powder for promoting melanin deposition in black-bone chickens, which is prepared from the following raw materials in parts by weight: 35-40 parts of eupolyphaga, 25-30 parts of elephant, 25-30 parts of medicinal grain steatite, 25-30 parts of saw grain steatite, 25-30 parts of lumbricus, 15-20 parts of glossy privet fruit, 15-20 parts of crocus sativus, 7-15 parts of phoenix tree seed, 7-15 parts of ligusticum chuanxiong hort, 3-6 parts of salvia miltiorrhiza, 3-6 parts of poria cocos and 3-6 parts of seaweed.
A preparation method of traditional Chinese medicine powder helpful for melanin deposition of black-bone chicken is provided, which comprises pulverizing Eupolyphaga Seu Steleophaga, rice weevil, medicinal Guarar, saw Guarar, Lumbricus, fructus Ligustri Lucidi, stigma croci Sativi, semen Firmianae, rhizoma Ligustici Chuanxiong, Saviae Miltiorrhizae radix, Poria and Sargassum, mixing to obtain a mixture, and extracting the mixture by alcohol extraction to obtain an extract, i.e. traditional Chinese medicine powder.
Preferably, the alcohol extraction method specifically comprises the following steps: adding an ethanol solution with the mass fraction of 95% into the mixture, leaching for 2-4 hours at normal temperature, performing suction filtration to obtain a filtrate A and a filter residue A, performing reduced pressure distillation on the filtrate A, volatilizing to remove ethanol to obtain an ethanol extract, concentrating the ethanol extract to obtain a clear paste with the relative density of 1.15-1.25, uniformly mixing the clear paste with silicon dioxide in a mass ratio of 1:3, and drying until the water content is lower than 5% to obtain the traditional Chinese medicine powder, wherein the mass ratio of the mixture to the ethanol solution with the mass fraction of 95% is 1: 3.
Preferably, before the mixture is extracted by the alcohol extraction method, the mixture is subjected to freeze-thaw treatment, and the specific method comprises the following steps:
stirring the mixture and drinking water according to the mass ratio of 1:1 to form paste, freezing the paste at the temperature of-30 to-20 ℃ for 20-30 h, and then placing the paste at the temperature of 20-30 ℃ until the paste is completely thawed.
Preferably, the mixture has a particle size of less than 100 mesh.
Preferably, before the vacuum distillation of the filtrate a, the method further comprises: adding an ethanol solution with the mass fraction of 95% into the filter residue A according to the mass ratio of 1:3, leaching for 2-4 h at normal temperature, performing suction filtration to obtain a filtrate B and a filter residue B, adding an ethanol solution with the mass fraction of 95% into the filter residue B according to the mass ratio of 1:3, leaching for 2-4 h at normal temperature, performing suction filtration to obtain a filtrate C, and adding the filtrate B and the filtrate C into the filtrate A.
Preferably, the method further comprises pretreating the mixture before freeze-thawing the mixture, and comprises the following specific steps:
adding a solution promoter into the mixture according to the mass ratio of 1:5, stirring uniformly, inserting a positive electrode and a negative electrode, switching on a current with the current intensity of 10mA, electrifying for 50min, and then carrying out ultrasonic treatment for 40min at the ultrasonic frequency of 80kHz and the temperature of 60 ℃, wherein the solution promoter specifically comprises the following components: uniformly mixing salt, sodium citrate, potassium dihydrogen phosphate, pineapple pulp juice and drinking water according to the mass ratio of 0.1:0.2:0.2:1: 2;
wherein, the pineapple pulp is pulped and filtered to obtain juice, and the pineapple pulp juice is obtained.
Provides an application of Chinese medicinal powder in improving melanin deposition of Lingyun black-bone chicken cock.
The invention at least comprises the following beneficial effects: firstly, the method of freeze thawing treatment and three times of alcohol extraction is adopted, so that the copper, the tyrosine and the vitamin B in the traditional Chinese medicine powder can be improved6The content of the Chinese medicinal powder can be further improved, and the function of the Chinese medicinal powder for promoting the melanin deposition of the Lingyun black-bone chicken cock can be further improved. Secondly, the content of phosphorus and polysaccharide in the traditional Chinese medicine powder is improved by combining the action of the solution promoting agent and the current, so that the content of fat in the black-bone chicken is reduced, the content of phospholipid in the black-bone chicken is improved, the content of melanin and phospholipid is increased, the content of fat is reduced, and the nutritional quality of the black-bone chicken is finally improved.
