CN109717346A - A kind of method of the fresh-keeping cordyceps sinensis of irradiation method - Google Patents
A kind of method of the fresh-keeping cordyceps sinensis of irradiation method Download PDFInfo
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Abstract
The present invention provides a kind of method of fresh-keeping cordyceps sinensis of irradiation method, is related to cordyceps sinensis technical field of preservation of fresh, first impregnates fresh cordyceps sinensis in fresh-keeping liquid, then carry out controlled atmospheric packing, irradiates to the cordyceps sinensis of controlled atmospheric packing, with60Co gamma-rays or 5~10MeV electron beam irradiate the cordyceps sinensis of controlled atmospheric packing, and irradiation dose is 0.1~1.5kGy, unevenness≤1.25, to realize to the fresh-keeping of cordyceps sinensis.As shown in the embodiment of present invention, Preservation Treatment is carried out to fresh cordyceps sinensis using the method for the invention, weight-loss ratio, soluble protein content rate of descent and the polyphenol oxidase activity growth rate of cordyceps sinensis have significant decrease compared with the existing technology, and fresh keeping time at least can achieve 75d.The complex of animal-fungal tissue this kind of for cordyceps sinensis can be realized long-term, effective fresh-keeping under the irradiation dose that the present invention limits, and will not cause significantly to injure to cordyceps sinensis.
Description
Technical field
The present invention relates to cordyceps sinensis technical field of preservation of fresh more particularly to a kind of methods of the fresh-keeping cordyceps sinensis of irradiation method.
Background technique
Cordyceps sinensis is a kind of high-grade nourishing rare traditional Chinese medicine, nutriment and active constituent containing there are many, including nucleosides,
Polysaccharide, sterol, fatty acid, protein, polypeptide and volatile component etc. have antitumor, adjusting immune system, promotion body thin
Cellular damage reparation, free radical resisting, anti-oxidant, anti-aging, protection cardiac muscle and vascular cell improve cardiovascular function, protection liver
Dirty, protection kidney adjusts respiratory system, adjusts hematological system and adjusts the functions such as blood lipid.
Cordyceps sinensis on the market is dry cordyceps sinensis, a large amount of volatile components in cordyceps sinensis drying process mostly at present
It can evaporate, part nutrition can be lost, and active constituent can inactivate, while being taken mouthfeel and being greatly reduced, and fresh and alive cordyceps sinensis can be most
Its nutriment of the reservation of limits and active constituent, and keep its unique faint scent and crispy texture.In addition, pharmacology
Studies have shown that fresh cordyceps sinensis is in the pharmacological actions such as immunological regulation, antitumor, anti-oxidant compared with dry cordyceps sinensis
Aspect all has better effects.Therefore, the fresh-keeping of fresh cordyceps sinensis is urgently to be solved in the process of manufacture faced at present
Most significant problems.
Preservation method about fresh cordyceps sinensis is one of the hot spot direction of cordyceps sinensis research in recent years, studies main collection
In preservation by low temperature, quick-freezing fresh-keeping, in terms of, related patents method is as follows:
((CN 102406164A) discloses a kind of fresh and alive cordyceps sinensis processing and its can preparation side to Chinese patent application
Method fresh cordyceps sinensis will be mainly classified after cleaning in -70 DEG C of stored frozens of ultralow temperature, rear thawed by cordyceps sinensis,
It is immersed in the tap water fresh-keeping liquid containing citric acid, sodium isoascorbate, is examined, high temperature sterilization is prepared into can.
Chinese invention patent (CN 1037405928) discloses a kind of preservation method of cordyceps sinensis, to cordyceps sinensis
Head, after stroma and the cleaning of polypide branch, nutriment and active material are lost in order to prevent, by the fresh winter worm summer after cleaning
Grass, which is placed in 0.9% acid calcium lactate solution, impregnates 5-8min: quickly cooling handles 5~12h of cordyceps sinensis under the conditions of -75 DEG C again
Afterwards, it is placed in -5~-25 DEG C of vacuum cold case and saves.
Chinese invention patent (CN 1021782268) discloses a kind of fresh processing technique of cordyceps sinensis, mainly will
Fresh cordyceps sinensis deepfreeze under conditions of being lower than -65 DEG C；Mode cleaning and sterilization of the ultrasonic wave in conjunction with ozone is set simultaneously,
It is saved at last 0 DEG C.
Chinese patent application (105199956 A of CN) discloses a kind of preservation method of cordyceps sinensis, mainly uses one
After determining the cleaning of concentration alcoholic solution, being sprayed on cordyceps sinensis surface with hydromel saves nutrient water from damage, then carries out air-drying dehumidifying
Processing, then quick-frozen at 30 DEG C be placed on -5 DEG C of cold storage warehouses below and stored.
Chinese invention patent (106727734 A of CN) discloses a kind of fresh cordyceps sinensis product and preparation method thereof, mainly
It is by fresh cordyceps sinensis using flowing water and after being cleaned by ultrasonic, atomizing humidifying performance handles 10-40min, then uses 3-7%O2,75-
92%N2And 5-18%CO2Controlled atmospheric packing is carried out with fresh cordyceps sinensis is compared, the cordycepic acid content of fresh cordyceps sinensis product is not low
In 2%, polysaccharide is not less than 0.5%.
