CN109717347A - A kind of method of controlled atmospheric packing and ultraviolet irradiation composite fresh-keeping cordyceps sinensis - Google Patents
A kind of method of controlled atmospheric packing and ultraviolet irradiation composite fresh-keeping cordyceps sinensis Download PDFInfo
- Publication number
- CN109717347A CN109717347A CN201910175607.6A CN201910175607A CN109717347A CN 109717347 A CN109717347 A CN 109717347A CN 201910175607 A CN201910175607 A CN 201910175607A CN 109717347 A CN109717347 A CN 109717347A
- Authority
- CN
- China
- Prior art keywords
- cordyceps sinensis
- fresh
- liquid
- keeping
- cordyceps
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 241001248610 Ophiocordyceps sinensis Species 0.000 title claims abstract description 171
- 238000000034 method Methods 0.000 title claims abstract description 64
- 238000012856 packing Methods 0.000 title claims abstract description 40
- 239000002131 composite material Substances 0.000 title claims abstract description 13
- 239000007788 liquid Substances 0.000 claims abstract description 120
- 238000001035 drying Methods 0.000 claims abstract description 27
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 57
- 229920001661 Chitosan Polymers 0.000 claims description 44
- 241000190633 Cordyceps Species 0.000 claims description 40
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 30
- 235000019441 ethanol Nutrition 0.000 claims description 29
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Chemical class OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 26
- 239000008103 glucose Chemical class 0.000 claims description 23
- XUJNEKJLAYXESH-REOHCLBHSA-N L-Cysteine Chemical compound SC[C@H](N)C(O)=O XUJNEKJLAYXESH-REOHCLBHSA-N 0.000 claims description 22
- 235000009754 Vitis X bourquina Nutrition 0.000 claims description 22
- 235000012333 Vitis X labruscana Nutrition 0.000 claims description 22
- 235000014787 Vitis vinifera Nutrition 0.000 claims description 22
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims description 21
- 150000001720 carbohydrates Chemical class 0.000 claims description 21
- 239000000469 ethanolic extract Substances 0.000 claims description 20
- 239000006228 supernatant Substances 0.000 claims description 19
- 238000004806 packaging method and process Methods 0.000 claims description 17
- 238000002360 preparation method Methods 0.000 claims description 17
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical group O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 claims description 16
- 238000000855 fermentation Methods 0.000 claims description 16
- 230000004151 fermentation Effects 0.000 claims description 16
- 229960000583 acetic acid Drugs 0.000 claims description 15
- 239000007789 gas Substances 0.000 claims description 15
- 239000012362 glacial acetic acid Substances 0.000 claims description 15
- 238000004506 ultrasonic cleaning Methods 0.000 claims description 15
- 238000004108 freeze drying Methods 0.000 claims description 13
- FBPFZTCFMRRESA-JGWLITMVSA-N D-glucitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-JGWLITMVSA-N 0.000 claims description 12
- IMQLKJBTEOYOSI-GPIVLXJGSA-N Inositol-hexakisphosphate Chemical compound OP(O)(=O)O[C@H]1[C@H](OP(O)(O)=O)[C@@H](OP(O)(O)=O)[C@H](OP(O)(O)=O)[C@H](OP(O)(O)=O)[C@@H]1OP(O)(O)=O IMQLKJBTEOYOSI-GPIVLXJGSA-N 0.000 claims description 12
- IMQLKJBTEOYOSI-UHFFFAOYSA-N Phytic acid Natural products OP(O)(=O)OC1C(OP(O)(O)=O)C(OP(O)(O)=O)C(OP(O)(O)=O)C(OP(O)(O)=O)C1OP(O)(O)=O IMQLKJBTEOYOSI-UHFFFAOYSA-N 0.000 claims description 12
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 12
- 239000001301 oxygen Substances 0.000 claims description 12
- 229910052760 oxygen Inorganic materials 0.000 claims description 12
- 235000002949 phytic acid Nutrition 0.000 claims description 12
- 239000000467 phytic acid Substances 0.000 claims description 12
- 229940068041 phytic acid Drugs 0.000 claims description 12
- 239000004201 L-cysteine Substances 0.000 claims description 11
- 235000013878 L-cysteine Nutrition 0.000 claims description 11
- RQFQJYYMBWVMQG-IXDPLRRUSA-N chitotriose Chemical compound O[C@@H]1[C@@H](N)[C@H](O)O[C@H](CO)[C@H]1O[C@H]1[C@H](N)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O2)N)[C@@H](CO)O1 RQFQJYYMBWVMQG-IXDPLRRUSA-N 0.000 claims description 10
- 238000007654 immersion Methods 0.000 claims description 10
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 claims description 8
- 229930003268 Vitamin C Natural products 0.000 claims description 8
- 235000019154 vitamin C Nutrition 0.000 claims description 8
- 239000011718 vitamin C Substances 0.000 claims description 8
- 238000003860 storage Methods 0.000 claims description 7
- 244000068988 Glycine max Species 0.000 claims description 6
- 235000010469 Glycine max Nutrition 0.000 claims description 6
- 239000001888 Peptone Chemical class 0.000 claims description 6
- 108010080698 Peptones Chemical class 0.000 claims description 6
- 244000061456 Solanum tuberosum Species 0.000 claims description 6
- 235000002595 Solanum tuberosum Nutrition 0.000 claims description 6
- 229930006000 Sucrose Natural products 0.000 claims description 6
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical class O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 6
- 229910052500 inorganic mineral Inorganic materials 0.000 claims description 6
- 239000011707 mineral Chemical class 0.000 claims description 6
- 235000019319 peptone Nutrition 0.000 claims description 6
- 150000003839 salts Chemical class 0.000 claims description 6
- 239000005720 sucrose Substances 0.000 claims description 6
- 229940088594 vitamin Drugs 0.000 claims description 6
- 229930003231 vitamin Natural products 0.000 claims description 6
- 235000013343 vitamin Nutrition 0.000 claims description 6
- 239000011782 vitamin Substances 0.000 claims description 6
- 150000003722 vitamin derivatives Chemical class 0.000 claims description 6
- CIWBSHSKHKDKBQ-DUZGATOHSA-N D-isoascorbic acid Chemical compound OC[C@@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-DUZGATOHSA-N 0.000 claims description 5
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical class OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims description 5
- 229940041514 candida albicans extract Drugs 0.000 claims description 5
- 235000010350 erythorbic acid Nutrition 0.000 claims description 5
- 229940026239 isoascorbic acid Drugs 0.000 claims description 5
- 238000005057 refrigeration Methods 0.000 claims description 5
- 238000002604 ultrasonography Methods 0.000 claims description 5
- 239000012138 yeast extract Chemical class 0.000 claims description 5
- 238000002791 soaking Methods 0.000 claims description 4
- 238000004320 controlled atmosphere Methods 0.000 claims description 3
- 238000000926 separation method Methods 0.000 claims description 3
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 2
- 229910052799 carbon Inorganic materials 0.000 claims description 2
- 240000006365 Vitis vinifera Species 0.000 claims 3
- GWEVSGVZZGPLCZ-UHFFFAOYSA-N Titan oxide Chemical compound O=[Ti]=O GWEVSGVZZGPLCZ-UHFFFAOYSA-N 0.000 claims 2
- 239000004408 titanium dioxide Substances 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 24
- 238000004321 preservation Methods 0.000 abstract description 22
- 208000016261 weight loss Diseases 0.000 abstract description 10
- 230000004580 weight loss Effects 0.000 abstract description 10
- 102000030523 Catechol oxidase Human genes 0.000 abstract description 9
- 108010031396 Catechol oxidase Proteins 0.000 abstract description 9
- 230000007423 decrease Effects 0.000 abstract description 8
- 230000003111 delayed effect Effects 0.000 abstract description 2
- 241000219095 Vitis Species 0.000 description 19
- 238000004140 cleaning Methods 0.000 description 17
- 239000000243 solution Substances 0.000 description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 14
- 241001018099 Alloteropsis Species 0.000 description 10
- 235000017898 Digitaria ciliaris Nutrition 0.000 description 10
- 238000005119 centrifugation Methods 0.000 description 8
- 239000003795 chemical substances by application Substances 0.000 description 8
- 238000004519 manufacturing process Methods 0.000 description 8
- 239000000463 material Substances 0.000 description 8
- MKJXYGKVIBWPFZ-UHFFFAOYSA-L calcium lactate Chemical compound [Ca+2].CC(O)C([O-])=O.CC(O)C([O-])=O MKJXYGKVIBWPFZ-UHFFFAOYSA-L 0.000 description 7
- 239000001527 calcium lactate Substances 0.000 description 7
- 229960002401 calcium lactate Drugs 0.000 description 7
- 235000011086 calcium lactate Nutrition 0.000 description 7
- 229910002092 carbon dioxide Inorganic materials 0.000 description 7
- 229960004424 carbon dioxide Drugs 0.000 description 7
- 239000003963 antioxidant agent Substances 0.000 description 6
- 230000003078 antioxidant effect Effects 0.000 description 6
- 235000006708 antioxidants Nutrition 0.000 description 6
- 239000001569 carbon dioxide Substances 0.000 description 6
- 239000000284 extract Substances 0.000 description 6
- 238000005259 measurement Methods 0.000 description 6
- 230000008569 process Effects 0.000 description 6
- 238000012545 processing Methods 0.000 description 6
- 239000000047 product Substances 0.000 description 6
- 206010013786 Dry skin Diseases 0.000 description 5
- 235000010755 mineral Nutrition 0.000 description 5
- 239000011259 mixed solution Substances 0.000 description 5
- 235000016709 nutrition Nutrition 0.000 description 5
- 230000003647 oxidation Effects 0.000 description 5
- 238000007254 oxidation reaction Methods 0.000 description 5
- 239000000376 reactant Substances 0.000 description 5
- 239000002689 soil Substances 0.000 description 5
- 241000196324 Embryophyta Species 0.000 description 4
- 210000004027 cell Anatomy 0.000 description 4
- 239000000470 constituent Substances 0.000 description 4
- 239000000796 flavoring agent Substances 0.000 description 4
- 235000019634 flavors Nutrition 0.000 description 4
- -1 glucose Compound Chemical class 0.000 description 4
- 230000035764 nutrition Effects 0.000 description 4
- 230000004224 protection Effects 0.000 description 4
- 102000004169 proteins and genes Human genes 0.000 description 4
- 108090000623 proteins and genes Proteins 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- 235000001674 Agaricus brunnescens Nutrition 0.000 description 3
- 239000002253 acid Substances 0.000 description 3
- 238000007605 air drying Methods 0.000 description 3
- 230000000844 anti-bacterial effect Effects 0.000 description 3
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 3
- 230000006378 damage Effects 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 235000013305 food Nutrition 0.000 description 3