Additional advantages, objects, and features of the invention will be set forth in part in the description which follows and in part will become apparent to those having ordinary skill in the art upon examination of the following or may be learned from practice of the invention.
Detailed Description
The present invention is further described in detail below with reference to examples so that those skilled in the art can practice the invention with reference to the description.
It is to be noted that the experimental methods described in the following embodiments are all conventional methods unless otherwise specified, and the reagents and materials are commercially available unless otherwise specified.
< example 1>
The traditional Chinese medicine powder for helping melanin deposition of the black-bone chicken is mainly prepared from the following raw materials in parts by weight: 35 parts of eupolyphaga, 25 parts of rice weevil, 25 parts of medicinal grain steatite, 25 parts of saw grain steatite, 25 parts of earthworm, 15 parts of glossy privet fruit, 15 parts of crocus sativus, 7 parts of phoenix tree seed, 7 parts of ligusticum chuanxiong hort, 3 parts of salvia miltiorrhiza, 3 parts of poria cocos and 3 parts of seaweed.
Pulverizing Eupolyphaga Seu Steleophaga, Mixiang, medicinal Gusai, saw Gusai, Lumbricus, fructus Ligustri Lucidi, stigma croci Sativi, semen Firmianae, rhizoma Ligustici Chuanxiong, Saviae Miltiorrhizae radix, Poria, and Sargassum, mixing to obtain mixture, and extracting with ethanol to obtain extract, i.e. Chinese medicinal powder.
The alcohol extraction method specifically comprises the steps of adding 95% by mass of ethanol solution into the mixture, leaching for 3 hours at normal temperature, filtering, taking the filtrate, and drying to obtain the traditional Chinese medicine powder, wherein the mass ratio of the mixture to the 95% by mass of ethanol solution is 1: 3.
< example 2>
The traditional Chinese medicine powder for helping melanin deposition of the black-bone chicken is mainly prepared from the following raw materials in parts by weight: 40 parts of eupolyphaga, 30 parts of elephant, 30 parts of medicinal grain thief, 30 parts of saw grain thief, 30 parts of earthworm, 20 parts of glossy privet fruit, 20 parts of crocus sativus, 15 parts of phoenix tree seed, 15 parts of ligusticum chuanxiong hort, 6 parts of salvia miltiorrhiza, 6 parts of poria cocos and 6 parts of seaweed.
Pulverizing Eupolyphaga Seu Steleophaga, Mixiang, medicinal Gusai, saw Gusai, Lumbricus, fructus Ligustri Lucidi, stigma croci Sativi, semen Firmianae, rhizoma Ligustici Chuanxiong, Saviae Miltiorrhizae radix, Poria, and Sargassum, mixing to obtain mixture, and extracting with ethanol to obtain extract, i.e. Chinese medicinal powder.
The alcohol extraction method specifically comprises the steps of adding 95% by mass of ethanol solution into the mixture, leaching for 3 hours at normal temperature, filtering, taking the filtrate, and drying to obtain the traditional Chinese medicine powder, wherein the mass ratio of the mixture to the 95% by mass of ethanol solution is 1: 3.
< example 3>
The traditional Chinese medicine powder for helping melanin deposition of the black-bone chicken is mainly prepared from the following raw materials in parts by weight: 38 parts of eupolyphaga, 28 parts of rice weevil, 28 parts of medicinal grain steatite, 27 parts of saw grain steatite, 27 parts of earthworm, 18 parts of glossy privet fruit, 17 parts of crocus sativus, 10 parts of phoenix tree seed, 10 parts of ligusticum chuanxiong hort, 4 parts of salvia miltiorrhiza, 5 parts of poria cocos and 4 parts of seaweed.
Pulverizing Eupolyphaga Seu Steleophaga, Mixiang, medicinal Gusai, saw Gusai, Lumbricus, fructus Ligustri Lucidi, stigma croci Sativi, semen Firmianae, rhizoma Ligustici Chuanxiong, Saviae Miltiorrhizae radix, Poria, and Sargassum, mixing to obtain mixture, and extracting with ethanol to obtain extract, i.e. Chinese medicinal powder.