Chinese invention patent (106720232 A of CN) discloses a kind of storing and fresh-keeping method of fresh cordyceps sinensis, mainly
The methods of saved using Low-temperature cleaning, low temperature classification, classification, entire technique carries out at low ambient temperatures, makes fresh cordyceps sinensis
Fresh keeping time greatly prolongs, and reduces the loss of effective component, while reducing fresh-keeping cost.
Some fresh-keeping thinkings of the above prior art are saved in such a way that direct superfreeze is situated between 70 DEG C, Yi Xieshi
(0 DEG C or less) of low temperature preservation after the quick-frozen latching effective component of ultralow temperature (- 30 DEG C to -75 DEG C), also some technologies are sought to save from damage
It supports moisture or keeps color, increase the step of impregnating sour water or sprinkling hydromel, these prior arts provide fresh winter worm
Summer grass, compares dry cordyceps sinensis product and remains most nutriment and active constituent, but above-mentioned preservation method is big
Mostly using sense organ method from appearance color, smell, hardness, mouthfeel, flavour etc. variation aspect judgement sample freshness, it is subjective because
Element interference greatly, not can effectively solve loss of water in preservation process, soluble protein content decline, polyphenol oxidase enzyme activity still
Property increase etc. caused by cordyceps sinensis freshness decline the problem of.
The water content of the cordyceps sinensis of fresh acquisition is higher, and in storage period, its moisture content factor solid object surface steams
It rises and acts on and scatter and disappear, weightlessness can cause the symptoms such as sample softening physiology that its freshness is caused to decline.Soluble protein participates in
The regulation of a variety of Physiological and Biochemical Metabolism processes of edible mushroom is one of the important indicator of edible mushroom quality and nutrition.Under its content
Drop is considered as adopting an important feature of rear sample aging, with the extension of storage time, due to lacking in nitrogen source and mushroom body
The enhancing of prolease activity, accelerates the decomposition of protein, to change the flavor of mushroom body.Polyphenol oxidase is edible
The main enzyme of bacterium enzymatic browning can be such that product brown stain even ruins, the serious nutritive value for damaging edible mushroom, flavor and outer
Texture is seen, polyphenol oxidase activity is lower to illustrate that storage property can be better.
Summary of the invention
The present invention mentions to overcome existing fresh cordyceps sinensis preserving type to device dependence height, cumbersome defect
Supplied it is a kind of be suitable for fresh-keeping fresh cordyceps sinensis method, easy to operate, for a long time fresh-keeping fresh winter worm summer small to device dependence
Grass product matter is good.
In order to achieve the above-mentioned object of the invention, the present invention the following technical schemes are provided:
The present invention provides a kind of methods of the fresh-keeping cordyceps sinensis of irradiation method, comprising the following steps:
(1) fresh cordyceps sinensis is soaked in fresh-keeping liquid, takes out drying, the cordyceps sinensis after being impregnated；
The fresh-keeping liquid includes 1~3mmol/L D-sorbite, 1~3mmol/L vitamin C or ISOASCORBIC ACID, 6~9 μ
Mol/L phytic acid, the L-cysteine of 0.4~0.8mg/kg, the ethanol extract of 30~100mg/ml Cordyceps fermented liquid, 50
~200mg/L chitosan oligosaccharide and 100~200mg/L antibacterial peptide；
(2) cordyceps sinensis after immersion is packed into packaging bag, be filled with the mixed gas that is made of oxygen and carbon dioxide into
Row controlled atmospheric packing obtains the cordyceps sinensis of controlled atmospheric packing；
(3) with60Co gamma-rays or 5~10MeV electron beam irradiate the cordyceps sinensis of controlled atmospheric packing, by the winter worm after irradiation
The refrigeration of summer grass.
Preferably, in step (1), the fresh cordyceps sinensis is soaked in fresh-keeping liquid again by ultrasonic cleaning.
Preferably, the power of the ultrasonic cleaning is 250~1200W, and the frequency of ultrasonic cleaning is 40kHz~120kHz,
The number of ultrasonic cleaning is 2~5 times, and the time being cleaned by ultrasonic every time independently is 5~10min.
Preferably, in step (1), the preparation method of the ethanol extract of the Cordyceps fermented liquid includes following step
It is rapid:
S1, cordyceps sinensis is inoculated in liquid fermentation medium, cultivates 6~8d, be separated by solid-liquid separation, obtain cordyceps sinensis hair
Zymotic fluid；
S2, the concentration expressed in percentage by volume that ethyl alcohol to ethyl alcohol are added into Cordyceps fermented liquid reach 70~80%, are centrifuged
Supernatant removes drying after ethyl alcohol, obtains the ethanol extract of Cordyceps fermented liquid.
Preferably, the liquid fermentation liquid in the step S1 includes potato, soya bean, sucrose, glucose, peptone, ferment
One of female cream, mineral salt and vitamin are a variety of.