- 238000007710 freezing Methods 0.000 description 3
- 235000012055 fruits and vegetables Nutrition 0.000 description 3
- 230000006870 function Effects 0.000 description 3
- 235000001727 glucose Nutrition 0.000 description 3
- 150000004676 glycans Chemical class 0.000 description 3
- 239000010410 layer Substances 0.000 description 3
- 229920001282 polysaccharide Polymers 0.000 description 3
- 239000005017 polysaccharide Substances 0.000 description 3
- 239000007787 solid Substances 0.000 description 3
- 230000001954 sterilising effect Effects 0.000 description 3
- 238000004659 sterilization and disinfection Methods 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 238000012549 training Methods 0.000 description 3
- 238000005292 vacuum distillation Methods 0.000 description 3
- LRFVTYWOQMYALW-UHFFFAOYSA-N 9H-xanthine Chemical compound O=C1NC(=O)NC2=C1NC=N2 LRFVTYWOQMYALW-UHFFFAOYSA-N 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 2
- XKRFYHLGVUSROY-UHFFFAOYSA-N Argon Chemical compound [Ar] XKRFYHLGVUSROY-UHFFFAOYSA-N 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 2
- 244000025254 Cannabis sativa Species 0.000 description 2
- VGGSQFUCUMXWEO-UHFFFAOYSA-N Ethene Chemical compound C=C VGGSQFUCUMXWEO-UHFFFAOYSA-N 0.000 description 2
- SQUHHTBVTRBESD-UHFFFAOYSA-N Hexa-Ac-myo-Inositol Natural products CC(=O)OC1C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C1OC(C)=O SQUHHTBVTRBESD-UHFFFAOYSA-N 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- OIRDTQYFTABQOQ-KQYNXXCUSA-N adenosine Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O OIRDTQYFTABQOQ-KQYNXXCUSA-N 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 230000003712 anti-aging effect Effects 0.000 description 2
- 230000000845 anti-microbial effect Effects 0.000 description 2
- 230000000259 anti-tumor effect Effects 0.000 description 2
- 230000033228 biological regulation Effects 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 238000004821 distillation Methods 0.000 description 2
- 230000002255 enzymatic effect Effects 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- UYTPUPDQBNUYGX-UHFFFAOYSA-N guanine Chemical compound O=C1NC(N)=NC2=C1N=CN2 UYTPUPDQBNUYGX-UHFFFAOYSA-N 0.000 description 2
- FDGQSTZJBFJUBT-UHFFFAOYSA-N hypoxanthine Chemical compound O=C1NC=NC2=C1NC=N2 FDGQSTZJBFJUBT-UHFFFAOYSA-N 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 239000012535 impurity Substances 0.000 description 2
- 230000002401 inhibitory effect Effects 0.000 description 2
- CDAISMWEOUEBRE-GPIVLXJGSA-N inositol Chemical group O[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@H](O)[C@@H]1O CDAISMWEOUEBRE-GPIVLXJGSA-N 0.000 description 2
- 229960000367 inositol Drugs 0.000 description 2
- 229920001684 low density polyethylene Polymers 0.000 description 2
- 239000004702 low-density polyethylene Substances 0.000 description 2
- 230000004060 metabolic process Effects 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 210000002686 mushroom body Anatomy 0.000 description 2
- 239000003755 preservative agent Substances 0.000 description 2
- 230000002335 preservative effect Effects 0.000 description 2
- 230000001681 protective effect Effects 0.000 description 2
- 150000003254 radicals Chemical class 0.000 description 2
- 230000000241 respiratory effect Effects 0.000 description 2
- 230000029058 respiratory gaseous exchange Effects 0.000 description 2
- CDAISMWEOUEBRE-UHFFFAOYSA-N scyllo-inosotol Natural products OC1C(O)C(O)C(O)C(O)C1O CDAISMWEOUEBRE-UHFFFAOYSA-N 0.000 description 2
- UHDGCWIWMRVCDJ-UHFFFAOYSA-N 1-beta-D-Xylofuranosyl-NH-Cytosine Natural products O=C1N=C(N)C=CN1C1C(O)C(O)C(CO)O1 UHDGCWIWMRVCDJ-UHFFFAOYSA-N 0.000 description 1
- 239000002126 C01EB10 - Adenosine Substances 0.000 description 1
- UHDGCWIWMRVCDJ-PSQAKQOGSA-N Cytidine Natural products O=C1N=C(N)C=CN1[C@@H]1[C@@H](O)[C@@H](O)[C@H](CO)O1 UHDGCWIWMRVCDJ-PSQAKQOGSA-N 0.000 description 1
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 1
- MYMOFIZGZYHOMD-UHFFFAOYSA-N Dioxygen Chemical compound O=O MYMOFIZGZYHOMD-UHFFFAOYSA-N 0.000 description 1
- 241000790917 Dioxys <bee> Species 0.000 description 1
- 208000035240 Disease Resistance Diseases 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- UGQMRVRMYYASKQ-UHFFFAOYSA-N Hypoxanthine nucleoside Natural products OC1C(O)C(CO)OC1N1C(NC=NC2=O)=C2N=C1 UGQMRVRMYYASKQ-UHFFFAOYSA-N 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 102000004316 Oxidoreductases Human genes 0.000 description 1
- 108090000854 Oxidoreductases Proteins 0.000 description 1
- CBENFWSGALASAD-UHFFFAOYSA-N Ozone Chemical compound [O-][O+]=O CBENFWSGALASAD-UHFFFAOYSA-N 0.000 description 1
- 235000006029 Prunus persica var nucipersica Nutrition 0.000 description 1
- 244000017714 Prunus persica var. nucipersica Species 0.000 description 1
- 229930182558 Sterol Natural products 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000001133 acceleration Effects 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000011149 active material Substances 0.000 description 1
- 229960005305 adenosine Drugs 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 238000004378 air conditioning Methods 0.000 description 1
- 230000001476 alcoholic effect Effects 0.000 description 1
- 230000003064 anti-oxidating effect Effects 0.000 description 1
- 230000003260 anti-sepsis Effects 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 229910052786 argon Inorganic materials 0.000 description 1
- 239000012298 atmosphere Substances 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- POIARNZEYGURDG-FNORWQNLSA-N beta-damascenone Chemical compound C\C=C\C(=O)C1=C(C)C=CCC1(C)C POIARNZEYGURDG-FNORWQNLSA-N 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 229910002090 carbon oxide Inorganic materials 0.000 description 1
- 210000004413 cardiac myocyte Anatomy 0.000 description 1
- 230000009084 cardiovascular function Effects 0.000 description 1
- 230000005779 cell damage Effects 0.000 description 1
- 239000002738 chelating agent Substances 0.000 description 1
- 239000012459 cleaning agent Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 239000006071 cream Substances 0.000 description 1
- UHDGCWIWMRVCDJ-ZAKLUEHWSA-N cytidine Chemical compound O=C1N=C(N)C=CN1[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O1 UHDGCWIWMRVCDJ-ZAKLUEHWSA-N 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000001934 delay Effects 0.000 description 1
- 238000000151 deposition Methods 0.000 description 1
- 230000006866 deterioration Effects 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000004945 emulsification Methods 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 239000005452 food preservative Substances 0.000 description 1
- 235000019249 food preservative Nutrition 0.000 description 1
- 210000005096 hematological system Anatomy 0.000 description 1
- 125000004435 hydrogen atom Chemical group [H]* 0.000 description 1
- 239000000413 hydrolysate Substances 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 210000000987 immune system Anatomy 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 230000002147 killing effect Effects 0.000 description 1
- 230000000670 limiting effect Effects 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 230000010534 mechanism of action Effects 0.000 description 1
- 229910021645 metal ion Inorganic materials 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 230000001473 noxious effect Effects 0.000 description 1
- 239000002777 nucleoside Substances 0.000 description 1
- 125000003835 nucleoside group Chemical group 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 238000002161 passivation Methods 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 231100000719 pollutant Toxicity 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 150000008442 polyphenolic compounds Chemical class 0.000 description 1
- 235000013824 polyphenols Nutrition 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 239000003223 protective agent Substances 0.000 description 1
- 239000011241 protective layer Substances 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000002829 reductive effect Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 210000002345 respiratory system Anatomy 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 210000000697 sensory organ Anatomy 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- PPASLZSBLFJQEF-RKJRWTFHSA-M sodium erythorbate Chemical compound [Na+].OC[C@@H](O)[C@H]1OC(=O)C(O)=C1[O-] PPASLZSBLFJQEF-RKJRWTFHSA-M 0.000 description 1
- 235000010352 sodium erythorbate Nutrition 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 150000003432 sterols Chemical class 0.000 description 1
- 235000003702 sterols Nutrition 0.000 description 1
- 235000000346 sugar Nutrition 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 239000008399 tap water Substances 0.000 description 1
- 235000020679 tap water Nutrition 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 238000009281 ultraviolet germicidal irradiation Methods 0.000 description 1
- 210000005167 vascular cell Anatomy 0.000 description 1
- 239000000341 volatile oil Substances 0.000 description 1
- 235000020795 whole food diet Nutrition 0.000 description 1
- 229940075420 xanthine Drugs 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/90—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in food processing or handling, e.g. food conservation
Landscapes
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The present invention provides the method for a kind of controlled atmospheric packing and ultraviolet irradiation composite fresh-keeping cordyceps sinensis; it is related to cordyceps sinensis technical field of preservation of fresh; the method of controlled atmospheric packing and ultraviolet irradiation composite fresh-keeping cordyceps sinensis of the present invention; first fresh cordyceps sinensis is impregnated in fresh-keeping liquid; it is soaked in overlay film liquid again after taking out drying; show to form layer protecting film in cordyceps sinensis, ultraviolet irradiation will be carried out after cordyceps sinensis controlled atmospheric packing coated with film, realizes to the fresh-keeping of cordyceps sinensis.The present invention is fresh-keeping using fresh-keeping liquid, by the way of overlay film liquid overlay film, controlled atmospheric packing and ultraviolet irradiation integrated treatment, the weight-loss ratio decline of cordyceps sinensis can be significantly inhibited, slows down the decline of soluble protein content and polyphenol oxidase activity is delayed to increase, relative to conventional preservation method, fresh keeping time at least can achieve 75d, keep better effect to the color, smell and taste of fresh cordyceps sinensis.
