A method of filamentous algae outburst in control giant clam seed large-scale production
Technical field:
The invention belongs to field of marine biotechnology, and in particular to filamentous algae in a kind of control giant clam seed large-scale production
The method of outburst.
Background technique:
Giant clam belongs to Mollusca lamellibranchiata curtain clam mesh Tridacnidae, is mainly distributed on the Indian Ocean, Western Pacific and China
The tropical forest insects reef sea area such as South Sea.In China, main giant clam species include Ku Shi giant clam, without squama giant clam, squama giant clam, Tridacna (chamestrachea) maxima (R ding),
The Che oyster that Nova giant clam, sarranine giant clam and Che oyster belong to.Giant clam is major architectural species in coral reef ecologic system, can occupy coral
60% or more coral reef Ecosystem Biomass, for maintaining coral reef bio-diversity, ecosystem stabilization to play positive key
Effect.Rise, under the background of seawater acidification in global ocean temperature, giant clam stock number is on a declining curve in the world, has
A little species have become extinct or endangered, and " Convention on International Trade in Endangered Species of Wild Fauna and Flora " is by the Ku Shi giant clam and squama in China
Giant clam is classified as protection species.Therefore, it is imperative to carry out giant clam resource recovery, is to solve by artificial breeding and large-scale farming
The most effective means of the problem.
During giant clam seed large-scale production, filamentous algae can largely be broken out in annual summer 7-8 month, seem net
In outstanding Zhang Yushui.Become flocculence when aging and fly at the water surface, be in brown color, feel is satiny.Filamentous algae is largely numerous in aquaculture pond
It grows, can seriously consume the inorganic salts in water body, it is suppressed that nutrient needed for zooxanthellae carries out photosynthesis in giant clam, into
And the growth of giant clam is adversely affected.When filamentous algae is in aquaculture pond mass propagation, as dragnet snarls giant clam, cause
It can not open outer embrane, carry out photosynthesis, ultimately cause giant clam massive mortality.It is Filamentous with water temperature, the raising of temperature
Algae is risen death in pond, and the toxic gases such as hydrogen sulfide are generated in decomposable process, improve the ammonia-nitrogen content in water, is reduced
Content of oxygen dissolved in water will cause giant clam poisoning, hypoxia death.It is previous using being chemically removed filamentous algae, although can be with
Filamentous algae is disposed, but also results in the mortality of giant clam seed.It can be seen that developing a kind of not damaged prevention and control filamentous algae
Method to giant clam artificial breeding and large-scale production have important theoretical value and application prospect.
Summary of the invention:
In order to solve the problems, such as that current filamentous algae outburst leads to giant clam seed mortality, the present invention provides a kind of controls
The method that filamentous algae is broken out in giant clam seed large-scale production provides reliable technology for giant clam artificial breeding and large-scale production
Guarantee.
The method of filamentous algae outburst in control giant clam seed large-scale production of the invention, comprising the following steps:
Physical method: a in the season of filamentous algae outburst, will be attached in the attachment base of giant clam juvenile mollusk with banister brush first
Filamentous algae brush, then proceed to be blown off remaining filamentous algae with suction pipe, next will cultivate cylinder bottom and casing wall with scrub-brush
Filamentous algae dispose, change water after completing;
Biological method: b after the processing of step a physical method, puts top shell, red mouth spiral shell and tropical fish, horse in a suitable place to breed in culturing jar
The breeding density of hoof spiral shell is 8-10/m2, the breeding density of red mouth spiral shell is 4-6/m2, the breeding density of tropical fish is 1 tail/m2;
It is cleaned up in time the case where giant clam shellfish if any dead individual in observation culturing jar daily.
Filamentous algae can be quickly removed by physical method, simple and convenient, effect is good;But this method workload is very big,
Filamentous algae is easy secondary outburst, is only suitable for the filamentous algae adhered in the solid objects such as corallite removing;
It can control the quantity of algae with algae biology is removed, currently, a kind of biology is " Almightiness type ", most of lifes not yet
Object all only has certain anti-removing algae effect in one aspect.Horse base of a fruit spiral shell and red mouth spiral shell mainly remove corallite and giant clam shell table
The various filamentous algaes of attachment, raising are easier to;Big sail, which falls the tropical fish such as porgy, can mainly remove the filamentous algae of casing wall and cylinder bottom.It should
Method safety, laborsaving, filamentous algae elimination effect is splendid, will not repeatedly, and the duration is long.But when giant clam seedling is also lesser
It waits, this method is easy to cause giant clam seedling injured or even death, therefore be suitable for the giant clam seedling filamentous algae of larger individual (> 1cm)
Prevention and control.
The tropical fish is preferably that big sail falls porgy.
The giant clam is preferably without squama giant clam Tridacna derasa or squama giant clam Tridacna squamosa.
The attachment base is preferably corallite.
During the large-scale production of giant clam seedling, in the season of filamentous algae outburst, first by physical method, fur is used
After brush, suction pipe and scrub-brush dispose obstinate filamentous algae, porgy is then fallen by top shell, red mouth spiral shell, big sail and continues to remove
Remaining algae.Biology and physical method, which combine, in this way can effectively control filamentous algae in giant clam seedling large-scale production process
Outburst.