The alcohol extraction method specifically comprises the steps of adding 95% by mass of ethanol solution into the mixture, leaching for 3 hours at normal temperature, filtering, taking the filtrate, and drying to obtain the traditional Chinese medicine powder, wherein the mass ratio of the mixture to the 95% by mass of ethanol solution is 1: 3.
< example 4>
The traditional Chinese medicine powder for helping melanin deposition of the black-bone chicken is mainly prepared from the following raw materials in parts by weight: 38 parts of eupolyphaga, 28 parts of rice weevil, 28 parts of medicinal grain steatite, 27 parts of saw grain steatite, 27 parts of earthworm, 18 parts of glossy privet fruit, 17 parts of crocus sativus, 10 parts of phoenix tree seed, 10 parts of ligusticum chuanxiong hort, 4 parts of salvia miltiorrhiza, 5 parts of poria cocos and 4 parts of seaweed.
Pulverizing Eupolyphaga Seu Steleophaga, Mixiang, medicinal Gusai, saw Gusai, Lumbricus, fructus Ligustri Lucidi, stigma croci Sativi, semen Firmianae, rhizoma Ligustici Chuanxiong, Saviae Miltiorrhizae radix, Poria, and Sargassum, mixing to obtain mixture, and extracting with ethanol to obtain extract, i.e. Chinese medicinal powder.
The alcohol extraction method specifically comprises the following steps: adding 95% by mass of ethanol solution into the mixture, leaching for 3h at normal temperature, performing suction filtration to obtain filtrate A and filter residue A, performing reduced pressure distillation on the filtrate A, volatilizing to remove ethanol to obtain ethanol extract, concentrating the ethanol extract to obtain clear paste with the relative density of 1.20, uniformly mixing the clear paste with silicon dioxide in a mass ratio of 1:3, and drying until the water content is lower than 5% to obtain the traditional Chinese medicine powder, wherein the mass ratio of the mixture to the 95% by mass of ethanol solution is 1: 3.
The method also comprises the step of performing freeze-thawing treatment on the mixture before the mixture is extracted by adopting an alcohol extraction method, and the specific method comprises the following steps:
stirring the mixture and drinking water at a mass ratio of 1:1 to obtain paste, freezing the paste at-25 deg.C for 25h, and standing at 25 deg.C until the paste is completely thawed.
The particle size of the mixture is less than 100 meshes.
Before the filtrate A is subjected to reduced pressure distillation, the method further comprises the following steps: adding 95% ethanol solution into the filter residue A according to the mass ratio of 1:3, leaching for 3h at normal temperature, filtering to obtain a filtrate B and a filter residue B, adding 95% ethanol solution into the filter residue B according to the mass ratio of 1:3, leaching for 3h at normal temperature, filtering to obtain a filtrate C, and adding the filtrate B and the filtrate C into the filtrate A.
< example 5>
The preparation method is the same as that of the embodiment 4, wherein the method further comprises the step of pretreating the mixture before the mixture is subjected to freeze-thaw treatment, and the specific method comprises the following steps:
adding a solution promoter into the mixture according to the mass ratio of 1:5, stirring uniformly, inserting a positive electrode and a negative electrode, switching on a current with the current intensity of 10mA, electrifying for 50min, and then carrying out ultrasonic treatment for 40min at the ultrasonic frequency of 80kHz and the temperature of 60 ℃, wherein the solution promoter specifically comprises the following components: uniformly mixing salt, sodium citrate, potassium dihydrogen phosphate, pineapple pulp juice and drinking water according to the mass ratio of 0.1:0.2:0.2:1: 2;
wherein, the pineapple pulp is pulped and filtered to obtain juice, and the pineapple pulp juice is obtained.
< comparative example 1>
The preparation method is the same as example 3, wherein the mixture is extracted by water extraction. The water extraction method specifically comprises the following steps: mixing the mixture with drinking water at a mass ratio of 1:10, leaching at 65 deg.C for 5 hr, filtering, and drying the filtrate to obtain Chinese medicinal powder.