Description
Technical field
The present invention relates to cordyceps sinensis technical field of preservation of fresh more particularly to a kind of controlled atmospheric packings and ultraviolet irradiation composite fresh-keeping
The method of cordyceps sinensis.
Background technique
Cordyceps sinensis is a kind of high-grade nourishing rare traditional Chinese medicine, nutriment and active constituent containing there are many, including nucleosides,
Polysaccharide, sterol, fatty acid, protein, polypeptide and volatile component etc. have antitumor, adjusting immune system, promotion body thin
Cellular damage reparation, free radical resisting, anti-oxidant, anti-aging, protection cardiac muscle and vascular cell improve cardiovascular function, protection liver
Dirty, protection kidney adjusts respiratory system, adjusts hematological system and adjusts the functions such as blood lipid.
Cordyceps sinensis on the market is dry cordyceps sinensis, a large amount of volatile components in cordyceps sinensis drying process mostly at present
It can evaporate, part nutrition can be lost, and active constituent can inactivate, while being taken mouthfeel and being greatly reduced, and fresh and alive cordyceps sinensis can be most
Its nutriment of the reservation of limits and active constituent, and keep its unique faint scent and crispy texture.In addition, pharmacology
Studies have shown that fresh cordyceps sinensis is in the pharmacological actions such as immunological regulation, antitumor, anti-oxidant compared with dry cordyceps sinensis
Aspect all has better effects.Therefore, the fresh-keeping of fresh cordyceps sinensis is urgently to be solved in the process of manufacture faced at present
Most significant problems.
Preservation method about fresh cordyceps sinensis is one of the hot spot direction of cordyceps sinensis research in recent years, studies main collection
In preservation by low temperature, quick-freezing fresh-keeping, in terms of, related patents method is as follows:
((CN 102406164A) discloses a kind of fresh and alive cordyceps sinensis processing and its can preparation side to Chinese patent application
Method fresh cordyceps sinensis will be mainly classified after cleaning in -70 DEG C of stored frozens of ultralow temperature, rear thawed by cordyceps sinensis,
It is immersed in the tap water fresh-keeping liquid containing citric acid, sodium isoascorbate, is examined, high temperature sterilization is prepared into can.
Chinese invention patent (CN 1037405928) discloses a kind of preservation method of cordyceps sinensis, to cordyceps sinensis
Head, after stroma and the cleaning of polypide branch, nutriment and active material are lost in order to prevent, by the fresh winter worm summer after cleaning
Grass, which is placed in 0.9% acidic sodium chloride solution, impregnates 5-8min: quickly cooling handles cordyceps sinensis 5-12h under the conditions of -75 DEG C again
Afterwards, it is placed in -5~-25 DEG C of vacuum cold case and saves.
Chinese invention patent (CN 1021782268) discloses a kind of fresh processing technique of cordyceps sinensis, mainly will
Fresh cordyceps sinensis deepfreeze under conditions of being lower than -65 DEG C: while mode cleaning and sterilization of the ultrasonic wave in conjunction with ozone is set,
It is saved at last 0 DEG C.
Chinese patent application (CN 105199956A) discloses a kind of preservation method of cordyceps sinensis, mainly uses one
After determining the cleaning of concentration alcoholic solution, being sprayed on cordyceps sinensis surface with hydromel saves nutrient water from damage, then carries out air-drying dehumidifying
Processing, then quick-frozen at one 30 DEG C be placed on -5 DEG C of cold storage warehouses below and stored.
Chinese invention patent (CN 106727734A) discloses a kind of fresh cordyceps sinensis product and preparation method thereof, mainly
It is by fresh cordyceps sinensis using flowing water and after being cleaned by ultrasonic, atomizing humidifying performance handles 10-40min, then uses 3-7%O2,75-
92%N2And 5-18%CO2Controlled atmospheric packing is carried out with fresh cordyceps sinensis is compared, the cordycepic acid content of fresh cordyceps sinensis product is not low
In 2%, polysaccharide is not less than 0.5%.
Chinese invention patent (CN 106720232A) discloses a kind of storing and fresh-keeping method of fresh cordyceps sinensis, mainly
The methods of saved using Low-temperature cleaning, low temperature classification, classification, entire technique carries out at low ambient temperatures, makes fresh cordyceps sinensis
Fresh keeping time greatly prolongs, and reduces the loss of effective component, while reducing fresh-keeping cost.
Some fresh-keeping thinkings of the above prior art are saved in such a way that direct superfreeze is situated between 70 DEG C, Yi Xieshi
(0 DEG C or less) of low temperature preservation after the quick-frozen latching effective component of ultralow temperature (- 30 DEG C to -75 DEG C), also some technologies are sought to save from damage
It supports moisture or keeps color, increase the step of impregnating sour water or sprinkling hydromel, these prior arts provide fresh winter worm
Summer grass, compares dry cordyceps sinensis product and remains most nutriment and active constituent, but above-mentioned preservation method is big
Mostly using sense organ method from appearance color, smell, hardness, mouthfeel, flavour etc. variation aspect judgement sample freshness, it is subjective because
Element interference greatly, not can effectively solve loss of water in preservation process, soluble protein content decline, polyphenol oxidase enzyme activity still
Property increase etc. caused by cordyceps sinensis freshness decline the problem of.
The water content of the cordyceps sinensis of fresh acquisition is higher, and in storage period, its moisture content factor solid object surface steams
It rises and acts on and scatter and disappear, weightlessness can cause the symptoms such as sample softening physiology that its freshness is caused to decline.Soluble protein participates in
The regulation of a variety of Physiological and Biochemical Metabolism processes of edible mushroom is one of the important indicator of edible mushroom quality and nutrition.Under its content
Drop is considered as adopting an important feature of rear sample aging, with the extension of storage time, due to lacking in nitrogen source and mushroom body
The enhancing of prolease activity, accelerates the decomposition of protein, to change the flavor of mushroom body.Polyphenol oxidase is edible
The main enzyme of bacterium enzymatic browning can be such that product brown stain even ruins, the serious nutritive value for damaging edible mushroom, flavor and outer
Texture is seen, polyphenol oxidase activity is lower to illustrate that storage property can be better.
Currently, being concentrated mainly on preservation by low temperature, quick-freezing fresh-keeping, controlled atmosphere for the research of cordyceps sinensis store and processing
Deng.The principle of cordyceps sinensis preservation by low temperature is mainly the respiration for inhibiting living cells, the metabolism for reducing cell, and low temperature is protected
The temperature for depositing cordyceps sinensis is normally controlled in -3~-10 DEG C.The temperature of quick-freezing fresh-keeping is normally controlled in -80 DEG C.Controlled atmosphere
Principle is to obtain the regulating gas different from normal atmosphere component, suppression by various regulative modes in certain closed system
System leads to the activity of corrupt physiological and biochemical procedure and microorganism.In addition, deepening continuously with the fresh-keeping research of fresh cordyceps sinensis, mesh
The generation of the preceding intelligent fresh-keeping system for having had been reported that fresh cordyceps sinensis is simultaneously applied in practice, mainly passes through intelligent program
Regulate and control cold storing and fresh-keeping temperature, time, the parameters such as humidity, from cell level keep fresh cordyceps sinensis fresh vitality and nutrition at
Point.But above-mentioned preservation technology is higher to the dependence of freshness preserving equipment, operation is relatively complicated, and time-consuming, and one-time investment is big,
It is not easy to popularize.