Preferably, in step (1), the antibacterial peptide includes streptococcus lactis antibacterial peptide, streptomyces antibacterial peptide and piece coccus
One of antibacterial peptide is a variety of.
Preferably, the drying temperature in the step (1) is -2~10 DEG C.
Preferably, in the step (2), the volume ratio of oxygen and carbon dioxide in mixed gas be 55~65:35~
45。
Preferably, the refrigerated storage temperature in the step (3) is 0~4 DEG C.
Compared with prior art, beneficial effects of the present invention:
The present invention provides a kind of methods of the fresh-keeping cordyceps sinensis of irradiation method, first soak fresh cordyceps sinensis in fresh-keeping liquid
Bubble, then controlled atmospheric packing is carried out, the cordyceps sinensis of controlled atmospheric packing is irradiated, is realized to the fresh-keeping of cordyceps sinensis.Conventional spoke
Irradiated targets usually are carried out to food across packaging film to after food packaging according to fresh-keeping, studies have shown that, after radiation treatment
Pack rupture of membranes, food rot, brown stain and in terms of all significantly lower than without through irradiation control group.If
Cryopreservation is carried out simply after radiation sterilizing, the fresh keeping time of cordyceps sinensis is unable to satisfy only in 30d or so to fresh winter worm
The requirement of summer grass progress long-period freshness preserving.As shown in the embodiment of present invention, using the method for the invention to fresh cordyceps sinensis into
Row Preservation Treatment, the weight-loss ratio of cordyceps sinensis, soluble protein content rate of descent and polyphenol oxidase activity growth rate are opposite
There is significant decrease in the prior art, fresh keeping time at least can achieve 75d.
Meanwhile the irradiation of high dose can damage material itself, and caused by the raising with irradiation dose
Injury is more serious, thus the determination of irradiation dose is the key element of preservation method of the present invention.The irradiation that the present invention limits
The complex of animal-fungal tissue this kind of for cordyceps sinensis can be realized long-term, effective fresh-keeping under dosage, and will not be to the winter
Worm summer grass causes significantly to injure.
Specific embodiment
The present invention provides a kind of methods of the fresh-keeping cordyceps sinensis of irradiation method, comprising the following steps:
(1) fresh cordyceps sinensis is soaked in fresh-keeping liquid, takes out drying, the cordyceps sinensis after being impregnated；
The fresh-keeping liquid includes 1~3mmol/L D-sorbite, 1~3mmol/L vitamin C or ISOASCORBIC ACID, 6~9 μ
Mol/L phytic acid, the L-cysteine of 0.4~0.8mg/kg, the ethanol extract of 30~100mg/ml Cordyceps fermented liquid, 50
~200mg/L chitosan oligosaccharide and 100~200mg/L antibacterial peptide；
(2) cordyceps sinensis after immersion is packed into packaging bag, be filled with the mixed gas that is made of oxygen and carbon dioxide into
Row controlled atmospheric packing obtains the cordyceps sinensis of controlled atmospheric packing；
(3) with60Co gamma-rays or 5~10MeV electron beam irradiate the cordyceps sinensis of controlled atmospheric packing, irradiation dose 0.1
~1.5kGy, unevenness≤1.25 refrigerate the cordyceps sinensis after irradiation.
Fresh cordyceps sinensis is soaked in fresh-keeping liquid by the present invention, takes out drying, the cordyceps sinensis after being impregnated.The present invention
The fresh-keeping liquid of use includes composite antioxidant and compound preservative, the limitation for avoiding the fresh-keeping approach of single antistaling agent single
Property, preservation is played in collaboration.
Fresh cordyceps sinensis of the present invention refers to that acquisition time is no more than the cordyceps sinensis not being dried for 24 hours and.This hair
The bright preservation method is to protect the quality of fresh cordyceps sinensis not significantly change.
Fresh cordyceps sinensis of the present invention is preferably cleaned before being soaked in fresh-keeping liquid, and the cleaning agent of the cleaning is excellent
Choosing includes water or calcium lactate solution.The purpose that the present invention cleans fresh cordyceps sinensis be in order to remove the impurity such as silt, not into
Row cleaning can also realize the fresh-keeping effect of preservation method of the present invention.In the present invention, the quality hundred of the calcium lactate solution
Point concentration is preferably 0.05~1%, and more preferably 0.1~0.5%.
In the present invention, the method for the cleaning is preferably cleaned by ultrasonic, using ultrasonic wave cavitation in a liquid,
Acceleration effect and direct flow effect to liquid and dirt directly, indirectly effect, make crud layer by dispersion, emulsification, removing and
Reach and rapidly and efficiently clean fresh cordyceps sinensis surface risk pollutant purpose, Conventional cleaning methods is avoided to damage in the process of cleaning
Sample surfaces.Currently preferred, the power of the ultrasonic cleaning is 250~1200W；More preferably 600~900W.The present invention
Preferably, the frequency of the ultrasonic cleaning is preferably 40kHz~120kHz；More preferably 70~80kHz.It is currently preferred,
The number of the ultrasonic cleaning is preferably 2~5 times, and more preferably 3 times；The present invention is it is further preferred that be every time cleaned by ultrasonic
Time independently is 5~10min, is still more preferably 6~8min.