< comparative example 2>
The preparation method is the same as example 3, wherein the mixture is extracted by microwave method. The microwave extraction method comprises the following steps: mixing the mixture with drinking water at a mass ratio of 1:10, placing in a microwave oven, performing microwave treatment on strong fire for 5min, filtering, and drying the filtrate to obtain Chinese medicinal powder.
< comparative example 3>
The preparation method is the same as that of example 3, wherein the formula of the traditional Chinese medicine powder is different, and the formula of the traditional Chinese medicine powder comprises 38 parts of eupolyphaga, 28 parts of rice weevil, 28 parts of medicinal grain steatile, 27 parts of saw grain steatile, 27 parts of earthworm, 18 parts of glossy privet fruit, 17 parts of crocus sativus and 10 parts of phoenix tree seed.
< comparative example 4>
The preparation was carried out as in example 4, wherein the mixture was not subjected to freeze-thaw treatment, nor was filtrate B and filtrate C added to filtrate A.
< comparative example 5>
The preparation was carried out as in example 4, wherein the mixture was not subjected to a freeze-thaw treatment.
< comparative example 6>
The preparation method is the same as that of example 5, wherein the pretreatment method is different and specifically comprises the following steps: adding the pineapple pulp juice into the mixture according to the mass ratio of 1:5, wherein the pineapple pulp is pulped and filtered to obtain the pineapple pulp juice.
< evaluation test of extraction method >
1. Detection of copper, tyrosine and vitamin B in the Chinese medicinal powder prepared by the method of example 3, comparative example 1 and comparative example 26The results of the alcohol extraction in example 3, the water extraction in comparative example 1 and the microwave extraction in comparative example 2 are shown in the following table:
TABLE 1
As shown in the above table, the extraction method of alcohol extracts copper, tyrosine, vitamin B in the Chinese medicinal powder6The content of the extract is obviously higher than the content of corresponding components in the traditional Chinese medicine powder prepared by a water extraction method and a microwave extraction method, which shows that the alcohol extraction method can improve the content of copper, tyrosine and vitamin B6The content of (a).
Detection of copper, tyrosine and vitamin B in the Chinese medicinal powder prepared by the method of example 4, comparative example 4 and comparative example 56The results are shown in the following table:
TABLE 2
| Group of | Copper% | Tyrosine% | Vitamin B6％ |
| Example 4 | 7.28 | 0.25 | 8.76 |
| Comparative example 4 | 4.24 | 0.23 | 5.89 |
| Comparative example 5 | 4.62 | 0.28 | 6.73 |
As can be seen from the above table, the Chinese medicinal powder prepared by the method of example 4 contains copper, tyrosine, vitamin B6The content of the active ingredients is obviously higher than the content of the corresponding ingredients in the traditional Chinese medicine powder prepared by the method of the comparative example 4 and the comparative example 5, which shows that the freeze-thaw treatment and the third alcohol extraction method can improve the content of copper, tyrosine and vitamin B6The content of (a).
2. The contents of phosphorus and polysaccharide in the powder of the Chinese medicine prepared by the methods of example 4, example 5 and comparative example 6 were measured, and the results are shown in the following table:
TABLE 3
| Group of | Phosphorus% | Polysaccharide% |
| Example 4 | 53.85 | 36.8 |
| Example 5 | 67.22 | 40.18 |
| Comparative example 6 | 54.29 | 36.12 |
As can be seen from the above table, the content of phosphorus and polysaccharide in the traditional Chinese medicine powder prepared by the method of example 5 is significantly increased compared to example 4;
compared with the comparative example 6, the effect of improving the content of phosphorus and polysaccharide in the traditional Chinese medicine powder is not obvious by only adding the pineapple pulp juice, not adding the accelerating liquid and simultaneously performing the current action, and the obvious effect can be obtained only by the accelerating liquid and combining the current action.