The present invention carries out the Preservation Treatment of fresh cordyceps sinensis using ultraviolet irradiation composite fresh-keeping method.Short wave ultraviolet UV-C
Wavelength be less than 280nm, be a kind of safely and effectively surface cold sterilization technology using UV-C irradiation, the micro- life of fruit and vegetable surfaces can be killed
Object, passivation enzymatic activity.Keyser et al. (2008) will not generate noxious material or bright studies have shown that ultraviolet irradiation noresidue
Aobvious avirulent by-product, nutrition is pursued in the production and consumer for meeting current green food, safety, wholefood become
Gesture, therefore, UV-C irradiation in recent years receive more and more attention in terms of preserving fruit and vegetable utilizing.Related UV-C control disease of rotting
The mechanism of action oneself through having conducted extensive research.The control corruption effect of UV-C mainly passes through three aspect effects and realizes: one, directly killing
Bacterium；Two, induction plant generates disease resistance；Three, the generation of plants antimicrobial substance and protective plant protecting agent is induced.But so far there are no applies
UV-C irradiation technique animal this to cordyceps sinensis-plant complex carries out effectively fresh-keeping report.
Summary of the invention
Existing fresh cordyceps sinensis preservation method is short for cordyceps sinensis fresh keeping time, relies on equipment in order to overcome by the present invention
The high defect of property, provides the method for a kind of controlled atmospheric packing and ultraviolet irradiation composite fresh-keeping cordyceps sinensis, small to device dependence,
Easy to operate, fresh cordyceps sinensis quality fresh-keeping for a long time is good.
In order to achieve the above-mentioned object of the invention, the present invention the following technical schemes are provided:
The present invention provides a kind of methods of controlled atmospheric packing and ultraviolet irradiation composite fresh-keeping cordyceps sinensis, including following step
It is rapid:
(1) fresh cordyceps sinensis is soaked in fresh-keeping liquid, takes out drying, the cordyceps sinensis after being impregnated；
The fresh-keeping liquid includes 1~3mmol/L D-sorbite, 1~3mmol/L vitamin C or ISOASCORBIC ACID, 6~9 μ
The L-cysteine concentration of mol/L phytic acid, 0.4~0.8mg/kg；
(2) cordyceps sinensis after immersion is soaked in overlay film liquid, takes out drying, obtains the cordyceps sinensis of overlay film；
The overlay film liquid includes the ethanol extract of 30~100mg/ml Cordyceps fermented liquid, 5~30mg/L chitosan oligosaccharide
With 5~50mg/L chitosan grape saccharide complex；
(3) cordyceps sinensis of overlay film is packed into packaging bag, is filled with and is carried out by the mixed gas that oxygen and carbon dioxide form
Controlled atmospheric packing obtains the cordyceps sinensis of controlled atmospheric packing；
(4) ultraviolet irradiation, refrigeration are carried out to the cordyceps sinensis of controlled atmospheric packing；
The irradiation dose is 2~4KJ/m2, irradiation time is 10~20min.
Preferably, in step (1), the fresh cordyceps sinensis is soaked in fresh-keeping liquid again by ultrasonic cleaning.
Preferably, the clear power of the ultrasound is 250~1200W, and the frequency of ultrasonic cleaning is 40kHz~120kHz, is surpassed
The number of sound cleaning is 2~5 times, and the time being cleaned by ultrasonic every time independently is 5~10min.
Preferably, in step (1), the preparation method of the ethanol extract of the Cordyceps fermented liquid includes following step
It is rapid:
S1, cordyceps sinensis is inoculated in liquid fermentation medium, cultivates 6~8d, be separated by solid-liquid separation, obtain cordyceps sinensis hair
Zymotic fluid；
S2, the concentration expressed in percentage by volume that ethyl alcohol to ethyl alcohol are added into Cordyceps fermented liquid reach 70~80%, are centrifuged
Supernatant removes drying after ethyl alcohol, obtains the ethanol extract of Cordyceps fermented liquid.
Preferably, the liquid fermentation liquid in the step S1 includes potato, soya bean, sucrose, glucose, peptone, ferment
One of female cream, mineral salt and vitamin are a variety of.
Preferably, in step (1), the preparation method of the chitosan grape saccharide complex the following steps are included:
A1, chitosan and glucose are dissolved in glacial acetic acid, are configured to containing 0.5~2% chitosan of volumetric concentration and body
The glacial acetic acid solution of product 0.5~2% glucose of concentration；
A2, the glacial acetic acid solution containing 0.5~2% glucose of 0.5~2% chitosan of volumetric concentration and volumetric concentration is existed
103.4KPa, 15~20min is reacted under conditions of 121.3 DEG C, freeze-drying obtains chitosan grape saccharide complex.
Preferably, the soaking time in the step (1) is 2~5min.
Preferably, the soaking time in the step (2) is 30~200s.
Preferably, in the step (3), the volume ratio of oxygen and carbon dioxide is 55~65:35-45 in mixed gas.
Preferably, the refrigerated storage temperature in the step (4) is -4~4 DEG C.
Compared with prior art, beneficial effects of the present invention:
The present invention provides a kind of methods of controlled atmospheric packing and ultraviolet irradiation composite fresh-keeping cordyceps sinensis, first by the fresh winter worm summer
Grass impregnates in fresh-keeping liquid, is soaked in overlay film liquid again after taking out drying, shows to form layer protecting film in cordyceps sinensis, will cover
Ultraviolet irradiation is carried out after cordyceps sinensis controlled atmospheric packing after film, is realized to the fresh-keeping of cordyceps sinensis.The present invention, which utilizes, contains argon gas
Mixed gas carry out controlled atmospheric packing, can significantly inhibit cordyceps sinensis weight-loss ratio decline, slow down soluble protein content
Decline and polyphenol oxidase activity is delayed to increase, relative to conventional preservation method, fresh keeping time at least can achieve 75d, right
The color, smell and taste of fresh cordyceps sinensis keep better effect.
Specific embodiment
The present invention provides a kind of methods of controlled atmospheric packing and ultraviolet irradiation composite fresh-keeping cordyceps sinensis, including following step
It is rapid:
(1) fresh cordyceps sinensis is soaked in fresh-keeping liquid, takes out drying, the cordyceps sinensis after being impregnated；
The fresh-keeping liquid includes 1~3mmol/L D-sorbite, 1~3mmol/L vitamin C or ISOASCORBIC ACID, 6~9 μ
The L-cysteine concentration of mol/L phytic acid, 0.4~0.8mg/kg；
(2) cordyceps sinensis after immersion is soaked in overlay film liquid, takes out drying, obtains the cordyceps sinensis of overlay film；
The overlay film liquid includes the ethanol extract of 30~100mg/ml Cordyceps fermented liquid, 5~30mg/L chitosan oligosaccharide
With 5~50mg/L chitosan grape saccharide complex；
(3) cordyceps sinensis of overlay film is packed into packaging bag, is filled with and is carried out by the mixed gas that oxygen and carbon dioxide form
Controlled atmospheric packing obtains the cordyceps sinensis of controlled atmospheric packing；
(4) ultraviolet irradiation, refrigeration are carried out to the cordyceps sinensis of controlled atmospheric packing；
The irradiation dose is 2~4KJ/m2, irradiation time is 10~20min.
Fresh cordyceps sinensis is soaked in fresh-keeping liquid by the present invention, takes out drying, the cordyceps sinensis after being impregnated.The present invention
Fresh-keeping liquid is prepared to impregnate fresh cordyceps sinensis, to keep the moisture of fresh cordyceps sinensis in preservation process, prevent oxidation, etc. originals
The brown stain because caused by and putrid and deteriorated reduces weight-loss ratio, prevents from softening.Fresh-keeping liquid of the present invention include composite antioxidant and
Preservation is played in compound preservative, the limitation for avoiding the fresh-keeping approach of single antistaling agent single, collaboration.Each original in fresh-keeping liquid
The composition ratio of material is the active function range of component, and controls and add within acceptable dose range in food.
The fresh-keeping liquid that the present invention uses fresh cordyceps sinensis of the present invention refers to that acquisition time is no more than for 24 hours and does not do
Dry cordyceps sinensis.Preservation method of the present invention is to protect the quality of fresh cordyceps sinensis not significantly change.
Fresh cordyceps sinensis of the present invention is preferably cleaned before being soaked in fresh-keeping liquid, and the cleaning agent of the cleaning is excellent
Choosing includes water or calcium lactate solution.The purpose that the present invention cleans fresh cordyceps sinensis be in order to remove the impurity such as silt, not into
Row cleaning can also realize the fresh-keeping effect of preservation method of the present invention.In the present invention, the quality hundred of the calcium lactate solution
Point concentration is preferably 0.1~0.5%, and more preferably 0.2~0.4%.