In the present invention, the fresh-keeping liquid includes that 1~3mmol/L D-sorbite, 1~3mmol/L vitamin C or different dimension are raw
Plain C, 6~9 μm of ol/L phytic acid, the L-cysteine of 0.4~0.8mg/kg, 30~100mg/ml Cordyceps fermented liquid ethyl alcohol
Extract, 50~200mg/L chitosan oligosaccharide and 100~200mg/L antibacterial peptide；It preferably include 1.5~2mmol/L D-sorbite, 2
~2.5mmol/L vitamin C or ISOASCORBIC ACID, 7~8 μm of ol/L phytic acid, the L-cysteine of 0.5~0.6mg/kg, 50~
Ethanol extract, 100~150mg/L chitosan oligosaccharide and the 120~160mg/L antibacterial peptide of 80mg/ml Cordyceps fermented liquid.
In the present invention, D-sorbite has the function of moisture absorption, water conservation, the D-sorbite in fresh-keeping liquid of the present invention
It is used as water-retaining agent.
In the present invention, the vitamin C or ISOASCORBIC ACID use in fresh-keeping liquid as antioxidant, can keep fresh
The color and natural flavor of cordyceps sinensis, prolong storage period, and without any side effects.
In the present invention, phytic acid uses in fresh-keeping liquid as antioxidant, metal ion chelation agent and antistaling agent.Phytic acid
Hydrolysate is inositol and phosphatide in human body, and inositol has anti-aging effect, and phosphatide is the important component of human body cell.
Meanwhile each matter acid molecule can provide six pairs of hydrogen atoms, make free radical electronically forms rock-steady structure, thus instead of by fresh-keeping
Cordyceps sinensis is used as oxygen molecule, avoids fresh cordyceps sinensis oxidation deterioration.
In the present invention, L-cysteine uses in fresh-keeping liquid as BPH resistant rice variety antistaling agent, to prevent vitamin C or different
Vitamin C oxidation, can also prevent the brown stain of cordyceps sinensis surface.
In the present invention, the principle active component in the ethanol extract of the Cordyceps fermented liquid is guanine, worm
Careless element, hypoxanthine, cytidine, xanthine and adenosine, rise in fresh-keeping liquid of the present invention and use as antimicrobial antistaling agent, inhibit
Bacterial reproduction in preservation process.
Specifically, the ethanol extract of Cordyceps fermented liquid of the present invention is preferably according to the side included the following steps
It is prepared by method:
S1, cordyceps sinensis is inoculated in liquid fermentation medium, cultivates 6~8d, be separated by solid-liquid separation, obtain cordyceps sinensis hair
Zymotic fluid；
S2, the concentration expressed in percentage by volume that ethyl alcohol to ethyl alcohol are added into Cordyceps fermented liquid reach 70~80%, are centrifuged
Supernatant removes drying after ethyl alcohol, obtains the ethanol extract of Cordyceps fermented liquid.
In the present invention, the liquid fermentation medium preferably includes but is not limited to potato, soya bean, sucrose, grape
One of sugar, peptone, yeast extract, mineral salt and vitamin are a variety of.In the present invention, the cordyceps sinensis is inoculated with liquid
The inoculum concentration of fermentation medium is preferably 0.01~0.1g/ml, more preferably 0.05g/ml.In the present invention, the culture
Time is preferably 6~8d, more preferably 7d.In the present invention, the temperature of the culture is preferably 22~30 DEG C, more preferably 26
℃.The present invention is not particularly limited the solid-liquid separating method, using manner known in the art, such as filtering or centrifugation
Deng.
It is big in order to precipitate removal that it is 70~80% that the present invention, which adds ethyl alcohol to concentration of alcohol into Cordyceps fermented liquid,
The impurity such as molecular polysaccharide, albumen, and active principle is enriched in supernatant.In the present invention, the revolving speed of the centrifugation is preferred
For 5000~15000rpm, more preferably 10000rpm.In the present invention, the time of the centrifugation is preferably 15~60min, more
Preferably 30min.The present invention is not particularly limited to how to remove ethyl alcohol, is steamed using method commonly used in the art, such as rotation
Hair.The present invention is not particularly limited the mode of the drying, using methods known in the art, such as heated-air drying, subtracts
It press dry dry.
In the present invention, chitosan uses in fresh-keeping liquid as antisepsis antistaling agent.Chitosan is a kind of natural food
Preservative has stronger antibacterial and antioxidation, additionally it is possible to effectively inhibit the respiratory intensity in winter worm summer to increase, delay solvable
Property the nutriments such as solid content and titratable acid be lost, reduce weight-loss ratio, prevent from softening.
In the present invention, antibacterial peptide uses in fresh-keeping liquid as bacteriostasis, preservation agent, for prolonging storage period, substitutes antibiosis
Element.In the present invention, in the preferred streptococcus lactis antibacterial peptide of the antibacterial peptide, streptomyces antibacterial peptide and piece coccus antibacterial peptide
It is one or more.