< influence of Chinese medicinal powder on the quality of Black-bone Chicken >
In 2018, 1 month and 9 days, 8000 Lingyun black-bone chickens of Lingyun black-bone chickens just coming out of shells are selected to be subjected to a feeding test, the chicken flocks are randomly divided into 8 groups, 1000 chickens are fed into each group, a control group is fed with basic feed, the traditional Chinese medicine powder prepared by the corresponding method of the basic feed plus 1000mg/kg is fed into the whole course of a brooding period in examples 1-5, the control group 3 and the control group 6, the brooding period is up to 150 days old, 20 Lingyun black-bone chickens are respectively selected from each group randomly, the melanin accumulation amount in livers, kidneys, chicken breasts and skins is detected, the fat and phospholipid content in chicken is detected, and the results are shown in tables 4 and 5.
The melanin content determination method comprises the following steps: accurately weighing 10mg of tissue sample, adding the tissue sample into 1mL of solution prepared from solulene-350: water according to the volume ratio of 9:1, heating in a water bath at 100 ℃ for dissolving, immediately placing the solution in a spectrophotometer to determine the absorbance value (A500) under the condition of 500nm, and comparing the melanin content of the tissue sample according to the absorbance value.
TABLE 4
| Group of | Liver disease | Kidney (A) | Chicken breast | Skin(s) |
| Control group | 0.108 | 0.102 | 0.089 | 0.147 |
| Example 1 | 0.135 | 0.123 | 0.115 | 0.170 |
| Example 2 | 0.136 | 0.128 | 0.120 | 0.178 |
| Example 3 | 0.139 | 0.125 | 0.118 | 0.179 |
| Example 4 | 0.147 | 0.135 | 0.130 | 0.189 |
| Example 5 | 0.154 | 0.141 | 0.134 | 0.195 |
| Comparative example 3 | 0.121 | 0.109 | 0.097 | 0.168 |
| Comparative example 6 | 0.142 | 0.131 | 0.120 | 0.182 |
As can be seen from the above table, compared with the control group, the traditional Chinese medicine powder prepared by the methods of examples 1 to 5, comparative example 3 and comparative example 6 is helpful for improving the melanin content of the liver, kidney, breast and skin of the black-bone chicken cock, which indicates that the traditional Chinese medicine powder prepared by the methods of examples 1 to 5 can assist the melanin deposition in the cock body, but the effect of comparative example 3 is significantly inferior to that of the traditional Chinese medicine powder prepared by the method of example 3.
TABLE 5
| Group of | Fat% | Phospholipid% |
| Example 3 | 3.58 | 5.42 |
| Example 6 | 3.06 | 5.68 |
| Comparative example 1 | 3.44 | 5.45 |
From the above table, after the traditional Chinese medicine powder of the example 5 is fed, the fat content in the chicken of the black-bone chicken can be reduced, the phospholipid content can also be increased, and the medicinal value of the black-bone chicken can be improved;
as can be seen from the comparison between example 4 and comparative example 6, the effect of the prepared traditional Chinese medicine powder on reducing the fat content in the chicken meat of the black-bone chicken is not obvious only by adding the pineapple pulp juice and not adding the promoting liquid and simultaneously performing the current action, but also the phospholipid content in the chicken meat of the black-bone chicken cannot be increased, and the traditional Chinese medicine powder prepared by only adding the promoting liquid and combining the current action can obtain the obvious effect on the two.
While embodiments of the invention have been described above, it is not limited to the applications set forth in the description and the embodiments, which are fully applicable to various fields of endeavor for which the invention may be embodied with additional modifications as would be readily apparent to those skilled in the art, and the invention is therefore not limited to the details given herein and to the embodiments shown and described without departing from the generic concept as defined by the claims and their equivalents.