In the present invention, the method for the cleaning is preferably cleaned by ultrasonic, using ultrasonic wave cavitation in a liquid,
Acceleration effect and direct flow effect to liquid and dirt directly, indirectly effect, make crud layer by dispersion, emulsification, removing and
Reach and rapidly and efficiently clean fresh cordyceps sinensis surface risk pollutant purpose, Conventional cleaning methods is avoided to damage in the process of cleaning
Sample surfaces.Currently preferred, the power of the ultrasonic cleaning is 250~1200W；More preferably 600~900W.The present invention
Preferably, the frequency of the ultrasonic cleaning is preferably 40kHz~120kHz；More preferably 70~80kHz.It is currently preferred,
The number of the ultrasonic cleaning is preferably 2~5 times, and more preferably 3 times；The present invention is it is further preferred that be every time cleaned by ultrasonic
Time independently is 5~10min, is still more preferably 6~8min.
In the present invention, the fresh-keeping liquid includes that 1~3mmol/L D-sorbite, 1~3mmol/L vitamin C or different dimension are raw
The L-cysteine of plain C, 6~9 μm of ol/L phytic acid, 0.4~0.8mg/kg；It preferably include 1.5~2mmol/L D-sorbite, 2
The L-cysteine of~2.5mmol/L vitamin C or ISOASCORBIC ACID, 7~8 μm of ol/L phytic acid, 0.5~0.6mg/kg.
In the present invention, D-sorbite has the function of moisture absorption, water conservation, the D-sorbite in fresh-keeping liquid of the present invention
It is used as water-retaining agent.
In the present invention, the vitamin C or ISOASCORBIC ACID use in fresh-keeping liquid as antioxidant, can keep fresh
The color and natural flavor of cordyceps sinensis, prolong storage period, and without any side effects.
In the present invention, phytic acid uses in fresh-keeping liquid as antioxidant, metal ion chelation agent and antistaling agent.Phytic acid
Hydrolysate is inositol and phosphatide in human body, and inositol has anti-aging effect, and phosphatide is the important component of human body cell.
Meanwhile each matter acid molecule can provide six pairs of hydrogen atoms, make free radical electronically forms rock-steady structure, thus instead of by fresh-keeping
Cordyceps sinensis is used as oxygen molecule, avoids fresh cordyceps sinensis oxidation deterioration.
In the present invention, L-cysteine uses in fresh-keeping liquid as BPH resistant rice variety antistaling agent, to prevent vitamin C or different
Vitamin C oxidation, can also prevent the brown stain of cordyceps sinensis surface.
In the present invention, the time that the fresh cordyceps sinensis impregnates in fresh-keeping liquid is preferably 2~5min, and more preferably 3
~4min.The present invention is not particularly limited the dosage of the fresh-keeping liquid, can submerge fresh cordyceps sinensis.
In the present invention, the temperature of the drying is preferably 0~4 DEG C, and more preferably 2~3 DEG C.The present invention selects 0~4 DEG C
Be dried is in order to avoid causing the corruption of cordyceps sinensis to accelerate and internal moisture loss and nutritional ingredient, heat because of high temperature
The loss of quick property substance.It is not particularly limited in mode of the present invention to the drying, using methods known in the art, example
Such as cold air drying.
After cordyceps sinensis after being impregnated, the cordyceps sinensis after immersion is soaked in overlay film liquid by the present invention, is taken out dry
It is dry, obtain the cordyceps sinensis of overlay film.Overlay film liquid of the present invention can form one layer of antibacterial, oxidation resistant on cordyceps sinensis surface
Protective layer, in addition to can prevent the brown stains caused by reasons such as oxidation, spoilage organisms proliferation and it is putrid and deteriorated while, can also be effectively
The raising for inhibiting its respiratory intensity delays the decline of the nutriments such as soluble solid and titratable acid, reduces weight-loss ratio, prevents
Only soften.
Overlay film liquid of the present invention includes the ethanol extract of 30~100mg/ml Cordyceps fermented liquid, 5~30mg/L
Chitosan oligosaccharide and 5~50mg/L chitosan grape saccharide complex；Preferred includes the ethyl alcohol of 50~80mg/ml Cordyceps fermented liquid
Extract, 10~20mg/L chitosan oligosaccharide and 15~35mg/L chitosan grape saccharide complex.
In the present invention, the principle active component in the ethanol extract of the Cordyceps fermented liquid is guanine, worm
Careless element, hypoxanthine, cytidine, xanthine and adenosine, rise in overlay film liquid of the present invention and use as antimicrobial antistaling agent, inhibit
Bacterial reproduction in preservation process.
Specifically, the ethanol extract of Cordyceps fermented liquid of the present invention is preferably according to the side included the following steps
It is prepared by method:
S1, cordyceps sinensis is inoculated in liquid fermentation medium, cultivates 6~8d, be separated by solid-liquid separation, obtain cordyceps sinensis hair
Zymotic fluid；
S2, the concentration expressed in percentage by volume that ethyl alcohol to ethyl alcohol are added into Cordyceps fermented liquid reach 70~80%, are centrifuged
Supernatant removes drying after ethyl alcohol, obtains the ethanol extract of Cordyceps fermented liquid.
In the present invention, the liquid fermentation medium preferably includes but is not limited to potato, soya bean, sucrose, grape
One of sugar, peptone, yeast extract, mineral salt and vitamin are a variety of.In the present invention, the cordyceps sinensis is inoculated with liquid
The inoculum concentration of fermentation medium is preferably 0.01~0.1g/ml, more preferably 0.05g/ml.In the present invention, the culture
Time is preferably 6~8d, more preferably 7d.In the present invention, the temperature of the culture is preferably 22~30 DEG C, more preferably 26
℃.The present invention is not particularly limited the solid-liquid separating method, using manner known in the art, such as filtering or centrifugation
Deng.
It is big in order to precipitate removal that it is 70~80% that the present invention, which adds ethyl alcohol to concentration of alcohol into Cordyceps fermented liquid,
The impurity such as molecular polysaccharide, albumen, and active principle is enriched in supernatant.In the present invention, the revolving speed of the centrifugation is preferred
For 5000~15000rpm, more preferably 10000rpm.In the present invention, the time of the centrifugation is preferably 15~60min, more
Preferably 30min.The present invention is not particularly limited to how to remove ethyl alcohol, is steamed using method commonly used in the art, such as rotation
Hair.The present invention is not particularly limited the mode of the drying, using methods known in the art, such as heated-air drying, subtracts
It press dry dry.
In the present invention, chitosan uses in overlay film liquid as antisepsis antistaling agent.Chitosan is a kind of natural food
Preservative has stronger antibacterial and antioxidation, additionally it is possible to effectively inhibit the respiratory intensity in winter worm summer to increase, delay solvable
Property the nutriments such as solid content and titratable acid be lost, reduce weight-loss ratio, prevent from softening.
In the present invention, chitosan grape saccharide complex plays to form antibacterial, anti-oxidant, inhibition breathing etc. in overlay film liquid
The effect of the protective film of effect.In the present invention, the preparation method of the chitosan grape saccharide complex preferably includes following
Step:
A1, chitosan and glucose are dissolved in glacial acetic acid, are configured to containing 0.5~2% chitosan of volumetric concentration and body
The glacial acetic acid solution of product 0.5~2% glucose of concentration；
A2, the glacial acetic acid solution containing 0.5~2% glucose of 0.5~2% chitosan of volumetric concentration and volumetric concentration is existed
103.4KPa、121.3℃(1.05kg/cm2) under conditions of react 15~20min, it is multiple to obtain chitosan glucose for freeze-drying
Close object.
In the present invention, in the step A1, the glacial acetic acid solution being preferably configured to contains volumetric concentration 1~1.5%
1~1.5% glucose of chitosan and volumetric concentration.In the present invention, the time reacted in the step A2 is preferably 16~
18min。
In the present invention, the time that the cordyceps sinensis of the immersion impregnates in overlay film liquid is preferably 30~200s, more excellent
It is selected as 80~150s.The present invention is not particularly limited the dosage of the overlay film liquid, can submerge cordyceps sinensis.In the present invention
In, the temperature of the drying is preferably 0~4 DEG C, and more preferably 2~3 DEG C.
In the present invention, the solvent of the antistaling agent and filming agent is preferably water.
After obtaining the cordyceps sinensis of overlay film, the cordyceps sinensis of overlay film is packed into packaging bag by the present invention, is filled with by oxygen and two
The mixed gas of carbonoxide composition carries out controlled atmospheric packing, obtains the cordyceps sinensis of controlled atmospheric packing.
In the present invention, the volume ratio of the oxygen in the mixed gas and carbon dioxide is preferably 55~65:35~45,
More preferably 60:40.The method of the invention is carried out using air conditioning packaging equipment.
In the present invention, the material of the packaging bag includes but is not limited to PET/CPE, LDPE or PEIPA/EV OH/PA/
MPE.The realization of controlled atmospheric packing of the present invention uses the device of controlled atmospheric packing, and which is not limited by the present invention.
After obtaining the cordyceps sinensis of controlled atmospheric packing, the present invention carries out ultraviolet irradiation, refrigeration to the cordyceps sinensis of controlled atmospheric packing
?.The present invention provides for this animal of cordyceps sinensis-plant complex it is fresh-keeping required for UV irradiation dose and when
Between, it can satisfy the needs for keeping cordyceps sinensis color, smell and taste for a long time.