In the present invention, it is preferably 2~5min that the fresh cordyceps sinensis, which is soaked in the time in fresh-keeping liquid, and more preferably 3
~4min.The present invention is not particularly limited the dosage of fresh-keeping liquid, can submerge to fresh-keeping cordyceps sinensis.
In the present invention, the temperature of the cordyceps sinensis drying after the fresh-keeping liquid by immersion is preferably -2~10 DEG C, more excellent
It is selected as 0~4 DEG C.The present invention is in order to avoid because high temperature causes the corruption of cordyceps sinensis to accelerate in using the purpose of low temperature drying
Portion's moisture loss and the loss of nutritional ingredient, heat-sensitive substance.
After cordyceps sinensis after being impregnated, the cordyceps sinensis after immersion is packed into packaging bag by the present invention, is filled with by oxygen
Controlled atmospheric packing is carried out with the mixed gas of carbon dioxide composition, obtains the cordyceps sinensis of controlled atmospheric packing.The present invention uses controlled atmosphere packet
The mode of dress extends the freshness date of cordyceps sinensis.
In the present invention, the volume ratio of oxygen and carbon dioxide is preferably 55~65:35~45 in the mixed gas, more
Preferably 60:40.The present invention is not particularly limited the amount for being filled with mixed gas.
After obtaining the cordyceps sinensis of controlled atmospheric packing, the present invention with60Co gamma-rays or 5~10MeV electron beam are to controlled atmospheric packing
Cordyceps sinensis irradiation, irradiation dose be 0.1~1.5kGy, unevenness≤1.25, by after irradiation cordyceps sinensis refrigerate.This
The irradiation dose that invention limits is fresh-keeping suitable for this animal of cordyceps sinensis-plant complex, i.e., will not make to cordyceps sinensis
It is fresh-keeping in the long period at also can reach compared with havoc.As shown in the embodiment of the invention, it is handled according to the method for the invention
The fresh-keeping 75d of cordyceps sinensis will not significantly change its color, smell and taste afterwards, and treated that cordyceps sinensis can be real for preservation method of the present invention
Now the longer time is fresh-keeping.
The method of the fresh-keeping cordyceps sinensis of irradiation method provided by the invention combines composite preservative, controlled atmospheric packing and irradiation and protects
Fresh processing can effectively extend the fresh-keeping of cordyceps sinensis, delay the weight-loss ratio of cordyceps sinensis fresh goods to increase, the drop of soluble protein
Low and polyphenol oxidase activity growth.
Technical solution provided by the invention is described in detail below with reference to embodiment, but they cannot be understood
For limiting the scope of the present invention.
Embodiment 1
At 0 DEG C, with head, stroma and the polypide of the linear flowing water cordyceps sinensis with soil collected to the place of production according to
It is secondary to be cleaned；It is ultrasonic cleaning in 0.1% calcium lactate solution that fresh cordyceps sinensis after cleaning, which is placed in mass percentage concentration,
5 times (power 250W, frequency 120Hz, between 0 DEG C of temperature, the time 5/ time), 0 DEG C drains surface moisture.
Prepare fresh-keeping liquid: 3mmol/L D-sorbite, 3mmol/LVc, 6 μm of ol/L phytic acid, L-cysteine concentration are
The ethanol extract of 0.4mg/kg, 30mg/mL Cordyceps fermented liquid, 50mg/L chitosan oligosaccharide, 100mg/L streptococcus lactis antibacterial
Peptide (Nisin, manufacturer: Shandong Fu Ruida Biotechnology Co., Ltd).
The preparation of the ethanol extract of Cordyceps fermented liquid: liquid fermentation training is inoculated in after aweto strain is activated
It supports in base (including potato, soya bean, sucrose, glucose, peptone, yeast extract, mineral salt and vitamin), is cultivated at 26 DEG C
7d, centrifuging and taking supernatant, obtains Cordyceps fermented liquid；The volume basis of ethyl alcohol to ethyl alcohol is added into Cordyceps fermented liquid
Concentration stops when being 75%, and supernatant, the ethyl alcohol in vacuum distillation removal supernatant is collected by centrifugation, and freeze-drying obtains winter worm
The ethanol extract of summer grass fermentation liquid.
Fresh cordyceps sinensis after ultrasonic cleaning is impregnated into 2min, 0 DEG C of cold wind drying, the winter after being impregnated in fresh-keeping liquid
Worm summer grass.Cordyceps sinensis after immersion is fitted into packaging bag, it will be by oxygen and carbon dioxide volume ratio 55 with gas-control packing device:
45 mixed gas is filled with packaging bag, obtains the cordyceps sinensis of controlled atmospheric packing.
With60Co gamma-rays irradiates the cordyceps sinensis of controlled atmospheric packing, irradiation dose 0.1kGy, unevenness≤1.25.0
DEG C refrigeration.