Claims (5)
1. The preparation method of the traditional Chinese medicine powder helpful for melanin deposition of the black-bone chicken is characterized by being prepared from the following raw materials in parts by weight: 35-40 parts of ground beetle, 25-30 parts of rice weevil, 25-30 parts of medicinal grain steatite, 25-30 parts of saw grain steatite, 25-30 parts of earthworm, 15-20 parts of glossy privet fruit, 15-20 parts of crocus sativus, 7-15 parts of phoenix tree seed, 7-15 parts of ligusticum chuanxiong hort, 3-6 parts of salvia miltiorrhiza, 3-6 parts of poria cocos and 3-6 parts of seaweed;
pulverizing Eupolyphaga Seu Steleophaga, Mimosa, Cissus quadrangularis, Sambucus buergeriana, Sambucus serrata, Lumbricus, fructus Ligustri Lucidi, stigma croci Sativi, semen Firmianae, rhizoma Ligustici Chuanxiong, Saviae Miltiorrhizae radix, Poria, and Sargassum, mixing to obtain mixture, and extracting with alcohol to obtain extract as Chinese medicinal powder;
the method also comprises the step of performing freeze-thawing treatment on the mixture before the mixture is extracted by adopting an alcohol extraction method, and the specific method comprises the following steps:
stirring the mixture and drinking water according to the mass ratio of 1:1 to form paste, freezing the paste at the temperature of-30 to-20 ℃ for 20-30 h, and then placing the paste at the temperature of 20-30 ℃ until the paste is completely thawed;
the method also comprises the following steps of pretreating the mixture before performing freeze-thaw treatment on the mixture, wherein the method comprises the following specific steps:
adding a solution promoter into the mixture according to the mass ratio of 1:5, stirring uniformly, inserting a positive electrode and a negative electrode, switching on a current with the current intensity of 10mA, electrifying for 50min, and then carrying out ultrasonic treatment for 40min at the ultrasonic frequency of 80kHz and the temperature of 60 ℃, wherein the solution promoter specifically comprises the following components: uniformly mixing salt, sodium citrate, potassium dihydrogen phosphate, pineapple pulp juice and drinking water according to the mass ratio of 0.1:0.2:0.2:1: 2;
wherein, the pineapple pulp is pulped and filtered to obtain juice, and the pineapple pulp juice is obtained.
2. The method for preparing traditional Chinese medicine powder helpful for melanin deposition of black-bone chickens according to claim 1, wherein the alcohol extraction method specifically comprises: adding an ethanol solution with the mass fraction of 95% into the mixture, leaching for 2-4 hours at normal temperature, performing suction filtration to obtain a filtrate A and a filter residue A, performing reduced pressure distillation on the filtrate A, volatilizing to remove ethanol to obtain an ethanol extract, concentrating the ethanol extract to obtain a clear paste with the relative density of 1.15-1.25, uniformly mixing the clear paste with silicon dioxide in a mass ratio of 1:3, and drying until the water content is lower than 5% to obtain the traditional Chinese medicine powder, wherein the mass ratio of the mixture to the ethanol solution with the mass fraction of 95% is 1: 3.
3. The method of claim 1, wherein the particle size of the mixture is less than 100 mesh.
4. The method for preparing a powdered Chinese medicine for helping melanin deposition in a black-bone chicken as claimed in claim 1, further comprising, before distilling the filtrate A under reduced pressure: adding an ethanol solution with the mass fraction of 95% into the filter residue A according to the mass ratio of 1:3, leaching for 2-4 h at normal temperature, performing suction filtration to obtain a filtrate B and a filter residue B, adding an ethanol solution with the mass fraction of 95% into the filter residue B according to the mass ratio of 1:3, leaching for 2-4 h at normal temperature, performing suction filtration to obtain a filtrate C, and adding the filtrate B and the filtrate C into the filtrate A.
5. The use of the Chinese medicinal powder prepared by the preparation method according to any one of claims 2 to 4 for improving melanin deposition in Lingyun black-bone chicken cock.
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| CN102940146A (en) * | 2012-11-30 | 2013-02-27 | 江西省农业科学院畜牧兽医研究所 | Chinese herbal medicine feed additive for Taihe black-boned chickens |
| CN103519024A (en) * | 2013-10-27 | 2014-01-22 | 黑龙江省轻工科学研究院 | Method for manufacturing feed additive for feeding black-bone chicken |
| CN106071298A (en) * | 2016-06-07 | 2016-11-09 | 克拉玛依瑞恒畜牧开发有限责任公司 | A kind of feed for black bone chickens additive of pure Chinese medicine and its preparation method and application |
| CN106538875A (en) * | 2016-10-24 | 2017-03-29 | 牛保仁 | A kind of feed for black bone chickens additive containing humulus grass |
| CN107455598A (en) * | 2017-09-12 | 2017-12-12 | 葛洪伟 | Promote Chinese herbal feed additive of weanling pig growth and preparation method thereof |
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| CN109876062A (en) | 2019-06-14 |
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