In the present invention, the irradiation dose is 2~4KJ/m2, preferably 2.5~3.5KJ/m2；The time of the irradiation
For 10~20min, more preferably 12~18min.UV-C fluorescent tube is used when ultraviolet irradiation of the present invention.
In the present invention, the temperature of the refrigeration is preferably -4~4 DEG C, more preferably 0~3 DEG C.
The method of the fresh-keeping cordyceps sinensis of irradiation method provided by the invention combines composite preservative, covers for cordyceps sinensis surface
The processing of film and controlled atmospheric packing and ultraviolet irradiation can effectively extend the fresh-keeping of cordyceps sinensis, delay cordyceps sinensis fresh goods
Weight-loss ratio growth, the reduction of soluble protein and the growth of polyphenol oxidase activity.
Technical solution provided by the invention is described in detail below with reference to embodiment, but they cannot be understood
For limiting the scope of the present invention.
Embodiment 1
Prepare fresh-keeping liquid: the different Vc of 1mmol/L D-sorbite, 1mmol/L, 6 μm of ol/L phytic acid, L-cysteine concentration are
0.4mg/kg。
The preparation of the ethanol extract of Cordyceps fermented liquid: PDA (Ma Ling is inoculated in after aweto strain is activated
Potato-glucose sugar-agar) in liquid fermentation medium, 7d is cultivated at 26 DEG C, centrifuging and taking supernatant obtains Cordyceps fermented liquid；
The concentration expressed in percentage by volume that ethyl alcohol is added to ethyl alcohol into Cordyceps fermented liquid stops when being 75%, and supernatant is collected by centrifugation, subtracts
Ethyl alcohol in pressure distillation removal supernatant, freeze-drying obtain the ethanol extract of Cordyceps fermented liquid.
The preparation of chitosan grape saccharide complex: 1% chitosan of configuration quality percentage concentration, 1% Portugal of mass percentage concentration
The glacial acetic acid mixed solution of grape sugar, in pressure 103.4kPa (1.05kg/cm2), maintain 15 minutes under the conditions of 121.3 DEG C of temperature,
Reactant freeze-drying, obtains chitosan grape saccharide complex.
Prepare overlay film liquid: 30mg/mL cordyceps mycelia extract, 5mg/mL chitosan oligosaccharide, 5mg/mL chitosan glucose are multiple
Close object.
At 0 DEG C, with head, stroma and the polypide of the linear flowing water cordyceps sinensis with soil collected to the place of production according to
It is secondary to be cleaned；It is ultrasound in 0.1~0.5.% calcium lactate solution that fresh cordyceps sinensis after cleaning, which is placed in mass percentage concentration,
Wave cleans 5 times (250W, frequency 120Hz, 0 DEG C of temperature, time 5min/ times), and 0 DEG C drains surface moisture.
Fresh cordyceps sinensis after ultrasonic cleaning is impregnated into 2min, 0 DEG C of cold wind drying, the winter after being impregnated in fresh-keeping liquid
Worm summer grass.Cordyceps sinensis after immersion is impregnated into 30s in overlay film liquid, 0 DEG C of cold wind drying obtains the cordyceps sinensis of overlay film.
The cordyceps sinensis of overlay film is fitted into packaging bag made of PET/CPE material, with gas-control packing device will by oxygen and
The mixed gas of carbon dioxide volume ratio 55:35 is filled in packaging bag, obtains the cordyceps sinensis of controlled atmospheric packing.With UV-C fluorescent tube,
2KJ/m2Uniform UV irradiates 20min, refrigerates at 3 DEG C.
Embodiment 2
Prepare fresh-keeping liquid: 3mmol/L D-sorbite, 3mmol/LVc, 9 μm of ol/L phytic acid, L-cysteine concentration are
0.8mg/kg。
The preparation of the ethanol extract of Cordyceps fermented liquid: PDA (Ma Ling is inoculated in after aweto strain is activated
Potato-glucose sugar-agar) in liquid fermentation medium, 7d is cultivated at 24 DEG C, centrifuging and taking supernatant obtains Cordyceps fermented liquid；
The concentration expressed in percentage by volume that ethyl alcohol is added to ethyl alcohol into Cordyceps fermented liquid stops when being 70%, and supernatant is collected by centrifugation, subtracts
Ethyl alcohol in pressure distillation removal supernatant, freeze-drying obtain the ethanol extract of Cordyceps fermented liquid.
The preparation of chitosan grape saccharide complex: 1% chitosan of configuration quality percentage concentration, 1% Portugal of mass percentage concentration
The glacial acetic acid mixed solution of grape sugar, in pressure 103.4kPa (1.05kg/cm2), maintain 20 minutes under the conditions of 121.3 DEG C of temperature,
Reactant freeze-drying, obtains chitosan grape saccharide complex.
Prepare overlay film liquid: 100mg/mL cordyceps mycelia extract, 30mg/mL chitosan oligosaccharide, 50mg/mL chitosan glucose
Compound.
At 4 DEG C, with head, stroma and the polypide of the linear flowing water cordyceps sinensis with soil collected to the place of production according to
It is secondary to be cleaned；It is ultrasound in 0.1~0.5% calcium lactate solution that fresh cordyceps sinensis after cleaning, which is placed in mass percentage concentration,
Wave cleans 1 time (power 1200W, frequency 40k HzHz, 0 DEG C of temperature, time 10min/ times), and 4 DEG C drain surface moisture.
Fresh cordyceps sinensis after ultrasonic cleaning is impregnated into 5min, 10 DEG C of cold wind dryings, after being impregnated in fresh-keeping liquid
Cordyceps sinensis.Cordyceps sinensis after immersion is impregnated into 200s in overlay film liquid, 10 DEG C of cold wind dryings obtain the worm summer in winter of overlay film
Grass.
The cordyceps sinensis of overlay film is fitted into packaging bag made of LDPE material, it will be by oxygen and dioxy with gas-control packing device
The mixed gas for changing carbon volume ratio 65:35 is filled in packaging bag, obtains the cordyceps sinensis of controlled atmospheric packing.With UV-C fluorescent tube, 4KJ/
m2Uniform UV irradiates 10min, refrigerates at 4 DEG C.
Embodiment 3
Prepare fresh-keeping liquid: the different Vc of 2mmol/L D-sorbite, 2mmol/L, 7 μm of ol/L phytic acid, L-cysteine concentration are
0.6mg/kg。
The preparation of the ethanol extract of Cordyceps fermented liquid: liquid fermentation training is inoculated in after aweto strain is activated
It supports in base (including potato, soya bean, sucrose, glucose, peptone, yeast extract, mineral salt and vitamin), is cultivated at 27 DEG C
7d, centrifuging and taking supernatant, obtains Cordyceps fermented liquid；The volume basis of ethyl alcohol to ethyl alcohol is added into Cordyceps fermented liquid
Concentration stops when being 80%, and supernatant, the ethyl alcohol in vacuum distillation removal supernatant is collected by centrifugation, and freeze-drying obtains winter worm
The ethanol extract of summer grass fermentation liquid.
The preparation of chitosan grape saccharide complex: 1% chitosan of configuration quality percentage concentration, 1% Portugal of mass percentage concentration
The glacial acetic acid mixed solution of grape sugar, in pressure 103.4kPa (1.05kg/cm2), maintain 16 minutes under the conditions of 121.3 DEG C of temperature,
Reactant freeze-drying, obtains chitosan grape saccharide complex.
Prepare overlay film liquid: 70mg/mL cordyceps mycelia extract, 20mg/mL chitosan oligosaccharide, 30mg/mL chitosan glucose
Compound.
At 5 DEG C, with head, stroma and the polypide of the linear flowing water cordyceps sinensis with soil collected to the place of production according to
It is secondary to be cleaned；It is ultrasonic cleaning in 0.1% calcium lactate solution that fresh cordyceps sinensis after cleaning, which is placed in mass percentage concentration,
3 times (power 700W, frequency 80Hz, 5 DEG C of temperature, time 7min/ times), 2 DEG C drain surface moisture.
Fresh cordyceps sinensis after ultrasonic cleaning is impregnated into 3min, 2 DEG C of cold wind dryings, the winter after being impregnated in fresh-keeping liquid
Worm summer grass.Cordyceps sinensis after immersion is impregnated into 150s in overlay film liquid, 0 DEG C of cold wind drying obtains the cordyceps sinensis of overlay film.
The cordyceps sinensis of overlay film is fitted into packaging bag made of PEIPA/EV OH/PA/MPE material, with gas-control packing device
It will be filled in packaging bag by the mixed gas of oxygen and carbon dioxide volume ratio 60:40, obtain the cordyceps sinensis of controlled atmospheric packing.With
UV-C fluorescent tube, 3KJ/m2Uniform UV irradiates 15min, refrigerates at 2 DEG C.