Embodiment 2
At 10 DEG C, with head, stroma and the polypide of the linear flowing water cordyceps sinensis with soil collected to the place of production
Successively cleaned；It is in 0.5% calcium lactate solution that fresh cordyceps sinensis after cleaning, which is placed in mass percentage concentration, and ultrasonic wave is clear
It washes 1 time (power 1200W, frequency 40kHz, between 10 DEG C of temperature, time 5min/ times), 10 DEG C drain surface moisture.
Prepare fresh-keeping liquid: the different Vc of 1mmol/L D-sorbite, 1mmol/L, 9 μm of ol/L phytic acid, L-cysteine concentration are
Ethanol extract, 200mg/L chitosan oligosaccharide, the 200mg/L streptomyces antibacterial peptide of 0.8mg/kg, 100mg/mL Cordyceps fermented liquid
(epsilon-polylysine, ε-Polylysine, manufacturer: Zhengzhou Bai Nafo bioengineering limited liability company)；
The preparation of the ethanol extract of Cordyceps fermented liquid: liquid fermentation training is inoculated in after aweto strain is activated
It supports in base (including potato, soya bean, sucrose, glucose, peptone, yeast extract, mineral salt and vitamin), is cultivated at 24 DEG C
7d, centrifuging and taking supernatant, obtains Cordyceps fermented liquid；The volume basis of ethyl alcohol to ethyl alcohol is added into Cordyceps fermented liquid
Concentration stops when being 70%, and supernatant, the ethyl alcohol in vacuum distillation removal supernatant is collected by centrifugation, and freeze-drying obtains winter worm
The ethanol extract of summer grass fermentation liquid.
Fresh cordyceps sinensis after ultrasonic cleaning is impregnated into 5min, 10 DEG C of cold wind dryings, after being impregnated in fresh-keeping liquid
Cordyceps sinensis.Cordyceps sinensis after immersion is fitted into packaging bag, it will be by oxygen and carbon dioxide volume ratio with gas-control packing device
The mixed gas of 65:35 is filled with packaging bag, obtains the cordyceps sinensis of controlled atmospheric packing.
With the cordyceps sinensis of 10MeV electron beam irradiation controlled atmospheric packing, irradiation dose 1.5kGy, unevenness≤1.25.
It is refrigerated at 4 DEG C.
Embodiment 3
At 5 DEG C, with head, stroma and the polypide of the linear flowing water cordyceps sinensis with soil collected to the place of production according to
It is secondary to be cleaned；It is ultrasonic cleaning in 0.5% calcium lactate solution that fresh cordyceps sinensis after cleaning, which is placed in mass percentage concentration,
3 times (power 600W, frequency 80Hz, between 5 DEG C of temperature, time 7min/ times), 5 DEG C drain surface moisture.
Prepare fresh-keeping liquid: the different Vc of 2mmol/L D-sorbite, 2mmol/L, 8 μm of ol/L phytic acid, L-cysteine concentration are
Ethanol extract, 150mg/L chitosan oligosaccharide, the 150mg/L piece coccus antibacterial peptide of 0.6mg/kg, 60mg/mL Cordyceps fermented liquid
(PediocinPA-1, Pediococcus acidilactici P.acidilactici, pediococcus cerevisiae P.cerevisicae, Pediococcus pentosaceus
The Pediococcus species such as P.pentosaceus fermentation liquid).
The preparation of the ethanol extract of Cordyceps fermented liquid: liquid fermentation training is inoculated in after aweto strain is activated
It supports in base (including potato, soya bean, sucrose, glucose, peptone, yeast extract, mineral salt and vitamin), is cultivated at 25 DEG C
7d, centrifuging and taking supernatant, obtains Cordyceps fermented liquid；The volume basis of ethyl alcohol to ethyl alcohol is added into Cordyceps fermented liquid
Concentration stops when being 80%, and supernatant, the ethyl alcohol in vacuum distillation removal supernatant is collected by centrifugation, and freeze-drying obtains winter worm
The ethanol extract of summer grass fermentation liquid.
Fresh cordyceps sinensis after ultrasonic cleaning is impregnated into 3min, 5 DEG C of cold wind dryings, the winter after being impregnated in fresh-keeping liquid
Worm summer grass.Cordyceps sinensis after immersion is fitted into packaging bag, it will be by oxygen and carbon dioxide volume ratio 60 with gas-control packing device:
40 mixed gas is filled with packaging bag, obtains the cordyceps sinensis of controlled atmospheric packing.
With60Co gamma-rays irradiates the cordyceps sinensis of controlled atmospheric packing, irradiation dose 1kGy, unevenness≤1.25.At 2 DEG C
Refrigeration.
Embodiment 4
At 2 DEG C, with head, stroma and the polypide of the linear flowing water cordyceps sinensis with soil collected to the place of production according to
It is secondary to be cleaned；It is ultrasonic cleaning in 0.2% calcium lactate solution that fresh cordyceps sinensis after cleaning, which is placed in mass percentage concentration,
2 times (power 400W, frequency 60Hz, between 2 DEG C of temperature, time 6min/ times), 2 DEG C drain surface moisture.