The beneficial effects of the present invention are: method of the invention has easy to operate, feasibility height, and it is practical, it is easy to push away
The advantages that wide.Method of the invention substantially increases the survival rate of giant clam seedling Transitional culture, significantly improves giant clam scale metaplasia
The efficiency of production improves the survival rate of giant clam to 95% or more, and the large-scale production for giant clam seed provides practical basis, is
The resource recovery of giant clam provides technical guarantee.
Detailed description of the invention:
Fig. 1 is that physical method removes filamentous algae;Wherein, A-B, suction pipe blow algae;C, the clear algae of banister brush;D, adhere to Che before processing
The corallite of giant clam seedling;Adhere to the corallite of giant clam seedling after E-F, processing.
Fig. 2 is biological method control filamentous algae;Wherein, a-f is top shell, red mouth spiral shell and the big sail control of falling porgy filamentous algae.
Specific embodiment:
The following examples are further illustrations of the invention, rather than limiting the invention.
Embodiment 1
A, physical method: in May, 2017 is at Chinese Academy of Science Nanhai Ocean Research Institute, the torrid zone, Sanya marine biological laboratory station
Giant clam breeding is carried out, more than 80 ten thousand squama giant clam juvenile mollusk is obtained.Since illumination is strong when July summer, temperature is high, Filamentous
Algae large-scale outbreak overgrows with filamentous algae on squama giant clam juvenile mollusk, culturing jar bottom, casing wall and attachment base corallite, we use first
Banister brush gently brushes the filamentous algae on the corallite for being attached to squama giant clam juvenile mollusk, then proceedes to remaining filiform with suction pipe
Algae is blown off, next removes the filamentous algae for cultivating cylinder bottom and casing wall and other algae together with scrub-brush, changes water after completing,
The large-scale outbreak of filamentous algae can be prevented by these methods;
B, biological method: after disposing most filamentous algae by physical method, due to filiform remaining on corallite
Filamentous algae spore can still result in the secondary outburst of filamentous algae in algae and seawater, therefore we continue to control silk using algae biology is removed
Shape algae.It is put into 50 top shells in the culturing jar of 5 squared magnitudes, 30 red mouth spiral shells and the big sail of 5 tails fall porgy, top shell and red
Mouthful spiral shell mainly removes the various filamentous algaes adhered to corallite and giant clam, and big sail, which falls porgy, can mainly remove casing wall and cylinder bottom
Filamentous algae.Situation in observation culturing jar daily, cleans up in time if any dead individual.After two weeks, on corallite with
And the filamentous algae with giant clam juvenile mollusk significantly reduces, filamentous algae is completely removed after one month, and squama giant clam seedling state growth is good,
Survival rate is 95% or more.
Relative to conventional method, giant clam seed survival rate after filamentous algae outburst usually≤30% or even entire PLA's destruction, and
Design method provided by the invention obtains higher survival efficiency, survival rate >=95%.
Embodiment 2
A, physical method: in May, 2018 is at Chinese Academy of Science Nanhai Ocean Research Institute, the torrid zone, Sanya marine biological laboratory station
Carry out giant clam breeding, obtain more than 30 ten thousand without squama giant clam juvenile mollusk.Since illumination is strong when month summer 7-8, temperature is high,
Filamentous algae large-scale outbreak overgrows with filamentous algae on no squama giant clam juvenile mollusk, culturing jar bottom, casing wall and attachment base corallite, we
The filamentous algae on the corallite for being attached to no squama giant clam juvenile mollusk is gently brushed with banister brush first, then proceeding to will be residual with suction pipe
Remaining filamentous algae is blown off, is next removed the filamentous algae for cultivating cylinder bottom and casing wall and other algae together with scrub-brush, is completed
Water is changed later, and the large-scale outbreak of filamentous algae can be prevented by these methods.
B, biological method: after disposing most filamentous algae by physical method, due to filiform remaining on corallite
Filamentous algae spore can still result in the secondary outburst of filamentous algae in algae and seawater, therefore we continue to control silk using algae biology is removed
Shape algae.Be put into 80 top shells in the culturing jar of 10 squared magnitudes, 40 red mouth spiral shells and the big sail of 10 tails fall porgy, top shell and
Red mouth spiral shell mainly removes the various filamentous algaes adhered to corallite and giant clam, and big sail, which falls porgy, can mainly remove casing wall and cylinder bottom
Filamentous algae.Situation in observation culturing jar daily, cleans up in time if any dead individual.After two weeks, on corallite
And significantly reduced without the filamentous algae with squama giant clam juvenile mollusk, filamentous algae is completely removed after one month, no squama giant clam seedling state
Well-grown, survival rate are 95% or more.
Relative to conventional method, giant clam seed survival rate after filamentous algae outburst usually≤30% or even entire PLA's destruction, and
Design method provided by the invention obtains higher survival efficiency, survival rate >=95%.