Embodiment 4
Prepare fresh-keeping liquid: 1mmol/L D-sorbite, 3mmol/LVc, 8 μm of ol/L phytic acid, L-cysteine concentration are
0.7mg/kg。
The preparation of the ethanol extract of Cordyceps fermented liquid: liquid fermentation training is inoculated in after aweto strain is activated
It supports in base (including in potato, soya bean, sucrose, glucose, peptone, yeast extract, mineral salt and vitamin), is cultivated at 25 DEG C
7d, centrifuging and taking supernatant, obtains Cordyceps fermented liquid；The volume basis of ethyl alcohol to ethyl alcohol is added into Cordyceps fermented liquid
Concentration stops when being 80%, and supernatant, the ethyl alcohol in vacuum distillation removal supernatant is collected by centrifugation, and freeze-drying obtains winter worm
The ethanol extract of summer grass fermentation liquid.
The preparation of chitosan grape saccharide complex: 1% chitosan of configuration quality percentage concentration, 1% Portugal of mass percentage concentration
The glacial acetic acid mixed solution of grape sugar, in pressure 103.4kPa (1.05kg/cm2), maintain 16 minutes under the conditions of 121.3 DEG C of temperature,
Reactant freeze-drying, obtains chitosan grape saccharide complex.
Prepare overlay film liquid: 70mg/mL cordyceps mycelia extract, 20mg/mL chitosan oligosaccharide, 30mg/mL chitosan glucose
Compound.
At 4 DEG C, with head, stroma and the polypide of the linear flowing water cordyceps sinensis with soil collected to the place of production according to
It is secondary to be cleaned；It is ultrasonic cleaning in 0.1% calcium lactate solution that fresh cordyceps sinensis after cleaning, which is placed in mass percentage concentration,
4 times (power 400W, frequency zones 100Hz, 4 DEG C of temperature, time 6min/ times), 2 DEG C drain surface moisture.
Fresh cordyceps sinensis after ultrasonic cleaning is impregnated into 4min, 2 DEG C of cold wind dryings, the winter after being impregnated in fresh-keeping liquid
Worm summer grass.Cordyceps sinensis after immersion is impregnated into 180s in overlay film liquid, 3 DEG C of cold wind dryings obtain the cordyceps sinensis of overlay film.
The cordyceps sinensis of overlay film is fitted into packaging bag made of PET/CPE material, with gas-control packing device will by oxygen and
The mixed gas of carbon dioxide volume ratio 58:42 is filled in packaging bag, obtains the cordyceps sinensis of controlled atmospheric packing.With UV-C fluorescent tube,
4KJ/m2Uniform UV irradiates 12min, refrigerates at 2 DEG C.
Embodiment 5
Prepare fresh-keeping liquid: 1.5mmol/L D-sorbite, 2.5mmol/LVc, 7.5 μm of ol/L phytic acid, L-cysteine concentration
For 0.65mg/kg.
The preparation of the ethanol extract of Cordyceps fermented liquid: liquid fermentation training is inoculated in after aweto strain is activated
It supports in base (including in potato, soya bean, sucrose, glucose, peptone, yeast extract, mineral salt and vitamin), is cultivated at 28 DEG C
7d, centrifuging and taking supernatant, obtains Cordyceps fermented liquid；The volume basis of ethyl alcohol to ethyl alcohol is added into Cordyceps fermented liquid
Concentration stops when being 77%, and supernatant, the ethyl alcohol in vacuum distillation removal supernatant is collected by centrifugation, and freeze-drying obtains winter worm
The ethanol extract of summer grass fermentation liquid.
The preparation of chitosan grape saccharide complex: 1% chitosan of configuration quality percentage concentration, 1% Portugal of mass percentage concentration
The glacial acetic acid mixed solution of grape sugar, in pressure 103.4kPa (1.05kg/cm2), maintain 18 minutes under the conditions of 121.3 DEG C of temperature,
Reactant freeze-drying, obtains chitosan grape saccharide complex.
Prepare overlay film liquid: 85mg/mL cordyceps mycelia extract, 17mg/mL chitosan oligosaccharide, 22mg/mL chitosan glucose
Compound.
At 9 DEG C, with head, stroma and the polypide of the linear flowing water cordyceps sinensis with soil collected to the place of production according to
It is secondary to be cleaned；It is ultrasonic cleaning in 0.1% calcium lactate solution that fresh cordyceps sinensis after cleaning, which is placed in mass percentage concentration,
2 times (power 750W, frequency zones 80Hz, 6 DEG C of temperature, time 7min/ times), 1 DEG C drains surface moisture.
Fresh cordyceps sinensis after ultrasonic cleaning is impregnated into 5min, 1 DEG C of cold wind drying, the winter after being impregnated in fresh-keeping liquid
Worm summer grass.Cordyceps sinensis after immersion is impregnated into 70s in overlay film liquid, 1 DEG C of cold wind drying obtains the cordyceps sinensis of overlay film.
The cordyceps sinensis of overlay film is fitted into packaging bag made of PEIPA/EV OH/PA/MPE material, with gas-control packing device
It will be filled in packaging bag by the mixed gas of oxygen and carbon dioxide volume ratio 61:39, obtain the cordyceps sinensis of controlled atmospheric packing.With
UV-C fluorescent tube, 2KJ/m2Uniform UV irradiates 18min, refrigerates at 1 DEG C.
Comparative example 1
" storing and fresh-keeping method of fresh cordyceps sinensis " recorded according to Chinese patent 201611222582.3 is to the fresh winter worm summer
Grass carries out fresh-keeping:
The fresh cordyceps sinensis just excavated is taken, is cleaned under 5 DEG C of environment temperatures with 5 DEG C of water respectively, then in 8 DEG C of environment
Under be less than 0.5g, 0.5~1.0g by weight, fall into three classes greater than 1.0g.
Cordyceps sinensis by weight less than 0.5g saves under -5 DEG C, 75% damp condition；
The cordyceps sinensis of 0.5~1.0g of weight is saved under -7 DEG C, 70% damp condition；
Cordyceps sinensis by weight greater than 1.0g saves under -10 DEG C, 60% damp condition.
Embodiment 6
1, process is tested
Take fresh cordyceps sinensis 180 of 0.5~1.0g specification respectively, be randomly divided into 6 groups, respectively according to Examples 1 to 5 and
The method of comparative example 1 is fresh-keeping to the progress of each group fresh cordyceps sinensis, and measurement each group cordyceps sinensis is relative to Preservation Treatment after saving 75d
Preceding weight-loss ratio, soluble protein content rate of descent and polyphenol oxidase activity growth rate.Testing result is as shown in table 1.
Sample weight loss rate is according to " Wang Jingbo waits influence [J] of .3 kind plants essential oil suffocating treatment to nectarine fresh-keeping effect
Nuclear agricultural science report, 2018,32 (5): the method measurement in 933-940. "；Soluble protein content rate of descent is according to " Deng Lili waits
Bright trip's method measurement condition optimizing [J] the Food Science of apple tissue trace soluble egg from content of coomassie, 2012, (24):
185-189. " method measurement；Polyphenol oxidase activity growth rate is according to " Jiankang waits postharvest fruit and vegetable bio-chemical characteristics to instruct
Beijing [M]: China Light Industry Press, the measurement of 2007:59-154. " method.
The results are shown in Table 1, when saving the 75th day, the fresh-keeping cordyceps sinensis of the method for the present invention, in weight-loss ratio, solubility
In terms of protein content rate of descent and polyphenol oxidase activity growth rate, it is fresh-keeping to be superior to 201611222582.3 method of Chinese patent
Cordyceps sinensis.Meanwhile the fresh-keeping effect of the method for the invention is more obvious with the extension advantage of fresh keeping time.
Table 1 saves the 75th day, weight-loss ratio, soluble protein content rate of descent and the polyphenol of each processing group cordyceps sinensis sample
The measurement result of oxidase active growth rate
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications are also answered
It is considered as protection scope of the present invention.
Claims (10)
1. a kind of method of controlled atmospheric packing and ultraviolet irradiation composite fresh-keeping cordyceps sinensis, comprising the following steps:
(1) fresh cordyceps sinensis is soaked in fresh-keeping liquid, takes out drying, the cordyceps sinensis after being impregnated；
The fresh-keeping liquid includes 1~3mmol/L D-sorbite, 1~3mmol/L vitamin C or ISOASCORBIC ACID, 6~9 μm of ol/L
The L-cysteine concentration of phytic acid, 0.4~0.8mg/kg；
(2) cordyceps sinensis after immersion is soaked in overlay film liquid, takes out drying, obtains the cordyceps sinensis of overlay film；
The overlay film liquid include the ethanol extract of 30~100mg/ml Cordyceps fermented liquid, 5~30mg/L chitosan oligosaccharide and 5~
50mg/L chitosan grape saccharide complex；
(3) cordyceps sinensis of overlay film is packed into packaging bag, is filled with and controlled atmosphere is carried out by the mixed gas that oxygen and carbon dioxide form
Packaging, obtains the cordyceps sinensis of controlled atmospheric packing；
(4) ultraviolet irradiation, refrigeration are carried out to the cordyceps sinensis of controlled atmospheric packing；
The irradiation dose is 2~4KJ/m2, irradiation time is 10~20min.
2. the method according to claim 1, wherein the fresh cordyceps sinensis is by ultrasonic cleaning in step (1)
It is soaked in fresh-keeping liquid again.