Prepare fresh-keeping liquid: the different Vc of 2.5mmol/L D-sorbite, 1.5mmol/L, 7.5 μm of ol/L phytic acid, L-cysteines are dense
Degree is that the ethanol extract, 180mg/L chitosan oligosaccharide, 160mg/L streptomycete of 0.5mg/kg, 40mg/mL Cordyceps fermented liquid are anti-
Bacterium peptide (epsilon-polylysine, ε-Polylysine, manufacturer: Zhengzhou Bai Nafo bioengineering limited liability company).
The preparation of the ethanol extract of Cordyceps fermented liquid: liquid fermentation training is inoculated in after aweto strain is activated
It supports in base (including potato, soya bean, sucrose, glucose, peptone, yeast extract, mineral salt and vitamin), is cultivated at 27 DEG C
7d, centrifuging and taking supernatant, obtains Cordyceps fermented liquid；The volume basis of ethyl alcohol to ethyl alcohol is added into Cordyceps fermented liquid
Concentration stops when being 78%, and supernatant, the ethyl alcohol in vacuum distillation removal supernatant is collected by centrifugation, and freeze-drying obtains winter worm
The ethanol extract of summer grass fermentation liquid.
Fresh cordyceps sinensis after ultrasonic cleaning is impregnated into 2min, 5 DEG C of cold wind dryings, the winter after being impregnated in fresh-keeping liquid
Worm summer grass.Cordyceps sinensis after immersion is fitted into packaging bag, it will be by oxygen and carbon dioxide volume ratio 56 with gas-control packing device:
44 mixed gas is filled with packaging bag, obtains the cordyceps sinensis of controlled atmospheric packing.
With the cordyceps sinensis of 5-MeV electron beam irradiation controlled atmospheric packing, irradiation dose 0.8kGy, unevenness≤1.25.
It is refrigerated at 2 DEG C.
Embodiment 5
At 9 DEG C, with head, stroma and the polypide of the linear flowing water cordyceps sinensis with soil collected to the place of production according to
It is secondary to be cleaned；It is in 0.45% calcium lactate solution that fresh cordyceps sinensis after cleaning, which is placed in mass percentage concentration, and ultrasonic wave is clear
It washes 4 times (power 800W, frequency 90Hz, between 4 DEG C of temperature, time 5min/ times), 9 DEG C drain surface moisture.
Prepare fresh-keeping liquid: 2.5mmol/L D-sorbite, 1.5mmol/LVc, 8.5 μm of ol/L phytic acid, L-cysteine concentration
For the ethanol extract of 0.7mg/kg, 50mg/mL Cordyceps fermented liquid, 100mg/L chitosan oligosaccharide, 80mg/L streptomyces antibacterial peptide
(epsilon-polylysine, ε-Polylysine, manufacturer: Zhengzhou Bai Nafo bioengineering limited liability company), 40mg/L lactic acid
Streptococcus antibacterial peptide (Nisin, manufacturer: Shandong Fu Ruida Biotechnology Co., Ltd).
The preparation of the ethanol extract of Cordyceps fermented liquid: liquid fermentation training is inoculated in after aweto strain is activated
It supports in base (including potato, soya bean, sucrose, glucose, peptone, yeast extract, mineral salt and vitamin), is cultivated at 28 DEG C
7d, centrifuging and taking supernatant, obtains Cordyceps fermented liquid；The volume basis of ethyl alcohol to ethyl alcohol is added into Cordyceps fermented liquid
Concentration stops when being 74%, and supernatant, the ethyl alcohol in vacuum distillation removal supernatant is collected by centrifugation, and freeze-drying obtains winter worm
The ethanol extract of summer grass fermentation liquid.
Fresh cordyceps sinensis after ultrasonic cleaning is impregnated into 4min, 4 DEG C of cold wind dryings, the winter after being impregnated in fresh-keeping liquid
Worm summer grass.Cordyceps sinensis after immersion is fitted into packaging bag, it will be by oxygen and carbon dioxide volume ratio 63 with gas-control packing device:
37 mixed gas is filled with packaging bag, obtains the cordyceps sinensis of controlled atmospheric packing.
With60Co gamma-rays irradiates the cordyceps sinensis of controlled atmospheric packing, irradiation dose 0.2kGy, unevenness≤1.25.3
DEG C refrigeration.
Comparative example 1
" storing and fresh-keeping method of fresh cordyceps sinensis " recorded according to Chinese patent 201611222582.3 is to the fresh winter worm summer
Grass carries out fresh-keeping:
The fresh cordyceps sinensis just excavated is taken, is cleaned under 5 DEG C of environment temperatures with 5 DEG C of water respectively, then in 8 DEG C of environment
Under be less than 0.5g, 0.5~1.0g by weight, fall into three classes greater than 1.0g.
Cordyceps sinensis by weight less than 0.5g saves under -5 DEG C, 75% damp condition；
The cordyceps sinensis of 0.5~1.0g of weight is saved under -7 DEG C, 70% damp condition；
Cordyceps sinensis by weight greater than 1.0g saves under -10 DEG C, 60% damp condition.
