CN109863143B - 提高基因组编辑效率的方法、组合物和试剂盒 - Google Patents
提高基因组编辑效率的方法、组合物和试剂盒 Download PDFInfo
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- CN109863143B CN109863143B CN201780052987.3A CN201780052987A CN109863143B CN 109863143 B CN109863143 B CN 109863143B CN 201780052987 A CN201780052987 A CN 201780052987A CN 109863143 B CN109863143 B CN 109863143B
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- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
- C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
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- C12N2310/00—Structure or type of the nucleic acid
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- C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
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Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201662361781P | 2016-07-13 | 2016-07-13 | |
| US201662361961P | 2016-07-13 | 2016-07-13 | |
| US62/361,961 | 2016-07-13 | ||
| US62/361,781 | 2016-07-13 | ||
| PCT/US2017/041979 WO2018013840A1 (en) | 2016-07-13 | 2017-07-13 | Methods, compositions and kits for increasing genome editing efficiency |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109863143A CN109863143A (zh) | 2019-06-07 |
| CN109863143B true CN109863143B (zh) | 2021-10-15 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201780052987.3A Active CN109863143B (zh) | 2016-07-13 | 2017-07-13 | 提高基因组编辑效率的方法、组合物和试剂盒 |
Country Status (9)
| Country | Link |
|---|---|
| US (2) | US11124805B2 (enExample) |
| EP (2) | EP4219462A1 (enExample) |
| JP (1) | JP7033583B2 (enExample) |
| CN (1) | CN109863143B (enExample) |
| AU (2) | AU2017295720B2 (enExample) |
| CA (1) | CA3030783A1 (enExample) |
| ES (1) | ES2938210T3 (enExample) |
| MA (1) | MA45670A (enExample) |
| WO (1) | WO2018013840A1 (enExample) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US12031150B2 (en) | 2016-07-13 | 2024-07-09 | Vertex Pharmaceuticals Incorporated | Methods, compositions and kits for increasing genome editing efficiency |
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| US9950282B2 (en) | 2012-03-15 | 2018-04-24 | Flodesign Sonics, Inc. | Electronic configuration and control for acoustic standing wave generation |
| US10704021B2 (en) | 2012-03-15 | 2020-07-07 | Flodesign Sonics, Inc. | Acoustic perfusion devices |
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| EP3215617B1 (en) | 2014-11-07 | 2024-05-08 | Editas Medicine, Inc. | Systems for improving crispr/cas-mediated genome-editing |
| US11021699B2 (en) | 2015-04-29 | 2021-06-01 | FioDesign Sonics, Inc. | Separation using angled acoustic waves |
| US11708572B2 (en) | 2015-04-29 | 2023-07-25 | Flodesign Sonics, Inc. | Acoustic cell separation techniques and processes |
| US11377651B2 (en) | 2016-10-19 | 2022-07-05 | Flodesign Sonics, Inc. | Cell therapy processes utilizing acoustophoresis |
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| US11124805B2 (en) | 2021-09-21 |
| MA45670A (fr) | 2019-05-22 |
| AU2017295720B2 (en) | 2021-07-22 |
| US20190225990A1 (en) | 2019-07-25 |
| JP7033583B2 (ja) | 2022-03-10 |
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| AU2021232813A1 (en) | 2021-10-14 |
| US12031150B2 (en) | 2024-07-09 |
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| US20220106615A1 (en) | 2022-04-07 |
| EP4219462A1 (en) | 2023-08-02 |
| CA3030783A1 (en) | 2018-01-18 |
| EP3484870A1 (en) | 2019-05-22 |
| ES2938210T3 (es) | 2023-04-05 |
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