3. according to the method described in claim 2, ultrasound is clear it is characterized in that, the clear power of the ultrasound is 250~1200W
The frequency washed is 40kHz~120kHz, and the number of ultrasonic cleaning is 2~5 times, the time being cleaned by ultrasonic every time independently is 5~
10min。
4. the method according to claim 1, wherein the ethyl alcohol of the Cordyceps fermented liquid mentions in step (1)
Take the preparation method of object the following steps are included:
S1, cordyceps sinensis is inoculated in liquid fermentation medium, cultivates 6~8d, be separated by solid-liquid separation, obtain cordyceps sinensis fermentation
Liquid；
S2, the concentration expressed in percentage by volume that ethyl alcohol to ethyl alcohol are added into Cordyceps fermented liquid reach 70~80%, are centrifuged to obtain supernatant
Liquid removes drying after ethyl alcohol, obtains the ethanol extract of Cordyceps fermented liquid.
5. according to the method described in claim 4, it is characterized in that, the liquid fermentation liquid in the step S1 include potato,
One of soya bean, sucrose, glucose, peptone, yeast extract, mineral salt and vitamin are a variety of.
6. the method according to claim 1, wherein in step (1), the system of the chitosan grape saccharide complex
Preparation Method the following steps are included:
A1, chitosan and glucose are dissolved in glacial acetic acid, are configured to dense containing 0.5~2% chitosan of volumetric concentration and volume
Spend the glacial acetic acid solution of 0.5~2% glucose；
A2, the glacial acetic acid solution containing 0.5~2% glucose of 0.5~2% chitosan of volumetric concentration and volumetric concentration is existed
103.4KPa, 15~20min is reacted under conditions of 121.3 DEG C, freeze-drying obtains chitosan grape saccharide complex.
7. the method according to claim 1, wherein the soaking time in the step (1) is 2~5min.
8. method according to claim 1 or claim 7, which is characterized in that soaking time in the step (2) is 30~
200s。
9. the method according to claim 1, wherein in the step (3), oxygen and titanium dioxide in mixed gas
The volume ratio of carbon is 55~65:35-45.
10. the method according to claim 1, wherein the refrigerated storage temperature in the step (4) is -4~4 DEG C.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910175607.6A CN109717347A (en) | 2019-03-08 | 2019-03-08 | A kind of method of controlled atmospheric packing and ultraviolet irradiation composite fresh-keeping cordyceps sinensis |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910175607.6A CN109717347A (en) | 2019-03-08 | 2019-03-08 | A kind of method of controlled atmospheric packing and ultraviolet irradiation composite fresh-keeping cordyceps sinensis |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109717347A true CN109717347A (en) | 2019-05-07 |
Family
ID=66302115
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910175607.6A Withdrawn CN109717347A (en) | 2019-03-08 | 2019-03-08 | A kind of method of controlled atmospheric packing and ultraviolet irradiation composite fresh-keeping cordyceps sinensis |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109717347A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110447805A (en) * | 2019-09-04 | 2019-11-15 | 贵州省普安县普白珍稀资源开发有限公司 | A kind of Rhizoma Gastrodiae preservation method |
| CN110628633A (en) * | 2019-10-31 | 2019-12-31 | 东莞市东阳光冬虫夏草研发有限公司 | Fresh-keeping method of fresh cordyceps sinensis and fresh cordyceps sinensis product |
| CN111990578A (en) * | 2020-08-26 | 2020-11-27 | 尹浩 | Fresh freezing storage method of natural cordyceps sinensis |
| CN112753756A (en) * | 2020-12-29 | 2021-05-07 | 裘名田 | Preservation method of cordyceps sinensis |
| CN112931747A (en) * | 2021-02-05 | 2021-06-11 | 西南科技大学 | High-energy electron beam irradiation storage process for radix angelicae |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20060159809A1 (en) * | 2005-01-19 | 2006-07-20 | Myers Bigel Sibley & Sajovec, P.A. | Packaged products of mushrooms contained in a modified gas atmosphere and methods and apparatus for making the same |
| CN102406164A (en) * | 2011-09-29 | 2012-04-11 | 青海仁济冬虫夏草有限公司 | Method for processing living plantworms and preparing plantworms can |
| CN104890931A (en) * | 2014-03-04 | 2015-09-09 | 邓彬 | Controlled atmosphere storage process of such fresh Chinese herbal medicines as fresh cordyceps, fresh ginseng and fresh gastrodia elata |
| CN106727734A (en) * | 2017-01-05 | 2017-05-31 | 广东东阳光药业有限公司 | Fresh cordyceps sinensis product and preparation method thereof |
-
2019
- 2019-03-08 CN CN201910175607.6A patent/CN109717347A/en not_active Withdrawn
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20060159809A1 (en) * | 2005-01-19 | 2006-07-20 | Myers Bigel Sibley & Sajovec, P.A. | Packaged products of mushrooms contained in a modified gas atmosphere and methods and apparatus for making the same |
| CN102406164A (en) * | 2011-09-29 | 2012-04-11 | 青海仁济冬虫夏草有限公司 | Method for processing living plantworms and preparing plantworms can |
| CN104890931A (en) * | 2014-03-04 | 2015-09-09 | 邓彬 | Controlled atmosphere storage process of such fresh Chinese herbal medicines as fresh cordyceps, fresh ginseng and fresh gastrodia elata |
| CN106727734A (en) * | 2017-01-05 | 2017-05-31 | 广东东阳光药业有限公司 | Fresh cordyceps sinensis product and preparation method thereof |
Non-Patent Citations (5)
| Title |
|---|
| 中国食品工业协会食品安全师培训管理办公室组织编写: "《食品安全基础知识》", 31 May 2008, 中国商业出版社 * |
| 张文华等: "《生物化学与微生物学》", 30 June 2012, 海洋出版社 * |
| 梅全喜主编: "《现代中药药理与临床应用手册》", 31 October 2016, 中国中医药出版社 * |
| 汪多仁编著: "《有机食品营养强化剂》", 31 August 2008, 科学技术文献出版社 * |
| 马佩选等: "《葡萄酒分析与检验》", 28 February 2017, 中国轻工业出版社 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110447805A (en) * | 2019-09-04 | 2019-11-15 | 贵州省普安县普白珍稀资源开发有限公司 | A kind of Rhizoma Gastrodiae preservation method |
| CN110628633A (en) * | 2019-10-31 | 2019-12-31 | 东莞市东阳光冬虫夏草研发有限公司 | Fresh-keeping method of fresh cordyceps sinensis and fresh cordyceps sinensis product |
| CN111990578A (en) * | 2020-08-26 | 2020-11-27 | 尹浩 | Fresh freezing storage method of natural cordyceps sinensis |
| CN112753756A (en) * | 2020-12-29 | 2021-05-07 | 裘名田 | Preservation method of cordyceps sinensis |
| CN112931747A (en) * | 2021-02-05 | 2021-06-11 | 西南科技大学 | High-energy electron beam irradiation storage process for radix angelicae |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN109717347A (en) | A kind of method of controlled atmospheric packing and ultraviolet irradiation composite fresh-keeping cordyceps sinensis | |
| CN109717346A (en) | A kind of method of the fresh-keeping cordyceps sinensis of irradiation method | |
| CN103932355B (en) | Dendrobium candidum preservation method | |
| CN110074178B (en) | Modified atmosphere preservation method of instant pomegranate seeds | |
| CN108094519B (en) | Grape antagonistic yeast composite biological preservative and preparation method and application thereof | |
| KR101559543B1 (en) | Manufacturing method of salted fish using Dendropanax morbifera | |
| CN110326664A (en) | A kind of rice with remained germ and the method for extending its shelf-life | |
| CN103300141A (en) | Peach low-temperature storage and fresh keeping method and application of gamma-aminobutyric acid to fresh keeping of peaches | |
| CN109717348A (en) | A method of the fresh-keeping cordyceps sinensis of controlled atmospheric packing containing argon gas | |
| CN107484816A (en) | A kind of Chinese toon storage fresh-keeping and processing method | |
| CN113016986B (en) | Method for improving flavor substances of day lily | |
| MEDANY | Cultivation possibility of golden oyster mushroom (Pleurotus citrinopileatus) under the Egyptian conditions | |
| CN109717356B (en) | Fresh cordyceps sinensis quick-freezing preservation method combining liquid nitrogen with liquid carbon dioxide | |
| CN106578029A (en) | Method for coating preservation of Litchi chinensis bacterium by phellinus igniarius polysaccharide | |
| US11259552B2 (en) | Chips formed by slicing with apple cell clean water jet scalpel and differential temperature combined with differential pressure puffing and processing technology | |
| CN109744457B (en) | Liquid nitrogen spraying type quick-freezing method for fresh cordyceps sinensis | |
| CN111449130B (en) | Green preservative for fresh-cut Nanguo pear | |
| CN110432457B (en) | Dried and crisp black persimmon slices and processing technology | |
| CN113180213B (en) | Fresh-keeping method for prolonging shelf life of fresh-cut asparagus | |
| CN110226622A (en) | Lichee fresh-keeping storage practice based on space electric field | |
| CN107771929A (en) | A kind of spraying confrontation storage method that can extend the preserving rice phase | |
| CN114365762A (en) | Spinach preservation and preservation method and preparation method thereof | |
| CN101044871B (en) | Method for canning and preserving blechnoid | |
| CN112167317A (en) | Aquatic product water-retaining preservative and freezing preservation method | |
| CN109662137A (en) | A kind of electron beam irradiation green asparagus preservation method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WW01 | Invention patent application withdrawn after publication | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20190507 |