Embodiment 6
1, process is tested
Take fresh cordyceps sinensis 180 of 0.5~1.0g specification respectively, be randomly divided into 6 groups, respectively according to Examples 1 to 5 and
The method of comparative example 1 is fresh-keeping to the progress of each group fresh cordyceps sinensis, and measurement each group cordyceps sinensis is relative to Preservation Treatment after saving 75d
Preceding weight-loss ratio, soluble protein content rate of descent and polyphenol oxidase activity growth rate.Testing result is as shown in table 1.
Sample weight loss rate is according to " Wang Jingbo waits influence [J] of .3 kind plants essential oil suffocating treatment to nectarine fresh-keeping effect
Nuclear agricultural science report, 2018,32 (5): the method measurement in 933-940. "；Soluble protein content rate of descent is according to " Deng Lili waits
Bright trip's method measurement condition optimizing [J] the Food Science of apple tissue trace soluble egg from content of coomassie, 2012, (24):
185-189. " method measurement；Polyphenol oxidase activity growth rate is according to " Jiankang waits postharvest fruit and vegetable bio-chemical characteristics to instruct
Beijing [M]: China Light Industry Press, the measurement of 2007:59-154. " method.
The results are shown in Table 1, when saving the 75th day, the fresh-keeping cordyceps sinensis of the method for the present invention, in weight-loss ratio, solubility
In terms of protein content rate of descent and polyphenol oxidase activity growth rate, it is fresh-keeping to be superior to 201611222582.3 method of Chinese patent
Cordyceps sinensis.Meanwhile the fresh-keeping effect of the method for the invention is more obvious with the extension advantage of fresh keeping time.
Table 1 saves the 75th day, weight-loss ratio, soluble protein content rate of descent and the polyphenol of each processing group cordyceps sinensis sample
Oxidase active growth rate measurement result
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications are also answered
It is considered as protection scope of the present invention.
Claims (9)
1. a kind of method of the fresh-keeping cordyceps sinensis of irradiation method, comprising the following steps:
(1) fresh cordyceps sinensis is soaked in fresh-keeping liquid, takes out drying, the cordyceps sinensis after being impregnated；
The fresh-keeping liquid includes 1~3mmol/L D-sorbite, 1~3mmol/L vitamin C or ISOASCORBIC ACID, 6~9 μm of ol/L
Phytic acid, the L-cysteine of 0.4~0.8mg/kg, the ethanol extract of 30~100mg/ml Cordyceps fermented liquid, 50~
200mg/L chitosan oligosaccharide and 100~200mg/L antibacterial peptide；
(2) cordyceps sinensis after immersion is packed into packaging bag, is filled with and gas is carried out by the mixed gas that oxygen and carbon dioxide form
It stealthily substitutes dress, obtains the cordyceps sinensis of controlled atmospheric packing；
(3) with60Co gamma-rays or 5~10MeV electron beam irradiate the cordyceps sinensis of controlled atmospheric packing, and irradiation dose is 0.1~
1.5kGy, unevenness≤1.25 refrigerate the cordyceps sinensis after irradiation.
2. the method according to claim 1, wherein the fresh cordyceps sinensis is by ultrasonic cleaning in step (1)
It is soaked in fresh-keeping liquid again.
3. according to the method described in claim 2, it is characterized in that, the power of the ultrasonic cleaning is 250~1200W, ultrasound
The frequency of cleaning is 40kHz~120kHz, and the number of ultrasonic cleaning is 2~5 times, and the time being cleaned by ultrasonic every time independently is 5
~10min.
4. the method according to claim 1, wherein the ethyl alcohol of the Cordyceps fermented liquid mentions in step (1)
Take the preparation method of object the following steps are included:
S1, cordyceps sinensis is inoculated in liquid fermentation medium, cultivates 6~8d, be separated by solid-liquid separation, obtain cordyceps sinensis fermentation
Liquid；
S2, the concentration expressed in percentage by volume that ethyl alcohol to ethyl alcohol are added into Cordyceps fermented liquid reach 70~80%, are centrifuged to obtain supernatant
Liquid removes drying after ethyl alcohol, obtains the ethanol extract of Cordyceps fermented liquid.
5. according to the method described in claim 4, it is characterized in that, the liquid fermentation liquid in the step S1 include potato,
One of soya bean, sucrose, glucose, peptone, yeast extract, mineral salt and vitamin are a variety of.
6. the method according to claim 1, wherein the antibacterial peptide includes that streptococcus lactis is anti-in step (1)
One of bacterium peptide, streptomyces antibacterial peptide and piece coccus antibacterial peptide are a variety of.
7. method according to claim 1 or 6, which is characterized in that the drying temperature in the step (1) is -2~10
℃。
8. the method according to claim 1, wherein oxygen and dioxy in the step (2), in mixed gas
The volume ratio for changing carbon is 55~65:35~45.
9. the method according to claim 1, wherein the refrigerated storage temperature in the step (3) is 0~4 DEG C.
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| CN115957245A (en) * | 2023-01-04 | 2023-04-14 | 西藏挖草人健康产业发展有限公司 | Method for processing cordyceps sinensis preservation lock nutrition through cascade treatment |
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